WO2012047037A3 - 배아줄기세포 유래 심근세포 및 이를 유효성분으로 포함하는 세포치료제 - Google Patents

배아줄기세포 유래 심근세포 및 이를 유효성분으로 포함하는 세포치료제 Download PDF

Info

Publication number
WO2012047037A3
WO2012047037A3 PCT/KR2011/007408 KR2011007408W WO2012047037A3 WO 2012047037 A3 WO2012047037 A3 WO 2012047037A3 KR 2011007408 W KR2011007408 W KR 2011007408W WO 2012047037 A3 WO2012047037 A3 WO 2012047037A3
Authority
WO
WIPO (PCT)
Prior art keywords
therapy product
cardiomyocyte
cell therapy
embryonic stem
cardiomyocytes
Prior art date
Application number
PCT/KR2011/007408
Other languages
English (en)
French (fr)
Other versions
WO2012047037A2 (ko
Inventor
정형민
문성환
Original Assignee
(주)차바이오앤디오스텍
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)차바이오앤디오스텍 filed Critical (주)차바이오앤디오스텍
Priority to CN2011800582933A priority Critical patent/CN103237887A/zh
Publication of WO2012047037A2 publication Critical patent/WO2012047037A2/ko
Publication of WO2012047037A3 publication Critical patent/WO2012047037A3/ko
Priority to US13/907,967 priority patent/US9969978B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cardiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Vascular Medicine (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

본 발명은 배아줄기세포 유래 심근세포를 제조하는 방법, 상기 방법으로 제조된 심근세포, 상기 심근세포로부터 심근세포 응집체를 제조하는 방법, 상기 방법으로 제조된 심근세포 응집체, 상기 심근세포 응집체를 유효성분으로 포함하는 심장질환 치료용 세포치료제, 상기 세포치료제를 이용한 심장질환의 치료방법 및 상기 세포치료제의 제조를 위한 심근세포 또는 심근세포 응집체의 용도에 관한 것이다. 본 발명의 심근세포의 제조방법을 이용하면, 배아줄기세포로부터 분화된 심근세포를 용이하게 정제할 수 있고, 상기 정제된 심근세포로부터 심근세포 응집체를 제조하여 심장질환의 치료에 사용될 수 있는 세포치료제로서 사용할 수 있으므로, 각종 심장질환의 예방 또는 치료용 제제의 개발에 널리 활용될 수 있을 것이다.
PCT/KR2011/007408 2010-10-06 2011-10-06 배아줄기세포 유래 심근세포 및 이를 유효성분으로 포함하는 세포치료제 WO2012047037A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2011800582933A CN103237887A (zh) 2010-10-06 2011-10-06 胚胎干细胞来源的心肌细胞以及包含所述心肌细胞作为活性成分的细胞治疗剂
US13/907,967 US9969978B2 (en) 2010-10-06 2013-06-02 Method for producing cardiomyocytes from human or mouse embryonic stem cells in a medium consisting of a serum-free medium and N2 supplement

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0097543 2010-10-06
KR1020100097543A KR101364965B1 (ko) 2010-10-06 2010-10-06 인간 배아줄기세포 유래 심근세포의 배양 및 정제 방법

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/907,967 Continuation US9969978B2 (en) 2010-10-06 2013-06-02 Method for producing cardiomyocytes from human or mouse embryonic stem cells in a medium consisting of a serum-free medium and N2 supplement

Publications (2)

Publication Number Publication Date
WO2012047037A2 WO2012047037A2 (ko) 2012-04-12
WO2012047037A3 true WO2012047037A3 (ko) 2012-06-07

Family

ID=45928234

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/007408 WO2012047037A2 (ko) 2010-10-06 2011-10-06 배아줄기세포 유래 심근세포 및 이를 유효성분으로 포함하는 세포치료제

Country Status (4)

Country Link
US (1) US9969978B2 (ko)
KR (1) KR101364965B1 (ko)
CN (1) CN103237887A (ko)
WO (1) WO2012047037A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104988116A (zh) * 2015-08-07 2015-10-21 上海交通大学医学院 一种化学品胚胎毒性预测模型及其建立方法
CN107153115A (zh) * 2017-05-31 2017-09-12 上海众择生物科技有限公司 一种用于混合淋巴细胞共培养试验检测的方法和试剂盒
EP3569693A4 (en) * 2017-06-05 2020-11-18 Terumo Kabushiki Kaisha METHOD OF PREPARING A CELL CULTURE
KR101994035B1 (ko) * 2018-02-14 2019-06-27 (주) 넥셀 세포 분화 및 성숙을 통한 인간 전분화능 줄기세포 유래 기능성 심근세포의 제조 방법
KR102188256B1 (ko) * 2019-12-02 2020-12-08 주식회사 티앤알바이오팹 동결보관 및 저산소 조건에서의 생존율을 향상시키는 심근세포 응집체 제작 기술
CN115521905A (zh) * 2021-06-24 2022-12-27 卓越细胞工程(香港)有限公司 干细胞分化为心肌细胞的培养方法和培养基

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030031651A1 (en) * 2001-04-13 2003-02-13 Lee Ike W. Methods and reagents for cell transplantation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ537245A (en) * 2002-07-26 2010-04-30 Wisconsin Alumni Res Found Method of testing cardiomyocytes derived from human embryonic stem cells formed in embryoid bodies differentiated into all of the major cardiac muscle cell types, including ventricular, atrial, and nodal cells
AU2005224670B2 (en) 2004-03-19 2010-11-11 Asterias Biotherapeutics, Inc. Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
SG169359A1 (en) * 2006-01-31 2011-03-30 Univ Keio A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030031651A1 (en) * 2001-04-13 2003-02-13 Lee Ike W. Methods and reagents for cell transplantation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HUBER ET AL.: "Identification and selection of cardiomyocytes during human embryonic stem cell differentiation", THE FASEB JOURNAL, vol. 21, August 2007 (2007-08-01), pages 2551 - 2563, XP008132356, DOI: doi:10.1096/fj.05-5711com *
ULLOA-MONTOYA ET AL.: "Culture systems for pluripotent stem cell", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 100, no. 1, 2005, pages 12 - 27 *
YAO ET AL.: "Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions", PNAS, vol. 103, no. 18, 2 May 2006 (2006-05-02), pages 6907 - 6912, XP002463153, DOI: doi:10.1073/pnas.0602280103 *

Also Published As

Publication number Publication date
WO2012047037A2 (ko) 2012-04-12
KR20120035801A (ko) 2012-04-16
KR101364965B1 (ko) 2014-02-18
US9969978B2 (en) 2018-05-15
CN103237887A (zh) 2013-08-07
US20130251691A1 (en) 2013-09-26

Similar Documents

Publication Publication Date Title
WO2013049726A3 (en) Processes for making compounds useful as inhibitors of atr kinase
MX347917B (es) Compuestos a base de imidazo[1,2-b]piridazina, composiciones que los comprenden, y metodos para su uso.
WO2016130920A3 (en) Lrrk2 inhibitors and methods of making and using the same
WO2013151668A3 (en) Modified polynucleotides for the production of secreted proteins
WO2012168430A3 (en) Polypeptides
WO2012047037A3 (ko) 배아줄기세포 유래 심근세포 및 이를 유효성분으로 포함하는 세포치료제
WO2012017324A3 (en) Combination pharmaceutical composition and methods of treating diseases or conditions associated with neurodegenerative diseases
PH12014501967A1 (en) Benzodioxanes in combination with other actives for inhibiting leukotriene production
WO2013040286A3 (en) Pharmaceutical compositions
MX2013000958A (es) Fabricación del inhibidor-inter-alfa (iaip) a partir de plasma.
WO2012025944A3 (en) Sitagliptin, salts and polymorphs thereof
NZ703797A (en) 4-methyl-2,3,5,9,9b-pentaaza-cyclopenta[a]naphthalenes
WO2011006143A3 (en) Fused imidazoles and compositions comprising them for the treatment of parasitic diseases, such as e.g. malaria
WO2010102778A3 (de) Substituierte 3-aminoisoxazolopyridine als kcnq2/3 modulatoren
WO2014178078A3 (en) Novel cloning, expression & purification method for the preparation of ranibizumab
MX2013012233A (es) 4-aril-n-fenil-1,3,5-triazin-2-aminas sustituidas.
WO2012017323A3 (en) Pharmaceutical composition and methods of treating and preventing the diseases caused by hiv or associated with hiv
PH12014501195A1 (en) Novel 2h-indazoles as ep2, receptor antagonists
WO2012010978A3 (en) A method of treating alzheimer's disease
WO2011132051A3 (en) Tricycle compounds as phosphodiesterase-10 inhibitors
UA110128C2 (uk) Індолкарбоксаміди та бензімідазолкарбоксаміди як інсектициди та акарициди
WO2012136898A3 (en) Novel cutinases, their production and uses
WO2012088222A3 (en) Methods and compositions for producing active vitamin k-dependent proteins
WO2011009532A3 (de) 17-hydroxy-17-pentafluorethyl-estra-4,9(10)-dien-11-benzyliden-derivate, verfahren zu deren herstellung und deren verwendung zur behandlung von krankheiten
MX2018004524A (es) Derivados de eter ciclico de pirazolo[1,5-a]pirimidin-3-carboxiami da.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11830922

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11830922

Country of ref document: EP

Kind code of ref document: A2