TW201527534A - 細胞培養基 - Google Patents

細胞培養基 Download PDF

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Publication number
TW201527534A
TW201527534A TW103144675A TW103144675A TW201527534A TW 201527534 A TW201527534 A TW 201527534A TW 103144675 A TW103144675 A TW 103144675A TW 103144675 A TW103144675 A TW 103144675A TW 201527534 A TW201527534 A TW 201527534A
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medium
cells
liter
cell
serum replacement
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TW103144675A
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Chinese (zh)
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Adam Elhofy
Allan Weber
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Essential Pharmaceticals Llc
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    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0037Serum-free medium, which may still contain naturally-sourced components
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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TW103144675A 2013-12-20 2014-12-19 細胞培養基 TW201527534A (zh)

Applications Claiming Priority (1)

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US201361918833P 2013-12-20 2013-12-20

Publications (1)

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TW201527534A true TW201527534A (zh) 2015-07-16

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US (2) US9637721B2 (enExample)
EP (1) EP3083978A4 (enExample)
JP (1) JP2017500893A (enExample)
KR (1) KR20160125352A (enExample)
CN (1) CN106062205A (enExample)
AR (1) AR098875A1 (enExample)
AU (1) AU2014364368A1 (enExample)
BR (1) BR112016014488A2 (enExample)
CA (1) CA2934487A1 (enExample)
CL (1) CL2016001553A1 (enExample)
EA (1) EA201691297A1 (enExample)
IL (1) IL246262A0 (enExample)
MX (1) MX2016008251A (enExample)
SG (1) SG11201604994UA (enExample)
TW (1) TW201527534A (enExample)
WO (1) WO2015095651A1 (enExample)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10918102B2 (en) 2014-03-13 2021-02-16 The General Hospital Corporation Devices and methods to improve and assess viability of human livers
MX2017008737A (es) 2014-12-30 2018-01-25 Cell Cure Neurosciences Ltd Poblaciones de celulas rpe y metodos para generar las mismas.
KR102783900B1 (ko) 2015-10-15 2025-03-19 웨이크 포리스트 유니버시티 헬스 사이언시즈 인 비트로 간 구조체의 제조방법 및 그의 용도
EP3362554B1 (en) 2015-10-16 2025-09-03 Wake Forest University Health Sciences Multi-layer airway organoids and methods of making and using the same
US20180369336A1 (en) * 2015-12-03 2018-12-27 Boston Medical Center Corporation B cell-based cancer immunotherapy
CN105567628B (zh) * 2016-01-30 2019-07-12 令世鑫 一种全悬浮培养mdck细胞的低血清培养基
CN105695407B (zh) * 2016-03-15 2019-04-16 佰通生物技术(苏州)有限公司 一种对干细胞具有活化作用的微量元素组合物及其应用
CN108779436A (zh) * 2016-03-18 2018-11-09 中国科学院生物物理研究所 一种细胞培养基和培养基补充物
TW202140776A (zh) 2016-04-26 2021-11-01 美商美國泰福生技股份有限公司 細胞培養基
CN106119186B (zh) * 2016-06-24 2019-10-08 肇庆大华农生物药品有限公司 一种用于全悬浮培养mdck细胞的无血清培养基及其制备方法
CN106497872B (zh) * 2016-12-01 2019-10-11 北京欣博睿丰医疗科技发展有限公司 骨骼肌干细胞无血清培养基及其制备方法和应用
US12087399B2 (en) * 2017-02-01 2024-09-10 Sapphiros Ai Bio Llc Systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of cells
JP7181534B2 (ja) * 2017-03-28 2022-12-01 味の素株式会社 未分化維持培地添加剤
CN107177542A (zh) * 2017-05-25 2017-09-19 句容亿格纳米材料厂 一种促进培养基中谷氨酰胺溶解的优化方法
US12234484B2 (en) * 2017-05-31 2025-02-25 Promocell Gmbh Culture medium for pluripotent stem cells
US12058996B2 (en) * 2017-06-14 2024-08-13 The General Hospital Corporation High subzero cryopreservation
CN107488625B (zh) * 2017-08-31 2018-12-21 广州蕊特生物科技有限公司 一种胎牛血清替代物及其制备方法和应用
CN109395095B (zh) * 2017-09-18 2021-09-24 武汉原生原代生物医药科技有限公司 体内用生物膜及其制备方法和用途
JP7379348B2 (ja) * 2017-10-11 2023-11-14 バスト バイオテクノロジー 線維症の治療のための組成物および方法
JP7117374B2 (ja) * 2017-10-13 2022-08-12 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 灌流培地
WO2019081609A1 (en) * 2017-10-24 2019-05-02 Fundación Instituto De Investigación Sanitaria De Santiago De Compostela (Fidis) NANOSYSTEMS FOR CELL PROLIFERATION
KR102569522B1 (ko) * 2017-11-24 2023-08-24 주식회사 차바이오랩 세포 동결 보존용 조성물 및 이를 이용한 세포 동결 보존 방법
SG11202005795TA (en) * 2017-12-29 2020-07-29 Cell Cure Neurosciences Ltd Retinal pigment epithelium cell compositions
US12167729B2 (en) 2018-05-30 2024-12-17 The General Hospital Corporation Cryopreservation of tissues and organs
CN108815516B (zh) * 2018-06-29 2019-03-15 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv灭活疫苗的方法
CN108853489B (zh) * 2018-06-29 2019-07-02 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv弱毒疫苗的方法
CN109234223B (zh) * 2018-11-21 2021-01-19 南京基蛋生物医药有限公司 低蛋白无血清细胞培养基
KR102271949B1 (ko) * 2019-04-15 2021-07-02 순천향대학교 산학협력단 포스파티딜콜린을 함유하는 선충 배양용 조성물 및 이를 이용한 선충의 배양방법
WO2020218584A1 (ja) * 2019-04-26 2020-10-29 味の素株式会社 細胞培養用組成物
KR102132457B1 (ko) * 2019-05-10 2020-07-09 주식회사 바이오솔루션 세포외 소포를 고농도로 배양하기 위한 세포 배양배지 및 상기 세포 배양배지를 이용한 세포외 소포 고 함유 조건화 배양액의 제조방법
CN109943526B (zh) * 2019-05-23 2019-09-13 广州赛莱拉干细胞科技股份有限公司 一种促间充质干细胞增殖的无血清多肽组合物
WO2021078935A1 (en) * 2019-10-25 2021-04-29 Fundació Centre De Regulació Genòmica Culture media for mycoplasma
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
WO2021108806A1 (en) * 2019-11-29 2021-06-03 Rutgers, The State University Of New Jersey Compositions, kits and methods for storage of blood products and methods of use thereof
AU2021211230A1 (en) * 2020-01-21 2022-09-01 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Utilization of FGF activators in culture media
CN111849860B (zh) * 2020-06-01 2021-12-21 浙江大学 一种利用铁元素调控肠道干细胞分化的方法及应用
WO2021248141A1 (en) * 2020-06-05 2021-12-09 Upside Foods, Inc. Nutrient media for the production of slaughter-free meat
CN111534478A (zh) * 2020-06-24 2020-08-14 肇庆大华农生物药品有限公司 用于培养mdck细胞系的培养基及其制备方法
US20220142150A1 (en) * 2020-11-10 2022-05-12 Axogen Corporation Packaging system for a medical product
CN112514892B (zh) * 2020-12-25 2022-06-14 广州赛莱拉干细胞科技股份有限公司 一种外泌体冻存保护液及其制备方法
CN112501113B (zh) * 2021-02-03 2021-04-27 上海奥浦迈生物科技股份有限公司 一种hek293细胞组合梯度培养基及其用途
CN113061584A (zh) * 2021-05-14 2021-07-02 赵峻岭 一种能促进腺病毒生长的培养基添加物
EP4381045A4 (en) * 2021-08-02 2025-06-04 Montana State University Chemically defined serum replacements for cell culture
US11878036B2 (en) 2022-05-25 2024-01-23 Neuvian LLC Vaginal care compositions and methods of improving vaginal health
EP4563695A1 (en) * 2022-07-28 2025-06-04 Sanyo-Onoda City Public University Corporation Composition for cryopreservation, cryopreservation method, and frozen cell or biological tissue
KR20240177180A (ko) * 2023-06-19 2024-12-27 (주)엑셀세라퓨틱스 T 세포 배양용 배지 조성물
WO2025098952A1 (en) 2023-11-07 2025-05-15 Evonik Operations Gmbh Nutrient preloading of cells
WO2025199245A1 (en) * 2024-03-19 2025-09-25 Immunis, Inc. Method of making and using secretome composition
US12421441B1 (en) 2025-05-19 2025-09-23 King Fahd University Of Petroleum And Minerals Method for microbially enhanced oil recovery

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4721676A (en) * 1982-12-14 1988-01-26 Genencor, Inc. Novel thermophile isolate having thermostable hydrolytic activity
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
DE3827578A1 (de) 1988-08-13 1990-02-15 Siepmann Friedrich W Verfahren und vorrichtung zur bestimmung oxidierbarer wasserinhaltsstoffe einer waessrigen probenfluessigkeit
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US5834312A (en) 1990-01-29 1998-11-10 Hy-Gene, Inc. Process and media for the growth of human epithelia
US5952198A (en) * 1995-05-04 1999-09-14 Bayer Corporation Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition
AU6698196A (en) 1995-08-15 1997-03-12 Universite Libre De Bruxelles Liposomes preparation method and plant
JP4421681B2 (ja) * 1995-10-19 2010-02-24 バイオ‐オリジン エルエルシー 生殖細胞および胚の生存および機能を改善する方法および組成物
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
AU4751697A (en) 1996-10-10 1998-05-05 Douglas Danner Animal cell culture media comprising plant-derived nutrients
US6833271B2 (en) 1996-12-04 2004-12-21 Medi-Cult A/S Serum-free cell culture media
US5985538A (en) 1997-08-01 1999-11-16 Saint Barnabas Medical Center Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt
GB9817805D0 (en) 1998-08-14 1998-10-14 Danisco Use of a composition
DK1226233T3 (da) 1999-08-05 2011-10-03 Abt Holding Co Multipotente voksne stamceller og fremgangsmåder til isolering heraf
US7112576B1 (en) 1999-12-10 2006-09-26 Regents Of The University Of Minnesota Compositions and methods for cryopreservation of peripheral blood lymphocytes
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
WO2003064598A2 (en) 2002-01-25 2003-08-07 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
EP1475434A1 (en) * 2003-05-09 2004-11-10 Oncoscience AG Method for storing tumor cells
WO2005072523A2 (en) 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Biological material and methods and solutions for preservation thereof
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
US20050287666A1 (en) 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
AU2005271723B2 (en) 2004-07-13 2010-12-16 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
US7767453B2 (en) 2004-10-20 2010-08-03 Whitehead Institute For Biomedical Research Cultured hematopoietic stem cells and method for expansion and analysis thereof
WO2006108455A1 (en) 2004-11-02 2006-10-19 Ares Trading S.A. Serum-free cell culture medium for mammalian cells
CA2595638A1 (en) * 2005-01-28 2006-08-03 Canadian Blood Services A method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability
US9101590B2 (en) 2005-07-29 2015-08-11 Yale University Defined culture conditions of human embryonic stem cells
CN101351546B (zh) 2005-12-21 2013-05-29 江苏太瑞生诺生物医药科技有限公司 治疗播散性癌症的方法
EP1994141B1 (en) 2006-02-23 2017-11-15 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
JP5080779B2 (ja) * 2006-10-25 2012-11-21 テルモ株式会社 リポソーム製剤の製造方法
WO2008137641A2 (en) 2007-05-04 2008-11-13 Whitehead Institute For Biomedical Research Ex vivo expansion of human hematopoietic stem cells
EP2188367A4 (en) 2007-08-10 2010-10-27 Whitehead Biomedical Inst HORMON'S TREATIVE TISSUE CULTURE SYSTEM AND USES THEREOF
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
MX2014005723A (es) 2011-11-11 2014-11-25 Essential Pharmaceuticals Llc Kit que comprende factores labiles y de reemplazo de suero.

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