WO2008081922A1 - TRACP5bの製造方法 - Google Patents

TRACP5bの製造方法 Download PDF

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Publication number
WO2008081922A1
WO2008081922A1 PCT/JP2007/075263 JP2007075263W WO2008081922A1 WO 2008081922 A1 WO2008081922 A1 WO 2008081922A1 JP 2007075263 W JP2007075263 W JP 2007075263W WO 2008081922 A1 WO2008081922 A1 WO 2008081922A1
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WO
WIPO (PCT)
Prior art keywords
tracp5b
silkworm
dna encoding
gland
silk
Prior art date
Application number
PCT/JP2007/075263
Other languages
English (en)
French (fr)
Inventor
Iwao Kiyokawa
Tatsuya Ohashi
Toshihide Miura
Katsuhiro Katayama
Toshiki Tamura
Isao Kobayashi
Hideki Sezutsu
Original Assignee
Nitto Boseki Co., Ltd
National Institute Of Agrobiological Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Boseki Co., Ltd, National Institute Of Agrobiological Sciences filed Critical Nitto Boseki Co., Ltd
Priority to CN2007800518659A priority Critical patent/CN101617041B/zh
Priority to JP2008552177A priority patent/JP5177431B2/ja
Priority to KR1020097015501A priority patent/KR101443937B1/ko
Priority to US12/521,701 priority patent/US8426674B2/en
Priority to EP07860466.7A priority patent/EP2119772B1/en
Publication of WO2008081922A1 publication Critical patent/WO2008081922A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　（ｉ）絹糸腺特異的に発現するタンパク質をコードするDNA のプロモーターの下流に、機能的に結合した転写制御因子をコードするDNA、及び（ｉｉ）該転写制御因子の標的プロモーターの下流に、機能的に結合した酒石酸抵抗性酸性ホスファターゼ5b(TRACP5b)をコードするDNA、を有するカイコを作成した。その結果、該カイコから、活性を有するTRACP5b が産生されることがわかった。このことは、該カイコの絹糸腺から産生されたTRACP5 は、絹糸腺で骨吸収部位におけるプロセッシングと同様なプロセッシングを受けることを意味する。
PCT/JP2007/075263 2006-12-28 2007-12-28 TRACP5bの製造方法 WO2008081922A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN2007800518659A CN101617041B (zh) 2006-12-28 2007-12-28 TRACP5b的生产方法
JP2008552177A JP5177431B2 (ja) 2006-12-28 2007-12-28 ＴＲＡＣＰ５ｂの製造方法
KR1020097015501A KR101443937B1 (ko) 2006-12-28 2007-12-28 ＴＲＡＣＰ５ｂ의 제조방법
US12/521,701 US8426674B2 (en) 2006-12-28 2007-12-28 Method for production of TRACP5b
EP07860466.7A EP2119772B1 (en) 2006-12-28 2007-12-28 METHOD FOR PRODUCTION OF TRACP5b

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006-353542 2006-12-28
JP2006353542 2006-12-28

Publications (1)

Publication Number Publication Date
WO2008081922A1 true WO2008081922A1 (ja) 2008-07-10

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ID=39588602

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/075263 WO2008081922A1 (ja) 2006-12-28 2007-12-28 TRACP5bの製造方法

Country Status (6)

Country Link
US (1) US8426674B2 (ja)
EP (1) EP2119772B1 (ja)
JP (1) JP5177431B2 (ja)
KR (1) KR101443937B1 (ja)
CN (1) CN101617041B (ja)
WO (1) WO2008081922A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010047293A1 (ja) * 2008-10-20 2010-04-29 独立行政法人農業生物資源研究所 外来遺伝子発現カイコ繭の製糸方法及びそれによる製品

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107619836B (zh) * 2017-09-30 2020-10-09 西南大学 一种降低吐丝期活性20e浓度改变丝蛹营养分配比例增加蚕茧产量的系统及应用和方法

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JP2002510050A (ja) 1998-04-01 2002-04-02 ハーリーン、ジュッシ 骨吸収速度を測定する方法
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US6994966B2 (en) 2000-02-17 2006-02-07 Glycominds Ltd. Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof
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EP1359161B1 (en) * 2002-04-30 2010-08-25 Nitto Boseki Co., Ltd. Hybridoma capable of producing a monoclonal antibody specific to tartrate-resistant acid phospatase 5b (TRACP 5b)
JP2004135528A (ja) 2002-10-16 2004-05-13 National Institute Of Agrobiological Sciences カイコを利用したタンパク質の製造方法
JP2005095063A (ja) * 2003-09-25 2005-04-14 National Institute Of Agrobiological Sciences カイコ絹糸腺細胞から絹糸腺内腔への移行活性を有するタンパク質からシグナル領域が除去されたタンパク質の製造方法
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WO2004059320A1 (ja) 2002-12-26 2004-07-15 Nitto Boseki Co., Ltd. 免疫測定法およびそれに用いるキット
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010047293A1 (ja) * 2008-10-20 2010-04-29 独立行政法人農業生物資源研究所 外来遺伝子発現カイコ繭の製糸方法及びそれによる製品

Also Published As

Publication number Publication date
CN101617041A (zh) 2009-12-30
KR20090094391A (ko) 2009-09-04
US20110239313A1 (en) 2011-09-29
CN101617041B (zh) 2011-08-10
JP5177431B2 (ja) 2013-04-03
US8426674B2 (en) 2013-04-23
EP2119772B1 (en) 2013-11-20
EP2119772A1 (en) 2009-11-18
KR101443937B1 (ko) 2014-09-23
JPWO2008081922A1 (ja) 2010-04-30
EP2119772A4 (en) 2010-11-17

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