WO2003073859A1 - Procedes de sterilisation, agent de lavage/sterilisation et procede de lavage - Google Patents

Procedes de sterilisation, agent de lavage/sterilisation et procede de lavage Download PDF

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Publication number
WO2003073859A1
WO2003073859A1 PCT/JP2003/001580 JP0301580W WO03073859A1 WO 2003073859 A1 WO2003073859 A1 WO 2003073859A1 JP 0301580 W JP0301580 W JP 0301580W WO 03073859 A1 WO03073859 A1 WO 03073859A1
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WIPO (PCT)
Prior art keywords
enzyme
detergent composition
concentration
lytic
disinfecting
Prior art date
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PCT/JP2003/001580
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English (en)
Japanese (ja)
Inventor
Seiichi Tobe
Hiroshi Akimoto
Original Assignee
Lion Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lion Corporation filed Critical Lion Corporation
Priority to JP2003572393A priority Critical patent/JP4645795B2/ja
Priority to KR1020047013659A priority patent/KR100966758B1/ko
Priority to AU2003212002A priority patent/AU2003212002A1/en
Publication of WO2003073859A1 publication Critical patent/WO2003073859A1/fr
Priority to HK05111377.5A priority patent/HK1079399A1/xx

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases

Definitions

  • the present invention relates to a disinfecting treatment method, a disinfecting detergent composition and a washing method.
  • the present invention relates to a disinfection treatment method, a disinfection cleaning composition, and a washing method for treating a substance to which cells have adhered using a lytic enzyme to efficiently remove bacteria.
  • a method of treating and removing bacteria with attached bacteria there are a method using a bactericide and a method using a bleaching agent (JP-A-63-131124, JP-A-2000-200). 0-268984, JP-A-63-286161, JP-A-11-27997, etc.).
  • a composition for disinfecting a contact lens comprising an enzyme acting on a cell wall of a microorganism, an enzyme acting on a cell membrane, an antibacterial protein and an antifungal agent, has been proposed as one utilizing an enzymatic reaction (Japanese Patent Application Laid-Open No. HEI 9-163572). No. 9-210288).
  • the above technology requires a germicide or a special chemical component that is highly irritating to the human body, and a method and composition for efficiently removing bacteria using components that are less irritating are desired.
  • the above-mentioned technology is intended for living cells, but most of the cells that cause odors such as sinks and bathrooms and other odors such as slimes and fibers in the surrounding water environment Since these are dead, methods and compositions that can efficiently decompose and remove such dead cells have been desired. Disclosure of the invention
  • An object of the present invention is to provide a disinfecting treatment method, a disinfecting detergent composition, and a washing method that can efficiently decompose and remove cells using components having low irritation.
  • the present inventors have disclosed that a lytic enzyme having chelating agent resistance is contained at a concentration that gives a specific lytic activity, that a calcium ion concentration is a specific amount, and that the pH of the cells is greater than 8 and 12 or less.
  • the present inventors have found that by treating the attached substance, dead cells can be efficiently decomposed and removed, and the present invention has been accomplished.
  • the present invention provides the following disinfecting method, disinfecting detergent composition and washing method for fibers. Offer.
  • (1) Contains a chelating agent-resistant lytic enzyme at a concentration at which the lytic activity becomes 2.5 UZmL or more, the calcium ion concentration is 1 Oppm or less in terms of Ca ⁇ , and the pH is more than 8 and 12 or less.
  • a method for disinfecting bacteria comprising treating a substance to which bacterial cells adhere using an aqueous solution of the microorganism.
  • a substance containing a lytic enzyme having chelating agent resistance at a concentration at which the lytic activity is 15 UZmL or more, and the substance to which the cells are adhered is treated using an aqueous solution having a pH of more than 8 and 12 or less. To remove bacteria.
  • a disinfecting detergent composition containing a lytic enzyme having chelating agent resistance and containing no phosphate, wherein the disinfecting detergent composition or a water dilution thereof has a lytic activity on the lytic enzyme.
  • a disinfecting detergent composition comprising a solution having a pH of at least 15 U / mL and a pH of from 8 to 12.
  • the fibers are sterilized and washed using an aqueous solution of a disinfecting detergent that has been adjusted to one of the following to suppress the generation of offensive odors when fibers are dried indoors or when fibers are stored after drying. How to wash fibers.
  • a disinfecting detergent that has been adjusted to one of the following to suppress the generation of offensive odors when fibers are dried indoors or when fibers are stored after drying. How to wash fibers.
  • a lytic enzyme having chelating agent resistance refers to a lytic enzyme in the presence of a chelating agent. Refers to a lytic enzyme whose lytic activity does not decrease. Specifically, the “unit
  • a protease having lytic activity is preferable.
  • the unit of lytic activity (U) is defined by the following method.
  • Dissolve the enzyme in sterile water distilled water that has been heat-sterilized at 121 ° C for 20 minutes.
  • EDTA ⁇ 4 sodium, 11 1 pm, sodium bicarbonate, 278 ppm, and dissolve the enzyme to pH 8.5 with sodium hydroxide.
  • Heated cells of Escherichia coli Escherichia coli ATCC 8739: Escherichia coli
  • OD 660 nm turbidity turbidity at a wavelength of 660 nm in a spectrophotometer
  • the lysis rate is measured by the following method.
  • Dissolve Brainheart Infusion Medium manufactured by Difco
  • a specified amount 37 gZL
  • purified water a specified amount (37 gZL) of purified water
  • 5 OmL a 50-OmL Sakaguchi flask and sterilize.
  • Collect the cells by centrifugation (3,000 rpm (approximately 1800 G) for 5 minutes), suspend the cells in sterile water, perform centrifugation again, collect the cells, suspend the cells in sterile water, and add the cells to sterile water.
  • the suspended cells are heated at 90 ° C for 20 minutes, centrifuged, and the heated cells are collected and killed. Suspended in bacterial water to obtain a bacterial suspension with a turbidity of ⁇ D 660 nm of 3.3. (Measurement of lysis rate)
  • the lysis rate is determined by the following equation. Turbidity at the beginning of the reaction turbidity after 10 minutes
  • Turbidity at start of reaction-blank turbidity Aqueous solution of the present invention (1), sterilizing detergent composition of (a) in (3) and (5) (or its water-diluted solution) solution (hereinafter referred to as 2.
  • the solution has a lysis activity of at least 2.5 U / mL, preferably 3 to 400,000 OU / mL, and more preferably 15 to 400,000 UZmL.
  • a solution of the disinfecting detergent composition (or its water-diluted solution) of (b) in (4) and (5) of the present invention hereinafter abbreviated as a solution of 15 UZmL or more).
  • a lytic enzyme at a concentration having a lytic activity of 15 UZmL or more, preferably 16 U / mL or more, particularly 20 to 400,000 OU / mL. Adjust the concentration of the solution to achieve such lytic activity.
  • the upper limit is preferably 3, OO OU / mL for a solution of 2.5 UZmL or more, and 1,000 OUZmL for a solution of lSUZmL or more.
  • the lytic activity of the solution of the present invention is obtained by converting the concentration of each enzyme in the solution to UZ mL based on the lytic activity value (U / unit weight) of the enzyme.
  • the 2.5 UZmL or more solution and the 15 UZmL or more solution are collectively referred to simply as an aqueous solution or a sterilizing detergent composition solution.
  • the lytic enzyme specifically, Savinase 12T, Savinase 16L, Esperase 4T, Evalase 8 ⁇ , Cannase 24 ⁇ , Alcalase 2 2 (No Pozyms), a P enzyme produced from Bacilluss p. P (No. 8090), and an enzyme produced from Bacilluss p. K (No. 8091).
  • preferred enzymes are sabinase 12 ⁇ , sapinase 16L, esperase 4T, eparase 8 ⁇ , kannase 24 ⁇ : alcalase 2 ⁇ , dezyme, ⁇ enzyme and the like.
  • lytic enzyme when used in combination with an enzyme having another effect, an increase in the lytic effect can be expected.
  • lipase having an excellent cleaning effect can be mentioned.
  • the measurement of lipase activity is usually carried out by measuring the amount of free fatty acid when a substrate is decomposed using lipase.
  • the lipase blended in the detergent composition of the present invention includes: "The amount of fatty acid produced from triolein after the substrate degradation test is twice that of the amount of fatty acid produced from ⁇ -hexadecyl palmitate after the substrate degradation test.
  • a lipase having the above-mentioned (molar ratio) enzyme activity is preferable, and the enzyme activity is measured by the following substrate degradation test method.
  • a titrator TS-980 manufactured by Hiranuma Sangyo
  • This electrode is used because a pH electrode is also incorporated in the titrator.
  • the amount of fatty acid produced from each substrate is defined as the amount of KOH added dropwise for 60 minutes after the addition of the lipase thus determined. From the average of 10 repeated fatty acid productions, the amount of fatty acid produced from triolein (molar ratio) to the amount of n-hexadecyl palmitate produced, ie, the enzyme activity, is calculated.
  • Examples of the lipase having the above-mentioned enzymatic activity include a lipase derived from Humikola 'Ranuginosa' described in JP-T-111-151069. Such a lipase is commercially available from NopoZyms under the trade name “Leipex”.
  • the content of the lipase is preferably 0.01 to 5% by mass, more preferably 0.1 to 2% by mass as the enzyme preparation, based on the total amount of the aqueous solution or the sterilizing detergent composition solution.
  • amylolytic enzymes it is also preferable to use amylolytic enzymes.
  • the optimum pH as shown in JP-A-2-49584 is an alkaline side or the optimum temperature as shown in JP-T-2001-521739 is 60 ° C or less, or JP-T-11-150300.
  • calcium-resistant starch-degrading enzymes and the like as disclosed in JP-A No. 3 (Kokai).
  • starch-degrading enzymes which decompose the 1,6-bonds of starch as disclosed in JP-A-3-87176, JP-A-3-87177, JP-A-3-108482 and JP-A-6-14775.
  • amylase in which a wild-type amylase derived from the genus Baci11us has been modified by a gene mutation is also preferable.
  • amylase examples include amylase: Duramyl 60T (manufactured by Nopozymes) and pullulanase (manufactured by Sigma). These can be used alone or in an appropriate combination of two or more.
  • the amylase content is preferably 0.01 to 5% by mass, more preferably 0.1 to 2% by mass, as an enzyme preparation, based on the total amount of the aqueous solution or the sterilizing detergent composition solution.
  • the sanitizing detergent composition of the present invention does not contain phosphate. However, due to enzyme preparations, etc. 0.5% by mass or less of phosphate may be brought into the composition as a pure component.
  • the calcium ion concentration is 1 Oppm or less in terms of Ca ⁇ , preferably 0.01 to 9 ppm, and particularly preferably 0.01 to 5 ppm.
  • it may exceed 1 O ppm, but is preferably 0.01 to 20 ppm, more preferably 0.01 to 15 ppm, particularly 0.01 to 10 ppm.
  • the calcium ion concentration in terms of CaO shall conform to the calcium ion concentration measurement method (absorbance method using Bonn Chrome Violet) described in Examples of JP-A-9-1318615.
  • the calcium ion concentration in the above range is determined by combining a chelating agent having high chelating ability and a general-purpose chelating agent alone or in combination of two or more as appropriate with calcium ions in an aqueous solution or a detergent composition.
  • a chelating agent having a high chelating ability include an aminocarboxylic acid derivative-type chelating agent, a polycarboxylic acid or a salt thereof.
  • Aminocarboxylic acid derivative type chelating agents include aminomethyldaricin diacetate,] 3-alanin diacetate, iminodisuccinate, hydroxyiminodisuccinate (HI DS), serine diacetate, isoserine diacetate, and aspartate diacetate. Salts (ASDA), ethylenediaminetetraacetate (EDTA), nitrile triacetate (NTA), hydroxyethylenediaminepentaacetate (HEDTA), diethylenetriaminepentaacetate (DTPA) and the like. In addition, EGTA (ethylene glycol monobis [2-aminoethyl ether] tetraacetate) and the like can also be used. These chelating agents are preferably sodium salts and potassium salts.
  • chelating agents aminomethylglycine diacetate, 3-alanine diacetate, iminodisuccinate, hydroxyiminodisuccinate (HIDS), serine diacetate, isoserine diacetate, and aspartate diacetate (ASDA) are preferred.
  • polycarboxylic acid or a salt thereof examples include polyacrylic acid, a copolymer of maleic acid and acrylic acid, and a polymer electrolyte such as polysaccharide polycarboxylate.
  • the average molecular weight of the copolymer of polyacrylic acid, maleic acid and acrylic acid is preferably from 1,000 to 1,000,000, and more preferably from 1,000 to 100,000.
  • a sodium salt and a potassium salt are preferable.
  • polysaccharide-based polycarboxylates are used in such a manner that a polysaccharide is formed into a carboxylic acid group by an oxidizing agent (eg, hypochlorite, periodate, chlorite).
  • an oxidizing agent eg, hypochlorite, periodate, chlorite.
  • Oxidatively cleaved, polysaccharides include starch, dextrin, cellulose, hemicellulose, amylose, pectin and the like.
  • the content of oxidized anhydroglucose units is preferably 50 to 100 mol%, particularly preferably about 70 to 95 mol%.
  • oxidized starch obtained by oxidizing and opening starch is particularly preferred.
  • General-purpose chelating agents include crystalline aluminosilicates (A-type, P-type, X-type zeolite), aluminosilicates such as amorphous aluminosilicates, and crystalline alkali metal silicates such as layered silicates.
  • Low-molecular-weight oxycarboxylic acid-type chelating agents such as citric acid or a salt thereof, dalconic acid or a salt thereof, glycolic acid or a salt thereof, malic acid or a salt thereof, 1-hydroxyethane-1,1,1-diphosphonic acid (HEDP), phosphonic acid type chelating agents of aminotrimethylene phosphonic acid (ATMP), inorganic phosphates such as tripolyphosphate and pyrophosphate can be used.
  • a phosphorus-free chelating agent is particularly preferred.
  • the pH of the aqueous solution and the disinfecting detergent composition solution is 2.More than 8 in the case of SUZmL or more, and is 12 or less, preferably 8.1 to 11.In the case of 15 U / mL or more solution, the pH is more than 8 and 12 or less. , Preferably 8.1 to 11, especially 9 to 11.
  • the pH is in the above range, the activity of the lytic enzyme is increased, and the lytic effect is improved as the pH is increased.
  • the disinfecting detergent composition of the present invention can be used as a detergent for clothes, a detergent for hand-washing dishes, a detergent for baths, a detergent for washing machines, a detergent for toilets and water pipes.
  • the content of the bacteriolytic enzyme in the disinfecting detergent composition is preferably from 1,000 to 400, OO OUZg, particularly from 4,500 to 60,00 OU / g, from 30,000 to 60,000 OU / g. , OO OUZg is preferred. It is preferable that the pH of the solution is 8.1 to 11, particularly 8.5 to 11.
  • the content of lytic enzymes in the disinfecting detergent composition is 100 to 4.
  • the pH of the aqueous solution or the disinfecting detergent composition solution is preferably 8.1 to 10, particularly preferably 8.1 to 9.
  • the content of the lytic enzyme in the sterilizing detergent composition is preferably from 1,000 to 400,00 OU / g, and particularly preferably from 2,000 to 40,000 OUZg.
  • the pH of the aqueous solution or the disinfecting detergent composition solution is preferably from 8.1 to 10, especially from 8.1 to 9.
  • the content of the lytic enzyme in the disinfecting detergent composition is from 1,000 to
  • the pH of the aqueous solution or the disinfecting detergent composition solution is preferably from 8.1 to 12, particularly preferably from 8.5 to 11.
  • the content of the lytic enzyme in the disinfecting detergent composition is preferably from 1,000 to 100,00 OU / g, particularly preferably from 2,000 to 20,000 U / g. preferable. It is preferable that the pH of the aqueous solution or the disinfecting detergent composition solution is 9 to 12, particularly 9 to 11.
  • the disinfecting detergent composition of the present invention contains a surfactant in order to effectively penetrate the chelating agent into the soil and efficiently lyse the cells present therein. .
  • surfactant examples include various surfactants such as anionic surfactants, nonionic surfactants, cationic surfactants, and amphoteric surfactants.One of these may be used alone, or two or more thereof may be used. They can be used in appropriate combinations.
  • the content of these surfactants is preferably from 3 to 50% by mass, more preferably from 5 to 40% by mass, based on the total amount of the sterilizing detergent composition. Further, it is preferable that the anionic surfactant or the nonionic surfactant is the main surfactant (the surfactant having the highest content among the surfactants in the sterilizing detergent composition).
  • anionic surfactants include straight-chain alkylbenzene sulfonates having 10 to 14 carbon atoms, and lower alkyl monosulfo fatty acids having 13 to 19 carbon atoms (1 to 3 carbon atoms).
  • alkali metal salts such as sodium salts and potassium salts, amine salts, ammonium salts and the like can be used.
  • the content of the anionic surfactant is preferably 5 to 45% by mass, more preferably 10 to 35% by mass, based on the total amount of the sterilizing detergent composition.
  • Preferred nonionic surfactants include the following.
  • An EO-added nonionic surfactant (alkyl ether ethoxylate) obtained by adding an average of 5 to 30 moles of ethylene oxide (EO) to an alcohol having an average of 10 to 20 carbon atoms.
  • An EO-P ⁇ -added nonionic surfactant obtained by adding an average of 5 to 30 moles of ethylene oxide (EO) and propylene oxide (PO) to an alcohol having an average carbon number of 10 to 20.
  • a fatty acid ester type nonionic surfactant represented by the following formula (1).
  • R 1 represents an alkyl or alkenyl group having 5 to 21 carbon atoms, preferably 9 to 17 carbon atoms
  • OR 2 represents an additional unit of an alkylene oxide having 2 to 4 carbon atoms
  • EO represents singly or EO
  • n represents an average addition number of moles of OR 2, 5 to 30, preferably is a number of 5 to 20.
  • R 3 is from 1 to 4 carbon atoms, good Mashiku Represents an alkyl group of 1 to 2.
  • the content of the nonionic surfactant is preferably from 1 to 40% by mass, more preferably from 1 to 30% by mass, based on the total amount of the sterilizing detergent composition.
  • Preferred amphoteric surfactants include bayones such as amide propyl laurate laurate and amide ethyl stearate vein, and N-coconut fatty acid acyl-N-one-potoxyshethyl-N-hydroxyethylethylenedia. And imidazoline derivatives such as min sodium.
  • the disinfecting detergent composition is a detergent for clothing, a dishwashing detergent, a bath detergent, or a washing machine detergent
  • a surfactant particularly an anion surfactant, should be contained. Is preferred.
  • anionic surfactants include linear alkylbenzene sulfonates, ⁇ -sulfofatty acid alkyl ester salts, olefin sulfonates, alkane sulfonates, alkyl sulfates, and fatty acid salts.
  • particularly preferred are monosulfo fatty acid alkyl ester salts, which exhibit excellent detergency with a small amount of compounding.
  • anionic surfactants include polyoxyethylene alkyl or alkenyl sulfate, linear alkyl benzene sulfonate, ⁇ -sulfofatty acid alkyl ester salt, olefin sulfonate, and alkanesulfonic acid. Salts, alkyl sulfates, fatty acid salts and the like are preferred.
  • the concentration of the surfactant in the aqueous solution or the disinfecting detergent composition solution is preferably 1 to 20% by mass in the case of detergents for dishes and sinks, and in the case of detergents for toilets, water pipes and baths (for bathtubs). , 0.5 to 1.5% by mass, preferably 0.5 to 0.5% by mass in the case of a washing machine detergent, and 100 to 1200 ⁇ m in the case of a clothing detergent.
  • the disinfecting detergent composition of the present invention is used as a detergent for clothing
  • the disinfecting detergent composition is used as it is or as a water diluent
  • the dilution is preferably performed in a water diluent as the concentration of the disinfecting detergent composition.
  • Sterilization A suitable use concentration can be set according to the use application of the detergent composition.
  • the concentration of the dishwashing detergent used is preferably 1 to 50% by mass for washing in a sink or a washing tub, and 5 to 50% by mass for washing with a sponge, as the concentration of the disinfecting detergent composition (the same applies hereinafter).
  • the concentration used in the case of a bath detergent is preferably in the range of 1,000 to 100, O O Op pm.
  • the concentration used in the case of a washing machine detergent is preferably in the range of 1,000 to 10, OO ppm.
  • the concentration of detergent used for toilets and water pipes is preferably 3 to 100% by mass, particularly preferably 5 to 50% by mass.
  • the sanitizing detergent composition of the present invention is incorporated into dishwashing detergents, bathtub detergents, sinks, drainage detergents, toilet detergents, clothing detergents, etc., so long as the object of the present invention is not impaired.
  • Various components can be blended.
  • the disinfecting detergent composition of the present invention can be prepared in various dosage forms such as liquid, gel, spray, powder, evening bullet, briguet detergent, sheet and bar.
  • the method for preparing the disinfecting detergent composition of the present invention is not particularly limited, and it can be produced according to a conventional method for each of the above-mentioned preparations.
  • the disinfecting detergent composition of the present invention is particularly preferably used as a detergent for clothes.
  • the disinfecting and washing is performed on fibers, and the offensive odor is obtained when the fibers are dried indoors or stored after drying. It can be particularly used as a disinfecting and cleaning composition for suppressing the generation of water, that is, for indoor drying. It is also preferable for disassembling and cleaning dead sterilized bodies.
  • the method for removing bacteria according to the present invention is capable of efficiently decomposing and removing dead cells, and is more effective than the washing method of the present invention when fibers are dried indoors or when fibers are stored after drying. Generation of offensive odor can be suppressed.
  • the substance to which bacterial cells adhere is defined as a sink, a triangular corner (a container for garbage), a washing tub, a water pipe, a bathtub, a bathroom, a hard surface such as a plumber, a toilet, etc., clothing,
  • a sink a triangular corner
  • a washing tub a water pipe
  • a bathtub a bathtub
  • a bathroom a hard surface
  • a plumber, a toilet etc.
  • clothing examples include textile products such as bedding, curtains, carpets, floor mats, and cleaning tools.
  • the method of removing bacteria is not particularly limited, but a preferred method of treating with a substance to which bacterial cells adhere is shown below.
  • a treatment such as immersing the substance with the bacterial cells in the aqueous solution or applying, spraying, or flowing the aqueous solution on the substance with the bacterial cells.
  • a treatment such as immersing the substance with the bacterial cells in the aqueous solution or applying, spraying, or flowing the aqueous solution on the substance with the bacterial cells.
  • the substance to which the cells are attached is textiles such as clothing, it is preferable to wash in a washing machine or wash after soaking.
  • the lysing effect is determined by adjusting the temperature of the aqueous solution, the treatment time, the amount of the aqueous solution, and the like in the following suitable ranges. It can be further enhanced by adjusting to.
  • the temperature is preferably 10 to 90 ° C, particularly preferably 10 to 60 ° C.
  • the treatment time is preferably from 5 seconds to 10 hours, particularly preferably from 10 minutes to 60 minutes.
  • the treatment time is preferably 5 seconds to 60 minutes with an aqueous solution of 10 to 90, and particularly preferably 5 seconds to 30 minutes at 10 to 60 ° C.
  • the amount of the aqueous solution is preferably 0.01 to 0.5 mL, more preferably 0.1 to 0.3 mL, per 1 cm 2 of the surface.
  • the amount of the aqueous solution is preferably 0.01 to 0.5 mL, more preferably 0.1 to 0.3 mL, per 1 cm 2 of the surface.
  • 1-3 OmL per g of fiber is preferred.
  • the present invention it is possible to provide a disinfecting treatment method, a disinfecting detergent composition, and a washing method capable of efficiently decomposing and removing cells using components having low irritation.
  • a disinfecting treatment method a disinfecting detergent composition
  • a washing method capable of efficiently decomposing and removing cells using components having low irritation.
  • Aqueous solutions having the compositions shown in Tables 1 and 2 were prepared using tap water adjusted to 3.3 ° DH (33 mg ZL as Ca ⁇ ) with calcium chloride dihydrate.
  • the lysis rate of the obtained aqueous solution was measured based on the following measurement method.
  • the results are also shown in Tables 1 and 2.
  • the method for measuring the lytic activity of the enzyme used is shown below.
  • the enzyme activity in the table was calculated by converting the concentration of each enzyme in the solution to UZmL based on the lytic activity value (UZ unit weight) of the enzyme sample.
  • Thermolysin an enzyme having no chelating agent resistance, was used after dissolving to 10 mg / L (* in Table 2).
  • the lysis rate was measured by the following method.
  • a Brain Heart Infusion Medium (manufactured by Difco) was dissolved in a specified amount (37 g / L;) of purified water, and 5 OmL was placed in a 50-OmL Sakaguchi flask for sterilization. Thereafter, one platinum loop of Escherichia coli (Escherichia coli ATCC 8739: Escherichia coli) was inoculated and cultured with shaking at 37 ° C for 18 hours. The cells were collected by centrifugation (3, OOO rpm (about 1800 G) for 5 minutes), suspended in 30 mL of sterile water, and centrifuged again (3,000 rpm (about 1800 G)) to remove the cells.
  • Escherichia coli ATCC 8739 Escherichia coli
  • turbidity at OD 660 nm exceeds 0.7 before adding the cell suspension to the sample for which the lysis rate is to be measured, perform filtration with a Millipore filter (0.45 micron) in advance. To 9 mL of the solution, 1 mL of the cell suspension was added. In addition, it adjusted so that the above "1 OmL total lysis rate test solution" might be the concentration of the aqueous solution to be measured / the germicidal detergent composition solution. The lysis rate was determined by the following equation. Turbidity at the start of the reaction-Turbidity after 10 minutes
  • Lysis rate (%) X100 0 Turbidity at the start of reaction-blank turbidity
  • a disinfectant detergent composition having the composition shown in Tables 3 to 7, and use tap water adjusted to 3.3 ° DH (33 mg / L as CaO) using calcium chloride dihydrate.
  • the lysis rate of the aqueous solutions diluted to the working concentrations in the table was measured in the same manner as described above.
  • Tables 3 to 7 show the results.
  • the compositions in Tables 3 to 7 are the following cleaning agents, respectively.
  • the lytic activity (UZmL) in the table is based on the lytic activity value of the enzyme (UZ unit weight), and the concentration in the solution is converted to U / mL from the concentration used in the table.
  • the ion concentration and lysis rate were measured by adjusting the ⁇ test solution and lysis rate test solution '' described in the ⁇ Calcium ion concentration measurement method and lysis rate measurement method '' above to the concentrations used in the table. Things. The same applies to the following tables.
  • Table 8 shows the evaluation results for the aqueous solutions diluted with tap water at 3 ° DH to the working concentrations shown in the table, together with the results of the lysis rate.
  • the dried polo shirt and evening oor were allowed to stand in a constant temperature room at a temperature of 30 ° C and a humidity of 80% RH for 5 days, and the odor of the polo shirt and towel was evaluated for odor by a skilled monitor according to the following criteria. 5 people average).
  • Clothing detergents having the compositions shown in Tables 9 and 10 were prepared, washed and dried by the following evaluation method-2, and then evaluated for odor.
  • evaluation method-2 For the aqueous solution diluted with tap water at 3 ° DH to the working concentration shown in the table, the evaluation results are shown in Tables 9 and 10 along with the lysis rate.
  • a detergent for clothing having the composition shown in Table 11 was prepared, washed and dried by the following evaluation method-3, and then the odor was evaluated. The results are shown in Table 11 together with the lysis rate as described above.
  • Tables 1 to 11 are as follows.
  • the blending amounts in the table are values converted to pure components (excluding Na carbonate and bleach activator).
  • ⁇ -SF-Na Sodium salt of monosulfo fatty acid methyl ester having an alkyl group with 14 to 16 carbon atoms, manufactured by Lion Corporation, 70% pure, 72-73% solids
  • LAS-K Linear alkyl (C14-14) Potassium benzenesulfonate (When manufacturing detergent composition, neutralize Libon LH-200 (LAS-H pure content 96%), manufactured by Lion Corporation, with 48% potassium hydroxide aqueous solution Prepared by
  • LAS-Na Linear alkyl (10-14 carbon atoms) sodium benzenesulfonate (Lion Co., Ltd., Libon LH-200 (LAS-H pure content 96%) is neutralized with 48% sodium hydroxide aqueous solution Prepared by
  • AOS-Na Sodium ⁇ -olefin sulfonate having an alkyl group of 14 to 18 carbon atoms (Lion Corporation, 55% pure aqueous slurry)
  • AES-Na polyoxyethylene (E03 mol) sodium lauryl ether sulfate, 70% pure, manufactured by Lion Corporation
  • Cumenesulfonic acid manufactured by Albright & Wilson
  • Alkylamidopropyl acetate betaine On the other hand, manufactured by YAS
  • BRE 15 straight-chain primary alcohol ethoxylate having 12 carbon chains (average number of moles of ethylene oxide added: 15) manufactured by Lion Corporation
  • AE t 1 An average of 15 moles of ethylene oxide of Diador 13 (Mitsubishi Chemical) (Lion Chemical)
  • AE s average of 15 moles of ethylene oxide of fatty acid methyl ester having 12 to 18 carbon atoms
  • Additive (Lion Chemical) AEP Adduct of diadol 13 (Mitsubishi Chemical) with an average of 15 mol of ethylene oxide and propylene oxide with an average of 3 mol (Lion Chemical)
  • APG alkyl polydarcoside (manufactured by Cognis Japan)
  • AX Lauryl dimethylamine oxide (manufactured by Lion Corporation)
  • P enzyme Produced from Bacillus ssp.P (Principle No. 8090) and prepared by culture purification described in JP-A-8-165493.
  • K enzyme Produced from Bacillus sp. K (No. 8091) and prepared by culture purification described in JP-A-8-165493.
  • Y enzyme Produced from Bacillus ssp. Y (No. 8088, published by Bakken) and prepared by culture purification described in JP-A-8-165494.
  • Amylase 10: 1 (weight ratio) mixture of Duramyl 60 ⁇ (Nopozymes) and pullulanase (Sigma)
  • Cellulase Cellzym 0.7 ⁇ (made by Nopozymes)
  • Lipase Leipex 50 ⁇ (made by Nopozymes)
  • HIDS Sodium hydroxyiminodisuccinate (Nippon Shokubai Co., Ltd.)
  • EDTA ⁇ 4Na Sodium ethylenediaminetetraacetate
  • Phenonantophosphorus 1,10-Fuenantophorin (Nippon Kayaku Co., Ltd.)
  • Sodium tripolyphosphate Nippon Chemical Co., Ltd.
  • Cuenoic acid and its sodium salt manufactured by Fuso Chemical Industry Co., Ltd.
  • Zeolite A type zeolite (Mizusawa Chemical, Shilton B)
  • AA / MA copolymer salt sodium salt of acrylic acid / maleic acid copolymer, trade name Sokalan CP7 (BASF)
  • Na polyacrylate sodium polyacrylate (Nippon Junyaku, Aronbis S) Malic acid: manufactured by Fuso Chemical Industry Co., Ltd.
  • Carbonate Na sodium carbonate (made by Asahi Glass, grain ash)
  • Carbonic acid Carbonated room (made by Asahi Glass)
  • Na sulfate neutral anhydrous sodium sulfate (Nippon Chemical Co., Ltd.)
  • Na sulfite anhydrous sodium sulfite (manufactured by Mitsui Chemicals, Inc.)
  • Fluorescent agent CBS Tinopearl CBS—X (Ciba 'Specialty' Chemicals)
  • Fluorescent agent AMS Chinopearl AMS-GX (Chipa Specialty Chemicals)
  • Percarbonate Na coated sodium percarbonate (Mitsubishi Gas Chemical, SPC-D)
  • Bleaching activator OBC Hosokawa Micron Extrude Omics EM — Type 6, 4-decanoyloxy as a bleach activator
  • Benzoic acid and polyethylene glycol (PEG) # 6000 Lion Co., Ltd.
  • C14 alpha sodium olefin sulfonate powder Liporan PB-800, Lion Co., Ltd.
  • the mixture was charged, kneaded and extruded to obtain a noodle-shaped extruded product having a diameter of 0.8 ⁇ .
  • the extruded product (6 Ot) is kneaded and pressed by Hosokawa Micron Fitzmill DKA-3 type.
  • the bleaching activator with an average particle size of 700 im obtained by pulverization is supplied in the same direction as the raw granulated product, and 5% by mass of A-type zeolite powder is supplied as an auxiliary agent. object.
  • Fatty acid amidopropyl betaine (manufactured by Lion Corporation)
  • Alkylphenoxyphenyldisulfonic acid Na (Nikko Chemical Co., Ltd.) Stearyl trimethylammonium chloride: (Lionaxo Co., Ltd.) Didecylmethylammonium chloride (Lionaxo Co., Ltd.)
  • Synthetic polymer methacrylic acid copolymer (manufactured by YAS)
  • Triethanolamine (Nippon Shokubai Co., Ltd.)
  • Silica Silicon graft polymer (manufactured by YAS)
  • NRE2 (Lion Corporation)
  • the lytic enzyme with chelating agent resistance was contained at a concentration at which the lytic activity became 2.5 UZmL or more, and the calcium ion concentration was 10 ppm or less in terms of Ca ⁇ . It can be seen that an aqueous solution having a ratio of more than 8 to 12 or less exhibits an excellent lysis rate. In addition, a lytic enzyme with chelating agent resistance is used.
  • Nonionic surfactant (BRE15) 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 Sodium lauryl sulfate
  • Nonionic surfactant (BRE15) 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 250 Sodium lauryl sulfate 250
  • Nonionic surfactant (BRE15) 5 5 5 5 5 20 Dicyandiamide 20 20 20 20
  • Fragrance composition A B C D A Fragrance content 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
  • Fragrance composition A B D Fragrance content 0.1 0.1 0.1 0.1
  • Y enzyme 4500 Total (%) 100 100 100 100 100 100 Working concentration ⁇ ) 4000 4000 4000 4000 4000 4000 Calcium ion concentration (CaO ppm) 6 2 0.02 3 0.02 Aqueous solution pH 8.5 8.5 8.5 8.5 8.5 8.5 8.5 Lysis activity (U / niL) 18 18 18 18 0 Lysis rate (%) 55 55 55 55 2 Polo shirt odor 2 2 1 2 5 Towel odor 1 2 2 1 5 [Table 9]
  • Fragrance composition C D B A Fragrance content 0.2 0.1 0.1 0.2
  • Dezyme 2000 2000 26000 26000 Total (%) 100 100 100 100 100 Working concentration ⁇ ) 5000 5000 5000 5000 5000 5000 5000 5000 5000 5000 Aqueous solution pH 10.5 10.5 10.5 10.5 10.5 Lysis activity (U / mL) 10 10 130 130 0 Lysis rate (%) 65 65 75 75 8 Towel odor 2 2 1 1 4

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Abstract

L'invention concerne un procédé de stérilisation consistant à traiter une matière, sur laquelle sont collées des cellules bactériennes, à l'aide d'une solution aqueuse qui contient une lysokinase résistante aux chélateurs, à une concentration permettant de développer une activité lytique égale ou supérieure à 2,5U/mL, et qui présente une concentration d'ions calcium (en terme de CaO) inférieure ou égale à 10ppm, et un pH supérieur à 8 mais égal ou inférieur à 12. La présente invention concerne également un procédé de stérilisation consistant à traiter une matière, sur laquelle sont collées des cellules bactériennes, à l'aide d'une solution aqueuse qui contient une lysokinase résistante aux chélateurs, à une concentration permettant de développer une activité lytique égale ou supérieure à 15U/mL, et qui présente un pH supérieur à 8 mais égal ou inférieur à 12
PCT/JP2003/001580 2002-03-01 2003-02-14 Procedes de sterilisation, agent de lavage/sterilisation et procede de lavage WO2003073859A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003572393A JP4645795B2 (ja) 2002-03-01 2003-02-14 除菌洗浄剤組成物及び悪臭発生抑制方法
KR1020047013659A KR100966758B1 (ko) 2002-03-01 2003-02-14 제균처리 방법, 제균세정제 조성물 및 세탁 방법
AU2003212002A AU2003212002A1 (en) 2002-03-01 2003-02-14 Sterilization methods, sterilizing/washing agent and washing method
HK05111377.5A HK1079399A1 (en) 2002-03-01 2005-12-12 Sterilization methods, sterilizing/washing agent and washing method

Applications Claiming Priority (2)

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JP2002-55584 2002-03-01
JP2002055584 2002-03-01

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CN105106993A (zh) * 2015-09-02 2015-12-02 杭州满速电子商务有限公司 一种服装成品的处理方法
CN107988728B (zh) * 2017-12-27 2019-11-05 宁波高新区盛文途纺织品有限公司 一种纺织品清理方法
CN112585251B (zh) * 2018-08-23 2022-08-09 昭和工业株式会社 排水装置用清洗剂

Citations (9)

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JPS4948825A (fr) * 1972-09-13 1974-05-11
EP0204342A2 (fr) * 1985-06-06 1986-12-10 Lion Corporation Protéases alcalines, micro-organismes les produisant et détergents
JPS63234096A (ja) * 1987-03-23 1988-09-29 ピジヨン株式会社 おむつ用洗剤組成物
WO1994024240A1 (fr) * 1993-04-08 1994-10-27 The Procter & Gamble Company Tensioactifs de sulfates secondaires d'alkyle (2,3) se trouvant dans des compositions detergentes stables contenant des enzymes
JPH0892021A (ja) * 1994-09-26 1996-04-09 Kao Corp 農薬用効力増強剤組成物及び農薬組成物
JPH0910288A (ja) * 1995-06-29 1997-01-14 San Contact Lens:Kk 含水性ソフトコンタクトレンズの洗浄および消毒用組成物
JPH10195483A (ja) * 1996-12-28 1998-07-28 Lion Corp 自動食器洗浄機用洗剤
JPH11505565A (ja) * 1995-05-19 1999-05-21 ベッツディアボーン・インコーポレイテッド スライム制御剤としてのマンナナーゼの使用
JP2001064695A (ja) * 1999-08-31 2001-03-13 Lion Corp 洗浄剤組成物

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JPS61280278A (ja) * 1985-06-06 1986-12-10 Lion Corp アルカリプロテア−ゼ
JPH01111986A (ja) * 1987-10-23 1989-04-28 Sanki Seikosho:Kk 穿孔方法および装置
JP2700465B2 (ja) * 1988-05-09 1998-01-21 関西酵素株式会社 塩化リゾチーム配合トイレタリー製品
JP2676453B2 (ja) * 1992-07-02 1997-11-17 花王株式会社 アルカリイソアミラーゼ及びそれを生産する微生物並びに該アルカリイソアミラーゼの製造方法
JPH09318615A (ja) * 1996-05-31 1997-12-12 Lion Corp 水溶液中の金属イオンの定量方法
JP4382953B2 (ja) * 2000-03-16 2009-12-16 花王株式会社 ポリガラクツロナーゼ

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4948825A (fr) * 1972-09-13 1974-05-11
EP0204342A2 (fr) * 1985-06-06 1986-12-10 Lion Corporation Protéases alcalines, micro-organismes les produisant et détergents
JPS63234096A (ja) * 1987-03-23 1988-09-29 ピジヨン株式会社 おむつ用洗剤組成物
WO1994024240A1 (fr) * 1993-04-08 1994-10-27 The Procter & Gamble Company Tensioactifs de sulfates secondaires d'alkyle (2,3) se trouvant dans des compositions detergentes stables contenant des enzymes
JPH0892021A (ja) * 1994-09-26 1996-04-09 Kao Corp 農薬用効力増強剤組成物及び農薬組成物
JPH11505565A (ja) * 1995-05-19 1999-05-21 ベッツディアボーン・インコーポレイテッド スライム制御剤としてのマンナナーゼの使用
JPH0910288A (ja) * 1995-06-29 1997-01-14 San Contact Lens:Kk 含水性ソフトコンタクトレンズの洗浄および消毒用組成物
JPH10195483A (ja) * 1996-12-28 1998-07-28 Lion Corp 自動食器洗浄機用洗剤
JP2001064695A (ja) * 1999-08-31 2001-03-13 Lion Corp 洗浄剤組成物

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CN100389659C (zh) 2008-05-28
HK1079399A1 (en) 2006-04-07
KR20040089693A (ko) 2004-10-21
JPWO2003073859A1 (ja) 2005-06-23
JP4645795B2 (ja) 2011-03-09
AU2003212002A1 (en) 2003-09-16
CN1642431A (zh) 2005-07-20

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