TW513289B - Prophylactic and therapeutic agent for infectious disease of fishes and shellfishes - Google Patents

Abstract

The invention relates to a prophylactic and therapeutic agent for infectious diseases of fishes and shellfishes, comprising a sulfated polysaccharide as an active ingredient, and a feed for the cultivation of fishes and shellfishes, comprising a sulfated polysaccharide. According to the invention, the infectious diseases of fishes and shellfishes can be safely prevented or treated.

Description

513289 A7

Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Education. 5. Description of the invention (1) Technical Field The present invention relates to the prevention and treatment of infectious diseases caused by pathogens, viruses, and other diseases caused by fish-like organisms. A method of breeding fish feed with these feeds. BACKGROUND At present, a variety of fish species are cultured in various regions of Japan. Among them, there are quite a lot of fish breams such as prawns, yellow-tailed, red sea bream, and bream. Among them, a large number of farmed fish mediums supply market demand. Among these fish slugs, unlike wild fishes, which are fed at high density, they are susceptible to disease. The cause of the disease is mainly due to infection with pathogens, mothers, and parasites. To prevent these diseases, various countermeasures must be adopted. Fish farming begins with the sowing of various fry to fry. Although domestic fish fry are currently used, from the viewpoint of cost, the use of imported fry has increased in recent years. However, a viral infection [infectious disease caused by PRDV (penaeid-like DNA virus)] began to appear in Taiwan, mainland China, and Southeast Asian countries a few years ago, which can cause shrimps and lobsters to be fatal and have been transmitted to Japan by importers. Serious damage was caused in many areas except Osaka. However, effective prevention and treatment measures have not been established. PRDV is the pathogenic virus of this disease. It is a DNA virus with a mantle size of about 400 X 150 μm. The end bones of prawns infected with this virus form multiple white spots with a lethal rate of at least 80%. Because PRDV is a virus, there is no way to directly treat this infectious disease with drugs. Therefore, the only attempt to increase the survival rate of farmed fish (shrimp) by administering immune boosters is currently being attempted. ____ -4- This paper is suitable for financial purposes_ Please read the notes on the back before filling this page) Order

• I 513289 A7 B7 V. Description of the invention (2 Printed by the Consumers Cooperative of the Central Standards Bureau of the Ministry of Disposal, “When this viral disease is in—the fish, prawns, etc. are removed from the fish burial for protection. Sleeve The breeding of fish fry and shrimp fry from 'Wangbuquan' was restarted. After the introduction of females, the female was found to be the mother disease living inside and outside the fish bream. The reason is that in this case, preventive measures against this infectious disease In addition to the infection of Mingyu virus, other fish-borne bacterial infections, etc. are also caused. Fish-borne diseases include infectious diseases caused by Ildovirus (㈣. Virus) in the red mother net, rearing tail, grouper, etc .; salmonid Infectious blood organ necrosis; yellow-tailed streptococcal disease; red sea bream and yellow-tailed mycotic disease and yellow-tailed BenedetM worm disease. Among these fish diseases, bacterial salty infections must be treated with antibiotics. But in In this case, sufficient results cannot be achieved. The use of antibiotics causes the emergence of drug-resistant bacteria and is also afraid of harming human health. Therefore, it is necessary to develop a safe substance that can replace antibiotics. The purpose of the present invention is to provide a kind of virus , Pathogenic bacteria and parasitic infections are safe substances with preventive and curative effects, and improve the survival rate of fishes in fish bream. Disclosure of the invention In view of the situation described above, the inventors have conducted thorough research. As a result, they found that acidified saccharides can be used to prevent and treat fish-borne viruses And the transmission of pathogenic bacteria, which led to the completion of the present invention. Thus, according to the present invention, a preventive and treatment agent for fish-borne infectious diseases is provided, which includes sulfated polysaccharides as an active ingredient. According to the present invention, a feed for farmed fish-media, comprising Sulfate polysaccharide. The main effect of sulfur dye therapy (please read the precautions on the back before filling this page)

、 1T t -5- This paper size is applicable to Chinese National Standard (CNS) A4 specifications (210X 297mm printed by the Ministry of Standards Bureau employee consumer cooperatives 513289 ΑΊ Β7 5. Description of the invention (3) According to the present invention, another A method for cultivating fish medium includes feeding the aforementioned fish medium for feed. Brief description of the drawings. Figure 1 illustrates the survival rate of prawns after being infected with the virus. Figure 2 illustrates the survival rate of yellow tails after being infected with the virus. The best mode can be used for the active ingredients of the examples of the present invention. Examples of sulfated polysaccharides include fucoidan, rhamnyl sulfate and carrageenan. Fucoidan is a sulfated polysaccharide mainly composed of fucose It has a molecular weight of several hundreds of thousands, and it is contained in a large amount in brown algae such as Nemacystus decipiens, Shifan, seagrass ash, Undaria pinnatifida, seaweed, amijigosa (Dictyota sp.), And it is safe for humans. Rhamnan sulfate is mainly composed of rhamnolide Sulfated polysaccharide composed of sugar, and molecular weight of about tens to hundreds of thousands are contained in green algae, hitoegusa (Eugomontia sp.) And other green algae. Carrageenan is a kind of sulfated polysaccharide It contains galactose, dehydrated galactose, sulfuric acid, etc. as the main components, and has a molecular weight of about several hundred thousand, and is contained in red algae such as kirinsai (Eucheuma sp ·), ibaranori (Hiynea sp.), And suginori (Gigartina sp ·). Glycan is said to have the effect of inhibiting the fixation of Helicobacter pylori (which is said to be the cause of gastric ulcer) to gastric mucosa, so it can be used as a therapeutic agent for gastric ulcer and gastric cancer. However, its use as a preventive and therapeutic agent for fish-borne infectious diseases is unknown As fucoidan, rhamnosyl sulfate and carrageenan are contained in brown algae, green algae and red algae respectively, these algae can be fed directly after grinding, etc. It has been known that fucoidan, rhamnose sulfate Extraction method of esters and carrageenan, -6-This paper uses Chinese National Standard (CNS) A4 standard orange (2 丨 0X297 公 #) (Read the precautions on the back before filling this page)

1T • I. V. Description of the invention u Μ 7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Distillation. Examples of extraction methods include acid extraction and hot water extraction. : The extraction method is not limited to these methods, and fucoidan, phyllan sulfate vinegar, and carrageenan can be extracted by any other method by extracting to high molecular weight products of the corresponding algae, such as undergoing weak acid treatment and hydrolysis. And conversion to low molecular weight products or oligosaccharides can also be used in the present invention. ~ Fucoidan 'rhamnose sulfate g and carrageenan can be fed directly as a dried product of algae forms or extracts containing these compounds. However, it is usually better to mix or mix in the breeding feed before use. The mixing ratio of these substances or mixed with feed varies with the species of fish and the breeding method. However, it is generally preferred that the mixing ratio for preventing infection is about 0.5 to 50 g / kg. The feed rate of feeds containing these substances is preferably controlled to about 3% by weight of the weight of fish cultured daily. There are no particular restrictions on the fish species that can be used in the present invention. But its examples include freshwater fish such as bream 'carp, red trout, sweetfish, tirapia and cross carp; saltwater fish such as yellow tail, sea bream, silver salmon, mackerel, mackerel, flounder, kuro 〇〇 (Sebastes sp ·), Fugu rubripes rubripes and groupers; and invertebrate propellants such as prawns' black tigers, Penaeus sp., yellow sea prawns, and crabs. Examples of infectious diseases applicable to the present invention include infectious diseases caused by viruses such as the Eldovirus' Bimavirus and PRDV; and bacterial infectious diseases such as orphan disease, pseudomonasosis, streptococcus disease, and Tuberculosis is ear disease. The mechanism of fucoidan, rhamnosyl sulfate and carrageenan to prevent and treat infections has not been clarified, but it is considered for the following reasons. Existing in the small f's scale, applicable to China National Standard (CNS) A4 (210X 297 male f) (Please read the precautions on the back before filling this page) 丨 锺 _ •• I. 513289 Employee Consumption Cooperative Seal Α7 Β7 V. Description of the Invention (5) Glycolipids and sugar chains on the surface of the virus play an important role when they adhere to the cells of the living body to be infected. Fucose residues such as fucose participate in these glycolipids and sugar chains to prevent infection. Examples The details of the present invention will be described later by the following examples. However, the present invention is not limited to the following examples. Example 1 · Effect of Fucoidan on PRDV Induced Prawn Infectious Diseases Test Prawns: An average weight of 8 grams was purchased from MITSUI NORIN Company. One was initially cultivated before the test and then used for the test. Number of shrimps used in the experiment: 28 shrimps were used in the control group, 26 shrimps were used in the feed culture group containing fucoidan with a mixing ratio of 0.5 g / kg, and 26 shrimps Shrimp was used in a feed culture group using a fucoid-containing feed ratio of 1.0 g / kg, 28 shrimp was used in a feed culture group using a fucoid-containing feed ratio of 3.0 g / kg, and 2 Eight prawns were used in a feed culture group containing a mixture of fucoidan with a ratio of 50 g / kg. The feed was given at the designated time at night, and the feeding rate of each group was set to 2% of the body weight of shrimp. Experimental method: Each feed was continuously fed for 17 days. On the 8th day after feeding, prawns of each group were infected with PRDV, and the survival rate was determined on the 10th day after challenge. Infection is performed as follows. The virus-infected prawn head breast carapace was homogenized in sterile seawater and then centrifuged. The resulting supernatant was suspended in seawater to obtain a 1/8, 000 dilution. Each group was placed in the maggot floating solution, immersed in it and oxygenated for 2 hours at the same time, so that _____________-8-This paper size is applicable to the Chinese national standard ^ TcNS) Publication 1 1 " 一 --- i — -, --0 -I (Please read the notes on the back before filling this page) Order

• I 513289 Printed by the Consumers' Cooperative of the Central Standards Bureau of Didi Ministry A7-__ B7 V. Description of the invention (6) ~ 1 Ming He infected with the virus. The water temperature was maintained at 231 during the cultivation period. As for the prawn that died during the experiment, the PCR method was used to detect the virus, which was confirmed; the blemish was deadly by the virus. An example of survival after infection is shown in Figure j. It was found that the administration of fucoidan could prevent prawn death from prDV and was dose-dependent. Example 2 · Fucoidan is used for testing fish with yellow tail infected by illdovirus: The yellow tail with an average weight of 400 g / yellow fish was initially cultured 4 days before the start of the test and then used for testing. Cultivation method: Build a 1,000-liter round! > Niu 141 ^ tank recycles seawater for aquaculture. Perform aeration. Knife Test Yellow Tail Number: Use 150 healthy yellow tails (30 yellow tails per group). Feed: Yellow-tailed wet pellets were used in the control group, and the mixture of wet pellets and fucoidan (addition amounts 1.0, 1.5, 2.0, and 2.5 g / kg) was used as the test group. Individual feeds were designated at 10 o'clock daily, and the feeding rate of each group was set to 4% of the yellow tail weight. Experimental method: Various test feeds were continuously fed for 39 days. The fish were initially cultured for 14 days. After the initial breeding was completed, the yellow tail of each group was infected with Ildovirus on the day. The number of test days was 25 days to determine the survival rate of the yellow tail of each group. The infection is based on the following 4-TT ^ -r —____ _9_ The paper size is suitable for financial and family care standards (CNS) A4 specifications ('---- ^: Γid ^ n (Read the precautions on the back before filling in this page}

1T ΑΊ treatment i, description of the invention (method 7). The yellowtail fish infected with Ildovirus was homogenized in sterile seawater, and then centrifuged at a south speed. The resulting supernatant was diluted with seawater to 1/8, 000. The yellow tail of each group was placed in such a suspension and oxygenated for 2 hours while being soaked. The water temperature was maintained at 20 ° C during the test. As for the yellow tail that died during the test, whether it was infected with Ilido virus was confirmed by PCR. As a result, the yellow tail was found Dead: All due to infection with the virus. I The inventory after infection is shown in Figure 2. It was found that the yellow tail due to the disease of the mother can be prevented by fucoidan, and is industrially applied in a dose phase according to the present invention. Face Shao Dejiu Jiu __p,…, J, the staff can be confused and safe to prevent or treat infectious diseases, so it can stably supply farmed fish to the market. I ^ ^ 1.-, I- !! (Read the note on the back first Please fill in this page again j-Order • I. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Lithuania _ _-ιυ _

This paper size applies to China National Standard (CNS) Α4 gauge (210X 297 public IT

Claims (1)

513289 v on 8 8 A BCD No. 087104212 Patent Application Chinese Patent Application Amendment (October 91) 々 、 Application for Patent Scope 1. A fish agent for PRDV or Ildor (/ rzWoWr heart) virus infection A prophylactic and therapeutic agent for infectious diseases, comprising a sulfated polysaccharide as an active ingredient, the sulfated polysaccharide is selected from the group consisting of fucoidan, rhamnan sulfate, and carrageenan ( carrageenan). 2. The prophylactic and therapeutic agent for fish-borne infectious diseases, such as the item 1 of the patent application scope, wherein the sulfated polysaccharide is fucoidan. 3. For the prevention and treatment of fish-borne infectious diseases in the scope of patent application No. 1, it is used in the form of feed. The preventive and therapeutic agent for aquaculture fish media such as Tunu's patent application item 3, wherein the sulfated polysaccharide is fucoidan. This paper size applies to China National Standard (CNS) A4 (210X297 mm)