WO2005021016A1 - Preparation pharmaceutique traitant l'hypermenorrhee et son procede d'obtention - Google Patents

Preparation pharmaceutique traitant l'hypermenorrhee et son procede d'obtention Download PDF

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Publication number
WO2005021016A1
WO2005021016A1 PCT/CN2004/000778 CN2004000778W WO2005021016A1 WO 2005021016 A1 WO2005021016 A1 WO 2005021016A1 CN 2004000778 W CN2004000778 W CN 2004000778W WO 2005021016 A1 WO2005021016 A1 WO 2005021016A1
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weight
parts
hours
water
ethanol
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PCT/CN2004/000778
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English (en)
Chinese (zh)
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Zhihua Xu
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Shandong Newtime Pharmaceuticals Co., Ltd.
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Publication of WO2005021016A1 publication Critical patent/WO2005021016A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/739—Sanguisorba (burnet)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A61K36/744—Gardenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Definitions

  • composition for treating menorrhagia and preparation method thereof
  • the invention relates to a medicinal composition for treating menstruation and a preparation method thereof.
  • the medicinal composition is prepared from a Chinese herbal medicine as a raw material. Background technique
  • menstrual cycle remains the same, and the menstrual flow is more than normal, or the menstrual period is prolonged, and the amount is increased. It is called "menorrhagia".
  • the pathogenesis of menstruation is mainly caused by qi deficiency or blood heat. It is due to the endowment of Fu Sheng, yang is more than yang, qi is hot, and heat is increased by blood overflow; or over-exhausted, hot, impulsive, forced blood, resulting in increased blood volume.
  • An object of the present invention is to provide a new pharmaceutical composition for treating menstruation.
  • Another object of the present invention is to provide a method for preparing the pharmaceutical composition.
  • the pharmaceutical composition of the present invention is guided by the theory of traditional Chinese medicine, combined with modern medicine's recognition of menorrhagia and the latest progress in the treatment of menorrhagia by traditional Chinese medicine.
  • effective drugs are screened, and the developed menstrual menstruation is
  • the new formula has a bitter and sour taste in the formula, cold and cold, has cooling blood, purging heat, astringent to stop bleeding, and stir-fry is particularly good for stopping bleeding;
  • Eclipta officinalis is sour, cold, and enters the liver and menstrual blood.
  • good cooling blood to stop bleeding is the cure for hot blood and bleeding, the two are compatible, clearing the heat and cooling blood to stop bleeding, straight to the pathogenesis, a total of king medicine.
  • the brown charcoal tastes bitter and astringent, bitter is clearing heat, astringent and stops bleeding, and the charcoal stirs directly into the blood, and the effect of hemostasis is especially good; tanned skin is promoting blood circulation and stasis and bleeding; madder bitter cold, cooling blood and bleeding; both have blood circulation.
  • Puhuang and Pampas can be used with Puhuang and Pampas to relieve qi and stagnation, and relieve pain with blood.
  • Angelica sinensis sexual warmth can be scattered, sweetness can be slow, and body moisturizing can make up.
  • the effect of clearing heat and cooling blood to stop bleeding, regulate menstruation and stop bleeding, is consistent with the attending syndrome, etiology and pathogenesis, and it will have good results.
  • the pharmaceutical composition of the present invention is made of raw materials of Chinese medicinal materials including raw material group A and raw material group B Group A is:
  • Sophora flower 1-45 parts by weight, Platycladus orientalis leaves 1-60 parts by weight, Puhuang 45 parts by weight;
  • Ingredients Group B is-Agrimonia 0-60 parts by weight Boxing Ginseng 0-60 parts by weight Thistle 0-45 parts by weight
  • the raw material Group A is preferably-Japanese Elm 2 15 parts by weight Early Lotus 1-12 parts by weight Gardenia 1-9 parts by weight
  • Sophora flowers 1-9 parts by weight Thuja leaves 1-12 parts by weight -9 parts by weight more preferably-Japanese pelvis 5-8 parts by weight Echinacea 5-8 parts by weight Gardenia 4-6 parts by weight
  • Raw material Group B is preferably:
  • Agrimonia 1-60 parts by weight Fistula ginseng 0.5-5-60 parts by weight Thistle 1. 5-45 parts by weight brown palm charcoal 0.5-45 parts by weight Dan bark 1-60 parts by weight Madder 1 45 parts by weight Raw 1- 60 parts by weight of white peony 1-45 parts by weight of yellow peony 0.5-45 parts by weight Guanzhong 1-45 parts by weight angelica 1-60 parts by weight.
  • the leaves of Ulmus pumila, locust flower, large thistle, and Platycladus orientalis may be replaced with cooling blood hemostatic agents such as small thistle;
  • Agrimonia and brown palm charcoal may be used with one or more of the astringent hemostatic drugs such as Baihe and Xueyu char Instead; danpi, huangpi, habitat, guanzhong, boxing Ginseng and Gardeniae can be replaced by one or more of heat-clearing drugs such as black ginseng, red scallion, and comfrey.
  • the ground elm is preferably fried to charcoal
  • the gardenia is preferably fried tortoise shell
  • the locust flower is preferably to stir-fried locust flower
  • the arborvitae leaf is optimized to be stir-fried cypress leaf
  • Puhuang charcoal is preferred.
  • the preparation method of the pharmaceutical composition of the present invention is as follows: first take angelica and peony skin and soak for 4-12 times with water for 0.5 to 3 hours, distill and extract for 3 to 8 hours, and add the extracted volatile oil to 5 to 15 times ⁇ ring In a water-saturated solution made of dextrin, the mixture is stirred at 30-60 ° C for 1-3 hours for inclusion, and the volatile oil inclusion compound is obtained through conventional processing; the distilled aqueous solution and the drug residue are collected separately; Eclipta, Agrimonia, Platycladus orientalis (fried) and Rubia cordifolia were extracted by refluxing with 30% -80% ethanol 1-3 times, adding 3-10 times the amount of ethanol (w / v) each time and refluxing for 0.5-5.
  • the various clinical pharmaceutical dosage forms of the pharmaceutical composition of the present invention may be granules, capsules, tablets, injections, elixirs, suppositories, pills, syrups, mixtures, powders, lotions, films, drip pills, and the like.
  • the pharmaceutical composition of the present invention has a significant effect in treating menstruation. It can enhance coagulation and hemostasis, enhance the contraction of uterus in vitro and in vivo, and participate in the regulation of hormones and cycles. Moreover, large doses of the medicament of the present invention also have anti-inflammatory effects.
  • composition of the present invention will be further described in detail in combination with the examples and the efficacy test.
  • the pharmaceutical composition of the present invention (5g ⁇ kg— 1 10 g-kg- 1 or 20 g * kg- ') can shorten bleeding time and clotting time in mice; large doses (16 g ⁇ kg " 1 ) can significantly shorten Rat PRT time, but no significant effect on PT time and FDP amount; Observing the effect of the pharmaceutical composition of the present invention on the sex organs and sex hormones of female juvenile rats, it was found that the two dose groups (8g, kg " ⁇ 16 g-kg -) It has different degrees of elevation of FSH, LH and E 2 in rats; observation of the effect on rat uterine activity, the results show that duodenal administration (2.5 g * kg 5 g-kg- "1 and lo g * kg" can significantly increase the amplitude of uterine activity in rats; 'the pharmaceutical composition of the present invention (0.05 g, 0.1 g, 0.2 g) has a contractive amplitude and contraction frequency of three-dimensional uterine uterus in rats
  • the pharmaceutical composition of the present invention used in the following pharmacodynamic experiments is the granule of Example 32, and the dosages used in the tests refer to the amount of raw drugs; Yunnan Baiyao, Yunnan Baiyao Group Co., Ltd., batch number: 980614; oxytocin Injection, product of Shanghai Hefeng Pharmaceutical Co., Ltd., batch number: 990404, Gongxuening capsule, product of Yunnan Baiyao Group Co., Ltd., batch number: 980414; Diethylstilbestrol injection, Shanghai Ninth Pharmaceutical Factory.
  • mice Take 50 mice, half male and half female, 10 mice in each group, divided into 5 groups, namely the excipient group (equal volume), the positive control group (Yunnan Baiyao 0.6 g-kg " 1 ), the pharmaceutical composition of the present invention
  • Three dose groups (low-dose group 5 g-kg " ⁇ medium-dose group 10 g ⁇ kg" 1 , large-dose 20 g ⁇ kg- Ig, administered for 7 consecutive days, 45 min after the last dose, measured by tail trimming method Bleeding time in mice.
  • the results show that the drug of the present invention can significantly shorten the bleeding time in mice. See Table 1 for the effect on bleeding time in mice (X ⁇ S)
  • mice Take 50 mice, half male and half female, 10 mice in each group, divided into 5 groups, that is, excipient group (equal volume), positive control group (Yunnan Baiyao 0.6 g-kg " 1 ), three of the pharmaceutical composition of the present invention
  • Dose groups low-dose group 5 g-kg- medium-dose group 10 g 'kg- large-dose 20 g. Kg-.
  • Ig administration for 7 consecutive days, 45 minutes after the last administration mice were measured by capillary tube method
  • the clotting time that is, a capillary tube with an inner diameter of 1 ⁇ m was inserted into the venous plexus of the mouse's eye to take blood, and the blood was taken to 5 cm. The glass tube was broken every 30s, and the presence of clots was checked and recorded. Coagulation time. The results show that the medicament of the present invention can significantly shorten the clotting time of mice, as shown in Table 2.
  • Excipient group equal capacity 10 64.10 ⁇ 13.11
  • Drug of the invention 10 50.0 ⁇ 11.57 '
  • mice Take 50 mice, half male and half female, each group of 10, divided into 5 groups, that is, excipient group (equal volume), positive control group (Yunnan Baiyao 0.6 g-kg " 1 ), three of the pharmaceutical composition of the present invention Dose groups (low-dose group 5 g-kg " ⁇ medium-dose group 10 g, kg-, large-dose 20 g 'kg-.
  • mice half male and half female, each group of 10, divided into 4 groups, that is, excipient group (equal volume), positive control group (Yunnan Baiyao 0.6 g «kg- '), two of the pharmaceutical composition of the present invention
  • excipient group equal volume
  • positive control group Yamadao 0.6 g «kg- '
  • two of the pharmaceutical composition of the present invention Each dose group (medium dose group 10 g-kg- 1 and high dose group 20 g, kg- ')) 0 ig for 7 consecutive days, 45 minutes after the last dose, blood was drawn from the eyeballs, and RBC and WBC were routinely measured And PLT.
  • the results show that the drug of the present invention can increase RBC and WBC without significantly affecting the number of PLT, as shown in Table 5.
  • Excipient group equal volume 8.33 ⁇ 0.37 2.59 ⁇ 0.32 652.70 ⁇ 175.14 Yunnan Baiyao group 0.6 8.61 ⁇ 0.46 2.88 ⁇ 0.74 555.50 ⁇ 290.96
  • the drug of the invention 10 8.78 ⁇ 0.49 '3.35 ⁇ 0.87 "568.88 ⁇ 223.35 Group 20 8.91 ⁇ 0.46 4.87 ⁇ 0.74 '' 636.50 ⁇ 139.66
  • 'P ⁇ 0.05,' * ⁇ ⁇ 0.01
  • the uterus and ovaries were fixed with formalin, and conventional paraffin-wrapped sections were examined by HE staining. The results showed that the uterus and ovaries of the four groups of mice showed late hyperplasia or early secretion changes, and the ovaries in each group were all changed. Visible growth follicles and corpus luteum formation, but no significant difference between the groups; the drug of the present invention has no significant effect on the weight of the ovary and uterus; the drug of the present invention has different degrees of impact on FSH, LH and E 2 , It can increase its content, indicating that it is involved in the regulation of hormones, see Table 6.
  • mice Female non-pregnant SD rats were injected subcutaneously with diethylstilbestrol Img ⁇ kg two days before the experiment—once a day, twice a day. On the third day, the rats were sacrificed, a laparotomy was performed, and the uterus was found. One side of the uterus was cut out, and the uterus was carefully separated. Put the uterus in a constant temperature water bath containing 20ml De Jal on II nutrient solution, the lower end is fixed to the bottom of the water bath, and the upper end is connected to the transducer, with sufficient oxygen, 2 bubbles per second, temperature 31 ⁇ 0.
  • the drug (as described in Example 32) of the present invention is a lg crude drug ⁇ ⁇ .
  • anesthesia with 20% urethane 1 g , kg- 1 intraperitoneal injection, fixed in a supine position on a rat plate, mid-incision of the upper abdomen 1-2 cm, find and fix twelve
  • the intestine is prepared for medicinal purposes.
  • a 2cm midline incision is made in the lower abdomen to find the uterus, and the surrounding tissue is separated.
  • a section of about 3cm in length is selected at one side of the uterine horn, and a cotton thread is sewn at the midpoint to connect with the transducer.
  • the vaginal end and the ovarian end are respectively fixed on the fulcrum at both ends of the special glass tube, and the cotton thread is connected to the transducer.
  • mice Take 50 mice, half male and half male, 10 mice in each group, divided into 5 groups, namely the excipient group (equal volume), the positive control group (indomethacin 9mg. Kg " 1 ), the pharmaceutical composition of the present invention
  • Three dose groups (low-dose group 5g. Kg " 1 , medium-dose group 10g. Kg” 1 , large-dose group 20g. Kg o ig, for 7 consecutive days, 30 minutes after the last dose, on both sides of the left ear of the mouse Coated with 100% xylene, the right ear was used as a control. After 4 hours, mice were sacrificed. Two ears were cut along the baseline of the auricle, and mouse ears were prepared with a 6-diameter hole punch.
  • Indomethacin dosage unit mg. Kg-common drug 2 times.
  • the pharmaceutical composition of the present invention can enhance the coagulation and hemostatic function, and enhance the contraction effect of the uterus in vitro and in vivo. And participate in the regulation of hormones and cycles. In addition, large doses of the medicament of the invention have anti-inflammatory effects.
  • Example 1 The following are examples of preparing the pharmaceutical composition of the present invention: Example 1:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol under reflux, 5 times the amount of ethanol (v / w) was added each time, and refluxed for 2 hours each time, filtered, and set aside.
  • the above dregs and the other four flavor medicines are cooked twice, each time adding 8 times the amount of water, each cooking for 1.5 hours, filtering while hot; concentrated to a relative density of 1. 20, adding ethanol to make the alcohol content 55 %; The supernatant and the alcohol extract are combined, and concentrated under reduced pressure.
  • the concentrated liquid is added with auxiliary materials such as dextrin and sweetener. After mixing, the granules are prepared.
  • Example 2 Example 2:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol under reflux, 6 times the amount of ethanol (v / w) was added each time, and refluxed for 2 hours each time, filtered, and set aside.
  • the above medicine residue and the remaining four flavor medicines are decoated twice with water, each time adding 6 times the amount of water, each 1.5 hours, filtering while hot; concentrated to a relative density of 1. 18, adding ethanol to make the alcohol content reach 65% Supernatant and alcohol extract are combined, concentrated under reduced pressure, and water is added to the total amount of preparation, which can be made into injection by conventional techniques.
  • Example 3 Example 3:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol under reflux, 8 times the amount of ethanol (v / w) was added each time for 2 hours, filtered, and set aside. 5 ⁇
  • the above dregs and the remaining four flavor medicines were decocted twice with water, each time adding 10 times the amount of water, and decocted for 1.5 times.
  • filter it When it is hot, filter it; concentrate to a relative density of 1. 18, add ethanol to make the alcohol content reach 65%; combine the supernatant with the alcohol extract, concentrate under reduced pressure, add the specified concentration of ethanol, and make tincture by conventional techniques Just fine.
  • Example 4 Example 4:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted 3 times with 60% ethanol under reflux, each time adding 4 times the amount of ethanol (v / w), 1.5 hours each time, filtered, and set aside.
  • the above dregs and the remaining four flavor medicines were boiled 3 times with water, 12 times the amount of water each time, and simmered for 2 hours, filtered while hot; concentrated to a relative density of 1. 15, and ethanol was added to make the alcohol content reach 65%;
  • the supernatant and the alcohol extract are combined, concentrated under reduced pressure, dried to a fine powder, and added into a suitable base to make a suppository.
  • Example 5 Example 5:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol under reflux, and 3 times the amount of ethanol (v / w) was added each time, and filtered for 1 hour each time, and then used.
  • the above medicine residue and the remaining four flavor medicines were decoated twice with water for 1 hour each time and filtered while hot; concentrated to a relative density of 1.1 and ethanol was added to make the alcohol content reach 60%; the supernatant and the alcohol extract were combined It can be concentrated under reduced pressure, dried under vacuum, pulverized into fine powder, and honey pills can be made with honey as a binder.
  • Example 6 Example 6:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted 3 times with 60% ethanol under reflux, and 6 times the amount of ethanol was added each time, and each time was refluxed for 1.5 hours, filtered, and set aside.
  • the above medicine residue and the remaining four flavor medicines are decoated 3 times with water, 10 times the amount of water each time, 2 hours each time, and filtered while hot; concentrated to a relative density of 1. 15, ethanol is added to make the alcohol content reach 65%
  • the supernatant was combined with the alcohol extract, concentrated under reduced pressure, and sucrose water was added to make a concentrated sucrose aqueous solution to obtain a syrup.
  • Example 7 Example 7:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted 3 times with 60% ethanol under reflux, adding 8, 6, and 4 times the amount (v / w) of ethanol, respectively, and refluxed for 1.5 hours each time, filtered, and set aside.
  • the above medicine residue and the remaining four flavor medicines were decoated 3 times with water and added 6 times the amount of water each time, 2 hours each time, filtered while hot; concentrated to a relative density of 1.15, and added ethanol to make the alcohol content reach 65%; supernatant
  • the liquid and the alcohol extract are combined, concentrated under reduced pressure, added with dextrin, mixed with the hook, dried under vacuum, pulverized into a fine powder, and made into a powder.
  • Example 8 Example 8:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta prostrata and Platycladus orientalis leaves were extracted twice with 60% ethanol refluxing, adding 6, 4 times the amount of ethanol, respectively, refluxing for 2 and 1 hour, filtering, and setting aside.
  • the above medicine residue and the remaining four flavor medicines were decoated twice with water, adding 10, 8 times the amount of water, decocting for 2, 1.5 hours, and filtering while hot; concentrated to a relative density of 1. 15, and adding ethanol to make the alcohol content Up to 65%; the supernatant and the alcohol extract are combined, concentrated under reduced pressure, dried, added with dextrin, water and suitable film-forming materials, and processed into a film agent.
  • Example 9 Example 9:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol refluxing, adding 8, 6 times the amount (vZw) of ethanol, respectively, and refluxing for 2.5, 2 hours, filtered, and set aside.
  • the above medicine residue and the remaining four flavor medicines were decoated twice with water, and the water was added with 6 times and 4 times the amount, respectively, and each time was cooked for 1 hour, filtered while hot; concentrated to a relative density of 1.15, and ethanol was added to make the alcohol content reach 65. %; Combine the supernatant with the alcohol extract, add water to the total amount, add the appropriate amount of stevia, filter, fill, and sterilize to obtain the oral solution.
  • Example 10 Example 10:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta prostrata and Platycladus orientalis leaves were extracted with 60% ethanol under reflux for two times, and ethanol was added 5 or 3 times (v / w), and each time refluxed for 1.5 hours, filtered, and used.
  • the above medicine residue and the remaining four flavor medicines are decoated twice with water and added with 12 and 10 times the amount of water respectively, each time being cooked for 1.5 hours, filtered while hot; concentrated to a relative density of 1.15, and added ethanol to make the alcohol content reach 65%; the supernatant and the alcohol extract are combined, and water is added to the total amount configured, filtered, filled, and sterilized to obtain a lotion.
  • Example 11 Example 11:
  • the mangosteen is charcoal with chard, gardenia, locust flower, Platycladus orientalis, and charcoal.
  • Eclipta officinalis and Platycladus orientalis leaves were extracted by refluxing with 60% ethanol twice, adding 6 times the amount of ethanol (v / w) each time, refluxing for 1.5 hours each time, filtering, and using.
  • the above dregs and the remaining four flavor medicines were decocted twice with water, each time 6 times the amount of water and decocted for 1.5 hours, filtered while hot; concentrated to a relative density of 1. 15, and ethanol was added to make the alcohol content reach 65%;
  • the supernatant and the alcohol extract are combined, concentrated under reduced pressure to obtain an extract, spray-dried, and gelatin is used as a matrix to make dripping pills.
  • Embodiment 12 Embodiment 12:
  • the ground elm is fried with charcoal, gardenia, locust flower, Platycladus orientalis, and charcoal
  • Eclipta japonica and Platycladus orientalis leaves were extracted 15 times (v / w) with 60% ethanol and refluxed for extraction twice, each time 1.5 times, Filter and set aside.
  • the above medicine residue and the remaining four flavor medicines are added with 18 times the amount of water, and cooked twice, 1.5 hours each time, and filtered while hot; concentrated to a relative density of 1.1, and ethanol to make the alcohol content reach 65%; supernatant
  • the liquid and the alcohol extract are combined, concentrated under reduced pressure to obtain an extract, dried, granulated, and compressed.
  • Example 13 Example 13:
  • the ground elm is fried with charcoal, gardenia, locust flower, Platycladus orientalis, and charcoal
  • Eclipta officinalis and Platycladus orientalis leaves were extracted twice with 60% ethanol under reflux, and each time 4 times the amount of ethanol (v / W ) was added and refluxed for 1.5 hours, filtered, and set aside.
  • Sophora flower 5kg Platycladus orientalis 5kg Puhuang 5k g
  • the ground elm is charcoal, the gardenia is preferably fried gardenia, the locust flower is fried locust, the arborvitae leaf is fried cypress, and the Puhuang is the best.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is fried locust
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal
  • the gardenia is preferably fried gardenia
  • the locust flower is stir-fried locust flower
  • the arborvitae leaf is fried cypress leaf
  • the Puhuang is charcoal.
  • the ground elm is charcoal, the gardenia is preferably fried gardenia, the locust flower is fried locust flower, the arborvitae leaf is fried cypress leaf, and the Puhuang is the best.
  • the above medicine residue and the remaining eleven flavor medicines are boiled twice with water, and each time 6 times the amount of water is cooked for two hours, and filtered while hot; the filtrate is combined with the above Dan
  • the distilled aqueous solution such as peel is concentrated to a relative density of 1.16, and ethanol is added to make the alcohol content reach 40%; the supernatant is concentrated, and the volatile oil ⁇ -cyclodextrin inclusion compound is added to dry, and magnesium stearate, sweetness is added.
  • Example 23 mixing the hooks with the granules by dry method to make granules.
  • the above medicine residue and the remaining eleven flavor medicines are decoated 3 times with water, each time 6 times the amount of water and decocted for 2 hours, and filtered while hot; the filtrate is combined with the distilled aqueous solution such as tannin, and concentrated to a relative density of 1.
  • Add ethanol to make the alcohol content reach 65%; concentrate the supernatant, dry and add the volatile oil e-cyclodextrin inclusion compound, add magnesium stearate, sweetener and mix well, dry granulate to make granules.
  • Agrimony 12k , g boxing ginseng 9kg big thistle 15kg
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours, and distill for 5 hours to extract the volatile oil. Add 5 times ⁇ -cyclodextrin inclusion to the volatile oil to make a saturated solution of water. Stir for 2 hours at 40 ° C for inclusion and refrigerate for 24 hours. , Filtered and dried at 50 ° C to obtain inclusion complex. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w) each time, and refluxing 2 times each time. Hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg First take angelica and peony peel and soak in 4 times water for 3 hours, steam for 5 hours to extract the volatile oil, add 10 times ⁇ -cyclodextrin inclusion to the volatile oil to make a saturated solution of water, and stir for 2 hours at 40 'C for inclusion and refrigerate for 24 hours. After hours, filtration and drying at 50 ° C gave the inclusion compound.
  • the steamed water solution and the medicine residue were collected separately; Eclipta prostrata, Agrimonia, Platycladus orientalis, and Rubia cordifolia were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / V) each time. 2. 5 hours, filter and set aside.
  • Guanzhong 5k g white pupa 5kg First take angelica and peony peel and soak in 12 times water for 0.5 hours. Distill 5 hours to extract the volatile oil. Add 8 times ⁇ -cyclodextrin into the water-saturated solution of the volatile oil, stir at 40'C for 2 hours, and refrigerate. After 24 hours, it was filtered and dried at 50 ° C to obtain the inclusion compound. Distilled water solution and drug residue were collected separately; Eclipta, celestial grass, Platycladus orientalis, and Rubia cordifolia were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w), and refluxing 3 times each. Hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 7 times water for 2 hours. Distill 7 hours to extract the volatile oil. Add 13 times ⁇ -cyclodextrin into the water-saturated solution of the volatile oil, stir at 40 ° C for 1 hour, and refrigerate for 24 hours. , Filtered, 50. C is dried to obtain the inclusion compound. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w) each time, and refluxing 2 times each time. 5 hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours. Distill the volatile oil for 5 hours to extract the volatile oil. Add 13 times P cyclodextrin to form a water-saturated solution. Stir for 2 hours at 40 ° C for inclusion. Refrigerate for 24 hours. , Filtered and dried at 50 ° C to obtain inclusion complex. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w) each time, and refluxing 2 times each time. 5 hours, filtered, set aside.
  • 5kg white peony 5kg 0 take angelica, peony peel and 8 times water for 2 hours, distill for 5 hours to extract volatile oil, add 9 times ⁇ -cyclodextrin inclusion water-saturated solution, and stir at 40 ° C for 2 hours Inclusion, refrigerate for 24 hours, filter, and dry at 50 ° C to obtain inclusion compound. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol each time (v / W ), and refluxing 2 times each time. 5 hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours. Distill the volatile oil for 5 hours to extract the volatile oil. Add 7 times ⁇ -cyclodextrin inclusion to make the water saturated solution. Stir for 2 hours at 40 ° C for inclusion. Refrigerate for 24 hours. , Filtered and dried at 50 ° C to obtain inclusion complex. The steamed water solution and the medicine residue were collected separately; Eclipta prostrata, Agrimonia, Platycladus orientalis, and Rubia cordifolia were extracted by refluxing with 65% ethanol twice, each time adding 5 times the amount of ethanol (v / w), and refluxing each time. 2. 5 hours, filter and set aside.
  • the above medicine residue and the other eleven flavor medicines are decoated twice with water, each time 6 times the amount of water and decoiled for 2 hours, and filtered while hot; Combine the filtrate with The distilled aqueous solution such as the tannin, was concentrated to a relative density of 1.15, and ethanol was added to make the alcohol content reach 55%; the supernatant was concentrated, dried, and added with dextrin, powdered sugar, and sweetener to make granules. The granules are obtained by drying and adding volatile oil ⁇ -cyclodextrin inclusion compound.
  • Example 42 Example 42:
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours, and distill for 5 hours to extract the volatile oil. Add 5 times ⁇ -cyclodextrin inclusion to the volatile oil to make a saturated solution of water. Stir for 2 hours at 40 ° C for inclusion and refrigerate for 24 hours. , Filtered and dried at 5 ° C to obtain the clathrate compound. The distilled aqueous solution and medicinal residue were collected separately; Eclipta prostrata, Agrimonia, Platycladus orientalis, and Rubia cordifolia were extracted by refluxing with 65% ethanol twice each time. 5 times the amount (v / w), reflux for 2.5 hours each time, filter, and set aside.
  • the above medicine residue and the remaining eleven flavor medicines are decocted twice with water, and 6 times the amount of water each time and decocted for 2 hours.
  • Filtration Combine the filtrate with the distilled aqueous solution such as tannin, and concentrate to a relative density of 1.2; add ethanol to make the alcohol content reach 55%; the supernatant is concentrated, dried, and added with dextrin, powdered sugar, and sweetener Mix the hook, make granules, dry and add volatile oil ⁇ -cyclodextrin inclusion compound and mix to obtain granules.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours, and distill for 5 hours to extract the volatile oil. Add 13 times ⁇ -cyclodextrin to the volatile oil to make a water-saturated solution. Stir for 3 hours at 60 ° C for inclusion and refrigerate for 24 hours. , Filtered and dried at 50 ° C to obtain inclusion complex. Distilled aqueous solution and drug residue were collected separately; Eclipta, celestial grass, Platycladus orientalis, and Rubia cordifolia were extracted by refluxing with 65% ethanol twice, adding 3 times the amount of ethanol (v / w) each time. 5 hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours, and distill for 5 hours to extract the volatile oil. Add 15 times ⁇ -cyclodextrin into the water-saturated solution of volatile oil, and stir for 2 hours at 40 ° C for inclusion. Refrigerate for 24 hours , Filtered and dried at 50 ° C to obtain inclusion complex. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w) each time, and refluxing 2 times each time. 5 hours, filtered, set aside.
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours, and distill for 5 hours to extract the volatile oil. Add 12 times ⁇ -cyclodextrin to make the volatile oil into a water-saturated solution. Stir for 2 hours at 40 ° C for inclusion and refrigerate for 24 hours. , Filtered and dried at 50 ° C to obtain inclusion complex. Distilled aqueous solution and medicine residue were collected separately; Eclipta, Californian, Herba orientalis, and Rubiaceae were extracted by refluxing with 65% ethanol twice, adding 5 times the amount of ethanol (v / w) each time, and refluxing 20 times each time. Hours, filtered, set aside.
  • the above dregs and the remaining eleven flavor medicines are decocted twice with water, each time 6 times the amount of water and decocted for 2 hours, and filtered while hot; the filtrate is combined with the above-mentioned steamed aqueous solution such as tannin, and concentrated to a relative density of 1 18, add ethanol to make the alcohol content reach 50%; the supernatant is concentrated, dried, added dextrin, powdered sugar, sweetener and mixed to make granules, add volatile oil ⁇ cyclodextrin inclusion compound and mix well Get granules.
  • Example 46 Example 46:
  • Guanzhong 5kg Baiji 5kg Take angelica and peony peel and soak in 8 times water for 2 hours. Distill the volatile oil for 5 hours to extract the volatile oil. Add 7 times ⁇ -cyclodextrin inclusion to the volatile oil, and stir for 2 hours at 4CTC for inclusion. After refrigerated for 24 hours, filter , 50 ° C dry Dry to get clathrate. Distilled water solution and medicinal residue were collected separately; Eclipta prostrata, Agrimonia, Platycladus orientalis, and Rubia cordifolia were re-extracted with 65% ethanol twice, each time adding 5 times the amount of ethanol (v / w), and each time reflowing 2 5 hours, filtered, set aside.
  • the above medicine residue and the remaining eleven flavor medicines are decoated twice with water, each time 6 times the amount of water and decoiled for 2 hours, and filtered while hot; the filtrate is combined with the distilled aqueous solution such as the tannin, and concentrated to a relative density of 1.

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Abstract

L'invention porte sur de nouvelles préparations pharmaceutiques traitant l'hyperménorrhée et utilisant des substances de la pharmacopée chinoise dont Sanguisorba officinalis L., Eclipta prostrala L., Gardenia jasminoides Ellis, Sophora japonica L., Biota orientalis (L.) Endl., Typha angustata Bory et Chaub, Typha angustifolia L., Typha latifolia L. et autres, et recourant à leur différentes propriétés chimiques et physiques. Leur préparation se fait dans des conditions cliniquement acceptée par extraction, concentration, séchage et mise en forme. La formule magistrale de l'invention est unique et ses effets sont significatifs
PCT/CN2004/000778 2003-09-03 2004-07-09 Preparation pharmaceutique traitant l'hypermenorrhee et son procede d'obtention WO2005021016A1 (fr)

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CN100394936C (zh) * 2005-11-17 2008-06-18 西安千禾药业有限责任公司 一种治疗崩漏的药物及其制备方法
CN1814195B (zh) * 2005-12-05 2011-04-06 杨雄志 一种治疗月经病的中药组合物及其制备方法
CN102343007B (zh) * 2011-09-27 2013-12-18 济川药业集团股份有限公司 一种具有止血功能的中药组合物
CN102626163B (zh) * 2012-04-23 2013-05-01 北京绿源求证科技发展有限责任公司 一种有益调养月经的营养食品调经茶冲剂
CN103784591B (zh) * 2014-03-04 2015-11-25 张安美 产后护理中药组合物
CN105412493A (zh) * 2016-01-18 2016-03-23 黄庆辉 一种治疗血热型月经过多的中药
CN105582476A (zh) * 2016-02-25 2016-05-18 林立朋 一种用于治疗月经不调的药物制剂及其用途
CN105943774A (zh) * 2016-05-01 2016-09-21 邹士东 一种用于治疗月经过多的药物制剂及其制备方法
CN105726850A (zh) * 2016-05-02 2016-07-06 邹士东 一种治疗烧烫伤、外伤出血的药物制剂及其制备方法

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* Cited by examiner, † Cited by third party
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CN1135905A (zh) * 1995-05-18 1996-11-20 辽宁中医学院附属医院 调经药
CN1203805A (zh) * 1998-06-04 1999-01-06 李胤良 防治月经病证保健饮料

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1135905A (zh) * 1995-05-18 1996-11-20 辽宁中医学院附属医院 调经药
CN1203805A (zh) * 1998-06-04 1999-01-06 李胤良 防治月经病证保健饮料

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