WO2006095964A1 - Procede d'extraction d'un extrait liquide a partir d'une chlorelle - Google Patents

Procede d'extraction d'un extrait liquide a partir d'une chlorelle Download PDF

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Publication number
WO2006095964A1
WO2006095964A1 PCT/KR2006/000462 KR2006000462W WO2006095964A1 WO 2006095964 A1 WO2006095964 A1 WO 2006095964A1 KR 2006000462 W KR2006000462 W KR 2006000462W WO 2006095964 A1 WO2006095964 A1 WO 2006095964A1
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WO
WIPO (PCT)
Prior art keywords
chlorella
beneficial substance
heating
extract
cell membrane
Prior art date
Application number
PCT/KR2006/000462
Other languages
English (en)
Inventor
Hyun Jin Jin
Original Assignee
Hyun Jin Jin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyun Jin Jin filed Critical Hyun Jin Jin
Publication of WO2006095964A1 publication Critical patent/WO2006095964A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60—Edible seaweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/202—Algae extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/14—Extraction
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/24—Heat, thermal treatment
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/31—Mechanical treatment

Definitions

  • the present invention relates to a method for extracting a liquid extract from chlorella, and more particularly to a method for extracting a liquid extract from chlorella, comprising heating chlorella to isolate the chlorella cells and extracting from the cells only a beneficial substance in which nutrients, such as amino acids, fats, proteins, vitamins and saccharides, are abundantly contained in the hard cell membrane, an insoluble substance.
  • chlorella contains rich nutrients, such as amino acids, fats, proteins and saccharides, but upon its ingestion, the rich nutrients contained in the cell membrane are not easily absorbed into the human body, since the cell membrane of chlorella is very hard.
  • chlorella is processed into powder by disrupting the cell membrane.
  • the nutrients contained in chlorella cannot be sufficiently absorbed into the body, since the cell wall and cell membrane of chlorella are not sufficiently disrupted.
  • chlorella extract is a hot water extract obtained by removing an insoluble substance from chlorella cells and then concentrating the remaining beneficial substance or drying the beneficial substance into powder and contains amino acids, proteins, peptides, saccharides, vitamins and minerals.
  • the chlorella proteins contain nucleic acids known as the main components of a chlorella growth factor (hereinafter, referred to as "CGF ”) having a physiological activity of stimulating growth to make the skin younger.
  • CGF chlorella growth factor
  • the liquid extract from chlorella is processed into a functional beverage which is directly drinkable, or it is used as the raw material of food additives.
  • the CFG is a specific substance which is contained only in chlorella, and it is not contained in other higher plants.
  • the CGF in the chlorella extract contains, as a main component, a S-nucleotide-adenosyl peptide complex having a molecular weight of about 5,000-10,000.
  • the content of the CGF is expressed as optical density (OD) per g of an extract, which is measured at a UV wavelength of 260 nm, which is the peak absorption wavelength of nucleic acids, such as DNA and RNA, the main components of the CGF. Disclosure of Invention Technical Problem
  • a general method for extracting a liquid extract from chlorella is a method as shown in FIG. 1, which comprises adding hot water of 90 0 C to chlorella powder so as to extract from the chlorella only a beneficial substance including nutrients abundantly contained in the cell membrane, and then liquefying the beneficial substance.
  • this method has a problem in that unnecessary processes are repeated, since a crude culture medium having chlorella growth therein is powdered and the powder is dissolved in water to make a crude liquid containing chlorella dissolved therein.
  • Another problem is that the extraction process is very inefficient, since the method requires an additional process, such as adjusting an aqueous solution of chlorella powder to an acidic pH range in order to prevent the precipitation of the chlorella powder in the aqueous solution.
  • the present invention has been made to solve the above-described problems occurring in the prior art, and it is an object of the present invention to provide a method for extracting a liquid extract from chlorella, which can eliminate a heating process of powdering chlorella and a process of dissolving the chlorella powder in water, because the chlorella liquid extract is extracted directly from a crude culture medium having chlorella grown therein, unlike the prior method of extracting a chlorella extract by dissolving chlorella powder in water, and which can eliminate a process of adjusting the pH of an aqueous solution of chlorella powder, because only the beneficial substance is extracted directly from the crude culture medium having chlorella grown therein, and thus there is no occurrence of precipitates in the liquid extract.
  • the chlorella liquid extract is extracted directly from the crude culture medium having chlorella grown therein, unlike the prior method of extracting a chlorella extract by dissolving chlorella powder in water.
  • the inventive extraction method is performed in a very simple manner.
  • the liquid extract prepared according to the present invention contains a rich amount of beneficial nutrients.
  • FIG. 1 is a flow diagram showing a preparation process of extracting and liquefying a chlorella extract according to the prior method.
  • FIG. 2 is a flow diagram showing a preparation process of extracting a liquid extract according to the present invention.
  • the present invention relates to a method of extracting a liquid extract from chlorella, and more particularly to a method of extracting a liquid extract from chlorella, comprising heating chlorella so as to separate the chlorella cell into an insoluble hard cell membrane and a content filled in the cell membrane, and extracting from the content only a beneficial substance rich in nutrients, such as amino acids, fats, proteins, vitamins and saccharides.
  • the inventive method of extracting the liquid extract from chlorella comprises:
  • the crude culture medium having chlorella grown therein has a chlorella cell number of 10,000,000- 15,000,000 cells/500 ml. If the number of chlorella cells is lower than the lower limit of the above-specified range, the content of various nutrients in the liquid extract can be insufficient, because the chlorella culture medium is not sufficiently concentrated, and if the number of chlorella cells exceeds the above range, an unnecessarily excessive amount of various nutrients can be contained in the liquid extract, because the chlorella culture medium is excessively concentrated to saturation.
  • the crude culture medium is heated at a temperature of 120 + 5 0 C for 15-20 minutes so as to extract a content containing a beneficial substance rich in nutrients, such as amino acids, fats, proteins, vitamins and saccharides, in the chlorella cell membrane.
  • nutrients such as amino acids, fats, proteins, vitamins and saccharides
  • the content separated in the heating/extraction step is rotated at a speed of 6,000 rpm for 8-10 minutes by means of a centrifuge so as to separate the content into a hard cell membrane and a beneficial substance at the lower and upper portions, respectively.
  • the volume ratio of the separated cell membrane to the beneficial substance is about 10-15:85:90.
  • a filter membrane having a pore size of 0.5-1 D In the filtration step iii), only the beneficial substance separated in the cell separation step is collected, and purified by passing it through a filter membrane having a pore size of 0.5-1 D.
  • a microfibrous material the shell of chlorella
  • a cell wall and a cell membrane are filtered out through the filter so as to remove various impurities, such as hazardous components and toxin, while a nutrient substance beneficial for the human body are passed through the filter.
  • the passed nutrient substance is named "chlorella extract ".
  • the beneficial substance passed through the filtration step is sterilized by heating at a temperature of 120 + 5 0 C for 15-20 minutes.
  • Each of three aqueous solutions of chlorella powder was prepared by dissolving 100 g of chlorella powder in 900 g of water. To each of the aqueous solutions, hot water of 90 0 C was added for about 30 minutes so as to isolate a content in the chlorella cell membrane. The isolated content was rotated at a speed of 6,000 rpm for 10 minutes by means of a centrifuge so as to separate it into the cell membrane and a beneficial substance. The separated beneficial substance was deproteinated to remove precipitates and then purified by passing it through a filter membrane having a pore size of 1 D. Then, the filtrate was adjusted to a pH of 4.5-5.0 in order to prevent the chlorella powder from being precipitated in the aqueous extract solution. Then, the resulting substance was sterilized by heating at a temperature of 120 0 C for 20 minutes.
  • the present invention provides the method of extracting the liquid extract from chlorella, comprising heating chlorella to isolate chlorella cells and then extracting from the chlorella cells only a beneficial substance in which nutrients, such as amino acids, fats, proteins, vitamins and saccharides, are abundantly contained in the hard cell membrane, an insoluble substance.
  • the chlorella liquid extract is extracted directly from the crude culture medium having chlorella grown therein, unlike the prior method of extracting the chlorella extract by dissolving chlorella powder in water. Accordingly, some processes are greatly eliminated, and so the extraction method according to the present invention is performed in a very simple manner.
  • the liquid extract according to the present invention contains a rich amount of beneficial nutrients.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

L'invention concerne un procédé d'extraction d'un extrait liquide directement à partir d'un milieu de culture de brut contenant une chlorelle, à la différence du procédé usuel d'extraction d'extrait de chlorelle par dissolution de poudre de chlorelle dans l'eau. Le procédé de cette invention comprend les étapes suivantes: chauffage d'un milieu de culture de brut contenant une chlorelle de manière à extraire un liquide contenant des nutriments dans la membrane cellulaire de la chlorelle; séparation du liquide extrait lors du chauffage/extraction en une membrane cellulaire insoluble et une substance bénéfique au moyen d'une centrifugeuse; filtration de la substance bénéfique séparée lors de la séparation cellulaire; et stérilisation de la substance bénéfique provenant de la filtration.
PCT/KR2006/000462 2005-03-08 2006-02-09 Procede d'extraction d'un extrait liquide a partir d'une chlorelle WO2006095964A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2005-0018941 2005-03-08
KR1020050018941A KR100657637B1 (ko) 2005-03-08 2005-03-08 클로렐라로부터 액상 추출물을 추출하는 방법

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WO2006095964A1 true WO2006095964A1 (fr) 2006-09-14

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KR (1) KR100657637B1 (fr)
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Cited By (22)

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WO2011013707A1 (fr) 2009-07-29 2011-02-03 味の素株式会社 Procédé de production d'un acide l-aminé
WO2011127160A1 (fr) * 2010-04-06 2011-10-13 Heliae Development, Llc Extraction sélective de protéines présentes dans des algues poussant en eau douce
US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
US8157994B2 (en) 2010-04-06 2012-04-17 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of oil and co-products from oleaginous material
USD661164S1 (en) 2011-06-10 2012-06-05 Heliae Development, Llc Aquaculture vessel
US8202425B2 (en) 2010-04-06 2012-06-19 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8211309B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of proteins by a two solvent method
US8211308B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of polar lipids by a two solvent method
US8273248B1 (en) 2010-04-06 2012-09-25 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
US8341877B2 (en) 2011-05-31 2013-01-01 Heliae Development, Llc Operation and control of V-trough photobioreactor systems
USD679965S1 (en) 2011-06-10 2013-04-16 Heliae Development, Llc Aquaculture vessel
USD682637S1 (en) 2011-06-10 2013-05-21 Heliae Development, Llc Aquaculture vessel
US8475660B2 (en) 2010-04-06 2013-07-02 Heliae Development, Llc Extraction of polar lipids by a two solvent method
WO2015140467A1 (fr) * 2014-03-18 2015-09-24 Roquette Freres Procédé de perméabilisation thermique d'une biomasse de microalgues
US9200236B2 (en) 2011-11-17 2015-12-01 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
CN106488927A (zh) * 2014-07-18 2017-03-08 罗盖特公司 用于从微藻生物质提取可溶性蛋白的方法
CN107208124A (zh) * 2015-01-26 2017-09-26 罗盖特兄弟公司 用于分级富含蛋白质微藻的生物质的组分的方法
CN107217010A (zh) * 2016-09-01 2017-09-29 刘翔 一种液体培养冠突散囊菌的菌液分离方法
EP3403662A1 (fr) * 2017-05-02 2018-11-21 National Institute of Ocean Technology Nutriment pharmacoactif et son procédé de production à partir d'algues marines
US10519204B2 (en) 2014-07-18 2019-12-31 Corbion Biotech, Inc. Method for extracting soluble proteins from microalgal biomass

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KR100983023B1 (ko) 2010-07-07 2010-09-17 한국해양연구원 부등편모조류 또는 착편모조류에 속하는 미세조류의 지방산으로부터 트리글리세라이드 또는 지방산메틸에스테르를 추출하는 방법 및 이를 이용한 바이오디젤 제조방법
WO2012081931A2 (fr) * 2010-12-17 2012-06-21 Kim Sung-Chun Procédé et appareil utilisables en vue de la production de cellules et de matériaux solubles dans les graisses par culture de cellules

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JPS6158552A (ja) * 1984-08-31 1986-03-25 Nisshin Oil Mills Ltd:The 新規調味料
JPH0975094A (ja) * 1995-09-14 1997-03-25 Japan Kurorera Konsaruteeshiyon:Kk クロレラ藻体由来の緑色抽出液とその製造法
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Cited By (75)

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Publication number Priority date Publication date Assignee Title
WO2011013707A1 (fr) 2009-07-29 2011-02-03 味の素株式会社 Procédé de production d'un acide l-aminé
CN102834021A (zh) * 2010-04-06 2012-12-19 赫里开发公司 从盐水藻类选择性提取蛋白质
US8741629B2 (en) 2010-04-06 2014-06-03 Heliae Development, Llc Selective heated extraction of globulin proteins from intact freshwater algal cells
US8084038B2 (en) 2010-04-06 2011-12-27 Heliae Development, Llc Methods of and systems for isolating nutraceutical products from algae
US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
US20120053323A1 (en) * 2010-04-06 2012-03-01 Heliae Development, Llc Selective heated extraction of albumin proteins from intact freshwater algal cells
US8137555B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Methods of and systems for producing biofuels
US8137556B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Methods of producing biofuels from an algal biomass
US8137558B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Stepwise extraction of plant biomass for diesel blend stock production
US8142659B2 (en) 2010-04-06 2012-03-27 Heliae Development, LLC. Extraction with fractionation of oil and proteinaceous material from oleaginous material
US8152870B2 (en) 2010-04-06 2012-04-10 Heliae Development, Llc Methods of and systems for producing biofuels
US8153137B2 (en) 2010-04-06 2012-04-10 Heliae Development, Llc Methods of and systems for isolating carotenoids and omega-3 rich oil products from algae
US8157994B2 (en) 2010-04-06 2012-04-17 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of oil and co-products from oleaginous material
US8182689B2 (en) 2010-04-06 2012-05-22 Heliae Development, Llc Methods of and systems for dewatering algae and recycling water therefrom
US8182556B2 (en) 2010-04-06 2012-05-22 Haliae Development, LLC Liquid fractionation method for producing biofuels
US8187463B2 (en) 2010-04-06 2012-05-29 Heliae Development, Llc Methods for dewatering wet algal cell cultures
US8197691B2 (en) 2010-04-06 2012-06-12 Heliae Development, Llc Methods of selective removal of products from an algal biomass
US8202425B2 (en) 2010-04-06 2012-06-19 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8211309B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of proteins by a two solvent method
US8212060B2 (en) 2010-04-06 2012-07-03 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of oil and co-products from oleaginous material
US8211308B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of polar lipids by a two solvent method
US8222437B2 (en) 2010-04-06 2012-07-17 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction of lipids from oleaginous material
US8242296B2 (en) 2010-04-06 2012-08-14 Heliae Development, Llc Products from step-wise extraction of algal biomasses
US8273248B1 (en) 2010-04-06 2012-09-25 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8293108B1 (en) 2010-04-06 2012-10-23 Heliae Developmet, LLC Methods of and systems for producing diesel blend stocks
US8308950B2 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of dewatering algae for diesel blend stock production
US8308948B2 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of selective extraction and fractionation of algal products
US8308949B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of extracting neutral lipids and producing biofuels
US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
US8313647B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Nondisruptive methods of extracting algal components for production of carotenoids, omega-3 fatty acids and biofuels
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
US8318963B2 (en) 2010-04-06 2012-11-27 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of lipids and co-products from oleaginous material
US8318019B2 (en) 2010-04-06 2012-11-27 Heliae Development, Llc Methods of dewatering algae for extraction of algal products
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