RU1810799C - Method of quantitative determination of anionic bases - Google Patents

Abstract

Usage: determination of anionic bases used in medical practice as antiseptics (sodium thiosulfate, sodium tetraborate, sodium salicylate), salt laxatives (sodium sulfate), antimicrobial agents (norsulfazole sodium), etc. Summary of the invention: the analyzed sample is processed - a color reagent - 2-hydroxybenzenehydrazone 2,6-dibromo-1,4-benzoquinone in dioxane, followed by photometry of the obtained colored solution. 3 tab.

Description

ate

WITH

The invention relates to analytical chemistry, and in particular to methods for the quantitative determination of anionic bases - salts of weak acids used in medical practice as antiseptics (sodium thiosulfate, sodium tetraborate, sodium salicylate), saline laxatives (sodium sulfate), antimicrobial agents (norsulfazole- sodium) and others.

The purpose of the invention is to increase sensitivity and simplify determination.

Example. Quantification of anionic bases.

The exact weighed portion of the anionic base shown in Table 1 is dissolved in the indicated volume of water in a 50 ml volumetric flask (in the case of sodium sulfate and sodium thiosulfate with a capacity of 250 ml) and the dioxane is adjusted to the mark. In the case of sodium salicylate and sodium tetraborate, 5 ml of the resulting solution is placed in a volumetric flask with a capacity of 25 ml and dioxane

dilution to the mark. 2 ml of the obtained dilution (in the case of norsulfazole sodium - 1 ml) is placed in a 25 ml volumetric flask, the volume of the 0.1% solution of 2-hydroxybenzoylhydrazone 2,6-dibromo-1 indicated in Table 1 is added. 4-benzoquinone and dioxane were diluted to the mark. At the same time, an experiment was conducted with the exact volume of a standard solution of the analyte. (table 1) and control.

The optical density of the colored solutions was measured against a background of control using an Amax spectrophotometer (Table 2) in cuvettes with a layer thickness of 1 cm.

The content of the anionic base is calculated by the formula

g - D K

where D is the optical density of the analyzed solution:

Do is the optical density of the standard solution;

with

about

VI O S

p - hitch, g;

K is the calculated coefficient taking into account the dilution and concentration of the standard solution (Table 1).

The analytical characteristics of the method for determining anionic bases are given in Table 2, and the metrological characteristics of the results of Quantitative determination are shown in Table 3.

Claims (1)

The claims Method for the quantitative determination of anionic bases by processing optimal conditions for the determination of anionic bases Table 2 Analytical characteristics of the method for determining anionic bases an organic solvent and adding an organic color reagent followed by photometric measurement of the resulting solution, characterized in that, with in order to increase the sensitivity and simplify the determination, dioxane is used as an organic solvent, a 0.1% solution of 2-hydroxybenzoylhydrazone 2,6-dibromo-1,4-benzoquinone is used as a color reagent, and the sample is processed at room temperature. fifteen Table 1 Quantification of Anionic Bases Table