SG11201808573YA - Nucleic acid stabilization reagent, kits, and methods of use thereof - Google Patents
Nucleic acid stabilization reagent, kits, and methods of use thereofInfo
- Publication number
- SG11201808573YA SG11201808573YA SG11201808573YA SG11201808573YA SG11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA
- Authority
- SG
- Singapore
- Prior art keywords
- co7d
- international
- kits
- suite
- methods
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000006641 stabilisation Effects 0.000 title abstract 3
- 238000011105 stabilization Methods 0.000 title abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- KHOITXIGCFIULA-UHFFFAOYSA-N Alophen Chemical compound C1=CC(OC(=O)C)=CC=C1C(C=1N=CC=CC=1)C1=CC=C(OC(C)=O)C=C1 KHOITXIGCFIULA-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0635—B lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662316514P | 2016-03-31 | 2016-03-31 | |
| PCT/US2017/025065 WO2017173105A1 (en) | 2016-03-31 | 2017-03-30 | Nucleic acid stabilization reagent, kits, and methods of use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201808573YA true SG11201808573YA (en) | 2018-10-30 |
Family
ID=59966482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201808573YA SG11201808573YA (en) | 2016-03-31 | 2017-03-30 | Nucleic acid stabilization reagent, kits, and methods of use thereof |
Country Status (10)
| Country | Link |
|---|---|
| EP (2) | EP4043475B1 (zh) |
| JP (2) | JP7019590B2 (zh) |
| KR (2) | KR102451535B1 (zh) |
| CN (2) | CN115896099A (zh) |
| AU (1) | AU2017239666B2 (zh) |
| CA (1) | CA3020728A1 (zh) |
| DK (1) | DK3436469T3 (zh) |
| IL (1) | IL262019A (zh) |
| SG (1) | SG11201808573YA (zh) |
| WO (1) | WO2017173105A1 (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2017375631B2 (en) | 2016-12-12 | 2023-06-15 | xCella Biosciences, Inc. | Methods and systems for screening using microcapillary arrays |
| WO2019133874A1 (en) * | 2017-12-31 | 2019-07-04 | Berkeley Lights, Inc. | General functional assay |
| EP3962652A4 (en) * | 2019-04-30 | 2023-01-18 | Berkeley Lights, Inc. | METHODS FOR ENCAPSULATION AND TESTING OF CELLS |
| JP2022543668A (ja) * | 2019-08-09 | 2022-10-13 | ナットクラッカー セラピューティクス， インコーポレイテッド | マイクロ流体装置およびその使用方法 |
| GB2604481A (en) | 2019-10-10 | 2022-09-07 | 1859 Inc | Methods and systems for microfluidic screening |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6204375B1 (en) * | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
| GB9828709D0 (en) | 1998-12-24 | 1999-02-17 | Novartis Ag | Assay |
| US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
| US6942776B2 (en) | 1999-05-18 | 2005-09-13 | Silicon Biosystems S.R.L. | Method and apparatus for the manipulation of particles by means of dielectrophoresis |
| EP1229823A1 (en) | 1999-11-17 | 2002-08-14 | Glaxo Group Limited | Infrared thermography |
| DK1326963T3 (da) * | 2000-10-17 | 2008-08-25 | Zeptometrix Corp | Mikroorganismer, som er gjort ikke-patogene |
| WO2002056030A2 (en) * | 2000-11-08 | 2002-07-18 | Becton Dickinson Co | Method and device for collecting and stabilizing a biological sample |
| US20020197637A1 (en) * | 2001-06-02 | 2002-12-26 | Willson Richard C. | Process and compositions for protection of nucleic acids |
| US6958132B2 (en) | 2002-05-31 | 2005-10-25 | The Regents Of The University Of California | Systems and methods for optical actuation of microfluidics based on opto-electrowetting |
| US7977049B2 (en) | 2002-08-09 | 2011-07-12 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
| WO2005005044A1 (en) * | 2003-07-10 | 2005-01-20 | Universite Libre De Bruxelles | Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed |
| US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| EP1735428A4 (en) | 2004-04-12 | 2010-11-10 | Univ California | OPTOELECTRONIC TWEEZERS FOR MANIPULATING MICROPARTICLES AND CELLS |
| WO2008091848A2 (en) | 2007-01-22 | 2008-07-31 | Advanced Liquid Logic, Inc. | Surface assisted fluid loading and droplet dispensing |
| WO2008119066A1 (en) | 2007-03-28 | 2008-10-02 | The Regents Of The University Of California | Single-sided lateral-field and phototransistor-based optoelectronic tweezers |
| CN102124126A (zh) | 2007-10-26 | 2011-07-13 | 生命技术公司 | 使用非随机引物的cdna合成 |
| EP2265730B1 (en) | 2008-03-12 | 2018-06-06 | The Rockefeller University | Methods and compositions for translational profiling and molecular phenotyping |
| FR2952072B1 (fr) | 2009-11-05 | 2013-09-27 | Pf Medicament | Procede de production de triptolide |
| US9533306B2 (en) | 2010-08-02 | 2017-01-03 | The Regents Of The University Of California | Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns |
| WO2012024658A2 (en) * | 2010-08-20 | 2012-02-23 | IntegenX, Inc. | Integrated analysis system |
| US9051563B2 (en) * | 2011-01-14 | 2015-06-09 | Zymo Research Corporation | Nucleic acid purification |
| US9227200B2 (en) | 2011-06-03 | 2016-01-05 | The Regents Of The University Of California | Microfluidic devices with flexible optically transparent electrodes |
| US9857333B2 (en) | 2012-10-31 | 2018-01-02 | Berkeley Lights, Inc. | Pens for biological micro-objects |
| US9403172B2 (en) | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
| EP2954050A2 (en) | 2013-02-08 | 2015-12-16 | European Molecular Biology Laboratory | Improved baculoviral expression system and methods of producing the same |
| JP6407248B2 (ja) * | 2013-03-18 | 2018-10-17 | キアゲン ゲーエムベーハー | 細胞外核酸の安定化および単離 |
| EP3007556B1 (en) | 2013-06-13 | 2020-05-20 | Biomatrica, INC. | Cell stabilization |
| EP3060645B1 (en) | 2013-10-22 | 2019-01-02 | Berkeley Lights, Inc. | Microfluidic devices having isolation pens and methods of testing biological micro-objects with same |
| US9889445B2 (en) | 2013-10-22 | 2018-02-13 | Berkeley Lights, Inc. | Micro-fluidic devices for assaying biological activity |
| US20150166326A1 (en) * | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
| EA201692018A1 (ru) | 2014-04-08 | 2017-04-28 | Ньюроувив Фармасьютикал Аб | Пролекарства янтарной кислоты для повышения производства atp |
| US20150306598A1 (en) | 2014-04-25 | 2015-10-29 | Berkeley Lights, Inc. | DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus |
| US20150306599A1 (en) | 2014-04-25 | 2015-10-29 | Berkeley Lights, Inc. | Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus |
| KR102232094B1 (ko) | 2014-04-25 | 2021-03-25 | 버클리 라잇츠, 인크. | 동일한 미세유체 장치에의 dep 처리 디바이스들 및 제어가능 전기습윤 디바이스들의 제공 |
| WO2015188171A1 (en) * | 2014-06-06 | 2015-12-10 | Berkeley Lights, Inc. | Isolating microfluidic structures and trapping bubbles |
| CN106662512A (zh) * | 2014-06-10 | 2017-05-10 | 生物马特里卡公司 | 在环境温度下稳定血液样品中的非变性多肽、核酸和外来体 |
| CN105636937B (zh) * | 2015-01-27 | 2018-07-17 | 深圳华大生命科学研究院 | 用于保存生物样品的稳定剂 |
| CN105158455B (zh) * | 2015-09-17 | 2018-10-16 | 深圳市钠科生物有限公司 | 一种提高样品检测精度和准确性的生物样品采集方法、装置、系统、生物样品稳定试剂及应用 |
-
2017
- 2017-03-30 CA CA3020728A patent/CA3020728A1/en active Pending
- 2017-03-30 WO PCT/US2017/025065 patent/WO2017173105A1/en active Application Filing
- 2017-03-30 EP EP21217724.0A patent/EP4043475B1/en active Active
- 2017-03-30 DK DK17776666.4T patent/DK3436469T3/da active
- 2017-03-30 KR KR1020187031380A patent/KR102451535B1/ko active IP Right Grant
- 2017-03-30 KR KR1020227034059A patent/KR20220139426A/ko not_active Application Discontinuation
- 2017-03-30 CN CN202310097875.7A patent/CN115896099A/zh active Pending
- 2017-03-30 CN CN201780033808.1A patent/CN109311930B/zh active Active
- 2017-03-30 AU AU2017239666A patent/AU2017239666B2/en active Active
- 2017-03-30 EP EP17776666.4A patent/EP3436469B1/en active Active
- 2017-03-30 SG SG11201808573YA patent/SG11201808573YA/en unknown
- 2017-03-30 JP JP2018550743A patent/JP7019590B2/ja active Active
-
2018
- 2018-09-27 IL IL262019A patent/IL262019A/en unknown
-
2022
- 2022-02-02 JP JP2022014717A patent/JP2022064981A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2019513363A (ja) | 2019-05-30 |
| DK3436469T3 (da) | 2022-03-21 |
| EP4043475B1 (en) | 2023-09-20 |
| KR20220139426A (ko) | 2022-10-14 |
| KR20180131583A (ko) | 2018-12-10 |
| EP3436469A1 (en) | 2019-02-06 |
| IL262019A (en) | 2018-10-31 |
| KR102451535B1 (ko) | 2022-10-05 |
| EP4043475A1 (en) | 2022-08-17 |
| JP7019590B2 (ja) | 2022-02-15 |
| AU2017239666A1 (en) | 2018-11-08 |
| CN109311930A (zh) | 2019-02-05 |
| WO2017173105A1 (en) | 2017-10-05 |
| AU2017239666B2 (en) | 2021-07-29 |
| JP2022064981A (ja) | 2022-04-26 |
| CN115896099A (zh) | 2023-04-04 |
| EP3436469B1 (en) | 2022-01-05 |
| US20190085375A1 (en) | 2019-03-21 |
| EP3436469A4 (en) | 2019-12-11 |
| CN109311930B (zh) | 2023-02-24 |
| CA3020728A1 (en) | 2017-10-05 |
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