US20060127330A1 - Composition for treating and/or preventing periodontal disease - Google Patents

Composition for treating and/or preventing periodontal disease Download PDF

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Publication number
US20060127330A1
US20060127330A1 US11/340,796 US34079606A US2006127330A1 US 20060127330 A1 US20060127330 A1 US 20060127330A1 US 34079606 A US34079606 A US 34079606A US 2006127330 A1 US2006127330 A1 US 2006127330A1
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Prior art keywords
composition
periodontal disease
sasa
mass
extract
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US11/340,796
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Inventor
Yuuzou Tsuchida
Mitsuo Kawabe
Kunitomo Watanabe
Kotarou Tsuchida
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Hououdou Co Ltd
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Hououdou Co Ltd
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Assigned to HOUOUDOU CO., LTD. reassignment HOUOUDOU CO., LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: KAWABE, MITSUO, TSUCHIDA, KOTAROU, TSUCHIDA, YUUZOU, WATANABE, KUNITOMO
Publication of US20060127330A1 publication Critical patent/US20060127330A1/en
Priority to US11/838,158 priority Critical patent/US8343556B2/en
Priority to US13/710,118 priority patent/US20130101529A1/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/12—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses

Definitions

  • the present invention relates to a composition for treating and/or preventing the periodontal disease, which comprises an extract originated from the plant: Sasa (bamboo grass).
  • Streptococcus mutans is closely involved in the decayed teeth or caries.
  • periodontosis is related to the abnormal proliferation of periodontal disease-related microorganisms within periodontal pockets.
  • known examples of such periodontal disease-related microorganisms include Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides forthythus, Prevotella intermedia, Fusobacterium nucleatum, Capnocytophaga spp., corroding organisms ( Wolinella spp., Campylobacter gracilis , and Eikenella corrodens ), Eubacterium spp., and Treponema spp.
  • Candida albicans has been known as a causal bacterium for the “thrush (candidal stomatitis)” and there have recently been proposed some opinions which point out the close correlation between the abnormal proliferation of Candida spp. within the oral cavity and the periodontosis.
  • the extract from Sasa albo-marginata has an antimicrobial activity.
  • antimicrobial activities as those against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli , which are known as causal bacteria for infectious diseases of wound and those against Helicobacter pylori known as a causal bacteria for the gastric ulcer.
  • the Sasa extract has the antimicrobial activity effective for any kind of bacteria and it has not likewise generally been known that an extract derived from a natural source, which shows an antimicrobial activity against bacteria, also shows an antimicrobial activity against fungi such as Candida spp.
  • the present invention provides a composition for treating and/or preventing the periodontal disease, which comprises an extract originated from the plant: Sasa (bamboo grass) (hereafter referred to as “Sasa extract”).
  • the present invention also provides a composition for treating and/or preventing the periodontal disease, which comprises the Sasa extract and an organic acid.
  • composition for treating and/or preventing the periodontal disease according to the present invention is preferably in the form of a cream.
  • the term “periodontal disease” used is defined so as to include not only the “periodontosis” which has been said to be related to the abnormal proliferation of periodontal disease-related microorganisms, but also the “decayed teeth” in which Streptococcus mutans is closely involved. Accordingly, the composition for the prevention and/or treatment of the periodontal disease according to the present invention likewise includes a composition for treating and/or preventing the “periodontosis” as well as the “decayed teeth”.
  • FIG. 1 is a scanning electron micrograph (SEM) (15,000 ⁇ ) of bacterial plaques grown on a culture medium not containing Sasa extract (TWEBS).
  • FIG. 2 is a scanning electron micrograph (SEM) (15,000 ⁇ ) of bacterial plaques grown on a culture medium in the presence of 0.2% of the Sasa extract (TWEBS).
  • FIG. 3 is a scanning electron micrograph (SEM) (3,000 ⁇ ) of bacterial plaques grown on a culture medium not containing Sasa extract (TWEBS).
  • FIG. 4 is a scanning electron micrograph (SEM) (3,000 ⁇ ) of bacterial plaques grown on a culture medium in the presence of 0.2% of the Sasa extract (TWEBS).
  • the inventors of this invention have variously investigated the antimicrobial activities of the Sasa extract against a variety of bacteria, have found that if the Sasa extract is used in a concentration (as expressed in terms of the solid content) ranging from 1 to 50% by mass, preferably 2 to 25% by mass and more preferably 4 to 15% by mass, it can show conspicuous antimicrobial activities against periodontal disease-related microorganisms (such as Actinobacillus actinomycetem - comitans, Porphyromonas gingivalis, Bacteroides forthythus, Prevotella intermedia, Fusobacterium nucleatum, Capnocytophaga spp., corroding organisms ( Wolinella spp., Campylobacter gracilis, and Eikenella corrodens ), Eubacterium spp.
  • periodontal disease-related microorganisms such as Actinobacillus actinomycetem - comitans, Porphyromona
  • the antimicrobial activity of the Sasa extract can be improved by the incorporation of an organic acid such as malic acid into the same and have thus completed the present invention.
  • the solid content of the Sasa extract is less than 1% by mass, the resulting product would show only a limited effect of treating and/or preventing the periodontal disease, while the use thereof in a concentration of higher than 50% by mass is not preferred because of its extremely high stimulation.
  • Sasa (bamboo grass) used as a raw material for the Sasa extract which is the effective component of the composition for treating and/or preventing the periodontal disease according to the present invention is not restricted to any specific one and all of the plants belonging to Sasa Makino et Shibata.
  • Sasa paniculata Makino et Shibata examples thereof include Sasa paniculata Makino et Shibata, Sasa albo-marginata, Sasa kurilensis Makino et Shibata, OKUYAMA Sasa, EZOMIYAMA Sasa, Sasa palmate Nakai, YAHIKO Sasa, Sasa megalophylla Makino et Uchida, MIYAMA Sasa, SENDAI Sasa, YUKAWA Sasa, ABOI Sasa and ONUKA Sasa.
  • Sasa paniculata Makino et Shibata and Sasa albo-marginata CHUGOKU Sasa and HIDA Sasa.
  • the Sasa extract used in the present invention is preferably one prepared by extracting raw leaves or dried leaves of a Sasa plant, preferably dried leaves thereof with water maintained at a temperature ranging from 100 to 180° C. at ordinary pressure or while applying a pressure.
  • the extraction method is not restricted to any particular one, but usable herein includes, for instance, that disclosed in Japanese Patent No. 3,212,278 (Japanese Un-Examined Patent Publication Hei 11-196818). More specifically, leaves of a Sasa plant are extracted at a temperature ranging from 100 to 180° C. for 5 to 30 minutes using a pressurized hot water extraction device, the resulting extract is separated from a moisture-containing solid content (moisture content: 40 to 70%) in a moisture separator, thereafter the moisture-containing solid content is treated at a temperature ranging from 100 to 200° C. for 5 to 60 minutes in a saturated vapor-heating device, the solid content thus treated is again treated at 100 to 180° C.
  • a moisture-containing solid content moisture content: 40 to 70%
  • an extract obtained by extracting dried leaves of a Sasa plant with, for instance, water heated to 60 to 100° C. for 30 minutes to 12 hours.
  • Examples of commercially available Sasa extracts each comprising 50% by mass of the Sasa extract as expressed in terms of the solid content include “TWEBS” manufactured and sold by Hououdou Co., Ltd. and “AHSS” manufactured and sold by Chloroland Moshiri Co., Ltd.
  • the Sasa extract thus obtained contains sulfur atom-containing components and the content thereof as expressed in terms of the amount of sulfur atom ranges from about 4 to 10 mg and usually about 6 to 9 mg per one gram of the solid content of the Sasa extract. Principal constituents of the sulfur atom-containing components are considered to be sulfur atom-containing amino acids.
  • composition for treating and/or preventing periodontal disease comprises such sulfur atom-containing components derived from the Sasa extract in an amount preferably ranging from 4 to 500 mg, more preferably 8 to 250 mg and most preferably 16 to 150 mg per 100 g of the composition as expressed in terms of the amount of sulfur.
  • the Sasa extract comprises tannin and the content thereof ranges from about 5 to 15% by mass on the basis of the solid content of the extract.
  • composition for treating and/or preventing periodontal disease according to the present invention comprises tannin originated from the Sasa plant in an amount preferably ranging from 0.05 to 7.5% by mass and more preferably 0.1 to 6% by mass as expressed in terms of the solid content thereof.
  • composition for treating and/or preventing periodontal disease according to the present invention may be one simply comprising the Sasa extract as an effective component, but the antimicrobial activity thereof can further be improved by the use of an appropriate amount of an organic acid in combination with the Sasa extract.
  • organic acids are malic acid, citric acid, lactic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, acetic acid, benzoic acid, phenylacetic acid, salicylic acid and phenols.
  • malic acid particularly preferably used herein is malic acid.
  • the amount of the organic acid to be used preferably ranges from 0.01 to 5% by mass, more preferably 0.02 to 3% by mass and most preferably 0.05 to 1.5% by mass on the basis of the total mass of the composition for treating and/or preventing periodontal disease.
  • composition for the treatment and/or prevention of the periodontal disease according to the present invention may comprise only the Sasa extract or the combination of the Sasa extract and an organic acid, or the resulting composition may further admixed with other components and/or a carrier.
  • the dosage forms of the composition for the treatment and/or prevention of the periodontal disease may be liquid, solid or gaseous ones.
  • the composition for the treatment and/or prevention of the periodontal disease may be administered through either the oral route or any parenteral route.
  • Examples of the dosage forms of the composition are a tablet, a pill, a powdery preparation, a liquid preparation, a chewing gum, or various forms of foods such as a candy or starch jelly, a chocolate, bread, a cookie, buckwheat vermicelli and wheat vermicelli for the oral administration; and a tooth paste, a mouth wash, and dosage forms for the local administration (such as a cream and an ointment), for the parenteral administration.
  • Examples of such dosage forms for the local administration are those obtained by impregnating a carrier such as gauze made of natural or synthetic fibers with the composition for the prevention and/or treatment of the periodontal disease according to the present invention.
  • those for the local administration such as a cream and an ointment are preferably used since they are suitable for the direct application to an affected part suffering from the periodontal disease or a decayed tooth.
  • a base component such as an oily component, a humectant and/or an antiseptic, which are commonly used in pharmaceutical compositions, cosmetic products, compositions applied to the skin and compositions intra-orally administered (such as a tooth paste and a mouth wash).
  • Water used in the composition for the prevention and/or treatment of the periodontal disease are not restricted to particular ones and examples thereof include tap water, natural water and purified water, but preferably used herein is highly purified water such as ion-exchange water.
  • oils derived from animals such as squalane, tallow, lard, horse fat, lanolin and beeswax
  • oils derived from vegetables such as olive oil, grape seed oil, palm oil, jojoba oil and germ oil (such as rice germ oil)
  • synthetic or semi-synthetic oils such as liquid paraffin, higher fatty acid esters (such as octyl palmitate, isopropyl palmitate and octyl dodecyl myristate) and silicone oil.
  • the oily components are used in appropriate combinations while taking into consideration the performance requirement, for instance, an ability of protecting the skin, an effect of imparting emollient (or an effect of preventing drying of the skin and imparting softness and resilience to the skin through the coverage of the skin surface with a thin film) and an ability of imparting refreshed feeling to the skin.
  • the oily component comprises squalane, olive oil and octyl dodecyl myristate.
  • composition for the treatment and/or prevention of the periodontal disease comprises a solid oil component such as stearic acid, stearyl alcohol, behenic acid, cetanol and vaseline to control the hardness and flowability of the resulting composition and the composition preferably comprises stearic acid and cetanol in combination.
  • a solid oil component such as stearic acid, stearyl alcohol, behenic acid, cetanol and vaseline to control the hardness and flowability of the resulting composition and the composition preferably comprises stearic acid and cetanol in combination.
  • a creaming agent is used to convert the mixture of the Sasa extract, water and an oily component into a cream.
  • a creaming agent is not restricted to any particular one, but glycerin monostearate and a self-emulsifiable glycerin monostearate (a product obtained by incorporating an emulsifying agent into glycerin monostearate) are in general used in combination.
  • composition for the treatment and/or prevention of the periodontal disease according to the present invention may, if necessary, comprise other additives such as a stabilizer, a humectant (a wetting agent), a wound-healing agent, an antiseptic, a surfactant, a binder, a foaming agent, a sweetening agent, a refrigerant and/or an abrasive.
  • a stabilizer such as a humectant (a wetting agent), a wound-healing agent, an antiseptic, a surfactant, a binder, a foaming agent, a sweetening agent, a refrigerant and/or an abrasive.
  • a humectant a wetting agent
  • a wound-healing agent such as an antiseptic, a surfactant, a binder, a foaming agent, a sweetening agent, a refrigerant and/or an abrasive.
  • stabilizers are a combination of a carboxy vinyl polymer with potassium hydroxide; polyethylene glycol distearate; and magnesium phosphate.
  • polyethylene glycol sesqui-stearate a 1:1 mixture of polyethylene glycol distearate and polyethylene glycol monostearate
  • the molecular weight of the polyethylene glycol ranging from 1000 to 20,000 is preferably used herein since it has high stability, is not separated into water and oil and the hardness required when the composition is applied to the skin in the form of a cream composition can effectively be controlled.
  • humectants usable herein are sodium salt of hyaluronic acid, collagen, an aloe extract (in particular, the aloe extract (2) derived from Aloe arborescens is preferred), urea, 1,3-butylene glycol, glycerin, trehalose, sorbitol, amino acids and sodium salt of pyrrolidone carboxylic acid.
  • wound-healing agents examples include allantoin, di-potassium glycyrrhizinate, a glycyrrhiza extract and a mugwort extract.
  • antiseptic is used subsidiarily since the Sasa extract has an antibiotic effect by nature.
  • antiseptics are sodium benzoate, lower alkyl esters of p-hydroxy benzoic acid (for instance, so-called paraben such as methyl, ethyl, propyl or butyl ester), sodium propionate, mixed fatty acid esters (a mixture of capric acid glyceryl, lauric acid glyceryl-2 and lauric acid polyglyceryl-10), phenoxy ethanol, light-sensitive substance No. 201 (yellow dye), and 1,2-pentanediol, with paraben, mixed fatty acid esters and 1,2-pentanediol being preferably used herein.
  • surfactants are sodium N-acyl-L-glutamate and polyoxyethylene sorbitan monostearate.
  • Examples of the binders usable herein include sodium carboxymethyl cellulose; examples of the foaming agents are sodium lauryl sulfate, sodium lauroyl glutamate, and sodium lauroyl sarcosinate; examples of the sweetening agents are xylitol, sorbitol and saccharin sodium; examples of the refrigerants include mint essence; and examples of the abrasives are calcium phosphate, calcium hydrogen phosphate and silica.
  • composition may, if required, comprise aroma components such as orange oil, lemon oil, bitter orange peel oil and perfumes.
  • Table 1 shows preferred amounts (% by mass) of the foregoing ingredients required for preparing the composition for the treatment and/or prevention of the periodontal disease in the form of a cream composition.
  • the amount of each component other than water is based on the total mass of the foregoing components other than water.
  • the foregoing components are introduced into a heating-mixing kettle equipped with a stirring blade and preferably an emulsifying apparatus and they are then admixed together at a temperature ranging from 70 to 90° C. for 1 to 2 hours to thus form a composition for treating and/or preventing the periodontal disease according to the present invention.
  • composition for the treatment and/or prevention of the periodontal disease according to the present invention may be used in a variety of dosage forms such as an ointment, a liquid preparation, a jelly preparation, a gel-like preparation, an aerosol and other shapes, in addition to a cream composition, with a cream-like composition being preferably used herein because it can easily be used and show a considerably excellent effect.
  • the composition of the present invention may likewise be formed into a semi-solid or liquid product.
  • the composition for the treatment and/or prevention of the periodontal disease according to the present invention to the affected part such as the affected part of periodontal disease, the tooth root of a decayed tooth or the periodontium, in an appropriate amount on the order of, for instance, 0.1 to 1 g, at a frequency ranging from 1 to 5 times, usually 1 to 3 times a day, when it is used in the form of a cream composition.
  • the amount and frequency of application of the composition may appropriately be changed while taking into consideration, for instance, the symptoms of the disease.
  • composition for the treatment and/or prevention of the periodontal disease according to the present invention is desirably taken in an appropriate amount on the order of, for instance, 0.01 to 0.1 g/l kg of body weight at a frequency ranging from 1 to 5 times, usually 1 to 3 times a day, when it is administered through the parenteral route.
  • the amount and frequency of application of the composition may appropriately be changed while taking into consideration, for instance, the symptoms of the disease.
  • the Sasa extract used in the composition for the treatment and/or prevention of the periodontal disease according to the present invention as the effective component is an extract originated from a plant belonging to the genus Sasa Makino et Shibata and a 1.25% by mass aqueous solution thereof does not show any toxicity to the 293 cells derived from human fetal kidney.
  • composition for the treatment and/or prevention of the periodontal disease according to the present invention comprises the Sasa extract in an amount ranging from 1 to 50% by mass as expressed in terms of the solid content thereof and the composition thus shows significant antimicrobial activities against microorganisms such as periodontal disease-related microorganisms and bacteria belonging to Candida.
  • compositions for the treatment and/or prevention of the periodontal disease according to the present invention are preferred embodiments of the composition for the treatment and/or prevention of the periodontal disease according to the present invention:
  • a composition for the treatment and/or prevention of the periodontal disease comprises the Sasa extract (1 to 50% by mass as expressed in terms of the solid content), water, an oily component and a creaming agent.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 in which the oily component is at least one member selected from the group consisting of animal oils, vegetable oils, synthetic oils and semi-synthetic oils.
  • the oily component is at least one member selected from the group consisting of squalane, tallow, lard, horse fat, lanolin, beeswax, olive oil, grape seed oil, palm oil, jojoba oil, germ oil, liquid paraffin, octyl palmitate, isopropyl palmitate, octyl dodecyl myristate, silicone
  • composition for the treatment and/or prevention of the periodontal disease as set forth in any one of the foregoing items 1 to 3 in which the creaming agent is a combination of glycerin monostearate with self-emulsifiable glycerin monostearate.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in any one of the foregoing items 1 to 4 in which it further comprises at least one component selected from the group consisting of an organic acid, a stabilizer, a humectant, a wound-healing agent, an antiseptic and a surfactant.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 5 in which the humectant is at least one member selected from the group consisting of sodium salt of hyaluronic acid, collagen, aloe extracts, urea, 1,3-butylene glycol, glycerin, trehalose, sorbitol, amino acids and sodium salt of pyrrolidone carboxylic acid.
  • the humectant is at least one member selected from the group consisting of sodium salt of hyaluronic acid, collagen, aloe extracts, urea, 1,3-butylene glycol, glycerin, trehalose, sorbitol, amino acids and sodium salt of pyrrolidone carboxylic acid.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 5 in which the antiseptic is at least one member selected from the group consisting of sodium benzoate, lower alkyl esters of p-hydroxy benzoic acid, sodium propionate, mixed fatty acid esters, phenoxy ethanol, 1,2-pentanediol and yellow dyes.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 5 which further comprises at least one member selected from the group consisting of orange oil, lemon oil, bitter orange peel oil and perfumes.
  • a composition for the treatment and/or prevention of the periodontal disease which comprises the Sasa extract, water, an oily component, a creaming agent, a stabilizer, a humectant, a wound-healing-promoting agent, an antiseptic and a surfactant
  • the oily component is at least one member selected from the group consisting of squalane, tallow, lard, horse fat, lanolin, beeswax, olive oil, grape seed oil, palm oil, jojoba oil, germ oil, liquid paraffin, octyl palmitate, isopropyl palmitate, octyl dodecyl myristate, silicone oil, stearic acid, stearyl alcohol, behenic acid, cetanol and vaseline;
  • the creaming agent is a combination of glycerin monostearate with self-emulsifiable glycerin monostearate;
  • the stabilizer is at least one member selected from the group consisting
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 11 which further comprises at least one member selected from the group consisting of orange oil, lemon oil, bitter orange peel oil and perfumes.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 which comprises the Sasa extract, water, squalane, olive oil, glycerin monostearate, self-emulsifiable glycerin monostearate, a carboxy vinyl polymer, potassium hydroxide, urea, 1,3-butylene glycol, allantoin, a lower alkyl ester of p-hydroxy benzoic acid, stearic acid, sodium N-acyl-L-glutamate and lemon oil.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 which comprises the Sasa extract, water, squalane, olive oil, octyl dodecyl myristate, cetanol, glycerin monostearate, self-emulsifiable glycerin monostearate, a carboxy vinyl polymer, potassium hydroxide, urea, 1,3-butylene glycol, allantoin, a mixed fatty acid ester, stearic acid, sodium N-acyl-L-glutamate and orange oil.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in any one of the foregoing items 1 to 14 which comprises polyethylene glycol sesqui-stearate.
  • the organic acid is at least one member selected from the group consisting of malic acid, citric acid, lactic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, acetic acid, benzoic acid, phenylacetic acid, salicylic acid and phenols.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 which comprises calcium hydrogen phosphate, water, sorbitol, glycerin, the Sasa extract, silica, cellulose gum, sodium lauryl sulfate, xylitol, mint essence, magnesium phosphate, sodium lauroyl sarcosine and sodium saccharine.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 which comprises calcium hydrogen phosphate, water, sorbitol, glycerin, the Sasa extract, silica, cellulose gum, sodium lauroyl glutamate, xylitol, mint essence, magnesium phosphate and sodium lauroyl sarcosine.
  • composition for the treatment and/or prevention of the periodontal disease as set forth in the foregoing item 1 which comprises water, sorbitol, glycerin, the Sasa extract, cellulose gum, sodium lauroyl glutamate, xylitol, mint essence, magnesium phosphate and sodium lauroyl sarcosine.
  • the resulting Sasa extract was inspected for the sulfur content and it was found to be 3850 ⁇ m/ml (7.7 mg per one gram of the solid content).
  • the added amounts of a Sasa extract having a solid content of 8% by mass were 12.5, 25, 37.5 and 75% by mass in Examples 1 to 4, respectively (therefore, the contents of the extract as expressed in terms of the solid contents thereof were 1, 2, 3 and 6% by mass; and sulfur contents of these samples were 7.7 mg, 15.4 mg, 23.1 mg and 46.2 mg per 100 g of the composition for treating and/or preventing the periodontal disease, respectively).
  • composition for treating and/or preventing the periodontal disease was found to have a sulfur content of 46.2 mg per 100 g of the composition.
  • the pH value of the resulting cream was found to be 6.00.
  • the pH value of the resulting cream was found to be 4.68.
  • Example 7 The same procedures used in Example 7 except that the amounts of the Sasa extract (solid content: 50% by mass) were set at 16%, 20% and 30% by mass and that the amount of purified water was adjusted in proportion thereto to thus give cream compositions for treating and/or preventing the periodontal disease according to the present invention, each having a content of the Sasa extract (solid content) of 8%, 10% or 15% by mass, respectively.
  • TWEBS a solution containing 50% by mass (as expressed in terms of the solid content) of the Sasa extract and 1% by mass of malic acid and having a pH value of about 5.0) available from HOUOUDOU CO., LTD. Sterilized distilled water was used as a diluent.
  • the test solution was in order diluted twofold to concentrations of 4, 2, 1 and 0.5%, while the concentration thereof was variously changed to 8, 7, 6, 5, 4, 3, 2 and 1% for the fungi belonging to Candida spp. to thus determine each corresponding minimum inhibitory concentration (MIC).
  • the minimum inhibitory concentration (MIC) was determined according to the agar plate-dilution technique using a GAM agar culture medium as the sensitivity-determining medium. The judgment was carried out after the cultivation under aerobic conditions over 24 hours for the facultative bacteria and after the cultivation under anaerobic conditions over 48 hours for the anaerobic bacteria. Each bacterium was inoculated at a bacterial density of about 10 6 cells per spot. A 2N NaOH aqueous solution or a 10% hydrogen chloride aqueous solution was, if desired, used to control the pH value of the culture medium.
  • a fungus belonging to Candida spp. was cultivated on a Mycocell-agar culture medium (BD) over 48 hours to thus obtain a colony of each strain to be tested (test strain), the colony was dispersed in an MH broth (available from Difco) to obtain a bacterial cell dispersion having a turbidity of McFarland#1, and then one platinum loop (10 ⁇ l) each of the resulting bacterial cell dispersion was inoculated into a series of sample solution-containing plate culture mediums according to the streaking-smearing technique. In this respect, one platinum loop of the resulting bacterial cell dispersion included about 10 6 bacterial cells. After cultivating each plate culture medium at 35° C. for 24 hours, the culture mediums were visually observed for the confirmation of whether the bacterial cells underwent proliferation or not. The case wherein the bacterial cells underwent proliferation was judged to be (+).
  • Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were used as the strains for the management of the MIC precision.
  • Streptococcus mutans GTC 218 As an example of the decayed tooth-related bacteria.
  • control bacteria 5 strains of gram-positive cocci including Streptococcus pyogenes ATCC 19615 and Streptococcus pneumoniae ATCC 6305. These bacteria are pathogenic bacteria causing diseases of upper respiratory tracts such as the oral cavity and the pharyngeal cavity and it has been recognized that they do not belong to such decayed tooth-related bacteria. Pure-cultured bacteria aerobically cultivated on a blood-agar culture medium were used in the experiments.
  • Periodontal disease-related microorganisms Porphyromonas gingivalis ATCC 33277, Prevotella intermedia ATCC 25611, Bacteroides forthythus, Fusobacterium nucleatum ATCC 25586, Actinobacillus actinomycetemcomitans , and Capnocytophaga ohracea GAI-5586. These bacteria were subjected to the pure-cultivation on a Brucella HK blood-agar culture medium and the resulting pure-cultivated bacteria were used. In the cultivation, there were used anaerobic glove-boxes.
  • Candida albicans There were used 13 strains of Candida albicans and Candida glabrata in all. These strains were isolated from various kinds of clinical materials and stored in the anaerobic bacteria-experimental facilities affiliated with the medical department of Gifu University.
  • the Sasa extract of the present invention inhibited the growth of S. mutans at a concentration of 2% under acidic conditions (at a pH value ranging from 6.0 to 5.0).
  • the Sasa extract likewise inhibited the growth of the 4 kinds of periodontal disease-related bacteria examined above at a concentration of 2% under the same growth conditions.
  • the Sasa extract inhibited the growth of the fungi belonging to Candida spp. at a concentration of 5%.
  • the term “dental plaque” herein used means the bio-film formed on a tooth.
  • This bio-film is constituted by a colony or mass of a plurality of bacteria living in and buried in the polymers originated from the bacteria and/or those originated from the saliva (Eps: Extracellular polysaccharide substances or glycocalyx).
  • the bio-film formed on the surface of a tooth is characterized in that it is quite hardly peeled off unlike those formed on the surface of the oral mucosa and that of the mucosa of tongue and further it would hardly be affected by a variety of antibacterial substances. Accordingly, it has been recognized that the dental plaque is quite important as a cause of the periodontal disease. It is important to destroy the same for the treatment of the periodontal disease and therefore, various studies have been conducted.
  • Pg bacterium is one of the most important anaerobic bacteria relating to the periodontal disease and it has been known that the bacterium can produce a powerful protease and that it plays an important role in the destruction of the gingiva.
  • the Pg bacteria extracellularly produce glycocalyx.
  • the protease produced by the Pg bacteria is extracellularly released by vesicles.
  • TWEBS has an extremely high antimicrobial activity against the Pg bacteria and that it can inhibit the growth of the bacteria at a concentration of 0.5%.
  • TWEBS was investigated for the effect thereof on the glycocalyx-producing ability of the Pg bacteria.
  • the minimum inhibitory concentration (MIC) of TWEBS for this bacterial strain is not more than 0.5%.
  • Bacteria were cultivated according to the anaerobic cultivation technique using ANEROPACK (available from Sugiyamagen Co., Ltd).
  • the Pg bacteria were grown on the modified GAM agar culture medium containing the sub-MIC concentration of TWEBS and the resulting bacterial plaques were observed under an electron microscope (SEM, TEM).
  • TWEBS inhibits the proliferation of Pg bacteria at such a lower concentration on the order of not more than 0.5% (MIC: 0.5%)
  • results obtained in Test Example 2 clearly indicate that TWEBS prevents the production or release of an extracellular substance originated from the Pg bacteria, which may be considered as glycocalyx, at the so-called sub-MIC concentration (0.2%) lower than the MIC.
  • composition of the present invention is useful as a composition for treating and/or preventing periodontal disease such as “periodontosis” which is assumed to be related to the abnormal proliferation of periodontal disease-related microorganisms within periodontal pockets and “decayed tooth” in which Streptococcus mutans is closely involved.
  • periodontal disease such as “periodontosis” which is assumed to be related to the abnormal proliferation of periodontal disease-related microorganisms within periodontal pockets and “decayed tooth” in which Streptococcus mutans is closely involved.
US11/340,796 2003-07-29 2006-01-27 Composition for treating and/or preventing periodontal disease Abandoned US20060127330A1 (en)

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US20170100328A1 (en) * 2011-02-04 2017-04-13 Joseph E. Kovarik Bioadhesive Strip and Method of Using Same
US11826388B2 (en) 2013-12-20 2023-11-28 Seed Health, Inc. Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation
US11833177B2 (en) 2013-12-20 2023-12-05 Seed Health, Inc. Probiotic to enhance an individual's skin microbiome
US11839632B2 (en) 2013-12-20 2023-12-12 Seed Health, Inc. Topical application of CRISPR-modified bacteria to treat acne vulgaris
US11844720B2 (en) 2011-02-04 2023-12-19 Seed Health, Inc. Method and system to reduce the likelihood of dental caries and halitosis
US11951139B2 (en) 2015-11-30 2024-04-09 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US11951140B2 (en) 2011-02-04 2024-04-09 Seed Health, Inc. Modulation of an individual's gut microbiome to address osteoporosis and bone disease
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WO2005009454A1 (ja) * 2003-07-29 2005-02-03 Hououdou Co., Ltd. 歯周病治療用及び／又は予防用組成物
EP2279662A3 (en) * 2006-03-10 2011-04-13 Yuuzou Tsuchida 5,7,4'-trihydroxy-3',5'-dimethoxyflavone as an antibacterial agent
JP2009274957A (ja) * 2006-08-28 2009-11-26 Univ Nihon ベニカミキリフラスを有効成分とする歯周病原菌殺菌薬
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JP5511183B2 (ja) * 2007-12-28 2014-06-04 アース製薬株式会社 有機酸による歯のエナメル質の溶解が阻害される口腔用組成物
KR101950662B1 (ko) * 2011-09-28 2019-02-20 라이온 가부시키가이샤 구강용 조성물
CN105142607B (zh) * 2013-03-27 2018-12-04 狮王株式会社 口腔用组合物
WO2015099754A1 (en) * 2013-12-27 2015-07-02 Colgate-Palmolive Company Prebiotic oral care compositions containing carboxylic acids
JP6756553B2 (ja) * 2016-09-09 2020-09-16 株式会社 アルマード ジェル状歯磨き剤
JP6914654B2 (ja) * 2016-12-28 2021-08-04 ライオン株式会社 口腔用組成物
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US7569235B2 (en) 2002-03-27 2009-08-04 Hououdou Co., Ltd. Compositions for treating and/or preventing pollinosis
US20170100328A1 (en) * 2011-02-04 2017-04-13 Joseph E. Kovarik Bioadhesive Strip and Method of Using Same
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US11951140B2 (en) 2011-02-04 2024-04-09 Seed Health, Inc. Modulation of an individual's gut microbiome to address osteoporosis and bone disease
US11826388B2 (en) 2013-12-20 2023-11-28 Seed Health, Inc. Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation
US11833177B2 (en) 2013-12-20 2023-12-05 Seed Health, Inc. Probiotic to enhance an individual's skin microbiome
US11839632B2 (en) 2013-12-20 2023-12-12 Seed Health, Inc. Topical application of CRISPR-modified bacteria to treat acne vulgaris
US11969445B2 (en) 2013-12-20 2024-04-30 Seed Health, Inc. Probiotic composition and method for controlling excess weight, obesity, NAFLD and NASH
US11951139B2 (en) 2015-11-30 2024-04-09 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US11980643B2 (en) 2023-08-15 2024-05-14 Seed Health, Inc. Method and system to modify an individual's gut-brain axis to provide neurocognitive protection

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