WO2006095964A1 - Procede d'extraction d'un extrait liquide a partir d'une chlorelle - Google Patents
Procede d'extraction d'un extrait liquide a partir d'une chlorelle Download PDFInfo
- Publication number
- WO2006095964A1 WO2006095964A1 PCT/KR2006/000462 KR2006000462W WO2006095964A1 WO 2006095964 A1 WO2006095964 A1 WO 2006095964A1 KR 2006000462 W KR2006000462 W KR 2006000462W WO 2006095964 A1 WO2006095964 A1 WO 2006095964A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- chlorella
- beneficial substance
- heating
- extract
- cell membrane
- Prior art date
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 83
- 239000000284 extract Substances 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 title claims abstract description 27
- 239000000126 substance Substances 0.000 claims abstract description 36
- 230000009286 beneficial effect Effects 0.000 claims abstract description 32
- 210000000170 cell membrane Anatomy 0.000 claims abstract description 24
- 235000015097 nutrients Nutrition 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 150000001720 carbohydrates Chemical class 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 239000003925 fat Substances 0.000 description 5
- 235000013343 vitamin Nutrition 0.000 description 5
- 229940088594 vitamin Drugs 0.000 description 5
- 229930003231 vitamin Natural products 0.000 description 5
- 239000011782 vitamin Substances 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 4
- 235000019197 fats Nutrition 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- -1 DNA and RNA Chemical class 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000020510 functional beverage Nutrition 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000008729 phenylalanine Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000007686 potassium Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/202—Algae extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/24—Heat, thermal treatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/31—Mechanical treatment
Definitions
- the present invention relates to a method for extracting a liquid extract from chlorella, and more particularly to a method for extracting a liquid extract from chlorella, comprising heating chlorella to isolate the chlorella cells and extracting from the cells only a beneficial substance in which nutrients, such as amino acids, fats, proteins, vitamins and saccharides, are abundantly contained in the hard cell membrane, an insoluble substance.
- chlorella contains rich nutrients, such as amino acids, fats, proteins and saccharides, but upon its ingestion, the rich nutrients contained in the cell membrane are not easily absorbed into the human body, since the cell membrane of chlorella is very hard.
- chlorella is processed into powder by disrupting the cell membrane.
- the nutrients contained in chlorella cannot be sufficiently absorbed into the body, since the cell wall and cell membrane of chlorella are not sufficiently disrupted.
- chlorella extract is a hot water extract obtained by removing an insoluble substance from chlorella cells and then concentrating the remaining beneficial substance or drying the beneficial substance into powder and contains amino acids, proteins, peptides, saccharides, vitamins and minerals.
- the chlorella proteins contain nucleic acids known as the main components of a chlorella growth factor (hereinafter, referred to as "CGF ”) having a physiological activity of stimulating growth to make the skin younger.
- CGF chlorella growth factor
- the liquid extract from chlorella is processed into a functional beverage which is directly drinkable, or it is used as the raw material of food additives.
- the CFG is a specific substance which is contained only in chlorella, and it is not contained in other higher plants.
- the CGF in the chlorella extract contains, as a main component, a S-nucleotide-adenosyl peptide complex having a molecular weight of about 5,000-10,000.
- the content of the CGF is expressed as optical density (OD) per g of an extract, which is measured at a UV wavelength of 260 nm, which is the peak absorption wavelength of nucleic acids, such as DNA and RNA, the main components of the CGF. Disclosure of Invention Technical Problem
- a general method for extracting a liquid extract from chlorella is a method as shown in FIG. 1, which comprises adding hot water of 90 0 C to chlorella powder so as to extract from the chlorella only a beneficial substance including nutrients abundantly contained in the cell membrane, and then liquefying the beneficial substance.
- this method has a problem in that unnecessary processes are repeated, since a crude culture medium having chlorella growth therein is powdered and the powder is dissolved in water to make a crude liquid containing chlorella dissolved therein.
- Another problem is that the extraction process is very inefficient, since the method requires an additional process, such as adjusting an aqueous solution of chlorella powder to an acidic pH range in order to prevent the precipitation of the chlorella powder in the aqueous solution.
- the present invention has been made to solve the above-described problems occurring in the prior art, and it is an object of the present invention to provide a method for extracting a liquid extract from chlorella, which can eliminate a heating process of powdering chlorella and a process of dissolving the chlorella powder in water, because the chlorella liquid extract is extracted directly from a crude culture medium having chlorella grown therein, unlike the prior method of extracting a chlorella extract by dissolving chlorella powder in water, and which can eliminate a process of adjusting the pH of an aqueous solution of chlorella powder, because only the beneficial substance is extracted directly from the crude culture medium having chlorella grown therein, and thus there is no occurrence of precipitates in the liquid extract.
- the chlorella liquid extract is extracted directly from the crude culture medium having chlorella grown therein, unlike the prior method of extracting a chlorella extract by dissolving chlorella powder in water.
- the inventive extraction method is performed in a very simple manner.
- the liquid extract prepared according to the present invention contains a rich amount of beneficial nutrients.
- FIG. 1 is a flow diagram showing a preparation process of extracting and liquefying a chlorella extract according to the prior method.
- FIG. 2 is a flow diagram showing a preparation process of extracting a liquid extract according to the present invention.
- the present invention relates to a method of extracting a liquid extract from chlorella, and more particularly to a method of extracting a liquid extract from chlorella, comprising heating chlorella so as to separate the chlorella cell into an insoluble hard cell membrane and a content filled in the cell membrane, and extracting from the content only a beneficial substance rich in nutrients, such as amino acids, fats, proteins, vitamins and saccharides.
- the inventive method of extracting the liquid extract from chlorella comprises:
- the crude culture medium having chlorella grown therein has a chlorella cell number of 10,000,000- 15,000,000 cells/500 ml. If the number of chlorella cells is lower than the lower limit of the above-specified range, the content of various nutrients in the liquid extract can be insufficient, because the chlorella culture medium is not sufficiently concentrated, and if the number of chlorella cells exceeds the above range, an unnecessarily excessive amount of various nutrients can be contained in the liquid extract, because the chlorella culture medium is excessively concentrated to saturation.
- the crude culture medium is heated at a temperature of 120 + 5 0 C for 15-20 minutes so as to extract a content containing a beneficial substance rich in nutrients, such as amino acids, fats, proteins, vitamins and saccharides, in the chlorella cell membrane.
- nutrients such as amino acids, fats, proteins, vitamins and saccharides
- the content separated in the heating/extraction step is rotated at a speed of 6,000 rpm for 8-10 minutes by means of a centrifuge so as to separate the content into a hard cell membrane and a beneficial substance at the lower and upper portions, respectively.
- the volume ratio of the separated cell membrane to the beneficial substance is about 10-15:85:90.
- a filter membrane having a pore size of 0.5-1 D In the filtration step iii), only the beneficial substance separated in the cell separation step is collected, and purified by passing it through a filter membrane having a pore size of 0.5-1 D.
- a microfibrous material the shell of chlorella
- a cell wall and a cell membrane are filtered out through the filter so as to remove various impurities, such as hazardous components and toxin, while a nutrient substance beneficial for the human body are passed through the filter.
- the passed nutrient substance is named "chlorella extract ".
- the beneficial substance passed through the filtration step is sterilized by heating at a temperature of 120 + 5 0 C for 15-20 minutes.
- Each of three aqueous solutions of chlorella powder was prepared by dissolving 100 g of chlorella powder in 900 g of water. To each of the aqueous solutions, hot water of 90 0 C was added for about 30 minutes so as to isolate a content in the chlorella cell membrane. The isolated content was rotated at a speed of 6,000 rpm for 10 minutes by means of a centrifuge so as to separate it into the cell membrane and a beneficial substance. The separated beneficial substance was deproteinated to remove precipitates and then purified by passing it through a filter membrane having a pore size of 1 D. Then, the filtrate was adjusted to a pH of 4.5-5.0 in order to prevent the chlorella powder from being precipitated in the aqueous extract solution. Then, the resulting substance was sterilized by heating at a temperature of 120 0 C for 20 minutes.
- the present invention provides the method of extracting the liquid extract from chlorella, comprising heating chlorella to isolate chlorella cells and then extracting from the chlorella cells only a beneficial substance in which nutrients, such as amino acids, fats, proteins, vitamins and saccharides, are abundantly contained in the hard cell membrane, an insoluble substance.
- the chlorella liquid extract is extracted directly from the crude culture medium having chlorella grown therein, unlike the prior method of extracting the chlorella extract by dissolving chlorella powder in water. Accordingly, some processes are greatly eliminated, and so the extraction method according to the present invention is performed in a very simple manner.
- the liquid extract according to the present invention contains a rich amount of beneficial nutrients.
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- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Zoology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
L'invention concerne un procédé d'extraction d'un extrait liquide directement à partir d'un milieu de culture de brut contenant une chlorelle, à la différence du procédé usuel d'extraction d'extrait de chlorelle par dissolution de poudre de chlorelle dans l'eau. Le procédé de cette invention comprend les étapes suivantes: chauffage d'un milieu de culture de brut contenant une chlorelle de manière à extraire un liquide contenant des nutriments dans la membrane cellulaire de la chlorelle; séparation du liquide extrait lors du chauffage/extraction en une membrane cellulaire insoluble et une substance bénéfique au moyen d'une centrifugeuse; filtration de la substance bénéfique séparée lors de la séparation cellulaire; et stérilisation de la substance bénéfique provenant de la filtration.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050018941A KR100657637B1 (ko) | 2005-03-08 | 2005-03-08 | 클로렐라로부터 액상 추출물을 추출하는 방법 |
KR10-2005-0018941 | 2005-03-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2006095964A1 true WO2006095964A1 (fr) | 2006-09-14 |
Family
ID=36953534
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2006/000462 WO2006095964A1 (fr) | 2005-03-08 | 2006-02-09 | Procede d'extraction d'un extrait liquide a partir d'une chlorelle |
Country Status (2)
Country | Link |
---|---|
KR (1) | KR100657637B1 (fr) |
WO (1) | WO2006095964A1 (fr) |
Cited By (22)
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WO2011013707A1 (fr) | 2009-07-29 | 2011-02-03 | 味の素株式会社 | Procédé de production d'un acide l-aminé |
WO2011127161A1 (fr) * | 2010-04-06 | 2011-10-13 | Heliae Development, Llc | Extraction sélective de protéines présentes dans les algues poussant en eau salée |
US8115022B2 (en) | 2010-04-06 | 2012-02-14 | Heliae Development, Llc | Methods of producing biofuels, chlorophylls and carotenoids |
US8157994B2 (en) | 2010-04-06 | 2012-04-17 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Extraction with fractionation of oil and co-products from oleaginous material |
USD661164S1 (en) | 2011-06-10 | 2012-06-05 | Heliae Development, Llc | Aquaculture vessel |
US8202425B2 (en) | 2010-04-06 | 2012-06-19 | Heliae Development, Llc | Extraction of neutral lipids by a two solvent method |
US8211309B2 (en) | 2010-04-06 | 2012-07-03 | Heliae Development, Llc | Extraction of proteins by a two solvent method |
US8211308B2 (en) | 2010-04-06 | 2012-07-03 | Heliae Development, Llc | Extraction of polar lipids by a two solvent method |
US8273248B1 (en) | 2010-04-06 | 2012-09-25 | Heliae Development, Llc | Extraction of neutral lipids by a two solvent method |
US8308951B1 (en) | 2010-04-06 | 2012-11-13 | Heliae Development, Llc | Extraction of proteins by a two solvent method |
US8313648B2 (en) | 2010-04-06 | 2012-11-20 | Heliae Development, Llc | Methods of and systems for producing biofuels from algal oil |
US8341877B2 (en) | 2011-05-31 | 2013-01-01 | Heliae Development, Llc | Operation and control of V-trough photobioreactor systems |
USD679965S1 (en) | 2011-06-10 | 2013-04-16 | Heliae Development, Llc | Aquaculture vessel |
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US8475660B2 (en) | 2010-04-06 | 2013-07-02 | Heliae Development, Llc | Extraction of polar lipids by a two solvent method |
WO2015140467A1 (fr) * | 2014-03-18 | 2015-09-24 | Roquette Freres | Procédé de perméabilisation thermique d'une biomasse de microalgues |
US9200236B2 (en) | 2011-11-17 | 2015-12-01 | Heliae Development, Llc | Omega 7 rich compositions and methods of isolating omega 7 fatty acids |
CN106488927A (zh) * | 2014-07-18 | 2017-03-08 | 罗盖特公司 | 用于从微藻生物质提取可溶性蛋白的方法 |
CN107208124A (zh) * | 2015-01-26 | 2017-09-26 | 罗盖特兄弟公司 | 用于分级富含蛋白质微藻的生物质的组分的方法 |
CN107217010A (zh) * | 2016-09-01 | 2017-09-29 | 刘翔 | 一种液体培养冠突散囊菌的菌液分离方法 |
EP3403662A1 (fr) * | 2017-05-02 | 2018-11-21 | National Institute of Ocean Technology | Nutriment pharmacoactif et son procédé de production à partir d'algues marines |
US10519204B2 (en) | 2014-07-18 | 2019-12-31 | Corbion Biotech, Inc. | Method for extracting soluble proteins from microalgal biomass |
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KR100983023B1 (ko) | 2010-07-07 | 2010-09-17 | 한국해양연구원 | 부등편모조류 또는 착편모조류에 속하는 미세조류의 지방산으로부터 트리글리세라이드 또는 지방산메틸에스테르를 추출하는 방법 및 이를 이용한 바이오디젤 제조방법 |
WO2012081931A2 (fr) * | 2010-12-17 | 2012-06-21 | Kim Sung-Chun | Procédé et appareil utilisables en vue de la production de cellules et de matériaux solubles dans les graisses par culture de cellules |
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2006
- 2006-02-09 WO PCT/KR2006/000462 patent/WO2006095964A1/fr active Application Filing
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Also Published As
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KR20060097947A (ko) | 2006-09-18 |
KR100657637B1 (ko) | 2006-12-19 |
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