WO2002064624A2 - Improved method for production of secreted proteins in fungi - Google Patents

Improved method for production of secreted proteins in fungi Download PDF

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Publication number
WO2002064624A2
WO2002064624A2 PCT/FI2002/000116 FI0200116W WO02064624A2 WO 2002064624 A2 WO2002064624 A2 WO 2002064624A2 FI 0200116 W FI0200116 W FI 0200116W WO 02064624 A2 WO02064624 A2 WO 02064624A2
Authority
WO
WIPO (PCT)
Prior art keywords
protein
promoter
secretable
regulation
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI2002/000116
Other languages
English (en)
French (fr)
Other versions
WO2002064624A3 (en
WO2002064624A8 (en
Inventor
Tiina Pakula
Markku Saloheimo
Jaana Uusitalo
Anne Huuskonen
Adrian Watson
David Jeenes
David Archer
Merja Penttilae
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VTT Technical Research Centre of Finland Ltd
Original Assignee
VTT Technical Research Centre of Finland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VTT Technical Research Centre of Finland Ltd filed Critical VTT Technical Research Centre of Finland Ltd
Priority to CA002438356A priority Critical patent/CA2438356A1/en
Priority to EP02700285A priority patent/EP1360196A2/en
Priority to AU2002233373A priority patent/AU2002233373B2/en
Priority to JP2002564953A priority patent/JP4302985B2/ja
Priority to US10/467,710 priority patent/US20040115790A1/en
Publication of WO2002064624A2 publication Critical patent/WO2002064624A2/en
Publication of WO2002064624A3 publication Critical patent/WO2002064624A3/en
Publication of WO2002064624A8 publication Critical patent/WO2002064624A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
PCT/FI2002/000116 2001-02-13 2002-02-13 Improved method for production of secreted proteins in fungi Ceased WO2002064624A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002438356A CA2438356A1 (en) 2001-02-13 2002-02-13 Improved method for production of secreted proteins in fungi
EP02700285A EP1360196A2 (en) 2001-02-13 2002-02-13 Improved method for production of secreted proteins in fungi
AU2002233373A AU2002233373B2 (en) 2001-02-13 2002-02-13 Improved method for production of secreted proteins in fungi
JP2002564953A JP4302985B2 (ja) 2001-02-13 2002-02-13 真菌における分泌タンパク質の産生のための改良法
US10/467,710 US20040115790A1 (en) 2001-02-13 2002-02-13 Method for production of secreted proteins in fungi

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20010272A FI120310B (fi) 2001-02-13 2001-02-13 Parannettu menetelmä erittyvien proteiinien tuottamiseksi sienissä
FI20010272 2001-02-13

Publications (3)

Publication Number Publication Date
WO2002064624A2 true WO2002064624A2 (en) 2002-08-22
WO2002064624A3 WO2002064624A3 (en) 2002-11-21
WO2002064624A8 WO2002064624A8 (en) 2003-11-27

Family

ID=8560341

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI2002/000116 Ceased WO2002064624A2 (en) 2001-02-13 2002-02-13 Improved method for production of secreted proteins in fungi

Country Status (7)

Country Link
US (1) US20040115790A1 (enExample)
EP (1) EP1360196A2 (enExample)
JP (1) JP4302985B2 (enExample)
AU (1) AU2002233373B2 (enExample)
CA (1) CA2438356A1 (enExample)
FI (1) FI120310B (enExample)
WO (1) WO2002064624A2 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006506980A (ja) * 2002-09-10 2006-03-02 ジェネンコー・インターナショナル・インク 高濃度糖類混合物を用いた遺伝子発現の誘発
WO2008079228A1 (en) * 2006-12-20 2008-07-03 Danisco Us, Inc., Genencor Division Assays for improved fungal strains
CN108192919A (zh) * 2018-02-01 2018-06-22 中国农业大学 一种培育抗旱转基因棉花的方法
US20220025423A1 (en) * 2018-11-28 2022-01-27 Novozymes A/S Modified Filamentous Fungal Host Cells

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CA2575319C (en) * 2004-07-27 2014-10-14 Unilever Plc Aerated food products containing hydrophobin
JP5631533B2 (ja) * 2004-12-23 2014-11-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 遺伝子発現技術
MX2007012954A (es) * 2005-04-20 2008-01-11 Wyeth Corp Sistemas de expresion en mamiferos.
BRPI0715360B8 (pt) 2006-07-27 2022-01-04 Wyeth Corp método para a produção de uma proteína recombinante; método para a produção de uma proteína 2086 meningocócica recombinante (p2086); e composição
WO2009158627A2 (en) * 2008-06-27 2009-12-30 Edeniq, Inc. Cellulosic protein expression in yeast
WO2010111208A1 (en) * 2009-03-23 2010-09-30 University Of Miami Mitochondrial inhibitors and uses thereof
AT510299B1 (de) * 2010-12-22 2012-03-15 Univ Wien Tech Verfahren und mittel zur herstellung von n-acetylneuraminsäure (neunac)
WO2019003180A1 (en) * 2017-06-29 2019-01-03 Savitribai Phule Pune University IMPROVED PRODUCTION AND USES OF A SELF-ASSEMBLY PROTEIN SECRETED BY AN NATIVE YARROWIA LIPOLYTICA STRAIN
CN113528492B (zh) * 2021-09-07 2022-07-22 山东大学 一种将木质纤维素水解液回用于发酵生产纤维素酶液的方法

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006506980A (ja) * 2002-09-10 2006-03-02 ジェネンコー・インターナショナル・インク 高濃度糖類混合物を用いた遺伝子発現の誘発
US9034628B2 (en) 2002-09-10 2015-05-19 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
WO2008079228A1 (en) * 2006-12-20 2008-07-03 Danisco Us, Inc., Genencor Division Assays for improved fungal strains
CN108192919A (zh) * 2018-02-01 2018-06-22 中国农业大学 一种培育抗旱转基因棉花的方法
CN108192919B (zh) * 2018-02-01 2020-08-18 中国农业大学 一种培育抗旱转基因棉花的方法
US20220025423A1 (en) * 2018-11-28 2022-01-27 Novozymes A/S Modified Filamentous Fungal Host Cells

Also Published As

Publication number Publication date
JP2004526440A (ja) 2004-09-02
WO2002064624A3 (en) 2002-11-21
JP4302985B2 (ja) 2009-07-29
EP1360196A2 (en) 2003-11-12
US20040115790A1 (en) 2004-06-17
AU2002233373B2 (en) 2007-11-15
CA2438356A1 (en) 2002-08-22
FI20010272A0 (fi) 2001-02-13
WO2002064624A8 (en) 2003-11-27
FI120310B (fi) 2009-09-15

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