SU1163224A1 - Method of quantitative determining of salsolin hydrochloride - Google Patents

Abstract

THE METHOD OF QUANTITATIVE DETERMINATION OF SALSOLINE HYDROCHLORIDE by treating an analyte sample with a color reagent followed by photometry of the resulting solution, characterized in that, in order to increase the sensitivity and enhancement of the method, K-chloro-1, 4-benzoquinoneimine and pink isopropycin is used as a color-reagent solution. environment universal buffer solution pH 11.0. (Ls

Description

1i

The invention relates to the field of analytical chemistry, namely to methods for the quantitative determination of salsolina hydrochloride - 1-methy-1-6-oxy-7-methoxy-1, 2,3,4-tetrahydroisoquinoline hydrochloride - used in medical practice as an antispasmodic (hypotensive) agent.

A known method for the quantitative determination of salsoline hydrochloride by titration of an analyzed sample is 0.1 n. a solution of perchloric acid in a mixture of oxide mercuric acetate and anhydrous acetic acid;

The disadvantages of this method are low sensitivity (the O, g sample is used), low selectivity (all compounds of a basic nature interfere with the determination) and method complexity, since highly toxic and a are used, aggressive reactions — chloric acid, oxide mercury acetate, anhydrous acetic acid.

The closest to the invention in its technical essence and the achieved result is the method of quantitative determination of salsolina hydrochloride, which consists in processing the sample to be analyzed with 0.5 ml of a 0.3% solution of potassium iodate, 2 MP of phosphoric acid, heating the reaction mixture in a water bath for 1 min followed by photometric measurement of the obtained C2J solution.

The disadvantages of this method are low sensitivity (20 µg / ml) and the complexity of the method, since strict observance of temperature is required (water bath at 55 ° C for 1 min).

The purpose of the invention is to increase the sensitivity and simplify the method

The goal is achieved by the method of quantifying salsolin hydrochloride by treating an analyte sample with a color reagent followed by photometry of the resulting solution, using a solution of M-chloro-1,4-benzo32242 as the color reagent

quinoneimine in isopropanol and treatment is carried out in a universal buffer solution pH 11.0.

Universal buffer solution 5 is described in G31.

Example. Quantitative determination of salsolin hydrochloride in the sub. station.

A portion of the drug in the range of 0 0.015–0.025 g is dissolved in water in a 200 ml volumetric flask and made up to the mark with the same solvent. 3 ml of the solution obtained is placed in a volumetric flask with a capacity of 5-25 ml, 3 ml of 0.1% is added

N-chloro-1,4-benzoquinone imine solution in isopropanol and adjusted to the mark with a universal solution of pH 11.0. In parallel, experiments were carried out with a standard solution of salsolin hydrochloride and control. After 10 min, the optical density of the analyzed solutions was measured against the background of the control using a spectrophotometer at 5,625 nm in cuvettes with a layer thickness of 1 cm.

The calculation of the quantitative content of salsolin hydrochloride is carried out according to the formula

with (. п- - °°°

° 1) 7rzt-

0

where D is the optical density of the analyzed BO solution — the optical density of the standard Co solution is the concentration of the standard

5 of the spectrophotometric solution (0.0012 g in 100 ml) i p - weight, g ", - layer thickness, see. The results of the quantitative determination of salsolin hydrochloride are given in Table. one..

A comparative characteristic of the proposed method and the known one is given in table 2.

five

As is clear from Table 2, the proposed method is 17 times more sensitive than the known one, and is simpler in the implementation technique.

O since it does not require the use of heating and cTpoForo temperature compliance.

Table 1

99, 86

99.35

100.11

100.33

100.05

99.44

100.22

Th,. 20

X 99, 91 5 0.3806

.2 - 0.0038

2

S 10.9313

table 2

U18

four

Water on the ban Missing 55 ° C for Room

1 minute

temperature

Claims (1)

METHOD FOR QUANTITATIVE DETERMINATION OF SALSOLINE HYDROCHLORIDE by processing a sample of an analyte with a color reagent followed by photometric analysis of the resulting solution, characterized in that, in order to increase the sensitivity and simplify the method, a solution of M-chloro-1, 4-benzoquinonimine in isopropanol and processing of lead are used in a universal buffer solution pH 11.0. SU ,,,. 1163224