CA2883800C - Fluorescence activated cell sorting (facs) enrichment to generate plants - Google Patents
Fluorescence activated cell sorting (facs) enrichment to generate plants Download PDFInfo
- Publication number
- CA2883800C CA2883800C CA2883800A CA2883800A CA2883800C CA 2883800 C CA2883800 C CA 2883800C CA 2883800 A CA2883800 A CA 2883800A CA 2883800 A CA2883800 A CA 2883800A CA 2883800 C CA2883800 C CA 2883800C
- Authority
- CA
- Canada
- Prior art keywords
- seq
- plant
- etip
- protoplast
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261697890P | 2012-09-07 | 2012-09-07 | |
| US61/697,890 | 2012-09-07 | ||
| US14/020,694 | 2013-09-06 | ||
| US14/020,694 US20140075593A1 (en) | 2012-09-07 | 2013-09-06 | Fluorescence activated cell sorting (facs) enrichment to generate plants |
| PCT/US2013/058766 WO2014039970A1 (en) | 2012-09-07 | 2013-09-09 | Fluorescence activated cell sorting (facs) enrichment to generate plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2883800A1 CA2883800A1 (en) | 2014-03-13 |
| CA2883800C true CA2883800C (en) | 2021-07-13 |
Family
ID=50234835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2883800A Active CA2883800C (en) | 2012-09-07 | 2013-09-09 | Fluorescence activated cell sorting (facs) enrichment to generate plants |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20140075593A1 (https=) |
| EP (1) | EP2893024B1 (https=) |
| JP (1) | JP6362600B2 (https=) |
| KR (1) | KR102219621B1 (https=) |
| CN (2) | CN104736712A (https=) |
| AR (1) | AR092482A1 (https=) |
| AU (1) | AU2013312198B2 (https=) |
| BR (1) | BR112015004864B8 (https=) |
| CA (1) | CA2883800C (https=) |
| HK (1) | HK1210220A1 (https=) |
| IL (1) | IL237535B (https=) |
| IN (1) | IN2015DN01785A (https=) |
| MX (1) | MX367170B (https=) |
| RU (1) | RU2679510C2 (https=) |
| TW (1) | TWI670004B (https=) |
| WO (1) | WO2014039970A1 (https=) |
| ZA (1) | ZA201501736B (https=) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9914930B2 (en) * | 2012-09-07 | 2018-03-13 | Dow Agrosciences Llc | FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
| UA119135C2 (uk) | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| CA2969619A1 (en) | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| KR102888521B1 (ko) | 2015-04-06 | 2025-11-19 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| CN106198358B (zh) * | 2016-06-24 | 2019-02-15 | 北京大学 | 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法 |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11525119B2 (en) | 2016-09-06 | 2022-12-13 | The Children's Medical Center Corporation | Immune cells derived from induced pluripotent stem cell |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| GB201708662D0 (en) | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing shelf-life of banana |
| WO2018220582A1 (en) | 2017-05-31 | 2018-12-06 | Tropic Biosciences UK Limited | Methods of selecting cells comprising genome editing events |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| SG11202002481RA (en) | 2017-09-19 | 2020-04-29 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| GB201905542D0 (en) * | 2019-04-18 | 2019-06-05 | Phytoform Labs Ltd | Methods, systems and apparatus for plant material screening and propagation |
| WO2021067394A1 (en) * | 2019-09-30 | 2021-04-08 | Calyxt, Inc. | Gene editing using a messenger ribonucleic acid construct |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| JP2024534945A (ja) | 2021-09-10 | 2024-09-26 | アジレント・テクノロジーズ・インク | 化学修飾を有するプライム編集のためのガイドｒｎａ |
| CN117701591B (zh) * | 2023-12-25 | 2024-11-19 | 西北农林科技大学 | 与不结球白菜基因共分离的叶柄厚分子标记t33、引物及应用 |
| CN120082500B (zh) * | 2025-04-28 | 2026-01-27 | 北京艾普希隆生物科技有限公司 | 一种制备植物单细胞的方法 |
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-
2013
- 2013-09-06 US US14/020,694 patent/US20140075593A1/en not_active Abandoned
- 2013-09-06 AR ARP130103193A patent/AR092482A1/es unknown
- 2013-09-06 TW TW102132254A patent/TWI670004B/zh not_active IP Right Cessation
- 2013-09-09 CN CN201380054087.4A patent/CN104736712A/zh active Pending
- 2013-09-09 RU RU2015112573A patent/RU2679510C2/ru active
- 2013-09-09 KR KR1020157008557A patent/KR102219621B1/ko not_active Expired - Fee Related
- 2013-09-09 JP JP2015531282A patent/JP6362600B2/ja not_active Expired - Fee Related
- 2013-09-09 HK HK15111003.5A patent/HK1210220A1/xx unknown
- 2013-09-09 CN CN201910796679.2A patent/CN111118100A/zh active Pending
- 2013-09-09 CA CA2883800A patent/CA2883800C/en active Active
- 2013-09-09 EP EP13835630.8A patent/EP2893024B1/en active Active
- 2013-09-09 BR BR112015004864A patent/BR112015004864B8/pt active IP Right Grant
- 2013-09-09 MX MX2015002964A patent/MX367170B/es active IP Right Grant
- 2013-09-09 WO PCT/US2013/058766 patent/WO2014039970A1/en not_active Ceased
- 2013-09-09 AU AU2013312198A patent/AU2013312198B2/en not_active Ceased
- 2013-09-09 IN IN1785DEN2015 patent/IN2015DN01785A/en unknown
-
2015
- 2015-03-03 IL IL23753515A patent/IL237535B/en active IP Right Grant
- 2015-03-13 ZA ZA2015/01736A patent/ZA201501736B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014039970A1 (en) | 2014-03-13 |
| KR102219621B1 (ko) | 2021-02-24 |
| RU2679510C2 (ru) | 2019-02-11 |
| EP2893024A4 (en) | 2016-03-30 |
| JP6362600B2 (ja) | 2018-07-25 |
| TWI670004B (zh) | 2019-09-01 |
| CN111118100A (zh) | 2020-05-08 |
| CN104736712A (zh) | 2015-06-24 |
| IL237535A0 (en) | 2015-04-30 |
| RU2015112573A (ru) | 2016-10-27 |
| BR112015004864A2 (pt) | 2017-11-21 |
| IL237535B (en) | 2019-11-28 |
| ZA201501736B (en) | 2016-10-26 |
| KR20150052241A (ko) | 2015-05-13 |
| EP2893024B1 (en) | 2019-12-04 |
| BR112015004864B8 (pt) | 2023-05-16 |
| MX367170B (es) | 2019-08-07 |
| CA2883800A1 (en) | 2014-03-13 |
| TW201424578A (zh) | 2014-07-01 |
| WO2014039970A9 (en) | 2014-10-09 |
| US20140075593A1 (en) | 2014-03-13 |
| AR092482A1 (es) | 2015-04-22 |
| EP2893024A1 (en) | 2015-07-15 |
| AU2013312198A1 (en) | 2015-03-19 |
| AU2013312198B2 (en) | 2018-11-08 |
| HK1210220A1 (en) | 2016-04-15 |
| BR112015004864B1 (pt) | 2022-10-25 |
| JP2015527085A (ja) | 2015-09-17 |
| MX2015002964A (es) | 2015-06-05 |
| IN2015DN01785A (https=) | 2015-05-29 |
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