CN102225045B - Fermented product composition, cosmetic composition and method of producing the same - Google Patents
Fermented product composition, cosmetic composition and method of producing the same Download PDFInfo
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- CN102225045B CN102225045B CN2011101610504A CN201110161050A CN102225045B CN 102225045 B CN102225045 B CN 102225045B CN 2011101610504 A CN2011101610504 A CN 2011101610504A CN 201110161050 A CN201110161050 A CN 201110161050A CN 102225045 B CN102225045 B CN 102225045B
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- peptide
- fermented product
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- composition
- product composition
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Classifications
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- A—HUMAN NECESSITIES
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Abstract
A fermented product composition and a cosmetic composition comprising a fermented product, which is obtained by fermenting peptides originating in rice bran and beans by using Bacillus subtilis, and at least one member selected from peptides and vitamin A analogs. A production method comprising a mixing step wherein a fermented product, which is obtained by fermenting peptides originating in rice bran and beans by using Bacillus subtilis, is mixed with at least one member selected from peptides and vitamin A analogs.
Description
The application is to be that May 2, application number in 2007 are dividing an application of 200780015790.9 application of the same name the applying date.
Technical field
The present invention relates to fermented product composition, cosmetic composition and their manufacture method.More particularly, the present invention relates to a kind of safe, and the excellent fermented product composition such as ossein anabolic effect.And, the invention still further relates to the cosmetic composition that contains this fermented product composition.And then, the invention still further relates to the manufacture method of above-mentioned fermented product composition and cosmetic composition.
Background technology
Former known cosmetics such as skin preparations for extenal use that contain the fermented product that makes the fermentation such as Testa oryzae and obtain etc.For example, the cosmetic composition that contains fermentation bran class and the cosmetics that adopt this compositions are disclosed in the patent documentation 1.Put down in writing in the patent documentation 1, this cosmetics moisture retention is good, and the stimulation when being coated on the skin is few, is difficult to be inflamed, and is very comfortable.And, disclose in the patent documentation 2 by bacillus subtilis and made the fermentation of Testa oryzae and soybean peptide, the manufacture method of the skin preparations for extenal use raw material that then obtains by purification process.It is high to have put down in writing this skin preparations for extenal use materials safety in the patent documentation 2, can improve the activity of skin.
Patent documentation 1: JP 2004-262829 communique
Patent documentation 2: Unexamined Patent 8-104647 communique
Summary of the invention
The problem that invention will solve
The function that produces by the fermented product that cooperates patent documentation 1 and 2 records in the cosmetics such as skin preparations for extenal use is many.For example, cooperated the cosmetics such as skin preparations for extenal use of this fermented product can suppress wrinkle, speckle, lax etc., cold injury, chapped and skin roughening etc.And, in the cosmetics such as skin preparations for extenal use that cooperated this fermented product, obtained the texts that improves of the symptoms such as trauma care and burn.
The object of the present invention is to provide safe, and the excellent fermented product composition such as ossein anabolic effect.And, the object of the invention is to by containing the cosmetic composition of this fermented product composition.And then, the object of the present invention is to provide the manufacture method of above-mentioned fermented product composition and cosmetic composition.
For the method for dealing with problems
The present invention is following content.
(1) a kind of fermented product composition is characterized in that containing:
The fermented product that the peptide fermentation that Testa oryzae and beans is originated by bacillus subtilis obtains, and
At least one in peptide and the retinoid.
(2) fermented product composition of above-mentioned (1) record, above-mentioned peptide are that soybean protein is through the peptide of hydrolysis acquisition and at least one in the fibroin albumen (Si1k fibroin).
(3) a kind of cosmetic composition is characterized in that it contains the fermented product composition of above-mentioned (1) record.
(4) a kind of manufacture method of fermented product composition, it is characterized in that the method have will make by bacillus subtilis the blend step that mixes mutually of the fermented product that obtains of the peptide fermentation in Testa oryzae and beans source and at least one in peptide and the retinoid.
(5) a kind of manufacture method of cosmetic composition, it is characterized in that the method have will make by bacillus subtilis the blend step that mixes mutually of the fermented product that obtains of the peptide fermentation in Testa oryzae and beans source and at least one in peptide and the retinoid, and the step by the fermented product composition preparation acquisition cosmetic composition that will obtain in this blend step.
The effect of invention
Fermented product composition of the present invention with natural goods as raw material.Therefore, fermented product composition of the present invention is safe, and has excellent ossein anabolic effect and cel l proliferation etc.
And above-mentioned peptide is soybean protein when being hydrolyzed the peptide that forms and at least one in the fibroin albumen (Silkfibroin), easier acquisition, and can form the fermented product composition with synthetic property of sufficient ossein etc.
Therefore cosmetic composition of the present invention plays excellent ossein anabolic effect and cel l proliferation owing to have above-mentioned formation.
If according to the manufacture method of fermented product composition of the present invention and cosmetic composition, just can by using the bacillus subtilis that can obtain easily, make it to ferment to make.Therefore, can have the above-mentioned excellent fermented product composition of the present invention that acts on etc. by small-sized manufacturing equipment safety and easily manufacturing.
For the best mode that carries out an invention
Below, describe the present invention in detail.
(1) fermented product composition
Fermented product composition of the present invention is characterised in that and contains the fermented product that the peptide fermentation in Testa oryzae and beans source is obtained by bacillus subtilis, and at least one in peptide and these two kinds of materials of retinoid.
As above-mentioned " Testa oryzae ", can enumerate, for example, Testa oryzae, defatted rice bran, Rice plumule and defat Rice plumule.Can use separately a kind in these, also can use simultaneously more than 2 kinds.Above-mentioned Testa oryzae is not limited to commercially available Testa oryzae etc., can use the Testa oryzae of various species.
Above-mentioned " beans source peptide " if the peptide of contained protein source in contained peptide or the beans in the beans, there is no particular limitation for its concrete kind and structure.Above-mentioned " peptide in beans source " is not limited to the peptide in commercially available beans source, can use the peptide in various beans source.As the peptide in above-mentioned beans source, for example can enumerate soybean peptide, little bean peptide, Semen Pisi sativi peptide and Semen Viciae fabae peptide.As the peptide in above-mentioned beans source, preferred soybean peptide.The peptide in above-mentioned beans source can use separately a kind, also can use simultaneously more than 2 kinds.
There is no particular limitation to obtain the method for peptide in above-mentioned beans source.For example, the peptide in above-mentioned beans source can obtain by extracting beans (for example, Semen sojae atricolor, Semen Phaseoli, pea and bean etc. are one kind or two or more) with solvent (water, hot water, alcohol (ethanol etc.) organic solvent or water-organic solvent mixed solvent).In this process, can carry out classification and purification etc. as required.And then, by the processing that is hydrolyzed with protease, acid or alkali, can be to peptide or the further fragmentation of bean protein in the beans source that obtains.And then the peptide in above-mentioned beans source also can be by making between the aminoacid with the combination of chemical mode peptide, or the peptide by known gene step method synthetic.
There is no particular limitation for the form of the peptide in above-mentioned beans source.The peptide in above-mentioned beans source can be after said extracted, by formation solidified solid matters (for example, powder or shot-like particle etc.) such as dryings.And the peptide as above-mentioned beans source can use said extracted thing self.That is to say, among the present invention, can use the extract (beans extract, especially soybean extract etc.) of the peptide that contains above-mentioned beans source as the peptide in above-mentioned beans source.For example, among the present invention, peptide as above-mentioned beans source, can use the extract that obtains by extract beans (for example, in Semen sojae atricolor, Semen Phaseoli, the pea and bean etc. one kind or two or more) with solvent (organic solvent or the water-organic solvent mixed solvents such as water, hot water, alcohol (ethanol etc.)).Among the present invention, can preferably use extract (soybean extract) that especially contains soybean peptide etc.
Be used for to obtain to contain the extracting method of extract of the peptide in above-mentioned beans source, the special restriction not with regard to extraction conditions.For example, as the beans of extracting raw material, can not pulverize, can pulverize yet.And, as long as can keep the quality of extract, also can remove the pre-treatment of impurity etc.And, as extracting solvent, except water or hot water, can also use the mixed solvent of the organic solvent such as the esters such as alcohol, ethyl acetate, the normal hexane such as methanol and ethanol and these organic solvents and water or hot water etc.As the said extracted solvent, the mixed solvent of preferred water or hot water, alcohol (methanol and ethanol etc.) and water or hot water and alcohol.The temperature of above-mentioned hot water is generally 40～100 ℃, and preferred 50～80 ℃, more preferably 50～70 ℃.And the pH that extracts solvent during extraction is generally 3～7, and is preferred 4～6, more preferably 4～5.Why preferred, be because by pH being adjusted in the above-mentioned scope, can keep extracting the stability of various compositions contained in the raw material.The extraction temperature has no particular limits, but preferred room temperature or heating extraction.During heating extraction, as heating-up temperature, usually at 40～100 ℃, preferred 50～80 ℃, more preferably 50～70 ℃.Why preferably because by heating-up temperature being adjusted to this scope, can effectively extract.
There is no particular limitation for the ratio of the peptide in above-mentioned Testa oryzae and above-mentioned beans source.Usually, when the peptide in above-mentioned beans source was 100 mass parts, the ratio of above-mentioned Testa oryzae was 1～20 mass parts, preferred 5～20 mass parts, more preferably 8～18 mass parts.If above-mentioned Testa oryzae in above-mentioned scope, then can obtain to have the fermented product of sufficient ossein anabolic effect etc.
Above-mentioned " bacillus subtilis " (Bacillus subtilis) be the representation type of aerobic spore forming bacteria.The kind of above-mentioned bacillus subtilis is as long as safety is guaranteed, and then there is no particular limitation.As above-mentioned bacillus subtilis, for example can use Bacillus natto (Bacillus natto) etc.As above-mentioned bacillus subtilis, the preferred easy cheap Bacillus natto that obtains again.And, as above-mentioned bacillus subtilis, can use commercially available general bacillus subtilis.And, as long as can obtain to have the fermented product of ossein anabolic effect etc., as above-mentioned bacillus subtilis, can use mycology property nature or that generate with aritifical variant's means such as the chemical substances such as nitrosoguanidine, x-ray and ultraviolet that the variant of variation has occured.
Above-mentioned " fermented product " can obtain the peptide fermentation in above-mentioned Testa oryzae and above-mentioned beans source by bacillus subtilis.Above-mentioned fermented product usually can be by absorbing bacillus subtilis in the culture medium of the peptide that contains above-mentioned Testa oryzae and above-mentioned beans source, fermentation culture obtains under proper condition.There is no particular limitation for the fermentation culture conditions that obtains above-mentioned " fermented product ".Fermentation culture is undertaken by air agitation usually.And with regard to above-mentioned culture medium, as long as above-mentioned bacillus subtilis can breed, just there is no particular limitation.Above-mentioned culture medium is fluid medium normally, but also can be solid medium.And, the pH of above-mentioned culture medium (when especially fermenting) usually 5.5～8.5, preferred 6.0～8.0, more preferably 6.5～7.5.Why preferred, be because if this pH is positioned at above-mentioned scope, just can keep extracting the stability of various compositions contained in the raw material.And then with regard to cultivation temperature, as long as can ferment, then there is no particular limitation.This cultivation temperature is usually at 15～50 ℃, and preferred 20～45 ℃, more preferably 30～45 ℃, more preferably 35～43 ℃.And, can also in culture medium, add the various nutrients such as sugar and be used for adjust the bronsted lowry acids and bases bronsted lowry etc. of pH.And then above-mentioned fermentation can be mixed culture fermentation, also can be continuous fermentation.
There is no particular limitation for the form of above-mentioned fermented product.Above-mentioned fermented product can be to filter the stock solution that obtains by fermentation liquid or this fermentation liquid process that fermentation culture obtains.And above-mentioned fermented product can be to above-mentioned fermentation liquid, carries out as required sterilization treatment or pH and adjusts, or utilize ion exchange resin, activated-charcoal column or dialyzer etc. to carry out the fermentation liquid of the post processings such as deodorization, decolouring.And then above-mentioned fermented product can be that this fermentation liquid has been carried out concentrated concentrated solution or paste.In addition, above-mentioned fermented product can also be by known methods such as lyophilizations this fermentation liquid have been removed the solids of solvent and the powder thing of powdered.And then above-mentioned fermented product can also be as the solution or the dispersion liquid that have added above-mentioned fermentation liquid or above-mentioned solid matter and powder thing in the mixed solvent of the organic solvent such as water or ethanol, propylene glycol and 1,3 butylene glycol or these solvents.
Above-mentioned " peptide " be aminoacid by peptide in conjunction with and the polypeptide of combination.And above-mentioned aminoacid is generally L-aminoacid, but also can be D-aminoacid, or the mixture of the two.That is to say that the aminoacid that consists of above-mentioned peptide can be any in L-aminoacid, D-aminoacid and the mixture of the two.
The kind of above-mentioned peptide, there is no particular limitation for source and structure.For example, above-mentioned peptide can be the peptide of plant origin contained in the plant, also can be the peptide of animal origin contained in the animal.And the structure of above-mentioned peptide not only for the straight chain structure, can also contain two sulfur combinations.As above-mentioned peptide, particularly, for example can enumerate the silk peptide such as the peptide that obtains by hydrolysing soybean protein (in the Semen sojae atricolor contained protein) and fibroin albumen (peptide that obtains by contained protein in peptide contained in the hydrolyzed-silk and the silk) etc.If use these peptides, just can obtain to have the fermented product composition of excellent ossein anabolic effect and cel l proliferation etc.
There is no particular limitation to obtain the method for above-mentioned peptide.Above-mentioned peptide usually can be by obtaining with hydrolysis range proteins such as protease, acid or alkali.Certainly, concrete method for hydrolysis and condition be special the restriction not.Wherein, there is no particular limitation for the kind of above-mentioned protein.As above-mentioned protein, can enumerate, for example contained silk protein in soybean protein and the silk.And, as above-mentioned peptide, can also use by chemically making between the aminoacid peptide combination occurs, or the peptide by known gene engineering method synthetic.
There is no particular limitation for the amino acid no of above-mentioned peptide and molecular weight.The lower limit of the amino acid no of above-mentioned peptide can be for example 2,3 or 4.And the upper limit of the amino acid no of above-mentioned peptide can be for for example, 100,80,60 or 40.The lower limit of the molecular weight of above-mentioned peptide can be for for example, and 50,100 or 200.And, as the upper limit of the molecular weight of above-mentioned peptide, can be for for example, 300000,200000,100000,50000 or 30000.Especially, with cel l proliferation during as purpose, as above-mentioned peptide, preferably use the peptide of high molecular, for example, molecular weight is the peptide more than 40000.And the molecular weight of above-mentioned peptide can be measured by known the whole bag of tricks.The molecular weight of above-mentioned peptide can be measured by gel permeation chromatography of carrying out such as the electrophoresis method that is undertaken by SDS-PAGE with by HPLC and post etc.The amino acid no of above-mentioned peptide and molecular weight can be by suitably regulating with the aminosal such as protease, acid or alkali or peptide.
As above-mentioned peptide, be preferably (1) molecular weight peptide, (2) amino acid no of 200～200000 be 4～40 and molecular weight be 220～8000 peptide and (3) amino acid no be 2～3 and molecular weight be below 100, the mixture of 100～250 peptides different with 250～500 molecular weight.And, as the peptide of these (1)～(3), more preferably fibroin albumen.By making it to contain these peptides and above-mentioned fermented product, compare when only containing fermented product, can obtain to have the fermented product composition of more excellent ossein anabolic effect and cel l proliferation etc.
And, as above-mentioned peptide, can be that soybean protein obtains through hydrolysis, the preferred amino acid number is 2～6, and molecular weight is 100～1200 peptide.Adopt this soy proteinaceous peptide and fermented product by making it to contain, compared when only containing fermented product, can obtain to have the fermented product composition of more excellent ossein anabolic effect and cel l proliferation etc.
Above-mentioned peptide can use separately a kind, also can use more than 2 kinds.For example, as above-mentioned peptide, can only use a kind in the above-mentioned various peptide, also can use more than 2 kinds.And, as above-mentioned peptide, can contain the different peptide more than 2 kinds of amino acid no and/or molecular weight.And then, as above-mentioned peptide, can also use simultaneously the diverse peptides more than 2 kinds such as silk peptide such as the peptide that obtains through hydrolysis as soybean protein and fibroin albumen.
Above-mentioned " retinoid " is to have isoprenoid type polyenoid alcohol as the derivant of chemical compound He this chemical compound of basic framework.As the said vitamin category-A, particularly, for example can enumerate the aldehyde (retinal etc.) that retinol1 (retinol), dehydroretinol, 3-Hydroxyretinol and 3,4-dihydro retinol and these materials are oxidized and carboxylic acid (tretinoin) etc.And, in above-mentioned " retinoid ", also comprise pharmaceutically admissible salt and pharmaceutically admissible derivant.As said derivative, for example can enumerate the carboxylate of above-mentioned retinol etc.As said derivative, more specifically, can enumerate, for example, the C1 such as retinyl acetate and retinyl palmitate～C30 ester etc.And among the present invention, the said vitamin class both can be used separately a kind, also can use simultaneously more than 2 kinds.
Among the present invention, there is no particular limitation for the form that contains of said vitamin category-A.Can make and contain said vitamin category-A self in the fermented product composition of the present invention, it also can share and be contained in the above-mentioned composition with other material.For example, the said vitamin category-A as making it the clathrate of enclose in host compounds such as cyclodextrin, can make it to be contained in the fermented product composition of the present invention.And the said vitamin category-A as the compositions that contains the said vitamin category-A or mixture, can make it to be contained in the fermented product composition of the present invention.For example, the said vitamin category-A as the compositions that contains the said vitamin category-As such as liver oil, can make it to be contained in the fermented product composition of the present invention.And then, the said vitamin category-A, solution or dispersion liquid as dissolving or disperseed the said vitamin category-A can make it to be contained in the fermented product composition of the present invention.For example, the said vitamin category-A is dissolved in animal oil or the vegetable oil, this solution is contained in the fermented product composition of the present invention.
In the fermented product composition of the present invention, there is no particular limitation for above-mentioned fermented product and above-mentioned peptide and said vitamin category-A ratio.When above-mentioned fermented product and above-mentioned peptide and said vitamin category-A added up to 100 quality %, the ratio of above-mentioned fermented product was generally 0.1～99.9 quality %, preferred 10～80 quality %, more preferably 40～60 quality %.Among the present invention, owing to contain above-mentioned fermented product and one kind or two or more above-mentioned peptide and said vitamin category-A, so the raising such as ossein anabolic effect.Among the present invention, because when above-mentioned fermented product and one kind or two or more each content that is selected from the material in above-mentioned peptide and the said vitamin category-A do not have significant difference, be the mass ratio of above-mentioned fermented product when being 40～60 quality %, ossein anabolic effect etc. further improve, thereby preferred.
Fermented product composition of the present invention can be used for various uses.There is no particular limitation for this purposes.Fermented product composition of the present invention can for example, use in the cosmetics such as skin preparations for extenal use, cosmetic material, diet product, diet product usefulness additive and the bath foam etc.
Fermented product composition of the present invention can cooperate in cosmetics and diet product etc.When cooperating in cosmetics, there is no particular limitation for the use level of fermented product composition of the present invention.This use level can be according to an amount of cooperations the such as kind of cosmetics.The use level of fermented product composition of the present invention when cosmetics are 100 quality %, can be 0.001～100 quality %, especially 0.001～50 quality %.If the amount of fermented product composition of the present invention in above-mentioned scope, then can be made the cosmetics with excellent ossein anabolic effect etc.
There is no particular limitation can to cooperate the kind of cosmetics of fermented product composition of the present invention.As these cosmetics, for example can enumerate lyophilization thing, Pack (pack), sheet-type packs (pack sheet), peeling (peeling), powder, astringent, cosmetic powder, solid cosmetic powder, lipstick, solid rouge, foundation cream, emulsion, astringent, watch box breast, cleansing milk, cleansing cream, protective skin cream, hair rinse, shampoo, eye shadow and eyebrow pencil etc.And, in these cosmetics, according to its kind, as required, can also add other composition, for example, plant extract, vitamin, vitamin substances, mineral, lower alcohols, multivalence alcohols, oils, surfactant, antioxidant, UV absorbent, viscosifier, pigment, antiseptic and spice etc.
As the cosmetics that cooperate fermented product composition of the present invention, especially can enumerate skin preparations for extenal use.Fermented product composition of the present invention has excellent ossein anabolic effect etc.Therefore, fermented product composition of the present invention is particularly useful in coating the cosmetics that skin uses.Containing fermented product composition of the present invention can prepare with fermented product composition of the present invention and other compounding ingredient that can use in skin preparations for extenal use as the skin preparations for extenal use of effective ingredient.As above-mentioned other compounding ingredient, can enumerate such as liquid oil (Squalene and leaves of pulse plants leaves of pulse plants fragrant plant oil (Jojoba oil) etc.), solid oil (Cera Flava and spermol etc.), various activating agent and wetting agent (glycerol and 1,3 butylene glycol etc.).There is no particular limitation for the dosage form of this skin preparations for extenal use.This skin preparations for extenal use for example can form, and astringent, cream and emulsion etc. are suitable for the various dosage forms of purpose.In this skin preparations for extenal use, there is no particular limitation for the use level of fermented product composition of the present invention.The use level of fermented product composition of the present invention when skin preparations for extenal use is 100 quality %, can be 0.001～100 quality %, especially 0.001～50 quality %.If the content of fermented product composition of the present invention in above-mentioned scope, then can form the skin preparations for extenal use with excellent ossein anabolic effect etc.
The diet product that contain fermented product composition of the present invention, can for example pass through, fermented product composition of the present invention and other raw-food material are mixed together, and perhaps cooperate fermented product composition of the present invention in the raw-food material mixture that is pre-mixed, and then process to make with general method.The diet product that contain fermented product composition of the present invention can be dissolved in oils and fats, ethanol, propylene glycol, glycerol or their mixture fermented product composition of the present invention by for example, it is matched with in beverage or the solid food makes.And, contain the diet product of fermented product composition of the present invention, thereby can make powder or granule etc. by fermented product composition of the present invention is mixed mutually with bonding agent such as Radix Acaciae senegalis and dextrin, it is matched with in beverage or the solid food makes again.The diet product that contain fermented product composition of the present invention can be used as health food and functional food etc. to be provided.
And, cooperated the coordination compound of fermented product composition of the present invention to use with additive etc. as cosmetic material, bath foam and diet product.
(2) cosmetic composition, cell activation Dermatologic preparation composition and cosmetic material
Cosmetic composition of the present invention, cell activation are characterised in that with Dermatologic preparation composition and cosmetic material and contain fermented product composition of the present invention.Cosmetic composition of the present invention, cell activation are suitable for the content that has illustrated with above-mentioned fermented product composition contained in Dermatologic preparation composition and the cosmetic material.
Cosmetic composition of the present invention, there is no particular limitation for the ratio of above-mentioned fermented product composition in cell activation usefulness Dermatologic preparation composition and the cosmetic material.The ratio of above-mentioned fermented product composition for example, when cosmetic composition of the present invention, cell activation totally are 100 quality % with Dermatologic preparation composition and cosmetic material, can be 0.001～100 quality %, especially 0.001～50 quality %.If the ratio of above-mentioned fermented product composition in above-mentioned scope, then can form the cosmetic composition with excellent ossein anabolic effect etc., cell activation is with Dermatologic preparation composition and cosmetic material.
There is no particular limitation for the kind of cosmetic composition of the present invention.As above-mentioned cosmetic composition, for example can enumerate lyophilization thing, Pack (pack), sheet-type packs (pack sheet), peeling (peeling), cosmetic powder, solid cosmetic powder, lipstick, solid rouge, foundation cream, emulsion, astringent, watch box breast, cleansing milk, cleansing cream, protective skin cream, hair rinse, shampoo, eye shadow and eyebrow pencil.And, in the cosmetic composition of the present invention, according to its kind, can also add as required other composition (for example, plant extract, vitamin, vitamin substances, mineral, lower alcohols, multivalence alcohols, oils, surfactant, antioxidant, UV absorbent, viscosifier, pigment, antiseptic and spice).
As cosmetic composition of the present invention, can enumerate more specifically, especially skin preparations for extenal use.Above-mentioned fermented product composition has excellent ossein anabolic effect etc.Therefore, fermented product composition of the present invention is particularly useful in coating the cosmetics that skin uses.Above-mentioned skin preparations for extenal use can prepare with above-mentioned fermented product composition and other compounding ingredient that can use in skin preparations for extenal use.As above-mentioned other compounding ingredient, for example can enumerate, liquid oil (Squalene and leaves of pulse plants leaves of pulse plants fragrant plant oil (Jojoba oil) etc.), solid oil (Cera Flava and spermol etc.), various activating agent and wetting agent (glycerol and 1,3 butylene glycol etc.).There is no particular limitation for the dosage form of this skin preparations for extenal use.This skin preparations for extenal use can form the various dosage forms that astringent, cream and emulsion etc. are suitable for purpose.In this skin preparations for extenal use, there is no particular limitation for the use level of above-mentioned fermented product composition.The use level of above-mentioned fermented product composition when skin preparations for extenal use is 100 quality %, can be 0.001～100 quality %, especially 0.001～50 quality %.If the content of fermented product composition in above-mentioned scope, then can form the skin preparations for extenal use with excellent ossein anabolic effect etc.
Cell activation of the present invention contains above-mentioned fermented product composition with skin preparations for extenal use.Above-mentioned fermented product composition has excellent ossein anabolic effect etc.It is particularly useful when therefore, above-mentioned fermented product composition cooperates in coating the cosmetics that skin uses.Cell activation of the present invention is with the explanation of itself applicable above-mentioned skin preparations for extenal use of skin preparations for extenal use.
Cosmetic material of the present invention can be for example, and lyophilization thing, Pack (pack), sheet-type packs (pack sheet), peeling (peeling), powder, astringent, cosmetic powder, solid cosmetic are with being used in powder, lipstick, solid rouge, foundation cream, emulsion, astringent, watch box breast, cleansing milk, cleansing cream, protective skin cream, hair rinse, shampoo, eye shadow and the eyebrow pencil.
(3) manufacture method of fermented product composition etc.
Fermented product composition of the present invention, cosmetic composition, cell activation are characterised in that to have the blend step that fermented product that the peptide fermentation that will make by bacillus subtilis Testa oryzae and beans source obtains and at least one in peptide and these two kinds of materials of retinoid mix mutually with the manufacture method of Dermatologic preparation composition and cosmetic material.
Above-mentioned " Testa oryzae ", above-mentioned " peptide in beans source ", above-mentioned " bacillus subtilis ", above-mentioned " peptide " and above-mentioned " retinoid ", the content that illustrates in the applicable fermented product composition of the present invention.
Above-mentioned " fermented product ", the fermentation step that usually can make it to ferment by the inoculation of medium bacillus subtilis at the peptide that contains Testa oryzae and beans source obtains.Above-mentioned " culture medium " in above-mentioned " fermentation step " contains the peptide in Testa oryzae and bean source, can be the culture medium that can carry out fermentation culture by bacillus subtilis propagation.There is no particular limitation for the Species and composition of above-mentioned culture medium.Above-mentioned culture medium is fluid medium normally, also can be solid medium.And the pH of above-mentioned culture medium (when especially fermenting) is generally 5.5～8.5, preferred 6.0～8.0, more preferably 6.5～7.5.And, in the above-mentioned culture medium, for pH and nutritional condition etc. is adjusted to preferable range, can add water such as the enzymes such as saccharide, protease, purified water such as glucose etc.
Cultivation temperature during above-mentioned fermentation then has no particular limits so long as can ferment.This cultivation temperature is generally 15～50 ℃, is preferably 20～45 ℃, and more preferably 30～45 ℃, more preferably 35～43 ℃.Fermentation culture is undertaken by air agitation usually.
The form of the above-mentioned fermented product that obtains by above-mentioned fermentation step has no particular limits.The content that illustrates in the applicable fermented product composition of the present invention of the form of above-mentioned fermented product.
In above-mentioned " blend step ", there is no particular limitation with method that at least one in above-mentioned peptide and these two kinds of materials of retinoid mixes mutually for above-mentioned fermented product.Fermented product and peptide can according to separately form and mass ratio separately etc., mix with suitable devices such as the blenders such as mixer for well-distribution (Homomixer).And there is no particular limitation for the temperature during mixing, preferably according to settings such as fermented product and peptide various species.Fermented product and peptide mix while can cool off in 5～20 ℃ scope as required, mix while can also heat in 35～85 ℃ scope as required.
Manufacture method of the present invention can also possess other step again.As this other step, for example can enumerate, above-mentioned fermented product is carried out the step of purification process etc.The fermented product that obtains by fermentation step can mix with original peptide, perhaps also can carry out purification process to this fermented product, and then mix with peptide.There is no particular limitation for the method for this purification process.For example, by the squeezing fermentation liquid, refilter, can the above-mentioned fermented product of purification.And, also can refilter by fermentation liquid is squeezed, then by active carbon etc. carry out deodorization, decolouring is processed, and/or the processing of disgorging, then, by again filtering the above-mentioned fermented product of purification.
As long as can obtain cosmetic composition and cell activation Dermatologic preparation composition by thereby above-mentioned fermented product composition preparation being obtained cosmetic composition and cell activation with the content of the step of Dermatologic preparation composition, in that there is no particular limitation.Usually, by above-mentioned fermented product composition is mixed with cell activation other raw material with Dermatologic preparation composition mutually with the formation cosmetic composition, make the preparations such as tablet or liquor, can obtain cosmetic composition and cell activation Dermatologic preparation composition.Certainly, can also be with above-mentioned fermented product composition in statu quo, or by making the preparations such as tablet or liquor, make cosmetic composition and cell activation Dermatologic preparation composition.
Embodiment
Below, specify the present invention by embodiment.
[1] manufacturing of fermented product composition
Mix 3kg Testa oryzae, 0.4kg soybean peptide, 0.5kg glucose, 0.01kg alkaline protease and 50kg purified water, the preparation culture medium.Then, the pH of culture medium is adjusted to 6.0～8.0, then, the inoculation Bacillus natto is cultivated in 37 ℃ and to be fermented in 38 hours.Then, squeezing and filtering fermentating liquid, then, with activated carbon decolorizing, deodorization.Then carry out purification by filtering fermentating liquid again, thereby prepare fermented product.
[2] contain the evaluation of the fermented product composition of fibroin albumen (Silk fibroin)
Fermented product with preparation in above-mentioned [1], vitamin C (with the pure pharmaceutical worker's industry of light society system) and fibroin albumen (Silk fibroin) (Baudeleman company system, trade name " Fibroin Sheet ") are dissolved in the buffer (PBS), so that the solid component concentration of each material reaches 2% (mass/volume).Then, the filter with 0.22 μ m filters this solution.Then, by dilution and mix this solution so that each composition reaches in table 1 and the table 2 concentration of record, thus the sample solution of preparation experiment example 2～15.And experimental example 1 uses above-mentioned buffer (PBS) as sample solution.
(be called " P1 " and " P2 " in the table at 2 96 orifice plates.) on, 5000 individual's skin fibroblasts are all inoculated in per 1 hole.Then, in having added the MEM culture medium of 5%FBS, cultivated 3 days.Prepare sample by the sample that in having added the MEM culture medium of 5%FBS, adds experimental example 1～15 and add culture medium.The concentration that this sample adds the sample solution of experimental example 1～15 in the culture medium is 1% (mass/volume, but the quality of removing above-mentioned PBS.) (therefore, in the culture medium in the fermented product composition ultimate density of each composition be in the sample solution concentration 1/100.)。And, the above-mentioned MEM culture medium of having added 5%FBS in the hole is replaced with said sample adds culture medium, cultivated again 3 days.
Then, use " ossein staining kit " (ossein technical research meeting system), measure ossein synthetic quantity (μ g) according to the handbook that test kit is subsidiary.The meansigma methods of ossein synthetic quantity is shown in table 1 and table 2 in plate 1 (P1) and the plate 2 (P2).And the ossein synthetic quantity of experimental example 1 is obtained the relative value of the ossein synthetic quantity of experimental example 2～8 as 1.Equally, the ossein synthetic quantity of experimental example 9 is obtained the relative value of the ossein synthetic quantity of experimental example 10～15 as 1.Its result adds up in table 1 and table 2.And in table 1 and table 2, " VC " refers to vitamin C, and " SF " refers to fibroin albumen (Silk fibroin).
Table 1
Table 2
According to table 1 and table 2, known in the experimental example 5～8 and 13～15 that uses at the same time fibroin albumen and fermented product that compare with 12 with the experimental example 4 of fibroin albumen with 11 with the experimental example 3 that only uses fermented product, its ossein anabolic effect is excellent.
[3] contain the evaluation of fermented product composition of the peptide of other raw material sources
With the fermented product of making in above-mentioned [1], vitamin C (with the pure pharmaceutical worker's industry of light society system), fibroin albumen and following silk peptide (a) and (b) be dissolved in the buffer (PBS) are so that the solid component concentration of each material reaches respectively 2% (mass/volume).Then, by dilution and mix this solution so that each composition reaches in table 3～table 5 concentration of record, thus preparation experiment example 17～19,24～28 and 33～35 sample solution.And, by the sample solution of equivalent combined experiments example 17～19,24～28 on volume and 33～35, prepare experimental example 20～22,29～31 and 36 sample solution.And, in experimental example 16,23 and 32, use above-mentioned buffer (PBS) as sample solution.
Vitamin C and middle use identical in fibroin albumen and above-mentioned [2].And, as silk peptide (a) and (b), use following material.And, silk peptide (a) and (b) be any one liquid, the final concentration that is diluted to as solid component concentration with buffer is 1% (mass/volume), re-uses.
Silk peptide (a); Cosmo food society system, trade name " silk peptide M-500 "
Silk peptide (b); Cosmo food society system, trade name " silk peptide M-500#60 "
By the method identical with above-mentioned [2], the ossein synthetic quantity of determination experiment example 16～36 (μ g).The meansigma methods of the ossein synthetic quantity in plate 1 (P1) and the plate 2 (P2) is shown in table 3～table 5.And experimental example 16, each ossein synthetic quantity of 23 and 32 are obtained the relative value of the ossein synthetic quantity of other experimental example as 1.Its result adds up in table 3～table 5.And in table 3～table 5, " VC " refers to vitamin C, and " SF " refers to fibroin albumen, and " SP " represents silk peptide.
Table 3
Table 4
Table 5
Result according to table 3～5, use at the same time in the experimental example 22,29 and 36 of fibroin albumen and fermented product, with the experimental example 18 that only uses fermented product, 25 and 34, and only use the experimental example 19,26 of fibroin albumen to compare with 35, have equally a more excellent ossein anabolic effect with aforementioned.And, also known, use at the same time the experimental example 30 and 31 of silk peptide and fermented product, the same with the situation of fibroin albumen, have more excellent ossein anabolic effect.And, according to experimental example 17,24 and 33, known that vitamin C has the ossein anabolic effect.But, use at the same time fermented product and ascorbic experimental example 20 and use simultaneously in the experimental example 21 of vitamin C and fibroin albumen, do not have to find that owing to using simultaneously the synergy that produces perhaps this effect is minimum.Therefore, known that the synergy that uses simultaneously fermented product and peptide to produce is large.
[4] contain the evaluation of the fermented product composition of soybean peptide
Fermented product with preparation in above-mentioned [1], vitamin C (with the pure pharmaceutical worker's industry of light society system), fibroin albumen and following soybean peptide (a)～(c) are dissolved in the buffer (PBS), so that the solid component concentration of each material all reaches 2% (mass/volume).Then, the filter with 0.22 μ m filters this solution.Then, by dilution and mix this solution so that each composition reaches in table 6 and the table 7 concentration of record, thus the sample solution of preparation experiment example 38～43 and 49～54.And, by the sample solution of mixed in equal amounts experimental example 38～43 by volume and 49～54, preparation experiment example 44～47 and 55～56 sample solution.And, in experimental example 37 and 48, use above-mentioned buffer (PBS) as sample solution.
Vitamin C and middle use identical in fibroin albumen and above-mentioned [2].And, as soybean peptide (a)～(c), use following material.And, soybean peptide (a), (b) and (c) be any one liquid, the final concentration that is diluted to as solid component concentration with buffer is 1% (mass/volume), re-uses.
Soybean peptide (a); Only liquefaction society system, trade name " Ha ィ ニ ュ one ト SMP "
Soybean peptide (b); Only liquefaction society system, trade name " Ha ィ ニ ュ one ト R "
Soybean peptide (c); Only liquefaction society system, trade name " Ha ィ ニ ュ one ト DC5 "
By the method identical with above-mentioned [2], the ossein synthetic quantity of determination experiment example 37～47 (μ g).The meansigma methods of the ossein synthetic quantity in plate 1 (P1) and the plate 2 (P2) is shown in table 6.And the ossein synthetic quantity of experimental example 37 is obtained the relative value of the ossein synthetic quantity of other experimental example as 1.Its result adds up in table 6.And in table 6, " VC " refers to vitamin C, and " SF " refers to fibroin albumen, and " SBP (a)～(c) " refers to soybean peptide (a)～(c).
Table 6
On 2 96 orifice plates, 2000 individual's skin fibroblasts are all scattered in per 1 hole.Then, in having added the MEM culture medium of 5%FBS, cultivated 3 days.Then, be replaced with serum-free MEM culture medium, cultivated again 1 day.Prepare sample by the sample that in having added the MEM culture medium of 5%FBS, adds experimental example 48～56 and add culture medium.The concentration that this sample adds the sample solution of above-mentioned experimental example 48～56 in the culture medium is 1% (mass/volume, but the quality of removing above-mentioned PBS.) (therefore, in the culture medium in the fermented product composition ultimate density of each composition be in sample solution concentration 1/100.)。And, the above-mentioned serum-free MEM culture medium in the hole is replaced with said sample adds culture medium, cultivated again 4 days.
Then, based on Tongrentang " cell counting test kit 8 ", with the WST-8 tetrazolium salts as chromophoric substrate, measure the water-soluble Xing formazan (Formazan) that generates by the reduction of the dehydrogenase in the living cells under the wavelength of 450nm, (viable count is proportional with the value when this absorbance to measure absorbance.)。And, as 1, calculate the relative value of the amount of viable cell of other experimental example with the viable count of experimental example 48, estimate cel l proliferation.The result adds up in table 7.And in the table 7, " VC " refers to vitamin C, and " SF " refers to fibroin albumen (Silk fibroin), and " SBP (a)～(c) " refers to soybean peptide (a)～(c).And this meansigma methods of cell proliferation rate is the meansigma methods of above-mentioned absorbance in the table 7.
Table 7
According to table 6, known, use at the same time the experimental example 44 of fibroin albumen and fermented product, compare with the experimental example 40 of the experimental example 39 that only uses fermented product and fibroin albumen, have more excellent ossein anabolic effect.And, use at the same time the experimental example 45～47 of fermented product and soybean peptide, the same with the situation of fibroin albumen, have more excellent ossein anabolic effect.
According to table 7, known the experimental example 56 that uses simultaneously fermented product and soybean peptide (c), to compare with the experimental example 50 that only uses fermented product, its cel l proliferation further improves.And, using at the same time the experimental example 55 of fermented product and fibroin albumen, cel l proliferation improves too.
[5] contain the evaluation of the fermented product composition of retinoid
Fermented product with preparation in above-mentioned [1], vitamin C (with the pure pharmaceutical worker's industry of light society system) and CAVAMAXW8/RetinolComplex (trade name " CAVAMAXW8/RetinolComplex " シ Network ロ ケ system society system) are dissolved in the buffer (PBS), so that the solid component concentration of each material reaches respectively 2% (mass/volume).Then, the filter with 0.22 μ m filters this solution.Then, so that each composition reaches the concentration of record in the table 8, prepare the sample solution of experimental example 58～66 by diluting this solution.And, in the experimental example 57, use above-mentioned buffer (PBS) as sample solution.
With the method identical with the method for above-mentioned [2] and [4] record, the ossein synthetic quantity of determination experiment example 57～66 (μ g) and cell proliferation rate.Its meansigma methods is shown in table 8.And, as 1, obtain the ossein synthetic quantity of other experimental example and the relative value of amount of viable cell with the ossein synthetic quantity of experimental example 57 and amount of viable cell.This result adds up in table 8.And in table 8, " VC " refers to vitamin C, and " CDR " refers to CAVAMAXW8/RetinolComplex.And this meansigma methods of cell proliferation rate is the meansigma methods of absorbance in the table 8.
[table 8]
According to table 8, known the experimental example 64～66 that uses simultaneously retinol and fermented product, compare with the experimental example 60～63 that only uses retinol with the experimental example 59 that only uses fermented product, have more excellent ossein anabolic effect and cel l proliferation.
[6] contain the evaluation of the fermented product composition of peptide and retinoid
With prepared fermented product in above-mentioned [1], above-mentioned fibroin albumen, above-mentioned soybean peptide (A) and above-mentioned CAVAMAXW8/RetinolComplex are dissolved in the buffer (PBS), so that the solid component concentration of each material all reaches 2% (mass/volume).Then, the filter with 0.22 μ m filters this solution.Then, by dilution and mix this solution so that each composition reaches in table 9 and the table 10 concentration of record, thus preparation experiment example 68,69,71 and 72 sample solution.And, in experimental example 67 and 70, use above-mentioned buffer (PBS) as sample solution.
By the method identical with [4] with above-mentioned [2], the ossein synthetic quantity of determination experiment example 67～72 (μ g) and cell proliferation rate.Its meansigma methods is shown in table 9 and table 10.And, as 1, obtain the ossein synthetic quantity of other experimental example and the relative value of amount of viable cell with the ossein synthetic quantity of experimental example 67 and 70 and amount of viable cell.Its result adds up in table 9 and table 10.And in table 9 and the table 10, " SF " refers to that fibroin albumen, " SBP " refer to soybean peptide, and " CDR " refers to CAVAMAXW8/RetinolComplex.And this meansigma methods of cell proliferation rate is the meansigma methods of absorbance in table 9 and table 10.
According to table 10, known that the experimental example 69 and 72 of the fermented product that uses simultaneously retinol and peptide has excellent ossein anabolic effect and cel l proliferation.
And, the invention is not restricted to above-mentioned specific embodiment.The present invention is according to purpose, and purposes can be carried out various variations within the scope of the invention.
The probability of industrial utilization
Fermented product composition safety of the present invention is good, has ossein anabolic effect etc.Fermented product composition of the present invention can be used in the various cosmetics such as skin preparations for extenal use and the various diet product such as health food and functional food etc.That is to say that the present invention can use in the fields such as cosmetics and diet product.
The present invention includes following content:
1, a kind of fermented product composition is characterized in that containing:
The fermented product that the peptide fermentation that Testa oryzae and beans is originated by bacillus subtilis obtains, and
At least one in peptide and the retinoid.
2, the fermented product composition described in the project 1, above-mentioned peptide are that soybean protein is through the peptide of hydrolysis acquisition and at least one in the fibroin albumen.
3, a kind of cosmetic composition is characterized in that it contains project 1 described fermented product composition.
4, a kind of manufacture method of fermented product composition, it is characterized in that the method have will make by bacillus subtilis the blend step that mixes mutually of the fermented product that obtains of the peptide fermentation in Testa oryzae and beans source and at least one in peptide and the retinoid.
5, a kind of manufacture method of cosmetic composition, it is characterized in that the method have will make by bacillus subtilis the blend step that mixes mutually of the fermented product that obtains of the peptide fermentation in Testa oryzae and beans source and at least one in peptide and the retinoid, and the step by the fermented product composition preparation acquisition cosmetic composition that will obtain in this blend step.
Claims (4)
1. fermented product composition is characterized in that containing:
The fermented product that the peptide fermentation that Testa oryzae and beans is originated by bacillus subtilis obtains, and
Peptide;
The peptide in wherein said beans source is soybean peptide,
The described peptide peptide that to be soybean protein obtain through hydrolysis and at least one in the fibroin albumen,
In the described fermentation, the pH of culture medium is 5.5～8.5, and cultivation temperature is 15-50 ℃,
Described fermented product mixes with 9: 1～1: 9 ratio of mass ratio with described peptide.
2. a cosmetic composition is characterized in that it contains fermented product composition claimed in claim 1.
3. the manufacture method of a fermented product composition is characterized in that the method has the blend step that fermented product that the peptide fermentation that will make by bacillus subtilis Testa oryzae and beans source obtains and peptide mix mutually,
The peptide in wherein said beans source is soybean peptide,
The described peptide peptide that to be soybean protein obtain through hydrolysis and at least one in the fibroin albumen,
In the described fermentation, the pH of culture medium is 5.5～8.5, and cultivation temperature is 15-50 ℃,
Described fermented product mixes with 9: 1～1: 9 ratio of mass ratio with described peptide.
4. the manufacture method of a cosmetic composition, it is characterized in that the method have will make by bacillus subtilis the blend step that mixes mutually of the fermented product that obtains of the peptide fermentation in Testa oryzae and beans source and peptide, and the step by the fermented product composition preparation acquisition cosmetic composition that will obtain in this blend step
The peptide in wherein said beans source is soybean peptide,
The described peptide peptide that to be soybean protein obtain through hydrolysis and at least one in the fibroin albumen,
In the described fermentation, the pH of culture medium is 5.5～8.5, and cultivation temperature is 15-50 ℃,
Described fermented product mixes with 9: 1～1: 9 ratio of mass ratio with described peptide.
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| KR20130058107A (en) * | 2011-11-25 | 2013-06-04 | (주)아모레퍼시픽 | Isolation and preparation of small peptides from soybean and its cosmetic composition |
| CN102846537B (en) * | 2012-10-18 | 2014-01-29 | 天津市可美国际贸易有限公司 | Preparation method of skin care essence stoste |
| JP6332941B2 (en) * | 2013-11-01 | 2018-05-30 | 株式会社東洋発酵 | Oral composition for beauty health |
| CN106466291B (en) * | 2015-08-19 | 2019-06-07 | 德州昂立达生物技术有限公司 | A kind of still cleaning products and preparation method thereof containing fermented grain object |
| CN105596286A (en) * | 2016-01-25 | 2016-05-25 | 广州保税区雅兰国际化妆品有限公司 | Yeast skin-whitening cosmetic composition and preparation method thereof |
| CN110484569A (en) * | 2019-09-18 | 2019-11-22 | 上海应用技术大学 | A kind of meter of source fermentation material and the preparation method and application thereof |
| CN112022792B (en) * | 2020-09-27 | 2022-12-09 | 黑龙江省中医药科学院 | Preparation method of edible traditional Chinese medicine hair dye with homology of medicine and food |
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| JP3417419B2 (en) * | 1992-07-13 | 2003-06-16 | 株式会社資生堂 | External preparation for skin |
| JP3625501B2 (en) * | 1994-10-06 | 2005-03-02 | 御木本製薬株式会社 | Method for producing raw material for skin-applied skin and cell-applied skin external agent |
| JPH10234301A (en) * | 1997-02-27 | 1998-09-08 | Meiji Seika Kaisha Ltd | Green tea beverage |
| DE19713092A1 (en) * | 1997-03-27 | 1998-10-01 | Wacker Chemie Gmbh | Complexes of gamma-cyclodextrin and retinol or retinol derivatives, as well as processes for their preparation and their use |
| JP4755325B2 (en) * | 1997-11-04 | 2011-08-24 | 一丸ファルコス株式会社 | Bioactive composition derived from silk protein hydrolyzate |
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| JPWO2002080862A1 (en) * | 2001-04-06 | 2004-07-29 | 株式会社東洋発酵 | Beauty cosmetic material and its manufacturing method |
| WO2004009731A1 (en) * | 2002-07-23 | 2004-01-29 | Fuji Oil Company, Limited | Antioxidant and process for producing the same |
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