US10077286B2 - Method for removal of triglycerides in lipoproteins other than low-density lipoproteins - Google Patents

Method for removal of triglycerides in lipoproteins other than low-density lipoproteins Download PDF

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US10077286B2
US10077286B2 US14/395,449 US201314395449A US10077286B2 US 10077286 B2 US10077286 B2 US 10077286B2 US 201314395449 A US201314395449 A US 201314395449A US 10077286 B2 US10077286 B2 US 10077286B2
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triglycerides
ldl
reagent
lipoproteins
surfactant
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US20150132834A1 (en
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Motoko Ohta
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Denka Seiken Co Ltd
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Denka Seiken Co Ltd
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Assigned to DENKA COMPANY LIMITED reassignment DENKA COMPANY LIMITED CORRECTIVE ASSIGNMENT TO CORRECT THE PATENT NUMBERS 10007286 AND 10415007 PREVIOUSLY RECORDED AT REEL: 054513 FRAME: 0142. ASSIGNOR(S) HEREBY CONFIRMS THE MERGER. Assignors: DENKA SEIKEN CO., LTD.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/32—Cardiovascular disorders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7057—(Intracellular) signaling and trafficking pathways
    • G01N2800/7066—Metabolic pathways
    • G01N2800/7085—Lipogenesis or lipolysis, e.g. fatty acid metabolism

Definitions

  • Preferred examples of the cholesterol esterase to be used in the method of the present invention include cholesterol esterase having a molecular weight of not more than 50 kDa. Even in cases where a cholesterol esterase has a molecular weight of not less than 50 kDa, the cholesterol esterase is preferred if the cholesterol esterase has a subunit with a molecular weight of not more than 50 kDa.
  • the origin of the cholesterol esterase is not limited, and may be derived from any animal or microorganism. A cholesterol esterase derived from Pseudomonas sp. may be especially preferably used.
  • the LDL-TG quantification reagents having the following compositions were prepared.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
US14/395,449 2012-04-20 2013-04-19 Method for removal of triglycerides in lipoproteins other than low-density lipoproteins Active US10077286B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012097161 2012-04-20
JP2012-097161 2012-04-20
PCT/JP2013/061672 WO2013157642A1 (ja) 2012-04-20 2013-04-19 低密度リポ蛋白以外のリポ蛋白中のトリグリセリドの消去方法

Publications (2)

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US20150132834A1 US20150132834A1 (en) 2015-05-14
US10077286B2 true US10077286B2 (en) 2018-09-18

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US14/395,449 Active US10077286B2 (en) 2012-04-20 2013-04-19 Method for removal of triglycerides in lipoproteins other than low-density lipoproteins

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US (1) US10077286B2 (zh)
EP (1) EP2840145B8 (zh)
JP (1) JP6240065B2 (zh)
KR (1) KR102068515B1 (zh)
CN (1) CN104583419B (zh)
TW (1) TWI507671B (zh)
WO (1) WO2013157642A1 (zh)

Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
JP6967857B2 (ja) * 2017-02-03 2021-11-17 デンカ株式会社 循環器疾患等のリスクの判断を補助する方法
CN106950095B (zh) * 2017-04-01 2020-01-10 合肥迪安医学检验所有限公司 一种减少脂血样本检测干扰的方法
CN108949903B (zh) * 2017-05-17 2021-11-16 广州市伊川生物科技有限公司 一种甘油三酯测定试剂盒及其测定方法
US11513131B2 (en) * 2018-01-22 2022-11-29 Global Genomics Group, LLC Low density lipoprotein triglycerides (LDL-TG) as a biomarker of cardiovascular disease and uses thereof
US20200400694A1 (en) * 2018-03-13 2020-12-24 Denka Company Limited Method for assisting determination of risk of cardiovascular disease or the like
JP7029139B2 (ja) 2018-08-23 2022-03-03 デンカ株式会社 非アルコール性脂肪性肝炎の検出を補助する方法
WO2021157631A1 (ja) 2020-02-04 2021-08-12 デンカ株式会社 非アルコール性脂肪肝炎の検出を補助する方法
EP3865874B1 (en) * 2020-02-13 2022-10-05 Zhejiang Orient Gene Biotech Co., LTD Distinguishing smoking e-cigarettes from smoking cigarettes

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3645688A (en) * 1970-03-25 1972-02-29 Oxford Lab Measuring triglyceride and cholesterol in blood plasma or serum
WO2000043537A1 (fr) 1999-01-20 2000-07-27 Kyowa Medex Co., Ltd. Methode pour mesurer un triglyceride dans une lipoproteine
WO2000052480A1 (fr) 1999-03-01 2000-09-08 International Reagents Corporation Dosage de composant d'un echantillon biologique
WO2003104486A1 (ja) 2002-06-10 2003-12-18 株式会社シノテスト トリグリセライドの選択的測定方法
WO2004055204A1 (ja) 2002-12-13 2004-07-01 Denka Seiken Co., Ltd. 低密度リポ蛋白中コレステロールのマルチ定量法
JP2006180707A (ja) 2003-03-28 2006-07-13 Denka Seiken Co Ltd 低密度リポ蛋白中のトリグリセリドの定量方法
WO2007052646A1 (ja) 2005-10-31 2007-05-10 Kyowa Medex Co., Ltd. 低密度リポ蛋白中トリグリセリドの測定方法及び測定用キット
US7682831B2 (en) * 2002-11-27 2010-03-23 Sekisui Medical Co., Ltd. Method of measuring lipid in specific lipoprotein

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61104798A (ja) * 1984-10-25 1986-05-23 Toyobo Co Ltd 脂質測定用酵素含有組成物
JP2000043537A (ja) 1998-07-31 2000-02-15 Denso Corp 空気通路切替装置および車両用空調装置
WO2001094619A1 (fr) * 2000-06-07 2001-12-13 International Reagents Corporation Methode d'analyse de composants dans des echantillons biologiques
CA2589459C (en) * 2004-11-29 2014-01-07 Jimro Co., Ltd. Method of measuring cholesterol in remnant-like lipoproteins

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3645688A (en) * 1970-03-25 1972-02-29 Oxford Lab Measuring triglyceride and cholesterol in blood plasma or serum
WO2000043537A1 (fr) 1999-01-20 2000-07-27 Kyowa Medex Co., Ltd. Methode pour mesurer un triglyceride dans une lipoproteine
US6811994B1 (en) 1999-01-20 2004-11-02 Kyowa Medex Co., Ltd. Method for quantitating triglycerides in lipoproteins
US20040161811A1 (en) 1999-03-01 2004-08-19 Koji Kishi Reagent kit for detecting cholesterol in a high-density lipoprotein
WO2000052480A1 (fr) 1999-03-01 2000-09-08 International Reagents Corporation Dosage de composant d'un echantillon biologique
WO2003104486A1 (ja) 2002-06-10 2003-12-18 株式会社シノテスト トリグリセライドの選択的測定方法
US20050255536A1 (en) 2002-06-10 2005-11-17 Shino-Test Corporation Method of selectively measuring triglycerides
US7682831B2 (en) * 2002-11-27 2010-03-23 Sekisui Medical Co., Ltd. Method of measuring lipid in specific lipoprotein
WO2004055204A1 (ja) 2002-12-13 2004-07-01 Denka Seiken Co., Ltd. 低密度リポ蛋白中コレステロールのマルチ定量法
US20060078958A1 (en) 2002-12-13 2006-04-13 Hiroshi Matsui Method of multiple quantification of cholesterol in low-density lipoproteins
JP2006180707A (ja) 2003-03-28 2006-07-13 Denka Seiken Co Ltd 低密度リポ蛋白中のトリグリセリドの定量方法
WO2007052646A1 (ja) 2005-10-31 2007-05-10 Kyowa Medex Co., Ltd. 低密度リポ蛋白中トリグリセリドの測定方法及び測定用キット
US20090226944A1 (en) 2005-10-31 2009-09-10 Kyowa Medex Co., Ltd. Method for measuring triglyceride in low-density lipoprotein and kit for measurement

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Cholesterol esterase data sheet, 2007, 1 page. *
Extended European Search Report issued in European Patent Application No. 13777616.7 dated Nov. 23, 2015.
International Search Report, issued in PCT/JP2013/061672, dated Jul. 9, 2013.

Also Published As

Publication number Publication date
EP2840145A1 (en) 2015-02-25
KR20150005625A (ko) 2015-01-14
TWI507671B (zh) 2015-11-11
CN104583419B (zh) 2017-12-19
EP2840145B8 (en) 2020-10-28
KR102068515B1 (ko) 2020-01-21
TW201348691A (zh) 2013-12-01
EP2840145B1 (en) 2020-09-02
JP6240065B2 (ja) 2017-11-29
EP2840145A4 (en) 2015-12-23
JPWO2013157642A1 (ja) 2015-12-21
US20150132834A1 (en) 2015-05-14
CN104583419A (zh) 2015-04-29
WO2013157642A1 (ja) 2013-10-24

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