WO2012009272A3 - Pentose fermentation by a recombinant microorganism - Google Patents

Pentose fermentation by a recombinant microorganism Download PDF

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Publication number
WO2012009272A3
WO2012009272A3 PCT/US2011/043548 US2011043548W WO2012009272A3 WO 2012009272 A3 WO2012009272 A3 WO 2012009272A3 US 2011043548 W US2011043548 W US 2011043548W WO 2012009272 A3 WO2012009272 A3 WO 2012009272A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant microorganism
pentose fermentation
recombinant
xylose isomerase
pentose
Prior art date
Application number
PCT/US2011/043548
Other languages
French (fr)
Other versions
WO2012009272A2 (en
Inventor
Ezhilkani Subbian
Xiyun Zhang
Original Assignee
Codexis, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis, Inc. filed Critical Codexis, Inc.
Publication of WO2012009272A2 publication Critical patent/WO2012009272A2/en
Publication of WO2012009272A3 publication Critical patent/WO2012009272A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
PCT/US2011/043548 2010-07-14 2011-07-11 Pentose fermentation by a recombinant microorganism WO2012009272A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36418010P 2010-07-14 2010-07-14
US61/364,180 2010-07-14

Publications (2)

Publication Number Publication Date
WO2012009272A2 WO2012009272A2 (en) 2012-01-19
WO2012009272A3 true WO2012009272A3 (en) 2012-03-01

Family

ID=45470018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/043548 WO2012009272A2 (en) 2010-07-14 2011-07-11 Pentose fermentation by a recombinant microorganism

Country Status (1)

Country Link
WO (1) WO2012009272A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2626423A1 (en) * 2012-02-07 2013-08-14 Clariant Produkte (Deutschland) GmbH Pentose-fermenting microorganism
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
US8669076B1 (en) 2013-03-11 2014-03-11 E I Du Pont De Nemours And Company Cow rumen xylose isomerases active in yeast cells
BR112018000690A2 (en) 2015-07-13 2018-09-18 MARA Renewables Corporation recombinant microorganism, and methods for preparing a xylose metabolizing microorganism and for producing oil.
BR112019011612A2 (en) 2016-12-06 2020-08-18 Novozymes A/S improved processes for the production of ethanol from cellulosic substrates containing xylose using genetically modified yeast strains
CN110114465A (en) 2016-12-20 2019-08-09 诺维信公司 Restructuring yeast strains for pentose fermentation
AU2018275794A1 (en) 2017-06-02 2019-12-05 Novozymes A/S Improved yeast for ethanol production
CN113286871A (en) 2018-01-29 2021-08-20 诺维信公司 Microorganisms with enhanced nitrogen utilization for ethanol production
CN112601818A (en) 2018-07-25 2021-04-02 诺维信公司 Enzyme-expressing yeast for producing ethanol
BR112021006692A2 (en) 2018-10-08 2021-08-10 Novozymes A/S yeast expressing enzymes for ethanol production
BR112021026761A2 (en) 2019-07-26 2022-02-15 Novozymes As Yeast cell, composition, and methods of producing a derivative of a yeast strain, producing ethanol, and producing a fermentation product from a starch-containing or cellulose-containing material
EP4010469A1 (en) 2019-08-06 2022-06-15 Novozymes A/S Fusion proteins for improved enzyme expression
WO2021119304A1 (en) 2019-12-10 2021-06-17 Novozymes A/S Microorganism for improved pentose fermentation
CA3222371A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010000464A1 (en) * 2008-07-02 2010-01-07 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotic xylose isomerase for the construction of xylose fermenting yeasts
US20100086965A1 (en) * 2006-10-02 2010-04-08 Van Maris Antonius Jeroen Adriaan Metabolic engineering of arabinose-fermenting yeast cells
US20100129885A1 (en) * 2007-10-26 2010-05-27 Arbor Fuel Inc. Methods for the production of n-butanol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100086965A1 (en) * 2006-10-02 2010-04-08 Van Maris Antonius Jeroen Adriaan Metabolic engineering of arabinose-fermenting yeast cells
US20100129885A1 (en) * 2007-10-26 2010-05-27 Arbor Fuel Inc. Methods for the production of n-butanol
WO2010000464A1 (en) * 2008-07-02 2010-01-07 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotic xylose isomerase for the construction of xylose fermenting yeasts

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRAT ET AL.: "Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae.", APPL. ENV. MICRO., vol. 75, no. 8, April 2009 (2009-04-01), pages 2304 - 2311, XP009121860, DOI: doi:10.1128/AEM.02522-08 *
DATABASE UNIPROTKB/TREMBL [online] 7 July 2009 (2009-07-07), WEINSTOCK ET AL., retrieved from http://www.uniprot.org/uniprot/C4G751 .txt?version=8 Database accession no. C4G751 *

Also Published As

Publication number Publication date
WO2012009272A2 (en) 2012-01-19

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