WO1989011337A1 - Plaque pour micro-essais - Google Patents

Plaque pour micro-essais Download PDF

Info

Publication number
WO1989011337A1
WO1989011337A1 PCT/DE1989/000304 DE8900304W WO8911337A1 WO 1989011337 A1 WO1989011337 A1 WO 1989011337A1 DE 8900304 W DE8900304 W DE 8900304W WO 8911337 A1 WO8911337 A1 WO 8911337A1
Authority
WO
WIPO (PCT)
Prior art keywords
test plate
micro test
medium
medium containers
plate according
Prior art date
Application number
PCT/DE1989/000304
Other languages
German (de)
English (en)
Inventor
Peter Schulz
Original Assignee
Peter Schulz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Schulz filed Critical Peter Schulz
Publication of WO1989011337A1 publication Critical patent/WO1989011337A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • the invention relates to a micro test plate with a multiplicity of medium containers arranged close to one another, e.g. Recesses, depressions, vessels or the like.
  • micro test plates are used for investigations and used as single-use components. After the tests are completed, such microplates are usually no longer needed, since cleaning and sterilizing them is almost as expensive as manufacturing them. On the other hand, it cannot be ruled out that in one or the other medium container of the microtest plate used, residues of previous media remain, which can influence the measurement results of the subsequent media.
  • the inlet opening of the medium containers must have a certain minimum area in order to be able to achieve usable measured values with the measuring probe.
  • This fact has the consequence that the medium received by the medium container contains a volume several times that which is necessary for the actual examination. This can be partially remedied by keeping the depth of the medium container as small as possible. Above a certain depth, however, surface tensions occur in the medium, which can lead to the bulging of the side of the liquid facing the probe and thus to the measurement results being distorted.
  • the invention has for its object to develop a microtest plate of the type mentioned without undue design effort so that good measurement results can still be achieved while reducing the volume of the media accommodated by individual medium containers. Another object of the invention is to be able to use the micro test plate more effectively.
  • the object is achieved according to the invention in that the medium containers which are open to one side of the microtest plate taper towards their bottoms.
  • the invention is implemented in any case if the side of the measured medium facing the measuring probe is large enough and approximately flat and if the effective volume of the measured medium can be significantly reduced.
  • the side of the medium container facing the measuring probe can also have a significantly larger surface area than a conventional medium container, so that the probe can communicate with a significantly larger surface area of the medium container.
  • the tapering of the medium container from its inlet opening towards the bottom not only minimizes the volume of the medium tested, but also ensures that the side of the medium (liquid) facing the probe is not significantly curved.
  • a particularly expedient embodiment of the invention provides that both sides of the microtest plate have medium containers which are open on one side and which taper from their inlet openings to their bottoms, and that the bottoms on the one side of the micro test plate formed medium container between the inlet openings of the medium container formed on the other side of the micro test plate.
  • the outer sides of the medium containers formed in the bottom or top side form the inner sides of the medium containers formed in the top or bottom side.
  • This embodiment of the invention offers the possibility of using the inner part of the micro test plate as walls of the medium containers.
  • the body of the micro test plate is therefore fully used to hold the medium, which at the same time leads to the material used for the production of the micro test plate being used optimally.
  • these medium containers can be designed in the shape of a truncated cone or pyramid. This exemplary list shows that it is a matter of geometric hollow bodies, the outer sides of which define the envelopes of medium containers on the other side of the microtest plate.
  • the medium containers are separate bodies which can be detachably connected to the base body.
  • Such micro test plates are particularly suitable for sample distribution systems, where the size of the microtest plates is standardized, since the pipette needle (s) can only assume certain positions on a regular basis. If the microtest plate used for such a sample distribution system now has a large number of medium containers, whereby only a part of the medium container is to be used for the test, the medium containers not filled with medium can be detached from the base body of the microtest plate in order to prevent its contamination prevent.
  • Another expedient embodiment of the invention provides that git-shaped locking webs are formed in the top and / or bottom side of the base body, which can be positively connected to the received medium containers.
  • the base body is therefore designed as a lattice-shaped receiving body for the individual medium containers, which can be connected to the base body of the micro test plate as required.
  • a particularly expedient embodiment provides that the top and / or bottom side of the microtest plate can be covered with a film which can be releasably connected to it. Above all, this ensures that the side of the microtest plate that does not receive any medium during the test does not come into contact with the medium either.
  • an expedient embodiment provides that the base body is made of a wear-free and tear-resistant material, while the medium containers are designed as one-way components.
  • FIG. 1 is a perspective side view of a micro test plate with medium containers of different shapes
  • Fig. 5 is a vertical plan view of another micro test plate.
  • FIG. 1 and 5 each show a micro test plate 10 or 12 with a plurality of medium containers 20, 21, 22, 23, 24, 25, 26 arranged close to one another. It can be seen that both sides 30 and 31 of the microtest plate 10 and 12 have medium containers 20, 21, 22, 23, 24, 25 which are open on one side and which taper from their inlet openings to their bottoms.
  • the bottoms of the medium containers 21, 23 and 25 formed on one side 31 of the micro test plate 10 are formed between the inlet openings of the medium containers 20, 22 and 24 formed on the other side 30 of the micro test plate 10.
  • the inlet openings of the medium containers 20, 22 and 24 are formed in one side 30 of the micro test plate, while the inlet openings of the other medium containers 21, 23 and 25 are formed in the other side 31 of the micro test plate.
  • the floors of the Microtest plates are flat, so that they have no appreciable influence on the measurement values that can be determined with measuring probes (not shown).
  • the medium containers 20 and 21 are partially cuboid with a cylindrical extension, while the medium containers 22 and 23 are conical in shape.
  • the medium containers 24 and 25 also consist of a cylindrical part and a polyhedron.
  • FIGS. 2, 3 and 4 show that all medium containers 20 to 25 taper towards their bottoms, which has the advantage that, although the individual medium containers hold relatively little medium, just as good measurement results can be achieved as for medium containers that have a continuous cylindrical or cuboid cross-section that is constant.
  • the basic idea is realized that both the one and the other side of the micro test plate can hold media.
  • the microtest plate 12 shown in FIGS. 5 and 6 consists of closely spaced, conical, actual-shaped medium containers 26 to 29, the bottoms 26 'to 29' of which are arranged in the plane of the bottom side. It can be seen that a medium container 40 on the other side 31 of the micro test plate 12 is arranged between each four medium containers 26 to 29 on one side 30 of the micro test plate 12. From this medium container 40 only the bottom can be seen, which lies in the same plane as the inlet openings of the medium containers 26 to 29. The space between the medium container 40 and the adjacent inlet openings of the medium containers 26 to 29 is filled with material, so that what is to be tested Medium cannot flow between the individual medium containers.
  • the volume of the individual medium containers 26 to regularly amounts to between 100 and 1,000 ml.
  • the matrix-like arrangement of the individual medium containers leads to particularly good utilization of the micro test plate.

Abstract

Plaque pour micro-essais (10) comportant une pluralité de récipients destinés à contenir un milieu, disposés très rapprochés les uns des autres (20, 21, 22, 23, 24, 25), par exemple, des évidements, cavités, godets ou analogues. Les récipients, ouverts en direction d'une face de la plaque pour micro-essais sont de diamètre réduit en direction de leurs bases.
PCT/DE1989/000304 1988-05-20 1989-05-12 Plaque pour micro-essais WO1989011337A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3817181.3 1988-05-20
DE3817181 1988-05-20

Publications (1)

Publication Number Publication Date
WO1989011337A1 true WO1989011337A1 (fr) 1989-11-30

Family

ID=6354756

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1989/000304 WO1989011337A1 (fr) 1988-05-20 1989-05-12 Plaque pour micro-essais

Country Status (2)

Country Link
EP (1) EP0426683A1 (fr)
WO (1) WO1989011337A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3022940A1 (de) * 1979-06-20 1981-04-23 Olympus Optical Co., Ltd., Tokyo Agglutinationsanalysiergefaess
US4599315A (en) * 1983-09-13 1986-07-08 University Of California Regents Microdroplet test apparatus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3022940A1 (de) * 1979-06-20 1981-04-23 Olympus Optical Co., Ltd., Tokyo Agglutinationsanalysiergefaess
US4599315A (en) * 1983-09-13 1986-07-08 University Of California Regents Microdroplet test apparatus

Also Published As

Publication number Publication date
EP0426683A1 (fr) 1991-05-15

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