TWI670004B - 用來產生植物之螢光激活細胞分選富增技術 - Google Patents

用來產生植物之螢光激活細胞分選富增技術 Download PDF

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TWI670004B
TWI670004B TW102132254A TW102132254A TWI670004B TW I670004 B TWI670004 B TW I670004B TW 102132254 A TW102132254 A TW 102132254A TW 102132254 A TW102132254 A TW 102132254A TW I670004 B TWI670004 B TW I670004B
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dna
artificial sequence
plant
sequence
gene
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TW102132254A
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TW201424578A (zh
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吉爾曼 史賓根堡
薩瑞納 塞哈卜
約翰 梅森
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美商陶氏農業科學公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
TW102132254A 2012-09-07 2013-09-06 用來產生植物之螢光激活細胞分選富增技術 TWI670004B (zh)

Applications Claiming Priority (2)

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US201261697890P 2012-09-07 2012-09-07
US61/697,890 2012-09-07

Publications (2)

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TW201424578A TW201424578A (zh) 2014-07-01
TWI670004B true TWI670004B (zh) 2019-09-01

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TW102132254A TWI670004B (zh) 2012-09-07 2013-09-06 用來產生植物之螢光激活細胞分選富增技術

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US (1) US20140075593A1 (cg-RX-API-DMAC7.html)
EP (1) EP2893024B1 (cg-RX-API-DMAC7.html)
JP (1) JP6362600B2 (cg-RX-API-DMAC7.html)
KR (1) KR102219621B1 (cg-RX-API-DMAC7.html)
CN (2) CN111118100A (cg-RX-API-DMAC7.html)
AR (1) AR092482A1 (cg-RX-API-DMAC7.html)
AU (1) AU2013312198B2 (cg-RX-API-DMAC7.html)
BR (1) BR112015004864B8 (cg-RX-API-DMAC7.html)
CA (1) CA2883800C (cg-RX-API-DMAC7.html)
HK (1) HK1210220A1 (cg-RX-API-DMAC7.html)
IL (1) IL237535B (cg-RX-API-DMAC7.html)
IN (1) IN2015DN01785A (cg-RX-API-DMAC7.html)
MX (1) MX367170B (cg-RX-API-DMAC7.html)
RU (1) RU2679510C2 (cg-RX-API-DMAC7.html)
TW (1) TWI670004B (cg-RX-API-DMAC7.html)
WO (1) WO2014039970A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201501736B (cg-RX-API-DMAC7.html)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
EP3406715B1 (en) * 2012-09-07 2023-12-13 Corteva Agriscience LLC Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
CA2969619A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
CA2981715A1 (en) 2015-04-06 2016-10-13 The Board Of Trustees Of The Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
KR102827276B1 (ko) 2016-08-03 2025-07-01 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
GB2575930A (en) 2017-03-23 2020-01-29 Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
US20200109408A1 (en) 2017-05-31 2020-04-09 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
GB201905542D0 (en) * 2019-04-18 2019-06-05 Phytoform Labs Ltd Methods, systems and apparatus for plant material screening and propagation
EP4022055A4 (en) * 2019-09-30 2024-01-03 Calyxt, Inc. GENE EDITING USING A MESSENGER RIBONUCLEIC ACID CONSTRUCTION
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
AU2021267940A1 (en) 2020-05-08 2022-12-08 President And Fellows Of Harvard College Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
CN117701591B (zh) * 2023-12-25 2024-11-19 西北农林科技大学 与不结球白菜基因共分离的叶柄厚分子标记t33、引物及应用
CN120082500A (zh) * 2025-04-28 2025-06-03 北京艾普希隆生物科技有限公司 一种制备植物单细胞的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200815593A (en) * 2006-08-11 2008-04-01 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4727028A (en) 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0116718B2 (en) 1983-01-13 1996-05-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Process for the introduction of expressible genes into plant cell genomes and agrobacterium strains carrying hybrid Ti plasmid vectors useful for this process
JPS60500438A (ja) 1983-01-17 1985-04-04 モンサント　カンパニ− 植物細胞を形質転換するためのプラスミド
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1987006614A1 (en) 1986-04-30 1987-11-05 Boyce Thompson Institute For Plant Research, Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
ES2121803T3 (es) 1987-05-20 1998-12-16 Novartis Ag Plantas de zea mays y plantas transgenicas de zea mays generadas a partir de protoplastos o celulas derivadas de protoplastos.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
DE69132124T2 (de) 1990-11-23 2000-11-23 Aventis Cropscience N.V., Gent Verfahren zur transformation monokotyler pflanzen
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
JPH05130861A (ja) * 1991-11-06 1993-05-28 Hitachi Chem Co Ltd プロトプラストからクローン細胞を作製する方法
US5679558A (en) 1992-04-15 1997-10-21 Plant Genetic Systems, N.V. Transformation of monocot cells
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
ES2105415T3 (es) 1993-06-04 1997-10-16 Cavis Srl Dispositivo de inercia para interrumpir el circuito electrico de un vehiculo con un motor de combustion interna.
BR9611000A (pt) 1995-10-13 1999-12-28 Dow Agro Sciences Llc Seq³ência de nucleotídeos otimizada de plantas e de gene inseticida otimizada específica do milho, construção genética sintética capaz de expressão em células de plantas, planta transgênica, somente de plantas, processo de construção de uma seq³ência de gene inseticida otimizada específica de milho e promotor recombinante.
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US20030219763A1 (en) * 1996-12-13 2003-11-27 Jen Sheen Plant protoplast gene expression systems and uses thereof
AU2354700A (en) 1998-12-11 2000-06-26 Biomira Inc. Muc-1 antagonists and methods of treating immune disorders
EP1108781A3 (en) 1999-01-19 2001-09-12 Maxygen, Inc. Method for making character strings, polynucleotides and polypeptides
BR0016030A (pt) 1999-11-29 2002-07-30 Midwest Oilseeds Inc Métodos e composições para a introdução de moléculas em células
JP3780333B2 (ja) * 2000-09-22 2006-05-31 国立大学法人大阪大学 外来性遺伝物質又は生理活性物質を細胞内へ導入する新規な方法
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
EP2368982A3 (en) 2002-03-21 2011-10-12 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
EP1737964A1 (en) 2004-03-26 2007-01-03 Dow AgroSciences LLC Cry1f and cry1ac transgenic cotton lines and event-specific identification thereof
PT2415873E (pt) 2006-12-14 2015-03-31 Sangamo Biosciences Inc Proteínas com dedos de zinco não canónicas optimizadas
JP5763341B2 (ja) 2007-09-27 2015-08-12 サンガモ バイオサイエンシーズ， インコーポレイテッド 生物学的活性のあるヌクレアーゼの迅速なインビボ同定法
EP2455454A1 (en) * 2010-11-19 2012-05-23 Albert-Ludwigs-Universität Freiburg Method for introducing a polynucleotide into plant protoplast cells
RU2573927C2 (ru) * 2011-06-07 2016-01-27 Фраунхофер-Гезелльшафт Цур Фердерунг Дер Ангевандтен Форшунг Е.Ф. Способ получения моноклональной линии растительных клеток

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200815593A (en) * 2006-08-11 2008-04-01 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Bastiaan O.R. et al., "Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts", Plant Physiology, March 2009, Vol. 149, pp. 1231–1239.
Bastiaan O.R. et al., "Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts", Plant Physiology, March 2009, Vol. 149, pp. 1231–1239. Kageyama, Y., et al.," Plant regeneration from patchouli protoplasts encapsulated in alginate beads", Plant Cell Tissue Organ Cult., 1995, Vol.41, P.65-70. *
Kageyama, Y., et al.," Plant regeneration from patchouli protoplasts encapsulated in alginate beads", Plant Cell Tissue Organ Cult., 1995, Vol.41, P.65-70.

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KR102219621B1 (ko) 2021-02-24
MX367170B (es) 2019-08-07
JP6362600B2 (ja) 2018-07-25
BR112015004864A2 (pt) 2017-11-21
US20140075593A1 (en) 2014-03-13
BR112015004864B8 (pt) 2023-05-16
RU2015112573A (ru) 2016-10-27
AU2013312198A1 (en) 2015-03-19
RU2679510C2 (ru) 2019-02-11
ZA201501736B (en) 2016-10-26
TW201424578A (zh) 2014-07-01
CN104736712A (zh) 2015-06-24
CA2883800A1 (en) 2014-03-13
JP2015527085A (ja) 2015-09-17
CN111118100A (zh) 2020-05-08
AR092482A1 (es) 2015-04-22
CA2883800C (en) 2021-07-13
EP2893024A1 (en) 2015-07-15
HK1210220A1 (en) 2016-04-15
WO2014039970A9 (en) 2014-10-09
BR112015004864B1 (pt) 2022-10-25
EP2893024A4 (en) 2016-03-30
IL237535A0 (en) 2015-04-30
WO2014039970A1 (en) 2014-03-13
IN2015DN01785A (cg-RX-API-DMAC7.html) 2015-05-29
KR20150052241A (ko) 2015-05-13
IL237535B (en) 2019-11-28
EP2893024B1 (en) 2019-12-04
MX2015002964A (es) 2015-06-05
AU2013312198B2 (en) 2018-11-08

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