SK110797A3 - Osteoprotegerin - Google Patents
Osteoprotegerin Download PDFInfo
- Publication number
- SK110797A3 SK110797A3 SK1107-97A SK110797A SK110797A3 SK 110797 A3 SK110797 A3 SK 110797A3 SK 110797 A SK110797 A SK 110797A SK 110797 A3 SK110797 A3 SK 110797A3
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- opg
- polypeptide
- huopg
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
- A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
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- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/577,788 US6613544B1 (en) | 1995-12-22 | 1995-12-22 | Osteoprotegerin |
| US08/706,945 US6369027B1 (en) | 1995-12-22 | 1996-09-03 | Osteoprotegerin |
| PCT/US1996/020621 WO1997023614A1 (en) | 1995-12-22 | 1996-12-20 | Osteoprotegerin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SK110797A3 true SK110797A3 (en) | 1999-07-12 |
Family
ID=27077338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK1107-97A SK110797A3 (en) | 1995-12-22 | 1996-12-20 | Osteoprotegerin |
Country Status (31)
| Country | Link |
|---|---|
| US (1) | US6369027B1 (ro) |
| EP (4) | EP0870023A1 (ro) |
| JP (1) | JP4657388B2 (ro) |
| KR (1) | KR100463584B1 (ro) |
| CN (1) | CN1318588C (ro) |
| AR (1) | AR004400A1 (ro) |
| AT (1) | ATE409745T1 (ro) |
| AU (1) | AU710587B2 (ro) |
| BG (1) | BG63347B1 (ro) |
| CA (1) | CA2210467C (ro) |
| CZ (1) | CZ292587B6 (ro) |
| DE (1) | DE19654610A1 (ro) |
| DK (1) | DK0784093T3 (ro) |
| EE (1) | EE04643B1 (ro) |
| ES (1) | ES2316152T3 (ro) |
| FR (1) | FR2742767B1 (ro) |
| GB (1) | GB2312899B (ro) |
| HK (1) | HK1001526A1 (ro) |
| HU (1) | HU227482B1 (ro) |
| IL (1) | IL121520A (ro) |
| MX (1) | MX9706193A (ro) |
| NO (1) | NO973699L (ro) |
| NZ (2) | NZ326579A (ro) |
| PL (1) | PL187408B1 (ro) |
| PT (1) | PT784093E (ro) |
| RO (1) | RO121386B1 (ro) |
| SI (1) | SI0784093T1 (ro) |
| SK (1) | SK110797A3 (ro) |
| TR (1) | TR199601036A2 (ro) |
| WO (1) | WO1997023614A1 (ro) |
| YU (1) | YU69196A (ro) |
Families Citing this family (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6919434B1 (en) | 1995-02-20 | 2005-07-19 | Sankyo Co., Ltd. | Monoclonal antibodies that bind OCIF |
| IL117175A (en) * | 1995-02-20 | 2005-11-20 | Sankyo Co | Osteoclastogenesis inhibitory factor protein |
| US7078493B1 (en) | 1995-03-15 | 2006-07-18 | Human Genome Sciences, Inc. | Antibodies to human tumor necrosis factor receptor-like genes |
| US7632922B1 (en) * | 1995-12-22 | 2009-12-15 | Amgen, Inc. | Osteoprotegerin |
| US7005413B1 (en) | 1995-12-22 | 2006-02-28 | Amgen Inc. | Combination therapy for conditions leading to bone loss |
| JPH1057071A (ja) * | 1996-08-19 | 1998-03-03 | Snow Brand Milk Prod Co Ltd | 新規ｄｎａ及びそれを用いた蛋白質の製造方法 |
| ATE406176T1 (de) | 1996-12-06 | 2008-09-15 | Amgen Inc | Il-1-inhibitor in kombinationstherapie zur behandlung il-1-vermittelter krankheiten |
| DE69740107D1 (de) | 1996-12-23 | 2011-03-10 | Immunex Corp | Rezeptor aktivator von nf-kappa b, rezeptor is mitglied der tnf rezeptor superfamilie |
| US6271349B1 (en) | 1996-12-23 | 2001-08-07 | Immunex Corporation | Receptor activator of NF-κB |
| PT911342E (pt) * | 1997-04-15 | 2006-08-31 | Sankyo Co | Nova proteina e processo para a produzir |
| US6316408B1 (en) * | 1997-04-16 | 2001-11-13 | Amgen Inc. | Methods of use for osetoprotegerin binding protein receptors |
| SK288559B6 (sk) | 1997-04-16 | 2018-05-02 | Amgen, Inc. | Nukleová kyselina, polypeptid, expresný vektor a hostiteľská bunka; proteín viažuci osteoprotegerín a spôsob jeho prípravy, protilátka a liečivo na prevenciu alebo liečenie chorôb kostí |
| CA2288351A1 (en) * | 1997-05-01 | 1998-11-05 | Amgen Inc. | Chimeric opg polypeptides |
| WO1999004001A1 (en) * | 1997-07-21 | 1999-01-28 | Zymogenetics, Inc. | Tumor necrosis factor receptor ztnfr-5 |
| JP2001512667A (ja) * | 1997-08-06 | 2001-08-28 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | ヒトオーファンレセプターｎｔｒ−１ |
| US6346388B1 (en) | 1997-08-13 | 2002-02-12 | Smithkline Beecham Corporation | Method of identifying agonist and antagonists for tumor necrosis related receptors TR1 and TR2 |
| WO1999011790A1 (en) * | 1997-09-04 | 1999-03-11 | Zymogenetics, Inc. | Tumor necrosis factor receptor ztnfr-6 |
| DE69839401T2 (de) | 1997-09-18 | 2009-05-07 | Genentech Inc., San Francisco | Dcr3 polypeptid, ein tnfr homolog |
| US6693175B2 (en) * | 1997-09-24 | 2004-02-17 | Sankyo Co., Ltd. | Method for diagnosing bone dysbolism |
| US6087555A (en) * | 1997-10-15 | 2000-07-11 | Amgen Inc. | Mice lacking expression of osteoprotegerin |
| JPH11155420A (ja) * | 1997-12-02 | 1999-06-15 | Snow Brand Milk Prod Co Ltd | トランスジェニック動物 |
| WO1999031128A2 (en) * | 1997-12-16 | 1999-06-24 | Incyte Pharmaceuticals, Inc. | Human tumor necrosis factor-r2-like proteins |
| US6077689A (en) | 1997-12-24 | 2000-06-20 | Amgen Inc. | Enhanced solubility of recombinant proteins |
| AU2589399A (en) * | 1998-02-17 | 1999-08-30 | Incyte Pharmaceuticals, Inc. | Human short-chain tnf-receptor family protein |
| US6150098A (en) * | 1998-02-20 | 2000-11-21 | Amgen Inc. | Methods for identifying novel secreted mammalian polypeptides |
| US6103472A (en) * | 1998-02-20 | 2000-08-15 | Amgen Inc. | Methods and compositions for identifying novel secreted mammalian polypeptides in yeast |
| EP1074564B1 (en) | 1998-04-23 | 2008-12-10 | Ajinomoto Co., Inc. | Substance with antithrombotic activity and method for detecting glycokallidin |
| US6790823B1 (en) * | 1998-04-23 | 2004-09-14 | Amgen Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
| JP2002514418A (ja) | 1998-05-14 | 2002-05-21 | イミュネックス・コーポレーション | 破骨細胞活性の阻害方法 |
| US6210924B1 (en) | 1998-08-11 | 2001-04-03 | Amgen Inc. | Overexpressing cyclin D 1 in a eukaryotic cell line |
| ID28386A (id) | 1998-09-15 | 2001-05-17 | M & E Biotech As | Metode penurunan aktivitas ligan osteoprotegerin |
| US6420339B1 (en) | 1998-10-14 | 2002-07-16 | Amgen Inc. | Site-directed dual pegylation of proteins for improved bioactivity and biocompatibility |
| US6660843B1 (en) | 1998-10-23 | 2003-12-09 | Amgen Inc. | Modified peptides as therapeutic agents |
| US7488590B2 (en) | 1998-10-23 | 2009-02-10 | Amgen Inc. | Modified peptides as therapeutic agents |
| CN1810832B (zh) | 1998-10-23 | 2012-12-12 | 麒麟-安姆根有限公司 | 与MPl受体结合并具有血小板生成活性的模拟二聚体血小板生成素肽 |
| JP3860415B2 (ja) * | 1998-10-28 | 2006-12-20 | 三共株式会社 | 骨代謝異常症治療剤 |
| US6245740B1 (en) | 1998-12-23 | 2001-06-12 | Amgen Inc. | Polyol:oil suspensions for the sustained release of proteins |
| AU3149200A (en) * | 1999-01-18 | 2000-08-01 | Osteometer Biotech As | Genetic predisposition |
| US20030007972A1 (en) * | 1999-02-24 | 2003-01-09 | Edward Tobinick | Cytokine antagonists and other biologics for the treatment of bone metastases |
| US7259253B2 (en) * | 1999-05-14 | 2007-08-21 | Quark Biotech, Inc. | Genes associated with mechanical stress, expression products therefrom, and uses thereof |
| AU6078500A (en) * | 1999-07-09 | 2001-01-30 | Amgen, Inc. | Combination therapy for conditions leading to bone loss |
| AUPQ167599A0 (en) * | 1999-07-19 | 1999-08-12 | St. Vincent's Institute Of Medical Research | Inhibitor of osteoclast precursor formation |
| IL130989A0 (en) | 1999-07-20 | 2001-01-28 | Compugen Ltd | Variants of alternative splicing |
| US6673771B1 (en) * | 1999-07-28 | 2004-01-06 | The Trustees Of The University Of Pennsylvania | Methods of inhibiting osteoclast activity |
| US8106098B2 (en) | 1999-08-09 | 2012-01-31 | The General Hospital Corporation | Protein conjugates with a water-soluble biocompatible, biodegradable polymer |
| AU6946300A (en) * | 1999-08-30 | 2001-03-26 | Mayo Foundation For Medical Education And Research | Use of dna encoding osteoprotegerin to prevent or inhibit metabolic bone disorders |
| AU2005237128B2 (en) * | 1999-09-03 | 2008-09-11 | Amgen Inc. | Compositions and Methods for the Prevention or Treatment of Cancer and Bone Loss Associated with Cancer |
| EP1140136B1 (en) * | 1999-09-03 | 2007-05-16 | Amgen Inc. | Compositions and methods for the prevention or treatment of cancer and bone loss associated with cancer |
| US20030144187A1 (en) * | 1999-09-03 | 2003-07-31 | Colin R. Dunstan | Opg fusion protein compositions and methods |
| US6808902B1 (en) * | 1999-11-12 | 2004-10-26 | Amgen Inc. | Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules |
| AU2262501A (en) * | 1999-12-16 | 2001-06-25 | Amgen, Inc. | Tnfr/opg-like molecules and uses thereof |
| US20030103978A1 (en) | 2000-02-23 | 2003-06-05 | Amgen Inc. | Selective binding agents of osteoprotegerin binding protein |
| PT2077279E (pt) | 2000-06-28 | 2012-09-14 | Amgen Inc | Moléculas do receptor de linfopoietina estromal tímica e suas utilizações |
| MXPA03002443A (es) * | 2000-09-22 | 2004-12-06 | Immunex Corp | Ensayos de clasificacion para agonistas o antagonistas del activador receptor de nf-kb. |
| AU2002231602A1 (en) * | 2001-02-09 | 2002-08-28 | Maxygen Holdings Ltd. | Rank ligand-binding polypeptides |
| AU2002253133B2 (en) | 2001-04-03 | 2008-02-28 | Societe Des Produits Nestle S.A. | Osteoprotegerin in milk |
| JP2005515159A (ja) * | 2001-06-06 | 2005-05-26 | イミュネックス・コーポレーション | 癌を治療するためのｒａｎｋアンタゴニストの使用 |
| EP2087908B1 (en) | 2001-06-26 | 2018-05-30 | Amgen Inc. | Antibodies to opgl |
| CZ20022231A3 (cs) * | 2001-06-29 | 2003-02-12 | Sankyo Company Limited | Komplex skládající se z osteoklastogenezi inhibujícího faktoru a polysacharidu |
| KR100427299B1 (ko) * | 2001-08-10 | 2004-04-14 | 한국생명공학연구원 | 인체 골 재흡수 억제인자（ｈＯＰＧ）를 생산하는 재조합플라스미드 ｐＧＨＯＰＧ（ＫＣＴＣ １０１９ＢＰ） |
| US20030049694A1 (en) * | 2001-09-10 | 2003-03-13 | Chung-Hsiun Wu | Production of fusion proteins and use for identifying binding molecules |
| US6800462B2 (en) * | 2001-09-10 | 2004-10-05 | Abgenomics Corporation | Production of recombinant proteins in vivo and use for generating antibodies |
| US7521053B2 (en) | 2001-10-11 | 2009-04-21 | Amgen Inc. | Angiopoietin-2 specific binding agents |
| US7138370B2 (en) | 2001-10-11 | 2006-11-21 | Amgen Inc. | Specific binding agents of human angiopoietin-2 |
| US7205275B2 (en) | 2001-10-11 | 2007-04-17 | Amgen Inc. | Methods of treatment using specific binding agents of human angiopoietin-2 |
| JP4336467B2 (ja) * | 2001-10-15 | 2009-09-30 | 株式会社林原生物化学研究所 | 破骨細胞形成抑制因子の産生を調節し得る物質のスクリーニング方法 |
| US20030191056A1 (en) | 2002-04-04 | 2003-10-09 | Kenneth Walker | Use of transthyretin peptide/protein fusions to increase the serum half-life of pharmacologically active peptides/proteins |
| WO2003086289A2 (en) * | 2002-04-05 | 2003-10-23 | Amgen Inc. | Human anti-opgl neutralizing antibodies as selective opgl pathway inhibitors |
| US20080249068A1 (en) * | 2002-09-05 | 2008-10-09 | Deluca Hector F | Method of Extending the Dose Range of Vitamin D Compounds |
| US7259143B2 (en) * | 2002-09-05 | 2007-08-21 | Wisconsin Alumni Research Foundation | Method of extending the dose range of vitamin D compounds |
| WO2004052233A2 (en) | 2002-12-10 | 2004-06-24 | Schering-Plough Ltd. | Canine rankl and methods for preparing and using the same |
| TWI293882B (en) | 2003-03-24 | 2008-03-01 | Sankyo Co | Polymeric modifiers and pharmaceutical compositions |
| WO2006010057A2 (en) | 2004-07-08 | 2006-01-26 | Amgen Inc. | Therapeutic peptides |
| ATE544781T1 (de) * | 2004-12-13 | 2012-02-15 | Cephalon Australia Vic Pty Ltd | Osteoprotegerinvarianten-proteine |
| US8008453B2 (en) | 2005-08-12 | 2011-08-30 | Amgen Inc. | Modified Fc molecules |
| JO3324B1 (ar) | 2006-04-21 | 2019-03-13 | Amgen Inc | مركبات علاجية مجففة بالتبريد تتعلق بالعصارة الهضمية |
| CA2687141C (en) | 2007-05-22 | 2014-04-01 | Amgen Inc. | Compositions and methods for producing bioactive fusion proteins |
| EP2464377B1 (en) | 2009-08-14 | 2016-07-27 | The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services | Use of il-15 to increase thymic output and to treat lymphopenia |
| DK3241558T3 (da) | 2010-09-28 | 2021-04-26 | Aegerion Pharmaceuticals Inc | Højopløselige leptiner |
| KR102323287B1 (ko) | 2011-10-05 | 2021-11-05 | 원드 매터리얼 인코포레이티드 | 리튬 이온 배터리용 실리콘 나노구조체 활물질, 및 그에 관련된 공정, 조성물, 구성요소 및 디바이스 |
| CA2868759A1 (en) * | 2012-03-31 | 2013-10-03 | R-PHARM, CJSC (Closed Joint Stock Company) | Osteoprotegerin derived composition and use thereof |
| US11680101B2 (en) | 2017-01-27 | 2023-06-20 | Kymab Limited | Anti-OPG antibodies |
| JP6550413B2 (ja) * | 2017-02-24 | 2019-07-24 | アール−ファーム・インターナショナル・リミテッド・ライアビリティ・カンパニーＲ−Ｐｈａｒｍ Ｉｎｔｅｒｎａｔｉｏｎａｌ， Ｌｌｃ | オステオプロテゲリン由来の組成物およびその使用 |
| CN111004318B (zh) * | 2019-12-30 | 2022-03-04 | 北京博康健基因科技有限公司 | rhPTH(1-34)蛋白原液的纯化方法 |
| TW202203964A (zh) * | 2020-04-17 | 2022-02-01 | 小利蘭史丹佛大學董事會 | 用於生物醫藥調配物之聚合物賦形劑 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4179337A (en) | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
| US6936694B1 (en) | 1982-05-06 | 2005-08-30 | Intermune, Inc. | Manufacture and expression of large structural genes |
| US4710473A (en) | 1983-08-10 | 1987-12-01 | Amgen, Inc. | DNA plasmids |
| FR2640537B1 (fr) | 1988-12-21 | 1992-02-21 | Levy Guy | Installation et procede utilisant l'effet laser, pour la coupe ou la vaporisation de materiaux et tissus divers |
| CA2006596C (en) | 1988-12-22 | 2000-09-05 | Rika Ishikawa | Chemically-modified g-csf |
| AU648505B2 (en) | 1989-05-19 | 1994-04-28 | Amgen, Inc. | Metalloproteinase inhibitor |
| US5395760A (en) * | 1989-09-05 | 1995-03-07 | Immunex Corporation | DNA encoding tumor necrosis factor-α and -β receptors |
| US5118667A (en) * | 1991-05-03 | 1992-06-02 | Celtrix Pharmaceuticals, Inc. | Bone growth factors and inhibitors of bone resorption for promoting bone formation |
| CA2068389A1 (en) * | 1991-05-13 | 1992-11-14 | Masahiko Sato | Method for inhibiting bone resorption |
| US5447851B1 (en) | 1992-04-02 | 1999-07-06 | Univ Texas System Board Of | Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells |
| JP3284481B2 (ja) | 1993-08-20 | 2002-05-20 | 本田技研工業株式会社 | 車両用油圧作動式変速機の油圧制御回路 |
| US6268212B1 (en) | 1993-10-18 | 2001-07-31 | Amgen Inc. | Tissue specific transgene expression |
| JP3433495B2 (ja) | 1993-12-30 | 2003-08-04 | カシオ計算機株式会社 | 表示制御装置および表示制御方法 |
| US6309853B1 (en) * | 1994-08-17 | 2001-10-30 | The Rockfeller University | Modulators of body weight, corresponding nucleic acids and proteins, and diagnostic and therapeutic uses thereof |
| IL117175A (en) | 1995-02-20 | 2005-11-20 | Sankyo Co | Osteoclastogenesis inhibitory factor protein |
| WO1996028546A1 (en) | 1995-03-15 | 1996-09-19 | Human Genome Sciences, Inc. | Human tumor necrosis factor receptor |
| US8615703B2 (en) | 2010-06-04 | 2013-12-24 | Micron Technology, Inc. | Advanced bitwise operations and apparatus in a multi-level system with nonvolatile memory |
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