US20060234377A1 - Cell sheets for ectocornea formation, method of producing the same and method of using the same - Google Patents

Cell sheets for ectocornea formation, method of producing the same and method of using the same Download PDF

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Publication number
US20060234377A1
US20060234377A1 US10/544,541 US54454104A US2006234377A1 US 20060234377 A1 US20060234377 A1 US 20060234377A1 US 54454104 A US54454104 A US 54454104A US 2006234377 A1 US2006234377 A1 US 2006234377A1
Authority
US
United States
Prior art keywords
cell sheet
corneal
cells
corneal epithelium
epithelium forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/544,541
Other languages
English (en)
Inventor
Teruo Okano
Kohji Nishida
Masayuki Yamato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DELLSEED Inc
Cellseed Inc
Original Assignee
Teruo Okano
Kohji Nishida
Masayuki Yamato
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2003068899A external-priority patent/JP3935093B2/ja
Priority claimed from JP2003079100A external-priority patent/JP3935096B2/ja
Application filed by Teruo Okano, Kohji Nishida, Masayuki Yamato filed Critical Teruo Okano
Publication of US20060234377A1 publication Critical patent/US20060234377A1/en
Assigned to NISHIDA, KOHJI, DELLSEED INC. reassignment NISHIDA, KOHJI ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: NISHIDA, KOHJI, OKANO, TERUO, YAMATO, MASAYUKI
Assigned to CELLSEED INC., KOHJI NISHIDA reassignment CELLSEED INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: NISHIDA, KOHJI, OKANO, TERUO, YAMATO, MASAYUKI
Priority to US15/859,304 priority Critical patent/US10434219B2/en
Abandoned legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

Definitions

  • anterior segment related cells that are contemplated in the present invention, such as corneal epithelial cells, corneal endothelial cells and conjunctival epithelial cells, do not have as strong intercellular binding as dermal cells and have had the problem that cultivated cells cannot be detached and recovered as a single sheet even if the dispase is employed.
  • the temperature responsive polymer which is used to cover the substrate of the cell culture support is characterized in that its hydrating power varies in a temperature range of 0-80° C., more preferably 20-50° C.
  • One version of this temperature responsive polymer is such that it has an upper or lower critical dissolution temperature of 0° C.-80° C., more preferably 20° C.-50° C., in aqueous solution. Beyond 80° C., cells may die, which is not preferred. Below 0° C., the cell growth rate will generally drop by an extreme degree or cells will die, which also is not preferred.
  • corneal epithelium forming cell sheet described in the present invention is not limited in any particular way and as already mentioned above, it may be used to treat not only diseased sites where the anterior segment tissue is partly or entirely damaged or deficient as in corneal erosion and corneal ulceration but also binocular refractory conjunctival diseases without corneal epithelial cells.
  • the cells became confluent and were then cultivated for an additional 6 days until they stratified.
  • a carrier molded from a 2.1 cm ⁇ polyvinylidene difluoride (PVDF) membrane having a 1.5 cm ⁇ circular cutout in the center was placed over the cells; the culture medium was gently aspirated through the cutout and subjected to a low-temperature treatment by incubating and cooling at 20° C. for 30 minutes together with the cell culture support material, whereupon the stratified, corneal epithelial cell sheet on the cell culture support material was detached together with the overlying carrier.
  • the detached stratified sheet had adequate strength as a single sheet, with a shrinkage of no more than 5%.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
US10/544,541 2003-02-06 2004-02-06 Cell sheets for ectocornea formation, method of producing the same and method of using the same Abandoned US20060234377A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/859,304 US10434219B2 (en) 2003-02-06 2017-12-29 Method of treatment using corneal epithelium forming cell sheets

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2003068899A JP3935093B2 (ja) 2003-02-06 2003-02-06 高生着性再生角膜上皮細胞シート、製造方法及びその利用方法
JP2003-068899 2003-02-06
JP2003079100A JP3935096B2 (ja) 2003-02-14 2003-02-14 高生着性角膜上皮代替細胞シート、製造方法及びその利用方法
JP2003-079100 2003-02-14
PCT/JP2004/001274 WO2004069295A1 (ja) 2003-02-06 2004-02-06 角膜上皮形成用細胞シート、それらの製造方法及びそれらの利用方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/001274 A-371-Of-International WO2004069295A1 (ja) 2003-02-06 2004-02-06 角膜上皮形成用細胞シート、それらの製造方法及びそれらの利用方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US15/859,304 Division US10434219B2 (en) 2003-02-06 2017-12-29 Method of treatment using corneal epithelium forming cell sheets

Publications (1)

Publication Number Publication Date
US20060234377A1 true US20060234377A1 (en) 2006-10-19

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US10/544,541 Abandoned US20060234377A1 (en) 2003-02-06 2004-02-06 Cell sheets for ectocornea formation, method of producing the same and method of using the same
US15/859,304 Expired - Lifetime US10434219B2 (en) 2003-02-06 2017-12-29 Method of treatment using corneal epithelium forming cell sheets

Family Applications After (1)

Application Number Title Priority Date Filing Date
US15/859,304 Expired - Lifetime US10434219B2 (en) 2003-02-06 2017-12-29 Method of treatment using corneal epithelium forming cell sheets

Country Status (6)

Country Link
US (2) US20060234377A1 (zh)
EP (2) EP1602383B1 (zh)
KR (1) KR101141985B1 (zh)
CN (1) CN1747753B (zh)
ES (1) ES2538696T3 (zh)
WO (1) WO2004069295A1 (zh)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060240552A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato Anterior ocular-associated cell sheet, three-dimensional construct and process for producing the same
US20070148137A1 (en) * 2003-02-03 2007-06-28 Teruo Okano Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
US20080009063A1 (en) * 2006-07-05 2008-01-10 Teruo Okano Substrate for cell culture
US20080118474A1 (en) * 2004-04-25 2008-05-22 Cellseed Inc. Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof
US20080131476A1 (en) * 2005-02-28 2008-06-05 Masato Kanzaki Cultured Cell Sheet, Production Method and Tissue Repair Method Using Thereof
US20080227203A1 (en) * 2007-03-15 2008-09-18 Dai Nippon Printing Co., Ltd. Cell culture support and manufacture thereof
US20080226692A1 (en) * 2005-02-28 2008-09-18 Masato Sato Cultured Cell Sheet, Production Method Thereof, and Application Method Thereof
US20100216242A1 (en) * 2009-02-26 2010-08-26 Kabushiki Kaisha Toyota Chuo Kenkyusho Cell culture support and production method and uses thereof
US20110229962A1 (en) * 2008-10-14 2011-09-22 Manabu Mizutani Temperature-responsive cell culture substrate and method for producing the same
US20170233696A1 (en) * 2014-08-13 2017-08-17 Mitsui Chemicals, Inc. Medical instrument, cell culture method, fluorine-containing cyclic olefin polymer and fluorine-containing cyclic olefin polymer composition for it, and cultured cellls
US20180127700A1 (en) * 2015-05-21 2018-05-10 University Of South Florida Cell Culture Substrate for Rapid Release and Re-Plating
US10434219B2 (en) 2003-02-06 2019-10-08 Cellseed Inc. Method of treatment using corneal epithelium forming cell sheets

Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
JP4834802B2 (ja) 2004-02-18 2011-12-14 聡 山上 ヒト角膜内皮細胞の培養物層積層体及びその作製方法
CN1883438A (zh) * 2005-06-21 2006-12-27 香港理工大学 温度响应性智能面膜及其制备方法
JP4919464B2 (ja) 2006-03-02 2012-04-18 国立大学法人大阪大学 三次元組織の製造方法およびそれに用いる細胞外マトリックスの製造方法。
US9102916B2 (en) 2007-02-27 2015-08-11 Trustees Of Tufts College Tissue-engineered silk organs
EP2682458B1 (en) * 2011-02-28 2019-10-23 CellSeed Inc. Cell sheet having hyaluronic acid production ability and method for preparing the same
CN103892938A (zh) * 2014-04-01 2014-07-02 薛春燕 一种角膜板层片及其制备方法
CN108567995A (zh) * 2017-03-10 2018-09-25 上海睿泰生物科技股份有限公司 一种用于角膜疾病治疗的上皮细胞片的制备方法及其应用
CN110229783A (zh) * 2019-05-22 2019-09-13 中国人民解放军总医院 一种基于温皿制备干细胞膜片的方法

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US20070148137A1 (en) * 2003-02-03 2007-06-28 Teruo Okano Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
US20080118474A1 (en) * 2004-04-25 2008-05-22 Cellseed Inc. Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof
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ES2538696T3 (es) 2003-02-06 2015-06-23 Cellseed Inc. Láminas de células para la formación de la ectocórnea, método para producir las mismas y método para usar las mismas

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US4772283A (en) * 1986-05-16 1988-09-20 White Thomas C Corneal implant
US5284766A (en) * 1989-02-10 1994-02-08 Kao Corporation Bed material for cell culture
US5837278A (en) * 1994-01-06 1998-11-17 Ed Geistlich Sohne Ag Fur Chemische Industrie Resorbable collagen membrane for use in guided tissue regeneration
WO1998031316A1 (en) * 1997-01-17 1998-07-23 Celadon Science, Llc Methods for promoting healing of corneal resurfacing wounds
US6956077B1 (en) * 1999-01-29 2005-10-18 Amersham Biosciences Kk Temperature-responsive polymer compound and process for producing the same
US20030036196A1 (en) * 2000-03-16 2003-02-20 Teruo Okano Cell cultivation-support material, method of cocultivation of cells and cocultivated cell sheet obtained therefrom
US20040028657A1 (en) * 2000-07-21 2004-02-12 Teruo Okano Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same
US20040009566A1 (en) * 2000-07-21 2004-01-15 Teruo Okano Heart muscle-like cell sheet, three-dimensional construct, heart muscle-like tissue and process for producing the same
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US20060240552A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato Anterior ocular-associated cell sheet, three-dimensional construct and process for producing the same
US10434219B2 (en) 2003-02-06 2019-10-08 Cellseed Inc. Method of treatment using corneal epithelium forming cell sheets
US8642338B2 (en) 2003-02-06 2014-02-04 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
US9981064B2 (en) 2003-02-20 2018-05-29 Cellseed Inc. Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
US20080118474A1 (en) * 2004-04-25 2008-05-22 Cellseed Inc. Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof
US9114192B2 (en) 2004-04-25 2015-08-25 Cellseed Inc. Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof
US9889228B2 (en) 2005-02-28 2018-02-13 Cellseed Inc. Cultured cell sheet, production method, and tissue repair method using thereof
US20080131476A1 (en) * 2005-02-28 2008-06-05 Masato Kanzaki Cultured Cell Sheet, Production Method and Tissue Repair Method Using Thereof
US20080226692A1 (en) * 2005-02-28 2008-09-18 Masato Sato Cultured Cell Sheet, Production Method Thereof, and Application Method Thereof
US10533158B2 (en) 2005-02-28 2020-01-14 Tokai University Educational System Cultured cell sheet, production method thereof, and application method thereof
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EP1602383A1 (en) 2005-12-07
US20180126040A1 (en) 2018-05-10
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CN1747753B (zh) 2010-11-10
US10434219B2 (en) 2019-10-08
WO2004069295A1 (ja) 2004-08-19
EP1602383A4 (en) 2009-07-22
EP2450065A1 (en) 2012-05-09
ES2538696T3 (es) 2015-06-23

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