TW546475B - A method for quantifying hydrogen sulfide or sulfide ion and a method for quantifying homocysteine or cysteine by using the method - Google Patents
A method for quantifying hydrogen sulfide or sulfide ion and a method for quantifying homocysteine or cysteine by using the method Download PDFInfo
- Publication number
- TW546475B TW546475B TW088120574A TW88120574A TW546475B TW 546475 B TW546475 B TW 546475B TW 088120574 A TW088120574 A TW 088120574A TW 88120574 A TW88120574 A TW 88120574A TW 546475 B TW546475 B TW 546475B
- Authority
- TW
- Taiwan
- Prior art keywords
- sulfide
- ions
- metal
- hydrogen sulfide
- color development
- Prior art date
Links
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 title abstract description 13
- 229910000037 hydrogen sulfide Inorganic materials 0.000 title abstract description 13
- 238000000034 method Methods 0.000 title abstract description 12
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 title description 10
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 title description 8
- 235000018417 cysteine Nutrition 0.000 title description 8
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 title description 6
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 title description 5
- -1 sulfide ions Chemical class 0.000 abstract description 23
- 239000002184 metal Substances 0.000 abstract description 15
- 229910052751 metal Inorganic materials 0.000 abstract description 15
- 229910021645 metal ion Inorganic materials 0.000 abstract description 14
- 150000001875 compounds Chemical class 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract 1
- 238000011002 quantification Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 108090000856 Lyases Proteins 0.000 description 4
- 102000004317 Lyases Human genes 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 3
- 108010076010 Cystathionine beta-lyase Proteins 0.000 description 2
- 108010083493 Cysteine lyase Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 description 2
- 229940099500 cystamine Drugs 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- BXRLWGXPSRYJDZ-VKHMYHEASA-N 3-cyano-L-alanine Chemical compound OC(=O)[C@@H](N)CC#N BXRLWGXPSRYJDZ-VKHMYHEASA-N 0.000 description 1
- GBOMJDKLSFIFFA-UHFFFAOYSA-N 4-hydrazinylbutanenitrile Chemical compound NNCCCC#N GBOMJDKLSFIFFA-UHFFFAOYSA-N 0.000 description 1
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 1
- 239000004201 L-cysteine Substances 0.000 description 1
- 235000013878 L-cysteine Nutrition 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241000282330 Procyon lotor Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- PJUIMOJAAPLTRJ-UHFFFAOYSA-N monothioglycerol Chemical compound OCC(O)CS PJUIMOJAAPLTRJ-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 229940035024 thioglycerol Drugs 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
- G01N31/223—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for investigating presence of specific gases or aerosols
- G01N31/224—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for investigating presence of specific gases or aerosols for investigating presence of dangerous gases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/0044—Sulphides, e.g. H2S
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/18—Sulfur containing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/18—Sulfur containing
- Y10T436/182—Organic or sulfhydryl containing [e.g., mercaptan, hydrogen, sulfide, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Combustion & Propulsion (AREA)
- Toxicology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
546475
專利申請案 申請專利範圍更正本 (92年12月w曰) 1. -㈣匕氫或硫化物離子之定量法，該定量法之特徵係 於含有硫化氫或硫化物離子之試樣中，添加金屬離子或 能游離出該金屬離子之化合物，以及與該金屬離子瓦應 而發色之同時，其發色反應亦受到上述硫化氫或硫化物 離子之阻礙或促進之金屬指示劑，藉測定該金屬指示1 之發色★強度而定量之;上述之金屬離子及金屬指示劑係 (A)鋅雔子與吡啶基偶氮化合物之組合或⑻鐵離子與 卩比咬基偶氮化合物或亞梢基胺基g分化合物之組合而構 成。 2·-種高半胱胺酸之定量法，該定量法之特徵係於 胱胺酸之試樣中，添加可與該高半胱胺酸作用而產L ，氫或硫化物離子之成分、金屬離子或能游離出該^ +子之化0物以及與该金屬反應而發色之同時，其發 色反應亦受到上述硫化氫或硫化物離子之阻礙或促進x 之金屬指示劑，藉測定該金屬指示劑之發色強度而定量 之=與高半胱胺酸作用而產生硫化氫或硫化物離子: 成分係包含鄰-乙醯基高絲胺酸裂解酶或l_甲硫胺酸厂 ^ % _，而該金屬離子及金屬指示劑係（a)鋅離子與吡 啶基偶氮化合物之組合或⑻鐵離子與吼。定基偶IU匕合 物或亞硝基胺基酚化合物之組合而構成。 3.如申请專利範圍第2項之高半胱胺酸之定量法，其中兮 311〇〇3 546475 與高半胱胺酸作用 户邻-乙辟其含 產生硫化氫或硫化物離子之成分 心并p乙3皿基向絲胺酿列 炉醇化入物夕《 解S或甲硫胺酸Γ_裂解酶與 子化口物之組合而構成者。 4. 如申請專利範Q ^ . 弟 員之咼半胱胺酸之定量法，苴中* 醇化合物係選自由甲Θ於辟〇 /、T & r…卜兩… 巯基乙醇、二硫代蘇糖 ^ 丙二醇、胱胺所成組群之一者。 5. 一種半胱胺酸之定量法，以量法之特㈣ 酸之試樣中，添加可與該半胱胺酸作用而產生硫化^ 硫化物雄子之成分、金屬離子或能游離出該金屬離子之 化：物以及與該金屬反應而發色之同時，其發色反應 亦丈到上述疏化氫或硫化物離子之阻礙或促進之金屬 指不劑，藉測定該金屬指示劑之發色強度而定量之’該 與半胱胺酸作用而產生硫化氫或硫化物離子之成分係 ^含至少-種選自鄰·乙醯基絲胺酸裂解酶、氰基丙 月女I 〇成酶及半胱胺酸裂解酶所成組群之酵素，而該金 屬離子及金屬指示劑係⑷鋅離子與〇比。定基偶氮化合物 之組合或(Β)鐵離子與咄啶基偶氮化合物或亞硝基胺基 西分化合物之組合而構成。 6. ^申請專利範圍第5項之半胱胺酸之定量法，該與半耽 月女酸作用而產生硫化氫或硫化物離子之成分係包含至 少一種選自鄰_乙醯基絲胺酸裂解酶、々·氰基丙胺酸合 成酶及半胱胺酸裂解酶所成組群之酵素與硫醇化合物 之組合而構成者。 如申請專利範圍第6項之半胱胺酸之定量法，其中硫醇 311003 546475 化合物係選自曱烷硫醇、2-巯基乙醇、二硫代蘇糖醇、 硫代丙三醇、胱胺所成組群之一者。 3]]003 546475
0.025 100 〇 25 50 75 弟3圖（更正圖） L-局半胱胺酸(μΜ) (10¾)¾¾¾
100 200 300 400 500 L-半胱胺酸(μΜ) 第4圖
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34700398A JP4233160B2 (ja) | 1998-12-07 | 1998-12-07 | ホモシステインの定量法 |
| JP08403599A JP4286371B2 (ja) | 1999-03-26 | 1999-03-26 | ホモシステインの定量法 |
| JP14784899A JP4044702B2 (ja) | 1999-05-27 | 1999-05-27 | 硫化水素又は硫化物イオンの定量法及びそれを利用した特定物質の定量法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TW546475B true TW546475B (en) | 2003-08-11 |
Family
ID=27304422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW088120574A TW546475B (en) | 1998-12-07 | 1999-11-25 | A method for quantifying hydrogen sulfide or sulfide ion and a method for quantifying homocysteine or cysteine by using the method |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6969613B1 (zh) |
| EP (1) | EP1143244B1 (zh) |
| KR (1) | KR100639059B1 (zh) |
| CN (1) | CN1178061C (zh) |
| AT (1) | ATE290207T1 (zh) |
| DE (1) | DE69924017T2 (zh) |
| TW (1) | TW546475B (zh) |
| WO (1) | WO2000034771A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI766341B (zh) * | 2019-12-30 | 2022-06-01 | 財團法人工業技術研究院 | 以金屬離子溶液檢測硫化物含量的方法 |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002002802A1 (fr) * | 2000-06-30 | 2002-01-10 | Azwell Inc. | Procede de mesure d'homocysteine totale |
| JP2006149203A (ja) * | 2003-02-19 | 2006-06-15 | Alfresa Pharma Corp | ホモシステインの測定方法 |
| US7244620B2 (en) * | 2003-12-24 | 2007-07-17 | Daiichi Pure Chemicals Co., Ltd. | Method for quantitatively determining a reducing substance and a reagent for quantitative determination |
| US7198890B2 (en) | 2004-01-13 | 2007-04-03 | Daiichi Pure Chemicals Co., Ltd. | Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine |
| US9422160B1 (en) | 2005-10-28 | 2016-08-23 | Element One, Inc. | Method of making a hydrogen sensing pigment |
| CN101101264B (zh) * | 2007-07-26 | 2010-04-14 | 四川大学 | 海水中硫化物的自动分析方法 |
| WO2009070760A1 (en) * | 2007-11-26 | 2009-06-04 | Element One, Inc. | Hydrogen sulfide indicating pigments |
| GB201713017D0 (en) * | 2017-08-14 | 2017-09-27 | Innospec Ltd | Kit and method |
| CN109632677B (zh) * | 2019-02-20 | 2022-01-07 | 深圳锦秀大唐环保科技有限公司 | 用于分光光度法测定的吸收剂及其制备方法 |
| CN113125432A (zh) * | 2019-12-30 | 2021-07-16 | 财团法人工业技术研究院 | 以金属离子溶液检测硫化物含量的方法 |
| CN111518564A (zh) * | 2020-06-24 | 2020-08-11 | 代彦霞 | 一种用于降低土壤中迁移态铅离子的材料及其制备方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4174202A (en) * | 1977-11-28 | 1979-11-13 | The Dow Chemical Company | Kit and method for testing liquids for hydrogen sulfide content |
| JPS5499494A (en) * | 1978-01-23 | 1979-08-06 | Nippon Yuka Kougiyou Kk | Preparation of test paper for measuring sulfuric ion |
| DE3703081A1 (de) | 1987-02-03 | 1988-08-11 | Miles Inc | Verfahren und mittel zum nachweis von thiolen |
| KR100255522B1 (ko) * | 1991-12-25 | 2000-06-01 | 나이또 오사무 | 생체시료중 금속의 측정방법 및 측정용 시약 |
| EP0584568A1 (en) | 1992-08-25 | 1994-03-02 | Eastman Kodak Company | System of detecting and measuring sulfides in a sample |
| JPH06160368A (ja) * | 1992-11-18 | 1994-06-07 | Idemitsu Kosan Co Ltd | 硫黄分の分析方法及びその装置 |
| GB9617683D0 (en) * | 1996-08-23 | 1996-10-02 | Univ Glasgow | Homocysteine desulphurase |
| US5985540A (en) * | 1997-07-24 | 1999-11-16 | Anticancer, Inc. | High specificity homocysteine assays for biological samples |
| US6107100A (en) * | 1998-04-30 | 2000-08-22 | Metaquant Trust | Compounds and methods for determination of thiols |
-
1999
- 1999-11-25 TW TW088120574A patent/TW546475B/zh not_active IP Right Cessation
- 1999-12-07 DE DE69924017T patent/DE69924017T2/de not_active Expired - Lifetime
- 1999-12-07 KR KR1020017007016A patent/KR100639059B1/ko not_active IP Right Cessation
- 1999-12-07 AT AT99973327T patent/ATE290207T1/de not_active IP Right Cessation
- 1999-12-07 EP EP99973327A patent/EP1143244B1/en not_active Expired - Lifetime
- 1999-12-07 WO PCT/JP1999/006847 patent/WO2000034771A1/ja active IP Right Grant
- 1999-12-07 US US09/856,790 patent/US6969613B1/en not_active Expired - Lifetime
- 1999-12-07 CN CNB998141445A patent/CN1178061C/zh not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI766341B (zh) * | 2019-12-30 | 2022-06-01 | 財團法人工業技術研究院 | 以金屬離子溶液檢測硫化物含量的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20010101130A (ko) | 2001-11-14 |
| EP1143244B1 (en) | 2005-03-02 |
| US6969613B1 (en) | 2005-11-29 |
| DE69924017D1 (de) | 2005-04-07 |
| DE69924017T2 (de) | 2005-09-08 |
| EP1143244A1 (en) | 2001-10-10 |
| CN1329720A (zh) | 2002-01-02 |
| ATE290207T1 (de) | 2005-03-15 |
| KR100639059B1 (ko) | 2006-10-27 |
| CN1178061C (zh) | 2004-12-01 |
| EP1143244A4 (en) | 2002-09-18 |
| WO2000034771A1 (fr) | 2000-06-15 |
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