WO2009001868A1 - 幼若白血球の分析用試薬及び分析用試薬キット - Google Patents

幼若白血球の分析用試薬及び分析用試薬キット Download PDF

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Publication number
WO2009001868A1
WO2009001868A1 PCT/JP2008/061572 JP2008061572W WO2009001868A1 WO 2009001868 A1 WO2009001868 A1 WO 2009001868A1 JP 2008061572 W JP2008061572 W JP 2008061572W WO 2009001868 A1 WO2009001868 A1 WO 2009001868A1
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WIPO (PCT)
Prior art keywords
reagent
leukocyte
primitive
analysis
kit
Prior art date
Application number
PCT/JP2008/061572
Other languages
English (en)
French (fr)
Inventor
Yukiko Kataoka
Tomohiro Tsuji
Shinichiro Oguni
Ayumu Yoshida
Masaki Abe
Original Assignee
Sysmex Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sysmex Corporation filed Critical Sysmex Corporation
Priority to EP08777588.8A priority Critical patent/EP2166353B1/en
Priority to KR1020097025267A priority patent/KR101331332B1/ko
Priority to BRPI0811799A priority patent/BRPI0811799C1/pt
Priority to MX2009012997A priority patent/MX2009012997A/es
Priority to CN200880020635.0A priority patent/CN101680879B/zh
Priority to JP2009520620A priority patent/JP4981907B2/ja
Priority to ES08777588.8T priority patent/ES2684695T3/es
Priority to US12/666,774 priority patent/US8455209B2/en
Priority to PL08777588T priority patent/PL2166353T3/pl
Publication of WO2009001868A1 publication Critical patent/WO2009001868A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/100833—Simulative of a gaseous composition
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、赤血球及び成熟白血球の細胞膜に損傷を与える界面活性剤と、損傷した血球を収縮させる可溶化剤と、核酸染色色素とを含み、浸透圧が１０ｍＯｓｍ／ｋｇ以上１５０ｍＯｓｍ／ｋｇ未満である幼若白血球の分析用試薬を提供する。
PCT/JP2008/061572 2007-06-25 2008-06-25 幼若白血球の分析用試薬及び分析用試薬キット WO2009001868A1 (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EP08777588.8A EP2166353B1 (en) 2007-06-25 2008-06-25 Reagent and reagent kit for analysis of primitive leukocyte
KR1020097025267A KR101331332B1 (ko) 2007-06-25 2008-06-25 유약 백혈구 분석용 시약 및 분석용 시약 키트
BRPI0811799A BRPI0811799C1 (pt) 2007-06-25 2008-06-25 reagente para análise de leucócitos imaturos, kit de reagentes para análise de leucócitos imaturos e método para classificar leucócitos
MX2009012997A MX2009012997A (es) 2007-06-25 2008-06-25 Reactivo y equipo de reactivo para analisis de leucocito inmaduro.
CN200880020635.0A CN101680879B (zh) 2007-06-25 2008-06-25 幼稚白细胞的分析用试剂及分析用试剂盒
JP2009520620A JP4981907B2 (ja) 2007-06-25 2008-06-25 幼若白血球の分析用試薬及び分析用試薬キット
ES08777588.8T ES2684695T3 (es) 2007-06-25 2008-06-25 Reactivo y kit de reactivos para el análisis de leucocitos primitivos
US12/666,774 US8455209B2 (en) 2007-06-25 2008-06-25 Reagent and reagent kit for analysis of immature leukocyte
PL08777588T PL2166353T3 (pl) 2007-06-25 2008-06-25 Odczynnik i zestaw odczynników do analizy prymitywnego leukocytu

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007166609 2007-06-25
JP2007-166609 2007-06-25
JP2008084137 2008-03-27
JP2008-084137 2008-03-27

Publications (1)

Publication Number Publication Date
WO2009001868A1 true WO2009001868A1 (ja) 2008-12-31

Family

ID=40185680

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/061572 WO2009001868A1 (ja) 2007-06-25 2008-06-25 幼若白血球の分析用試薬及び分析用試薬キット

Country Status (12)

Country Link
US (1) US8455209B2 (ja)
EP (1) EP2166353B1 (ja)
JP (1) JP4981907B2 (ja)
KR (1) KR101331332B1 (ja)
CN (1) CN101680879B (ja)
BR (1) BRPI0811799C1 (ja)
ES (1) ES2684695T3 (ja)
HU (1) HUE040270T2 (ja)
MX (1) MX2009012997A (ja)
PL (1) PL2166353T3 (ja)
RU (1) RU2435164C2 (ja)
WO (1) WO2009001868A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014520251A (ja) * 2011-05-04 2014-08-21 アボット・ラボラトリーズ 白血球分析システムおよび方法
JP2014196958A (ja) * 2013-03-29 2014-10-16 シスメックス株式会社 血球分析装置および血球分析方法
US9778163B2 (en) 2011-05-04 2017-10-03 Abbott Laboratories Nucleated red blood cell analysis system and method
US9810618B2 (en) 2011-05-04 2017-11-07 Abbott Laboratories Basophil analysis system and method

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3104177B1 (en) * 2011-12-21 2020-11-25 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes
CN102618060A (zh) * 2012-03-17 2012-08-01 江南大学 一种不对称菁染料的制备及其用于牛血清白蛋白检测的方法
JP6383216B2 (ja) * 2014-08-08 2018-08-29 シスメックス株式会社 血液分析方法、血液分析装置およびプログラム
CN106687810B (zh) * 2014-12-31 2019-10-22 深圳迈瑞生物医疗电子股份有限公司 一种非诊断目的的有核红细胞报警方法、装置及流式细胞分析仪
EP3584565B1 (en) * 2017-02-17 2022-08-24 Shenzhen Mindray Bio-Medical Electronics Co., Ltd Red blood cell debris identification method and device and blood cell analyzer and analysis method
JP6903494B2 (ja) * 2017-06-09 2021-07-14 シスメックス株式会社 感染症を識別するための粒子分析方法
WO2022099659A1 (zh) * 2020-11-13 2022-05-19 大连理工大学 白细胞分类试剂、红细胞分析试剂、试剂盒以及分析方法

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JPH0599919A (ja) * 1991-04-22 1993-04-23 Hitachi Ltd 白血球分析方法
JPH06273413A (ja) * 1993-03-19 1994-09-30 Toa Medical Electronics Co Ltd 幼若細胞測定用試薬
JPH10206423A (ja) 1996-11-20 1998-08-07 Toa Medical Electronics Co Ltd 幼若白血球の分類計数法
JP2000162209A (ja) * 1998-11-27 2000-06-16 Sysmex Corp 赤芽球の分類計数用試薬及び分類計数方法
JP2002223791A (ja) * 2000-11-08 2002-08-13 Sysmex Corp 骨髄有核細胞の分類計数方法
JP2003329668A (ja) * 2002-05-16 2003-11-19 Sysmex Corp 骨髄液有核細胞自動分析方法
WO2004001408A1 (ja) * 2002-06-24 2003-12-31 Sysmex Corporation 白血球の分類計数方法
JP2006091024A (ja) * 2005-10-28 2006-04-06 Sysmex Corp 白血球分類計数方法及び白血球分類計数試薬キット
JP2006208401A (ja) * 2006-04-23 2006-08-10 Sysmex Corp 赤芽球の分類計数方法
JP2007166609A (ja) 2005-12-12 2007-06-28 Swatch Group Research & Development Ltd 広いダイナミック・レンジのイメージセンサを用いてイメージを得る方法

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US7049093B2 (en) 2000-11-08 2006-05-23 Sysmex Corporation Method of classifying and counting nucleated bone marrow cells
JP2002207036A (ja) 2001-01-10 2002-07-26 Sysmex Corp 白血球腫瘍細胞検出方法
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Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03252556A (ja) * 1990-03-01 1991-11-11 Toa Medical Electronics Co Ltd 白血球分類方法および試薬
JPH0599919A (ja) * 1991-04-22 1993-04-23 Hitachi Ltd 白血球分析方法
JPH06273413A (ja) * 1993-03-19 1994-09-30 Toa Medical Electronics Co Ltd 幼若細胞測定用試薬
JPH10206423A (ja) 1996-11-20 1998-08-07 Toa Medical Electronics Co Ltd 幼若白血球の分類計数法
JP2000162209A (ja) * 1998-11-27 2000-06-16 Sysmex Corp 赤芽球の分類計数用試薬及び分類計数方法
JP2002223791A (ja) * 2000-11-08 2002-08-13 Sysmex Corp 骨髄有核細胞の分類計数方法
JP2003329668A (ja) * 2002-05-16 2003-11-19 Sysmex Corp 骨髄液有核細胞自動分析方法
WO2004001408A1 (ja) * 2002-06-24 2003-12-31 Sysmex Corporation 白血球の分類計数方法
JP2006091024A (ja) * 2005-10-28 2006-04-06 Sysmex Corp 白血球分類計数方法及び白血球分類計数試薬キット
JP2007166609A (ja) 2005-12-12 2007-06-28 Swatch Group Research & Development Ltd 広いダイナミック・レンジのイメージセンサを用いてイメージを得る方法
JP2006208401A (ja) * 2006-04-23 2006-08-10 Sysmex Corp 赤芽球の分類計数方法

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014520251A (ja) * 2011-05-04 2014-08-21 アボット・ラボラトリーズ 白血球分析システムおよび方法
US9778162B2 (en) 2011-05-04 2017-10-03 Abbott Laboratories White blood cell analysis system and method
US9778163B2 (en) 2011-05-04 2017-10-03 Abbott Laboratories Nucleated red blood cell analysis system and method
US9810618B2 (en) 2011-05-04 2017-11-07 Abbott Laboratories Basophil analysis system and method
US10481073B2 (en) 2011-05-04 2019-11-19 Abbott Laboratories Basophil analysis system and method
US10481072B2 (en) 2011-05-04 2019-11-19 Abbott Laboratories Nucleated red blood cell analysis system and method
US10481071B2 (en) 2011-05-04 2019-11-19 Abbott Laboratories White blood cell analysis system and method
JP2014196958A (ja) * 2013-03-29 2014-10-16 シスメックス株式会社 血球分析装置および血球分析方法

Also Published As

Publication number Publication date
RU2010102038A (ru) 2011-07-27
EP2166353A1 (en) 2010-03-24
RU2435164C2 (ru) 2011-11-27
KR20100032853A (ko) 2010-03-26
JP4981907B2 (ja) 2012-07-25
HUE040270T2 (hu) 2019-02-28
ES2684695T3 (es) 2018-10-04
BRPI0811799C1 (pt) 2021-07-27
CN101680879B (zh) 2014-05-21
JPWO2009001868A1 (ja) 2010-08-26
KR101331332B1 (ko) 2013-11-20
PL2166353T3 (pl) 2019-01-31
US8455209B2 (en) 2013-06-04
BRPI0811799A2 (pt) 2014-10-21
BRPI0811799B8 (pt) 2021-06-15
EP2166353B1 (en) 2018-08-08
CN101680879A (zh) 2010-03-24
MX2009012997A (es) 2010-03-30
US20100184061A1 (en) 2010-07-22
EP2166353A4 (en) 2010-12-01
BRPI0811799B1 (pt) 2021-02-23

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