Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/52—Cytokines; Lymphokines; Interferons
  • C07K14/54—Interleukins [IL]
  • C07K14/55—IL-2
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
  • C12N15/70—Vectors or expression systems specially adapted for E. coli
  • C12N15/73—Expression systems using phage (lambda) regulatory sequences

Definitions

• Interleukin-2 is an immune modulator of the complex mechanism of action, in particular, it stimulates an immune response due to the activation of comparatively jointly-lymphocytes . , amplification of mitogenesis of stimuli, induction of cytotoxic activity of ⁇ -cells and others.
• the alternative source of interleukin-2 can be used by genetic engineering methods of the microorganism strain, - 2 - carrying regulatory plasmids with the gene of interleukin-2 and ensuring effective expression of this gene.
• the recombinant plasmid Ds which encompass the interleukin-2 human, are known as the recombinant plasmid 5, and the second one is known.
• the means of cooperation are declared recombinant
• 25 plasmid D is included in that, that plasmid ⁇ -23, with a size of 5.4 thousand tons ⁇ . associated with plasmid ⁇ -322 and containing SOS-replica, the gene for the sustainability of acicillin and the second gene of the patient is 2-patient
• SOS modifies by replacing the part of the product for Part I on the site of the distribution in ⁇ II received shimazhu ⁇ 2 ⁇ Z-23 ⁇ is operating;
• the invention is also a strain of the United States Generator 90 903 ( ⁇ - ⁇ 2- ⁇ 9) ⁇ additive producer of an inteleukin-2 people-35 eyelids, which contains a 112-year ⁇ old patient. According to the invention, a method has been obtained. - 6 - for genetic contagion through the introduction of a recumbent plaque ⁇ ⁇ ⁇ ⁇ 2 2- ⁇ 9 ⁇ bacterium ⁇ ⁇ ⁇ ⁇ ⁇ till ⁇ ⁇ .
• the mixture obtained means that the cells are cleaned and that they are inoculated with a medium with an amperage of 15 killin and are secured at a temperature of 28 ° C.
• ⁇ b ⁇ anny ⁇ ⁇ a ⁇ im ⁇ b ⁇ az ⁇ m ⁇ l ⁇ ya ⁇ v vydelyayu ⁇ ⁇ lazmidnuyu D ⁇ ⁇ b ⁇ znachennuyu ⁇ a ⁇ ⁇ - ⁇ 2- ⁇ 9, in ⁇ y ⁇ chn ⁇ s ⁇ v ⁇ ss ⁇ edineniya ⁇ ashen ⁇ v ⁇ ve ⁇ yayu ⁇ v ⁇ ss ⁇ an ⁇ vle- 20 Niemi on s ⁇ y ⁇ e is ⁇ dny ⁇ uchas ⁇ v D ⁇ ⁇ anee ⁇ su ⁇ s ⁇ vuyuschey ⁇ sled ⁇ va ⁇ eln ⁇ s ⁇ i recognition ⁇ es ⁇ i ⁇ azy ⁇ z ⁇ .
• the claimed sh ⁇ amm ⁇ .s ⁇ ⁇ IIgene ⁇ i ⁇ a ⁇ 903 ( ⁇ - ⁇ 2- ⁇ luchayu ⁇ ⁇ ans ⁇ matsiey ⁇ etsi ⁇ ien ⁇ n ⁇ g ⁇ sh ⁇ amma ba ⁇ e ⁇ y ⁇ zs ⁇ eg ⁇ s ⁇ a s ⁇ ⁇ e ⁇ mbinan ⁇ n ⁇ y ⁇ lazmidn ⁇ y D ⁇ 25 ⁇ - ⁇ 2- ⁇ 9 ..
• the Storm of E. genetics ⁇ 903 ( ⁇ - ⁇ 2- ⁇ 9) is characterized by the following symptoms.
• the battery is damaged by a simple and liquid synthetic, semi-synthetic, and complex environment.
• the boulevard When growing on an aggre- gated boulevard in Hortingburg, the boulevard will develop smooth, round, weak 20 worn out mild formations.
• When cultivated in a liquid it is a type of bouillon or ⁇ 9 with an open-ended mixture, and a similar suspension is obtained.
• the batteries are suitable for a range of temperatures from 5 to 40 ° C (optimum 37 ° C) 25 to 6.7-7.5.
• carbohydrates for example, sugar
• amino acids are used as a source of carbon.
• nitrogen can serve as mineral salts in the ammonium form, as well as organic compounds in the form of a substance, a substance, a burned 30 amine.
• the strain is susceptible to amperage at a concentration of up to 100 mg / l and cultivated in liquids and on aggre-ated nutritional products.
• 3 mcg of plasmid ⁇ 40 promotes the release of 1 ⁇ 1 ⁇ in 60 ⁇ l of buffer for restorative- ⁇ containing
• DBD is disposed of for volume of this alcohol.
• the plant is 10 msec ⁇ 2 0.
• the processing plant is 30 msec in size, and contains 30 msec.
• Plasma plasmid ⁇ institutions24 expands the products and the other type of Bacillus in 70 ⁇ l of buffer for Test- ⁇ , containing 10 ⁇ pis- ⁇ , ⁇ 7.9, 6 m ⁇ 2 , 6
• the efficiency of the transmutation makes up 5 * 10 per I ig of the native plasmid ⁇ 24 • Stops obliques that are stable to ampicillin (100 g / ml), from below
• plasmid ⁇ 2 ⁇ -23 ⁇ is produced.
• the plasmid ⁇ 2 ⁇ -3 with a size of 5.4 thousand n.p., is based on the basic plasmid ⁇ -322 and
• the linearized plasmid ⁇ 24 ⁇ is combined with the plasmid fragment ⁇ 2 ⁇ -23 ⁇ .
• I mcg plasmid J and 2 mcg fragment process the J-ligase phage ⁇ 4 in the bushe for 15 ligations and the volume of the reaction mixture is 30 ⁇ l.
• the plasmid J is isolated, as described above, and advances the direct analysis.
• the cultural density of the seed crop is 1.5–2.5 units.
• the product is equipped with a control system equipped with a temperature regulating system
• 35 for optical density equal to 3.5 at 550 nm, at a temperature of 28 ° C, and then carry out the thermal induction, increasing - 15 - a temperature of 42-45 ° C for 5 minutes, after which they continue for another 2 hours.
• a synthetic human protein was received from the integrated cell protein from the I ml of the commercial liquid, and the catering is supplied.
• the product analyzes the elec- trophoresis in a 15% polylamide gel in the presence of a 0.1% sodium sulfide solution. Proteins separated in le are parsed in the factory of the Humassi 250-250 Zegrá / ⁇ standard method. The white content of whites in the area is divided after scanning on the densitel gel.
• the zone of interleukin appears in the form of a brown cover for an unfulfilled window of a non-filtering cell.
• the synthesized protein products of the gene for interleukin-2 it makes up to $ 12 of the total amount of the battery in the cell. Intended use
• the inventive recombinant plasmid J ⁇ - ⁇ 2- ⁇ - 16 - cadreuyu synthesis of interleukin-2 people find an application for receiving strains-beneficiaries of interleukin-2 people with high activity.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Genetics & Genomics (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
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• Bioinformatics & Cheminformatics (AREA)
• Biotechnology (AREA)
• General Engineering & Computer Science (AREA)
• Molecular Biology (AREA)
• General Health & Medical Sciences (AREA)
• Biophysics (AREA)
• Biochemistry (AREA)
• Wood Science & Technology (AREA)
• Biomedical Technology (AREA)
• Physics & Mathematics (AREA)
• Microbiology (AREA)
• Plant Pathology (AREA)
• Virology (AREA)
• Toxicology (AREA)
• Gastroenterology & Hepatology (AREA)
• Medicinal Chemistry (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Preparation Of Compounds By Using Micro-Organisms (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

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Citations (3)

• 1987
  • 1987-02-09 SU SU874191353A patent/SU1703693A1/ru active
• 1988
  • 1988-02-08 DE DE883890052T patent/DE3890052T1/de not_active Withdrawn
  • 1988-02-08 WO PCT/SU1988/000032 patent/WO1988005818A1/ru not_active Ceased
  • 1988-02-08 HU HU881691A patent/HUT50873A/hu unknown
  • 1988-02-08 JP JP88502088A patent/JPH01502639A/ja active Pending
  • 1988-02-08 NL NL8820102A patent/NL8820102A/nl not_active Application Discontinuation
  • 1988-02-08 CH CH3816/88A patent/CH676997A5/de not_active IP Right Cessation
  • 1988-10-04 FI FI884557A patent/FI884557A0/fi not_active Application Discontinuation
  • 1988-10-07 SE SE8803566A patent/SE8803566L/ not_active Application Discontinuation
  • 1988-10-10 GB GB8823729A patent/GB2210373A/en not_active Withdrawn

Patent Citations (3)

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