WO1993001241A1 - Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus - Google Patents

Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus Download PDF

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Publication number
WO1993001241A1
WO1993001241A1 PCT/FR1992/000621 FR9200621W WO9301241A1 WO 1993001241 A1 WO1993001241 A1 WO 1993001241A1 FR 9200621 W FR9200621 W FR 9200621W WO 9301241 A1 WO9301241 A1 WO 9301241A1
Authority
WO
WIPO (PCT)
Prior art keywords
collagen
skin
fish
unpigmented
flat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1992/000621
Other languages
English (en)
French (fr)
Inventor
Pierre Devictor
Roland Allard
Eric Perrier
Alain Huc
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Beauty Care Solutions France SAS
Original Assignee
Coletica SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coletica SA filed Critical Coletica SA
Priority to DE69221872T priority Critical patent/DE69221872T2/de
Priority to US08/170,353 priority patent/US5420248A/en
Priority to CA002112805A priority patent/CA2112805C/en
Priority to EP92915287A priority patent/EP0592586B1/fr
Publication of WO1993001241A1 publication Critical patent/WO1993001241A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00—Compositions of proteins; Compositions of derivatives thereof
    • C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
    • C08L89/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C08H1/06—Macromolecular products derived from proteins derived from horn, hoofs, hair, skin or leather
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/855—Proteins from animals other than mammals or birds
    • Y10S530/857—Fish; fish eggs; shell fish; crustacea

Definitions

  • non-pigmented fish skin in particular of fish pLat as a new industrial source of collagen, extraction process, collagen and biomaterial thus obtained.
  • the present invention relates essentially to the use of the unpigmented skin of fish, in particular flat fish, as a new industrial source of collagen, a process for the extraction of collagen, as well as collagen and a biomaterial thus obtained.
  • Collagen a protein that supports the connective tissues of living beings, is finding increasing applications in the cosmetic and pharmaceutical fields.
  • the main properties are:
  • the inventors have sought to exploit new sources of collagen making it possible to complete industrial processes, to improve yields, and thereby thereby lower the costs of preparing the protein.
  • the main fabric used to obtain collagen is calf skin.
  • the collection of the skin is generally not carried out under perfect sanitary conditions, which entails the obligation to use the skin as soon as possible after the slaughter of the animal.
  • the pigmented fish skin cannot be used because the pigment is very difficult to remove during the process for preparing collagen.
  • the protein thus obtained then leads to impure pigmented biomaterials. Therefore, the inventors turned to fish whose skin is not pigmented and which is available in large quantities.
  • the species falling into this category are flatfish, in particular those which are caught in an industrial way such as sole, flounder, turbot, catfish, and whose preparation of the nets involves butchering.
  • the ventral skin of these fish is not pigmented, it is available flaking in significant quantities.
  • the present invention relates to the use of non-pigmented fish skin as a new industrial source of collagen.
  • this non-pigmented skin is a flat fish skin, in particular a ventral skin.
  • This non-pigmented skin is advantageously obtained after skinning fresh fish which is frozen immediately after skinning, which guarantees a very good quality of the raw material both bacteriologically and in that of the native character of the protein.
  • an unexpected advantage lies in the fact that the skin thus recovered and frozen can be used immediately for the extraction of collagen without prior tanning work. Also, it turned out that the amount of collagen extracted from this raw material is about three times greater than that obtained from calf skin.
  • the present invention also relates to a process for extracting native collagen, characterized in that non-pigmented fish skin is used which is subjected to collagen extraction.
  • the non-pigmented skin is a flat fish skin, in particular a ventral skin.
  • this skin is a skin obtained after skinning fresh fish which is frozen immediately after skinning. This skin is also used for collagen extraction without prior tanning work.
  • acid-soluble collagen is extracted in the native state by extraction in an acid solution, as is well known to those skilled in the art.
  • the native collagen is then separated by precipitation from the supernatant, by addition of a precipitation agent, for example sodium chloride. If desired, a de-crosslinking of this native collagen can be carried out.
  • the invention also relates to native collagen obtained from the unpigmented skin of fish, and any biomaterial produced wholly, or in part, with such collagen.
  • the industrially fished ventral sole skins are delivered frozen in 20 kg blocks. These are cut into 2 kg blocks using a band saw. The recovered pieces are then crushed and thawed using a knife mill equipped with a 40 l sealed tank. 39 kg of ground material are obtained. 2. Washing of the ground material
  • the ground material is washed with two successive baths of phosphate buffer (21.7 g / l NA-, HP0, and 0.78 g / L of KH-, P0.
  • the concentration in ground material is 1 kg per 5 l of bath and contact time of 1 hour
  • the ground material is separated from the buffer by centrifugation using a continuous decanter rotating at 4000 rpm. phate is then removed under the same conditions by two successive washes with sterile permuted water.
  • This extraction operation is carried out twice.
  • the supernatants are combined and the collagen which they contain is precipitated by the addition of sodium chloride at a concentration of 7%.
  • the fibers obtained are dialyzed against deionized water until the salts are completely eliminated.
  • the collagen obtained can be either returned to an acid solution, or lyophilized.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PCT/FR1992/000621 1991-07-04 1992-07-02 Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus Ceased WO1993001241A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE69221872T DE69221872T2 (de) 1991-07-04 1992-07-02 Verwendung der nichtpigmentierten fischhaut, insbesondere von plattfischen als industrielle herkunft für kollagen. verfahren zur extrahierung, kollagen und biomaterial so erhalten.
US08/170,353 US5420248A (en) 1991-07-04 1992-07-02 Unpigmented fish skin, particularly from flat fish, as a novel industrial source of collagen, extraction method, collagen and biomaterial thereby obtained
CA002112805A CA2112805C (en) 1991-07-04 1992-07-02 Use of unpigmented fish skin, particularly from flat fish, as a novel industrial source of collagen, extraction method, and collagen and biomaterial thereby obtained
EP92915287A EP0592586B1 (fr) 1991-07-04 1992-07-02 Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9108400A FR2678624A1 (fr) 1991-07-04 1991-07-04 Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus.
FR91/08400 1991-07-04

Publications (1)

Publication Number Publication Date
WO1993001241A1 true WO1993001241A1 (fr) 1993-01-21

Family

ID=9414727

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1992/000621 Ceased WO1993001241A1 (fr) 1991-07-04 1992-07-02 Utilisation de la peau non pigmentee de poisson, en particulier de poisson plat comme nouvelle source industrielle de collagene, procede d'extraction, collagene et biomateriau ainsi obtenus

Country Status (9)

Country Link
US (1) US5420248A (https=)
EP (1) EP0592586B1 (https=)
JP (1) JP2722014B2 (https=)
AT (1) ATE157386T1 (https=)
CA (1) CA2112805C (https=)
DE (1) DE69221872T2 (https=)
ES (1) ES2108759T3 (https=)
FR (1) FR2678624A1 (https=)
WO (1) WO1993001241A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2783836A1 (fr) * 1998-08-11 2000-03-31 Hokkaido Government Collagene de poisson et son procede d'extraction et de production
WO2016056949A1 (ru) 2014-10-06 2016-04-14 Общество с ограниченной ответственностью "Системы качества жизни" Способ получения уксуснокислой дисперсии высокомолекулярного рыбного коллагена
CN107573415A (zh) * 2017-10-16 2018-01-12 海南三元星生物科技股份有限公司 一种鱼胶原蛋白的提取方法及其应用

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FR2714063B1 (fr) * 1993-12-20 1996-03-08 Javenech Procédé de préparation de collagène à partir de cnidaires, et compositions obtenues utilisables en cosmétique.
GB9405219D0 (en) * 1994-03-17 1994-04-27 Vickers James Ltd Collagen finings and preparation thereof
JP2731833B2 (ja) * 1995-06-26 1998-03-25 大同ほくさん株式会社 海洋生物を原料とした代用皮膚
US6271350B1 (en) * 1998-08-11 2001-08-07 Thara & Company Ltd. Fish collagen and method of producing same
FR2787968B1 (fr) * 1998-12-31 2001-03-23 Skw Biosystems Procede de preparation de gelatine de poisson
FR2801313A1 (fr) * 1999-05-19 2001-05-25 Coletica Produit collagenique contenant du collagene d'origine marine a faible odeur et de preference a proprietes mecaniques ameliorees, ainsi que son utilisation sous forme de compositions ou de produits cosmetiques ou pharmaceutiques
NZ501386A (en) * 1999-11-29 2002-10-25 Nz Inst For Crop & Food Res Collagen from fish skin
US6790454B1 (en) 2000-05-26 2004-09-14 Coletica Processes for the preparation of novel collagen-based supports for tissue engineering, and biomaterials obtained
FR2809313B1 (fr) * 2000-05-26 2005-11-18 Coletica Procedes de preparation de nouveaux supports a base de collagene pour l'ingenieurie tissulaire et biomateriaux obtenus
FR2809412A1 (fr) 2000-05-26 2001-11-30 Coletica Utilisation de collagene d'origine aquatique pour la realisation de supports destines a l'ingenierie tissulaire, et supports et biomateriaux obtenus
WO2001091821A1 (fr) * 2000-05-26 2001-12-06 Coletica Supports a base de collagene pour l'ingenierie tissulaire et la preparation de biomateriaux
FR2809314B1 (fr) * 2000-05-26 2005-11-18 Coletica Utilisation de collagene d'origine aquatique pour la realisation de supports destines a l'ingenierie tissulaire, et supports et biomateriaux obtenus
US6974679B2 (en) * 2000-05-26 2005-12-13 Coletica Support with collagen base for tissue engineering and manufacture of biomaterials
JP3747412B2 (ja) * 2000-05-26 2006-02-22 エンゲルハード・リヨン 組織工学用にデザインされた担体を製造するための水生動物起源のコラーゲンの使用並びにそれにより得られる担体及び生体材料
KR20020036225A (ko) * 2000-11-09 2002-05-16 이종윤 피부질환 보호-치료용 생체막
ES2212870B1 (es) * 2001-08-08 2005-10-01 Consejo Superior De Investigaciones Cientificas Estabilizacion para la conservacion y almacenamiento a temperatura ambiente de pieles y tejido conectivo de peces, y posterior produccion de colageno y/o gelatina.
US20040078601A1 (en) * 2002-08-02 2004-04-22 Chris Tengwall System and method for operating a wireless device network
PL190737B1 (pl) 2002-10-15 2006-01-31 Przybylski Jozef Edward Sposób otrzymywania kolagenu biologicznie aktywnego ze skór ryb łososiowatych
JP2005087440A (ja) * 2003-09-17 2005-04-07 Matsumoto Shika Univ 海洋生物の原料を含む組成物、及びその組成物の製造方法
JP2005095331A (ja) * 2003-09-24 2005-04-14 Ihara Suisan Kk 魚皮真皮コラーゲンを含有する発泡体シートおよびその用途
US20090312524A1 (en) * 2003-11-28 2009-12-17 Alternative Sourced Collagen, Llc Compositions and methods comprising collagen
US7109300B2 (en) 2004-04-19 2006-09-19 Board Of Supervisors Of Louisiana State Univeristy And Agricultural And Mechanical College Extraction of collagen from calcified tissues
CA2608708C (en) * 2005-05-16 2014-04-22 Purdue Research Foundation Engineered extracellular matrix having a specified fibril area fraction
WO2007106812A2 (en) * 2006-03-13 2007-09-20 University Of South Florida Self-assembling, collagen based material for corneal replacement
WO2007136634A1 (en) * 2006-05-16 2007-11-29 Purdue Research Foundation Three dimensional purified collagen matrices
US20070269476A1 (en) * 2006-05-16 2007-11-22 Voytik-Harbin Sherry L Engineered extracellular matrices control stem cell behavior
CN101091789B (zh) * 2006-06-19 2010-11-10 李勇 海洋胶原肽在制备免疫调节药物或食品中的用途
CN105327393A (zh) 2006-06-22 2016-02-17 南佛罗里达大学 胶原支架、具有其的医用植入物以及使用方法
US8093027B2 (en) * 2006-09-13 2012-01-10 University Of South Florida Method for producing biocomposite comprising collagen and polymer
CA2663722C (en) 2006-09-21 2014-12-09 Purdue Research Foundation Collagen preparation and method of isolation
GB2468102B (en) * 2007-12-10 2012-12-05 Purdue Research Foundation Collagen-based matrices with stem cells
JP2009280508A (ja) * 2008-05-20 2009-12-03 Hikari Ishii 血管老化抑制剤および老化防止抑制製剤
CA2708844C (en) * 2009-07-27 2016-01-05 National Cheng Kung University Preparation of high purity collagen
MX2012004037A (es) * 2009-10-07 2012-10-15 Kerecis Ehf Material de andamiaje para el cuidado de heridas y/u otras aplicaciones para cicatrizacion de tejido.
US9452128B2 (en) 2012-06-06 2016-09-27 Robell Research, Inc. Lip balm
US20140066379A1 (en) * 2012-08-30 2014-03-06 Body Organ Biomedical Corp. Recombinant vector, transgenic fish egg using the same and biomaterial using the same
US9878071B2 (en) 2013-10-16 2018-01-30 Purdue Research Foundation Collagen compositions and methods of use
EP3185922B1 (en) 2014-08-27 2025-04-02 Purdue Research Foundation Collagen-based therapeutic delivery systems
US11919941B2 (en) 2015-04-21 2024-03-05 Purdue Research Foundation Cell-collagen-silica composites and methods of making and using the same
ES3040675T3 (en) 2017-01-31 2025-11-04 Geniphys Inc Methods and compositions for matrix preparation
EP3615568A4 (en) 2017-04-25 2021-01-20 Purdue Research Foundation TISSUE RESTORATION THREE-DIMENSIONAL (3D) ARTIFICIAL MUSCLE
KR102008944B1 (ko) * 2019-06-26 2019-08-08 김경림 전처리 및 추출이 모두 가능한 콜라겐 추출 장치와 이를 이용한 콜라겐 추출 방법
EP4096635A4 (en) 2020-01-27 2024-02-21 GeniPhys, Inc. BIOLOGICAL FILLER FOR TISSUE RESTORATION AND REGENERATION
US20240343759A1 (en) * 2023-04-14 2024-10-17 Fu Yu Hsieh Method of preparing procollagen from freshwater fish

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FR582544A (fr) * 1923-06-07 1924-12-20 Nouvelle matière plastique et procédé pour son obtention
FR647030A (fr) * 1927-01-27 1928-11-19 Procédé perfectionné permettant l'obtention de fibres textiles de haute qualité au moyen de peaux de poissons, tels que et notamment, les chondroptérygiens-sélaciens
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2783836A1 (fr) * 1998-08-11 2000-03-31 Hokkaido Government Collagene de poisson et son procede d'extraction et de production
WO2016056949A1 (ru) 2014-10-06 2016-04-14 Общество с ограниченной ответственностью "Системы качества жизни" Способ получения уксуснокислой дисперсии высокомолекулярного рыбного коллагена
CN107573415A (zh) * 2017-10-16 2018-01-12 海南三元星生物科技股份有限公司 一种鱼胶原蛋白的提取方法及其应用
CN107573415B (zh) * 2017-10-16 2021-09-14 海南华研胶原科技股份有限公司 一种鱼胶原蛋白的提取方法及其应用

Also Published As

Publication number Publication date
FR2678624A1 (fr) 1993-01-08
CA2112805A1 (en) 1993-01-21
ATE157386T1 (de) 1997-09-15
DE69221872D1 (de) 1997-10-02
US5420248A (en) 1995-05-30
EP0592586B1 (fr) 1997-08-27
ES2108759T3 (es) 1998-01-01
FR2678624B1 (https=) 1995-06-02
CA2112805C (en) 2001-04-24
EP0592586A1 (fr) 1994-04-20
JP2722014B2 (ja) 1998-03-04
DE69221872T2 (de) 1998-01-29
JPH06511269A (ja) 1994-12-15

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