WO1997007784A2 - LIPOSOMAL PHOSPHODIESTER, PHOSPHOROTHIOATE, AND p-ETHOXY OLIGONUCLEOTIDES - Google Patents

LIPOSOMAL PHOSPHODIESTER, PHOSPHOROTHIOATE, AND p-ETHOXY OLIGONUCLEOTIDES Download PDF

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Publication number
WO1997007784A2
WO1997007784A2 PCT/US1996/014146 US9614146W WO9707784A2 WO 1997007784 A2 WO1997007784 A2 WO 1997007784A2 US 9614146 W US9614146 W US 9614146W WO 9707784 A2 WO9707784 A2 WO 9707784A2
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WIPO (PCT)
Prior art keywords
oligonucleotides
oligonucleotide
oligos
composition
liposomal
Prior art date
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Ceased
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PCT/US1996/014146
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English (en)
French (fr)
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WO1997007784A3 (en
Inventor
Gabriel Lopez-Berestein
Ana Maria Tari
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University of Texas System
University of Texas at Austin
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University of Texas System
University of Texas at Austin
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Application filed by University of Texas System, University of Texas at Austin filed Critical University of Texas System
Priority to AU69129/96A priority Critical patent/AU6912996A/en
Priority to AT96929888T priority patent/ATE253897T1/de
Priority to EP96929888A priority patent/EP0847272B1/en
Priority to CA2228944A priority patent/CA2228944C/en
Priority to JP51064297A priority patent/JP4291412B2/ja
Priority to DE69630691T priority patent/DE69630691T2/de
Priority to DK96929888T priority patent/DK0847272T3/da
Publication of WO1997007784A2 publication Critical patent/WO1997007784A2/en
Publication of WO1997007784A3 publication Critical patent/WO1997007784A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/312—Phosphonates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates

Definitions

  • the present invention relates to liposomal formulations of certain antisense oligonucleotides, specifically liposomal phosphodiester, phosphorothioate, and p- ethoxy oligonucleotides.
  • the invention also relates to methods of making such formulations and methods of using such formulations in medical therapy.
  • Antisense oligonucleotides complementary to specific regions of the target mRNA, have been used to inhibit the expression of endogenous genes. When the antisense oligonucleotides bind to the target mRNA, a DNA-RNA hybrid is formed. This hybrid formation inhibits the translation of the mRNA and, thus, the gene's expression ofthe protein. Ifthe protein is essential for the survival of the cell, the inhibition of its expression may lead to cell death. Therefore, antisense oligonucleotides can be useful tools in anticancer and antiviral therapies.
  • the main obstacles in using antisense oligonucleotides to inhibit gene expression are cellular instability, low cellular uptake, and poor intracellular delivery.
  • Natural phosphodiesters are not resistant to nuclease hydrolysis; thus high concentrations of antisense oligonucleotides are needed before any inhibitory effect is observed.
  • Modified phosphodiester analogs, such as phosphorothioates have been made to overcome this nuclease hydrolysis problem, but they have not provided a completely satisfactory solution to the problem.
  • the cellular uptake of antisense oligonucleotides is low.
  • physical techniques such as calcium-phosphate precipitation, DEAE-dextran mediation, or electroporation have been used to increase the cellular uptake of oligonucleotides. These techniques are difficult to reproduce and are inapplicable in vivo.
  • Cationic lipids such as Lipofectin, have also been used to deliver phosphodiester or phosphorothioate oligonucleotides. An electrostatic interaction is formed between the cationic lipids and the negatively charged phosphodiester or phosphorothioate oligonucleotides, which results in a complex that is then taken up by the target cells.
  • these cationic lipids do not protect the oligonucleotides from nuclease digestion, they are only useful in delivering the nuclease-resistant phosphorothioates, but not the nuclease-cleavable phosphodiesters.
  • pE oligos Another modified phosphodiester (PD) analog that has been prepared is p- ethoxy (pE) oligos.
  • the modifications of pE oligos are made in the phosphate backbone so that the modification will not interfere with the binding of these oligos to the target mRNA.
  • pE oligos are made by adding an ethyl group to the nonbridging oxygen atom of the phosphate backbone, thus rendering these oligos uncharged compounds.
  • One embodiment of the present invention is a liposomal composition of antisense oligonucleotides.
  • the composition includes (a) a liposome which consists essentially of neutral phospholipids, and (b) an antisense oligonucleotide that is - D -
  • phosphodiester oligonucleotides phosphorothioate oligonucleotides, and p-ethoxy oligonucleotides.
  • the phospholipids are preferably phosphatidylcholines.
  • An especially preferred phospholipid is dioleoylphosphatidyl choline.
  • the antisense oligonucleotide is a phosphodiester oligonucleotide
  • the preferred molar ratio of phospholipid to oligo is less than about 3,000:1.
  • the antisense oligonucleotide is a phosphorothioate oligonucleotide
  • the preferred molar ratio of phospholipid to oligo is between about 10:1 and about 50:1.
  • the antisense oligonucleotide is a p-ethoxy oligonucleotide
  • the preferred molar ratio of phospholipid to oligo is between about 5:1 and about 100:1.
  • Another embodiment of the present invention is a method of inhibiting the growth of tumor cells in mammals.
  • the method comprises the step of administering to a mammalian subject having a tumor an amount of the above-described composition that is effective to inhibit the growth of tumor cells.
  • Another embodiment of the present invention is a method of preparing the liposomal composition of antisense oligonucleotides.
  • the method comprises the steps of (a) hydrating a lyophilized composition that consists essentially of neutral phospholipids and an antisense oligonucleotide that is selected from the group consisting of phosphodiester oligonucleotides, phosphorothioate oligonucleotides, and p-ethoxy oligonucleotides, thereby forming an aqueous suspension which includes free oligonucleotide and liposomes entrapping oligonucleotide; and (b) separating the free oligonucleotide from the liposomes by dialysis.
  • the aqueous suspension is sonicated before dialysis.
  • compositions of the present invention constitute an improved delivery system for antisense oligos, such as those used in anti-cancer therapy.
  • the liposomal compositions of the present invention result in increased cellular uptake and intracellular delivery of the antisense oligos, as compared to prior art compositions. Therefore, when such compositions are used to deliver oligos that inhibit the expression of a gene found in cancerous cells but not in normal cells, the therapeutic results are enhanced.
  • Cancers which may be treated in accordance with the present invention would be numerous, with leukemias being one prominent example.
  • the formulations of the present invention also enhance the incorporation of oligos in the liposomes, as compared to prior art liposomal formulations.
  • Figures 1 A and IB are photographs showing the uptake of (A) free and (B) liposomal pE oligos by ALL-1 cells.
  • Figure 2A shows the extent of growth inhibition that resulted when ALL-1 and HL60 cells were incubated with liposomal-pE antisense oligos targeted against the B1/A2 breakpoint junction of Bcr-Abl mRNA.
  • Figure 2B shows the extent of growth inhibition that resulted when ALL-1 cells were incubated with B1/A2 liposomal -pE antisense oligos and B2/A2 liposomal- pE control oligos.
  • Figure 3 A shows the extent of growth inhibition that resulted when BVI 73 and HL60 cells were incubated with liposomal-pE antisense oligos targeting against the B2/A2 breakpoint junction of Bcr-Abl mRNA.
  • Figure 3B shows the extent of growth inhibition that resulted when BVI 73 cells were incubated with liposomal-pE antisense oligos targeting against the B2/A2 breakpoint junction and with control oligos targeting against the B1/A2 breakpoint junction.
  • Figure 4A shows the extent of growth inhibition that resulted when K562 and HL60 cells were incubated with liposomal-pE antisense oligos targeting against the B3/A2 breakpoint junction of Bcr-Abl mRNA.
  • Figure 4B shows the extent of growth inhibition that resulted when K562 cells were incubated with liposomal-pE antisense oligos targeting against the B3/A2 breakpoint junction and with control oligos targeting against the B1/A2 breakpoint junction.
  • Figure 5 shows the extent of growth inhibition that resulted when Tween 20- containing liposomal-pE oligos were incubated with BVI 73 cells.
  • Liposomes is used in this patent to mean lipid-containing vesicles having a lipid bilayer, as well as other lipid carrier particles which can entrap antisense oligonucleotides.
  • the liposomes can be made of one or more phospholipids, as long as the lipid material is substantially uncharged. It is important that the composition be substantially free of anionic and cationic phospholipids and cholesterol. Suitable phospholipids include phosphatidyl cholines and others that are well known to persons that are skilled in this field.
  • the liposomes can be, for example, unilamellar, multilamellar, or have an undefined lamellar structure.
  • a pharmaceutical composition comprising the liposomes will usually include a sterile, pharmaceutically acceptable carrier or diluent, such as water or saline solution.
  • Entrap Entrap
  • encapsulate and “incorporate” are used in this patent to mean that the oligo is enclosed within at least some portion of the inner aqueous space (including the interlamellar regions ofthe bilayer) ofthe liposome.
  • a composition of the present invention is preferably administered to a patient parenterally, for example by intravenous, intraarterial, intramuscular, intralymphatic, intraperitoneal, subcutaneous, intrapleural, or intrathecal injection, or may be used in ex vivo bone marrow purging.
  • Preferred dosages are between 5-25 mg/kg.
  • the administration is preferably repeated on a timed schedule until the cancer disappears or regresses, and may be in conjunction with other forms of therapy.
  • the making and use of the present invention is further illustrated by the following examples.
  • Phospholipids were purchased from Avanti Polar Lipids.
  • Phosphodiesters were labeled at 37°C for 8 h with [ 32 P ⁇ ]ATP at the 5' end by T4 kinase.
  • the labeled oligonucleotide was precipitated with ethanol at -20°C overnight. After washing with 70% ethanol three times, phosphodiester oligonucleotides were twice filtered with a Microcon-3 filter to separate the labeled oligonucleotides from free [ 32 P ⁇ ]ATP.
  • Phosphorothioates labeled with 35 S, were provided by Genta Incorporated.
  • Liposomal-phosphodiester Preparation Phosphodiester oligonucleotides dissolved in distilled water were mixed with phospholipids in the presence of excess t-butanol so that the final volume of t-butanol in the mixture was 80-90%. Trace amounts of [ 3 H]cholestanyl ether and [ 32 P]phosphodiester were also added to the mixture as lipid and oligonucleotide markers, respectively. The mixture was vortexed before being frozen in an acetone/dry ice bath. The frozen mixture was lyophilized and hydrated with hepes buffered saline (1 mM Hepes and lOmM NaCl) overnight. Liposomes were twice sonicated for 10 min in a bath type sonicator.
  • Liposomal-phosphorothioates were prepared similarly to that described for liposomal-phosphodiesters, except phosphorothioates, instead of phosphodiesters, were used. Also, [ Sjphosphorothioates, instead of [ P]phosphodiesters, were used as the oligonucleotide marker. Separation of Free Oligonucleotides from those Incorporated in Liposomes
  • Dioleoylphosphatidylcholine (DOPC) lipids were chosen for the phosphodiester (PD) incorporation because they are neutral while PD is negatively charged. By using this lipid, electrostatic repulsion (which can lower incorporation) may be reduced. Positively charged lipids were not used because they may induce non-specific cellular toxicity.
  • the initial attempts of incorporating PD oligonucleotides into liposomes were done by using freeze-and-thaw, and dehydration-rehydration methods .
  • [ 3 H]labeled DOPC lipids were evaporated from their organic solvent under nitrogen gas to form a lipid film. After vacuum desiccation, the lipid film was hydrated with hepes buffered saline (1 mM Hepes, 10 mM NaCl, pH 8.0) and sonicated in a bath type sonicator. These pre-formed liposomes were then mixed with [ 32 P]labeled PD oligonucleotides at a 100 or 1000 to 1 molar ratio. The whole mixture was frozen in an acetone/dry ice bath for 5-10 minutes and thawed at room temperature for 25-30 minutes.
  • the mixture was loaded on a BioGel A0.5M column for separation of free PD oligonucleotides from liposomal-PD. Aliquots were taken before and after column loading and were sent to liquid scintillation counting. The incorporation was ⁇ 5% (Table 1).
  • PD dissolved in distilled water was mixed with DOPC in the presence of excess t-butanol so that the final volume of t-butanol in the mixture was 80-90% before being frozen in an acetone/dry ice bath and lyophilized. This was to avoid the step of preparing pre-formed liposomes. With this procedure, similar level of inco ⁇ oration (16.2%) was obtained at the 2000/1 molar ratio.
  • pE oligos were purchased from Oligos Therapeutics (Willsonville, OR). Phospholipids were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL).
  • Oligo labeling pE oligos were labeled at 37°C for 24 h with [ 32 P ⁇ ] ATP at the 5 ' end by T4 polynucleotide kinase, and then precipitated with ethanol at -20°C overnight. They were then washed with 70% ethanol three times to separate the labeled oligo from free [ 32 P ⁇ ]ATP.
  • the lipid phosphatidylcholine (PC) was chosen for the inco ⁇ oration of pE oligos because both PC and pE oligos are neutral molecules so they should be compatible.
  • PCS dioleoyl PC
  • DOPC dioleoyl PC
  • pE oligos Trace amounts of radio labeled pE oligos and DOPC were included in the mixture.
  • the DOPC/pE oligos mixtures were frozen in a dry ice/acetone bath before being lyophilized.
  • the lyophilized DOPC/pE oligo powder was then hydrated with Hepes buffered saline so that the final oligo concentration was 10 ⁇ M.
  • pE oligos were successfully inco ⁇ orated into DOPC liposomes, ranging between 28 to 83% efficiency (Table 5). The inco ⁇ oration efficiency was dependent on the molar ratios of DOPC to pE oligos: 10 > 100 > 5 > 1000:1.
  • ALL-1 cells which are human acute lymphocytic leukemic cells, were used. Fifty thousand ALL-1 cells/well were plated in a 24-well plate in 0.3 mL of medium. After 2 h of plating, final concentrations of 16 ⁇ M of liposomal or free pE oligos conjugated with rhodamine were added to ALL-1 cells. After 24 h of incubation, the cells were thrice washed with phosphate buffered saline before being viewed under a confocal laser scanning microscope. See Figure 1 (Uptake of (A) free or (B) liposomal-pE oligos by ALL-1 cells).
  • ALL-1 acute lymphocytic leukemia
  • BV173 chronic myelogenous leukemia
  • K562 chronic myelogenous leukemia
  • All three cell lines contain the rearranged Philadelphia (Ph) chromosome which arises from a reciprocal translocation of chromosomes 9 and 22. This translocation results in the relocation of the c-Abl protooncogene from chromosome 9 onto the 3 ' end of the breakpoint cluster region (Ber) of chromosome 22, thus producing a hybrid Bcr-Abl gene.
  • the breakpoint junctions where the Ber and the Abl genes fuse are different in the three cell lines.
  • ALL-1 cells the breakpoint junction is Ber exon 1/ Abl exon 2.
  • BV173 cells the breakpoint junction is Ber exon 2/ Abl exon 2.
  • K562 cells the breakpoint junction is Ber exon 3/ Abl exon 2. All these hybrid genes produce a novel Bcr- Abl fusion protein, which has enhanced tyrosine kinase activity that has been linked to the pathogenesis of the leukemias. Thus, inhibition of the production of the Bcr- Abl protein may lead to leukemic cell growth inhibition and even cell death.
  • the difference in absorbance between 570 and 595 nm will be taken as the overall absorbance value of the leukemic cells.
  • the viabilities of leukemic cells treated with liposomal-pE oligos will be compared with those of the control untreated cells.
  • the Ph chromosome-positive cell lines were incubated with antisense and control liposomal-pE oligos.
  • ALL-1 cells were incubated with the B1/A2 liposomal- pE antisense oligos and the B2/A2 liposomal-pE control oligos, growth inhibition was induced (Fig. 2B).
  • the B1/A2 antisense oligos induced a much greater inhibitory effect.
  • higher inhibitory effects on BV173 and K562 cells were found with the corresponding liposomal-pE antisense oligos than with the control oligos (Fig. 3B, 4B).
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PCT/US1996/014146 1995-08-29 1996-08-26 LIPOSOMAL PHOSPHODIESTER, PHOSPHOROTHIOATE, AND p-ETHOXY OLIGONUCLEOTIDES Ceased WO1997007784A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU69129/96A AU6912996A (en) 1995-08-29 1996-08-26 Liposomal phosphodiester, phosphorothioate, and p-ethoxy oligonucleotides
AT96929888T ATE253897T1 (de) 1995-08-29 1996-08-26 Liposomen mit p-ethoxyoligonukleotiden
EP96929888A EP0847272B1 (en) 1995-08-29 1996-08-26 LIPOSOMAL p-ETHOXY OLIGONUCLEOTIDES
CA2228944A CA2228944C (en) 1995-08-29 1996-08-26 Liposomal phosphodiester, phosphorothioate, and p-ethoxy oligonucleotides
JP51064297A JP4291412B2 (ja) 1995-08-29 1996-08-26 リポソーマルホスホジエステル、ホスホロチオエート、およびｐ−エトキシオリゴヌクレオチド
DE69630691T DE69630691T2 (de) 1995-08-29 1996-08-26 Liposomal p-Ethoxy Oligonucleotides
DK96929888T DK0847272T3 (da) 1995-08-29 1996-08-26 Liposomale p-ethoxy-oligonukleotider

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US08/520,385 1995-08-29
US08/520,385 US5855911A (en) 1995-08-29 1995-08-29 Liposomal phosphodiester, phosphorothioate, and P-ethoxy oligonucleotides

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WO1997007784A3 WO1997007784A3 (en) 1997-04-24

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EP (1) EP0847272B1 (enExample)
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AU (1) AU6912996A (enExample)
CA (1) CA2228944C (enExample)
DE (1) DE69630691T2 (enExample)
DK (1) DK0847272T3 (enExample)
ES (1) ES2210385T3 (enExample)
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DE19740092A1 (de) * 1997-09-12 1999-03-18 Ulrich Dr Med Hengge Liposomen und Verfahren zur Transfektion von Zellen
US6120798A (en) * 1997-06-23 2000-09-19 Alza Corporation Liposome-entrapped polynucleotide composition and method
AU731909B2 (en) * 1997-07-01 2001-04-05 Isis Pharmaceuticals, Inc. Compositions and methods for the delivery of oligonucleotides via the alimentary canal
FR2802422A1 (fr) * 1999-12-21 2001-06-22 Capsulis Structures mixtes resultant de l'incorporation d'une macromolecule biologique, en particulier d'adn, dans une phase structuree d'amphiphiles et vesicules obtenues a partir de ces structures
US6287591B1 (en) 1997-05-14 2001-09-11 Inex Pharmaceuticals Corp. Charged therapeutic agents encapsulated in lipid particles containing four lipid components
US6835395B1 (en) 1997-05-14 2004-12-28 The University Of British Columbia Composition containing small multilamellar oligodeoxynucleotide-containing lipid vesicles
WO2005044998A2 (en) 2003-11-05 2005-05-19 Palingen, Inc. Enhanced b cell cytotoxicity of cdim binding antibody
US6974589B1 (en) 1999-10-08 2005-12-13 Miza Corporation Neutral-cationic lipid for nucleic acid and drug delivery
US6977244B2 (en) 1996-10-04 2005-12-20 Board Of Regents, The University Of Texas Systems Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides
US7005140B2 (en) 2003-03-31 2006-02-28 Alza Corporation Lipid particles having asymmetric lipid coating and method of preparing same
EP0983513A4 (en) * 1997-03-21 2006-05-17 Univ Georgetown LIPOSOMES COMPRISING OLIGONUCLEOTIDES
US7112337B2 (en) 1999-04-23 2006-09-26 Alza Corporation Liposome composition for delivery of nucleic acid
US7262173B2 (en) 1997-03-21 2007-08-28 Georgetown University Chemosensitizing with liposomes containing oligonucleotides
US7285288B1 (en) 1997-10-03 2007-10-23 Board Of Regents, The University Of Texas System Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides
US7390780B2 (en) 1999-04-23 2008-06-24 Alza Corporation Gene delivery mediated by liposome-DNA complex with cleavable peg surface modification
US7704962B1 (en) * 1997-10-03 2010-04-27 Board Of Regents, The University Of Texas System Small oligonucleotides with anti-tumor activity
US9409976B2 (en) 2012-02-08 2016-08-09 Igm Biosciences, Inc. CDIM binding proteins and uses thereof
US9458241B2 (en) 2004-11-05 2016-10-04 Igm Biosciences, Inc. Antibody induced cell membrane wounding
WO2017066643A1 (en) * 2015-10-14 2017-04-20 Bio-Path Holding, Inc. P-ethoxy nucleic acids for liposomal formulation
US9895298B2 (en) 2006-05-03 2018-02-20 Centre National De La Recherche Scientifique (C.N.R.S.) Method for extemporaneous and reversible concentration of liposomes
US10927379B2 (en) 2016-09-16 2021-02-23 Bio-Path Holdings, Inc. Combination therapy with liposomal antisense oligonucleotides
US11041153B2 (en) 2017-04-19 2021-06-22 Bio-Path Holdings, Inc. P-ethoxy nucleic acids for STAT3 inhibition
US12234457B2 (en) 2017-04-19 2025-02-25 Bio-Path Holdings, Inc. P-ethoxy nucleic acids for BCL2 inhibition

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US5855911A (en) * 1995-08-29 1999-01-05 Board Of Regents, The University Of Texas System Liposomal phosphodiester, phosphorothioate, and P-ethoxy oligonucleotides
US6894031B1 (en) * 1996-11-21 2005-05-17 Cedars-Sinai Medical Center Pituitary tumor transforming gene (PTTG) carboxy-terminal peptides and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation
US6565889B2 (en) * 1996-12-02 2003-05-20 The Regents Of The University Of California Bilayer structure which encapsulates multiple containment units and uses thereof
DE60037938T2 (de) 1999-02-26 2009-01-29 The University Of British Columbia, Vancouver Antisense-therapie für trpm-2
US7569551B2 (en) 2000-02-25 2009-08-04 The University Of British Columbia Chemo- and radiation-sensitization of cancer by antisense TRPM-2 oligodeoxynucleotides
US7196067B2 (en) 2000-09-14 2007-03-27 The University Of British Columbia Antisense insulin-like growth factor binding protein (IGFBP)-2-oligodeoxynucleotides for prostate and other endocrine tumor therapy
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DE69630691T2 (de) 2004-11-25
US20040005353A1 (en) 2004-01-08
US5855911A (en) 1999-01-05
ES2210385T3 (es) 2004-07-01
US20070202157A1 (en) 2007-08-30
PT847272E (pt) 2004-04-30
CA2228944C (en) 2011-10-18
CA2228944A1 (en) 1997-03-06
US7176302B2 (en) 2007-02-13
AU6912996A (en) 1997-03-19
DE69630691D1 (de) 2003-12-18
ATE253897T1 (de) 2003-11-15
US7754872B2 (en) 2010-07-13
JP4291412B2 (ja) 2009-07-08
EP0847272B1 (en) 2003-11-12
JPH11512099A (ja) 1999-10-19
WO1997007784A3 (en) 1997-04-24
US6042846A (en) 2000-03-28
DK0847272T3 (da) 2004-03-22

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