WO2001015799A1 - Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore - Google Patents
Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore Download PDFInfo
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- WO2001015799A1 WO2001015799A1 PCT/FR2000/002376 FR0002376W WO0115799A1 WO 2001015799 A1 WO2001015799 A1 WO 2001015799A1 FR 0002376 W FR0002376 W FR 0002376W WO 0115799 A1 WO0115799 A1 WO 0115799A1
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- solvent
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- coacervation
- active principle
- hardening
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/06—Making microcapsules or microballoons by phase separation
- B01J13/08—Simple coacervation, i.e. addition of highly hydrophilic material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1641—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poloxamers
- A61K9/1647—Polyesters, e.g. poly(lactide-co-glycolide)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1682—Processes
- A61K9/1694—Processes resulting in granules or microspheres of the matrix type containing more than 5% of excipient
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4841—Filling excipients; Inactive ingredients
- A61K9/4866—Organic macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5089—Processes
Definitions
- the present invention relates to a method of microencapsulation of an active principle by coacervation, used in particular for preparing dosage forms with sustained release.
- Microencapsulation techniques are conventionally used to separate incompatible chemical substances, convert liquids into powders, improve the bioavailability of active ingredients, mask the unpleasant taste or odor of certain compounds and prepare sustained-release dosage forms.
- Prolonged release dosage forms are likely to be administered subcutaneously or intramuscularly, and to be found directly in the blood stream or near the organ to be treated so that biodegradable polymers are often chosen to enter their composition.
- Prolonged-release systems based on biodegradable polymers can be administered parenterally, without removal by surgical intervention, since biodegradable polymers are transformed in the body into metabolites eliminated by natural routes.
- the active principle is released, according to a kinetics modulated by the diffusion of the active principle and the process of degradation of the polymer.
- patient compliance is improved due to less frequent administration.
- biodegradable polymers of frequent use in the encapsulation of active principles include the poiy ( ⁇ -hydroxy acids), in particular the polymers of lactic acid (PLA) and the polymers of lactic and glycolic acids (PLAGA), poly- ⁇ -caprolactone, polyorthoesters such as Chronomer ® and Alzamer ® , polyanhydrides, in particular the copolymer of sebacic acid and (carboxyphenoxy) propane, polypeptides and natural biodegradable polymers, such as albumin, bovine serum albumin, collagen and chitosan.
- poiy ( ⁇ -hydroxy acids) in particular the polymers of lactic acid (PLA) and the polymers of lactic and glycolic acids (PLAGA), poly- ⁇ -caprolactone, polyorthoesters such as Chronomer ® and Alzamer ® , polyanhydrides, in particular the copolymer of sebacic acid and (carboxyphenoxy) propane, polypeptides and natural biode
- solvent-free techniques such as spray-congeaimg, extrusion (coextrusion / spheronization), gelification, p ⁇ lling and precipitation of superc ⁇ tic solutions (RESS)
- solvents such as nebulization, coacervation, emulsion-evaporation, emulsion-extraction, with their variants starting from double water / oil / water emulsions
- solvent-free techniques such as spray-congeaimg, extrusion (coextrusion / spheronization), gelification, p ⁇ lling and precipitation of superc ⁇ tic solutions (RESS)
- solvents such as nebulization, coacervation, emulsion-evaporation, emulsion-extraction, with their variants starting from double water / oil / water emulsions
- the conventional microencapsultation techniques (coacervation, emulsion-evaporation) essentially use chlorinated solvents such as dichloromethane (class 2 solvent according to the ICH standard, ie solvent to be limited) as solvent for the polymer Or it is a solvent chlorine known for its neurotoxicity
- chlorinated solvents such as dichloromethane (class 2 solvent according to the ICH standard, ie solvent to be limited)
- dichloromethane class 2 solvent according to the ICH standard, ie solvent to be limited
- the residual level of dichloromethane authorized in the finished product is 600 ppm according to the ICH4 standard
- microparticles obtained contain quantities of residual solvents. It therefore appears necessary to develop new microencapsulation methods which do not use chlorinated solvents. Two major solutions are presented to solve this problem.
- a solution allowing encapsulation without chlorinated solvents is based on methods which do not use any solvent, but certain polymers cannot be used according to these methods. Furthermore, the properties of the particles obtained by these methods do not necessarily correspond. the requirements of long-term treatment
- the present invention provides a method of microencapsulation by coacervation which does not use any chlorinated solvent. More specifically, the invention relates to a coacervation process by adding non-solvent. Coacervation by addition of non-solvent requires the use of three miscible solvents; one of these three solvents is solvent for the polymer and the other two are non-solvent for the polymer.
- the principle of coacervation is based on the controlled desolvation of a polymer dissolved in an organic solvent containing an active principle generally in particulate form, induced by the addition of a non-solvent or coacervation agent for the polymer.
- the solubility of the polymer in the organic solvent is lowered and two immiscible phases are formed: the coacervate is gradually deposited on the surface of the active principle.
- the addition of a hardener allows the formation of a continuous film of polymer around the active ingredient.
- the active ingredient particles can be liquid or solid.
- the active ingredient can also be initially dissolved in the solvent for the polymer. It then reprecipitates in particulate form when the coacervation agent is added, or can form a homogeneous solid solution in the polymer particles resulting from the coacervation.
- the solvent must dissolve the polymer, it is preferred that it does not dissolve the active principle, although the process is still usable with an active principle soluble in the solvent of the polymer
- the coacervation agent must be miscible with the solvent of the polymer II must not be a solvent for the polymer, because otherwise this would amount to a simple transfer of the polymer from the solvent to the coacervation agent. In addition, it must not dissolve the active ingredient to limit the encapsulation losses.
- the hardener must be partially miscible with the polymer solvent to facilitate its extraction. II must not dissolve either the polymer or the active principle, because otherwise the encapsulation yield would be greatly reduced
- the coacervation technique uses dichloromethane or chloroform as the polymer solvent, a silicone oil as a coacervation agent, and heptane as a hardener.
- the present invention relates to a method of microencapsulation of an active principle by coacervation which consists of
- the hardening agent is chosen from water, the alcohols comprising 1 to 4 carbon atoms provided that the hardening agent is an alcohol different from the non-solvent, and their mixtures.
- N-methylpyrrolidone is class 2 like dichloromethane, its limit concentration is much higher (4840 ppm against 600 ppm for dichloromethane).
- the non-solvent and the hardener are respectively chosen from the following pairs: propane-1, 2-diol and propan-2-ol, glycerol and propane-1, 2-diol, glycerol and propan-2-ol , propan-2-ol and propane- 1, 2-diol.
- the polymer is a biodegradable polymer whose molecular weight by weight (Mw) is between 10,000 and 90,000 g / mole, preferably between 15,000 and 50,000 g / mole, the index of which polydispersity (Ip) is between 1 and 3.5, preferably between 1.5 and 2.5.
- Mw molecular weight by weight
- Ip polydispersity
- the polymer is a lactic acid polymer (PLA) or a copolymer of lactic and glycolic acids (PLAGA).
- the polymer is a PLAGA such that Mw is between 15,000 and 20
- Ip is between 1 and 2, of preferably equal to 1.6, and the percentage of glycolic acid is less than 30%, preferably equal to 25%.
- the polymer concentration in the solvent is between 1 and 10% (w / v), preferably of the order of 4% (w / v)
- the non-solvent / solvent volume ratio is between 1/2 and 1 / jl.
- the coacervation temperature is lower than the glass transition temperature of the polymer, preferably less than or equal to 25 ° C, preferably less than 4 ° C, more preferably still less than or equal to - ⁇ ° C
- the non-solvent is added in successive doses of 200 ⁇ l to 1 ml.
- Coacervation is carried out with stirring, for example with magnetic stirring, at a speed between 200 and 1000 rpm.
- the curing agent further contains a surfactant, the concentration of said surfactant in the curing agent being between 0.5 and 10% (v / v) surfactant is a sorbitan ester, e.g. Tween ® 80, or polyvinyl alcohol
- the volume ratio hardener / solvent is between 5/1 and 180/1, and preferably between 15/1 and 120/1
- the hardening of the microspheres is carried out with stirring, for example magnetic, at a speed of between 500 and 1500 rpm
- the curing temperature is less than or equal to 25 ° C, preferably less than 4 ° C, more preferably less than or equal to 0.5 ° C
- the hardener is added several times, preferably at least four times.
- the hardening lasts between 2 and 4 hours.
- microparticles obtained after hardening are filtered on a Millipore ® system, by cent ⁇ fugation or on pleated paper
- the solvent and the non-solvent have a viscosity high enough to stabilize the active principle
- the particle size of the active ingredient is between 1 and 50 microns, and preferably between 5 ⁇ m and 30 ⁇ m
- the solvent is N-methylpyrrolidone
- the non-solvent is ethanol
- the hardener is water
- the solvent is ethyl acetate
- the non-solvent is propan-2-ol
- the hardener is water.
- the polymer is a PLAGA 75.25 as Mw is included. between 15,000 and 20,000, preferably equal to 17,500, Ip is between 1 and 2, preferably equal to 1, 6
- the solvent is J-acetic acid
- the hardener is l water
- the polymer is a 50:50 PLAGA.
- the active principle when the active principle is insoluble in the solvent for the polymer, either a suspension or an emulsion is produced.
- the active principle is ground in a mortar and then suspended in the solvent.
- the suspension can be homogenized with magnetic stirring, the coacervation is then also carried out with magnetic stirring.
- the suspension can also be homogenized with mechanical stirring, at variable speed ( propeller agitator, Heidolph RGL500, Prolabo, Pans, France) or using an Ultra-Turrax ® T25 (Prolabo, Pans, France) In the latter two cases, coacervation is then carried out with mechanical stirring
- the dispersion of the active principle in the polymer solution can also be conventionally carried out with ultrasonic agitation.
- the active principle is water-soluble
- to prepare the emulsion the active principle is dissolved in water, then a water / solvent emulsion of the polymer. is carried out with mechanical stirring
- the coacervation is then carried out with mechanical stirring
- the polymer is a biodegradable polymer of frequent use in the encapsulation of active p ⁇ ncipes, preferably a PL ⁇ or a PLAGA, more preferably a PLAGA of molar mass by weight (Mw) comprised between 10 000 and 90 000, of molar mass in number (Mn) comprised between 4,000 and 40,000, of polydispersity index (Ip) comprised between 1 and 3.5, and the proportion of glycohde of which is comprised between 10 and 60%
- Mw molar mass by weight
- Mn molar mass in number
- Ip polydispersity index
- Polymers with a high molar mass greater than or equal to 15000 g / mole will be preferred since they make it possible to increase the production yield by increasing the volume of the coacervate phase.
- PLAGA is for example Resomer ® RG 502 (Boehringer Ingelheim,
- the concentration of polymer in the solvent must be sufficient to increase the viscosity of the medium, which makes it possible to stabilize the dispersed droplets of coacervate and to limit their aggregation, on the one hand, and to reduce the formation of small microparticles, d 'somewhere else.
- the polymer concentration is preferably between 1 and 10%
- the viscosities of the solvent and of the non-solvent must be sufficient to stabilize the coacervate droplets.
- the volume of non-solvent to be added is defined so as to bring the system into the stability window and obtain a stable coacervate.
- the volume of non-solvent also depends on the concentration of the crystals of active principle in suspension in the organic polymer solution.
- the speed of addition of the non-solvent is sufficiently low to avoid the formation of a large number of microparticles which are too small, i.e. of size between 1 and 2 ⁇ m.
- the slower the phase separation the more uniform the particle size distribution of the microparticles and the smoother the surface of the microparticles.
- the addition of non-solvent is preferably done gradually in doses of 200 ⁇ l to 1 ml, observing at least one minute between each dose.
- a decrease in the speed of agitation during the coacervation step increases the size of the coacervate droplets and then of the final microparticles. But below a limit speed, variable depending on the systems, the kinetics of deposition of the coacervate is too slow and / or the coacervate droplets are too large and are not stable enough. Mechanical or magnetic agitation between 200 rpm and 1000 rpm often gives good results.
- Temperature is the essential parameter of coacervation; it must be lower than the glass transition temperature of the polymer. The lower it is, the more viscous the medium and the less the microparticles tend to aggregate.
- the ideal hardener should not dissolve either the active ingredient or the polymer. It should easily remove the solvent from the polymer.
- the hardener used is water optionally added with surfactant or an alcohol. Water advantageously makes it possible to easily extract the solvent from the polymer. It also has the advantage of being low cost, of not requiring reprocessing of the effluents. However, water is not the ideal hardener, in the case of water-soluble active ingredients because any prolonged contact is responsible for the diffusion of the active ingredient which results in a low degree of encapsulation.
- the encapsulated active principle When the encapsulated active principle is hydrophilic during hardening, it is quickly dissolved by water which penetrates into the microparticles, and can diffuse back out of the particles. By working at low temperature, the diffusion phenomena are reduced, therefore the leakage of active principle towards the aqueous phase, and the encapsulation yield is improved.
- the surfactant or the alcohol make it possible to limit the aggregation of the microparticles together so as to form a homogeneous dispersion. They were selected based on their safety.
- the surfactants are chosen from those frequently used in formulations intended for the injectable route. such as polyoxyethylenated sobitan esters, such as Tween ® 80 and Tween ® 20 (hydrophilic surfactants)
- Montanox ® 80 polyoxyethylene sorbitan monooieate
- Montanox ® 80 polyoxyethylene sorbitan monooieate
- Montane ® 80 (sorbitan oleate) is its equivalent in the range of hpophile surfactants
- Solutol ® HS 15 polyethylene glycol 660 hydroxystearate
- Synperonic ® PE F 68 (Poloxamer 188) is a block copolymer of polyoxyethylene and poiyoxypropylene
- polyvinyl alcohol was used in two different grades: Mowiol ® 4/88 and Rhodov ⁇ ol ® 4/125
- the volume of hardener raised from a compromise II must be sufficient to rapidly remove the solvent from the microspheres, but it must limit the diffusion of the active principle outside the microspheres
- the volume is defined according to the solubility cntomme of the solvent in the external phase , so that the final concentration of the solvent in the hardener is lower than the saturation concentration of the external phase in solvent.
- the ratio of the volume of the hardener to the volume of the solvent is between 5/1 and 180/1, and preferably between 15/1 and 120/1
- Combinations using acetic acid or N-methylpyrrolidone as solvent for the polymer require little hardening agent to give solid microspheres, so that the ratio of the volume of the hardener to the volume of the solvent is advantageously of the order of 5 / 1 in this case
- the method of drying the microparticles is a function of the rigidity of the microspheres, the size and the volumes to be treated
- the tendency of microparticles to aggregate after drying depends on their hydration rate and the amounts of residual solvent
- the solvent for the polymer is ethyl acetate
- the non-solvent is propan-2-ol
- the hardener is a water / surfactant mixture, optionally a water / surfactant / alcohol mixture
- the polymer is preferably a PLAGA 75 25
- the polymer concentration is between 1 and 5% (w / v), preferably equal to approximately 4% (w / v)
- the coacervation is carried out at temperature ambient, preferably at a temperature below 4 ° C, more preferably less than - 4 ° C, with mechanical stirring, preferably at
- the surfactant concentration is between 1 and 10% (v / v).
- the surfactant is Tween ® 80.
- the alcohol is advantageously propan-2-ol or propane-1, 2-diol.
- the aqueous hardener solution is added at least four times.
- the hardening lasts at least 2.5 hours and at most 4 hours, it is carried out at room temperature, preferably at a temperature below 4 ° C, more preferably at 0.5 C C, with mechanical stirring (500 rpm).
- the non-solvent / solvent volume ratio is equal to u.
- the hardener / solvent volume ratio is 120/1.
- the solvent is N-methylpyrrolidone
- the non-solvent is ethanol
- the hardener is a water / surfactant mixture.
- the polymer concentration is between 4 and 10% (w / v). Coacervation and hardening are carried out at room temperature, with magnetic stirring.
- the surfactant concentration is between 0.5 and 10% (v / v).
- the curing time is between 2 and 4 hours.
- the hardener / solvent volume ratio is 40/1.
- the polymer is preferably Resomer RG ® 502 or resomer RG ® 756.
- Example 1 Evaluation of the polymer / solvent / coacervation agent / hardener associations.
- the screening of the solvent / non-solvent / hardener associations is carried out in scintillation vials, on small volumes of organic solutions of polymers 1 or 4 (for N-methylpyrrolidone and PLAGA 50 50 exclusively)% (m / v ), 5 ml of organic polymer solution are placed in a scintillation vial.
- the coacervation agent is then added until a haze persisting in agitation which is characteristic of the formation of coacervate The coacervate is observed at this step in optical microscopy
- 1 ml of this mixture is poured into 10 ml of an aqueous solution of surfactant The presence or absence of microspheres is observed by optical microscopy
- PLAGA 50 50 is soluble in toluene, propan-2-ol, glycerol, dioctyladipate, propane-1,2-d ⁇ ol, xylene, diethylcarbonate and methyl ethyl ketone Associations for which the formation of d 'a coacervate and microspheres are as follows
- Acetic acid can be used as a solvent for the polymer and give rise to the formation of coacervate and well individualized microspheres, provided that water is used as hardener.
- coacervate droplets are formed in the association, acetic acid / propan-2-ol but in the presence of methyl ethyl ketone or propane-1,2-diol as hardeners, the coacervate droplets do not retain their spherical shape and it clusters of polymers are formed.
- Propylene carbonate is an interesting solvent by its low toxicity but also by its partial solubility in water.
- the propylene carbonate / propan-2-ol association makes it possible to dispense with water thanks to the possibility of replacing the external phase with a solvent or at least limiting the diffusion of the active principle, by adding an electrolyte in the external phase or by mixing water and a solvent.
- PLAGA 75: 25 The solvents in which PLAGA 75: 25 is soluble are ethyl acetate, acetone, acetonitrile, acetic acid, dimethyliacetamide, dimethylformamide, diethyl ether, methyl ethyl ketone, and N- methylpyrrolidone.
- PLAGA 75: 25 is insoluble in toluene, propan-2-ol, glycerol, propane-1,2-diol, propylene carbonate, dioctyladipate and triethyl citrate.
- the associations for which the formation of individual microspheres is observed are: - ethyl acetate / propane-1, 2-diol / water + Tween ® 80 or propan-2-ol,
- PLAGA 85: 15 is soluble in propan-2-ol, glycerol, propane-1, 2-diol, dioctyladipate and xylene.
- the associations for which the formation of coacervate and microspheres are observed are:
- Example 2 Realization of microspheres not loaded with active principle by varying the parameters of the coacervation.
- the operating conditions are established for the preparation of microspheres without active principle, in order to produce particles of the desired size.
- the influence of various factors such as the volume of coacervation agent added, the volume of hardener, the type and speed of agitation, and the method of collecting microspheres is studied.
- the polymer is dissolved in 50 ml of organic solvent (Beaker No. 1) to give a 1% (w / v) solution.
- the polymer concentration is brought to 4% (w / v) when the solvent is N-methylpyrrolidone.
- the coacervation agent is added until a stable and visible coacervate is obtained.
- the mixture is poured into a hardener solution added with a surfactant (Beccher No. 2), with stirring.
- the microspheres are then recovered by filtration.
- the associations tested are those selected in Example 1.
- PLAGA 50: 50 Resomer ® RG 502.
- Microspheres are obtained under the conditions described in Table 2 with ethyl acetate as solvent. There are three possibilities for stirring the combination ethyl acetate / propan-1, 2- diol / water + Montanox ® 80 or Tween ® 80: agitation by ultrasound, mechanical stirring paddle and stirring magnetic. There are no notable differences in the morphology or size of the microspheres that can justify the choice of one of these methods.
- Example 3 Production of microspheres loaded with 5-fluorouracil (5-FU) as active ingredient.
- the active principle at low concentration forms with the polymer solution a homogeneous dispersion.
- the stability of the dispersion depends on the solvents; thus N-methylpyrrolidone, which is a viscous solvent, leads to better stability of the dispersion and the losses in antimitotic by decantation or adsorption on the walls of the beakers are reduced.
- propane-1,2-diol as a coacervation agent further stabilizes the system by increasing the viscosity of the medium.
- a passage to ultrasound allows to individualize the active ingredient particles.
- the dispersion is homogeneous and we reach encapsulation yields of 70%.
- Example 4 Study of the N-methylpyrrolidone / ethanol / water + surfactant association.
- the initial formulation of the microparticles uses an organic solution of polymer at 4 or 10% (w / v) for a volume of solvent of 5 ml. Coacervation is carried out with magnetic stirring. The non-solvent for the polymer is added using a micropipette, ml by ml. As soon as a solution disorder appears, a sample is taken and observed by light microscopy. One minute after the onset of the disorder, the coacervation medium is poured dropwise into 200 ml of hardener (water + Tween ® 80 or PVA). The hardening of the microparticles is carried out with mechanical agitation. A new observation by microscopy of the microparticles is carried out during hardening.
- hardener water + Tween ® 80 or PVA
- microparticles are filtered under vacuum, or at atmospheric pressure on filter paper if vacuum filtration is impossible (almost immediate clogging of the filter or too long filtration time). Finally, the microparticles are observed just after filtration and after lyophilization.
- microparticles obtained with the NMP / ethanol / water + surfactant combination are, under optical microscopy, spherical, smooth and regular, whatever the stage of the formulation and whatever the type of polymer.
- the filtration after hardening was carried out on filter paper and at atmospheric pressure.
- the size of the microparticles is between 30 and 50 ⁇ m. Numerous microparticles of approximately 5 ⁇ m in diameter are also observed.
- microparticles obtained all have the same appearance in optical microscopy.
- the type of hardener (water, water + Tween ® 80, or water + PVA), for the same PLAGA 50:50 polymer (RG 502) and at room temperature, has no influence on filtration or on the manufacturing yield of microparticles qur remains between 17.5 and 23.8%.
- the curing time, for the same PLAGA 50:50 polymer (RG 502), makes it possible to increase the manufacturing yield by around 10%, at room temperature.
- Example 5 Microspheres loaded with progesterone or budesonide.
- Progesterone and budesonide are hydrophobic active ingredients soluble in ethyl acetate.
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Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT00958741T ATE261338T1 (de) | 1999-08-27 | 2000-08-25 | Verfahren zum einkapseln von aktiven materialien durch koazervierung von polymern in nichtchlorierten organischem lösungsmittel |
DE60008894T DE60008894T2 (de) | 1999-08-27 | 2000-08-25 | Verfahren zum einkapseln von aktiven materialien durch koazervierung von polymern in nichtchlorierten organischem lösungsmittel |
JP2001520202A JP5217070B2 (ja) | 1999-08-27 | 2000-08-25 | 非塩素化有機溶媒中でのポリマーのコアセルベーションによる有効物質のカプセル化方法 |
EP00958741A EP1216091B9 (fr) | 1999-08-27 | 2000-08-25 | Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore |
MXPA02002250A MXPA02002250A (es) | 1999-08-27 | 2000-08-25 | Metodo para encapsular materiales activos mediante la coacervacion de polimeros en solvente organico no clorado. |
IL14831600A IL148316A0 (en) | 1999-08-27 | 2000-08-25 | Method for encapsulating active substances by coacervation of polymers in non-chlorinated organic solvent |
AU70168/00A AU776959B2 (en) | 1999-08-27 | 2000-08-25 | Method for encapsulating active substances by coacervation of polymers in non-chlorinated organic solvent |
CA002382697A CA2382697C (fr) | 1999-08-27 | 2000-08-25 | Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore |
HU0203199A HU228747B1 (en) | 1999-08-27 | 2000-09-25 | Method for encapsulating active substances by coacervation of polymers in non-chlorinated organic solvent |
IL148316A IL148316A (en) | 1999-08-27 | 2002-02-21 | A method of encapsulating active substances by growing polymers in compressed matter in organic solvents which do not contain chlorine |
NO20020881A NO322106B1 (no) | 1999-08-27 | 2002-02-22 | Fremgangsmate for innkapsling av aktive stoffer ved faseseparasjon av polymerer i ikke-klorinerte organiske losemidler |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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FR9910854A FR2797784B1 (fr) | 1999-08-27 | 1999-08-27 | Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore |
FR99/10854 | 1999-08-27 |
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WO2001015799A1 true WO2001015799A1 (fr) | 2001-03-08 |
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PCT/FR2000/002376 WO2001015799A1 (fr) | 1999-08-27 | 2000-08-25 | Procede d'encapsulation de matieres actives par coacervation de polymeres en solvant organique non-chlore |
Country Status (18)
Country | Link |
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EP (1) | EP1216091B9 (fr) |
JP (1) | JP5217070B2 (fr) |
KR (1) | KR100661093B1 (fr) |
CN (1) | CN1317064C (fr) |
AT (1) | ATE261338T1 (fr) |
AU (1) | AU776959B2 (fr) |
CA (1) | CA2382697C (fr) |
DE (1) | DE60008894T2 (fr) |
DK (1) | DK1216091T3 (fr) |
ES (1) | ES2215718T3 (fr) |
FR (1) | FR2797784B1 (fr) |
HU (1) | HU228747B1 (fr) |
IL (2) | IL148316A0 (fr) |
MX (1) | MXPA02002250A (fr) |
NO (1) | NO322106B1 (fr) |
PT (1) | PT1216091E (fr) |
WO (1) | WO2001015799A1 (fr) |
ZA (1) | ZA200201543B (fr) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002049620A3 (fr) * | 2000-12-21 | 2003-05-15 | Inhale Therapeutic Syst | Induced phase transition method for the production of microparticles containing hydrophobic active agents |
US6599627B2 (en) | 2000-12-13 | 2003-07-29 | Purdue Research Foundation | Microencapsulation of drugs by solvent exchange |
US6767637B2 (en) | 2000-12-13 | 2004-07-27 | Purdue Research Foundation | Microencapsulation using ultrasonic atomizers |
JP2004532112A (ja) * | 2001-05-16 | 2004-10-21 | メインラブ | 植物性タンパク質に基づくマイクロカプセル |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2832312B1 (fr) * | 2001-11-21 | 2004-01-16 | Inst Nat Sante Rech Med | Procede de preparation de microparticules sans solvant toxique, microparticules obtenues selon ce procede, utilisations et compositions pharmaceutiques |
AU2008363816B2 (en) | 2008-11-10 | 2012-12-06 | Colgate-Palmolive Company | Shelf stable capsules |
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EP0330180A1 (fr) * | 1988-02-24 | 1989-08-30 | Biomaterials Universe, Inc. | Microsphères du type acide polylactique contenant des substances physiologiquement actives et leur procédé de préparation |
US5324520A (en) * | 1988-12-19 | 1994-06-28 | Vipont Pharmaceutical, Inc. | Intragingival delivery systems for treatment of periodontal disease |
WO1995013799A1 (fr) * | 1993-11-19 | 1995-05-26 | Alkermes Controlled Therapeutics Inc. Ii | Preparation de microparticules biodegradables contenant un agent biologiquement actif |
WO1997041837A2 (fr) * | 1996-05-07 | 1997-11-13 | Alkermes Controlled Therapeutics Inc. Ii | Microparticules |
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US4389330A (en) * | 1980-10-06 | 1983-06-21 | Stolle Research And Development Corporation | Microencapsulation process |
JPS5966425A (ja) * | 1982-10-08 | 1984-04-14 | Mitsui Toatsu Chem Inc | 生体分解性重合体を用いた微小粒子の製造方法 |
JPS62213840A (ja) * | 1986-03-14 | 1987-09-19 | Snow Brand Milk Prod Co Ltd | マイクロカプセルの乾燥方法 |
JPS6365945A (ja) * | 1986-09-05 | 1988-03-24 | Eisai Co Ltd | マイクロカプセルの製造方法 |
US5234520A (en) * | 1987-03-20 | 1993-08-10 | Morgan Adhesives Co. | Method of making an adhesive construction |
JP3114245B2 (ja) * | 1991-06-03 | 2000-12-04 | 藤沢薬品工業株式会社 | 持続性製剤の製造方法 |
DE69329295T2 (de) * | 1992-12-02 | 2001-03-15 | Alkermes Controlled Therapeutics, Inc. | Wachstumhormon enthaltende mikrosphaeren mit kontrollierter freisetzung |
WO1995013814A1 (fr) * | 1993-11-19 | 1995-05-26 | Janssen Pharmaceutica N.V. | 1,2-benzisoxazoles et 1,2-benzisothiazoles microencapsules et substitues par 3-piperidinyle |
US5650173A (en) * | 1993-11-19 | 1997-07-22 | Alkermes Controlled Therapeutics Inc. Ii | Preparation of biodegradable microparticles containing a biologically active agent |
-
1999
- 1999-08-27 FR FR9910854A patent/FR2797784B1/fr not_active Expired - Fee Related
-
2000
- 2000-08-25 WO PCT/FR2000/002376 patent/WO2001015799A1/fr active IP Right Grant
- 2000-08-25 CN CNB008121311A patent/CN1317064C/zh not_active Expired - Fee Related
- 2000-08-25 AU AU70168/00A patent/AU776959B2/en not_active Ceased
- 2000-08-25 DE DE60008894T patent/DE60008894T2/de not_active Expired - Lifetime
- 2000-08-25 AT AT00958741T patent/ATE261338T1/de active
- 2000-08-25 KR KR1020027002467A patent/KR100661093B1/ko not_active IP Right Cessation
- 2000-08-25 CA CA002382697A patent/CA2382697C/fr not_active Expired - Fee Related
- 2000-08-25 MX MXPA02002250A patent/MXPA02002250A/es active IP Right Grant
- 2000-08-25 IL IL14831600A patent/IL148316A0/xx active IP Right Grant
- 2000-08-25 DK DK00958741T patent/DK1216091T3/da active
- 2000-08-25 JP JP2001520202A patent/JP5217070B2/ja not_active Expired - Lifetime
- 2000-08-25 EP EP00958741A patent/EP1216091B9/fr not_active Expired - Lifetime
- 2000-08-25 PT PT00958741T patent/PT1216091E/pt unknown
- 2000-08-25 ES ES00958741T patent/ES2215718T3/es not_active Expired - Lifetime
- 2000-09-25 HU HU0203199A patent/HU228747B1/hu not_active IP Right Cessation
-
2002
- 2002-02-21 IL IL148316A patent/IL148316A/en not_active IP Right Cessation
- 2002-02-22 NO NO20020881A patent/NO322106B1/no not_active IP Right Cessation
- 2002-02-26 ZA ZA200201543A patent/ZA200201543B/en unknown
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EP0330180A1 (fr) * | 1988-02-24 | 1989-08-30 | Biomaterials Universe, Inc. | Microsphères du type acide polylactique contenant des substances physiologiquement actives et leur procédé de préparation |
US5324520A (en) * | 1988-12-19 | 1994-06-28 | Vipont Pharmaceutical, Inc. | Intragingival delivery systems for treatment of periodontal disease |
WO1995013799A1 (fr) * | 1993-11-19 | 1995-05-26 | Alkermes Controlled Therapeutics Inc. Ii | Preparation de microparticules biodegradables contenant un agent biologiquement actif |
WO1997041837A2 (fr) * | 1996-05-07 | 1997-11-13 | Alkermes Controlled Therapeutics Inc. Ii | Microparticules |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6599627B2 (en) | 2000-12-13 | 2003-07-29 | Purdue Research Foundation | Microencapsulation of drugs by solvent exchange |
US6767637B2 (en) | 2000-12-13 | 2004-07-27 | Purdue Research Foundation | Microencapsulation using ultrasonic atomizers |
WO2002049620A3 (fr) * | 2000-12-21 | 2003-05-15 | Inhale Therapeutic Syst | Induced phase transition method for the production of microparticles containing hydrophobic active agents |
EP1343480B2 (fr) † | 2000-12-21 | 2016-02-17 | Alrise Biosystems GmbH | Procede comprenant un transition de phase induite pour la production de microparticules contenant des agents actifs hydrophobes |
JP2004532112A (ja) * | 2001-05-16 | 2004-10-21 | メインラブ | 植物性タンパク質に基づくマイクロカプセル |
Also Published As
Publication number | Publication date |
---|---|
FR2797784A1 (fr) | 2001-03-02 |
AU7016800A (en) | 2001-03-26 |
CA2382697C (fr) | 2009-05-05 |
KR100661093B1 (ko) | 2006-12-22 |
MXPA02002250A (es) | 2003-08-20 |
CA2382697A1 (fr) | 2001-03-08 |
CN1317064C (zh) | 2007-05-23 |
ATE261338T1 (de) | 2004-03-15 |
NO20020881L (no) | 2002-04-25 |
HU228747B1 (en) | 2013-05-28 |
DE60008894T2 (de) | 2005-03-03 |
DE60008894D1 (de) | 2004-04-15 |
KR20020046284A (ko) | 2002-06-20 |
DK1216091T3 (da) | 2004-07-12 |
AU776959B2 (en) | 2004-09-30 |
JP2003508195A (ja) | 2003-03-04 |
CN1371301A (zh) | 2002-09-25 |
EP1216091B9 (fr) | 2005-01-19 |
ZA200201543B (en) | 2002-11-27 |
EP1216091A1 (fr) | 2002-06-26 |
NO20020881D0 (no) | 2002-02-22 |
JP5217070B2 (ja) | 2013-06-19 |
ES2215718T3 (es) | 2004-10-16 |
PT1216091E (pt) | 2004-08-31 |
NO322106B1 (no) | 2006-08-14 |
HUP0203199A2 (en) | 2003-03-28 |
EP1216091B1 (fr) | 2004-03-10 |
IL148316A0 (en) | 2002-09-12 |
FR2797784B1 (fr) | 2001-11-30 |
IL148316A (en) | 2006-10-31 |
HU0203199D0 (fr) | 2002-10-28 |
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