WO1988005819A1 - RECOMBINANT PLASMID DNA pPR-IFNbeta1-13, CODING FOR SYNTHESIS OF HUMAN FIBROBLAST beta1-INTERFERON, METHOD OF ITS CONSTRUCTION AND STRAIN OF BACTERIA ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) AS PRODUCER OF HUMAN beta1-INTERFERON, CONTAINING IT - Google Patents
RECOMBINANT PLASMID DNA pPR-IFNbeta1-13, CODING FOR SYNTHESIS OF HUMAN FIBROBLAST beta1-INTERFERON, METHOD OF ITS CONSTRUCTION AND STRAIN OF BACTERIA ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) AS PRODUCER OF HUMAN beta1-INTERFERON, CONTAINING IT Download PDFInfo
- Publication number
- WO1988005819A1 WO1988005819A1 PCT/SU1988/000033 SU8800033W WO8805819A1 WO 1988005819 A1 WO1988005819 A1 WO 1988005819A1 SU 8800033 W SU8800033 W SU 8800033W WO 8805819 A1 WO8805819 A1 WO 8805819A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- dηκ
- ppr
- interferon
- ρρκ
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
Definitions
- the area of technology is not limited to the field of genetic engineering and biotechnology, and more specifically.
- the synthesizer used in this case provides an intricate mixture of its various products and functions, which is a result of a complicated operation. This essentially complicates the following properties of individual interactions. ⁇ connection with it more and more
- the appliance receives the input of individual components from the microprocessor-friendly synthesis.
- the invention is also a method of combining a p-combination plasmid for D- ⁇ ⁇ 13 ⁇ -13, which is also true for ⁇ ⁇ 24 north,
- the indicated, the wiring is also the headquarters of the Bacterium ⁇ Igenetica ⁇ 903 ( ⁇ - ⁇
- the strain is claimed to ensure that the person is safe to eat and / or to eat and / or drink;
- the method of eliminating the declared industrial requirement for the maintenance of ⁇ > 1-13 is not limited to several stages.
- the ⁇ - ⁇ zh 1> 1-123 means that the proximity of the ST-sequential gene is closed for the second / second gene
- the transformer will receive cells ⁇ . ⁇ ⁇ ⁇ 600, which eliminates the stress on the medium with an amperage with a direct voltage and a voltage of 28 ° ⁇ .
- the battery is suitable for a temperature range of 5 to 40 ° C (optimum 35 ° C) for 6.7-7.5.
- ⁇ ⁇ aches ⁇ ve is ⁇ chnzh ⁇ a ugle ⁇ da and ⁇ lzuyu ⁇ amin ⁇ zhsl ⁇ y w uglev ⁇ dy (na ⁇ ime ⁇ , sa ⁇ a ⁇ zu) 20 Is ⁇ chni ⁇ m az ⁇ a m ⁇ gu ⁇ sluzhi ⁇ mzhne ⁇ alnye s ⁇ li in nzhyn ⁇ y ⁇ me atm and ⁇ a ⁇ zhe ⁇ ganzhches ⁇ zhe s ⁇ edzhenzhya in vzhde ⁇ e ⁇ na, ⁇ zh ⁇ na, d ⁇ zhzhev ⁇ g ⁇ e ⁇ s ⁇ a ⁇ a w amin ⁇ isl ⁇ Us ⁇ ychzhv ⁇ s ⁇ ⁇ an ⁇ zhbzh ⁇ zh ⁇ am.
- the strain is stable to concentrate up to 100 mg / l and shruzhzhvanzhi 25 burned on agazirovannyh nutritional
- Plasma stability P ⁇ zh ⁇ anenzhzh ⁇ le ⁇ on ar ⁇ iz ⁇ vann ⁇ y s ⁇ ede (s ⁇ m I d ⁇ months) ⁇ i se ⁇ zhzh ⁇ sl d ⁇ va ⁇ elny ⁇ ⁇ e ⁇ esev ⁇ v (in ⁇ echenie at least 6 months) in ⁇ tsesse glubzhnn ⁇ g ⁇ ⁇ ul ⁇ ivzh ⁇ vanzhya in zhzhd ⁇ y s ⁇ ede 30 an ⁇ zhbi ⁇ zh ⁇ m not ⁇ zhs ⁇ di ⁇ ⁇ e ⁇ i Well ⁇ e ⁇ es ⁇ y ⁇ zh ⁇ lazm dy.
- Phase I In fact, even less than the last plasmid is 12 V.
- 2 mcg of the plasmid ⁇ 24 24 enlarges the local distribution of excrement in 40 flop of the isolation buffer ⁇ .
- the accessory 3--parts of the participants of the Département I ⁇ . s ⁇ in ⁇ be ⁇ bem ⁇ m m ⁇ l 20, 10 s ⁇ de boiling m ⁇ ⁇ is- ⁇ S ⁇ , ⁇ 8,0, 10 m ⁇ m ⁇ 2, 2 m ⁇ g ⁇ e ⁇ a D ⁇ , ⁇ 30 m ⁇ dez ⁇ si ⁇ zhb ⁇ nu ⁇ le ⁇ zzhd ⁇ zh ⁇ s ⁇ a ⁇ v 5 edzh ⁇ e ⁇ men ⁇ a.
- Two of the reactive mixtures transfer ethane and dissolve in 100 ⁇ l ⁇ ⁇ ⁇ .
- the resulting mixture is the translucent bore gasket of the C600 C600.
- the accessory of the transformer was composed up to 5 » 10 at the same level as the first one at a time ⁇ 24. It obstructs clones that are stable for amplification (100 mcg / ml), but they also emit a separate module for modifying
- the received message also contains a fragment ( ⁇ ⁇ mulegi ⁇ ) that burns into the receiver ⁇ 24 ⁇ .
- the obtained product is in the range of 150 ⁇ l, containing 50 mt of trap-n ⁇ terrorism, ⁇ 8.0, 10 mk ⁇ of ethylenejaminetetus, as a result
- the remote end participants have been remotely operated by an end user of 100 ⁇ l, which is 2 at a time.
- the nucleotide installed by the method of Sengar, the investigation of the hybrid participation of the communal food in the structure of the device, is the following:
- Shamazmidu ⁇ ⁇ - ⁇ / 31-13 which supports the synthesis of a human being, has an analogous connection to the bathroom, which is included in the manual
- 35 ⁇ 903 is around 10 slots on the 1st week of August - 16 - ⁇ ⁇ whatsoever ⁇ Operations ⁇ - ⁇ - ⁇ . Particles are removed from a medium containing amperage (100 ⁇ g / ml) after a fresh bulb is removed at a temperature of 28 ° C. From all the oblique, they isolate plasmid DB
- the facility is equipped with a fully equipped facility.
- the medium is available at 100 ⁇ g / ml and 10 g / l glucose.
- the seed crop is introduced in the range of 5-10 ma.
- the 25th is the level of supply of ammonia water. Part Pe ⁇ vuyu ⁇ e ⁇ men ⁇ atszhzh ⁇ v ⁇ dya ⁇ ⁇ zh ⁇ em ⁇ e ⁇ a ⁇ u ⁇ e 28 ° C d ⁇ ⁇ zhches ⁇ ⁇ l ⁇ n ⁇ s ⁇ zh, ⁇ avn ⁇ y 3.5 ⁇ i 550 nm and za ⁇ em ⁇ susches ⁇ vlyayu ⁇ ⁇ e ⁇ d ⁇ zhndu ⁇ zhyu, ⁇ vyshaya ⁇ em ⁇ e ⁇ a ⁇ u ⁇ u d ⁇ 42-45 ° C in a 5 ⁇ echenzh mzhn, ⁇ sle cheg ⁇ ⁇ d ⁇ lzhayu ⁇ ⁇ e ⁇ len ⁇ atszhyu still chasa 2 ⁇
- Zayavlyaemyzh sh ⁇ amm ba ⁇ e ⁇ y ⁇ zs ⁇ eg ⁇ s ⁇ and s ⁇ ⁇ IIgene ⁇ i ⁇ a ⁇ ⁇ 903 ( ⁇ - ⁇ Y ⁇ - ⁇ z> - ⁇ dutsen ⁇ /> 1-iya ⁇ e ⁇ e ⁇ on chel ⁇ ve ⁇ a na ⁇ di ⁇ ⁇ zhmenenie in mi ⁇ bi ⁇ l ⁇ giches ⁇ y and meditsins ⁇ y ⁇ myshleyan ⁇ s ⁇ i ⁇ lucheniya for 1 h 15 in ⁇ e ⁇ e ⁇ na l ⁇ ve ⁇ a, ⁇ y m ⁇ zhe ⁇ ⁇ imenya ⁇ sya in meditsins ⁇ y ⁇ a ⁇ i ⁇ e .
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19883890050 DE3890050T1 (de) | 1987-02-09 | 1988-02-08 | Rekombination-plasmid-dns ppr-ifn(beta)1-13 die die synthese des menschlichen fibroblast-(beta)1-interferons kodiert, verfahren zum konstruieren derselben und stamm der bakterien |
HU881692A HUT50871A (en) | 1987-02-09 | 1988-02-08 | Process for producing recombinant plasmid dna encoding human fibroblast-beta 1-interferon and escherichia coli comprising same |
NL8820103A NL8820103A (nl) | 1987-02-09 | 1988-02-08 | Recombinant plasmide dna - phr-ifnbeta 1-13 coderende synthese van fibroblastisch humaan beta1-interferon, werkwijze ter bereiding ervan en een stam van de bacterie escherichia coli vniigenetika vl 903 (phr-ifnbeta1-13), die humaan beta1-interferon vormt, die dit bevat. |
FI884556A FI884556A0 (fi) | 1987-02-09 | 1988-10-04 | Rekombinantplasmid-dna ppr-ifn 1-13, som kodas foer syntes av maenniskans fibroblast- 1-interferon, foerfarande foer framstaellning daerav och e.coli innehaollande detta. |
GB8823728A GB2208866A (en) | 1987-02-09 | 1988-10-10 | Recombinant plasmid dna ppr-ifn¼-13, coding for synthesis of human fidroblast ¼-1 interferon, and strain of bacteria escherichia coli vniigenetika vl903 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4191345/28 | 1987-02-09 | ||
SU874191345A SU1703692A1 (ru) | 1987-02-09 | 1987-02-09 | Рекомбинантна плазмидна ДНК @ -1 @ 1-13, кодирующа синтез фибробластного интерферона ( @ I) человека, способ ее конструировани , штамм бактерий ЕSснеRIснIа coLI - продуцент @ I-интерферона человека |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1988005819A1 true WO1988005819A1 (en) | 1988-08-11 |
Family
ID=21284355
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SU1988/000033 WO1988005819A1 (en) | 1987-02-09 | 1988-02-08 | RECOMBINANT PLASMID DNA pPR-IFNbeta1-13, CODING FOR SYNTHESIS OF HUMAN FIBROBLAST beta1-INTERFERON, METHOD OF ITS CONSTRUCTION AND STRAIN OF BACTERIA ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) AS PRODUCER OF HUMAN beta1-INTERFERON, CONTAINING IT |
Country Status (9)
Country | Link |
---|---|
JP (1) | JPH01502478A (fi) |
CH (1) | CH676996A5 (fi) |
FI (1) | FI884556A0 (fi) |
GB (1) | GB2208866A (fi) |
HU (1) | HUT50871A (fi) |
NL (1) | NL8820103A (fi) |
SE (1) | SE8803567D0 (fi) |
SU (1) | SU1703692A1 (fi) |
WO (1) | WO1988005819A1 (fi) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69219775T3 (de) * | 1992-10-14 | 2004-08-05 | Ajinomoto Co., Inc. | Neuartiges L-threoninproduzierendes Mikrobakterium und eine Herstellungsmethode für L-Threonin |
CA2591235C (en) * | 2004-12-20 | 2014-03-11 | Cadila Healthcare Limited | Process for preparing high levels of interferon beta |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4582800A (en) * | 1982-07-12 | 1986-04-15 | Hoffmann-La Roche Inc. | Novel vectors and method for controlling interferon expression |
-
1987
- 1987-02-09 SU SU874191345A patent/SU1703692A1/ru active
-
1988
- 1988-02-08 HU HU881692A patent/HUT50871A/hu unknown
- 1988-02-08 NL NL8820103A patent/NL8820103A/nl unknown
- 1988-02-08 JP JP50208988A patent/JPH01502478A/ja active Pending
- 1988-02-08 WO PCT/SU1988/000033 patent/WO1988005819A1/ru active Application Filing
- 1988-02-08 CH CH381588A patent/CH676996A5/de not_active IP Right Cessation
- 1988-10-04 FI FI884556A patent/FI884556A0/fi not_active Application Discontinuation
- 1988-10-07 SE SE8803567A patent/SE8803567D0/xx not_active Application Discontinuation
- 1988-10-10 GB GB8823728A patent/GB2208866A/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4582800A (en) * | 1982-07-12 | 1986-04-15 | Hoffmann-La Roche Inc. | Novel vectors and method for controlling interferon expression |
Non-Patent Citations (2)
Title |
---|
"Biotekhnologia", pod, red. A.A. BAEVE, 1984, Nauka, (Moscow), pages 204-205. * |
ZHURNAL VSESOJUZNOGO KHIMICHESKOGO OBSCHESTVA IM. D. I. MENDELCEVA, tom XXIX Nr. 2, 1984, (Khimia, Moscow), E.D. SVERDLOV, "Interferony i Geneticheskaya Inzheneria", pages 86-89, (206-209). * |
Also Published As
Publication number | Publication date |
---|---|
SE8803567L (sv) | 1988-10-07 |
FI884556A (fi) | 1988-10-04 |
NL8820103A (nl) | 1989-01-02 |
CH676996A5 (fi) | 1991-03-28 |
JPH01502478A (ja) | 1989-08-31 |
FI884556A0 (fi) | 1988-10-04 |
SU1703692A1 (ru) | 1992-01-07 |
SE8803567D0 (sv) | 1988-10-07 |
GB2208866A (en) | 1989-04-19 |
HUT50871A (en) | 1990-03-28 |
GB8823728D0 (en) | 1988-12-07 |
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