WO2003046157A1 - Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents - Google Patents
Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents Download PDFInfo
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- WO2003046157A1 WO2003046157A1 PCT/JP2002/012319 JP0212319W WO03046157A1 WO 2003046157 A1 WO2003046157 A1 WO 2003046157A1 JP 0212319 W JP0212319 W JP 0212319W WO 03046157 A1 WO03046157 A1 WO 03046157A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Definitions
- the present invention relates to a Bacillus sp. D 747 strain and its use, and more particularly, to a plant containing the Bacillus sp. D 747 strain as an effective bacterium having an inhibitory effect on plant disease and an effect of controlling insect pests.
- the present invention relates to a disease control agent and a nox or pest control agent, and a control method using these control agents.
- Methods for controlling plant diseases and pests include cultivation or physical control using crop rotation or solar heat, chemical control using chemical pesticides, control using disease resistant varieties, and antagonistic microorganisms against attenuated viruses and pathogens.
- the biological control used and the like can be mentioned.
- chemical pesticides especially organic synthetic fungicides
- a large number of drugs having high potency and various actions have been developed one after another.
- Chemical control methods using these have greatly contributed to the control of plant diseases and labor saving in control work, and are widely used.
- the phenomenon of the so-called drug-resistant bacteria that reduces the control effect by chemical control methods has been observed in some crops and diseases, and has become a problem.
- An object of the present invention is to isolate a novel strain having an effect of suppressing the onset of a plurality of plant diseases and controlling pests or pests.
- a further object of the present invention is to provide a plant disease control agent and a Z or pest control agent which contain the above strain as an effective bacterium and can be effectively used as a biological pesticide.
- a further object of the present invention is to provide a method for controlling plant diseases and / or pests using the above-mentioned plant disease control agent and / or pest control agent.
- the present inventors have found that a novel strain belonging to the genus Bacillus isolated from the natural world has an effect of suppressing the onset of several plant diseases and an effect of controlling insect pests without affecting the growth of plants.
- the present invention has been completed.
- the present invention provides the following strains, a plant disease controlling agent and / or a pest controlling agent, and a plant disease controlling and / or pest controlling method.
- a plant disease control agent comprising a Bacillus) sp. D747 strain as an effective bacterium.
- a pest control agent comprising a Bacillus sp. D747 strain as an effective bacterium.
- the Bacillus sp. D747 strain of the present invention is a strain isolated from the air in Kikugawa-cho, Ogasa-gun, Shizuoka Prefecture. Based on the bacteriological properties described below, these strains were identified as new strains belonging to the genus Bacillus according to the Bergeys Manual of Systematic Bacteriology, volume 1 (1984). It seems to belong to B. cereus, and on January 28, 2000, “Bacillus cereus D747” was named “Bacillus cereus D747” by the Agency of Industrial Science and Technology. science and iechnology, National Institute of Bioscience and Human-Technology).
- strain D 747 Bacillus sp. Strain D 747 of the present invention (hereinafter, simply referred to as“ strain D 747 ”) was The National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary (National Institute of Advanced Industrial Science and Technology) has been deposited as Bacillus sp. D747 (F ERM P-1 8 1 2 8). 2002 (Heisei 14) 1 Transferred to the International Depositary on January 8, and at the Patent Organism Depositary Center as "Baci] .lus sp. D747J (F ERM BP—82 34) Has been deposited.
- the bacteriological properties of the D747 strain of the present invention are as follows.
- a test of mycological illness was carried out in Bergeys Manual of Systematic Bacteriology.
- Gravy agar plate culture forms white to cream-colored colonies, wrinkles on the surface
- any type can be appropriately selected, such as the type of culture medium and the culture conditions.
- a general medium such as a meat extract medium
- a medium containing glucose, peptone, and yeast extract may be used.
- a solid medium such as a slope medium containing agar and a plate medium may be used.
- the desired strain can be obtained by growing the D747 strain by culturing.
- the medium carbon source the c specific anything above strain assimilable is available Gunorekosu, galactose, Lac bets - scan, sucrose, Manore toast, malt extract, sugars such as starch hydrolysates Besides, there are various synthetic or natural carbon sources that can be used by the strain D747.
- various synthetic or natural products that can be used by the strain including organic nitrogen-containing substances such as peptone, meat extract, and yeast extract, can also be used.
- organic nitrogen-containing substances such as peptone, meat extract, and yeast extract
- Inorganic salts such as salt and phosphate, salts of metals such as calcium, magnesium, and iron, and trace nutrients such as vitamins and amino acids can also be added as needed.
- the culture can be performed under aerobic conditions such as shaking culture and aeration culture.
- the culture temperature is 20 to 30 ° C., preferably 25 to 30 ° C.
- the pH is 5 to 8, preferably 6 to 7, and the culture period is 1 to 4 days, preferably 2 to 3 The day is appropriate.
- the Bacillus sp. D747 strain of the present invention may be a culture (including cells) or a processed product thereof (such as a mixture of a culture and other components), or a culture-isolated cell (such as a culture).
- When present on plants such as roots, stems, leaves, and seeds, or in soil for cultivation, it has the property of suppressing various plant diseases and controlling pests.
- the D747 strain of the present invention belongs to algal fungi (Oomycetes), ascomycetes (Ascomycetes), basidiomycetes (Basi di omyc etes), and incomplete fungi (Deuteromycetes), depending on the application form. It can control plant diseases caused by fungi and bacteria.
- pathogens examples include, for example, Pseudoperonospora spp.
- ⁇ Botrytis) genus for example Kiyuuri gray mosquito ⁇ fine Fungus Bo tiytis cinerea
- lyso click Nea Rhizoc tonia) genus for example, rice sheath blight fungus Rhizoc tonia solani
- Park Senior p ucc i n i a
- Puccinia recondita Puccinia recondita
- Septoria Septoria
- Sclerotinia Sclerotinia
- ⁇ Burkholderia For example, rice seedling blight bacterial disease Burkholderia plan tarii) can be mentioned, but the present invention is not limited to these examples.
- the D744 strain of the present invention is capable of controlling pests such as Hemiptera pests, Lepidoptera pests, Coleoptera pests, Diptera pests, Orthoptera pests, Termite pests, Azamizuma pests, and Naked pests. Can be controlled.
- Examples of the pests that can be controlled by the strain D 747 include, specifically, Hemiptera pests such as stink bugs (Heteroptera; HETEROPTERA) such as Rip tortus cla va tus, and Nepho te t leafhoppers such as tix cinc ticeps), flying mosquitoes such as flying brown mosquitoes, Nilaparv3 ta lugens), white lice such as Psylla, whiteflies such as silver leaf whitefly Bemisia tabaci), aphids such as white aphids Myzus persicae) Larva
- Hemiptera pests such as stink bugs (Heteroptera; HETEROPTERA) such as Rip tortus cla va tus, and Nepho te t leafhoppers such as tix cinc ticeps
- flying mosquitoes such as flying brown mosquitoes, Nilaparv3 ta lugens
- white lice
- Novels such as (Heliothis virescens), Coleopteran pests, for example,
- Beetles such as (Anomala cuprea), beetles such as Epilachna vigin tioc topunc ta ta, etc .; Diptera, e.g. Culex pipiens), Anopheles sinensis), Culex tri taeniorhynchus), Orthoptera, e.g.
- thistle pests such as Thrips palmi (Sirtothrips dorsal is), mites, and
- the plant disease control agent and the insect pest control agent of the present invention are, as described above, plant diseases and pests.
- the D747 strain can be used alone, but a mutant of the D747 strain can be used together with the D747 strain. .
- the mutant has the bacteriological properties of the above-mentioned D744 strain, has a plant disease controlling action and a pest controlling action, and is a spontaneous mutant, a mutant using ultraviolet light or a chemical mutagen.
- cell fusion strains and genetically modified strains can be used.
- the D747 strain contained in the plant disease control agent and the insect pest control agent includes a mutant of the D747 strain.
- control is used to mean not only the prevention and repelling of diseases and pests but also the elimination and death. Therefore, even in plants already infected with pathogens, by applying the plant disease controlling agent of the present invention, it is possible to remove the pathogens from the plant, it is possible to prevent deterioration of the disease or diseases caused by pathogenic bacteria c Pests can also be controlled by repellent and insecticidal effects.
- D 7 4 7 strain is applied as live bacteria at a concentration of 1 0 5 ⁇ 1 0 1Q number Z m 1, to be added to the plant preferable.
- the timing and amount of application may be appropriately determined according to the case of the viable bacteria described above.
- the D747 strain may be used alone or by diluting with an inert liquid or solid carrier. It may be used as a drug to which an active agent and other auxiliary agents are added. Specific preparation examples include dosage forms such as granules, powders, wettable powders, suspension preparations, and emulsions.
- Examples of preferred carriers include talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, porous solid carriers, water, isopropyl alcohol, xylene, cyclohexanone, Liquid carriers such as methylnaphthalene and alkyl glycol are exemplified.
- surfactants and dispersants include dinaphthyl methansulfonate, alcohol sulfate, alkylaryl sulfonate, Lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, polyoxyethylene sorbitan monoalkylate, and the like.
- the adjuvant include carboxymethylcellulose, polyethylene glycol, propylene glycol, gum arabic and xanthan gum.
- the protective agent include skim milk, pH buffer and the like. In this case, the amount of viable cells of the D747 strain, the amount of Z or its culture, and the timing and amount of application can be appropriately determined according to the case of the viable cells described above.
- the plant disease controlling agent and the insect controlling agent of the present invention can contain other components such as insecticides, other fungicides, herbicides, plant growth regulators, and fertilizers as active ingredients, if necessary.
- the plant disease control agent and the insect pest control agent of the present invention may contain other types of strains in addition to the D747 strain.
- the agent for controlling plant diseases and insect pests of the present invention can be applied directly as it is, or can be applied after being diluted with water or the like.
- the method of applying the plant disease controlling agent and the insect controlling agent is not particularly limited.For example, a method of directly spraying on plants or pests, a method of spraying on soil, a method of adding to water or fertilizer added to plants or soil, etc. Can be exacerbated.
- the application rate of the preparation varies depending on the target disease, target pest, target crop, application method, occurrence tendency, degree of damage, environmental conditions, dosage form to be used, and the like.
- the Bacillus sp. ⁇ Bacillus sp.) D747 strain of the present invention has a broad spectrum of diseases and pests, and can control a plurality of plant diseases and pests.
- the plant disease control agent and pest control agent of the present invention containing the strain D747 of the present invention can control plant diseases and pests, and thus can be used as a biological pesticide. Therefore, the agent for controlling plant diseases and pests of the present invention is highly safe for the environment and has an effect of controlling a plurality of diseases and pests. However, it can widely prevent diseases and pests.
- BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be specifically described with reference to the following examples, but the present invention is not limited to these examples.
- Example 1 Cultivation of D747 strain
- a strain D 747 was isolated from the air in Kikugawa-cho, Ogasa-gun, Shizuoka Prefecture. Specifically, in order to isolate microorganisms floating in the air in Kamo, Kikugawa-cho, Ogasa-gun, Shizuoka Prefecture, remove the lid from the plate medium containing potato dextrose agar medium, leave it for 10 minutes, and let it contact the air. Was. This was cultured at 27 ° C for 3 days, and the formed colony was separated. The isolated colonies were subjected to shaking culture in a potato-dextrose liquid medium, and a strain effective against plant disease bacteria was found. D747 strain was isolated.
- Example 2 Ten rice seeds (variety: Asahi Aichi) were sown in 7.5-cm diameter unglazed pots and grown in a greenhouse. The suspension of the D747 strain obtained as described in Example 1 was sprayed on the rice in which the fourth leaf had spread, at a rate of 10 m1 per pot, and air-dried. Then, the rice blast fungus yricularia oryzae was sprayed. ) Spore suspension was spray-inoculated and maintained in a moist chamber. Five days after the inoculation, the number of lesions on the fourth leaf was examined, and the number of lesions was evaluated based on the criteria shown in Table 1 below. Table 2 shows the test results. Evaluation
- Example 4 Wheat blight control effect test
- Example 5 Wheat powdery mildew control effect test
- Example 6 Test of control effect of cucumber gray mold
- Example 7 Cucumber and disease control effect test
- Example 8 Insecticidal test of rice weevil
- Example 2 30 ml of the suspension of the D747 strain obtained as in Example 1 was placed in a plastic cup having a capacity of 60 ml, and three pieces of rice leaf having a length of 3 cm were floated. After releasing 10 adults of IAssorhop trus oryzophilus), they were capped and left in a constant temperature room at 25 ° C. After 3 days, the number of surviving insects was counted. The test was performed three times, and the mortality was calculated by the following formula (1). Determined by taking the average. Table 3 shows the test results.
- the present invention is characterized by the discovery of a new strain, Bacillus sp. D 747 (FERM BP-8234), and the bacillus sp. )
- Bacillus sp. D 747 (FERM BP-8234)
- the bacillus sp. Alternatively, by virtue of the presence of the viable cells of the culture on plants such as roots, stems, leaves, seeds, or in the cultivation soil, the occurrence of various plant diseases can be controlled in an extremely wide range and the pests can be controlled.
- the plant that has been sprayed with the plant disease controlling agent and the insect controlling agent containing the D747 strain of the present invention has a high controlling effect against various plant diseases and pests.
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Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/495,432 US7094592B2 (en) | 2001-11-26 | 2002-11-26 | Bacillus sp. D747 strain, plant disease controlling agents and insect pest controlling agents using the same and control method using the agents |
| MXPA04004751A MXPA04004751A (es) | 2001-11-26 | 2002-11-26 | Cepa de bacillus sp.d747, agente para control de enfermedades plantas y agente para control de pestes de insector que utiliza la misma y metodo de control que utiliza los agentes. |
| DE60224566T DE60224566T2 (de) | 2001-11-26 | 2002-11-26 | Bacillus sp. d747 stamm, mittel diesen stamm enthaltend zur kontrolle von pflanzenkrankheiten und insektenschädlingen und diese mittel verwendendes bekämpfungsverfahren |
| AU2002355037A AU2002355037A1 (en) | 2001-11-26 | 2002-11-26 | Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents |
| KR1020047007871A KR100903253B1 (ko) | 2001-11-26 | 2002-11-26 | 바실러스 sp. D747 균주, 이를 이용한 식물병해방제제및 해충방제제 및 이들을 이용한 방제방법 |
| EP02788659A EP1449914B1 (en) | 2001-11-26 | 2002-11-26 | Bacillus sp.d747 strain, plant disease controlling agents and insect pest controlling agents using the same and control method using the agents |
| CA2467161A CA2467161C (en) | 2001-11-26 | 2002-11-26 | Bacillus sp. d747 strain, plant disease control agent and insect pest control agent using the same, and control method using the agents |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001359222 | 2001-11-26 | ||
| JP2001-359222 | 2001-11-26 | ||
| JP2002-133294 | 2002-05-08 | ||
| JP2002133294A JP4071036B2 (ja) | 2001-11-26 | 2002-05-08 | バシルスsp.D747菌株およびそれを用いた植物病害防除剤および害虫防除剤 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2003046157A1 true WO2003046157A1 (en) | 2003-06-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2002/012319 Ceased WO2003046157A1 (en) | 2001-11-26 | 2002-11-26 | Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7094592B2 (https=) |
| EP (1) | EP1449914B1 (https=) |
| JP (1) | JP4071036B2 (https=) |
| KR (1) | KR100903253B1 (https=) |
| CN (1) | CN100419070C (https=) |
| AU (1) | AU2002355037A1 (https=) |
| BR (1) | BR0214398B1 (https=) |
| CA (1) | CA2467161C (https=) |
| DE (1) | DE60224566T2 (https=) |
| ES (1) | ES2297031T3 (https=) |
| MX (1) | MXPA04004751A (https=) |
| TW (1) | TWI302942B (https=) |
| WO (1) | WO2003046157A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005082149A1 (ja) * | 2004-02-27 | 2005-09-09 | Itsuki Co., Ltd. | バチルス属細菌を用いた植物病害の防除方法および防除剤 |
| CN100566574C (zh) * | 2004-02-27 | 2009-12-09 | 株式会社树 | 用芽孢杆菌属细菌防治植物病害的方法和防治剂 |
| CN115109723A (zh) * | 2022-06-23 | 2022-09-27 | 湖北工业大学 | 一株卡氏芽孢杆菌st-1及其在香梨病害防治或贮藏保鲜中的应用 |
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| TW535454B (en) | 1999-10-21 | 2003-06-01 | Semiconductor Energy Lab | Electro-optical device |
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- 2002-05-08 JP JP2002133294A patent/JP4071036B2/ja not_active Expired - Lifetime
- 2002-11-22 TW TW091134016A patent/TWI302942B/zh not_active IP Right Cessation
- 2002-11-26 BR BRPI0214398-4B1A patent/BR0214398B1/pt active IP Right Grant
- 2002-11-26 WO PCT/JP2002/012319 patent/WO2003046157A1/ja not_active Ceased
- 2002-11-26 EP EP02788659A patent/EP1449914B1/en not_active Expired - Lifetime
- 2002-11-26 KR KR1020047007871A patent/KR100903253B1/ko not_active Expired - Lifetime
- 2002-11-26 DE DE60224566T patent/DE60224566T2/de not_active Expired - Lifetime
- 2002-11-26 ES ES02788659T patent/ES2297031T3/es not_active Expired - Lifetime
- 2002-11-26 MX MXPA04004751A patent/MXPA04004751A/es active IP Right Grant
- 2002-11-26 US US10/495,432 patent/US7094592B2/en not_active Expired - Lifetime
- 2002-11-26 CN CNB02823250XA patent/CN100419070C/zh not_active Expired - Lifetime
- 2002-11-26 CA CA2467161A patent/CA2467161C/en not_active Expired - Lifetime
- 2002-11-26 AU AU2002355037A patent/AU2002355037A1/en not_active Abandoned
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005082149A1 (ja) * | 2004-02-27 | 2005-09-09 | Itsuki Co., Ltd. | バチルス属細菌を用いた植物病害の防除方法および防除剤 |
| CN100566574C (zh) * | 2004-02-27 | 2009-12-09 | 株式会社树 | 用芽孢杆菌属细菌防治植物病害的方法和防治剂 |
| CN115109723A (zh) * | 2022-06-23 | 2022-09-27 | 湖北工业大学 | 一株卡氏芽孢杆菌st-1及其在香梨病害防治或贮藏保鲜中的应用 |
| CN115109723B (zh) * | 2022-06-23 | 2023-06-23 | 湖北工业大学 | 一株卡氏芽孢杆菌st-1及其在香梨病害防治或贮藏保鲜中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE60224566T2 (de) | 2008-12-24 |
| CA2467161A1 (en) | 2003-06-05 |
| US7094592B2 (en) | 2006-08-22 |
| DE60224566D1 (de) | 2008-02-21 |
| CN1589323A (zh) | 2005-03-02 |
| MXPA04004751A (es) | 2004-08-02 |
| US20040265292A1 (en) | 2004-12-30 |
| EP1449914B1 (en) | 2008-01-09 |
| BR0214398A (pt) | 2004-11-03 |
| KR100903253B1 (ko) | 2009-06-17 |
| AU2002355037A1 (en) | 2003-06-10 |
| JP2003219863A (ja) | 2003-08-05 |
| CA2467161C (en) | 2010-08-17 |
| ES2297031T3 (es) | 2008-05-01 |
| EP1449914A1 (en) | 2004-08-25 |
| CN100419070C (zh) | 2008-09-17 |
| BR0214398B1 (pt) | 2013-08-27 |
| TWI302942B (en) | 2008-11-11 |
| TW200300655A (en) | 2003-06-16 |
| EP1449914A4 (en) | 2006-03-08 |
| KR20040066841A (ko) | 2004-07-27 |
| JP4071036B2 (ja) | 2008-04-02 |
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