TWI670004B - 用來產生植物之螢光激活細胞分選富增技術 - Google Patents

用來產生植物之螢光激活細胞分選富增技術 Download PDF

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TWI670004B
TWI670004B TW102132254A TW102132254A TWI670004B TW I670004 B TWI670004 B TW I670004B TW 102132254 A TW102132254 A TW 102132254A TW 102132254 A TW102132254 A TW 102132254A TW I670004 B TWI670004 B TW I670004B
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dna
artificial sequence
plant
sequence
gene
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TW102132254A
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TW201424578A (zh
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吉爾曼 史賓根堡
薩瑞納 塞哈卜
約翰 梅森
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美商陶氏農業科學公司
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Dispersion Chemistry (AREA)
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  • Immunology (AREA)
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  • Botany (AREA)
  • Physiology (AREA)
TW102132254A 2012-09-07 2013-09-06 用來產生植物之螢光激活細胞分選富增技術 TWI670004B (zh)

Applications Claiming Priority (2)

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US201261697890P 2012-09-07 2012-09-07
US61/697,890 2012-09-07

Publications (2)

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TW201424578A TW201424578A (zh) 2014-07-01
TWI670004B true TWI670004B (zh) 2019-09-01

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US (1) US20140075593A1 (https=)
EP (1) EP2893024B1 (https=)
JP (1) JP6362600B2 (https=)
KR (1) KR102219621B1 (https=)
CN (2) CN111118100A (https=)
AR (1) AR092482A1 (https=)
AU (1) AU2013312198B2 (https=)
BR (1) BR112015004864B8 (https=)
CA (1) CA2883800C (https=)
HK (1) HK1210220A1 (https=)
IL (1) IL237535B (https=)
IN (1) IN2015DN01785A (https=)
MX (1) MX367170B (https=)
RU (1) RU2679510C2 (https=)
TW (1) TWI670004B (https=)
WO (1) WO2014039970A1 (https=)
ZA (1) ZA201501736B (https=)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
CA2884162C (en) * 2012-09-07 2020-12-29 Dow Agrosciences Llc Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10900034B2 (en) 2014-12-03 2021-01-26 Agilent Technologies, Inc. Guide RNA with chemical modifications
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したCas9蛋白質
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3510145A4 (en) 2016-09-06 2020-03-25 The Children's Medical Center Corporation IMMUNCELLS FROM INDUCED PLURIPOTENT STEM CELLS
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
EP3630973A1 (en) 2017-05-31 2020-04-08 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US20200270604A1 (en) 2017-09-19 2020-08-27 Tropic Biosciences UK Limited Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート,インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
GB201905542D0 (en) * 2019-04-18 2019-06-05 Phytoform Labs Ltd Methods, systems and apparatus for plant material screening and propagation
US20230051935A1 (en) * 2019-09-30 2023-02-16 Calyxt, Inc. Gene editing using a messenger ribonucleic acid construct
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
JP2024534945A (ja) 2021-09-10 2024-09-26 アジレント・テクノロジーズ・インク 化学修飾を有するプライム編集のためのガイドrna
CN117701591B (zh) * 2023-12-25 2024-11-19 西北农林科技大学 与不结球白菜基因共分离的叶柄厚分子标记t33、引物及应用
CN120082500B (zh) * 2025-04-28 2026-01-27 北京艾普希隆生物科技有限公司 一种制备植物单细胞的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200815593A (en) * 2006-08-11 2008-04-01 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4727028A (en) 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
ATE52800T1 (de) 1983-01-13 1990-06-15 Max Planck Gesellschaft Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
WO1984002919A1 (en) 1983-01-17 1984-08-02 Monsanto Co Plasmids for transforming plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
EP0265502A1 (en) 1986-04-30 1988-05-04 Boyce Thompson Institute For Plant Research, Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
ATE125418T1 (de) 1987-05-20 1995-08-15 Ciba Geigy Ag Zeamays-pflanzen und transgenetische zeamays- pflanzen, die aus protoplasten oder aus von protoplasten erhaltenen zellen regeneriert wurden.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
DE69133512T2 (de) 1990-11-23 2006-09-28 Bayer Bioscience N.V. Verfahren zur Transformation monokotyler Pflanzen
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
JPH05130861A (ja) * 1991-11-06 1993-05-28 Hitachi Chem Co Ltd プロトプラストからクローン細胞を作製する方法
JPH07505531A (ja) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ 単子葉植物細胞の形質転換法
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
DE69404385T2 (de) 1993-06-04 1998-02-19 Cavis Srl Trägheitsschalter zur Ausschaltung der Batterie eines Kraftfahrzeuges
CA2234656C (en) 1995-10-13 2008-01-08 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US20030219763A1 (en) * 1996-12-13 2003-11-27 Jen Sheen Plant protoplast gene expression systems and uses thereof
EP1159418A2 (en) 1998-12-11 2001-12-05 Biomira Inc. Muc-1 antagonists and methods of treating immune disorders
EP1130093A1 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1250447B1 (en) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
JP3780333B2 (ja) * 2000-09-22 2006-05-31 国立大学法人大阪大学 外来性遺伝物質又は生理活性物質を細胞内へ導入する新規な方法
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
HUE047016T2 (hu) 2004-03-26 2020-04-28 Dow Agrosciences Llc CRY1F és CRY1AC transzgenikus gyapotvonalak és eseményspecifikus azonosításuk
KR101520507B1 (ko) 2006-12-14 2015-05-29 다우 아그로사이언시즈 엘엘씨 최적화된 비-정규 아연 손가락 단백질
CA2700231C (en) 2007-09-27 2018-09-18 Sangamo Biosciences, Inc. Rapid in vivo identification of biologically active nucleases
EP2455454A1 (en) * 2010-11-19 2012-05-23 Albert-Ludwigs-Universität Freiburg Method for introducing a polynucleotide into plant protoplast cells
CN103596423B (zh) * 2011-06-07 2017-05-24 弗劳恩霍夫应用研究促进协会 产生单克隆植物细胞系的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200815593A (en) * 2006-08-11 2008-04-01 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Bastiaan O.R. et al., "Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts", Plant Physiology, March 2009, Vol. 149, pp. 1231–1239.
Bastiaan O.R. et al., "Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts", Plant Physiology, March 2009, Vol. 149, pp. 1231–1239. Kageyama, Y., et al.," Plant regeneration from patchouli protoplasts encapsulated in alginate beads", Plant Cell Tissue Organ Cult., 1995, Vol.41, P.65-70. *
Kageyama, Y., et al.," Plant regeneration from patchouli protoplasts encapsulated in alginate beads", Plant Cell Tissue Organ Cult., 1995, Vol.41, P.65-70.

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CN104736712A (zh) 2015-06-24
AR092482A1 (es) 2015-04-22
HK1210220A1 (en) 2016-04-15
RU2015112573A (ru) 2016-10-27
TW201424578A (zh) 2014-07-01
CA2883800A1 (en) 2014-03-13
US20140075593A1 (en) 2014-03-13
CN111118100A (zh) 2020-05-08
BR112015004864B8 (pt) 2023-05-16
EP2893024A4 (en) 2016-03-30
BR112015004864A2 (pt) 2017-11-21
IN2015DN01785A (https=) 2015-05-29
IL237535B (en) 2019-11-28
AU2013312198A1 (en) 2015-03-19
CA2883800C (en) 2021-07-13
JP6362600B2 (ja) 2018-07-25
MX367170B (es) 2019-08-07
BR112015004864B1 (pt) 2022-10-25
IL237535A0 (en) 2015-04-30
MX2015002964A (es) 2015-06-05
KR20150052241A (ko) 2015-05-13
EP2893024A1 (en) 2015-07-15
WO2014039970A1 (en) 2014-03-13
ZA201501736B (en) 2016-10-26
KR102219621B1 (ko) 2021-02-24
RU2679510C2 (ru) 2019-02-11
AU2013312198B2 (en) 2018-11-08
JP2015527085A (ja) 2015-09-17
WO2014039970A9 (en) 2014-10-09
EP2893024B1 (en) 2019-12-04

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