TWI393780B - A fermentation composition, a cosmetic composition, and the like - Google Patents

Description

Ferment composition, cosmetic composition, and manufacturing method therefor

The present invention relates to a fermentate composition, a cosmetic composition, and a method of producing the same. More specifically, the present invention relates to a fermented product composition which is extremely excellent in safety, collagen synthesis, and the like. Further, the present invention relates to a cosmetic composition containing the composition of the fermented product. Further, the present invention is more related to the method for producing the above-described fermented product composition and cosmetic composition.

Conventionally, there are cosmetics such as skin external preparations containing a fermented product such as fermented rice bran. For example, Patent Document 1 discloses a cosmetic composition containing fermented rice bran and a cosmetic using the same. Patent Document 1 discloses that the cosmetic is excellent in moisturizing property, and is less irritating when applied to the skin, and is difficult to be inflamed. Further, Patent Document 2 discloses a method for producing a skin external preparation raw material obtained by fermenting rice bran and soybean peptide by subtilis bacteria and then performing refining treatment. Patent Document 2 discloses that the skin external preparation raw material has high safety and can improve skin activity.

Patent Document 1: Japanese Laid-Open Patent Publication No. 2004-262829 (Patent Document 2)

The effects of the fermented product described in Patent Documents 1 and 2 on cosmetics such as external preparations for skin are extremely high. For example, a cosmetic such as a skin external preparation containing the fermented product can suppress wrinkles, spots, loose skin, and can prevent frostbite, erythema, and rough skin. Moreover, the cosmetics such as the external preparation for skin of the fermented product can also be used to improve the effects of wound healing and scald embolism.

The present invention is intended to provide a ferment composition which is excellent in safety, collagen synthesis, and the like. Further, the present invention has an object of providing a cosmetic composition containing the composition of the fermented product. Further, the present invention has an object of providing a method for producing the above-described fermented product composition and cosmetic composition.

The present invention is as follows.

[1] A fermented product characterized by comprising a fermented product obtained by fermenting a peptide derived from rice bran and beans by a Bacillus subtilis, and at least one of a peptide and a vitamin A.

[2] The fermented product composition according to the above [1], wherein the peptide is a peptide in which at least one of soybean protein is hydrolyzed and silk fibroin.

[3] A cosmetic composition comprising the fermented product composition according to [1] above.

[4] A method for producing a composition for a fermented product, comprising the step of mixing a fermentation product obtained by fermenting a peptide derived from rice bran and beans with a Bacillus subtilis, and mixing with at least one peptide and vitamin A.

[5] A method for producing a cosmetic composition, comprising the step of mixing a fermented product obtained by fermenting a peptide derived from rice bran and beans with Bacillus subtilis, a step of mixing with at least one peptide and vitamin A, and obtaining the obtained in the mixing step The fermentant composition preparation is carried out in the step of obtaining a cosmetic composition.

The ferment composition of the present invention is based on natural materials. Therefore, the fermented product composition of the present invention has high safety, and has excellent collagen synthesis and cell proliferation effects.

Further, the peptide is more easily obtained when at least one peptide in which soybean protein is hydrolyzed and silk fibroin, and may be a composition of a fermented product having sufficient collagen synthesizing properties and the like.

The cosmetic composition of the present invention can exhibit an excellent collagen synthesis action and cell proliferation action by having the above-described constitution.

According to the method for producing a fermented product composition and a cosmetic composition of the present invention, it can be produced by fermentation of Bacillus subtilis. Therefore, the above fermented product composition of the present invention having excellent effects can be produced safely and easily by a small manufacturing apparatus.

The invention is described in detail below.

(1) Fermentation composition

The fermented product composition of the present invention is characterized by containing a fermented product obtained by fermenting rice bran with a peptide derived from legumes, and at least one peptide and vitamin A.

The above rice bran may be, for example, rice bran, defatted rice bran, rice germ, and defatted rice germ. These may be used alone or in combination of two or more. The rice bran is not limited to the rice bran of the market, and various types of rice bran can be used.

The "peptide derived from beans" is a peptide derived from a peptide of a legume or a protein contained in a legume, and the type and structure thereof are not particularly limited. The above-mentioned "peptide derived from beans" is not necessarily limited to a peptide derived from a soybean, and a peptide derived from various beans can be used. The above peptides derived from beans may be soybean peptide, red bean peptide, pea peptide and broad bean peptide. The above peptide derived from beans is preferably a peptide derived from soybean. The peptides derived from the above-mentioned beans may be used alone or in combination of two or more.

The method for obtaining the above peptide derived from beans is not particularly limited. For example, the above peptide derived from beans may be extracted with a solvent (water, hot water, an organic solvent such as alcohol (ethanol, etc.) or a water-organic solvent mixed solvent (for example, one or more kinds of soybeans, red beans, peas, and broad beans). In this step, it may be subjected to fractional distillation, purification, etc., and may be hydrolyzed with a protease, an acid or a base to further fragment the obtained peptide or legume protein. The above-mentioned peptide derived from legume may also be a peptide which chemically binds each amino acid to a peptide or artificially synthesized by a known genetic engineering method.

The form of the above-mentioned peptide derived from beans is not particularly limited. The peptide derived from the bean may be a solid (for example, a powder or a granule) which is solidified by drying or the like after the above extraction. Further, as the above-mentioned peptide derived from beans, the above extract itself can be used. That is, in the present invention, as the peptide derived from the legumes, an extract (legume extract, particularly soybean extract, etc.) containing the above-mentioned peptide derived from legumes may be used. For example, in the present invention, as the above-mentioned peptide derived from beans, beans (for example, soybean, red bean, pea, and broad bean) may be extracted by using a solvent (an organic solvent of water, hot water, alcohol (ethanol, etc.) or a water-organic solvent mixed solvent). One or more of the obtained extracts. In the present invention, it is preferred to use an extract (soybean extract) or the like containing a soybean peptide.

The extraction method of the above extract containing the peptide derived from legumes is not particularly limited. For example, the beans used as the raw materials for extraction may be unpulverized or crushed. Further, as long as the quality of the extract can be maintained, it is also possible to apply a pretreatment such as removing impurities. Further, as the extraction solvent, in addition to water or hot water, an alcohol such as methanol or ethanol, an ester such as ethyl acetate, an organic solvent such as n-hexane, or a mixed solvent of such an organic solvent and water or hot water may be used. As the above extraction solvent, water or hot water, alcohols (methanol, ethanol, etc.) and water or a mixed solvent of hot water and alcohol are preferred. The temperature of the hot water is usually 40 to 100 ° C, preferably 50 to 80 ° C, more preferably 50 to 70 ° C. Further, the pH of the extraction solvent at the time of extraction is usually 3 to 7, preferably 4 to 6, more preferably 4 to 5. When the pH is within the above range, it is preferable since the stability of various components contained in the extraction raw material can be maintained. Although the extraction temperature is not particularly limited, it is preferably extracted at room temperature or by heating. In the case of heating extraction, the heating temperature is usually 40 to 100 ° C, preferably 50 to 80 ° C, more preferably 50 to 70 ° C. It is preferred that the heating temperature is within this range and extraction can be carried out efficiently.

The ratio of the above rice bran to the above-mentioned peptide derived from beans is not particularly limited. Usually, when the peptide derived from the bean is 100 parts by mass, the ratio of the rice bran is 1 to 20 parts by mass, preferably 5 to 20 parts by weight, more preferably 8 to 18 parts by mass. As long as the rice bran is in the above range, a fermented product having a sufficient collagen synthesis action or the like can be obtained.

The above Bacillus subtilis (Bacillus subtilis) is a representative species of aerobic sporulation bacteria. The type of the above-mentioned subtilis bacteria is not particularly limited as long as it is a safety guarantee. The above-mentioned subtilis bacteria may be, for example, Bacillus natto or the like. The above-mentioned subtilis bacteria are easily obtained, and the cheap natto bacteria is suitable. In addition, as the above-mentioned Bacillus subtilis, the general Bacillus subtilis which is marketed is available. Further, as long as a fermented product having a collagen synthesis action or the like can be obtained, it can be produced naturally or by a chemical substance such as nitrosoguanidine, X-ray or ultraviolet light, or the like, and the bacteriological property is deteriorated. The mutant strain.

The above "fermented product" is obtained by fermenting the rice bran and the above-mentioned peptide derived from beans by Bacillus subtilis. The above fermented product is usually obtained by ingesting a Bacillus subtilis in a medium containing the rice bran and the above-mentioned peptide derived from legumes, and fermenting it under appropriate conditions. The fermentation culture conditions for obtaining the above "fermentation product" are not particularly limited. The fermentation culture system is usually carried out under aeration and agitation. Further, the above medium is not particularly limited as long as it can proliferate Bacillus subtilis. The above medium is usually a liquid medium, but may also be a solid medium. Further, the pH of the medium (especially during fermentation) is usually 5.5 to 8.5, preferably 6.0 to 8.0, more preferably 6.5 to 7.5. When the pH is within the above range, the stability of various components contained in the extraction raw material can be maintained, so that it is suitable. Further, the culture temperature is not particularly limited as long as it can be fermented. The culture temperature is usually 15 to 50 ° C, preferably 20 to 45 ° C, more preferably 30 to 45, and the optimum is 35 to 43 ° C. Further, various nutrients such as sugar and an acid or a base for adjusting the pH may be added to the medium. In addition, the above fermentation may be a mixed fermentation or a continuous fermentation.

The form of the above fermentation is not particularly limited. The fermented product may be a fermentation broth obtained by fermentation culture or a liquid obtained by filtering the fermentation broth. Further, the fermented product may be a fermentation liquid obtained by subjecting the fermentation liquid to a sterilization treatment or a pH adjustment, or a post-treatment such as deionization or decolorization using an ion exchange resin, an activated carbon layer column or a dialysis membrane. Further, the fermented product may be a concentrate or paste in which the fermentation liquid is concentrated. Further, the fermented product may be a solid matter and a powdered powder obtained by removing a solvent of the fermentation liquid by a known method such as freeze drying. Further, the fermented product may be a solution or dispersion in which the fermentation broth or the solid matter and the powder are added to water, an organic solvent such as ethanol, propylene glycol or 1,3-butanediol or a mixed solvent thereof.

The above "peptide" is an amino acid which is a polypeptide which is bonded by a peptide bond. Further, the above amine group is usually an L-amino acid, but may be a D-amino acid or a mixture of the two. That is, the amino acid constituting the above peptide may be any of L-amino acid, D-amino acid, and a mixture of the two.

The type, origin and structure of the above peptide are not particularly limited. For example, the peptide may be a peptide derived from a plant contained in a plant, or may be a peptide derived from an animal contained in an animal. Further, the structure of the above peptide may be not only a linear structure but also a disulfide-containing bond. Specifically, the peptide may be, for example, a peptide obtained by hydrolysis of soybean protein (protein contained in soybean), and a silk fibroin such as silk fibroin (a peptide which is contained in a silk fibroin and hydrolyzed by a protein contained in silk fibroin). )Wait. When these peptides are used, a ferment composition having a collagen synthesis action, a cell proliferation action, and the like can be obtained.

The method for obtaining the above peptide is not particularly limited. Usually, the above peptide can be obtained by hydrolyzing various proteins by a protease, an acid, a base or the like. Of course, the specific hydrolysis method and conditions are not particularly limited. Here, the type of the above protein is not particularly limited. The above protein may be, for example, soybean protein and silk fibroin contained in silk cocoons. Further, the above peptide may be a peptide which is artificially synthesized by chemically bonding the amino acids to each other or by a genetic engineering method known in the art.

The number and molecular weight of the amino acid of the above peptide are not particularly limited. The lower limit of the number of amino acids of the above peptide is, for example, 2, 3 or 4. Further, the upper limit of the number of amino acids of the above peptide may be, for example, 100, 80, 60 or 40. The lower molecular weight of the above peptide may be, for example, 50, 100 or 200. Further, the upper limit of the molecular weight of the above peptide may be, for example, 300,000, 200,000, 100,000, 50,000 or 30,000. Particularly, for the purpose of cell proliferation, the peptide is preferably a peptide having a molecular weight of 40,000 or more, using a high molecular weight peptide. The molecular weight of the above peptide can be measured by various known methods. The molecular weight of the above peptide can be determined by, for example, SDS-PAGE electrophoresis and HPLC and column gel filtration chromatography. The number and molecular weight of the amino acid of the above peptide can be appropriately adjusted by hydrolyzing a protein or peptide such as a protease, an acid or a base.

The peptide system preferably has (1) a peptide having a molecular weight of 200 to 200,000 (2) a peptide having an amino acid number of 4 to 40, a molecular weight of 220 to 8,000, and (3) a number of amino acids of 2 to 3, And a molecular weight of 100 or less, a mixture of peptides having different molecular weights of 100 to 250 and 250 to 500. Further, it is preferred that the peptides of (1) to (3) are made of silk fibroin. By containing these peptides and the above-mentioned fermented product, it is possible to obtain a fermented product composition having a superior collagen synthesis action, cell proliferation action, and the like than those containing only the fermented product.

Further, the peptide may be a peptide in which soybean protein is hydrolyzed, an amino acid number is 2 to 6, and a molecular weight is 100 to 1200. The peptide and the fermented product using the soybean protein are contained, and the fermented product composition which is more excellent in collagen synthesis and cell proliferation can be obtained than the one containing only the fermented product.

The above peptides may be used alone or in combination of two or more. For example, the peptide may be used alone or in combination of two or more kinds. Further, the peptide may contain two or more different amino acids and/or molecular weights. Further, as the above-mentioned peptide, two or more kinds of peptides such as peptides obtained by hydrolysis of soybean protein and silk fibroin such as silk fibroin may be used in combination.

The above "vitamin A" is a compound having an isoprenoid type polyenol as a basic structure and a derivative of the compound. The above vitamin A type may specifically be, for example, vitamin A1 (retinol), vitamin A2, vitamin A3, and 3,4-dihydroretinol, and such oxidized aldehyde (retinal, etc.) and carboxylic acid. (retinoic acid) and the like. Further, the above "vitamin A" also includes a pharmaceutically acceptable salt and a pharmaceutically acceptable derivative. The above derivative may be, for example, a carboxylic acid ester such as the above retinol. More specifically, the above derivatives include C1 to C30 esters such as retinyl acetate and retinyl palmitate. Further, in the present invention, the above-mentioned vitamins may be used alone or in combination of two or more.

In the present invention, the form containing the above vitamin A is not particularly limited. The above-mentioned vitamin A may be contained in the ferment composition of the present invention itself, or may be contained together with other substances. For example, the above-mentioned vitamin A may be contained in a host compound such as cyclodextrin to form a compound, and it may be contained in the ferment composition of the present invention. Further, the above vitamin A may be a composition or a mixture containing the above vitamin A, and is contained in the ferment composition of the present invention. For example, the above-mentioned vitamin A may be contained in the ferment composition of the present invention as a composition containing the above vitamin A such as liver oil. Further, the above vitamin A may be contained in the ferment composition of the present invention as a solution or dispersion in which the above vitamin A is dissolved or dispersed. For example, the above vitamin A is dissolved in animal oil or vegetable oil, and the solution is contained in the ferment composition of the present invention.

In the fermented product composition of the present invention, the ratio between the fermented product and the peptide and the vitamin A is not particularly limited. When the total amount of the fermented product and the peptide and the vitamin A are 100% by mass, the ratio of the fermented product is usually 0.1 to 99.9% by mass, preferably 10 to 80% by mass, more preferably 40 to 60% by mass. %. In the present invention, the collagen synthesis action and the like can be improved by including one or more of the above fermented product, the peptide, and the vitamin A. In the present invention, when the fermented product does not greatly differ from the content of one or more of the peptide and the vitamin, that is, when the mass ratio of the fermented product is 40 to 60% by mass, collagen synthesis can be further improved. The effect is so good.

The ferment composition of the present invention can be used in various applications. This use is not particularly limited. The fermented product composition of the present invention can be used, for example, in cosmetics such as external preparations for skin, cosmetic raw materials, foods and drinks, food and drink additives, and bath essence.

The fermented product composition of the present invention can be blended with cosmetics, foods and drinks, and the like. When blended with a cosmetic, the compounding amount of the fermented product composition of the present invention is not particularly limited. The blending amount can be appropriately blended depending on the type of the cosmetic. When the amount of the composition of the fermented product of the present invention is 100% by mass of the cosmetic, it may be 0.001 to 100% by mass, particularly preferably 0.001 to 50% by mass. When the content of the composition of the fermented product of the present invention is within the above range, it can be a cosmetic having an excellent collagen synthesis action or the like.

The type of the cosmetic which can be combined with the ferment composition of the present invention is also not limited. The cosmetic can be, for example, freeze-dried, applied, coated, hardened, powdered, lotion, flour, solid powder, lipstick, solid blush, foundation cream, lotion, lotion, lotion, cleansing Liquid, facial cleanser, skin cream, moisturizer, hair lotion, eye shadow and eyebrow pencil. Moreover, such cosmetics may be added according to their type, such as plant extracts, vitamins, vitamins, minerals, lower alcohols, polyols, oils, surfactants, antioxidants, ultraviolet rays. Absorbents, tackifiers, pigments, preservatives and fragrances.

The cosmetic which is combined with the ferment composition of the present invention may especially be a skin external preparation. The ferment composition of the present invention has an excellent collagen synthesis effect. Therefore, the ferment composition of the present invention is particularly useful for use in skin-coated cosmetics. The external preparation for skin containing the ferment composition of the present invention as an active ingredient can be prepared by using the fermentate composition of the present invention and other compounding agents for external preparations for skin. The above other complexing agents may be, for example, liquid oils (squalane and jojoba oil), solid oils (beeswax and cetyl alcohol, etc.), various active agents, and humectants (glycerol and 1,3-butanediol, etc.) ). The dosage form of the external preparation for skin is not particularly limited. The external preparation for skin may be, for example, a lotion, a cream, an emulsion, or the like, and may be in various dosage forms depending on the purpose. The compounding amount of the fermented product composition of the present invention in the external preparation for skin is not particularly limited. The amount of the composition of the fermented product of the present invention is 0.001 to 100% by mass, particularly preferably 0.001 to 50% by mass, based on 100% by mass of the external preparation for skin. When the content of the composition of the fermented product of the present invention is within the above range, it can be an external preparation for skin having an excellent collagen synthesis action or the like.

The food and beverage system containing the fermentant composition of the present invention may, for example, be mixed with the other food material of the fermented product composition of the present invention, or may be blended with the fermented product composition of the present invention in a premixed food material mixture, and then The method of manufacturing. The food or drink containing the ferment composition of the present invention can be produced by dissolving the ferment composition of the present invention in, for example, fat, oil, ethanol, propylene glycol, glycerin or the like, and blending it with a beverage or a solid food. Further, in the food or drink containing the fermented product composition of the present invention, the fermented product composition of the present invention may be mixed with a binder such as gum arabic or dextrin, and then formed into a powder or granules, etc., and then blended into a beverage or a solid. Food can be made. The food or drink containing the ferment composition of the present invention can be provided as a health food, a functional food, and the like.

Further, the complex of the fermented product composition of the present invention can also be used as a raw material for cosmetics, a bathing agent, an additive for foods and drinks, and the like.

(2) Cosmetic composition, cell activating external skin agent composition and cosmetic raw material

The cosmetic composition, the skin external preparation for skin use, and the cosmetic raw material of the present invention are characterized by containing the fermentation product composition of the present invention. The composition of the present invention, the composition for external use of the skin for activating the skin, and the composition of the fermented product contained in the cosmetic raw material can be applied to the contents already described.

The ratio of the cosmetic composition of the present invention, the composition for exogenous skin for cell activating, and the composition of the above fermented material in the cosmetic raw material is not particularly limited. The ratio of the composition of the fermented product is, for example, 0.001 to 100% by mass, particularly 0.001 to 50% by mass, based on 100% by mass of the cosmetic composition, the external composition for cell activating agent, and the cosmetic raw material of the present invention. . When the ratio of the composition of the fermented product is within the above range, it can be used as a cosmetic composition having an excellent collagen synthesis action, a composition for external use for cell activating, and a cosmetic raw material.

The type of the cosmetic composition of the present invention is not particularly limited, and the cosmetic composition may be, for example, freeze-dried, coated, coated, hardened, flour, solid powder, lipstick, solid blush, foundation cream, lotion , lotion, lotion, cleansing lotion, face cream, skin cream, lotion, hair lotion, eye shadow and eyebrow pencil. The cosmetic composition of the present invention may also be added with other ingredients depending on the type (for example, plant extracts, vitamins, vitamin-like substances, minerals, lower alcohols, polyols, oils, surfactants, anti-drugs). Oxidizing agents, UV absorbers, tackifiers, colors, preservatives and fragrances).

More specifically, the cosmetic composition of the present invention is especially a skin external preparation. The above ferment composition has an excellent collagen synthesis effect. Therefore, the upper ferment composition is particularly useful for use in skin-coated cosmetics. The above-mentioned external preparation for skin can be prepared by using the above-mentioned ferment composition and other compounding agents used for the external preparation for skin. Other compounding agents may be, for example, liquid oils (squalane and jojoba oil), solid oils (beeswax and cetyl alcohol, etc.), various active agents, and humectants (glycerol and 1,3-butanediol, etc.) . The dosage form of the external preparation for skin is not particularly limited. The external preparation for skin may be a cosmetic liquid, a cream, an emulsion, or the like, and may be in various dosage forms depending on the purpose. In the external preparation for skin, the compounding amount of the above fermented product composition is not particularly limited. When the amount of the above-mentioned fermented product is 100% by mass of the external preparation for skin, it may be 0.001 to 100% by mass, and particularly preferably 0.001 to 50% by mass. When the content of the composition of the fermented product is within the above range, an external preparation for skin having an excellent collagen synthesis action or the like can be obtained.

The external agent for cell activating of the present invention is a composition containing the above fermented product. The above fermented product composition has excellent collagen synthesis action and the like. Therefore, the above ferment composition is most useful in blending with a cosmetic applied to the skin. The external preparation for cell activating of the present invention can be applied in accordance with the above description of the external preparation for skin.

The cosmetic raw material of the invention can be combined with freeze-drying, facial application, facial application, peeling, powder makeup, lotion, flour, solid powder, lipstick, solid blush, foundation cream, lotion, lotion, emollient Use liquid, cleaning solution, face cream, skin cream, moisturizer, hair lotion, eye shadow and eyebrow pencil.

(3) Method for producing fermented product composition and the like

The fermented product composition, the cosmetic composition, the cell exogenous skin external preparation composition, and the cosmetic raw material production method of the present invention are fermented substances and peptides and vitamin A obtained by fermenting peptides derived from rice bran and beans by Bacillus subtilis. The mixing step of at least one of the classes is characterized.

The above-mentioned "rice bran", the above-mentioned "peptide derived from beans", the above-mentioned "subtilis", the above-mentioned "peptide" and the above-mentioned "vitamin A" can be applied to the contents of the fermented product composition of the present invention.

The above-mentioned "fermentation product" is usually obtained by inoculating a Bacillus subtilis in a medium containing a peptide derived from rice bran and beans to ferment the fermentation step. The above-mentioned "medium" in the above "fermentation step" is a medium containing a peptide derived from rice bran and beans, which can be fermented and cultured by subtilizing bacteria. The kind and composition of the above medium are not particularly limited. The above medium is usually a liquid medium or a solid medium. Further, the pH of the medium (especially during fermentation) is usually 5.5 to 8.5, preferably 6.0 to 8.0, more preferably 6.5 to 7.5. In addition, the medium may be added with a sugar such as glucose, a protease or the like, a purified water or the like, which is adjusted to have a pH and a nutrient condition.

The culture temperature at the time of the above fermentation is not particularly limited as long as fermentation can be carried out. The culture temperature is usually 15 to 50 ° C, preferably 20 to 45 ° C, more preferably 30 to 45 ° C, and the optimum is 35 to 43 ° C. The fermentation culture system is usually carried out under aeration agitation.

The form of the fermented product obtained by the above fermentation step is also not particularly limited. The form of the above fermented product can be applied to the contents of the composition of the fermented product of the present invention.

In the above "mixing step", a method of mixing at least one of the above-mentioned fermented product and the above peptide and vitamin A is not particularly limited. The fermented product and the peptide may be mixed by a suitable device such as a homogenizer or the like by a respective form and a different mass ratio. Further, the temperature at the time of mixing is not particularly limited. It can be set according to various kinds of fermented substances and peptides. The fermented product and the peptide may be mixed at a temperature of 5 to 20 ° C, cooled as needed, and mixed while being heated at a temperature of 35 to 85 ° C depending on the need thereof.

The manufacturing method of the present invention may further comprise other steps. This other step may be, for example, a step of refining the above-mentioned fermented product or the like. The ferment obtained by the fermentation step may be directly mixed with the peptide, or the fermented product may be refined and then mixed with the peptide. This refining treatment is not particularly limited. For example, the fermented product can be purified by pressing the fermentation broth and filtering. The fermentation broth may also be purified by pressing the fermentation broth, filtering, and then deodorizing, decolorizing, and/or removing the precipitate by activated carbon, and then filtering again.

The step of preparing the composition of the fermented product to obtain a cosmetic composition and a composition for activating the skin external preparation is not particularly limited as long as the cosmetic composition and the composition for exogenous skin for activating the skin are available. In general, the above fermented product composition is mixed with other raw materials constituting the cosmetic composition and the cell activating external skin composition, and formulated into a tablet or a liquid preparation to obtain a cosmetic composition and a skin external preparation for cell activating. Composition. Needless to say, the above fermented product composition can be directly formulated into a tablet or a liquid preparation, and the like, and can also be a composition for a cosmetic composition and a skin external preparation for cell activating.

Example

The invention will be specifically described below by way of examples.

[1] A fermentation composition was prepared by mixing 3 kg of rice bran, 0.4 kg of soybean peptide, 0.5 kg of glucose, 0.01 kg of alkaline protease, and 50 kg of purified water to prepare a medium. Thereafter, the pH of the medium was adjusted to 6.0 to 8.0, followed by inoculating natto, and the mixture was cultured at 37 ° C for 38 hours for fermentation. The fermentation broth is then pressed and filtered, followed by decolorization and deodorization with activated carbon. Then, the fermentation broth was again filtered and purified to prepare a fermented product.

[2] Evaluation of the fermented product containing silk fibroin, the fermented product of the above [1], vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.) and silk fibroin (manufactured by Ordoreman Co., Ltd., trade name "Fibroinsheet"), respectively It was made to have a solid concentration of 2% (mass/volume) and dissolved in a buffer (PBS). It was then filtered through a 0.22 μm filter. Then, the solution was diluted, and the components were mixed at the concentrations shown in Tables 1 and 2 to prepare sample solutions of Experimental Examples 2 to 15. Further, in Experimental Example 1, the above buffer solution (PBS) was used as a sample solution.

In each of the two 96-well culture plates (referred to as "P1" and "P2" in the table), 5,000 human skin fibroblasts were sprinkled into each well. It was then cultured for 3 days in 5% FBS MEM medium. The samples of Experimental Examples 1 to 15 were added to the MEM medium to which 5% FBS was added, and the medium to which the sample was added was prepared. The concentration of the sample solution of Experimental Examples 1 to 15 in the medium to which the sample was added was 1% (mass/volume, except the mass of the above PBS), (so the final concentration of each component in the fermentation composition in the medium was sample 1/100 of the concentration in the solution). Then, the above 5% FBS in the well was added to the MEM medium to be replaced with the above-mentioned added sample medium, and culture was further carried out for 3 days.

Then, using the "Collagenstain set" (manufactured by the Collagen Technology Research Society), the amount of collagen synthesis (μg) was measured in accordance with the method of use attached to the kit. The average value of the amount of collagen synthesis in the disk 1 (P1) and the disk 2 (P2) is shown in Tables 1 and 2. Further, the amount of collagen synthesis in Experimental Example 1 was 1, and the relative value of the collagen synthesis amount in Experimental Examples 2 to 8 was determined. Similarly, the amount of collagen synthesis in Experimental Example 9 was 1, and the relative value of the collagen synthesis amount in Experimental Examples 10 to 15 was determined. The results are shown together in Tables 1 and 2. Further, "VC" in Tables 1 and 2 refers to vitamin C and "SF" which are silk fibroin.

As can be seen from Tables 1 and 2, Experimental Examples 5 to 8 and 13 to 15 in which silk fibroin and fermented materials were used together with Experimental Examples 3 and 11 in which fermented materials were used alone, and only experimental examples 4 and 12 of silk fibroin were used. Compared with collagen synthesis, it is better.

[3] Evaluation of the fermented product composition of the peptide containing the other raw materials of the silk fiber, the fermented product produced in the above [1], vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), silk fibroin and the following fibroin (a) And (b), dissolved in a buffer (PBS) to have a solid concentration of 2% (mass/volume). The solution was then filtered through a 0.22 [mu]m filter. Then, the components were diluted to the concentrations shown in Tables 3 to 5, and the solution was diluted and mixed to prepare sample solutions of Experimental Examples 17 to 19, 24 to 28, and 33 to 35. Further, the sample solutions of the experimental examples 17 to 19, 24 to 28, and 33 to 35 were mixed in the same volume to prepare the sample solutions of the experimental examples 20 to 22, 29 to 31, and 36. Further, in Experimental Examples 16, 23 and 32, the above buffer solution (PBS) was used as the sample solution.

The vitamin C and the silk fibroin are used in the same manner as the user in the above [2], and the silk fibroin (a) and (b) are used as follows. Further, the silk fibroin (a) and (b) were both liquid, and were diluted with a buffer to have a solid concentration of 1% (mass/volume) of the final concentration.

Silk fibroin (a): Cosmo Foods, Inc., trade name "Silk peptide M-500" silk fibroin (b): Cosmo Foods, Inc., trade name "Silkpeptide M-500 #60"

The amount of collagen synthesis (μg) of Experimental Examples 16 to 36 was measured in the same manner as in the above [2].

The average value of the amount of collagen synthesis in the culture disk 1 (P1) and the culture disk 2 (P2) is shown in Tables 3 to 5. Further, the amount of collagen synthesis in each of Experimental Examples 16, 23 and 32 was 1, and the relative value of the amount of collagen synthesis in the other experimental examples was determined. The results are also recorded in Tables 3 to 5. Further, in Tables 3 to 5, "VC" indicates vitamin C, "SF" shows silk fibroin, and "SP" shows silk fibroin.

From the results of Tables 3 to 5, it can be seen that the experimental examples 22, 29 and 36 in which the silk fibroin and the fermented product were used, and the experimental examples 18, 25 and 34 in which only the fermented product were used, and the experimental example 19 in which only fibroin was used, The 26 and 35 series have more excellent collagen synthesis effects as described above. Further, Experimental Examples 30 and 31 in which silk fibroin and fermented materials were used together had a superior collagen synthesis effect as silk fibroin. Further, as is apparent from Experimental Examples 17, 24 and 33, vitamin C also has a collagen synthesis action. However, in the experimental example 20 in which the fermented product and the vitamin C were used together with the experimental example 21 in which the vitamin C and the silk fibroin were used together, the synergistic effect was not seen, or only the effect was extremely small. Therefore, it can be seen that the synergistic effect of the fermented product and the peptide is large.

[4] Evaluation of the composition of the fermentation composition containing the soybean peptide, the fermented product of the above [1], the vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), and the solid component concentration of 2% (mass/volume) Fibrin and the following soybean peptides (a) to (c) were dissolved in buffer (PBS). The solution was then filtered through a 0.22 μm filter. Thereafter, each component was allowed to have the concentrations shown in Tables 6 and 7, and the solution was diluted and mixed. The sample solutions of Experimental Examples 38 to 43 and 49 to 54 were prepared. Further, the sample solutions of Experimental Examples 38 to 43 and 49 to 54 were mixed in the same volume, and the sample solutions of Experimental Examples 44 to 47 and 55 to 56 were prepared. Further, in Experimental Examples 37 and 48, the above buffer solution (PBS) was used as a sample solution.

Vitamin C and silk fibroin are used in the same manner as in [2] above. Further, the following peptides (a) to (c) are used. Further, the soybean peptides (a), (b), and (c) were all liquid, and were diluted in a buffer to have a final concentration of solid components of 1% (mass/volume).

Soy peptide (a): manufactured by Fuji Oil Co., Ltd., trade name "Hinute SMP" Soy peptide (b): manufactured by Fuji Oil Co., Ltd., trade name "Hinute R" soy peptide (c): Fuji Oil Company, trade name "Hinute DC5"

The amount of collagen synthesis (μg) of Experimental Examples 37 to 47 was measured in the same manner as in the above [2]. The average amount of collagen synthesis of the culture disk 1 (P1) and the culture disk 2 (P2) is shown in Table 6. Further, the relative value of the amount of collagen synthesis in the other experimental examples was determined by taking the amount of collagen synthesis in Experimental Example 37 as 1. The results are shown together in Table 6. In addition, in Table 6, "VC" shows vitamin C, "SF" shows silk fibroin, and "SEP (a) to (c)" shows soybean peptides (a) to (c).

In two 96-well culture dishes, 2000 human skin fibroblasts were sprinkled into each well. It was then cultured for 3 days in MEM medium supplemented with 5% FBS. It was then replaced with serum-free MEM medium and cultured for another day. The samples of the experimental examples 48 to 56 were added to the 0.5% serum MEM medium to prepare a medium to which the sample was added. The concentration of the sample solution of the above Experimental Examples 48 to 56 in the medium to which the sample was added was 1% (mass/volume, except the mass of the PBS), (so the final concentration of each component in the fermentation composition in the medium was in the sample solution. 1/100 of the concentration). Then, the above serum-free MEM medium in the well was changed to the above-mentioned medium to which the sample was added, and culture was further carried out for 4 days.

Then according to the "Cellcounting Kide8" of Tong Ren Tang, the WST-8 tetrazolium salt is used as the chromogenic substrate, and the aqueous solution is formed by the dehydrogenase reduction in living cells. The absorbance was measured at a wavelength of 450 nm (the number of viable cells is proportional to the value of the absorbance). Further, the number of viable cells in Experimental Example 48 was taken as 1, and the relative value of the amount of viable cells in the other experimental examples was calculated to evaluate the cell proliferation effect. The results are also shown in Table 7. Further, in Table 7, "VC" indicates vitamin C, "SF" indicates silk fibroin, and "SBP (a) to (c)" indicates soybean peptides (a) to (c). Further, the average value of the cell proliferation rate items in Table 7 is the average value of the above absorbance values.

From Table 6, it is understood that the experimental example 44 in which silk fibroin and the fermented product are used has a superior collagen synthesis effect than the experimental example 39 in which only the fermented product is used and the experimental example 40 in which only fibroin is used. In addition, the experimental examples 45 to 47 in which the fermented product and the soybean peptide were used together had the same superior collagen synthesis as the silk fibroin.

From Table 7, it is understood that the experimental example 56 in which the fermented product and the soybean peptide (c) are used together can further enhance the cell proliferation effect than the experimental example 50 in which only the fermented product is used. Further, in the same manner as in Experimental Example 55 in which the fermented product and the silk fibroin were used together, the cell proliferation effect was enhanced.

[5] Evaluation of the fermented product composition containing vitamin A, the fermented product produced in the above [1], vitamin C (manufactured by Wako Pure Chemical Industries, Ltd.), and cyclodextrin containing retinol (trade name "CAVAMAX W8/" Retinol Complex "made by Cyclokem Co., Ltd.) can be dissolved in a buffer (PBS) at a solid concentration of 2% (mass/volume). The solution was then filtered through a 0.22 [mu]m filter. Then, the solution was diluted so that the components were mixed at the concentrations shown in Table 8, and the sample solutions of Experimental Examples 58 to 66 were prepared. Further, in Experimental Example 57, the above buffer solution (PBS) was used as a sample solution.

The collagen synthesis amount (μg) and the cell proliferation rate of Experimental Examples 57 to 66 were measured in the same manner as in the above [2] and [4]. The average value is shown in Table 8. The amount of collagen synthesis and the amount of viable cells in the other experimental examples were determined by the amount of collagen synthesis and the amount of viable cells in Experimental Example 57. The results are shown in Table 8. Further, in Table 8, "VC" indicates vitamin C, and "CDR" indicates that cyclodextrin contains vitamin A. Further, the average ratio of the cell proliferation rate items in Table 8 is the average value of the absorbance values.

It can be seen from Table 8 that the experimental examples 64 to 66 using vitamin A and the fermented product have superior collagen synthesis and cell proliferation effects than the experimental example 59 using only the fermented product and the experimental examples 60 to 63 using only the vitamin A. .

[6] The fermented product composition containing the peptide and the vitamin A is evaluated to dissolve the fermented product produced in the above [1], the silk fibroin, the soybean peptide (a) and the cyclodextrin containing vitamin A in a buffer (PBS) ), respectively, to a solid concentration of 2% (mass / volume). The solution was then filtered through a 0.22 [mu]m filter. Then, the solution was diluted, and the components were mixed at the concentrations shown in Tables 9 and 10 to prepare sample solutions of Experimental Examples 68, 69, 71, and 72. Further, in Experimental Examples 67 and 70, the above buffer solution (PBS) was used as the sample solution.

The collagen synthesis amount (μg) and the cell proliferation rate of the experimental examples 67 to 72 were measured in the same manner as in the above [2] and [4]. The average value is shown in Table 9 and Table 10. Further, the collagen synthesis amount and the viable cell amount of the experimental examples 67 and 70 were 1, and the relative values of the collagen synthesis amount and the viable cell amount of the other experimental examples were determined. The results are also shown in Tables 9 and 10. Further, in Tables 9 and 10, "SF" indicates silk fibroin, "SBP" indicates soybean peptide, and "CDR" indicates that cyclodextrin contains vitamin A. Further, the average values of the items of the cell proliferation rate in Tables 9 and 10 are the average values of the absorbance values.

As is clear from Table 10, the experimental examples 69 and 72 in which vitamin A and peptides and the fermented product were used together had excellent collagen synthesis and cell proliferation.

Further, the present invention is not limited to the specific embodiments described above. The present invention can be variously modified within the scope of the present invention in accordance with the purpose and use.

[Industrial availability]

The ferment composition of the present invention has excellent safety and is a collagen synthesis. The fermented product composition of the present invention can be used in various cosmetics such as skin external preparations, various foods and drinks such as health foods and functional foods, and the like. That is, the present invention can be utilized in fields such as cosmetics and foods and drinks.

Claims (6)

A fermented product comprising a fermented product obtained by fermenting rice bran and a peptide derived from beans by a subtilis, and a peptide derived from at least one of the peptides and the vitamin A, characterized by a peptide-derived soybean peptide derived from the above-mentioned legumes, In the above fermentation, the pH of the medium is 5.5 to 8.5, the culture temperature is 15 to 50 ° C, and the peptide is at least one of a peptide hydrolyzed by soybean protein and silk fibroin, and the vitamin A is a cyclodextrin. The compounding amount of the above-mentioned peptide is 9:1 to 1:9, and the amount of the above-mentioned vitamin A is 0.1 to 1 with respect to 100 parts by mass of the above fermented product. Parts by mass. A cosmetic composition characterized by containing the ferment composition of claim 1 of the patent application. A method for producing a composition for a fermented product, comprising the steps of mixing a fermented product obtained by fermenting rice bran and a peptide derived from beans with at least one peptide and vitamin A, and is characterized by being derived from the above-mentioned beans The peptide system is a soybean peptide. In the above fermentation, the pH of the medium is 5.5-8.5, the culture temperature is 15-50 ° C, and the peptide is the peptide of soybean protein hydrolyzed and silk fibroin. One type of vitamin A, the cyclodextrin contains retinol, and the amount of the above peptide is such that the mass ratio of the fermented product to the peptide is 9:1 to 1:9, and the amount of the vitamin A is combined. The amount is 0.1 to 1 part by mass based on 100 parts by mass of the fermented product. A method for producing a cosmetic composition comprising the steps of mixing a fermented product obtained by fermenting rice bran and a bean-derived peptide with at least one peptide and vitamin A, and formulating the fermented product obtained in the mixing step The peptide obtained from the above-mentioned legumes is a soybean peptide, and the pH of the medium is 5.5 to 8.5, the culture temperature is 15 to 50 ° C, and the above peptide is soybean. At least one of a protein hydrolyzed peptide and a silk fibroin, wherein the vitamin A, the cyclodextrin comprises retinol, and the compounding amount of the peptide is 9:1 of the fermented product and the peptide. 1:9, the amount of the vitamin A to be added is 0.1 to 1 part by mass based on 100 parts by mass of the fermented product. A composition for activating a skin external preparation comprising a step of mixing a fermented product obtained by fermenting rice bran with a bean-derived peptide with at least one peptide and vitamin A; And the step of formulating the fermented product composition obtained in the mixing step to obtain a cosmetic composition, wherein the peptide derived from the soybean is a soybean peptide, and the peptide is a peptide hydrolyzed by soybean protein and silk fibroin. In at least one of the above vitamin A, the cyclodextrin comprises retinol, and the compounding amount of the peptide is a mass ratio of the fermented product to the peptide of 9:1 to 1:9, and the vitamin A type The blending amount is 0.1 to 1 part by mass based on 100 parts by mass of the above-mentioned fermented product. A method for producing a composition for activating a skin external preparation, which comprises mixing a fermented product obtained by fermenting rice bran with a bean and a peptide derived from a bean, and at least one peptide and vitamin A, and mixing the preparation with the preparation The step of obtaining the fermented product composition in the step of obtaining a cosmetic composition is characterized in that the peptide derived from the above-mentioned legume is soybean peptide, and the pH of the medium is 5.5 to 8.5 and the culture temperature is 15 to 50 ° C in the above fermentation. The peptide is at least one of a peptide hydrolyzed by soybean protein and a silk fibroin, and the vitamin A is a cyclodextrin containing retinol, and the amount of the peptide is the mass of the fermented product and the peptide. The ratio of the above-mentioned vitamin A is 9:1 to 1:9, which is relative to the above-mentioned fermented product 100. The mass fraction is 0.1 to 1 part by mass.