Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
  • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
  • C07D211/10—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with radicals containing only carbon and hydrogen atoms attached to ring carbon atoms
  • C07D211/14—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with radicals containing only carbon and hydrogen atoms attached to ring carbon atoms with hydrocarbon or substituted hydrocarbon radicals attached to the ring nitrogen atom
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
  • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
  • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
  • C07D211/20—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms
  • C07D211/22—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms by oxygen atoms
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
  • A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/04—Centrally acting analgesics, e.g. opioids
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
  • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
  • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
  • C07D211/30—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by doubly bound oxygen or sulfur atoms or by two oxygen or sulfur atoms singly bound to the same carbon atom
  • C07D211/32—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by doubly bound oxygen or sulfur atoms or by two oxygen or sulfur atoms singly bound to the same carbon atom by oxygen atoms

Definitions

• the present invention relates to 1,4-disubstituted-piperidinyl compounds which are useful as analgesics and muscle relaxants. Another aspect of the invention relates to methods for relieving pain. A further aspect of the present invention relates to methods for relieving muscle spasms.
• each R and R' is independently selected from the group consisting of lower alkyl, lower alkoxy, hydrogen and halogen;
• X is represented by either a carbonyl group or a hydroxymethylene group;
• A' is at least one substituent selected from the group consisting of halogen, lower alkyl, and lower alkoxy; and, A is selected from the group consisting of halogen, hydrogen, lower alkyl, and lower alkoxy; with the proviso that when X is a carbonyl group, A' is not a halogen when A is a lower alkyl or lower alkoxy.
• X is represented by a carbonyl group or a hydroxymethylene group; and A is selected from the group consisting of halogen, hydrogen, lower alkyl, and lower alkoxy; and,
• each B and B' are independently selected from the group consisting of halogen, lower alkyl, lower alkoxy, and hydrogen; with the proviso that B' and B are not both halogens.
• Y is represented by a hydroxymethylene group or a carbonyl group
• Z is represented by a hydroxymethylene group or a carbonyl group
• each D and D' are independently selected from the group consisting of lower alkyl, lower alkoxy, halogen and hydrogen; with the following provisos: i) that when Y and Z are both represented by carbonyl groups, then D is not a lower alkyl or lower alkoxy when D' is a halogen; and, ii) that when Y is represented by a carbonyl group and Z is represented by a hydroxymethylene group, then D and D' are not both halogens.
• hydroxymethylene group refers to a structure corresponding to, --CHOH--;
• carbonyl group refers to a structure corresponding to --CO--
• halogen refers to a fluorine, chlorine or bromine atom
• lower alkyl group refers to a branched or straight chain alkyl group containing from 1-4 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl and isobutyl; and,
• lower alkoxy group refers to a straight or branched alkoxy group containing from 1-4 carbon atoms, such as methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy and isobutoxy.
• pharmaceutically acceptable acid addition salts is intended to apply to any non-toxic organic or inorganic acid addition salt of the base compounds represented by Formulae I-V.
• inorganic acids which form suitable salts include hydrochloric, hydrobromic, sulphuric and phosphoric acid and acid metal salts such as sodium monohydrogen orthophosphate and potassium hydrogen sulfate.
• organic acids which form suitable salts include the mono-, di- and tricarboxylic acids.
• Such acids are, for example, acetic, glycolic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, malic, tartaric, citric, ascorbic, maleic, hydroxymaleic, benzoic, hydroxybenzoic, phenylacetic, cinnamic, salicylic, 2-phenoxybenzoic and sulfonic acids such as methanesulfonic acid, p-toluenesulfonic acid and 2-hydroxyethane sulfonic acid.
• Some of the compounds of Formulae I-V contain asymmetric centers. Any reference in this application to one of the compounds represented by Formulae I-V is meant to encompass either a specific optical isomer or a mixture of enantiomers or diasteriomers.
• the specific optical isomers can be separated and recovered by techniques known in the art such as chromatography on chiral stationary phases or resolution via chiral salt formation and subsequent separation by selective crystallization.
• the specific optical isomers can also be resolved by the use of enantiospecific enzymes as is known in the art.
• the alcohol is acylated with an acylating agent such as acetyl chloride, butyryl chloride, chloroacetyl chloride, or their equivalents.
• the compound containing the acylated alcohol is then contacted with a suitable enantiospecific enzyme thereby producing the desired stereoisomerically enriched alcohol via enzymatic hydrolysis.
• the desired alcohol can be separated from the complementary stereoisomerically enriched acylated alcohol by-product by chromatographic techniques as is known in the art.
• Such enzymes include the fungal lipase Amano AP-12®, derived from Asperqillus niqer and the fungal lipase derived from Candida cylindracea.
• the acylation is typically conducted in an organic solvent such as DMF.
• the enzymatic hydrolysis is typically conducted in an aqueous buffer with or without an organic solvent such as hexane, heptane, chloroform, ethyl ether and dichloromethane.
• the quantity of enzyme can vary widely as known to those skilled in the art.
• An alternative method of resolving those compounds of Formulae I-V which contain an alcohol is by enzymatic esterification.
• the racemic alcohol containing compound of Formulae I-V is contacted with a suitable enantiospecific enzyme, such as one of those described above, in the presence of an esterifying agent such butyric acid, palmytic acid, lauric acid, ethyl acetate, or dichloroethyl butyrate.
• the desired stereoisomerically enriched ester and the complementary stereoisomerically enriched alcohol by-product can be separated by conventional chromatographic techniques. After the desired ester is separated, it can be subjected to a basic hydrolysis to produce the desired compound.
• the enzymatic esterification is also typically conducted in an organic solvent such as one of those described above.
• Other enantiospecific enzymes are known in the art. Some of these may also be suitable for either of the resolution techniques discussed above as known to those skilled in the art.
• Suitable analgesics and muscle relaxants represented by Formulae I-V are those illustrated below:
• Each of the phenyl rings contained within the compounds represented by Formulae I-V may be substituted at up to three positions with the indicated chemical entities. These substitutions may be located at positions 2-6 of the phenyl ring. Each phenyl ring may be substituted with differing chemical entities or a single chemical entity. Thus, for example, in Formula V, D can represent up to 3 substituents which may be the same or differing chemical entities which are located at any of positions 2-6 on the indicated ring. It is also possible for the two phenyl rings contained within a compound to have differing substituents provided that the limitations of the provisos described above are followed.
• the more preferred compounds of the present invention are those of Formula I.
• the most preferred compounds of the present invention are those selected from the group consisting of:
• the compounds of the present invention can be synthesized by techniques known in the art. A currently preferred and novel manner of preparing these compounds can be described by the following processes.
• the desired compound is a 1,4-disubstituted-piperidinyl diketone or a 1-(optionally substituted)-phenacyl-4-hydroxyarylmethyl-piperidine as described by Formulae II or III then the following synthesis can be utilized.
• the 4-substituted-piperidine (compound of Formula VI) should correspond structurally to its counterpart in the desired 1,4-disubstituted-piperidinyl compound since all of the substituents on the 4-substituted-piperidine will be retained in the final product.
• X in the desired 1,4-disubstituted-piperidinyl compound is a carbonyl group
• the 4-substituted-piperidine of Formula VI utilized as a starting material should be substituted with a carbonyl group at the 4 position of the piperidinyl ring.
• the 4-substituted-piperidine of Formula VI used as a starting material should be substituted with a hydroxymethylene group at the 4 position of the piperidinyl ring.
• a in the desired 1,4-disubstituted-piperidinyl compound is a 4-fluorosubstituent, then the 4-substituted-piperidine should contain a fluorine at the 4-position of the indicated phenyl ring.
• the optionally-substituted-phenacyl halide (compound of Formula VII) should correspond structurally to its counterpart in the desired 1,4-disubstituted-piperidinyl compound since all of its substituents with the exception of the halogen atom represented by Y, will be retained in the final product.
• A' is a 4-fluoro substituent
• the optionally-substituted-phenacyl halide should contain a fluorine at the 4-position of the indicated phenyl ring.
• 1,4-disubstituted-piperidinyl compound is 2-[4-(4-fluorobenzoyl)-1-piperidinyl]-1-(4-fluorophenyl)-ethanone
• the preferred starting materials are: a) 4-fluorophenyl-4-piperidinyl ketone; and, b) 2-chloro-4'-fluoroacetophenone.
• reaction be conducted in the presence of either an organic or inorganic base.
• Sodium bicarbonate is currently utilized.
• the base is preferably present in a molar excess relative to the 4-substituted-piperidine.
• reaction be conducted in the presence of an alkali-iodo catalyst.
• Sodium iodide is currently utilized.
• the alkali-iodo catalyst is generally present in a quantity of from 0.1 to 1 mol percent based upon the quantity of 4-substituted-piperidine present in the reaction zone.
• the 4-substituted-piperidine and the optionally-substituted-phenacyl halide are generally stirred together for a period of time ranging from 1 to 30 hours. It is preferred that the reaction be conducted at a temperature range of from 25° to 115° C. It is also preferred that the reaction be conducted in an organic solvent.
• suitable solvents include dichloromethane, methanol, tetrahydrofuran, toluene, chloroform, and the like.
• the 1,4-disubstituted-piperidinyl compound produced above can be recovered from the reaction zone by techniques known in the art.
• One suitable technique is to extract the reaction zone with an organic solvent after water has been added to the reaction.
• the desired 1,4-disubstituted-piperidinyl compound will be found in the organic phase.
• the 1,4-disubstituted-piperidinyl compound can then be purified by techniques known in the art.
• One such suitable technique is recrystallization from a suitable solvent system.
• suitable solvent systems include methanol/butanone and methanol/ethyl acetate if the desired compound is present as an acid addition salt.
• Ethyl acetate/hexane and chloroform/ benzene are examples of suitable solvent systems if the desired compound is present as a free base.
• Other solvent systems known to those skilled in the art could also be utilized.
• the desired compound is a 1,4-disubstituted-piperidinyl diol as described by Formula I, then one method of preparing the compound is the following.
• the starting material is a 1,4-disubstituted-piperidinyl diketone that can be described by the following formula VIII ##STR5## wherein R and R' are as defined in Formula I.
• the desired 1,4-disubstituted-piperidinyl diol can then be obtained by reducing the piperidinyl diketone of Formula VIII with an appropriate reducing agent.
• the 1,4-disubstituted-piperidinyl diketone (as described by Formula VIII) which is utilized in the reduction should correspond structurally to the desired 1,4-disubstituted-piperidinyl diol, since all of the diketones substituents (with the exception of the carbonyls) will be retained in the final product.
• R' in the desired diol is a 4-fluoro-substituent, then the diketone utilized should be substituted with a fluorine atom at the 4-position of the indicated phenyl ring.
• ⁇ -(4-fluorophenyl)-4-[(4-fluorophenyl) hydroxymethyl]-1-piperidineethanol can be obtained by the reduction of 2-[4-(4-fluorobenzoyl)-1-piperidinyl]-1-(4-fluorophenyl)-ethanone.
• the diketone starting material of Formula VIII can be obtained in the manner previously taught for preparing the diketone compounds of Formula II and III.
• the reduction of the 1,4-disubstituted-piperidinyl diketone may be carried out with a variety of reducing agents as known to those skilled in the art.
• Lithium aluminum hydride and sodium borohydride are representative examples of suitable reducing agents.
• Sodium borohydride is currently preferred.
• sodium borohydride is utilized as the reducing agent, it is preferably present in the reaction zone in a molar excess relative to the the quantity of 1,4-disubstituted-piperidinyl diketone present and more preferably, is present in the molar ratio of from 2-4 moles of reducing agent for every mole of 1,4-disubstituted-piperidinyl diketone utilized.
• the reduction be conducted at a temperature range of from 0° to 25° C. and for a period of time ranging from 1-24 hours. It is also preferred that the reaction be conducted in an organic solvent.
• suitable solvents include tetrahydrofuran and ether. Methanol is suitable for use with sodium borohydride.
• reaction be quenched by the addition of water.
• the 1,4-disubstituted-piperidinyl diol may then be recovered from the reaction zone by numerous techniques as known to those skilled in the art.
• One suitable technique is to extract the reaction zone with an organic solvent after the addition of water.
• the 1,4-disubstituted-piperidinyl diol can then be purified by techniques known in the art.
• One such suitable technique is recrystallization from a suitable solvent system.
• suitable solvent systems include methanol/butanone and methanol/ethyl acetate if the desired compound is present as an acid addition salt.
• Ethyl acetate/hexane and chloroform/benzene are examples of suitable solvent systems if the desired compound is present as a free base.
• Other solvent systems known to those skilled in the art could also be utilized.
• the 1,4-disubstituted-piperidinyl diol can be produced by reducing a 1-(optionally substituted)-phenacyl-4-hydroxyarylmethyl-piperidine as described in Formula II and III, that is structurally analogous to the desired piperidinyl diol.
• the reduction can be conducted in the same manner as that described above.
• 1,4-disubstituted-piperidinyl diol can be produced by reducing an ⁇ -aryl-4-aroyl-piperidineethanol as described by Formula IV that is structurally analogous to the desired piperidinyl diol. The reduction should be conducted in a manner analogous to that described above.
• the 4-hydroxymethyl-substituted piperidine (compound of Formula IX) should be structurally analogous to its counterpart in the desired 1,4-disubstituted-piperidinyl diol since all of its substituents will be retained in the piperidinyl diol.
• the optionally-substituted-styrene oxide (compound of Formula X) should be structurally analogous to its counterpart in the desired 1,4-disubstituted-piperidinyl diol since all of its substituents will be retained in the piperidinyl diol.
• 4-(hydroxyphenylmethyl)- ⁇ -phenyl-1-piperidineethanol can be prepared utilizing: i) 4-hydroxyphenylmethyl piperidine; and, ii) styrene oxide.
• the optionally-substituted-styrene oxide and the 4-hydroxymethyl-substituted piperidine be present in the reaction zone in approximately equimolar quantities, although a slight excess of either reactant is not deleterious. They are generally reacted together for a period of time ranging from 1-24 hours. It is also preferred that they be reacted at a temperature range of from 25°-115° C.
• the reaction is generally conducted in an organic solvent.
• suitable solvents include tetrahydrofuran, toluene, chloroform, dichloromethane and the like.
• the desired 1,4-disubstituted-piperidinyl diol can be recovered from the reaction zone by techniques known in the art.
• the diols are currently recovered from the reaction by subjecting the diol-containing solvent to evaporation on a rotary evaporator.
• the 1,4-disubstituted-piperidinyl diol can then be purified by techniques known in the art.
• One such suitable technique is recrystallization from a suitable solvent system.
• suitable solvent systems include methanol/butanone and methanol/ethyl acetate if the desired compound is present as an acid addition salt.
• Ethyl acetate/hexane and chloroform/benzene are examples of suitable solvent systems if the desired compound is present as a free base.
• Other solvent systems known to those skilled in the art could also be utilized.
• ⁇ -aryl-4-aroyl-piperidineethanol of Formula IV can be synthesized by techniques known in the art. The following novel synthetic method is preferred.
• the starting materials are a 4-aroyl-substituted-piperidine as described by Formula XI and an optionally-substituted-styrene oxide as described by Formula XII ##STR7## wherein B and B' are as described in Formula IV.
• the 4-aroyl-substitued piperidine should correspond structurally to its counterpart in the desired ⁇ -aryl-4-aroyl-piperidineethanol since all of its substituents will be retained in the final structure.
• the optionally-substituted-styrene oxide should also correspond structurally to its counterpart in the ⁇ -aryl-4-aroyl-piperidineethanol since all of its substituents will be retained in the final structure.
• the starting materials are: i) 3,4-dimethoxyphenyl-4-piperidinyl ketone; and, ii) 4-fluorostyrene oxide.
• approximately equimolar concentrations of the 4-aroyl-substituted-piperidine and styrene oxide are reacted together for a period of time ranging from 1-24 hours. A slight excess of either reactant is acceptable. It is also preferred that the reaction be conducted at a temperature of from 25°-115° C.
• the reaction is generally conducted in an organic solvent.
• suitable solvents include tetrahydrofuran, toluene, chloroform, dichloromethane and the like.
• the desired ⁇ -aryl-4-aroyl-piperidineethanol can be recovered from the reaction zone by numerous techniques as known in the art.
• One suitable technique is chromatography utilizing a silica gel adsorbent and an ethyl acetate eluent.
• the desired ⁇ -aryl-4-aroyl-piperidineethanol can then be purified by techniques known in the art.
• One such suitable technique is recrystallization from a suitable solvent system.
• suitable solvent systems include methanol/butanone and methanol/ethyl acetate if the desired compound is present as an acid addition salt.
• Ethyl acetate/hexane and chloroform/benzene are examples of suitable solvent systems if the desired compound is present as a free base.
• Other solvent systems known to those skilled in the art could also be utilized.
• the compounds described by Formula V may be utilized as analgesics.
• the compounds possess a level of potency sufficient to inhibit the sensation of the severe levels of pain that are commonly associated with conditions such as metastatic carcinoma, myocardial infarctions or traumatic injuries.
• the compounds are non-narcotic. This means that they are devoid of the abuse potential that accompanies most analgesics.
• mice should be administered from 0.1 to 200 mg/kg of the compound either subcutaneously or intragastrically. Thirty minutes after the administration of the test compound, the mice should be administered 0.4 ml of a 0.25% v/v solution of acetic acid intraperitoneally.
• mice Five minutes after the administration of the acetic acid, the mice should be observed for signs of squirming and writhing which is indicative of pain.
• a compound is considered to posses significant analgesic activity if the mice which are administered said compound do not exhibit signs of pain during the test (i.e., squirming and writhing).
• mice should be administered up to 800 mg/kg of the desired compound subcutaneously. Thirty minutes later the mice should be placed upon a hot plate which has been heated to a temperature of 55° C.
• a compound is considered to be non-narcotic if the mice jump within the first 20 seconds of when they are initially placed upon the hot plate.
• mice From 5 to 10 mice should be administered from 0.1 to 200 mg/kg of the compound. Thirty minutes later the mice should be administered 60 mg/kg of morphine subcutaneously.
• mice should be observed for 30 minutes after the administration of the morphine in order to determine whether the test compound has blocked the ability of morphine to cause the sustained contraction of the sacrococcygeus dorsalis muscle in the mice. Contraction of this muscle causes the tails of the mice to be elevated at an angle of at least 90°. Thus if a compound is a muscle relaxant, the tails of the mice will not be elevated.
• mice are initially screened for use in the test by placing them on a horizontal rod which is rotating at 15 rpm. Those mice which fall off during a 120 second interval are excluded from further testing.
• mice satisfying the criterion described above are then administered up to 800 mg/kg either subcutaneously or intragastrically of the test compound.
• mice Thirty minutes later the mice are placed back upon the rotating horizontal bar and observed for 90 seconds.
• a compound is considered to be non-sedative and to not impair motor skills, if the ratio between the dose at which approximately one-half of the mice fall off the rotating rod and the dose at which approximately one-half of the mice did not experience morphine induced contraction of the sacrococcygeus dorsalis muscle is about 2:1 or greater.
• the compounds of the present invention may be administered by a variety of routes. They are effective if administered parenterally (i.e. subcutaneously, intravenously, intramuscularly, or intraperitoneally).
• the compounds are administered parenterally.
• the quantity of the compound administered will vary depending on the patient, the mode of administration, and the severity of the condition that is being treated. Repetitive daily administration of the compounds may be desired and will vary with patient condition and mode of administration.
• the dosage required will vary from patient to patient, it is generally preferred that the compounds of the present invention be administered within a dosage range of from 0.1-200 mg/kg of patient body weight/day whether being administered orally or parenterally. This dosage range is applicable whether the compounds are being utilized as an analgesic or as a muscle relaxant.
• the term patient refers to a warm-blooded animal.
• the compounds are effective for the relief of pain and muscle spasms in birds, such as chickens and turkeys, or mammals, such as humans, primates, sheep, horses, cattle, pigs, dogs, cats, rats, and mice, etc.
• the compounds can be formulated into solid or liquid preparations such as capsules, pills, tablets, lozenges, melts, powders, suspensions, or emulsions.
• Solid unit dosage forms can be capsules of the ordinary gelatin type containing, for example, surfactants, lubricants and inert fillers such as lactose, sucrose, and cornstarch or they can be sustained release preparations.
• the compounds of Formulae I-V can be tableted with conventional tablet bases such as lactose, sucrose, and cornstarch in combination with binders, such as acacia, cornstarch, or gelatin, disintegrating agents such as potato starch or algenic acid, and a lubricant such as stearic acid or magnesium stearate.
• Liquid preparations are prepared by dissolving the active ingredient in an aqueous or non-aqueous pharmaceutically acceptable solvent which may also contain suspending agents, sweetening agents, flavoring agents, and preservative agents as are known in the art.
• the compounds may be dissolved in a physiologically acceptable pharmaceutical carrier and administered as either a solution or a suspension.
• suitable pharmaceutical carriers are water, saline, dextrose solutions, fructose solutions, ethanol, or oils of animal, vegetative, or synthetic origin.
• the pharmaceutical carrier may also contain preservatives, buffers, etc., as are known in the art.
• the purpose of this example is to demonstrate one manner of preparing the 1,4-disubstituted-piperidinyl diketone, 2-[4-(4-fluorobenzoyl)-1-piperidinyl]-1-(4-fluorophenyl)-ethanone hydrochloride.
• reaction mixture was then cooled and the organic layer was separated and saved for further purification.
• the aqueous layer was extracted with additional toluene and the organic layer was separated and saved for further purification.
• the purpose of this example is to demonstrate one manner of preparing the 1,4-disubstituted-piperidinyl diol, 2-(4-fluorophenyl)-4-[4-(4-fluorophenyl)hydroxymethyl]-1-piperidineethanol.
• reaction mixture was then diluted with 500 ml of water and stirred for 1.5 hours. Approximately 1500 ml of methanol was removed by rotary evaporation at reduced pressure. The resulting mixture was then extracted with chloroform.
• This oil was dissolved in 500 ml of isopropyl alcohol and cooled to a temperature of approximately 0° C. resulting in the formation of a precipitate.
• the purpose of this example is to demonstrate one manner of preparing the 1,4-disubstituted-piperidinyl diol, ⁇ -(4-fluorophenyl)-4-(hydroxyphenylmethyl)-1-piperidineethanol.
• the solution was concentrated on a rotary evaporator and then diluted with water. This mixture was then extracted with ether. The resulting organic layer was separated and then washed with a solution of 10% aqueous hydrogen chloride. The resulting aqueous layer was basified with sodium carbonate and then extracted with chloroform.
• the resulting organic layer was separated, dried with magnesium sulfate, filtered, and then concentrated on a rotary evaporator.
• the concentrate was treated with methanolic hydrogen chloride and concentrated on a rotary evaporator to yield a solid.
• the solid was purified by recrystallization from methanol/ethyl acetate.
• the purpose of this example is to demonstrate one manner of preparing the 1,4-disubstituted-piperidinyl diol, 4-(hydroxyphenylmethyl)- ⁇ -phenyl-1-piperidineethanol.
• the purpose of this example is to demonstrate a manner of preparing the ⁇ -aryl-4-aroyl-piperidineethanol, (3,4-dimethoxyphenyl)[1-[2-(4-fluorophenyl)-2-hydroxyethyl]-4piperidinyl]-methanone.
• the resulting eluent was concentrated by a rotary evaporator.
• the resulting solid was subjected to recrystallization in ethyl acetate and hexane producing 0.4 g of (3,4-dimethoxyphenyl)[1-[2-(4-fluorophenyl)-2-hydroxyethyl]4-piperidinyl]-methanone which had a melting point of 132°-136° C.
• the purpose of this example is to demonstrate one manner of preparing the 1,4-disubstituted-piperidinyl diketone, 2-(4-benzoyl-1-piperidinyl)-1-phenylethanone hydrochloride.
• the reaction mixture was cooled to 60° C., filtered, and then concentrated on a rotary evaporator to an oil.
• the purpose of this example is to demonstrate one method for preparing the 1-(substituted phenacyl)-4-hydroxyarylmethyl-piperidine, 1-(4-fluorophenyl)-2-[4-[(4-fluorophenyl)hydroxymethyl]-1-piperidinyl]-ethanone p-toluenesulfonate.
• the thick oil was diluted with ether and then treated with p-toulenesulfonic acid resulting in the formation of a precipitate.

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• 1988
  • 1988-12-07 US US07/279,900 patent/US5064838A/en not_active Expired - Fee Related
• 1989
  • 1989-01-16 CA CA000588352A patent/CA1330087C/en not_active Expired - Fee Related
  • 1989-01-17 NZ NZ227643A patent/NZ227643A/xx unknown
  • 1989-01-17 FI FI890245A patent/FI90534C/fi not_active IP Right Cessation
  • 1989-01-18 HU HU90238A patent/HU205073B/hu not_active IP Right Cessation
  • 1989-01-18 HU HU89189A patent/HU202494B/hu not_active IP Right Cessation
  • 1989-01-19 AR AR89313027A patent/AR247385A1/es active
  • 1989-01-20 EP EP89100955A patent/EP0325268B1/en not_active Expired - Lifetime
  • 1989-01-20 NO NO890269A patent/NO174505B/no unknown
  • 1989-01-20 DK DK026389A patent/DK26389A/da not_active Application Discontinuation
  • 1989-01-20 ES ES89100955T patent/ES2058346T3/es not_active Expired - Lifetime
  • 1989-01-20 DE DE8989100955T patent/DE68906801T2/de not_active Expired - Fee Related
  • 1989-01-20 KR KR1019890000597A patent/KR0131377B1/ko not_active IP Right Cessation
  • 1989-01-20 AT AT89100955T patent/ATE90077T1/de not_active IP Right Cessation
  • 1989-01-20 JP JP1013022A patent/JP2967990B2/ja not_active Expired - Fee Related
  • 1989-01-20 PT PT89497A patent/PT89497B/pt not_active IP Right Cessation
  • 1989-01-20 PH PH38074A patent/PH27115A/en unknown
  • 1989-01-20 AU AU28687/89A patent/AU615713B2/en not_active Ceased
  • 1989-01-21 CN CN89100339A patent/CN1035823A/zh active Pending
  • 1989-05-09 AR AR89313868A patent/AR244666A1/es active

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