WO1998042204A1 - Agent prophylactique et therapeutique contre les maladies infectieuses chez les poissons et les crustaces - Google Patents

Agent prophylactique et therapeutique contre les maladies infectieuses chez les poissons et les crustaces Download PDF

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Publication number
WO1998042204A1
WO1998042204A1 PCT/JP1998/001145 JP9801145W WO9842204A1 WO 1998042204 A1 WO1998042204 A1 WO 1998042204A1 JP 9801145 W JP9801145 W JP 9801145W WO 9842204 A1 WO9842204 A1 WO 9842204A1
Authority
WO
WIPO (PCT)
Prior art keywords
fish
shellfish
fucoidan
feed
shellfishes
Prior art date
Application number
PCT/JP1998/001145
Other languages
English (en)
Japanese (ja)
Inventor
Yukinori Takahashi
Akio Kanemitsu
Hiroshi Omura
Masato Nagaoka
Sadao Ueyama
Teturo Yamashita
Teruo Yokokura
Original Assignee
Kabushiki Kaisha Yakult Honsha
Miyako Kagaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabushiki Kaisha Yakult Honsha, Miyako Kagaku Kabushiki Kaisha filed Critical Kabushiki Kaisha Yakult Honsha
Priority to KR1019997007938A priority Critical patent/KR20000075860A/ko
Publication of WO1998042204A1 publication Critical patent/WO1998042204A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A01K61/13—Prevention or treatment of fish diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/80—Feeding devices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Definitions

  • the present invention relates to a drug for preventing and treating infectious diseases caused by pathogenic bacteria and viruses of fish and shellfish, a feed containing the same, and a method of cultivating fish and shellfish by feeding the feed.
  • PRDV Penaeid Rod-Shaped DNA Virus infection
  • PRDV Penaeid Rod-Shaped DNA Virus infection
  • Propagated throughout Japan As a result, severe damage has occurred in all areas except Okino, but no effective preventive and therapeutic measures have been established.
  • PRDV the causative virus of this disease
  • the problem is not limited to prawn virus infection, but also other fish and shellfish bacterial infections.
  • fish diseases include iridovirus infections such as red sea bream, puli and amberjack, infectious hematopoietic soil death in salmonids, streptococcal disease in Puri, vibrio disease in Madipuri, and Venedetia in Puri. Parasitosis and the like.
  • antibiotics are mainly administered for bacterial infections, but at present, sufficient effects have not been obtained.
  • the use of antibiotics has led to the emergence of resistant bacteria, and there is concern that they may affect the human body. Therefore, safe alternatives to these substances have been desired.
  • an object of the present invention is to provide a safe substance having a prophylactic and therapeutic effect against infection of viruses, pathogenic bacteria, and parasites, and to improve the survival rate of fish and shellfish in a farm.
  • the present inventors have conducted intensive studies and found that sulfated polysaccharides are effective in preventing and treating infectious diseases of fish and shellfish caused by viruses and pathogenic bacteria.
  • the present invention has been completed.
  • the present invention provides a prophylactic / therapeutic agent for infection of fish and shellfish containing a sulfated polysaccharide as an active ingredient.
  • the present invention also provides a feed for culturing fish and shellfish, which comprises a sulfated polysaccharide.
  • the present invention also provides a method for cultivating fish and shellfish, which comprises feeding the fish culture feed.
  • FIG. 1 is a graph showing the survival rate of prawns after virus infection.
  • FIG. 2 is a diagram showing the survival rate of yellowtail after virus infection.
  • Examples of the sulfated polysaccharide which is the active ingredient of the present invention include fucoidan, rhamnan sulfate, carrageenan and the like.
  • Fucoidan is a sulfated polysaccharide composed mainly of fucose and having a molecular weight of around hundreds of thousands of thousands. It is harmless.
  • Rhamnan sulfate is a sulfated polysaccharide mainly composed of rhamnose and having a molecular weight of about tens of thousands to hundreds of thousands, and is contained in green algae, such as green algae and green grass (Eugomontia sp.).
  • carrageenan is a sulfated polysaccharide having a molecular weight of about hundreds of thousands and containing a major component of galactose, anhydrogalactose, sulfuric acid, etc., and is a red alga, Kirinsai (Euche Kasumi a sp.). , Ibaranori (Hiynea sp.), And Suginori (Gigrt ina sp.).
  • fucoidan has the effect of inhibiting the colonization of Helicobacter pylori, which is believed to be the cause of gastric ulcer, on the gastric mucosa, and as a treatment for preventing gastric ulcer and gastric cancer. Although its use has been reported, its use as a prophylactic and therapeutic agent for fish and shellfish infectious diseases is unknown.
  • fucoidan, rhamnan sulfate, and carrageenan are contained in brown algae, green algae, red algae, etc., these may be fed directly as pulverized or the like.
  • An extraction method has been established for fucoidan, rhamnan sulfate, and carrageenan, and the extract can be suitably used.
  • the extraction method include powers such as an acid extraction method and a hot water extraction method, and are not limited thereto, and can be extracted by various methods.
  • Fucoidan, rhamnan sulfate, and carrageenan are used in the present invention after being reduced in molecular weight or oligosaccharified by hydrolysis of a high molecular weight extract from seaweed, for example, by treatment with a weak acid. Is also good.
  • Fucoidan, rhamnan sulfate and carrageenan may be fed directly with seaweeds or dried extracts containing them, but are preferably used by mixing or blending them with feed materials usually used for aquaculture.
  • the mixing amount when these are mixed or mixed with the feed varies depending on the fish and shellfish and the cultivation method, but in general, it is preferably about 0.5 to 50 g Zkg for prevention of infection. Also, the feed amount of feed containing these should be about 3% by weight of fish and shellfish a day.
  • the fish and shellfishes to which the present invention can be applied are not particularly limited, and include freshwater fish, eel, koi, rainbow trout, va, tilapia, crucian carp, etc.
  • Invertebrates such as crosoy, trough and amberjack include kuruma prawns, black tigers, ⁇ shevids, ⁇ lajevi, and thistles.
  • Infectious diseases that can be applied include viral infections such as iridovirus, birnavirus, and PRDV, and bacterial infections such as vibrio, pseudomonas, streptococci, and syringoid disease.
  • fucoidan The mechanism of infection and treatment by fucoidan, rhamnan sulfate, and carrageenan has not been elucidated yet, but glycolipids and sugar chains on the surface of bacterial viruses play an important role in attaching and infecting cells in the body. .
  • fucose residues of fucoidan are involved in this, and it seems to protect against infection.
  • Test prawns Using shrimp with an average body weight of 8 g obtained from Mitsui Norin, preliminarily reared for one week until the start of the test, and then subjected to experiments.
  • a bottom filtration device was attached to a 60 ⁇ 40 ⁇ 30 cm water tank, and the animals were bred with sufficient airing.
  • test specimens 28 in control group, 26 in fucoidan mixed amount 0.5 g / kg feed group, 26 in fucoidan mixed region 1.0 g / kg feed group 26, fucoidan mixed amount 3
  • Virus was infected on the 8th day after feeding, and the survival rate on the 10th day after the challenge was determined.
  • the infection was carried out by homogenizing the head and thorax of prawns infected with the virus in sterile seawater and then performing high-speed centrifugation. The obtained supernatant was suspended in seawater so as to be diluted about 800-fold.
  • the prawn was put into this solution, and immersed for 2 hours while air aging to infect.
  • the water temperature during the breeding period was 23 degrees.
  • the prawns that died during the experimental period were tested for virus using the PCR method, and confirmed to have died due to the virus.
  • Figure 1 shows the survival rate after infection.
  • Test fish Average fish weight: 400 g.
  • Test fish Average fish weight: 400 g.
  • the animals were preliminarily reared for 14 days before the start of the test and then used for the test.
  • Breeding method A 100 liter circular pan-light aquarium was installed, and the breeding seawater was recycled. Airlanyon was applied sufficiently. Number of test tails: For the test, 150 prey healthy fish (30 tails in each test group) were provided.
  • Feed Control plots use pre-moist pellets, and test plots use a mixture of moist pellets with fucoidan at 1.0, 1.5, 2.0, and 2.5 g / kg, respectively. did. Feeding was performed at 10 o'clock on a daily basis, and the set feeding rate was 44% per body weight of Puri fish.
  • Test method The test feed was administered for 39 consecutive days. Preliminary breeding was performed for 14 days, and on the end of preliminary breeding, a challenge virus infection was carried out with the virus. The test period was set at 25 days, and the survival rate of the pri was determined.
  • challenge infection the iridovirus-infected site is homogenized in sterile seawater, and the supernatant obtained by high-speed centrifugation is diluted 800-fold with seawater to prepare a suspension. A test fish was put into the dish, and immersion was performed for 2 hours while air-rating.
  • the water temperature during the test period was set at 20 ° C, and the presence of iridovirus infection was confirmed by the PCR method for all prids that died during the period. As a result, all the causes of death were due to infection with irid virus.
  • Figure 2 shows the survival rates after infection. As a result, administration of fucoidan was able to prevent dose-dependent death from Puri viridovirus. Industrial applicability
  • infectious diseases of fish and shellfish can be prevented or treated safely and stably, and cultured fish etc. can be stably supplied to the market.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Agent prophylactique et thérapeutique contre les maladies infectieuses chez les poissons et les crustacés contenant en tant qu'ingrédient actif un polysaccharide sulfaté, et aliment de culture des poissons et des crustacés contenant un polysaccharide sulfaté. Ce polysaccharide sulfaté sert à prévenir ou à traiter efficacement les maladies infectieuses chez les poissons et les crustacés.
PCT/JP1998/001145 1997-03-21 1998-03-18 Agent prophylactique et therapeutique contre les maladies infectieuses chez les poissons et les crustaces WO1998042204A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019997007938A KR20000075860A (ko) 1997-03-21 1998-03-18 어패류의 감염증 예방 ·치료제

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP9/67973 1997-03-21
JP6797397 1997-03-21

Publications (1)

Publication Number Publication Date
WO1998042204A1 true WO1998042204A1 (fr) 1998-10-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1998/001145 WO1998042204A1 (fr) 1997-03-21 1998-03-18 Agent prophylactique et therapeutique contre les maladies infectieuses chez les poissons et les crustaces

Country Status (5)

Country Link
KR (1) KR20000075860A (fr)
CN (1) CN1290429C (fr)
ID (1) ID22487A (fr)
TW (1) TW513289B (fr)
WO (1) WO1998042204A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004307346A (ja) * 2003-04-02 2004-11-04 Yakult Honsha Co Ltd 魚類の感染予防・治療剤
CN105247043A (zh) * 2013-05-31 2016-01-13 英特维特国际股份有限公司 鳞片脱落疾病(sdd)致病病毒及其衍生物

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CL2015000876A1 (es) * 2015-04-08 2015-08-28 Maqui New Life S A Composición veterinaria de extracto de algas marinas con al menos 5% de fucoidianos y extracto de planta andrographis sp con al menos 5% de andrografolidos, útil en el control y prevención de infecciones producidas por microorganismos intracelulares en peces.
CN105454722A (zh) * 2015-12-12 2016-04-06 重庆华楷水产养殖有限公司 一种预防鱼类自由基诱发疾病的固体饲料
CN106360139A (zh) * 2016-08-31 2017-02-01 霍山县新元生态农业有限公司 一种草鱼饲料添加剂
WO2021162742A1 (fr) 2020-02-12 2021-08-19 HP Ingredients Corp. Additif alimentaire amélioré pour l'aquaculture
KR102629623B1 (ko) 2020-11-18 2024-01-29 주식회사 에프알에스아이 패류 질병 치료용 마이크로 캡슐, 이의 제조방법 및 이를 포함하는 패류 질병 치료용 조성물

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0379603A (ja) * 1989-08-23 1991-04-04 Zeria Pharmaceut Co Ltd パロチンコンドロイチン硫酸及び該化合物を含有する魚介類の成長促進剤
JPH0670696A (ja) * 1992-06-30 1994-03-15 Senju Pharmaceut Co Ltd 魚介類投与用組成物および魚介類育成用水における水汚濁抑制方法
JPH06181656A (ja) * 1992-12-16 1994-07-05 Ajinomoto Co Inc 魚類・甲殻類のウイルス病予防治療剤及び飼料
JPH0741505A (ja) * 1993-07-27 1995-02-10 Nippon Oil Co Ltd 抗ウイルス物質
JPH07108859B2 (ja) * 1992-06-08 1995-11-22 三菱化学株式会社 魚類の感染症予防用薬剤
JPH07108858B2 (ja) * 1992-06-08 1995-11-22 三菱化学株式会社 魚類の感染症の予防用薬剤
JPH08325158A (ja) * 1995-05-26 1996-12-10 Maruha Corp ウイルス病予防治療剤および飼料
JPH0967265A (ja) * 1995-08-31 1997-03-11 Nippon Oil Co Ltd 魚類病原ウイルスに対する抗ウイルス剤
JPH09206067A (ja) * 1996-02-08 1997-08-12 Kyushu Medical:Kk 甲殻類のウィルス感染症防除剤、この防除剤の甲殻類への投与方法、及びこの防除剤を構成する微生物の培養液
JPH1045600A (ja) * 1996-08-02 1998-02-17 Maruha Corp 甲殻類又は魚類の感染症予防治療剤及びそれを含有する飼料

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0379603A (ja) * 1989-08-23 1991-04-04 Zeria Pharmaceut Co Ltd パロチンコンドロイチン硫酸及び該化合物を含有する魚介類の成長促進剤
JPH07108859B2 (ja) * 1992-06-08 1995-11-22 三菱化学株式会社 魚類の感染症予防用薬剤
JPH07108858B2 (ja) * 1992-06-08 1995-11-22 三菱化学株式会社 魚類の感染症の予防用薬剤
JPH0670696A (ja) * 1992-06-30 1994-03-15 Senju Pharmaceut Co Ltd 魚介類投与用組成物および魚介類育成用水における水汚濁抑制方法
JPH06181656A (ja) * 1992-12-16 1994-07-05 Ajinomoto Co Inc 魚類・甲殻類のウイルス病予防治療剤及び飼料
JPH0741505A (ja) * 1993-07-27 1995-02-10 Nippon Oil Co Ltd 抗ウイルス物質
JPH08325158A (ja) * 1995-05-26 1996-12-10 Maruha Corp ウイルス病予防治療剤および飼料
JPH0967265A (ja) * 1995-08-31 1997-03-11 Nippon Oil Co Ltd 魚類病原ウイルスに対する抗ウイルス剤
JPH09206067A (ja) * 1996-02-08 1997-08-12 Kyushu Medical:Kk 甲殻類のウィルス感染症防除剤、この防除剤の甲殻類への投与方法、及びこの防除剤を構成する微生物の培養液
JPH1045600A (ja) * 1996-08-02 1998-02-17 Maruha Corp 甲殻類又は魚類の感染症予防治療剤及びそれを含有する飼料

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004307346A (ja) * 2003-04-02 2004-11-04 Yakult Honsha Co Ltd 魚類の感染予防・治療剤
CN105247043A (zh) * 2013-05-31 2016-01-13 英特维特国际股份有限公司 鳞片脱落疾病(sdd)致病病毒及其衍生物

Also Published As

Publication number Publication date
KR20000075860A (ko) 2000-12-26
CN1251020A (zh) 2000-04-19
TW513289B (en) 2002-12-11
CN1290429C (zh) 2006-12-20
ID22487A (id) 1999-10-21

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