US20050142648A1 - Modified yeast consuming L-arabinose - Google Patents

Modified yeast consuming L-arabinose Download PDF

Info

Publication number
US20050142648A1
US20050142648A1 US10/983,951 US98395104A US2005142648A1 US 20050142648 A1 US20050142648 A1 US 20050142648A1 US 98395104 A US98395104 A US 98395104A US 2005142648 A1 US2005142648 A1 US 2005142648A1
Authority
US
United States
Prior art keywords
gene
arabinose
strain
yeast
ethanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/983,951
Other languages
English (en)
Inventor
Eckhard Boles
Jessica Becker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Forskarpatent I SYD AB
Original Assignee
Forskarpatent I SYD AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26655701&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=US20050142648(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from SE0201428A external-priority patent/SE0201428D0/xx
Application filed by Forskarpatent I SYD AB filed Critical Forskarpatent I SYD AB
Priority to US10/983,951 priority Critical patent/US20050142648A1/en
Assigned to FORSKARPATENT I SYD AB reassignment FORSKARPATENT I SYD AB ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BECKER, JESSICA, BOLES, ECKHARD
Publication of US20050142648A1 publication Critical patent/US20050142648A1/en
Priority to US11/498,002 priority patent/US20060270008A1/en
Priority to US12/325,630 priority patent/US8691554B2/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention relates to a modified yeast strain, preferably a Saccharomyces cerevisiae, consuming L-arabinose while producing ethanol, as well as a method for producing ethanol.
  • araA L-arabinose isomerase
  • araB L-ribulokinase
  • araD L-ribulose-5-P 4-epimerase
  • transformants expressing the B. subtilis araA gene together with the E. coli genes araB and araD as well as the yeast GAL2 gene were incubated in liquid media (synthetic complete or synthetic complete/0.1% yeast extract/0.2% peptone) with L-arabinose as the sole carbon source for several weeks. After 4-5 days of incubation the transformants started to grow slowly in these media, in contrast to a strain containing only four empty vectors. Whenever the cells reached an OD 600 of 3-4, they were inoculated in fresh medium at an OD 600 of 0.3, and grown further. Growth became faster after 10 days. These observations indicate the occurrence of spontaneous suppressor mutations enabling the cells to use L-arabinose more efficiently. Otherwise, the cells might become somehow adapted to the use of L-arabinose.
  • mutant transformants When the mutant transformants were selected for loss of their plasmids they were no longer able to grow on arabinose.
  • the plasmids were re-isolated and amplified in E. coli.
  • the re-isolated plasmids were transformed into a CEN.PK2-1C wild-type strain.
  • the lag-phase on arabinose medium was significantly prolonged indicating that additional genomic mutations had occurred in the mutant transformants enabling them to grow efficiently on arabinose.
  • Different combinations of original and re-isolated plasmids were transformed into the mutant JBY25 strain.
  • the mutant strain was transformed with different combinations of re-isolated and empty plasmids (without any gene for L-arabinose metabolism).
  • Transformants lacking the L-arabinose isomerase, the L-ribulokinase or the L-ribulose 5-P 4-epimerase but transformed with the other three re-isolated plasmids did not show any growth on L-arabinose indicating that these genes are absolutely necessary for the utilization of L-arabinose.
  • Transformants lacking the overexpressed galactose permease are able to grow on L-arabinose medium, but with slightly decreased growth rates as compared to the mutant strain containing all four re-isolated plasmids, indicating that over-expression of a transporter is not necessary for growth on L-arabinose but can improve it.
  • the mutant strain and also the wild-type strain each transformed with the four plasmids for L-arabinose metabolism were crossed with a haploid wild-type strain. Afterwards, growth on L-arabinose was investigated. The diploid mutant strain exhibited faster growth on L-arabinose than the diploid control strain. But the diploid mutant strain did not grow as well as the haploid mutant strain transformed with the four plasmids. The diploid mutant strain was sporulated and tetrade analysis was performed. The results indicate that there is more than one mutation in the genome of the strain with at least one being dominant and another one being recessive.
  • the growth medium will contain about 20 g of L-arabinose/L. However, growth and production of ethanol will occur between 2 and 200 g/L. There is no need for further sugars, and thus L-arabinose can be used alone. It is possible that co-consumption of xylose and arabinose could work, but this has not been determined so far.

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
US10/983,951 2002-05-08 2004-11-08 Modified yeast consuming L-arabinose Abandoned US20050142648A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/983,951 US20050142648A1 (en) 2002-05-08 2004-11-08 Modified yeast consuming L-arabinose
US11/498,002 US20060270008A1 (en) 2002-05-08 2006-08-02 Modified yeast consuming L-arabinose
US12/325,630 US8691554B2 (en) 2002-05-08 2008-12-01 Modified yeast consuming L-arabinose

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
SE0201428A SE0201428D0 (sv) 2002-05-08 2002-05-08 A modified yeast consuming l-arabinose
SE0201428-0 2002-05-08
SE0202090A SE0202090D0 (sv) 2002-05-08 2002-07-04 A modifierd yeast consuming L-arabinose
SE0202090-7 2002-07-04
PCT/SE2003/000749 WO2003095627A1 (en) 2002-05-08 2003-05-07 A modified yeast consuming l-arabinose
US10/983,951 US20050142648A1 (en) 2002-05-08 2004-11-08 Modified yeast consuming L-arabinose

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2003/000749 Continuation WO2003095627A1 (en) 2002-05-08 2003-05-07 A modified yeast consuming l-arabinose

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/498,002 Continuation US20060270008A1 (en) 2002-05-08 2006-08-02 Modified yeast consuming L-arabinose

Publications (1)

Publication Number Publication Date
US20050142648A1 true US20050142648A1 (en) 2005-06-30

Family

ID=26655701

Family Applications (3)

Application Number Title Priority Date Filing Date
US10/983,951 Abandoned US20050142648A1 (en) 2002-05-08 2004-11-08 Modified yeast consuming L-arabinose
US11/498,002 Abandoned US20060270008A1 (en) 2002-05-08 2006-08-02 Modified yeast consuming L-arabinose
US12/325,630 Active 2025-08-26 US8691554B2 (en) 2002-05-08 2008-12-01 Modified yeast consuming L-arabinose

Family Applications After (2)

Application Number Title Priority Date Filing Date
US11/498,002 Abandoned US20060270008A1 (en) 2002-05-08 2006-08-02 Modified yeast consuming L-arabinose
US12/325,630 Active 2025-08-26 US8691554B2 (en) 2002-05-08 2008-12-01 Modified yeast consuming L-arabinose

Country Status (13)

Country Link
US (3) US20050142648A1 (de)
EP (1) EP1499708B2 (de)
AT (1) ATE315079T1 (de)
AU (1) AU2003228189A1 (de)
BR (2) BRPI0309836B1 (de)
CA (1) CA2483997C (de)
DE (1) DE60303127T3 (de)
DK (1) DK1499708T4 (de)
ES (1) ES2256742T5 (de)
PT (1) PT1499708E (de)
SE (1) SE0202090D0 (de)
WO (1) WO2003095627A1 (de)
ZA (1) ZA200408440B (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007143247A2 (en) * 2006-06-02 2007-12-13 Midwest Research Institute Cloning and characterization of l-arabinose transporters from non-conventional yeast
WO2007143245A2 (en) * 2006-06-01 2007-12-13 Midwest Research Institute An l-arabinose fermenting yeast
US20100151548A1 (en) * 2007-04-05 2010-06-17 Eckhard Boles vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production
WO2017176875A1 (en) 2016-04-08 2017-10-12 E I Du Pont De Nemours And Company Arabinose isomerases for yeast
US10947515B2 (en) 2015-03-16 2021-03-16 Dsm Ip Assets B.V. UDP-glycosyltransferases

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1863901A1 (de) 2005-03-11 2007-12-12 Forskarpatent i Syd AB Arabinose und xylose vergärende saccharomyces cerevisiae-stämme
JP2010501013A (ja) 2006-08-18 2010-01-14 アイオジェン エナジー コーポレイション 水性糖ストリームから有機塩又は有機酸を得る方法
AU2007302867B2 (en) 2006-10-02 2013-11-07 Dsm Ip Assets B.V. Metabolic engineering of arabinose-fermenting yeast cells
SE530337C2 (sv) * 2007-06-12 2008-05-06 Loxystem Ab Förfarande, system och lås för att bestämma den relativa positionen för ett containerhörnlås och/eller en grupp av containerhörnlås i förhållande till andra containerhörnlås
PT103780A (pt) * 2007-07-06 2009-01-06 Univ Nova De Lisboa Sequência de dna que codifica um transportador específico para l- arabinose, molécula de cdna, plasmideo compreendendo a referida sequência de dna, célula hospedeira transformada com esse plamídeo e sua aplicação.
EP2171038A2 (de) * 2007-07-19 2010-04-07 Royal Nedalco B.V. Neue arabinose vergärende eukaryontische zellen
CN104017741B (zh) * 2008-03-07 2018-03-09 帝斯曼知识产权资产管理有限公司 发酵戊糖的细胞
WO2010059539A2 (en) 2008-11-20 2010-05-27 New England Biolabs, Inc. Genetically engineered yeast for the production of biofuels
EP3415613A1 (de) 2008-12-24 2018-12-19 DSM IP Assets B.V. Xylose-isomerase-gene und deren verwendung bei der fermentation von pentosezucker
US20110143408A1 (en) * 2009-06-18 2011-06-16 E. I. Du Pont De Nemours And Company Zymomonas with improved arabinose utilization
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
CN102869766B (zh) 2010-04-21 2015-11-25 帝斯曼知识产权资产管理有限公司 适于发酵混合的糖组合物的细胞
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
MY160995A (en) 2010-10-13 2017-03-28 Dsm Ip Assets Bv Polypeptides with permease activity
CA2834053C (en) 2010-11-18 2019-03-26 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from glycerol
FR2968313B1 (fr) 2010-12-03 2014-10-10 Lesaffre & Cie Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
BR112013026193A2 (pt) 2011-04-11 2020-11-10 Cargill, Incorporated composições e métodos para a produção aumentada de etanol a partir de biomassa
US20140141473A1 (en) * 2011-04-22 2014-05-22 Dsm Ip Assets B.V. Yeast cell capable of converting sugars including arabinose and xlose
EP2546336A1 (de) 2011-07-11 2013-01-16 DSM IP Assets B.V. Hefestränge, die Uronsäure verbrauchen und Fermentierungsprodukte wie Ethanol herstellen
EP2554668A1 (de) 2011-08-04 2013-02-06 DSM IP Assets B.V. Zelle zur Fermentierung von Pentosezucker
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
KR20140099251A (ko) 2011-11-30 2014-08-11 디에스엠 아이피 어셋츠 비.브이. 아세트산 및 글리세롤로부터 에탄올을 생성하도록 합성된 이스트 스트레인
CN104203005A (zh) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
WO2014033018A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
DK2909305T3 (en) 2012-10-16 2019-04-15 Dsm Ip Assets Bv CELLS WITH IMPROVED PENTOSE CONVERSION
KR20150082241A (ko) 2012-11-07 2015-07-15 디에스엠 아이피 어셋츠 비.브이. pH 제어된 효모 증식
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
EP3502264A3 (de) 2013-05-31 2019-11-06 DSM IP Assets B.V. Mikroorganismen zur herstellung von diterpen
EP3885443A1 (de) 2013-07-15 2021-09-29 DSM IP Assets B.V. Production de diterpène
US20160185813A1 (en) 2013-07-31 2016-06-30 Dsm Ip Assets B.V. Recovery of steviol glycosides
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
CN107074918B (zh) 2014-10-22 2021-07-30 布塔尔格有限公司 Gal2转运体的变异体和其用途
RS56018B1 (sr) * 2014-11-26 2017-09-29 Clariant Int Ltd Oligonukleotidna sekvenca za upotrebu u metaboličkom inženjeringu
EP3250698B1 (de) 2015-01-28 2019-11-27 DSM IP Assets B.V. Verfahren zur enzymatischen hydrolyse von lignocellulosestoffen und zuckerfermentation
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
US10435719B2 (en) 2015-01-28 2019-10-08 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3259359B1 (de) * 2015-02-16 2020-11-18 Deinove L-arabinose-assimilationspfad und verwendungen davon
WO2016151046A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
CN113683712B (zh) 2015-04-03 2022-10-21 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
EP3286303A1 (de) 2015-04-21 2018-02-28 DSM IP Assets B.V. Mikrobielle herstellung von terpenoiden
FR3035405B1 (fr) 2015-04-27 2019-04-19 Lesaffre Et Compagnie Souche de levure presentant une capacite amelioree a fermenter le xylose en presence d'acide acetique
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP4043579A1 (de) 2015-07-10 2022-08-17 DSM IP Assets B.V. Verfahren zur herstellung einer steviolglycosidzusammensetzung
CN107922913B (zh) 2015-08-13 2022-04-05 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷转运
EP3359654B1 (de) 2015-10-05 2022-01-05 DSM IP Assets B.V. Kaurensäurehydroxylasen
US11136600B2 (en) 2015-10-06 2021-10-05 Dsm Ip Assets B.V. Eukaryotic cell with increased production of fermentation product
WO2017158189A1 (en) 2016-03-18 2017-09-21 Lallemand Hungary Liquidity Management Llc Regulation of the ras/camp/pka signaling pathway in yeasts
CA3026325A1 (en) 2016-06-09 2017-12-14 Dsm Ip Assets B.V. Seed train for large scale enzyme production
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018029274A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
US11878992B2 (en) 2016-08-09 2024-01-23 Dsm Ip Assets B.V. Crystallization of steviol glycosides
CN109890961B (zh) 2016-10-27 2024-03-08 帝斯曼知识产权资产管理有限公司 香叶基香叶基焦磷酸酯合酶
CA3040380A1 (en) 2016-11-04 2018-05-11 Inbicon A/S Method for preparing fermentable sugars from lignocellulosic biomass
CA3043966A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme composition
BR112019009796A2 (pt) 2016-11-24 2019-08-06 Dsm Ip Assets Bv composição de enzimas
WO2018104238A1 (en) 2016-12-08 2018-06-14 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
FR3062134B1 (fr) 2017-01-24 2023-07-21 Lesaffre & Cie Obtention de souches de levure performantes pour la metabolisation de l'arabinose
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2018220116A1 (en) 2017-05-31 2018-12-06 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
WO2019002264A1 (en) 2017-06-27 2019-01-03 Dsm Ip Assets B.V. UDP-glycosyltransferase
DK3695001T3 (da) 2017-10-09 2024-01-29 Versalis Spa Fremgangsmåde til enzymatisk hydrolyse af lignocellulosemateriale og fermentering af sukker
CN111278986A (zh) 2017-10-30 2020-06-12 帝斯曼知识产权资产管理有限公司 用于酶促水解木质纤维素材料和发酵糖的方法
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019096718A1 (en) 2017-11-14 2019-05-23 Dsm Ip Assets B.V. Polypeptides with improved arabinose transport specificity
US11193145B2 (en) 2018-03-28 2021-12-07 Dsm Ip Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
WO2019219804A1 (en) 2018-05-17 2019-11-21 Dsm Ip Assets B.V. Process for producing a polypeptide
ES2944736T3 (es) 2018-05-30 2023-06-23 Versalis Spa Proceso para producir azúcares a partir de materiales de carbohidratos
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
BR112021017167A2 (pt) 2019-03-12 2021-11-09 Dsm Ip Assets Bv Processo para a produção de um caldo de fermentação
BR112022002942A2 (pt) 2019-09-10 2022-05-10 Dsm Ip Assets Bv Composição enzimática
US20220380813A1 (en) 2019-11-08 2022-12-01 Dsm Ip Assets B.V. Process for producing ethanol
EP4073089A1 (de) 2019-12-10 2022-10-19 Novozymes A/S Mikroorganismus zur verbesserten pentosefermentation
CA3198626A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
CN117178060A (zh) 2021-04-06 2023-12-05 帝斯曼知识产权资产管理有限公司 酶组合物
CA3214435A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
WO2022214459A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
AU2022253636A1 (en) 2021-04-08 2023-06-01 Versalis S.P.A. Process for the preparation of a sugar product and a fermentation product
CA3222371A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843760A (en) * 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8841090B2 (en) 2006-06-01 2014-09-23 Alliance For Sustainable Energy, Llc L-arabinose fermenting yeast
US7846712B2 (en) 2006-06-01 2010-12-07 Alliance For Sustainable Energy, Llc L-arabinose fermenting yeast
WO2007143245A3 (en) * 2006-06-01 2008-02-07 Midwest Research Inst An l-arabinose fermenting yeast
US8372626B2 (en) 2006-06-01 2013-02-12 Alliance For Sustainable Energy, Llc L-arabinose fermenting yeast
US20100144041A1 (en) * 2006-06-01 2010-06-10 Midwest Research Institute L-Arabinose Fermenting Yeast
WO2007143245A2 (en) * 2006-06-01 2007-12-13 Midwest Research Institute An l-arabinose fermenting yeast
WO2007143247A3 (en) * 2006-06-02 2008-02-21 Midwest Research Inst Cloning and characterization of l-arabinose transporters from non-conventional yeast
WO2007143247A2 (en) * 2006-06-02 2007-12-13 Midwest Research Institute Cloning and characterization of l-arabinose transporters from non-conventional yeast
US20100151548A1 (en) * 2007-04-05 2010-06-17 Eckhard Boles vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production
US8993301B2 (en) 2007-04-05 2015-03-31 Butalco Gmbh Vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production
US8753862B2 (en) * 2007-04-05 2014-06-17 Butalco Gmbh Vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production
US10947515B2 (en) 2015-03-16 2021-03-16 Dsm Ip Assets B.V. UDP-glycosyltransferases
US11459548B2 (en) 2015-03-16 2022-10-04 Dsm Ip Assets B.V. UDP-glycosyltransferases
WO2017176875A1 (en) 2016-04-08 2017-10-12 E I Du Pont De Nemours And Company Arabinose isomerases for yeast

Also Published As

Publication number Publication date
US20060270008A1 (en) 2006-11-30
US20090215138A1 (en) 2009-08-27
DK1499708T3 (da) 2006-05-22
DE60303127D1 (de) 2006-03-30
EP1499708B1 (de) 2006-01-04
ES2256742T5 (es) 2012-06-05
CA2483997C (en) 2013-04-02
CA2483997A1 (en) 2003-11-20
AU2003228189A1 (en) 2003-11-11
SE0202090D0 (sv) 2002-07-04
EP1499708A1 (de) 2005-01-26
ZA200408440B (en) 2005-07-27
WO2003095627A1 (en) 2003-11-20
ES2256742T3 (es) 2006-07-16
BRPI0309836B1 (pt) 2019-08-27
DE60303127T3 (de) 2012-07-19
PT1499708E (pt) 2006-05-31
DE60303127T2 (de) 2006-09-14
DK1499708T4 (da) 2012-05-07
EP1499708B2 (de) 2012-02-22
BR0309836A (pt) 2005-03-01
ATE315079T1 (de) 2006-02-15
US8691554B2 (en) 2014-04-08

Similar Documents

Publication Publication Date Title
EP1499708B2 (de) L-arabinose verbrauchende modifizierte hefe
US11753659B2 (en) Glycerol and acetic acid converting yeast cells with improved acetic acid conversion
CA2106377C (en) Ethanol production by recombinant hosts
Dien et al. Conversion of corn milling fibrous co-products into ethanol by recombinant Escherichia coli strains K011 and SL40
CN105121637A (zh) 酿酒酵母中替代甘油形成的消耗电子的乙醇生产途径
JP2013524796A (ja) 混合糖組成物の発酵に適した細胞
US20210310013A1 (en) Acetate consuming yeast cell
JP2014512818A (ja) アラビノース及びキシロースを含む糖類の変換能を有する酵母細胞
JP6048980B2 (ja) ペントース糖発酵細胞
Li et al. Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.
WO2014180820A2 (en) Gpd- yeast strains with improved osmotolerance
US10844363B2 (en) Xylose isomerase-modified yeast strains and methods for bioproduct production
US11692187B2 (en) Xylose isomerases that confer efficient xylose fermentation capability to yeast
US11702679B2 (en) Mutant gene associated with improvement in ethanol productivity via ethanol fermentation and method for producing ethanol using the same
JP6249391B2 (ja) キシロースを高温で発酵する方法
JP7298673B2 (ja) エタノール発酵によるエタノール生産性の向上に関与する変異遺伝子及びこれを用いたエタノールの製造方法
JP7298674B2 (ja) エタノール発酵によるエタノール生産性の向上に関与する変異遺伝子及びこれを用いたエタノールの製造方法
US20070259407A1 (en) Enzyme for an in Vivo and in Vitro Utilisation of Carbohydrates

Legal Events

Date Code Title Description
AS Assignment

Owner name: FORSKARPATENT I SYD AB, SWEDEN

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BOLES, ECKHARD;BECKER, JESSICA;REEL/FRAME:016358/0159

Effective date: 20040811

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION