Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
  • A01G18/00—Cultivation of mushrooms
  • A01G18/20—Culture media, e.g. compost
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H15/00—Fungi; Lichens
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor

Definitions

• the present invention relates to a Rhizopus microsporus and a method for production thereof, more particularly a method for production being produced a Rhizopus microsporus strain (Rhizopus microsporus var.
• oligosporus a mutant of Pleurotus ostreatus, Phellinus linteus, Cordyceps sp., Ganoderma LucidumCFR.
• Karst Agaricus or Rhizopus microsporus, by culturing in medium comprising at least one cereals selected from a group consisted of an unpolished rice, an unpolished glutinous rice, a bean, a polished rice and the like, and preferably a bean.
• a mushroom contains a several component effective at a prevention of geriatric diseases and the other diseases because the component is effective for increase of immunity and anticancer.
• a mushroom is generally classified with a mycelium and a fruit body.
• a mycelium has a form of thread-shape that a spore of mushroom is sprouted and plentiful physiologically active material, whereas a fruit body is a bud sprouted from a mycelium and it is called generally as a mushroom.
• the products using a mushroom are mainly marketed as a functional processed foodstuffs or pharmaceutical composition that comprise a special component, particularly a polysaccharide knowing as an effective ingredient of mushroom, extracted and purified from a mushroom, and is prepared with a form of beverage, tablet, capsule and the like.
• a health food comprising a crude mycelium of mushroom cultured at a special medium, for example the product using a mixture of mycelium of mushroom such as mushroom rice and culturing medium in oneself with a form of health food, is showed up with a form of beverage and so on.
• "Rhizopus microsporus" is an edible mycelium of mushroom growing naturally at leaves of banana.
• Rhizopus microsporus contains an element called "infraphone" inhibiting a malignant E. coli by activating an intestinal immunity.
• a Rhizopus microsporus has an effect for treatment of anemia due to abundant vitamin B group, it has been known that it also has an excellent function for geriatric diseases and prevention of aging because it has a function eliminating an activated oxygen, namely antioxydation function, and it can provide an excellent digestive function, nutrient donor, biofunctionality, mediating power and so on.
• the kinds of mushroom being cultured as a table use or a medical use are Song-i mushroom (Tricholora matsutake Sing), P'yogo mushroom (Lentimus edodes), Mogi mushroom (black fungus), Paeng-i mushroom, Phellinus linteus, Cordyceps sp., Ganoderma Lucidum(FR.) Karst, Agaricus mushroom and so on, and its cost depend on how difficult the culture is.
• Rhizopus microsporus var. chinensis Rhizopus microsporus var. microsporus
• Rhizopus chinensis Rhizopus cohnii, Mucor rhizopodiformis
• Rhizopus microsporus belong to radial fungi species taxologically and, in past generally used as agents to eliminate an odor of pig' compost and so on.
• Rhizopus microsporus mutant and its side products are disclosed at Korea patent application No. 2002-0044035 and Korea patent application No. 2001-0071879, among the above patents, Korea patent application No. 2001- 0071879 particularly disclose a novel antioxidative compounds obtained from Rhizopus microsporus by purely isolating and antioxidative composition comprising it as an effective ingredient.
• Rhizopus microsporus strain (Rhizopus microsporus var. oligosprous) according to the present invention is a novel strain as mutant of the above Rhizopus microsporus.
• a bean is typical foodstuffs in Korea, and it is known that a bean is effective at prevention of geriatric diseases because it contains abundant protein and bioactive materials.
• a bean contains a carbohydrate, a lipid other than protein as principle ingredient, and also contains various vitamins and inorganic compound such as calcium, phosphorus, iron and potassium.
• a bean is used as various kinds of food, for example a bean-curd, a soybean paste, a soy sauce, a bean sprout, a soybean milk and so on.
• Rhizopus microsporus var. oligosporus a Rhizopus microsporus strain
• cereals selected from a group consisted of an unpolished rice, an unpolished glutinous rice, a bean, a polished rice and the like, and preferably a bean as a culturing medium. Namely, there is advantage realizing the above beneficial effect in intaker' body when using the above Rhizopus microsporus as a table use.
• Rhizopus microsporus mutant Rhizopus microsporus var. oligosporus
• the above object of the present invention is achieved by examining genetics of Rhizopus microsporus mutant of the present invention and by providing a method of mass-production through searching an effectual medium and a culturing condition.
• Rhizopus microsporus The result of base analysis for the above Rhizopus microsporus is as followings:
• a method culturing and producing a mycelium of the above Rhizopus microsporus and its method is as following: ⁇ 55>
• the above method comprises following steps; ⁇ 56> pre-fermenting after swelling a peeled off and washed bean in the warm water of 43 ⁇ 47°C during 11 ⁇ 13 hours; ⁇ 57> sterilizing at high temperature of 100 ° C during 3 - 5 minutes after collecting the fermented bean and washing it with pure water; ⁇ 58> eliminating a moisture by collecting the above sterilized bean and standing it at room temperature during 50 ⁇ 70 minutes; ⁇ 59> inoculating a seed strain of Rhizopus microsporus mutant at the above bean eliminated a moisture and sprouting at the temperature of 30 ⁇ 35 ° C under the humidity of 60 ⁇ 70% during 24 ⁇ 36 hours; and ⁇ 60> harvesting a mycelium of mushroom taken root and a
• the amount of the above seed strain of Rhizopus microsporus mutant is ranged with a ratio of 0.1 ⁇ 0.5%(0.1 ⁇ 0.5g/kg).
• the above Rhizopus microsporus can be cultured by using boiled rice, boiled barley, boiled job' tears, boiled corn, boiled kaoliang or boiled cereals instead of boiled bean as the above medium.
• the ratio of a sprouted mushroom and a bean in the obtained product can be various, but preferably 50:50.
• the above mushroom can be Pleurotus ostreatus, Phellinus linteus, Cordyceps sp. , Ganoderma Lucidum(FR.) Karst or Agaricus.
• mushroom and bean or, for example cereal mushroom cultured by boiled rice, boiled barley, boiled job' tears, boiled corn, boiled kaoliang or boiled cereals as principle materials.
• the seed strain according to the present invention is a mutant of Rhizopus microsporus Rhizopus microsporus var. oligosprous, and it is deposited to Korean Collection For Type Cultures (KCTC) residence at Euen- dong 52, Yousung-ku, Deajeon-shi, Repubric of Korea on Feb. 13, 2004 with a deposit number of KCTC 10597BP to acquire easily for the public.
• KCTC Korean Collection For Type Cultures
• a bean of 8 kinds such as a soybean, a black bean, a bean having a taste of chestnut, a blue bean, a pea, a kidney bean, a brindled bean, and a Suritae are weighed with each 1kg, and these beans are peeled off and washed. And then washed beans are pre-ripen by swelling in the warm water of 45 ⁇ 47 ° C , preferably 45 ° C, during 11 ⁇ 13 hours, preferably 12 hours.
• each experimental medium using boiled barley, boiled job' tears, boiled corn, boiled kaoliang or boiled cereals can be prepared as the same with the above method.
• the above medium is transferred to a bioclean room by ultraviolet and cooled naturally. And then a seed strain of Rhizopus microsporus mutant is inoculated to the above medium with the syringe sterilized at clean room with a ratio of 0.1 - 0.5%(0.1 - 0.5g/kg), preferably 2%(0.2g/kg), and mixed homogenously, and then poured to the vessel punched at bottom with predetermined size to be sprouted at a culturing room during about 24 - 36 hours until being sprouted homogenously.
• the syringe used at inoculation must be consistently sterilized with a flame of alcohol lamp to prevent from introducing the other strains.
• the alcohol with 90% of purity is sprayed to the wall side, the ceiling and the bottom by means of a sprayer. It can be observed that a mycelium of mushroom is taken root depend on the lapse of time.
• Rhizopus microsporus is obtained by harvesting when reaching at 50% : 50% of the ratio of a mycelium of mushroom for a bean after sprouting during 24 - 36 hours from the time of inoculation.
• the Rhizopus microsporus sprouted according to the method of the present invention can be taken to a body as various foodstuffs.
• ⁇ 81> An analysis of ingredient for Rhizopus microsporus obtained at Example 1 is carried out. The result is shown at the below table 1.
• Rhizopus microsporus is a good health food containing abundant protein and various minerals and vitamins.
• Rhizopus microsporus The method for production of a Rhizopus microsporus is provided according to the present invention, wherein the seed strain of Rhizopus microsporus mutant is cultured with the medium using an edible cereals, therefore all the culture broth can be utilized as a functional food and so on.
• the Rhizopus microsporus according to the present invention is a nutrient food containing abundant protein and various minerals and vitamins, and also became well digested by having character of a bean and a mushroom simultaneously, and it can be utilized as a health food which can prevent from aging and a geriatric diseases as Super Oxide Dismutase food, as well as a diet food providing a good protein.

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• 2004
  • 2004-04-02 KR KR20040022867A patent/KR100668989B1/ko not_active IP Right Cessation
  • 2004-04-12 WO PCT/KR2004/000842 patent/WO2005094564A1/en active Application Filing

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