WO1988005467A1 - Process for the preparation of xylitol from xylose by cultivating candida guilliermondii - Google Patents
Process for the preparation of xylitol from xylose by cultivating candida guilliermondii Download PDFInfo
- Publication number
- WO1988005467A1 WO1988005467A1 PCT/FI1987/000162 FI8700162W WO8805467A1 WO 1988005467 A1 WO1988005467 A1 WO 1988005467A1 FI 8700162 W FI8700162 W FI 8700162W WO 8805467 A1 WO8805467 A1 WO 8805467A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- xylose
- xylitol
- medium
- yeast
- preparation
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
Definitions
- the object of the present invention is a microbiological process for the preparation of xylitol from xylose con ⁇ taining mixtures using the yeast strain Candida guillier ⁇ mondii VTT-C-71006.
- Xylitol is conventionally prepared on a technical scale by hydrolysing hemicellulose containing substances, for example birch wood, whereby a pentose rich hydrolysate is obtained.
- the xylose of the hydrolysate can thereafter be hydrogenated catalytically to form xylitol.
- microbiological methods for the production of xylitol are prior known.
- H. Onishi & T. Suzuki "The Production of Xylitol, L-Arabinitol and Ribitol by Yeasts", Agr. Biol. Chem., Vol. 30, No. 11, p. 1139 to 1144, 1966, is disclosed the microbiological production of xylitol from a xylose containing solution by the yeast species Candida guilliermondii.
- the initial xylose con ⁇ centration of the solution used as raw material was 10 % by weight.
- the yield of xylitol was 40.3 %.
- the strain Candida guilliermondii VTT-C-71006 is deposited in the strain collection at National Collection of Yeast Cultures (NCYC), Norwich, England, on 17th December 1986 under no NCYC-1644.
- Candida guilliermondii VTT-C-71006 exhibits the following morphological and physiological properties.
- cultivation is carried out by cultivating the strain Candida guilliermon- dii VTT-C-71006 preferably under aerated conditions in a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
- a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
- Suitable raw materials are any xylose containing mixtures, for example the xylose rich mixtures obtained from the hydrolysis of hemicellulose containing materials.
- the fermentation solution as the N-source for the yeast different ammonium salts may be used and as a growth fac ⁇ tor source for example yeast extract, yeast produced in the process or extract of plant origin. Essential is the restriction of the oxygen supply to the yeast.
- xylitol is obtained at an amount as high as 78 %.
- the pro ⁇ cess is slowed down at very high initial xylose concentra ⁇ tions.
- the process may, however, be accelerated compared to a normal batch process even by 40 % by using so called fed-batch cultivation, which means that during the pro ⁇ cess, nutrients are added to the fermenter.
- the cultivation was carried out using different xylose concentrations and different aeration efficiences in both a fermenter and in agitated cultures.
- composition of the medium was xylose 40 to 300 g/1 yeast extract 10 g/1
- furfural favourably affected the yield of xylitol.
- the presence of furfural in microbiological processes has been considered purely disadvantageous as furfural inhibits the growth of yeast.
- the inhibition of the growth of the yeast is as such desirable in processes of this kind, as the growth of yeast consumes raw materi ⁇ als which is manifested in a decrease of the amount of xy ⁇ litol.
- furfural did not completely inhibit the action of the yeast, but acted as a factor promoting the production of xylitol.
- furfural usually disturbs both the growth and the fermentation process (for example N. Banerjee et al., European J. Appl. Microbiol. Biotechnol. 1981, 11:226-228)) .
- furfural for the promotion of microbiological processes is not limited to the production of xylitol from xylose by means of the process described above. It is evi ⁇ dent that this observation may be applied also to other microbiological processes, the object of which is not the production of the microbe as such but the production of substances independent of the microbial growth, for examp ⁇ le in biotransformations.
- the yeast concentration was deter ⁇ mined by measuring the turbidity, the xylose concentration by determining the concentration of reducing substances and the formation of xylitol was determined gualitatively by using thin layer chromatography and quantitatively by using liquid chromatography or an enzymatic method.
- the process according to the invention exhibits many ad ⁇ vantages with respect to the known processes. Of these may be mentioned for example: - High xylitol yield, 50 to 78 %, depending on the xylo ⁇ se concentration.
- the process mentioned earlier and be ⁇ longing to the state of art gave a xylitol yield of on ⁇ ly 40.3 % although the initial xylose concentration was only 10 %.
- the yield obtained was 74 %.
- the xylitol produced with this method is pure and is thus quite suitable for use in foodstuffs.
- Xylitol pro ⁇ may contain residues of toxic catalyst.
- the method according to the invention is also more economical, as the catalysts are expensive and are consumed in the process.
- the strain Candida guilliermondii VTT-C-71006 is grown aseptically while agitating in a solution volume of 100 ml in a 250 ml vessel.
- the growth temperature is 28°C.
- the medium contains 80 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone.
- the pH of the medium was adjusted to a value of 5.0.
- the xylose was determined as reducing sugars and the xyli ⁇ tol using liquid chromatograph . The xylitol yield was thus 62.5 %.
- Example 2 The method according to the Example 1 was repeated, the initial xylose concentration being 300 g/1. After 6 days the xylose was consumed completely and the xylitol con ⁇ centration was 150 g/1, which corresponds to a yield of 50 %.
- Example 5 The strain mentioned in the Example 1 is grown aseptically in a fermenter in a medium containing 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. The pH of the medium was adjusted to a value of 5.0 and the stirring speed to 400 rp and the aeration to about 0.5 1/min. Af ⁇ ter 50 hours of cultivation the xylose was totally consu ⁇ med and the xylitol concentration was 74 g/1 (yield 74 %) .
- Example 5 Example 5
- Example 4 The process of Example 4 was repeated but using 250 g/1 as the initial xylose concentration. After cultivating for 160 hours, the xylose was completely consumed and the xy ⁇ litol concentration was 138 g/1 (yield 55 %) .
- the strain mentioned in the previous example is grown in a fermenter as a fed-batch-culture.
- the initial volume of the culture solution was 60 % of the end volume and its composition was 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. Cultivation was started as in the Example 4, but when the xylose concentration had decreased to a value of 40 g/1, the introduction of a xylose solut ⁇ ion at a concentration of 350 g/1 was begun. By means of the addition the xylose concentration in the fermenter was kept at 40 g/1. When the final volume was reached the ad ⁇ dition was stopped. After cultivating for 120 hours the xylose was completely consumed and the xylitol concentrat ⁇ ion was 164 g/1, which corresponds to a xylitol yield of
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI870147A FI76377C (sv) | 1987-01-15 | 1987-01-15 | Mikrobiologisk framställningsmetod |
FI870147 | 1987-01-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1988005467A1 true WO1988005467A1 (en) | 1988-07-28 |
Family
ID=8523778
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FI1987/000162 WO1988005467A1 (en) | 1987-01-15 | 1987-12-08 | Process for the preparation of xylitol from xylose by cultivating candida guilliermondii |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0344163A1 (sv) |
JP (1) | JPH02502333A (sv) |
FI (1) | FI76377C (sv) |
WO (1) | WO1988005467A1 (sv) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2641545A1 (fr) * | 1989-01-10 | 1990-07-13 | Agrocinq | Procede de biosynthese du xylitol |
WO1990008193A1 (en) * | 1989-01-17 | 1990-07-26 | Suomen Xyrofin Oy | Method for the production of xylitol from mixtures containing xylose |
FR2678637A1 (fr) * | 1991-07-01 | 1993-01-08 | Xyrofin Oy | Nouvelles souches de levure pour la production de xylitol. |
FR2720406A1 (fr) * | 1994-05-27 | 1995-12-01 | Agronomique Inst Nat Rech | Composition de microorganismes et procédé pour la production de xylitol. |
US5631150A (en) * | 1992-11-05 | 1997-05-20 | Xyrofin Oy | Manufacturing of xylitol using recombinant microbial hosts |
US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
US6846657B2 (en) | 1990-01-15 | 2005-01-25 | Xyrofin Oy | Process for the simultaneous production of xylitol and ethanol |
US6911565B2 (en) | 2001-09-26 | 2005-06-28 | Danico Sweetners Oy | Process for the production of xylitol |
US7109005B2 (en) | 1990-01-15 | 2006-09-19 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
US10435721B2 (en) | 2016-12-21 | 2019-10-08 | Creatus Biosciences Inc. | Xylitol producing metschnikowia species |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010104361A (ja) * | 2008-10-02 | 2010-05-13 | Musashino Chemical Laboratory Ltd | リグノセルロース系バイオマスを用いた糖化液の製造方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3619369A (en) * | 1968-10-31 | 1971-11-09 | Noda Inst For Scientific Res | Process for producing xylitol by fermentation |
-
1987
- 1987-01-15 FI FI870147A patent/FI76377C/sv not_active IP Right Cessation
- 1987-12-08 JP JP63500556A patent/JPH02502333A/ja active Pending
- 1987-12-08 WO PCT/FI1987/000162 patent/WO1988005467A1/en unknown
- 1987-12-08 EP EP88900367A patent/EP0344163A1/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3619369A (en) * | 1968-10-31 | 1971-11-09 | Noda Inst For Scientific Res | Process for producing xylitol by fermentation |
Non-Patent Citations (4)
Title |
---|
Agr. Biol. Chem. Vol. 30, 1966 Hiroshi Onishi, "The Production of Xylitol. L-Arabinitol and Ribitol by Yeasts", p 1139-1144 * |
CHEMICAL ABSTRACTS, Vol. 102 (1985), Abstract No 102:3048g, Mikrobiologiya. 1984, 53(5), 803-8 (Russ). * |
CHEMICAL ABSTRACTS, Vol. 72 (1970), Abstract No 41685g, Appl. Microbiol. 1969, 18(6), 1031-5 (Eng). * |
CHEMICAL ABSTRACTS, Vol. 94 (1981), Abstract No 94:207106a, Biotechnol. Lett. 1981, 3(3), 125-3 (Eng). * |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2641545A1 (fr) * | 1989-01-10 | 1990-07-13 | Agrocinq | Procede de biosynthese du xylitol |
WO1990008193A1 (en) * | 1989-01-17 | 1990-07-26 | Suomen Xyrofin Oy | Method for the production of xylitol from mixtures containing xylose |
US7109005B2 (en) | 1990-01-15 | 2006-09-19 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
US7625728B2 (en) | 1990-01-15 | 2009-12-01 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
US6846657B2 (en) | 1990-01-15 | 2005-01-25 | Xyrofin Oy | Process for the simultaneous production of xylitol and ethanol |
FR2678637A1 (fr) * | 1991-07-01 | 1993-01-08 | Xyrofin Oy | Nouvelles souches de levure pour la production de xylitol. |
US6271007B1 (en) | 1991-07-01 | 2001-08-07 | Xyrofin Oy | Yeast strains for the production of xylitol |
US5631150A (en) * | 1992-11-05 | 1997-05-20 | Xyrofin Oy | Manufacturing of xylitol using recombinant microbial hosts |
US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
FR2720406A1 (fr) * | 1994-05-27 | 1995-12-01 | Agronomique Inst Nat Rech | Composition de microorganismes et procédé pour la production de xylitol. |
WO1995033063A1 (fr) * | 1994-05-27 | 1995-12-07 | Institut National De La Recherche Agronomique | Composition de microorganismes et procede pour la production de xylitol |
US6911565B2 (en) | 2001-09-26 | 2005-06-28 | Danico Sweetners Oy | Process for the production of xylitol |
US10435721B2 (en) | 2016-12-21 | 2019-10-08 | Creatus Biosciences Inc. | Xylitol producing metschnikowia species |
US11473110B2 (en) | 2016-12-21 | 2022-10-18 | Creatus Biosciences Inc. | Xylitol producing Metschnikowia species |
Also Published As
Publication number | Publication date |
---|---|
FI76377C (sv) | 1989-05-10 |
EP0344163A1 (en) | 1989-12-06 |
FI870147A0 (fi) | 1987-01-15 |
JPH02502333A (ja) | 1990-08-02 |
FI76377B (fi) | 1988-06-30 |
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