WO2007143247A3 - Cloning and characterization of l-arabinose transporters from non-conventional yeast - Google Patents

Cloning and characterization of l-arabinose transporters from non-conventional yeast Download PDF

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Publication number
WO2007143247A3
WO2007143247A3 PCT/US2007/064418 US2007064418W WO2007143247A3 WO 2007143247 A3 WO2007143247 A3 WO 2007143247A3 US 2007064418 W US2007064418 W US 2007064418W WO 2007143247 A3 WO2007143247 A3 WO 2007143247A3
Authority
WO
WIPO (PCT)
Prior art keywords
arabinose
cloning
characterization
conventional yeast
transporters
Prior art date
Application number
PCT/US2007/064418
Other languages
French (fr)
Other versions
WO2007143247A2 (en
Inventor
Eric Knoshaug
Eric Jarvis
Arjun Singh
Mary Ann Franden
Min Zhang
Original Assignee
Midwest Research Inst
Eric Knoshaug
Eric Jarvis
Arjun Singh
Mary Ann Franden
Min Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Midwest Research Inst, Eric Knoshaug, Eric Jarvis, Arjun Singh, Mary Ann Franden, Min Zhang filed Critical Midwest Research Inst
Publication of WO2007143247A2 publication Critical patent/WO2007143247A2/en
Publication of WO2007143247A3 publication Critical patent/WO2007143247A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts

Abstract

Two genes from non-conventional yeast encode arabinose transporters. These arabinose transporters are capable of transporting arabinose across the cell membrane. These genes may be expressed heterologously in a host that is not otherwise capable of taking up significant amounts of arabinose from the environment of use. Methods are disclosed to use such genetically engineered hosts to ferment pentose such as arabinose, to produce ethanol.
PCT/US2007/064418 2006-06-02 2007-03-20 Cloning and characterization of l-arabinose transporters from non-conventional yeast WO2007143247A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81027406P 2006-06-02 2006-06-02
US60/810,274 2006-06-02

Publications (2)

Publication Number Publication Date
WO2007143247A2 WO2007143247A2 (en) 2007-12-13
WO2007143247A3 true WO2007143247A3 (en) 2008-02-21

Family

ID=38802155

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/064418 WO2007143247A2 (en) 2006-06-02 2007-03-20 Cloning and characterization of l-arabinose transporters from non-conventional yeast

Country Status (1)

Country Link
WO (1) WO2007143247A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006060381B4 (en) * 2006-12-20 2010-04-08 Johann Wolfgang Goethe-Universität Frankfurt am Main New specific arabinose transporter from the yeast Pichia stipitis and its uses
PT103780A (en) * 2007-07-06 2009-01-06 Univ Nova De Lisboa SEQUENCE OF DNA CODING A SPECIFIC TRANSPORTER FOR L-ARABINOSE, CDNA MOLECULE, PLASMIDE, UNDERSTANDING THE AFFECTED DNA SEQUENCE, HOST CELL TRANSFORMED WITH THAT PLUMBING AND APPLICATION.
AU2020402990A1 (en) * 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050142648A1 (en) * 2002-05-08 2005-06-30 Eckhard Boles Modified yeast consuming L-arabinose
US20050260705A1 (en) * 2000-03-24 2005-11-24 Better Marc D Methods and cells for expression of recombinant protein products under the transcriptional control of an inducible promoter

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050260705A1 (en) * 2000-03-24 2005-11-24 Better Marc D Methods and cells for expression of recombinant protein products under the transcriptional control of an inducible promoter
US20050142648A1 (en) * 2002-05-08 2005-06-30 Eckhard Boles Modified yeast consuming L-arabinose

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BILLARD ET AL.: "Glucose uptake in Kluyveromyces lactis: role of the HGT1 gene in glucose transport", J BACTERIOL., vol. 178, no. 20, October 1996 (1996-10-01), pages 5860 - 5866, XP002667185 *
SAHA ET AL.: "Production of L-arabitol from L-arabinose by Candida entomaea and Pichia guililiermondii", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 45, no. 3, April 1996 (1996-04-01), pages 299 - 306, XP000934273, DOI: doi:10.1007/s002530050687 *

Also Published As

Publication number Publication date
WO2007143247A2 (en) 2007-12-13

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