Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
  • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
  • C07K5/08—Tripeptides
  • C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/54—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one sulfur as the ring hetero atoms, e.g. sulthiame
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
  • A61K38/06—Tripeptides

Definitions

• the present invention relates to a nootropic agent containing a histidylp ⁇ -linamide derivative as a main component.
• the histidylprolinamide derivative according to the present invention can be represented by the following general formula (1).
• R stands for lower-ranked arkir.
• TRH was originally thought to be a hormone that regulates the release of thyrotrobin (TSH) into the pituitary gland of mammals, but recent studies suggest that TRH is not only a release of TSH Rev. Pharmacol. Toxicol. 26, 311-322 (1986)], which has been shown to be widely present in the central nervous system and to have the effect of species *.
• TRH has been found to have various useful effects and to be an effective drug as a nootropic
• TRH is tanned relatively quickly in vivo, It turned out that the transfer to the brain was not always good.
• the action of TRH itself cannot be said to be as strong as expected, and it has been desired to create a derivative having a stronger erect fruit.
• histidyl u ⁇ ' which is a known compound, and which carries the compound of the present invention; ⁇ mid ⁇ conductor has been studied as a substance g which has an effective processing action, for example, after trauma. It has also been proposed to use it as a drug for treating depressive disorders (Japanese Patent Publication No. Sho 61-172828). However, no attempt has been made to identify and study the specific pharmacological effects of the present Kakimaki compound, leading to its commercialization as a pharmaceutical product.
• the present inventors have conducted intensive research on only the compound of the present invention.
• the compound represented by the above general formula [I] is significantly different from TRH in mammals. It has been confirmed that the present invention has an excellent nootropic action, and the present invention has been accomplished.
• the compound of the present invention is a known compound and has a pharmacological action other than a nootropic action.
• the superior nootropic effect of the present invention which may not be sufficiently effective even when administered via birth control,
• they have been found for the first time by the present inventors, and here are important points of the present invention.
• the compound of the present invention can be represented by the above-mentioned general formula (I), wherein R in [I] includes lower alkyl such as methyl, ethyl and propyl. it can.
• Examples of the compound of the present invention include the following compounds.
• the compound of the present invention showed a significant improvement effect in the dose range of 0.05 to (L3 mg / kg), while TRH showed a significant improvement effect in the simple dose range (5 to 30 mgZkg). This compound showed a significant improvement at 1 and 3 mg / kg, even when administered perrole, but did not show any improvement after administration of TRIUi50-lOOmgZfcg. ) Improvement effect on electric shock amnesia
• the compound of the present invention showed a significant improving effect in the dose range of 0.3 to lag / kg, but TRH showed a significant improving effect only for lOmgZkg. ) Improvement effect on cycloheximide amnesia
• the compound of the present invention showed a significant improving effect in the dose range of 0.04 to 0.08 mgZkg, but TRH did not show a significant improving effect in the dose range of 1 to 30 rag / kg.
• the compound of the present invention showed a significant improving effect in the dose range of 0.05 to 3 mg kg, but TRH did not show a significant improving effect in the dose range of 5 to 30 ragZkg.
• the compound of the present invention showed a significant improving effect in the dose range of 0.05 to 0.5 mg / kg, but TRH did not show any improving effect in the dose range of 0.5 to 30 mg / kg.
• test drug was administered intraperitoneally, and 15 minutes later, the rat was left in a chamber filled with carbon dioxide for 12 seconds. Three minutes later, active avoidance and escape learning were performed using a buzzer using a shuttle box as a condition stimulus, and the rate of the 6th trial learning acquisition test (positive animals and used animals) was measured. did. The results are shown in Table 6.
• the compound of the present invention showed a significant improving effect in the dose range of 0 to 3 mg Zkg, but TRH did not show a significant improving effect in the dose range of 10 to 50 mg Zkg.
• Rats with bilateral electrical breakdown of the basal forebrain nucleus were used.
• Condition avoidance learning was performed using a shuttle box. The rat was left in the shuttle box, and after 13 seconds, a buzzer was presented as a conditional stimulus for 3 seconds, and an electric shock (unconditional stimulus) was given through the floor grid with a buzzer for 4 seconds.
• Unconditional stimulation was avoided (conditional avoidance response positive) if the rat moved from the converter on the other side to the one on the other side within 3 seconds of presentation of the conditional stimulation. Twenty times a day, we performed
• the condition avoidance learning acquisition test was started 7 days after basal forebrain rupture, and the condition avoidance response rates for the following 10 days are shown in Table 7.
• the compound of the present invention showed a significant learning-obtaining effect at 0.1 to lmg / kg, but the TRH was not suitable for the significant learning-obtaining effect only in the lOmgZkg-administered group.
• a radial maze with eight arms was used. A small piece of cheese was placed on the tip of each arm as a bait reward. Place the hungry rat in the center of the radiant maze and at the end of the arm You can freely take the bait reward. However, the bait reward was placed in a depression, unlike the rat in the middle of the maze. In this task, the la-not selects the arm where the bait report is placed, and if the bait report gets ugly, it is regarded as a positive choice, and there are days when the number of positive choices out of 8 is 7 or more. Kneading was carried out until 3 or more continuations
• the present compound showed significant improvement after intraperitoneal administration of 0.3 mg kg / kg and lmg / fcg in vitro, but TRH in the intraperitoneal administration of 3-30 Bg Zkg resulted in a slight improvement. No significant improvement was observed at the dose.
• Physostigmine (indicated as PH in Table 8) showed a significant improvement in the intraperitoneal administration of O. Sag Z kg.
• a T-shaped maze was used.
• the device consists of an ffi departure box, a runway and a target box.Each slot has a guide pin.
• a reward was given (a piece of cheese).
• the rat was returned to the departure box with the guillotine door closed, and hesitated after a five-second delay.
• the rat In the case of a selective run, the rat is also placed in the target box that is offset to the left and right, but a bait reward is given only when the player enters a target box different from the one in which the bait reward was obtained in the forced run. Was done.
• the compound of the present invention significantly improved the learning disability of this task due to scopolamine with a delay time of 5 30 s 120 and 480 seconds at 0.3 mgZkg, whereas physostigmine had a delay time of 5 seconds at 0.5 mg Zkg. Only a significant rewriting effect was shown.
• the compound of the present invention The efficacy is clear and its efficacy is not only injection (intravenous, intravenous, intravenous, subcutaneous, intramuscular), topical (rectal, sublingual, intranasal, etc.) It is clear that this occurs well upon administration.
• the toxicity of the compound of the present invention was observed for 7 days after intravenous administration and intramuscular administration of the compound of the present invention to male mice.
• the compound of the present invention When the compound of the present invention is administered as a medicament, the compound of the present invention is used as it is or in a pharmaceutically acceptable non-toxic or inactive carrier, for example, as an injection, 0.0001 to 1.2%. It is administered to animals including humans as a pharmaceutical composition having 0.0001 to 2% as a tablet, preferably 0.01 to 1% as an injection, and 0.001 to 1% as a tablet.
• the pharmaceutical composition is preferably administered in a dosage form.
• Book The pharmaceutical composition of the present invention can be administered transdermally, intradermally, topically (eg, transdermally), or rectally. Needless to say, it is administered in a dosage form suitable for these administration methods.
• Example Eba, route of administration is particularly Konomashii ⁇
• the dose as a nootropic agent should be adjusted in consideration of the patient's condition such as age, weight, etc., administration route, and the nature and extent of the disease.
• the amount of the active ingredient of the present invention is generally in the range of 0.01 to 50 otg per day, preferably in the range of 0.1 to 10 mg per day. In some cases, lower doses may be sufficient, and conversely, higher doses may be required. It is also desirable to administer the drug several times a day.
• ⁇ ⁇ administration is in solid or liquid dosage units, for example, powders, powders, tablets, dragees, capsules, granules, clouding agents, liquids, tablets, tablets, Sublingual tablets and other dosage forms can be used.
• Powders are prepared by comminuting the active substance to an appropriate degree. Powders are prepared by comminuting the active substance with an appropriate fineness and then admixing to a similarly finely divided pharmaceutical carrier, for example, starch, edible carbohydrates such as mannitol, and the like. If necessary, flavors, preservatives, dispersants, coloring agents, fragrances and other things can be used.
• a similarly finely divided pharmaceutical carrier for example, starch, edible carbohydrates such as mannitol, and the like.
• a similarly finely divided pharmaceutical carrier for example, starch, edible carbohydrates such as mannitol, and the like. If necessary, flavors, preservatives, dispersants, coloring agents, fragrances and other things can be used.
• the capsule is a powdered powder obtained from the above-mentioned powder, powder or granulated as described in the section on tablets, for example, a capsule outer shell made of gelatin capsule.
• Lubricants and fluidizers such as colloidal silica, talc, magnesium stearate, calcium stearate, solid polyethylene glycol, and the like are mixed with the powdery one, Sufficient operation can be performed later.
• Capsules can be made by adding a disintegrant or solubilizer, for example, carboxymethylcellulose, carboxymethylcellulose calcium, low-substituted hydroxypropyl cellulose, calcium carbonate, or sodium carbonate. Improving the impeachment of the doctor when ingested.
• the fine powder of this product can be suspended and dispersed in vegetable oil, polyethylene glycol, glycerin, and a surfactant, and wrapped with a gelatin sheet to prepare a soft capsule. Tablets are made into a powder mixture, granulated or sluged, and then subjected to compression with the addition of a disintegrant or lubricant in the following step.
• the powder mixture is made into a suitable powder. ⁇ is mixed with the above-mentioned diluent base, and if necessary, a binder (for example, carboxymethylcell ⁇ -snadium, alginic acid, gelatin, polyvinyl viridone, polyvinyl alcohol, etc.) ), Dissolution retarder (such as paraffin-paraffin), reabsorbent (such as quaternary salt), and adsorbent (such as paraben sodium, kaolin, dicalcium phosphate)
• a binder such as syrup, starch paste, arabia gum, cellulose solution or ⁇ molecular material ⁇ solution
• the powder can be strongly passed through a sieve to obtain granules.
• the granules made in this way can prevent mutual adhesion.
• the lubricated mixture is then tableted in the next step, and the drug is mixed with a fluid inert carrier without going through the granulation and slagging steps described above. It is okay to press directly afterwards.
• a transparent or translucent protective coating consisting of a sealing coating of Shraq, a coating of sugar molecular material, and a polishing coating S consisting of only a small amount are also used.
• parenteral dosage forms such as, for example, rabbit liquid, syrups, elixirs, etc.
• Syrup is produced by dissolving the compound in an appropriate flavored water solution
• elixir is produced by using a non-toxic alcoholic carrier. Boxes are formulated by dispersing the compound in a non-toxic carrier.
• Solubilizing agent emulsification
• boroxixethylenrubitol tolesters preservatives
• flavoring agents for example, bamboo oil, saccharin
• Others can also be added as needed.
• microdose formulations for parenteral administration may be used.
• the formulation can also provide a sustained release of action time upon application or incorporation into biomolecules *.
• liquid dosage forms such as subcutaneous, intramuscular, or intravenous injections, such as a liquid suspension or suspension
• a liquid suspension or suspension may be used.
• a nontoxic liquid carrier suitable for the purpose of injection such as an aqueous or oleaginous medium
• Purul may take a fixed amount of the compound in a vial and then sterilize and seal the vial and its contents.
• a preliminary vial carrier may be prepared in addition to the powdered or lyophilized active ingredient.
• a non-toxic salt solution may be added to make the injection solution isotonic.
• compounds such as stabilizers, preservatives and emulsifiers can be used in combination.
• compounds which are soluble or insoluble in water having a low melting point such as polyethylene glycol, cocoa butter, and esters (For example, myristylester palmitate) and a suppository containing a mixture thereof.
• the formulation of the compound of the present invention includes the active ingredient according to the present invention.
• other drugs for example, other antacids, histamine antagonists, etc. may be used in combination. The best way to carry out a fortune

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
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• General Health & Medical Sciences (AREA)
• Medicinal Chemistry (AREA)
• Veterinary Medicine (AREA)
• Pharmacology & Pharmacy (AREA)
• Public Health (AREA)
• Animal Behavior & Ethology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Epidemiology (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Immunology (AREA)
• Gastroenterology & Hepatology (AREA)
• Genetics & Genomics (AREA)
• Molecular Biology (AREA)
• Biophysics (AREA)
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• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

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Citations (4)

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• 1988
  • 1988-04-07 CH CH4571/88A patent/CH673654A5/de not_active IP Right Cessation
  • 1988-04-07 US US07/290,050 patent/US4956364A/en not_active Expired - Lifetime
  • 1988-04-07 GB GB8827321A patent/GB2219501B/en not_active Expired - Fee Related
  • 1988-04-07 KR KR1019880701624A patent/KR960002182B1/ko not_active Expired - Fee Related
  • 1988-04-07 EP EP88903353A patent/EP0382842B1/en not_active Expired - Lifetime
  • 1988-04-07 WO PCT/JP1988/000347 patent/WO1988007867A1/ja not_active Ceased
  • 1988-04-07 DE DE88903353T patent/DE3884402T2/de not_active Expired - Fee Related
  • 1988-04-07 NL NL8820210A patent/NL8820210A/nl not_active Application Discontinuation
  • 1988-12-07 DK DK681288A patent/DK681288A/da not_active Application Discontinuation
  • 1988-12-08 SE SE8804431A patent/SE8804431D0/xx not_active Application Discontinuation

Patent Citations (4)

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