WO1988005467A1 - Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii - Google Patents

Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii Download PDF

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Publication number
WO1988005467A1
WO1988005467A1 PCT/FI1987/000162 FI8700162W WO8805467A1 WO 1988005467 A1 WO1988005467 A1 WO 1988005467A1 FI 8700162 W FI8700162 W FI 8700162W WO 8805467 A1 WO8805467 A1 WO 8805467A1
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WIPO (PCT)
Prior art keywords
xylose
xylitol
medium
yeast
preparation
Prior art date
Application number
PCT/FI1987/000162
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English (en)
Inventor
Heikki Ojamo
Lasse Ylinen
Matti Linko
Original Assignee
Farmos-Yhtymä Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Farmos-Yhtymä Oy filed Critical Farmos-Yhtymä Oy
Publication of WO1988005467A1 publication Critical patent/WO1988005467A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida

Definitions

  • the object of the present invention is a microbiological process for the preparation of xylitol from xylose con ⁇ taining mixtures using the yeast strain Candida guillier ⁇ mondii VTT-C-71006.
  • Xylitol is conventionally prepared on a technical scale by hydrolysing hemicellulose containing substances, for example birch wood, whereby a pentose rich hydrolysate is obtained.
  • the xylose of the hydrolysate can thereafter be hydrogenated catalytically to form xylitol.
  • microbiological methods for the production of xylitol are prior known.
  • H. Onishi & T. Suzuki "The Production of Xylitol, L-Arabinitol and Ribitol by Yeasts", Agr. Biol. Chem., Vol. 30, No. 11, p. 1139 to 1144, 1966, is disclosed the microbiological production of xylitol from a xylose containing solution by the yeast species Candida guilliermondii.
  • the initial xylose con ⁇ centration of the solution used as raw material was 10 % by weight.
  • the yield of xylitol was 40.3 %.
  • the strain Candida guilliermondii VTT-C-71006 is deposited in the strain collection at National Collection of Yeast Cultures (NCYC), Norwich, England, on 17th December 1986 under no NCYC-1644.
  • Candida guilliermondii VTT-C-71006 exhibits the following morphological and physiological properties.
  • cultivation is carried out by cultivating the strain Candida guilliermon- dii VTT-C-71006 preferably under aerated conditions in a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
  • a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
  • Suitable raw materials are any xylose containing mixtures, for example the xylose rich mixtures obtained from the hydrolysis of hemicellulose containing materials.
  • the fermentation solution as the N-source for the yeast different ammonium salts may be used and as a growth fac ⁇ tor source for example yeast extract, yeast produced in the process or extract of plant origin. Essential is the restriction of the oxygen supply to the yeast.
  • xylitol is obtained at an amount as high as 78 %.
  • the pro ⁇ cess is slowed down at very high initial xylose concentra ⁇ tions.
  • the process may, however, be accelerated compared to a normal batch process even by 40 % by using so called fed-batch cultivation, which means that during the pro ⁇ cess, nutrients are added to the fermenter.
  • the cultivation was carried out using different xylose concentrations and different aeration efficiences in both a fermenter and in agitated cultures.
  • composition of the medium was xylose 40 to 300 g/1 yeast extract 10 g/1
  • furfural favourably affected the yield of xylitol.
  • the presence of furfural in microbiological processes has been considered purely disadvantageous as furfural inhibits the growth of yeast.
  • the inhibition of the growth of the yeast is as such desirable in processes of this kind, as the growth of yeast consumes raw materi ⁇ als which is manifested in a decrease of the amount of xy ⁇ litol.
  • furfural did not completely inhibit the action of the yeast, but acted as a factor promoting the production of xylitol.
  • furfural usually disturbs both the growth and the fermentation process (for example N. Banerjee et al., European J. Appl. Microbiol. Biotechnol. 1981, 11:226-228)) .
  • furfural for the promotion of microbiological processes is not limited to the production of xylitol from xylose by means of the process described above. It is evi ⁇ dent that this observation may be applied also to other microbiological processes, the object of which is not the production of the microbe as such but the production of substances independent of the microbial growth, for examp ⁇ le in biotransformations.
  • the yeast concentration was deter ⁇ mined by measuring the turbidity, the xylose concentration by determining the concentration of reducing substances and the formation of xylitol was determined gualitatively by using thin layer chromatography and quantitatively by using liquid chromatography or an enzymatic method.
  • the process according to the invention exhibits many ad ⁇ vantages with respect to the known processes. Of these may be mentioned for example: - High xylitol yield, 50 to 78 %, depending on the xylo ⁇ se concentration.
  • the process mentioned earlier and be ⁇ longing to the state of art gave a xylitol yield of on ⁇ ly 40.3 % although the initial xylose concentration was only 10 %.
  • the yield obtained was 74 %.
  • the xylitol produced with this method is pure and is thus quite suitable for use in foodstuffs.
  • Xylitol pro ⁇ may contain residues of toxic catalyst.
  • the method according to the invention is also more economical, as the catalysts are expensive and are consumed in the process.
  • the strain Candida guilliermondii VTT-C-71006 is grown aseptically while agitating in a solution volume of 100 ml in a 250 ml vessel.
  • the growth temperature is 28°C.
  • the medium contains 80 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone.
  • the pH of the medium was adjusted to a value of 5.0.
  • the xylose was determined as reducing sugars and the xyli ⁇ tol using liquid chromatograph . The xylitol yield was thus 62.5 %.
  • Example 2 The method according to the Example 1 was repeated, the initial xylose concentration being 300 g/1. After 6 days the xylose was consumed completely and the xylitol con ⁇ centration was 150 g/1, which corresponds to a yield of 50 %.
  • Example 5 The strain mentioned in the Example 1 is grown aseptically in a fermenter in a medium containing 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. The pH of the medium was adjusted to a value of 5.0 and the stirring speed to 400 rp and the aeration to about 0.5 1/min. Af ⁇ ter 50 hours of cultivation the xylose was totally consu ⁇ med and the xylitol concentration was 74 g/1 (yield 74 %) .
  • Example 5 Example 5
  • Example 4 The process of Example 4 was repeated but using 250 g/1 as the initial xylose concentration. After cultivating for 160 hours, the xylose was completely consumed and the xy ⁇ litol concentration was 138 g/1 (yield 55 %) .
  • the strain mentioned in the previous example is grown in a fermenter as a fed-batch-culture.
  • the initial volume of the culture solution was 60 % of the end volume and its composition was 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. Cultivation was started as in the Example 4, but when the xylose concentration had decreased to a value of 40 g/1, the introduction of a xylose solut ⁇ ion at a concentration of 350 g/1 was begun. By means of the addition the xylose concentration in the fermenter was kept at 40 g/1. When the final volume was reached the ad ⁇ dition was stopped. After cultivating for 120 hours the xylose was completely consumed and the xylitol concentrat ⁇ ion was 164 g/1, which corresponds to a xylitol yield of

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé microbiologique de préparation de xylitol à partir de mélanges contenant du xylose. La souche de levure Candida guilliermondii VTT-C-71006 est cultivée, de préférence dans des conditions d'aération limitée, dans un milieu riche en xylose. On obtient une excellente production de xylitol en ajoutant au milieu du furfural en une quantité comprise entre environ 0,2 et 1,0 g/l. Pendant la fermentation, on utilise soit un procédé par lots conventionnel soit un procédé par lots à alimentation.
PCT/FI1987/000162 1987-01-15 1987-12-08 Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii WO1988005467A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI870147A FI76377C (fi) 1987-01-15 1987-01-15 Mikrobiologisk framstaellningsmetod.
FI870147 1987-01-15

Publications (1)

Publication Number Publication Date
WO1988005467A1 true WO1988005467A1 (fr) 1988-07-28

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PCT/FI1987/000162 WO1988005467A1 (fr) 1987-01-15 1987-12-08 Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii

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EP (1) EP0344163A1 (fr)
JP (1) JPH02502333A (fr)
FI (1) FI76377C (fr)
WO (1) WO1988005467A1 (fr)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641545A1 (fr) * 1989-01-10 1990-07-13 Agrocinq Procede de biosynthese du xylitol
WO1990008193A1 (fr) * 1989-01-17 1990-07-26 Suomen Xyrofin Oy Methode de production de xylitol a partir de melanges contenant du xylose
FR2678637A1 (fr) * 1991-07-01 1993-01-08 Xyrofin Oy Nouvelles souches de levure pour la production de xylitol.
FR2720406A1 (fr) * 1994-05-27 1995-12-01 Agronomique Inst Nat Rech Composition de microorganismes et procédé pour la production de xylitol.
US5631150A (en) * 1992-11-05 1997-05-20 Xyrofin Oy Manufacturing of xylitol using recombinant microbial hosts
US6723540B1 (en) 1992-11-05 2004-04-20 Xyrofin Oy Manufacture of xylitol using recombinant microbial hosts
US6846657B2 (en) 1990-01-15 2005-01-25 Xyrofin Oy Process for the simultaneous production of xylitol and ethanol
US6911565B2 (en) 2001-09-26 2005-06-28 Danico Sweetners Oy Process for the production of xylitol
US7109005B2 (en) 1990-01-15 2006-09-19 Danisco Sweeteners Oy Process for the simultaneous production of xylitol and ethanol
US7226761B2 (en) 1992-11-05 2007-06-05 Danisco Sweeteners Oy Manufacture of five-carbon sugars and sugar alcohols
US10435721B2 (en) 2016-12-21 2019-10-08 Creatus Biosciences Inc. Xylitol producing metschnikowia species

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010104361A (ja) * 2008-10-02 2010-05-13 Musashino Chemical Laboratory Ltd リグノセルロース系バイオマスを用いた糖化液の製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3619369A (en) * 1968-10-31 1971-11-09 Noda Inst For Scientific Res Process for producing xylitol by fermentation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3619369A (en) * 1968-10-31 1971-11-09 Noda Inst For Scientific Res Process for producing xylitol by fermentation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Agr. Biol. Chem. Vol. 30, 1966 Hiroshi Onishi, "The Production of Xylitol. L-Arabinitol and Ribitol by Yeasts", p 1139-1144 *
CHEMICAL ABSTRACTS, Vol. 102 (1985), Abstract No 102:3048g, Mikrobiologiya. 1984, 53(5), 803-8 (Russ). *
CHEMICAL ABSTRACTS, Vol. 72 (1970), Abstract No 41685g, Appl. Microbiol. 1969, 18(6), 1031-5 (Eng). *
CHEMICAL ABSTRACTS, Vol. 94 (1981), Abstract No 94:207106a, Biotechnol. Lett. 1981, 3(3), 125-3 (Eng). *

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641545A1 (fr) * 1989-01-10 1990-07-13 Agrocinq Procede de biosynthese du xylitol
WO1990008193A1 (fr) * 1989-01-17 1990-07-26 Suomen Xyrofin Oy Methode de production de xylitol a partir de melanges contenant du xylose
US7109005B2 (en) 1990-01-15 2006-09-19 Danisco Sweeteners Oy Process for the simultaneous production of xylitol and ethanol
US7625728B2 (en) 1990-01-15 2009-12-01 Danisco Sweeteners Oy Process for the simultaneous production of xylitol and ethanol
US6846657B2 (en) 1990-01-15 2005-01-25 Xyrofin Oy Process for the simultaneous production of xylitol and ethanol
FR2678637A1 (fr) * 1991-07-01 1993-01-08 Xyrofin Oy Nouvelles souches de levure pour la production de xylitol.
US6271007B1 (en) 1991-07-01 2001-08-07 Xyrofin Oy Yeast strains for the production of xylitol
US5631150A (en) * 1992-11-05 1997-05-20 Xyrofin Oy Manufacturing of xylitol using recombinant microbial hosts
US6723540B1 (en) 1992-11-05 2004-04-20 Xyrofin Oy Manufacture of xylitol using recombinant microbial hosts
US7226761B2 (en) 1992-11-05 2007-06-05 Danisco Sweeteners Oy Manufacture of five-carbon sugars and sugar alcohols
FR2720406A1 (fr) * 1994-05-27 1995-12-01 Agronomique Inst Nat Rech Composition de microorganismes et procédé pour la production de xylitol.
WO1995033063A1 (fr) * 1994-05-27 1995-12-07 Institut National De La Recherche Agronomique Composition de microorganismes et procede pour la production de xylitol
US6911565B2 (en) 2001-09-26 2005-06-28 Danico Sweetners Oy Process for the production of xylitol
US10435721B2 (en) 2016-12-21 2019-10-08 Creatus Biosciences Inc. Xylitol producing metschnikowia species
US11473110B2 (en) 2016-12-21 2022-10-18 Creatus Biosciences Inc. Xylitol producing Metschnikowia species

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Publication number Publication date
JPH02502333A (ja) 1990-08-02
EP0344163A1 (fr) 1989-12-06
FI76377C (fi) 1989-05-10
FI870147A0 (fi) 1987-01-15
FI76377B (fi) 1988-06-30

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