WO2009035062A1 - 試料充填装置 - Google Patents
試料充填装置 Download PDFInfo
- Publication number
- WO2009035062A1 WO2009035062A1 PCT/JP2008/066478 JP2008066478W WO2009035062A1 WO 2009035062 A1 WO2009035062 A1 WO 2009035062A1 JP 2008066478 W JP2008066478 W JP 2008066478W WO 2009035062 A1 WO2009035062 A1 WO 2009035062A1
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- WIPO (PCT)
- Prior art keywords
- sample
- microchip
- chamber
- package
- sample chamber
- Prior art date
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
- B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00089—Magazines
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
- G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow
Definitions
- the present invention has a plurality of reaction tanks and sample tanks used for gene analysis and the like, and further, samples are collected in a microchip in which the reaction tanks and the sample tanks are connected by a fine flow path.
- the present invention relates to a sample filling device for injecting into a mouthpiece tip.
- Background Art Book Recently, “Biochemical Micro Chemical Analysis System” Shoichi Shoji, Waseda University (Non-Patent Literature)
- Patent Document 2 a microchip in which a filling container and a fine channel are provided on a single chip, Various liquid feeding mechanisms and methods for gene analysis are being researched on chips, microreactors, fluid device chips, and chips called chemical reaction cartridges that control and feed samples and liquid samples. .
- Non-Patent Document 1 “a conceptual diagram of a system integrated on a single substrate is a sample introduction mechanism, carrier solution, pump / reactor mixing / reactor with sample flow control, components A microchip has been proposed as a “hybrid system where each element is micronized, a flow path is formed inside, and the elements are arranged on a substrate and connected via an O-ring”. Yes. Furthermore, the introduction of samples required for analysis on the microchip is performed from the outside via a micropump.
- Patent Document 2 “a sample container that receives a sample from the outside in a chemical reaction cartridge that performs a chemical reaction by transferring or sealing the contents by deformation when an external force is applied”. A chemical reaction cartridge has been proposed. Further, Patent Document 2 describes that “the sample is injected using a syringe needle” in the process of injecting the sample at the initial stage. Disclosure of the invention:
- Non-Patent Document 1 has means for injecting a sample from the outside of the microchip into a sample tank in the microchip using a micropump or the like.
- liquid feeding means controlled with high precision is required outside the microchip.
- the analysis process changed, it was necessary to clean and replace the entire liquid delivery means.
- the analysis of minute sump nare requires a highly accurate micro-volume delivery device, which is expensive and requires a large installation space.
- the chemical reaction cartridge shown in Patent Document 2 uses a means such as an injection needle as a means for first injecting a sample or a sample.
- a means such as an injection needle as a means for first injecting a sample or a sample.
- sample selection mistakes, sample injection mistakes, reagent amount mistakes, air contamination, etc. may occur due to artificial mistakes, and the reagent may drip and cause cross-contamination called contamination. was there.
- sample bottles that require refrigerated storage must be removed from the storage box each time, and the required amount must be collected and returned to the storage box, which may adversely affect the sample as the temperature changes. there were.
- An object of the present invention is to provide a sample filling device that is a simple mechanism that can be taken out and injected from a storage environment in an amount, and that is inexpensive and downsized.
- the present invention has at least a sample receiving tank and a reaction tank and a flow path connected between the sample receiving tank and the reaction tank, and a fine component contained in the sample.
- a sample filling device that fills a microchip for performing a reaction
- the sample receiving chamber is filled with the sample in the sample chamber by mounting the package having the sample chamber filled with the sample in advance on the microchip.
- FIG. 1 is a cross-sectional perspective view showing a configuration of a microchip sample transfer mechanism in an embodiment of the present invention.
- FIG. 2 is a perspective view showing a mechanism configuration of the microchip and the sample package in the embodiment of the present invention.
- FIG. 3A is a cross-sectional view showing a mechanism configuration of the sample package in the embodiment of the present invention.
- FIG. 3B is a plan view showing the mechanism configuration of the sample package in the embodiment of the present invention.
- FIG. 4A is a cross-sectional view showing the operation of the sample package in the embodiment of the present invention.
- FIG. 4B is a cross-sectional view showing the operation of the sample package in the embodiment of the present invention.
- FIG. 4C is a cross-sectional view showing the operation of the sample package in the embodiment of the present invention.
- FIG. 5 is a sectional view showing another embodiment of the sample package according to the present invention.
- FIG. 6 is a cross-sectional view showing still another embodiment of the sample package according to the present invention. Best Mode for Carrying Out the Invention:
- FIG. 1 is a perspective view showing the configuration of an apparatus for introducing a sample into a microchip and reacting the sample using the sample filling apparatus (reagent introduction mechanism) according to the present invention.
- the pneumatic circuit is indicated by J I S logic symbols.
- the machine frame 1 is provided with a table 3 via a support column 2, and the table 3 is provided with a disposal hole 5 whose periphery is sealed with an O-ring 6.
- the disposal hole 5 is connected to a disposal tank 8 provided on the machine frame 1 through a disposal solenoid valve 7 and a tube 7a.
- Te On the upper surface of the table 3, pins 10a and 10b are provided in a convex shape so as to be aligned with pin holes 55a and 55b provided in the microchip 50 and to guide them to predetermined positions.
- table 3 is connected with hinge screw 9 via fastening screw 25, pressurizing holes 22a, 22b, 22c, 22d, 22e sealed around by O-ring 26 and surroundings by O-ring 27.
- the pressure holes 22a, 22b, 22c, 22d, 22e provided through the cover 20 are respectively connected to the tubes 17a, 17b, 17c, 17d, 17e.
- the pressure solenoid valve 16a, 16b, 16c, 16d, 16e is connected to the secondary side.
- shatter pressure holes 23a, 23b, 23c, 23d, 23e, and 23f are connected to the shatter solenoid valve 1 8 by tubes 1 9a, 19b, 19c, 19d, 19e, and 19f, respectively.
- the secondary side of a, 18 b, 18 c, 18 d, 18 e, 18 f and the air supply hole 24 are connected to the secondary side of the air supply solenoid valve 28 by a tube 29.
- Pressurized solenoid valve 16 a, 16 b, 16 c, 16 d, 16 e and shatter solenoid valve 18 a, 18 b, 18 c, 18 d, 18 e, 18 f and primary side of air supply solenoid valve 28 Is connected to the accumulator 1 1.
- the accumulator 11 is connected to a pump 12 driven by a motor 13 and a pressure sensor 14 for detecting internal pressure.
- the table 3 is provided with a temperature adjusting unit 30 that controls a predetermined part of the microchip 50 from the lower surface to a predetermined temperature.
- the controller 15 that executes a preset program includes pressurizing solenoid valves 16 a, 16 b, 16 c, 16 d, 16 e and a waste solenoid valve 7, shatter solenoid valves 18 a, 1 8 b, 18 c, 18 d, 18 e, 18 f and air supply solenoid valve 28 are connected so as to be able to control the operation.
- the controller 15 has a pump 12 so that the pressure in the pressure accumulator 11 can be controlled to a predetermined pressure.
- a pressure sensor 14 is connected to detect the pressure in the motor 13 and the pressure accumulator 11 and perform feedback. With the above configuration, the pressure in the pressure accumulator 11 is always kept at a predetermined pressure by a command from the controller 15.
- the temperature adjustment unit 30 is controlled. It is connected to the roller 15 to perform pre-programmed temperature control.
- the sample package 100 is inserted into the sample receiving tanks 52a, 52b and 52c on the convex sample chambers 101a, 101b and 101c force S microchip 50. Further, the microchip 50 is configured such that pin holes 55 a and 55 b are guided by the pins 10 a and 10 b, mounted on the table 3, and clamped by the cover 20 by the fastening screws 25.
- FIG. 2 is a perspective view showing details of the sample package 100 and the microchip 50 showing the embodiment of the present invention.
- the microchip 50 has a multilayer structure in which a main plate 51a, a second plate 51b, a third plate 51c, and a fourth plate 51d each made of a stretchable resin are bonded together.
- a concave shape is formed through the main plate 51a and the second plate 51b, sample receiving tanks 52a, 52b, 52c and an air supply port 54 are provided.
- a reaction tank 52 d, a concave tank 52 e, an extraction tank 52 e, and an amplification tank 58 &, 58 b, 58 c are provided.
- a shirt taro 53a, 53b, 53c, 53d, 53e, 53 which has a concave shape penetrating the main plate 51a, the second plate 51b, and the third plate 51c. f is provided.
- the disposal hole 56 is provided so as to penetrate the second plate 51 b, the third plate 51 c, and the fourth plate 51 d downward.
- Sample receiving tanks 52 a, 52 b, 52 c are pressurized holes 2 2 a, 22 b, 22 c
- Reaction tank 52 d is pressurized holes 22 d
- Extraction tank 52 e is pressurized holes 22 e
- Shirt Taro 53 a, 53 b, 53 c, 53 d, 53 e, 53 f are mounted at positions that match the shutter pressure holes 23 a, 23 b, 23 c, 23 d, 23 e, 23 f. Yes.
- the sample receiving tank 52 a, 52 b, 53 c, the reaction tank 52 d, the extraction tank 52 e, the PCR widening tank 58 a, 58 b, 58 c, and the air supply port 54 are connected to the main plate 51.
- the flow path 61 a, 61 b, 61 c, 61 d, 61 e, 61 f, 61 g, 61 h, 61 i connected between the a and the second plate 51 b Yes.
- the shutters 53 a, 53 b, 53 c, 53 d, 53 e, 53 f are the shatter channels 62 a, 62 b, 62 c formed between the second plate 51 b and the third plate 52 c. 62 d, 62 e, 62 f and the tip of the channel 61 a, 61 b, 61 c, 61 d, 61 e, 61 f, 61 g, 61 h, 6 1 i and 3rd It is provided so as to cross the plate 51c.
- the flow paths 61a, 61b, 61c, 61d, 61f, 61f, 61g, 61h, 61i are constituted by the second plate 51b and the third plate 51.
- adhering c it is configured in a state where it can be peeled off without adhering the portion to be the flow path.
- shatter flow paths 62 a, 62 b, 62 c, 62 d, 62 e, 62 f should become flow paths when the third plate 51 c and the fourth plate 51 d to be configured are bonded. It is configured in a state where the parts can be peeled off without bonding.
- the second plate 5 1 b and the third plate 51 c inside the concave vessel of the reaction tank 52 d and the extraction tank 52 e are not bonded in the same manner, and the flow paths 61 a, 61 b, 61 c , 61d, 61e, 61f, 61g, 61h, 61i.
- an adsorbing member 60 for extracting a desired fine component is solid-phased in the non-adhesive portion formed between the second plate 51 b and the third plate 51 c inside the reaction tank 52d. Yes.
- the controller 15 sequentially pressurizes the compressed air from the accumulator 1 1 according to a preset program, and pressurizes solenoid valves 1 6 a, 16 b, 16 c, 16 d, 16 e, and waste solenoid valve 7 SHOTTA solenoid valve 1 8 a, 1 8 b, 18 c, 1 8 d, 18 e, 18 f and air supply solenoid valve 28 and pressurization hole 22 a, 22 b ⁇ 22 c and Supply to shutter pressure hole 23a, 23b, 23c, 23d, 23e, 23f, and sample receiving tank 52a, 52b, 53c, reaction tank 52d, extraction tank 52e of microchip 50 PCR amplification tanks 58 a, 58 b, 58 c and air supply port 54.
- the compressed air flows into the sample chambers 101 a, 101 b, 10 of the sample package 100 inserted into the sample receiving tanks 52 a, 52 b, 52 c of the microchip 50. It is supplied to the upper part of 1c and has a mechanism for transferring the internal sample to the channels 61a, 61d, 61c of the microchip 50.
- the detailed control operation is not directly related to the part of the present invention, and the description thereof is omitted.
- FIG. 3A shows a cross-sectional view of the sample package 100
- FIG. 3B shows an arrow view from the Z direction in FIG. 3A.
- the sample package 100 is provided with convex sample chambers 101 a, 101 b, 101 c on the main body plate 104, filled with samples 102 a, 102 b, 102 c, and sealed with a coating 103 made of an elastic material. Has been. At the bottom of each of the sample chambers 10 1 a, 101 b, and 1101 c, a smashed portion 106 and a protruding portion 105 that are partially thin are provided. In the periphery of the sample chambers 101 a, 101 b, 101 c of the main body plate 104, an advantageous blade-like seal portion 107 is provided.
- FIG. 3B shows a part of the sample package 100.
- the seal portion 107 provided on the main body plate 104 of the sample package 100 is provided so as to circulate around the sample chambers 101 a, 101 b, and 101 c. Further, the portion to be destroyed 106 is near the protrusion 105. It is provided in a U shape around. In addition, when physical force is applied to the protruding portion 105 from the outside, the smashed portion 106 is broken so that it is pushed into the sample chambers 101 a, 101 b, and 101 c, and the sample chamber 101 a shown in FIG. The sample 102a, 102b, 102c force S filled inside the 10 lb, 101c is opened to the outside.
- FIGS. 4A to 4C the movement at the time of sample introduction will be described with reference to FIGS. 4A to 4C.
- the microchip 50 is mounted on the table 3, and the sample chambers 101a, 101b, 101c of the sample package 100 are inserted into the sample receiving tanks 52a, 52b, 52c on the microchip 50. Indicates the state that has been performed. At that time, the seal portion 107 and the protrusion portion 105 of the sample package 100 are in contact with one end of the microchip 50.
- FIG. 4B the operation during sample introduction will be described with reference to FIG. 4B.
- the sample package 100 is pressed in the C direction by the O-ring 26 provided on the cover 20. Also, sample package The projecting portion 105 of the di 100 and the sample receiving tank 5 2 a, 5 2 b, 5 2 c of the microchip 50 are also pressed in the C direction. As a result, the sample chambers 1 0 1 a, 1 0 1 b, 1 0 lc are further inserted into the sample receiving tanks 5 2 a, 5 2 b, 5 2 c, and the projections 1 0 5 are not broken.
- Destroy part 1 0 6 As a result, the samples 1 0 2 a, 10 2, and 10 2 c are opened to the sample receiving tanks 5 2 a, 5 2 b, and 5 2 c through the portion to be destroyed 10 6. Similarly, the seal portion 10 07 is pressed against the main plate 51a made of an elastic member constituting the microchip 50, and the blade-like seal portion 107 is bitten into the main plate 51a, A minute gap 1 08 composed of the receiving tanks 52 a, 52 b, 52 c and the sample chambers 10 1 a, 1 0 1 b, 1 0 1 c is sealed from the outside.
- FIG. 4C from the state of FIG. 4B, compressed air is applied in the direction D from the pressure holes 2 2 a, 22 b, and 22 c provided in the cover 20 by the apparatus and control means described in FIG. As a result, the coating 10 0 3 made of an elastic material is bent, and the samples 1 0 2 a, 1 0 2 b, 1 0 2 c are passed through the smashed part 1 0 6 to the sample chamber 1 0 1 a, 1 0 Push it out of 1 b and 1 0 1 c.
- the flow paths 6 la, 6 1 d, and 6 1 e which are partly non-adhered between the second plate 5 1 b and the third plate 5 1 c constituting the microchip 50, are shown in the figure. Opened by the device and control means described in 1. Furthermore, the fine gap 1 08 composed of the sample receiving tank 5 2 a, 5 2 b, 5 2 c and the sample chamber 1 0 1 a, 1 0 1 b, 1 0 1 c is the upper seal part 1 0 Sealed by 7. In other words, samples 1 0 2 a, 1 02 b, 1 0 2 c are mixed with a spatial medium typified by air through the only open channels 6 l a , 6 1 d, 6 1 e. Without injection in the E direction of the microchip 50. Next, another embodiment is shown in FIG.
- FIG. 5 is provided with a biston-like movable body 109 in which the inner walls of the sample chambers 101a, 101b, and 101c are sealed in place of the coating 103 shown in FIG. 3A.
- the movable body 109 pushes the samples 102a, 102b, and 102c through the smashed portion 106, and the same as described above.
- the type of means for extruding from the reagent chambers 101a, 101b, and 101c is not limited.
- FIG. 6 shows an example in which a convex protrusion 110 is provided on the contact surface of the microchip 50 in place of the protrusion 105 shown in FIG. 3A.
- the installation place of a convex-shaped projection part is not limited.
- the gap portion 108 has been described as an insertion gap shape, but the same effect can be obtained with a container shape having a volume. That is, in the present invention, the shape of the gap portion 108 is not limited.
- the same effect can be obtained with other pressurized media (for example, liquid, gel, powder) or the like.
- the type of the compression medium is not limited.
- the destruction target portion 106 is described as a U shape around the vicinity of the projection portion 105.
- the same effect can be obtained in other shapes such as a U shape and a circular shape.
- the shape of the fractured portion 106 is not limited.
- the sharp blade-shaped seal portion 107 in FIGS. 4A to 4C is provided in the sample package 100, but the same effect can be obtained by providing it on the contact surface of the microchip 50. It is done.
- the installation location of the seal portion 107 is not limited.
- the closing operation of the cover 20 is used as the smashing means of the smashed portion 106, but the same effect can be obtained even if it is inserted by manual operation or other driving means.
- the method of the destruction means of the smashed portion 106 is not limited.
- a predetermined amount of sample to be injected is filled.
- Packaged containers can be easily installed in a predetermined storage position in the microchip, preventing operator errors, allowing efficient use of samples, and storing samples other than necessary The state is maintained.
- the upper surface of the package is sealed with a film made of an elastic member, and a pressure medium is applied after mounting the device to improve the accuracy of the injection amount.
- a movable part (pressing member) is provided inside the package, and the pressure medium is pressurized and applied after the device is mounted to move the movable part and improve the accuracy of the injection amount of the internal sample. To do.
- a fractured part made of a material or a mechanically weak part is provided at the bottom of the package, and the bottom of the container forming the package or the receiving part of the package of the microchip is projected to promote the destruction of the non-destructive part.
- the part to be destroyed is destroyed with a small force and the sample is released by concentrating and receiving the physical force when it is mounted on the microchip.
- a sealing part (packing part) is provided between the package and the microchip, and when the pressure medium is pressurized and applied to the knocker, the sealing part prevents leakage, thereby improving the injection accuracy into the microchip.
- the gap when the package is mounted on the microchip has a capacity so that a medium typified by the air present at the time of mounting can be accumulated, so that the flow path of the microchip
- the structure is designed to prevent the contamination and improve the injection accuracy.
- a pressure medium is applied to the top of the package and the coating made of an elastic material is bent, so that a constant amount of sample can be transferred at all times, preventing mistakes and variations in the amount of sample injected by the operator. Analyzing accuracy can be improved.
- the movement of the fixing means performed when the package is mounted on the microchip can be transmitted to the package, and the part to be destroyed can be destroyed and the filled sample can be released automatically or semi-automatically into the microchip. This makes it possible to improve work efficiency and prevent cross-contamination.
- the air remaining when the package is mounted on the microchip is pushed up along the outer wall of the package by capillary action when the ruptured part is destroyed and the filled sample is released for analysis. It is possible to avoid mixing the microchip into the flow path that hinders the analysis, thereby improving the analysis accuracy. Furthermore, by providing a seal between the package and the microchip, the pressure medium can be prevented from leaking when the pressure medium is applied to the top of the package, and the sample can be efficiently transferred to the microchip.
- the capillary phenomenon causes the package. It becomes easy to push up along the outer wall, and it is possible to avoid mixing the medium that interferes with analysis into the microchip channel. As a result, the analysis accuracy can be improved.
- the time for introducing samples to the microchip by the operator is greatly shortened, and sample loss is reduced. Prevention and cross-contamination can be prevented.
- the sample receiving tank and the reaction tank and the flow path connected between the sample receiving tank and the reaction tank, and the reaction of minute components contained in the sample are performed.
- a sample filling apparatus for filling a sample into a microchip In a sample filling apparatus for filling a sample into a microchip,
- a sample receiving chamber is filled with a sample in a sample chamber by mounting a package having a sample chamber filled with a sample in advance on a microchip.
- the sample chamber provided in the package has a convex shape, and the sample chamber is inserted into the sample receiving tank provided in the microchip so that the sample in the sample chamber is inserted into the sample chamber. Fill the receiving tank.
- the sample chamber is configured by a coating portion having an upper portion made of an elastic body, and the coating portion is bent by applying a pressure from a medium from above, so that the sample in the sample chamber is pushed out of the sample chamber.
- the sample chamber has a movable portion movable by applying a pressure from a medium from above, and the sample in the sample chamber is moved outside the sample chamber by moving the movable portion in the sample chamber. To extrude.
- the sample chamber has a broken portion at the bottom, and the broken portion is broken by an external physical force applied when the package is mounted on the microchip, and the sample in the sample chamber is Flows out into the sample receiving tank provided in the microchip.
- a protrusion is provided at the bottom of the sample chamber, and the protrusion breaks down the part to be destroyed by an external physical force applied when the package is mounted on the microchip. To do.
- a protrusion is provided on the upper surface of the sample receiving tank provided in the microphone mouth chip so as to contact the bottom of the sample chamber, and when the package is mounted on the microchip.
- the projecting portion destroys the to-be-destructed portion by an external physical force applied to.
- a gap is formed between the sample chamber and the sample receiving tank, and the sample in the sample chamber flows out due to destruction of the non-destructive portion.
- a part of the spilled sample is caused by capillary action. The medium mixed in the vicinity of the broken portion is pushed upward by flowing into the gap.
- the sample leaks to the outside when the sample in the sample chamber flows out into the sample receiving tank.
- a seal portion so as to seal the gap portion around the upper portion of the sample chamber
- the sample in the sample receiving tank flows out into the flow path without mixing of the medium by the seal portion.
- the sample flowing out into the flow path is guided to the reaction vessel and subjected to reaction and analysis of fine components contained in the sample.
- a space portion having a container shape for accumulating the medium is provided between the sample chamber and the sample receiving tank.
- a plurality of the sample chambers are provided to be connected to the package, and a plurality of the sample receiving tanks are provided to be connected to the microchip, and are provided to be connected to the package.
- the interval between the sample chambers coincided with the interval between the sample receiving tanks provided on the microchip.
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009532230A JP5532218B2 (ja) | 2007-09-10 | 2008-09-05 | 試料充填装置 |
US12/677,471 US8470266B2 (en) | 2007-09-10 | 2008-09-05 | Sample packing device |
US13/899,098 US8845980B2 (en) | 2007-09-10 | 2013-05-21 | Sample packing device |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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JP2007-234163 | 2007-09-10 | ||
JP2007234163 | 2007-09-10 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/677,471 A-371-Of-International US8470266B2 (en) | 2007-09-10 | 2008-09-05 | Sample packing device |
US13/899,098 Continuation US8845980B2 (en) | 2007-09-10 | 2013-05-21 | Sample packing device |
Publications (1)
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WO2009035062A1 true WO2009035062A1 (ja) | 2009-03-19 |
Family
ID=40452069
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/JP2008/066478 WO2009035062A1 (ja) | 2007-09-10 | 2008-09-05 | 試料充填装置 |
Country Status (3)
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---|---|
US (2) | US8470266B2 (ja) |
JP (1) | JP5532218B2 (ja) |
WO (1) | WO2009035062A1 (ja) |
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Also Published As
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US20130251603A1 (en) | 2013-09-26 |
US8470266B2 (en) | 2013-06-25 |
JPWO2009035062A1 (ja) | 2010-12-24 |
US20110002812A1 (en) | 2011-01-06 |
US8845980B2 (en) | 2014-09-30 |
JP5532218B2 (ja) | 2014-06-25 |
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