JP4331824B2 - Infectious disease prevention and treatment for seafood - Google Patents
Infectious disease prevention and treatment for seafood Download PDFInfo
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- JP4331824B2 JP4331824B2 JP14641199A JP14641199A JP4331824B2 JP 4331824 B2 JP4331824 B2 JP 4331824B2 JP 14641199 A JP14641199 A JP 14641199A JP 14641199 A JP14641199 A JP 14641199A JP 4331824 B2 JP4331824 B2 JP 4331824B2
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Description
【０００１】
【発明の属する技術分野】
本発明は、魚介類の病原性細菌及びウイルス等による感染症を予防・治療する薬剤、これを含有する飼料及びこれを投与することによる魚介類の養殖方法に関するものである。
【０００２】
【従来の技術】
クルマエビやブリ、マダイ、ヒラメ等の養殖場では、魚介類が高い密度で飼育されているため、病気が発生することが多い。病気の原因は、病原性細菌、ウイルス、寄生虫等による感染症が主であり、これに対し様々な対策が講じられている。
【０００３】
感染症の中でも、数年前から台湾や中国、東南アジアで急激に流行し始めたエビ類を死滅させるウイルス感染症（ＰＲＤＶ：Penaeid Rod-Shaped ＤＮＡ Virus）は、輸入業者によって日本にも伝播し、深刻な被害を発生させている。また、ブリのイリドウイルス感染症や連鎖球菌症等も発生頻度の高い感染症として重篤な被害をもたらしている。
【０００４】
これら感染症に対しては、抗生物質や免疫賦活物質等を投与するなどの対抗手段が講じられている。しかし、抗生物質を投与する場合、養魚等への残留が懸念され、またその効果は必ずしも満足しうるものではなかった。
【０００５】
一方、最近になって、硫酸化多糖類の魚類感染症に対する効果も知られるようになっている。例えば、特公平７−１０８８５８号公報には、各種海藻類の水抽出物がコイやブリ等の細菌感染症を予防することが記載されている。また、本出願人も硫酸化多糖類がウイルス感染の予防・治療効果を有することを見出し、これを特許出願している（ＷＯ９８／４２２０４号公報）。
更に、本出願人は、水溶性タンパク質又はペプチドを資化する能力を有するバチルス属細菌が、魚介類の養殖場等における硫化物の発生を抑制し、歩留まりを増加させることを見出し、特許出願している（ＷＯ９８／４５４０２号公報）。
【０００６】
【発明が解決しようとする課題】
上記のように魚介類の養殖場等における感染症を予防・治療する試みは多種なされているものの、未だ被害の発生は減少しておらず、より効果の高い新たな予防・治療剤が要望されている。
また、ウイルス感染又は細菌感染のどちらか一方に効果的な薬剤でも、他方を抑制できないものが多く、このことが養魚の歩留まり低下を防ぐことのできない原因となることが多い。このため、ウイルス及び細菌の両者の感染に対して優れた予防・治療効果を有するものが望まれている。
【０００７】
従って、本発明は、ウイルス感染及び細菌感染に対する優れた予防・治療効果を有するとともに、安価で安全性の高い魚介類の感染症予防・治療剤を提供することを目的としている。
【０００８】
【課題を解決するための手段】
本発明者らは鋭意研究したところ、フコイダンを多く含有するもずくには抗菌作用のあることが知られており（特開平１０−３０６０３５号公報）、フコイダンと細菌との併用は不可能と考えられたが、全く意外にも、フコイダンとある種のバチルス属細菌を併用して魚介類に投与することにより、相乗的に高い感染症予防・治療効果が得られ、歩留まりが増加することを見出し本発明を完成した。
【０００９】
すなわち、本発明は、フコイダン及びバチルス・パミルス又はバチルス・レンタスに属する細菌を含有することを特徴とする魚介類の感染症予防・治療剤を提供するものである。
また、本発明は、フコイダン及びバチルス・パミルス又はバチルス・レンタスに属する細菌を含有することを特徴とする魚介類の養殖用飼料を提供するものである。
更に、本発明は、フコイダン及びバチルス・パミルス又はバチルス・レンタスに属する細菌を投与することを特徴とする魚介類の養殖方法を提供するものである。
【００１０】
【発明の実施の形態】
本発明の第一の成分であるフコイダンは主としてフコースからなる分子量数十万前後の硫酸化多糖類であって、褐藻類のモズク、ウミウチワ、マコンブ、ワカメ、コンブ、アミジグサ等に豊富に含有されており人体に無害のものである。
フコイダンは、褐藻類を粉砕等して直接給餌してもよい。また、フコイダンは、抽出方法が確立されており、抽出物も好適に使用し得る。抽出方法としては、酸抽出法、熱水抽出法等が挙げられるが、これらに限定されず、種々の方法で抽出することができる。また、フコイダンは、海藻から抽出された高分子量のものを例えば弱酸処理等による加水分解等により、低分子化もしくはオリゴ糖化させてから用いてもよい。
【００１１】
一方、本発明の第二の成分であるバチルス・パミルス又はバチルス・レンタスとしては、特に、ＷＯ９８／４５４０２に開示されたバチルス・パミルスＡ−１株（ＦＥＲＭ ＢＰ−６２８０）、バチルス・レンタスＡ−２株（ＦＥＲＭ ＢＰ−６２７８）、バチルス・レンタスＡ−３株（ＦＥＲＭ ＢＰ−６２７９）、バチルス・パミルスＡ−４株（ＦＥＲＭ ＢＰ−６２８１）から選ばれる１種又は２種以上を用いることが好ましい。
【００１２】
これらのバチルス属細菌の投与形態としては、菌体を通常の増殖用培地、例えば、乾燥ブイヨン等で増殖させたものをそのまま投与してもよく、該培養液から遠心分離、静置して上清を除く等の手段により回収した菌体を投与してもよい。また、菌体は澱粉、スキムミルク等の賦形剤等とともに凍結乾燥したものを用いてもよい。更に、培養後の菌体を担体に担持させたものを投与してもよい。ここで用いる担体は特に限定されないが、ゼオライト、麦飯石、活性炭、火成岩、サンゴ石等が菌体の保持能の点で好ましく、特にゼオライトがコスト面から好ましい。
上記フコイダン及び前記バチルス属細菌は、両者を別個に投与することができる。すなわち、フコイダンをそのままあるいはこれを含む海藻類又は抽出乾燥物を直接給餌し、別途前記バチルス属細菌の培養液あるいは遠心分離等の手段により回収した菌体をそのまま、又は担体に担持して投与してもよい。
【００１３】
また、これらは通常魚介類の養殖に用いられる飼料原料に混合又は配合して用いてもよい。飼料に混合又は配合する両者の量は、魚介類や養殖方法により異なるが、フコイダンは一般的に感染予防のためには、０．５〜５０ｇ／kg程度が好ましい。一方、前記バチルス属細菌の量も魚介類の種類等に合わせ適宜決定すればよく、通常、１×１０4cfu／ｇ〜１×１０8cfu／ｇ程度、特に、１×１０5cfu／ｇ〜１×１０7cfu／ｇ程度が養魚の歩留まり、コスト面、作業性等の点から好ましい。これらを含有する飼料の給餌量は、１日に魚介類の体重の３％程度がよい。
【００１４】
両者を別個に投与する場合の投与量は、フコイダンは上記と同様となるよう設定すればよい。また、前記バチルス属細菌は１×１０5cfu／m3〜１×１０9cfu／m3程度、特に、１×１０6cfu／m3〜１×１０8cfu／m3程度養殖場等へ投与することが好ましい。
【００１５】
本発明の感染症予防・治療剤を適用し得る魚介類としては、特に限定されず、淡水魚として、ウナギ、コイ、ニジマス、アユ、ティラピア、フナ等、海水魚として、ブリ、タイ、ギンザケ、マアジ、ヒラメ、カレイ、クロソイ、トラフグ、カンパチ等、無脊椎動物としてクルマエビ、ブラックタイガー、ウシエビ、コウライエビ、ガザミ等が挙げられる。また、適用できる感染症としては、イリドウイルス、ビルナウイルス、ＰＲＤＶ等のウイルス感染症、ビブリオ、シュードモナス、連鎖球菌、類結節症等の細菌感染症が挙げられる。中でも、イリドウイルスやＰＲＤＶ、連鎖球菌症に対する効果が高く好ましい。
【００１６】
【実施例】
以下、実施例を挙げて本発明を更に詳細に説明するが、本発明はこれらに限定されるものではない。
【００１７】
実施例１
ブリの連鎖球菌症とイリドウイルス症の混合感染に対するフコイダン及びバチルス・パミルスの併用効果を検討した。
（供試魚）鹿児島県下のブリ養殖場において、網生簀に養殖中の平均体重２５０ｇ／尾のブリ群４生簀を試験に供した。
（飼育方法）１０ｍ×１０ｍ×１５ｍの網生簀４基を使用した。
（供試尾数）試験には自然発症した連鎖球菌症とイリドウイルス症の混合感染を確認したブリ群４０，０００尾（各試験区１０，０００尾）を供した。
（飼料）対照区には冷凍マイワシ８０部とブリ用モイスト粉末（ミヤコ化学製）２０部を混合し、成形したブリ用モイストペレット（対照飼料）を使用した。試験区１（フコイダン・バチルス併用投与区）には冷凍マイワシ８０部とブリ用モイスト粉末（同上）２０部に加えて、ブリ体重１kgあたりフコイダンを６０mg及び飼料１ｇあたりバチルス・パミルスを１０6個となるように添加混合し、成形したブリ用モイストペレット（試験飼料１）を、試験区２（フコイダン単用投与区）には同様にフコイダンを６０mgとなるように、試験区３（バチルス単用投与区）には同様にバチルス・パミルスを１０6個となるように添加混合し、成形したブリ用モイストペレット（試験飼料３）を使用した。
給餌は毎日定刻の１０時に行い、設定給餌率はブリの総体重量あたり８％とした。
【００１８】
（試験方法）試験期間を８月３日から８月２２日の２０日間と定め、ブリの日間斃死数を求めた。
試験期間中に斃死したブリを任意に採取し、細菌検査によって連鎖球菌感染、ＰＣＲ法によってイリドウイルス感染の有無を確認した。その結果、斃死の原因は連鎖球菌症とイリドウイルス症の混合感染によるものであった。
【００１９】
試験期間中の日間斃死数は図１に示す。その結果、フコイダン及びバチルス・パミルスの併用投与により、それぞれの単独投与に比べて、ブリの連鎖球菌症とイリドウイルス症の混合感染による死亡を顕著に防止できた。
【００２０】
実施例２
ブリの連鎖球菌症とイリドウイルス症の混合感染に対するフコイダン及びバチルス・パミルスの併用効果を検討した。
（供試魚）鹿児島県下のブリ養殖場において、網生簀に養殖中の平均体重４５０ｇ／尾のブリ群６生簀を試験に供した。
（飼育方法）１０ｍ×１０ｍ×１５ｍの網生簀６基を使用した。
（供試尾数）試験には自然発症した連鎖球菌症とイリドウイルス症の混合感染を確認したブリ群３０，０００尾（各試験区５，０００尾）を供した。
（飼料）対照区には冷凍マイワシ８０部とブリ用モイスト粉末（同上）２０部を混合し、形成したブリ用モイストペレット（対照飼料）を使用した。試験区１（フコイダン・バチルス併用投与区）には冷凍マイワシ８０部とブリ用モイスト粉末（同上）２０部に加えて、ブリ体重１kgあたりフコイダンを６０mg及び飼料１ｇあたりバチルス・パミルスを１０6個となるように添加混合し、成形したブリ用モイストペレット（試験飼料１）を、試験区２（フコイダン単用投与区１）には同様にフコイダンを６０mg、試験区３（フコイダン単用投与区２）には同様にフコイダンを１００mgとなるように添加混合し、成形したブリ用モイストペレット（試験飼料２、３）を、試験区４（バチルス単用投与区１）には同様にバチルス・パミルスを１０6個、試験区５（バチルス単用投与区２）には同様にバチルス・パミルスを１０8個となるように添加混合し、成形したブリ用モイストペレット（試験飼料４、５）を使用した。
（給餌方法）給餌は毎日定刻の１０時に行った。設定給餌率はブリの総体重あたり６％とした。試験開始時に、対照区には対照飼料１３５kgを、各試験区には各試験飼料１３５kgを給餌し、成長及び生存尾数をみて適宜給餌量を増減した。
（試験方法）試験期間を９月１日から９月１４日の１４日間と定め、ブリの日間斃死数を求めた。
試験期間中に斃死したブリを任意に採取し、細菌検査によって連鎖球菌感染、ＰＣＲ法によってイリドウイルス感染の有無を確認した。その結果、斃死の原因は連鎖球菌症とイリドウイルス症の混合感染によるものであった。
【００２１】
試験期間中の日間斃死数を図２に示した。その結果、フコイダン及びバチルス・パミルスの併用投与により、それぞれの単独投与に比べて、ブリの連鎖球菌症とイリドウイルス症の混合感染による死亡をより顕著に防止できた。
また、フコイダン投与量を６０mg／kgから１００mg／kgに増加させても、またバチルス・パミルスを飼料１ｇあたり１０6個から１０8個に増加させてもその感染防止効果に差がなかったことから、本発明のフコイダンとバチルス・パミルスとの併用によって得られる効果は、単なる相加効果でなく相乗効果であることが明らかである。
【００２２】
【発明の効果】
本発明によれば、魚介類のウイルスや細菌による感染症、特に両者の混合感染症に対して予防することができる。魚介類の養殖時の飼料として用いてもよい。
【図面の簡単な説明】
【図１】連鎖球菌とイリドウイルス症の混合感染ブリに対するフコイダン及びバチルス・パミルスの併用効果を示す図である。
【図２】連鎖球菌とイリドウイルス症の混合感染ブリに対するフコイダン及びバチルス・パミルスの併用効果を示す図である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a drug for preventing and treating infections caused by pathogenic bacteria and viruses of fish and shellfish, a feed containing the same, and a method for culturing fish and shellfish by administering the same.
[0002]
[Prior art]
In farms such as prawns, yellowtails, red sea breams, and Japanese flounder, diseases often occur because of the high density of seafood. Causes of diseases are mainly infections caused by pathogenic bacteria, viruses, parasites, etc., and various countermeasures have been taken against them.
[0003]
Among infectious diseases, viral infections (PRDV: Penaeid Rod-Shaped DNA Virus) that kills shrimp that began to spread rapidly in Taiwan, China, and Southeast Asia for several years have been transmitted to Japan by importers. Serious damage has occurred. In addition, yellowtail iridovirus infection, streptococcal disease, and the like have caused serious damage as frequently occurring infections.
[0004]
Countermeasures such as administration of antibiotics or immunostimulatory substances have been taken against these infectious diseases. However, in the case of administering antibiotics, there is concern about remaining in fish farms, etc., and the effect is not always satisfactory.
[0005]
On the other hand, recently, the effect of sulfated polysaccharides on fish infections is also known. For example, Japanese Patent Publication No. 7-108858 discloses that water extracts of various seaweeds prevent bacterial infections such as carp and yellowtail. The present applicant has also found that sulfated polysaccharides have the effect of preventing and treating viral infection, and has filed a patent application for this (WO 98/42204).
Furthermore, the present applicant has found that Bacillus bacteria having the ability to assimilate water-soluble proteins or peptides suppress the generation of sulfides in fish farms and the like, and increase the yield, and file a patent application. (WO98 / 45402).
[0006]
[Problems to be solved by the invention]
Although various attempts have been made to prevent and treat infectious diseases in seafood farms, as described above, the occurrence of damage has not been reduced, and there has been a demand for new, more effective preventive and therapeutic agents. ing.
In addition, there are many drugs that are effective against either viral infection or bacterial infection, and the other cannot be suppressed, and this often causes a failure to prevent a decrease in the yield of fish farming. For this reason, what has the outstanding prevention and treatment effect with respect to the infection of both a virus and bacteria is desired.
[0007]
Accordingly, an object of the present invention is to provide an inexpensive prophylactic / therapeutic agent for fish and shellfish infections that has an excellent preventive / therapeutic effect on viral infection and bacterial infection.
[0008]
[Means for Solving the Problems]
As a result of diligent research, the present inventors have known that mozuku containing a large amount of fucoidan has an antibacterial action (Japanese Patent Laid-Open No. 10-306035), and it is considered impossible to use fucoidan in combination with bacteria. However, surprisingly, it has been found that the combined use of fucoidan and certain Bacillus bacteria in fish and shellfish results in synergistic high infection prevention and treatment effects and increases the yield. Completed the invention.
[0009]
That is, the present invention provides a prophylactic / therapeutic agent for fish and shellfish infections characterized by containing a bacterium belonging to Fucoidan and Bacillus pamilus or Bacillus lentus.
The present invention also provides a fish and shellfish aquaculture feed comprising fucoidan and bacteria belonging to Bacillus pamilus or Bacillus lentus.
Furthermore, the present invention provides a method for cultivating seafood characterized by administering fucoidan and bacteria belonging to Bacillus pamilus or Bacillus lentus.
[0010]
DETAILED DESCRIPTION OF THE INVENTION
Fucoidan, the first component of the present invention, is a sulfated polysaccharide mainly composed of fucose having a molecular weight of around several hundred thousand, and is abundantly contained in brown algae mozuku, sea urchin, macabu, wakame, kombu, ajigusa, etc. It is harmless to the human body.
Fucoidan may be fed directly by pulverizing brown algae. Further, fucoidan has an established extraction method, and an extract can also be preferably used. Examples of the extraction method include an acid extraction method and a hot water extraction method, but the extraction method is not limited to these, and extraction can be performed by various methods. Further, fucoidan may be used after a high molecular weight substance extracted from seaweed is reduced in molecular weight or oligosaccharide by, for example, hydrolysis by weak acid treatment or the like.
[0011]
On the other hand, as Bacillus pamilus or Bacillus lentus which is the second component of the present invention, in particular, Bacillus pamilus A-1 strain (FERM BP-6280), Bacillus lentus A-2 disclosed in WO98 / 45402. It is preferable to use one or more selected from a strain (FERM BP-6278), a Bacillus lentus A-3 strain (FERM BP-6279), and a Bacillus pamilus A-4 strain (FERM BP-6281).
[0012]
As for the administration form of these Bacillus bacteria, those obtained by growing the cells in a normal growth medium, for example, dry broth, may be administered as it is, and the culture solution is centrifuged and left standing. The cells recovered by means such as removing the liquid may be administered. The cells may be lyophilized with excipients such as starch and skim milk. Furthermore, you may administer what supported the microbial cell after culture | cultivation on the support | carrier. The carrier used here is not particularly limited, but zeolite, barley stone, activated carbon, igneous rock, coral stone and the like are preferable from the viewpoint of the ability to retain cells, and zeolite is particularly preferable from the viewpoint of cost.
Both the fucoidan and the Bacillus bacterium can be administered separately. That is, fucoidan is directly fed as it is or seaweed containing it or an extracted dried product, and the cells recovered separately by means of a culture solution or centrifugation of the genus Bacillus are administered as they are or carried on a carrier. May be.
[0013]
Moreover, you may mix and mix | blend these with the feed raw material normally used for the culture of seafood. The amount of both to be mixed or blended in the feed varies depending on the seafood and the cultivation method, but fucoidan is generally preferably about 0.5 to 50 g / kg for preventing infection. On the other hand, the amount of the bacterium belonging to the genus Bacillus may be appropriately determined according to the type of fish and shellfish, etc., and is usually about 1 × 10 4 cfu / g to 1 × 10 8 cfu / g, particularly 1 × 10 5 cfu / g. About 1 × 10 7 cfu / g is preferable from the viewpoint of fish yield, cost, workability, and the like. The feed amount of feed containing these is preferably about 3% of the weight of fish and shellfish per day.
[0014]
What is necessary is just to set the dosage in the case of administering both separately so that fucoidan may become the same as the above. The Bacillus bacterium is about 1 × 10 5 cfu / m 3 to 1 × 10 9 cfu / m 3 , especially about 1 × 10 6 cfu / m 3 to 1 × 10 8 cfu / m 3 Administration is preferred.
[0015]
The seafood to which the infectious disease preventive / therapeutic agent of the present invention can be applied is not particularly limited, and as freshwater fish, eel, carp, rainbow trout, sweetfish, tilapia, crucianfish, etc., as saltwater fish, yellowtail, Thai, coho salmon, horse mackerel , Flounder, flatfish, blackfish, tiger puffer, amberjack, etc. Examples of invertebrates include prawns, black tigers, tiger prawns, tiger prawns, crab and the like. Infectious diseases that can be applied include viral infections such as iridovirus, birnavirus, and PRDV, and bacterial infections such as vibrio, pseudomonas, streptococci, and nodules. Especially, the effect with respect to an iridovirus, PRDV, and streptococci is high and preferable.
[0016]
【Example】
EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not limited to these.
[0017]
Example 1
We investigated the combined effect of fucoidan and Bacillus pamilus on mixed infections of yellow streptococci and iridovirosis.
(Test fish) At a yellowtail farm in Kagoshima Prefecture, four yellowtails with an average weight of 250 g / tail were cultured for the net cage.
(Raising method) Four 10m × 10m × 15m net ginger were used.
(Number of test specimens) For the test, 40,000 fish (10,000 test animals in each test group) were confirmed, which confirmed mixed infection of spontaneous streptococcal disease and iridovirosis.
(Feed) In the control group, 80 parts of frozen sardine and 20 parts of moist powder for brie (Miyako Chemical Co., Ltd.) were mixed, and the molded moist pellets for brie (control feed) were used. In Test Zone 1 (Fucoidan / Bacillus combined administration zone), in addition to 80 parts of frozen sardine and 20 parts of Moist powder for yellowtail (same as above), 60 mg of Fucoidan per kg of yellowtail body weight and 10 6 Bacillus pamilus per gram of feed Add and mix so as to form the bristed moist pellets (test feed 1), and in test group 2 (fucoidan single administration group) similarly, test group 3 (bacillus single administration) so that fucoidan is 60 mg. In the same manner, the Boiled moist pellets (test feed 3) formed by adding and mixing 10 6 Bacillus pamils in the same manner were used.
Feeding was performed every day at 10 o'clock, and the set feeding rate was 8% per total weight of yellowtail.
[0018]
(Test Method) The test period was set to 20 days from August 3 to August 22, and the number of daily deaths of yellowtail was determined.
The yellowtail drowned during the test period was arbitrarily collected, and the presence of streptococcal infection was confirmed by a bacterial test and the presence of an iridovirus infection was confirmed by a PCR method. As a result, the cause of drowning was due to a mixed infection of streptococci and iridovirosis.
[0019]
The number of daily deaths during the test period is shown in FIG. As a result, the combined administration of fucoidan and Bacillus pamilus significantly prevented death due to mixed infection of streptococcal disease and iridovirosis in yellowtail compared to each single administration.
[0020]
Example 2
We investigated the combined effect of fucoidan and Bacillus pamilus on mixed infections of yellow streptococci and iridovirosis.
(Test fish) At a yellowtail farm in Kagoshima prefecture, 6 yellowtails with an average weight of 450 g / tail and a tail group were subjected to the test.
(Raising method) 6 net ginger cocoons of 10 m × 10 m × 15 m were used.
(Number of test specimens) For the test, 30,000 fish (5,000 fish in each test group) were confirmed, which confirmed mixed infection of spontaneous streptococci and iridovirosis.
(Feed) In the control group, 80 parts of frozen sardine and 20 parts of the moist powder for yellowtail (same as above) were mixed, and the formed yellowish moist pellets (control feed) were used. In Test Zone 1 (Fucoidan / Bacillus combined administration zone), in addition to 80 parts of frozen sardine and 20 parts of Moist powder for yellowtail (same as above), 60 mg of Fucoidan per kg of yellowtail body weight and 10 6 Bacillus pamilus per gram of feed Moist pellets for yellowtail (test feed 1) that were added and mixed in the same manner as above, 60 mg of fucoidan were similarly used in test group 2 (fucoidan single administration group 1), and test group 3 (fucoidan single administration group 2) In the same way, fucoidan was added and mixed so as to be 100 mg, and the molded bristed moist pellets (test feeds 2 and 3) were similarly used. In test group 4 (bacillus single-use administration group 1), 10 6, test group 5 (the Bacillus single administration group 2) was added and mixed so as to be 10 8 Bacillus Pamirusu Similarly, molded Buri for moist pellet (test shepherd 4, 5) was used.
(Feeding method) Feeding was performed every day at 10 o'clock. The set feeding rate was 6% per total weight of yellowtail. At the start of the test, the control group was fed with 135 kg of the control feed, and each test group was fed with 135 kg of each test feed.
(Test method) The test period was determined to be 14 days from September 1 to September 14, and the number of daily deaths of yellowtail was determined.
The yellowtail drowned during the test period was arbitrarily collected, and the presence of streptococcal infection was confirmed by a bacterial test and the presence of an iridovirus infection was confirmed by a PCR method. As a result, the cause of drowning was due to a mixed infection of streptococci and iridovirosis.
[0021]
The number of daily deaths during the test period is shown in FIG. As a result, the combined administration of fucoidan and Bacillus pamilus more significantly prevented death due to mixed infection of streptococcal disease and iridovirosis of yellowtail compared to each single administration.
In addition, there was no difference in the infection prevention effect even when the fucoidan dose was increased from 60 mg / kg to 100 mg / kg and Bacillus pamilus was increased from 10 6 to 10 8 per gram of feed. It is clear that the effect obtained by the combined use of fucoidan and Bacillus pamils of the present invention is not a simple additive effect but a synergistic effect.
[0022]
【The invention's effect】
According to the present invention, it is possible to prevent infections caused by viruses and bacteria of fish and shellfish, particularly mixed infections of both. You may use as a feed at the time of cultivation of seafood.
[Brief description of the drawings]
FIG. 1 is a graph showing the combined effect of fucoidan and Bacillus pamilus on a mixed yellowtail of streptococci and iridovirosis.
FIG. 2 is a graph showing the combined effect of fucoidan and Bacillus pamilus against mixed yellowtail of streptococci and iridovirosis.
Claims (3)
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| Application Number | Priority Date | Filing Date | Title |
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| JP14641199A JP4331824B2 (en) | 1999-05-26 | 1999-05-26 | Infectious disease prevention and treatment for seafood |
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| JP14641199A JP4331824B2 (en) | 1999-05-26 | 1999-05-26 | Infectious disease prevention and treatment for seafood |
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| JP2000336035A JP2000336035A (en) | 2000-12-05 |
| JP4331824B2 true JP4331824B2 (en) | 2009-09-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110679518A (en) * | 2019-09-30 | 2020-01-14 | 周怀林 | Method for breeding tilapia with crisp meat |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1523995A (en) * | 2001-05-11 | 2004-08-25 | 株式会社益力多本社 | Preventives and/or remedies for fish infections |
| JP4398172B2 (en) * | 2003-04-02 | 2010-01-13 | 株式会社ヤクルト本社 | Infection prevention and treatment agent for fish |
| WO2007077606A1 (en) * | 2005-12-28 | 2007-07-12 | Ahc Co., Ltd | Efficacy enhancer for alternative therapeutic agent and anticancer preparation making use of the same |
| FR2998176B1 (en) | 2012-11-16 | 2015-01-16 | Univ Blaise Pascal Clermont Ii | POLYSACCHARIDE SULFATE COMPOSITION |
| CN103960524A (en) * | 2014-04-11 | 2014-08-06 | 汕头大学 | Tilapia mossambica feed and preparation method thereof |
| CN106676029B (en) * | 2016-05-03 | 2021-06-11 | 天津昌农科技有限责任公司 | Bacillus pumilus strain, and microecological preparation and feed thereof |
| CN108812462A (en) * | 2018-06-26 | 2018-11-16 | 徐州市若水锦鲤养殖有限公司 | A kind of control method of fishpond insect pest |
| CN111109160B (en) * | 2020-01-13 | 2023-04-25 | 广西壮族自治区水产引育种中心 | Method for breeding good carp variety by hybridization |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110679518A (en) * | 2019-09-30 | 2020-01-14 | 周怀林 | Method for breeding tilapia with crisp meat |
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