WO2008102602A1 - Ｂｌａｓｔｏｃｙｓｔ ｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法 - Google Patents

Ｂｌａｓｔｏｃｙｓｔ ｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法 Download PDF

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Publication number
WO2008102602A1
WO2008102602A1 PCT/JP2008/051129 JP2008051129W WO2008102602A1 WO 2008102602 A1 WO2008102602 A1 WO 2008102602A1 JP 2008051129 W JP2008051129 W JP 2008051129W WO 2008102602 A1 WO2008102602 A1 WO 2008102602A1
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WO
WIPO (PCT)
Prior art keywords
kidney
thymus
pancreas
hair
method utilizing
Prior art date
Application number
PCT/JP2008/051129
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English (en)
French (fr)
Inventor
Hiromitsu Nakauchi
Toshihiro Kobayashi
Younsu Lee
Joichi Usui
Original Assignee
The University Of Tokyo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by The University Of Tokyo filed Critical The University Of Tokyo
Priority to CN200880013001A priority Critical patent/CN101679950A/zh
Priority to JP2009500116A priority patent/JPWO2008102602A1/ja
Publication of WO2008102602A1 publication Critical patent/WO2008102602A1/ja
Priority to US12/583,559 priority patent/US20100122360A1/en
Priority to US15/375,982 priority patent/US20170280692A1/en
Priority to US16/356,570 priority patent/US20200008404A1/en
Priority to US17/497,356 priority patent/US20220338452A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/20—Animals treated with compounds which are neither proteins nor nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

本発明は、腎臓、すい臓、胸腺及び毛などの複数種の細胞からなる複雑な細胞構成を有する哺乳動物の臓器を、非ヒト動物の生体中で作成することを課題とする。本発明者らは、上述したキメラ動物・アッセイを、固形臓器の新規作製方法に応用した。より具体的には、上述したキメラ動物・アッセイを、成体腎臓の大部分へ分化する後腎間葉の機能異常により腎臓、すい臓、胸腺及び毛が欠損するモデルマウスを、胚盤胞補完作用（ｂｌａｓｔｏｃｙｓｔ　ｃｏｍｐｌｅｍｅｎｔａｔｉｏｎ）によりレスキューすることにより、新規に腎臓、すい臓、胸腺及び毛を製造することができることを示した。
PCT/JP2008/051129 2007-02-22 2008-01-25 Ｂｌａｓｔｏｃｙｓｔ ｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法 WO2008102602A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN200880013001A CN101679950A (zh) 2007-02-22 2008-01-25 利用胚泡互补的器官再生法
JP2009500116A JPWO2008102602A1 (ja) 2007-02-22 2008-01-25 Ｂｌａｓｔｏｃｙｓｔｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法
US12/583,559 US20100122360A1 (en) 2007-02-22 2009-08-21 Organ regeneration method utilizing blastocyst complementation
US15/375,982 US20170280692A1 (en) 2007-02-22 2016-12-12 Organ regeneration method utilizing blastocyst complementation
US16/356,570 US20200008404A1 (en) 2007-02-22 2019-03-18 Organ regeneration method utilizing blastocyst complementation
US17/497,356 US20220338452A1 (en) 2007-02-22 2021-10-08 Organ regeneration method utilizing blastocyst complementation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-042041 2007-02-22
JP2007042041 2007-02-22
JP2007-311786 2007-11-30
JP2007311786 2007-11-30

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/583,559 Continuation-In-Part US20100122360A1 (en) 2007-02-22 2009-08-21 Organ regeneration method utilizing blastocyst complementation

Publications (1)

Publication Number Publication Date
WO2008102602A1 true WO2008102602A1 (ja) 2008-08-28

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Application Number Title Priority Date Filing Date
PCT/JP2008/051129 WO2008102602A1 (ja) 2007-02-22 2008-01-25 Ｂｌａｓｔｏｃｙｓｔ ｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法

Country Status (4)

Country Link
US (4) US20100122360A1 (ja)
JP (6) JPWO2008102602A1 (ja)
CN (1) CN101679950A (ja)
WO (1) WO2008102602A1 (ja)

Cited By (10)

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WO2009104794A1 (ja) * 2008-02-22 2009-08-27 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
WO2010021390A1 (ja) * 2008-08-22 2010-02-25 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴ ＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
US20100122360A1 (en) * 2007-02-22 2010-05-13 The University Of Tokyo Organ regeneration method utilizing blastocyst complementation
WO2010087459A1 (ja) * 2009-01-30 2010-08-05 国立大学法人東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
WO2014119627A1 (ja) 2013-01-29 2014-08-07 国立大学法人 東京大学 キメラ動物の作製方法
JP2018523999A (ja) * 2015-06-30 2018-08-30 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ヒト化心筋
CN108660161A (zh) * 2017-03-31 2018-10-16 中国科学院上海生命科学研究院 基于CRISPR/Cas9技术的制备无嵌合基因敲除动物的方法
US10246679B2 (en) 2015-03-02 2019-04-02 The University Of Tokyo Method for producing pluripotent stem cell having improved chimera forming ability from primed pluripotent stem cell, and composition and combination therefor
US10874092B2 (en) 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
US11673928B2 (en) 2015-03-03 2023-06-13 Regents Of The University Of Minnesota Genetically modified pig cells with an inactivated Etv2 gene

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CN107734965A (zh) 2015-04-08 2018-02-23 全国农业协同组合连合会 生产来自不同个体的配子的非人大型哺乳动物或鱼类的制作方法
CN105518137B (zh) * 2015-06-11 2021-04-30 深圳市第二人民医院 CRISPR-Cas9特异性敲除猪SALL1基因的方法及用于特异性靶向SALL1基因的sgRNA
AU2016344152A1 (en) * 2015-10-27 2018-05-24 Recombinetics, Inc. Compositions and methods for chimeric embryo-assisted organ production
EP3480298B1 (en) * 2016-06-29 2024-09-25 Takashi Yokoo Kidney production method
US11559605B2 (en) 2016-06-29 2023-01-24 Takashi Yokoo Kidney production method
JP6830674B2 (ja) 2017-01-25 2021-02-17 国立大学法人 東京大学 キメラ動物における出生後の炎症の発見とその治療
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Cited By (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100122360A1 (en) * 2007-02-22 2010-05-13 The University Of Tokyo Organ regeneration method utilizing blastocyst complementation
JPWO2009104794A1 (ja) * 2008-02-22 2011-06-23 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
WO2009104794A1 (ja) * 2008-02-22 2009-08-27 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
JP5688788B2 (ja) * 2008-02-22 2015-03-25 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
JP2017018133A (ja) * 2008-08-22 2017-01-26 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴ ＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
JP5688800B2 (ja) * 2008-08-22 2015-03-25 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
JPWO2010021390A1 (ja) * 2008-08-22 2012-01-26 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
GB2475656A (en) * 2008-08-22 2011-05-25 Univ Tokyo Organ regeneration method utilizing iPS cell and blastocyst complementation
GB2475656B (en) * 2008-08-22 2013-04-24 Univ Tokyo Organ regeneration method utilizing ips cell and blastocyst complementation
JP2019030310A (ja) * 2008-08-22 2019-02-28 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴ ＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
WO2010021390A1 (ja) * 2008-08-22 2010-02-25 国立大学法人 東京大学 ｉＰＳ細胞とＢＬＡＳＴＯＣＹＳＴ ＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
US20140338008A1 (en) * 2008-08-22 2014-11-13 The University Of Tokyo ORGAN REGENERATION METHOD UTILIZING iPS CELL AND BLASTOCYST COMPLEMENTATION
JP2014230552A (ja) * 2008-08-22 2014-12-11 国立大学法人 東京大学 ｉＰＳ細胞などの多能性細胞とＢＬＡＳＴＯＣＹＳＴＣＯＭＰＬＥＭＥＮＴＡＴＩＯＮを利用した臓器再生法
JP2020043864A (ja) * 2009-01-30 2020-03-26 国立大学法人 東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
WO2010087459A1 (ja) * 2009-01-30 2010-08-05 国立大学法人東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
JP2016154555A (ja) * 2009-01-30 2016-09-01 国立大学法人 東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
JPWO2010087459A1 (ja) * 2009-01-30 2012-08-02 国立大学法人 東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
JP2014113160A (ja) * 2009-01-30 2014-06-26 Univ Of Tokyo 幹細胞を用いた異種間胚胞キメラ動物の作製法
JP2018102303A (ja) * 2009-01-30 2018-07-05 国立大学法人 東京大学 幹細胞を用いた異種間胚胞キメラ動物の作製法
JP2018046833A (ja) * 2013-01-29 2018-03-29 国立大学法人 東京大学 キメラ動物の作製方法
JP2018082716A (ja) * 2013-01-29 2018-05-31 国立大学法人 東京大学 キメラ動物の作製方法
JP6279141B1 (ja) * 2013-01-29 2018-02-14 国立大学法人 東京大学 キメラ動物の作製方法
US11844336B2 (en) 2013-01-29 2023-12-19 The University Of Tokyo Method for producing chimeric animal
JPWO2014119627A1 (ja) * 2013-01-29 2017-01-26 国立大学法人 東京大学 キメラ動物の作製方法
WO2014119627A1 (ja) 2013-01-29 2014-08-07 国立大学法人 東京大学 キメラ動物の作製方法
US10645912B2 (en) 2013-01-29 2020-05-12 The University Of Tokyo Method for producing chimeric animal
US10246679B2 (en) 2015-03-02 2019-04-02 The University Of Tokyo Method for producing pluripotent stem cell having improved chimera forming ability from primed pluripotent stem cell, and composition and combination therefor
US11673928B2 (en) 2015-03-03 2023-06-13 Regents Of The University Of Minnesota Genetically modified pig cells with an inactivated Etv2 gene
US10897880B2 (en) 2015-06-30 2021-01-26 Regents Of The University Of Minnesota Humanized heart muscle
US10874092B2 (en) 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
JP2018523999A (ja) * 2015-06-30 2018-08-30 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ヒト化心筋
US12089574B2 (en) 2015-06-30 2024-09-17 Regents Of The University Of Minnesota Humanized skeletal muscle
CN108660161B (zh) * 2017-03-31 2023-05-09 中国科学院脑科学与智能技术卓越创新中心 基于CRISPR/Cas9技术的制备无嵌合基因敲除动物的方法
CN108660161A (zh) * 2017-03-31 2018-10-16 中国科学院上海生命科学研究院 基于CRISPR/Cas9技术的制备无嵌合基因敲除动物的方法

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US20220338452A1 (en) 2022-10-27
JP2014121329A (ja) 2014-07-03
CN101679950A (zh) 2010-03-24
US20100122360A1 (en) 2010-05-13
JP2015061525A (ja) 2015-04-02
US20170280692A1 (en) 2017-10-05
JP2018138048A (ja) 2018-09-06
JP2016195605A (ja) 2016-11-24
US20200008404A1 (en) 2020-01-09
JP5725587B2 (ja) 2015-05-27
JPWO2008102602A1 (ja) 2010-05-27
JP2020078327A (ja) 2020-05-28

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