WO2007129674A1

WO2007129674A1 - Fermented product composition, cosmetic composition and method of producing the same

Info

Publication number: WO2007129674A1
Application number: PCT/JP2007/059398
Authority: WO
Inventors: Toshitaka Okada; Kenji Fujisawa; Hiroko Takahashi
Original assignee: Toyo Hakko Co., Ltd.
Priority date: 2006-05-02
Filing date: 2007-05-02
Publication date: 2007-11-15
Also published as: JPWO2007129674A1; CN101431980B; CN101431980A; CN102225045A; KR101397976B1; JP4945556B2; JP2011173921A; JP4945692B2; HK1130182A1; CN102225045B; KR20090007726A

Abstract

A fermented product composition and a cosmetic composition comprising a fermented product, which is obtained by fermenting peptides originating in rice bran and beans by using Bacillus subtilis, and at least one member selected from peptides and vitamin A analogs. A production method comprising a mixing step wherein a fermented product, which is obtained by fermenting peptides originating in rice bran and beans by using Bacillus subtilis, is mixed with at least one member selected from peptides and vitamin A analogs.

Description

Specification

Fermented product composition, cosmetic composition and method for producing them

Technical field

[0001] The present invention relates to a fermented product composition, a cosmetic composition, and a method for producing them. More specifically, the present invention relates to a fermented product composition excellent in collagen synthesizing action and the like with high safety. The present invention also relates to a cosmetic composition comprising the fermented product composition. Furthermore, this invention relates to the manufacturing method of the said fermented material composition and cosmetics composition.

Background art

[0002] Conventionally, cosmetics such as external preparations for skin containing fermented products obtained by fermenting rice bran and the like are known. For example, Patent Document 1 discloses a cosmetic composition containing a fermented rice cake and a cosmetic using the same. In Patent Document 1, it is described that this cosmetic product is excellent in moisture retention and is comfortable and less irritating when applied to the skin. Patent Document 2 discloses a method for producing a raw material for external preparation for skin obtained by fermenting rice bran and soybean peptide with Bacillus subtilis and then purifying it. In Patent Document 2, the raw material for external preparation for skin is described as having the ability S to enhance the activity of the skin, which is highly safe.

Patent Document 1: Japanese Patent Application Laid-Open No. 2004-262829

Patent Document 2: JP-A-8-104647

Disclosure of the invention

Problems to be solved by the invention

[0004] The effects of blending the fermented products described in Patent Documents 1 and 2 into cosmetics such as external preparations for skin are many. For example, cosmetics such as an external preparation for skin containing this fermented product can suppress wrinkles, blemishes, sagging and the like, and can prevent dullness, redness and rough skin. In addition, cosmetics such as external preparations for skin containing this fermented product can provide effects such as wound healing and improvement of symptoms such as burns.

[0005] An object of the present invention is to provide a fermented product composition excellent in collagen synthesizing action and the like with high safety. The present invention also provides a cosmetic composition comprising the fermented composition. The purpose is to do. Furthermore, an object of this invention is to provide the manufacturing method of the said fermented material composition and cosmetics composition.

Means for solving the problem

[0006] The present invention is as follows.

[1] A fermented product composition comprising a fermented product obtained by fermenting rice bran strength and a bean-derived peptide with Bacillus subtilis, and at least one of peptides and vitamins A

[2] The fermented product composition according to the above [1], wherein the peptide is at least one of a peptide obtained by hydrolyzing soybean protein and silk fiber mouth-in.

[3] A cosmetic composition comprising the fermented product composition described in [1] above.

[4] A fermented product composition comprising a mixing step of mixing fermented material obtained by fermenting rice bran and bean-derived peptides with Bacillus subtilis and at least one of peptide and vitamin A Manufacturing method.

[5] A mixing step of mixing a fermented product obtained by fermenting rice bran and bean-derived peptides with Bacillus subtilis and at least one of peptides and vitamin A, and a fermented product obtained in the mixing step And a step of formulating the composition to obtain a cosmetic composition. A method for producing a cosmetic composition, comprising:

The invention's effect

[0007] The fermented product composition of the present invention uses a natural product as a raw material. Therefore, the fermented product composition of the present invention has high safety and excellent collagen synthesis action and cell growth action.

Further, when the peptide is at least one of a peptide obtained by hydrolyzing soy protein and synolephifiproin, a fermented product composition that is more easily available and has sufficient collagen synthesis properties, etc. it can.

The cosmetic composition of the present invention has an excellent collagen synthesis action and cell proliferation action by having the above-described configuration.

According to the method for producing a fermented product composition and a cosmetic composition of the present invention, the fermented product composition and the cosmetic composition can be produced by fermentation using Bacillus subtilis which is easily available. Therefore, it has the above-mentioned excellent action The fermented product composition of the present invention can be produced safely and easily with a small production facility.

BEST MODE FOR CARRYING OUT THE INVENTION

[0008] Hereinafter, the present invention will be described in detail.

(1) Fermented product composition

The fermented product composition of the present invention contains a fermented product obtained by fermenting rice bran and a bean-derived peptide with Bacillus subtilis, and at least one of a peptide and vitamin A.

[0009] Examples of the "rice bran" include rice bran, defatted rice bran, rice germ, and defatted rice germ. These may be used alone or in combination of two or more. As the rice bran, various types of rice bran can be used without being limited to commercially available rice bran.

[0010] The above-mentioned "bean-derived peptide" is not particularly limited in its specific type and structure as long as it is a peptide contained in beans or a peptide derived from proteins contained in beans. As the “bean-derived peptide”, various kinds of bean-derived peptides can be used without being limited to commercially available bean-derived peptides. Examples of the bean-derived peptide include soybean peptide, red bean peptide, endo peptide and broad bean peptide. As the bean-derived peptide, soybean peptide is preferable. The legume-derived peptides may be used alone or in combination of two or more.

[0011] There is no particular limitation on the method for obtaining the bean-derived peptide. For example, the above-mentioned peptides derived from beans are made of beans (for example, one or more of soybeans, red beans, endu and broad beans) as solvents (water, hot water, organic solvents such as alcohol (ethanol etc.) or water-one organic solvents. It can be obtained by extraction with a mixed solvent. In this process, fractionation and purification can be performed as necessary. Furthermore, the obtained legume-derived peptide or legume protein can be further fragmented by subjecting it to a hydrolysis treatment with protease, acid or alkali. Furthermore, the above-mentioned bean-derived peptide is an artificially synthesized peptide obtained by chemically peptide-binding amino acids or by a known genetic engineering technique. [0012] There is no particular limitation on the form of the legume-derived peptide. The bean-derived peptide can be made into a solid (eg, a powdery product or a granular product) solidified by drying or the like after the above extraction. Moreover, the said extract itself can be used as said bean-derived peptide. That is, in the present invention, an extract containing a bean-derived peptide (bean extract, particularly soybean extract) can be used as the bean-derived peptide. For example, in the present invention, as the above-mentioned bean-derived peptide, beans (for example, one or more of soybeans, red beans, endu, and broad beans) are used as solvents (water, hot water, alcohol (ethanol, etc.) organic solvents. Alternatively, an extract obtained by extraction with a water-organic solvent mixed solvent) can be used. In the present invention, an extract (soybean extract) containing soybean peptide is particularly preferably used.

[0013] There are no particular limitations on the extraction method and extraction conditions for obtaining an extract containing the bean-derived peptide. For example, beans that are extraction raw materials may be unground or pulverized. Further, as long as the quality of the extract can be maintained, pretreatment such as impurity removal may be performed. Examples of the extraction solvent include water or hot water, alcohols such as methanol and ethanol, esters such as ethyl acetate, organic solvents such as n-xane, mixed solvents of these organic solvents and water or hot water, and the like. Can be used. As the extraction solvent, water or hot water, alcohol (such as methanol and ethanol), and a mixed solvent of water or hot water and alcohol are preferable. The temperature of the hot water is usually 40 to 100 ° C, preferably 50 80 ° C, more preferably 50 70 ° C. In addition, the pH of the extraction solvent during extraction is usually 37, preferably 46, and more preferably 45. It is preferable to adjust the pH within the above range since the stability of various components contained in the extraction raw material can be maintained. The extraction temperature is not particularly limited, but normal temperature or heat extraction is preferable. In the case of heat extraction, the heating temperature is usually 40 to 100 ° C, preferably 5080 ° C, more preferably 5070C. It is preferable because the extraction can be performed efficiently by setting the heating temperature within the effective range.

[0014] There is no particular limitation on the ratio between the above-mentioned rice force and the above-mentioned bean-derived peptide. Usually, when the bean-derived peptide is 100 parts by mass, the ratio of the rice bran is 120 parts by mass, preferably 520 parts by mass, more preferably 8 to 18 parts by mass. The above rice bran is in the above range If so, a fermented product having a sufficient collagen synthesis action and the like can be obtained.

[0015] The above-mentioned "Bacillus subtilis" [Bacillus subtilis] is a typical kind of aerobic spore-forming bacteria. The type of Bacillus subtilis is not particularly limited as long as safety is guaranteed. As the Bacillus subtilis, for example, Bacillus natto can be used. As the Bacillus subtilis, Bacillus natto that is easily available and inexpensive is preferable. As the Bacillus subtilis, commercially available general Bacillus subtilis can be used. As long as a fermented product having a collagen synthesizing action or the like is obtained, the Bacillus subtilis may be produced naturally or by a chemical substance such as nitrosoguanidine, X-rays, ultraviolet rays, etc. Mutants with altered chemistry can also be used.

[0016] The "fermented product" is obtained by fermenting the rice bran and the bean-derived peptide with Bacillus subtilis. The fermented product is usually obtained by ingesting Bacillus subtilis into a medium containing the rice bran and the beans-derived peptide and fermenting and culturing under appropriate conditions. The fermentation culture conditions for obtaining the above “fermented product” are not particularly limited. Fermentation culture is usually performed by aeration and agitation. The medium is not particularly limited as long as the Bacillus subtilis can grow. The medium is usually a liquid medium, but it cannot be a solid medium. The pH of the medium (especially during fermentation) is usually 5.5 to 8.5, preferably 6.0 to 8.0, and more preferably 6.5 to 7.5. If this pH is within the above range, it is preferable because the stability of various components contained in the extraction raw material can be maintained. Further, the culture temperature is not particularly limited as long as fermentation is performed. The culture temperature is usually 15 to 50 ° C, preferably 20 to 45 ° C, more preferably 30 to 45 ° C, and more preferably 35 to 43 ° C. In addition, various nutrients such as sugar and acid and alkali for pH adjustment may be added to the medium. Further, the fermentation may be mixed fermentation or continuous fermentation.

[0017] The form of the fermented product is not particularly limited. The fermented product may be a fermented liquid obtained by fermentation culture or a liquid obtained by filtering the fermented liquid. In addition, the fermented product is sterilized or pH-adjusted as necessary with respect to the fermented liquid, or after-treatment such as deodorization / decolorization using an ion exchange resin, an activated charcoal column or a dialysis membrane. Fermented broth may be used. Further, the fermented product may be a concentrated solution or a paste-like product obtained by concentrating the fermented solution. That In addition, the fermented product may be a solid product or a powdered product obtained by removing the solvent from the fermented solution by a known method such as freeze drying. Further, the fermented product is a solution or dispersion obtained by adding the fermented liquid or the solid and powdered materials to water or an organic solvent such as ethanol, propylene glycol and 1,3-butylene glycol, or a mixed solvent thereof. You can also use it as a liquid.

[0018] The "peptide" is a polypeptide in which amino acids are bound by peptide bonds. The amino acid may be a force D-amino acid, which is usually an L-amino acid, or a mixture of both. That is, the amino acids constituting the peptide are L-amino acids, D-amino acids, and a mixture of both, or may be shifted.

[0019] The type, origin, and structure of the peptide are not particularly limited. For example, the peptide may be a plant-derived peptide contained in a plant or an animal-derived peptide contained in an animal. Moreover, the structure of the peptide may include not only a linear structure but also a disulfide bond. Specific examples of the peptide include, for example, peptides obtained by hydrolyzing soy protein (proteins contained in soybean) and silk peptides such as silk-five phine (the peptides contained in silk and the proteins contained in silk are hydrolyzed. Peptide obtained by degradation). By using these peptides, it is possible to obtain a fermented product composition having an excellent collagen synthesis effect and cell proliferation effect.

[0020] The method for obtaining the peptide is not particularly limited. The above peptides can usually be obtained by hydrolyzing various proteins with protease, acid, alkali or the like. Of course, the specific hydrolysis method and conditions are not particularly limited. Here, the kind of the protein is not particularly limited. Examples of the protein include soy protein and silk protein contained in silk. Further, as the above peptide, a peptide artificially synthesized by chemically bonding amino acids to each other or by a known genetic engineering technique can be used.

[0021] The number of amino acids and the molecular weight of the peptide are not particularly limited. The lower limit of the number of amino acids of the peptide can be set to 2, 3, or 4, for example. Further, the upper limit of the number of amino acids of the peptide can be, for example, 100, 80, 60, or 40. The lower limit of the molecular weight of the peptide can be set to 50, 100 or 200, for example. In addition, the peptide molecule As the upper limit of quantity, if it is column f, it can be set to 300000, 200000, 100000, 50000 or 30000. In particular, for the purpose of cell proliferation action, it is preferable to use a high molecular weight peptide, for example, a peptide having a molecular weight of 40,000 or more as the peptide. The molecular weight of the peptide can be measured by various known methods. The molecular weight of the peptide can be measured by, for example, electrophoresis using SDS-PAGE and gel filtration chromatography using HPLC and a column. The amino acid number and molecular weight of the peptide can be appropriately adjusted by hydrolyzing the protein or peptide with a protease, acid, alkali, or the like.

[0022] The peptide is preferably (1) a peptide having a molecular weight of 200 to 200,000, (2) a peptide having an amino acid number of 4 to 40 and a molecular weight of 220 to 8000, and (3) an amino acid number force. A mixture of peptides having different molecular weights of ¾ to 3 and molecular weights of 100 or less, 100 to 250, and 250 to 500. Further, as these peptides (1) to (3), silk fiber mouth-in is more preferable. By containing these peptides and the fermented product, it is possible to obtain a fermented product composition having superior collagen synthesizing action, cell proliferating action, and the like as compared with the case of containing only the fermented product.

[0023] Further, as the peptide, a peptide obtained by hydrolyzing soy protein, having 2 to 6 amino acids and having a molecular weight of 100 to 1200 is preferable. By containing a peptide using this soy protein and a fermented product, it is possible to obtain a fermented product composition having superior collagen synthesizing action, cell proliferating action, etc., compared to the case of containing only fermented product. it can.

[0024] The above peptides may be used alone or in combination of two or more. For example, as the peptide, only one of the various peptides described above may be used, or two or more may be used in combination. Moreover, as said peptide, 2 or more types of peptides from which an amino acid number and / or molecular weight differ may be included. Furthermore, as the above-mentioned peptide, two or more kinds of peptides of different kinds, such as a peptide obtained by hydrolyzing soy protein and a silk peptide such as sinoretafive mouth-in, can be used in combination.

[0025] The above-mentioned "Vitamin A" is a compound having an isoprenoid-type polyalcohol as a basic skeleton and a derivative of the compound. Specific examples of vitamin A include, for example, Examples include vitamin Al (retinol), vitamin A2, vitamin A3, and 3,4-dihydroretinol, as well as aldehydes (retinal and the like) and carboxylic acids (retinoic acid) and the like which are oxidized. The “vitamins A” include pharmaceutically acceptable salts and pharmaceutically acceptable derivatives. Examples of the derivatives include carboxylic acid esters such as the above retinol. More specifically, examples of the derivative include C1-C30 esters such as retinoyl acetate and retinol palmitate. In the present invention, the above vitamins may be used alone or in combination of two or more.

[0026] In the present invention, there is no particular limitation on the form of the vitamin A. The vitamin A itself may be contained in combination with other substances that may be contained in the fermented product composition of the present invention. For example, the vitamin A can be contained in the fermented product composition of the present invention as an inclusion compound included in a host compound such as cyclodextrin. The vitamin A can be contained in the fermented product composition of the present invention as a composition or mixture containing the vitamin A. For example, the vitamin A can be contained in the fermented product composition of the present invention as a composition containing the vitamin A such as liver oil. Furthermore, the vitamin A can be contained in the fermented product composition of the present invention as a solution or dispersion in which the vitamin A is dissolved or dispersed. For example, the vitamin A can be dissolved in animal oil or vegetable oil, and the solution can be contained in the fermented product composition of the present invention.

[0027] In the fermented product composition of the present invention, the ratio of the fermented product to the peptide and the vitamin A is not particularly limited. When the total of the fermented product, the peptide and the vitamin A is 100% by mass, the ratio of the fermented product is usually 0.:!-99.9% by mass, preferably 10-80% by mass, More preferably, it is 40-60 mass%. In the present invention, the collagen synthesis action or the like is improved by containing the fermented product, one or more of the above peptides and vitamin A. In the present invention, when the content of each of one or more of the fermented product, the peptide, and the vitamin A is not greatly different, that is, when the mass ratio of the fermented product is 40 to 60% by mass. Is preferable because the collagen synthesis action and the like are further improved.

[0028] The fermented product composition of the present invention can be used for various applications. Especially for this application There is no limitation. The fermented product composition of the present invention can be used, for example, for cosmetics such as external preparations for skin, cosmetic materials, foods and drinks, food and drink additives, bathing agents, and the like.

[0029] The fermented product composition of the present invention can be blended in cosmetics, foods and drinks, and the like. When blended in cosmetics, the blended amount of the fermented product composition of the present invention is not particularly limited. The blending amount can be blended in an appropriate amount depending on the type of cosmetics. The blending amount of the fermented product composition of the present invention can be 0.001 to 100% by mass, especially 0.001 to 50% by mass when the cosmetic is 100% by mass. When the content of the fermented product composition of the present invention is within the above range, a cosmetic product having an excellent collagen synthesis action and the like can be obtained.

[0030] The types of cosmetics to which the fermented product composition of the present invention can be blended are not particularly limited.

The cosmetics include, for example, freeze-drying, packs, pack sheets, peeling, powders, lotions, white towels, solid white lipsticks, lipsticks, solid scarlet, foundation creams, emulsions, lotions, body lotions, cleansing lotions. , Face washing cream, skin care cream, hair rinse, hair liquid, eye shadow and eyebrows. Depending on the type of these cosmetics, other ingredients such as plant sex, vitamins, vitamin-like substances, minerals, lower alcohols, polyhydric alcohols, oils and fats, surfactants are used as necessary. This can be done by adding additives, antioxidants, UV absorbers, thickeners, pigments, preservatives, and fragrances.

[0031] Examples of cosmetics containing the fermented product composition of the present invention include skin external preparations. The fermented product composition of the present invention has an excellent collagen synthesis action and the like. Therefore, the fermented product composition of the present invention is particularly useful for cosmetics used by applying to the skin. The external preparation for skin containing the fermented product composition of the present invention as an active ingredient can be prepared using the fermented product composition of the present invention and other compounding agents used for the external skin preparation. Examples of other binders include liquid oil (such as squalane and jojoba oil), solid oil (such as beeswax and cetyl alcohol), various activators, and humectants (such as glycerin and 1,3-butylene alcohol). Is mentioned. There is no particular limitation on the dosage form of the external preparation for skin. This external preparation for skin can be made into various dosage forms according to the purpose, such as lotion, cream and emulsion. In this external preparation for skin, the blending amount of the fermented product composition of the present invention is not particularly limited. The amount of the fermented product composition of the present invention is 100% by mass when the external preparation for skin is 100% by mass. In other words, the content can be set to 0.001 to 100% by mass, particularly 0.001 to 50% by mass. If the content of the fermented product composition of the present invention is within the above range, it can be used as a skin external preparation having an excellent collagen synthesis action and the like.

[0032] The food and drink containing the fermented product composition of the present invention includes, for example, the fermented product composition of the present invention mixed with other food raw materials or a premixed food raw material mixture of the fermented product composition of the present invention. And then processed by a general method. The food and drink containing the fermented product composition of the present invention is prepared by, for example, dissolving the fermented product composition of the present invention in fats and oils, ethanol, propylene glycol, glycerin or a mixture thereof, and blending it into beverages or solid foods. Can be manufactured. In addition, foods and drinks containing the fermented product composition of the present invention are prepared by mixing the fermented product composition of the present invention with a binder such as gum arabic and dextrin to form a powder or granules, which are used as beverages or solid foods. It can be manufactured by blending. Foods and drinks containing the fermented product composition of the present invention can be provided as health foods and functional foods.

[0033] It should be noted that a blend containing the fermented product composition of the present invention can also be used as a cosmetic material, a bath agent, an additive for food and drink, and the like.

[0034] (2) Cosmetic composition, cell-applied skin external preparation composition, and cosmetic raw material

The cosmetic composition, cell-utilized skin external preparation composition and cosmetic raw material of the present invention are characterized by containing the fermented product composition of the present invention. The contents already described apply to the cosmetic composition, the skin external preparation composition for cell activation, and the fermented composition contained in the cosmetic raw material of the present invention.

[0035] The proportion of the fermented composition in the cosmetic composition, cell-utilized skin external preparation composition, and cosmetic raw material of the present invention is not particularly limited. The proportion of the fermented composition For example, the cosmetic composition of the present invention, a cell activator skin external agent composition and the entire cosmetic raw material is 100 mass ^_0/0, 0.001 to 100 weight ⁰ / _0, can be this a force s and in particular from 0.001 to 50 mass ^_0/0. If the proportion of the fermented product composition is within the above range, it can be made into a cosmetic composition having excellent collagen synthesizing action and the like, a cell-utilized skin external preparation composition, and a cosmetic raw material.

[0036] The type of the cosmetic composition of the present invention is not particularly limited. As the cosmetic composition, For example, freeze-drying, knocking, pack sheet, peeling, white lipstick, solid white, lipstick, solid scarlet, foundation cream, emulsion, lotion, body lotion, cleansing cream, facial cream, skin care cream, hair Rinse, hair liquid, shadow and eyebrows. In addition, the cosmetic composition of the present invention may contain other components (for example, plant extracts, vitamins, vitamin-like substances, mineralols, lower alcohols, polyhydric alcohols, oils and fats, interfaces, depending on the type of the cosmetic composition. Activators, antioxidants, UV absorbers, thickeners, dyes, preservatives and fragrances) can also be added.

[0037] More specifically, the cosmetic composition of the present invention includes a skin external preparation. The fermented product composition has an excellent collagen synthesis action and the like. Therefore, the fermented product composition is particularly useful for cosmetics that are applied to the skin. The said external preparation for skin can be prepared using the said fermented composition and the other compounding agent used for an external preparation for skin. Other compounding agents include, for example, liquid oil (such as squalane and jojoba oil), solid oil (such as micro- and cetyl alcohol), various activators, and humectants (such as glycerin and 1,3-butylene glycol). Is mentioned. There are no particular limitations on the dosage form of this topical skin preparation. This external preparation for skin can be made into various dosage forms according to the purpose, such as lotion, cream and milky lotion. In this external preparation for skin, the amount of the fermented product composition is not particularly limited. The blending amount of the fermented product composition can be 0.001 to 100% by mass, particularly 0.001 to 50% by mass, when the external preparation for skin is 100% by mass. If the content of the fermented product composition is within the above range, it can be used as a skin external preparation having excellent collagen synthesis action and the like.

[0038] The cell-stimulated skin external preparation of the present invention contains the fermented product composition. The fermented product composition has an excellent collagen synthesis action and the like. Therefore, the fermented product composition is particularly useful when blended in cosmetics that are applied to the skin. The description of the above-mentioned skin external preparation can be applied as it is to the cell skin external preparation of the present invention.

[0039] The cosmetic raw material of the present invention includes, for example, freeze-drying, packs, pack sheets, peeling, powder, lotion, white lipstick, solid white lipstick, solid red lipstick, foundation tarm, emulsion, lotion, Used in body lotions, cleansing lotions, facial creams, skin care creams, hair rinses, hair liquids, eye shadows and eyebrows I can.

[0040] (3) Method for producing fermented product composition, etc.

The fermented product composition, cosmetic composition, cell-utilized skin external preparation composition and cosmetic raw material production method of the present invention are fermented products obtained by fermenting rice bran strength and legume-derived peptides with Bacillus subtilis. And a mixing step of mixing at least one of peptide and vitamin A.

[0041] The contents described in the fermented product composition of the present invention are applied to the "rice bran", the "bean-derived peptide", the "Bacillus subtilis", the "peptide" and the "vitamin A". Ru

[0042] The above-mentioned "fermented product" is usually obtained by a fermentation process in which Bacillus subtilis is inoculated and fermented in a medium containing rice bran and legume-derived peptides. The “medium” in the “fermentation step” may be any medium that contains rice bran and a bean-derived peptide and that allows Bacillus subtilis to grow and perform fermentation culture. There is no limitation in particular in the kind and composition of the said culture medium. The medium is usually a liquid medium, but may be a solid medium. Further, the pH of the above medium (especially fermentation B temple) is usually 5 · 5 to 8 · 5, preferably ま or 6 · 0 to 8 · 0, and more preferably ま or 6 · 5 to 7 · 5. In addition, in order to adjust pH and nutritional conditions to a preferable range, saccharides such as gnolecose, enzymes such as protease, water such as purified water, and the like can be added to the medium.

[0043] The culture temperature during the fermentation is not particularly limited as long as the fermentation is performed. The culture temperature is usually 15 to 50 ° C, preferably 20 to 45 ° C, more preferably 30 to 45 ° C, more preferably 35 to 43 ° C. Fermentation culture is usually performed by aeration and agitation.

[0044] The form of the fermented product obtained by the fermentation process is not particularly limited. The contents described in the fermented product composition of the present invention are applied to the fermented product.

[0045] There is no particular limitation on the method of mixing the fermented product with at least one of the peptide and vitamin A in the "mixing step". The fermented product and the peptide can be mixed using an appropriate apparatus such as a mixer such as a homomixer depending on the form and the mass ratio of each. Further, the temperature at the time of mixing is not particularly limited, and is preferably set according to the type of fermented product and peptide. Fermented products and peptides are 5 ~ It can be mixed while cooling if necessary in the range of 20 ° C, and can be mixed while heating if necessary in the range of 35 to 85 ° C.

[0046] The production method of the present invention may further include other steps. As this other process, the process etc. which refine | purify the said fermented material are mentioned, for example. The fermented product obtained by the fermentation process may be mixed with the peptide as it is, or the fermented product may be purified and then mixed with the peptide. The method for this purification treatment is not particularly limited. For example, the fermented product can be purified by pressing and filtering the fermentation broth. In addition, the fermentation broth can be purified by squeezing and filtering the fermented liquor, followed by deodorization with activated charcoal and the like, decoloration treatment, and Z or sediment removal treatment, and then filtering again. .

[0047] The contents of the step of obtaining the cosmetic composition and the cell-applied skin external preparation composition by formulating the fermented product composition are particularly limited as long as the cosmetic composition and the cell-applied skin external preparation composition can be obtained. There is no. Usually, the fermented composition, the cosmetic composition, and other ingredients constituting the cell activation skin external preparation composition are mixed and formulated into a tablet or liquid preparation, so that the cosmetic composition and the cell utilization are obtained. A skin external preparation composition can be obtained. Of course, the above fermented product composition can be directly formulated into a tablet or liquid preparation to make a cosmetic composition and a cell-utilized skin external preparation composition.

Example

Hereinafter, the present invention will be specifically described with reference to examples.

[1] Manufacture of fermented product composition

Rice bran 3 kg, soybean peptide 0.4 kg, glucose 0.5 kg, alkaline protease 0.0 lkg and purified water 50 kg were mixed to prepare a medium. Thereafter, the pH of the medium was adjusted to 6.0 to 8.0, then inoculated with Bacillus natto, and cultured at 37 ° C for 38 hours for fermentation. Thereafter, the fermentation broth was squeezed and filtered, and then decolorized and deodorized with activated carbon. Then, the fermented product was manufactured by filtering and refine | purifying a fermentation liquid again.

[0049] [2] Evaluation of fermented composition containing silk hive mouth-in

The fermented product manufactured in [1] above, vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), and synoleta fibroin (manufactured by Audleman Co., Ltd., trade name “Five Mouth In Sheet”) are each 2% in terms of solid content. (Mass / volume) was dissolved in a buffer solution (PBS). The solution was then added to 0.22 / im It filtered with the filter of. Then, the sample solutions of Experimental Examples 2 to 15 were prepared by diluting and mixing the solutions so that each component had a concentration described in Table 1 and Table 2. In Experimental Example 1, the buffer solution (PBS) was used as a sample solution.

[0050] 5000 human skin fibroblasts per 1 well were distributed in each of two 96-well well plates (referred to as “P1” and “P2” in the table). Subsequently, the cells were cultured for 3 days in a MEM medium supplemented with 5% FBS. Experimental example:! ~ 15 samples were added to MEM medium supplemented with 5% FBS to prepare sample-added medium. Experimental example in the sample-added medium: The concentration of the sample solution of! To 15 is 1% (mass / volume, excluding the mass of the PBS) (therefore, each component in the fermented composition in the medium) The final concentration of is 1 Z 100 of the concentration in the sample solution.) Then, the 5% FBS-added MEM medium in the well was replaced with the sample-added medium, and further cultured for 3 days.

[0051] Next, using a "collagen stin kit" (manufactured by Collagen Technology Research Group), the amount of collagen synthesis (/ g) was measured according to the manual attached to the kit. Tables 1 and 2 show the average values of collagen synthesis in Plate 1 (P1) and Plate 2 (P2). Further, assuming that the collagen synthesis amount of Experimental Example 1 was 1, relative values of the collagen synthesis amounts of Experimental Examples 2 to 8 were obtained. Similarly, assuming that the collagen synthesis amount of Experimental Example 9 is 1, the relative value of the collagen synthesis amount of Experimental Examples 10 to 15 was determined. The results are also shown in Tables 1 and 2. In Tables 1 and 2, “VC” means vitamin C, and “SF” means silk fiber mouth-in.

[0052] [Table 1]

Kola's single synthesis amount

(5 formulas Relative value Average value)

P 1 P2 P 1 P2

1 PBS (-) 100% 1. 00 1. 00 1. 21 1. 30

2 VC 1% 1. 07 1. 18 1. 9 1. 52 Actual 3 Fermented product 1% 1. 22 1. 05 1. 47 1. 36

4 SF 1% 1. 27 1. 18 1. 53 1. 52

5 Fermented product 0.5% + SF 0.5% 1. 49 1. 44 1. 80 1. 87 Example 6 Fermented product 1. 0% + SF 1. 0% 1. 75 1. 77 2. 1 1 2 . 29

7 Fermented product 1. 8% + SF 0. 2% 1. 40 1. 26 1. 68 1. 64

8 Fermented product 0.2% + SF 1. 8% 1. 75 1. 54 2. 1 1 1. 99 [Table 2]

2

From Tables 1 and 2, in Experimental Examples 5 to 8 and 13 to 15 that use both silk-five mouth-in and fermented products, only Experimental Examples 3 and 11 that use only fermented products and Silk-five mouth-in are used. It can be seen that the collagen synthesizing action is superior to those of Experimental Examples 4 and 12. [0055] [3] Evaluation of fermented product compositions containing peptides derived from other silk raw materials

Fermented product manufactured in [1] above, vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), Shinoleta five mouth-in, and the following silk peptides (a) and (b) are each 2% (mass / volume) in solid content concentration So that it was dissolved in a buffer solution (PBS). The solution was then filtered through a 0.22 zm fineletter. Thereafter, the sample solutions of Experimental Examples 17-: 19, 24-28, and 33-35 were prepared by diluting and mixing the solutions so that each component had a concentration described in Tables 3 to 5. . Moreover, the sample solutions of Experimental Examples 20-22, 29-31, and 36 were prepared by mixing the sample solutions of Experimental Examples 17-: 19, 24-28, and 33-35 in equal amounts by volume. In Experimental Examples 16, 23 and 32, the buffer solution (PBS) was used as a sample solution.

[0056] Vitamin C and silk fiber mouth-in are the same as those used in [2] above. In addition, the following were used as silk peptides (a) and (b). Silk peptides (a) and (b) were both liquids, and were diluted with a buffer solution so that the final concentration as a solid content concentration was 1% (mass Z volume).

Silk peptide (a); Cosmo Foods, trade name "Silk Peptide M-500" Synolec peptide (b); Cosmo Foods, trade name "Sinolec peptide M-500 # 60"

[0057] Collagen synthesis amount g) of Experimental Examples 16 to 36 was measured by the same method as in [2] above.

Tables 3 to 5 show the average values of collagen synthesis in Plate 1 (P1) and Plate 2 (P2). Further, assuming that each collagen synthesis amount in Experimental Examples 16, 23 and 32 was 1, the relative value of the collagen synthesis amount in other experimental examples was determined. The results are also shown in Tables 3-5. In Tables 3 to 5, “VC” means vitamin C, “SF” means silk five mouth in, and “SPJ means silk peptide”.

[0058] [Table 3] Table 3

[0059] [Table 4]

Table 4

[0060] [Table 5] Table 5

[0061] According to the results of Tables 3 to 5, in Experimental Examples 22, 29, and 36 using the sinoletafive mouth-in and the fermented product, Experimental Examples 18, 25, and 34 using only the fermented product, In addition, as compared with Experimental Examples 19, 26 and 35 using only silk fiber mouth-in, it has a superior collagen synthesizing action as described above. Furthermore, it can be seen that Experimental Examples 30 and 31 in which the silk peptide and the fermented material are used in combination also have a superior collagen synthesizing action as in the case of sinoleta five mouth-in. From Experimental Examples 17, 24 and 33, it can be seen that vitamin C also has a collagen synthesizing action. However, in Experimental Example 20 in which fermented product and vitamin C are used in combination and in Experimental Example 21 in which vitamin C and silk fiber mouth-in are used in combination, the synergistic effect of the combined use is not observed, or is found to be very slight. Therefore, it can be seen that the synergistic effect of the combined use of the fermented product and the peptide is large.

[0062] [4] Evaluation of fermented product and composition containing soy peptide

Fermented product manufactured in [1] above, Vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), Synoletafibu mouth-in, and the following soybean peptides (a) to (c), each with a solid content concentration of 2% (mass / volume) The sample was dissolved in a buffer solution (PBS). The solution was then filtered through a 0.22 / im filter. Thereafter, the solutions were diluted and mixed so that each component had a concentration described in Table 6 and Table 7, thereby preparing sample solutions of Experimental Examples 38 to 43 and 49 to 54. Moreover, the sample solutions of Experimental Examples 44 to 47 and 55 to 56 were prepared by mixing equal amounts of the sample solutions of Experimental Examples 38 to 43 and 49 to 54 by volume. In Experimental Examples 37 and 48, the sample solution was The buffer solution (PBS) was used.

[0063] Vitamin C and silk fiber mouth-in are the same as those used in [2] above. The following were used as soybean peptides (a) to (c). The soybean peptides (a), (b), and (c) were all liquids, and were used after being diluted with a buffer solution so that the final concentration as a solid content concentration was 1% (mass Z volume).

Soy peptide (a): Fuji Oil Co., Ltd.

Soy peptide (b): Fuji Oil Co., Ltd.

Soy peptide (c); Fuji Oil Co., Ltd., trade name “Hynyute DC5”

[0064] By the same method as in [2] above, the amount of collagen synthesis (μg) in Experimental Examples 37 to 47 was measured.

Table 6 shows the average value of the amount of collagen synthesis in Plate 1 (P1) and Plate 2 (P2). Further, assuming that the amount of collagen synthesis in Experimental Example 37 was 1, the relative value of the amount of collagen synthesis in other Experimental Examples was determined. The results are also shown in Table 6. In Table 6, “VC” means Vitamin C, “SF” means Synoletafibu Mouth Inn, and 8? (&) To ((:) ”are soybean peptides (&) to (c). means.

[0065] [Table 6]

Table 6

[0066] Each of the two 96-well plate was distributed with 2000 human skin fibroblasts per well. Subsequently, the cells were cultured for 3 days in a MEM medium supplemented with 5% FBS. Thereafter, the medium was replaced with a serum-free MEM medium and further cultured for 1 day. 0.5% serum-added MEM medium A sample-added medium was prepared by adding the samples of Experimental Examples 48-56. The concentration of the sample solutions of the above Experimental Examples 48 to 56 in the sample-added medium is 1% (mass Z volume, excluding the mass of the PBS). The final concentration of the component is 1/100 of the concentration in the sample solution.) Then, the serum-free MEM medium in the well was replaced with the sample-added medium, and further cultured for 4 days.

[0067] Then, based on Dojindo “Cell Counting Kit 8”, WST-8 tetrazolium salt was used as a chromogenic substrate, and water-soluble formazan produced by reduction by dehydrogenase in living cells was measured at a wavelength of 450 nm. Then, the absorbance was measured (the number of living cells is proportional to the absorbance value). Further, assuming that the number of living cells in Experimental Example 48 was 1, the relative value of the amount of living cells in other Experimental Examples was calculated, and the cell proliferation action was evaluated. The results are also shown in Table 7. In Table 7, “¥ 0” means vitamin 0, “SF” means silk-five mouth-in, and “SBP (a) to (c)” means soybean peptides (a) to (c). The average value of the cell growth rate term in Table 7 is the average value of the absorbance values.

[0068] [Table 7] Table 7

[0069] From Table 6, Experimental Example 44 using both silk-five mouth-in and fermented product compared to Experimental Example 39 using only fermented product and Experimental Example 40 using only Synoleta-Five mouth-in, It can be seen that it has a better collagen synthesis action. Furthermore, it can be seen that Experimental Examples 45 to 47 in which the fermented product and the soybean peptide are used in combination also have a superior collagen synthesizing action as in the case of Synoletafibu mouth-in.

[0070] From Table 7, Experimental Example 56 using both the fermented product and soybean peptide (c) has a more improved cell proliferation effect than Experimental Example 50 using only the fermented product. I understand that. Furthermore, it can be seen that Experimental Example 55 using both the fermented product and the sinoletafib mouth-in also improves the cell proliferation action.

[0071] [5] Evaluation of fermented product composition containing vitamin A

Fermented product manufactured in [1] above, vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), and cyclodextri Inclusion retinol (trade name “CAVAMAX W8 / Retinol Complex” manufactured by Cyclochem) was dissolved in a buffer solution (PBS) so that the solid concentration was 2% (mass / volume). The solution was then filtered through a 0.22 / m filter. Thereafter, the solutions were diluted so that each component had a concentration shown in Table 8 and mixed to prepare sample solutions of Experimental Examples 58 to 66. In Experimental Example 57, the buffer solution (PBS) was used as the sample solution.

[0072] The collagen synthesis amount (z g) and cell proliferation rate of Experimental Examples 57 to 66 were measured by the same method as described in [2] and [4] above. The average value is shown in Table 8. Further, assuming that the amount of collagen synthesis and the amount of living cells in Experimental Example 57 was 1, the relative values of the amount of collagen synthesis and the amount of living cells in other Experimental Examples were determined. The results are also shown in Table 8. In Table 8, “◯” means vitamin C, and “CDR” means cyclodextrin inclusion retinol. Moreover, the average value of the term of cell growth rate in Table 8 is the average value of absorbance values.

[0073] [Table 8] Table 8

[0074] From Table 8, Experimental Examples 64 to 66 using both retinol and fermented product are compared to Experimental Example 59 using only fermented product and Experimental Examples 60 to 63 using only retinol, Excellent It can be seen that it has a collagen synthesizing action and a cell proliferating action.

[0075] [6] Evaluation of fermented product composition containing peptide and vitamin A

The fermented product produced in [1] above, the silk fiber mouth-in, the soybean peptide (a), and the cyclodextrin inclusion retinol in solid content concentrations of 2, respectively. It was dissolved in a buffer solution (PBS) so as to be / ₀ (mass / volume). The solution was then filtered through a 0.22 zm fineletter. Thereafter, the sample solutions of Experimental Examples 68, 69, 71, and 72 were prepared by diluting and mixing the solutions so that each component had a concentration described in Table 9 and Table 10. In Experimental Examples 67 and 70, the buffer solution (PBS) was used as the sample solution.

[0076] By the same method as in [2] and [4] above, the amount of collagen synthesis (x g) and cell proliferation rate of Experimental Examples 67 to 72 were measured. The average values are shown in Table 9 and Table 10. Further, with the amount of collagen synthesis and the amount of living cells in Experimental Examples 67 and 70 as 1, the relative values of the amount of collagen synthesis and the amount of living cells in other Experimental Examples were determined. The results are also shown in Table 9 and Table 10. In Tables 9 and 10, “SF” means silk-five mouth-in, “SBP” means soybean peptide, and “CDR” means cyclodextrin inclusion retinol. In Table 9 and Table 10, the average value of the term of cell proliferation rate is the average value of absorbance.

[0077] [Table 9]

Table 9

Yes

[0079] From Table 10, Experimental Examples 69 and 72 using retinol and a peptide together with a fermented product are as follows: It can be seen that it has excellent collagen synthesis action and cell proliferation action.

[0080] The present invention is not limited to the specific examples described above. The present invention can be variously modified within the scope of the present invention depending on the purpose and application.

Industrial applicability

[0081] The fermented product composition of the present invention is excellent in safety and has a collagen synthesis action and the like. The fermented product composition of the present invention can be used by blending it with various cosmetics such as external preparations for skin and various foods such as health foods and functional foods. That is, the present invention can be used in the fields of cosmetics and foods and drinks.

Claims

The scope of the claims

[1] A fermented product composition comprising a fermented product obtained by fermenting rice bran strength and a bean-derived peptide with Bacillus subtilis, and at least one of a peptide and vitamin A. .

[2] The fermented product composition according to claim 1, wherein the peptide is at least one of a peptide obtained by hydrolyzing soy protein and silk fibroin.

[3] A cosmetic composition comprising the fermented product composition according to claim 1.

[4] A fermented product composition comprising a mixing step of mixing a fermented product obtained by fermenting rice bran and bean-derived peptides with Bacillus subtilis and at least one of peptides and vitamin A. Production method.

[5] a mixing step of mixing a fermented product obtained by fermenting rice bran and bean-derived peptides with Bacillus subtilis and at least one of peptides and vitamins A;

And a step of formulating the fermented product composition obtained in the mixing step to obtain a cosmetic composition.