SU1397786A1 - Method of quantitative determination of 5-ethyl-5-isoamylbarbiturate of sodium - Google Patents

Abstract

The invention relates to analytical chemistry, in particular, the determination of 5-eth1-5-isoamosil sodium barbiturate (ABN), used as a hypnotic in medicine. In order to increase sensitivity and simplify the process, the sample is treated with another L-toluenesulfonyl-2-chloro-1,4-naphthoquinone imine reagent in dioxane medium. Then the colored solution is photometrized. The sensitivity of the method is 0.019–0.35 mg / ml with a 4 times less time (15 min) analysis without the need for preliminary purification of the solvent. 2 tab. | SL

Description

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The invention relates to analytical chemistry, and specifically to methods for the quantitative determination of 5-ethyl-5-isoamyl barburt sodium (barbamyl), used in medical practice as a hypnotic.

The purpose of the invention is to increase the sensitivity and simplify the method.

PRI m er.r. Quantitative determination of 5-ethyl-5-isoamyl barbiturate sodium in a substance. The exact weight of the analyte within 10,012-0,022 g is dissolved in water in a 50 ml volumetric flask and made up to the mark with water. 2 ml of the obtained JHoro dilution is placed in a 25 ml volumetric flask, 5 ml of a 0.5% dioxane solution of H-toluenesulfonic 1-2-chloro-1,4-naphthoquinone imine are added and, after 10 min, the dioxane is added to the mark . In parallel, experiments were carried out with a standard solution of 5-ethyl-5-iso-sodium sodium barbiturate (0.015 g in 50 mp) and a background solution.

The optical density of the analyzed solutions was measured on the background of the control using a spectrophotometer at 85 them in cuvettes with a layer thickness of 1 cm

The calculation of the amount of barbamyl 5-ethyl-5-isoamosil barbiturite sodium is carried out according to the formula

D-50-25-CC,

GHBY yr

where D is the optical density of the solution being analyzed; Dp is the optical density of the mill

dart solution;

; C is the concentration of a standard spectrophotometric solution (0.00272 g in 100 ml); p - weight, g; 1 — thickness of the layer, see. Results of the quantitative determination of 5-ethyl-5-isoamyl sodium barbitrate are given in Table. one.

Table 1

100.4 100.2

.75, 9507

Continuation of table 1

Comparative characteristics of the known and proposed methods are given in table. 2

Table 2

1-4

0,019-0,035

Time

hold

analysis,

min 60

Pre-cleaning

solvent- fresh distilled pyridine

15

Not required

As can be seen from the table. 2, this method exceeds the known sensitivity by 50–100 times in magnitude. In addition, it is much easier to perform, since it does not require pre-distillation of solvents, multiple extractions, drying the extract, and the analysis time is reduced by 4 times.

Claims (1)

Invention Formula The method of quantitative determination of sodium 5-ethyl-5-isoamyl barbiturate by treating the sample being analyzed with an organic reagent after 31137786. Following photometry of the obtained reagent, an N-toluene solution is used, which differs in sulfonyl-2-chloro-1,4-naphthoquinone imine in that, in order to increase the sensitivity and processing, it is carried out in the environment of dioecidity and simplification of the process. .