SG11201804275TA - Microfluidic devices and kits and methods for use thereof - Google Patents

Microfluidic devices and kits and methods for use thereof

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Publication number
SG11201804275TA
SG11201804275TA SG11201804275TA SG11201804275TA SG11201804275TA SG 11201804275T A SG11201804275T A SG 11201804275TA SG 11201804275T A SG11201804275T A SG 11201804275TA SG 11201804275T A SG11201804275T A SG 11201804275TA SG 11201804275T A SG11201804275T A SG 11201804275TA
Authority
SG
Singapore
Prior art keywords
international
emeryville
suite
street
horton
Prior art date
Application number
SG11201804275TA
Inventor
Kristin Beaumont
Peter Beemiller
Volker Kurz
Gregory Lavieu
Xiaohua Wang
Aathavan Karunakaran
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201804275TA publication Critical patent/SG11201804275TA/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -' Organization International Bureau irestYlid (43) International Publication Date .... ..1. .:*1 15 June 2017(15.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/100347 Publication Al Number (51) International Patent Classification: (81) Designated States (unless G01N 33/543 (2006.01) BOIL 3/00 (2006.01) kind of national protection AO, AT, AU, AZ, BA, (21) International Application Number: BZ, CA, CH, CL, CN, PCT/US2016/065426 DO, DZ, EC, EE, EG, (22) International Filing Date: HN, HR, HU, ID, IL, 7 December 2016 (07.12.2016) KW, KZ, LA, LC, LK, MG, MK, MN, MW, (25) Filing Language: English OM, PA, PE, PG, PH, (26) Publication Language: English SC, SD, SE, SG, SK, TN, TR, TT, TZ, UA, (30) Priority Data: ZW. 62/264,665 8 December 2015 (08.12.2015) US 62/333,821 9 May 2016 (09.05.2016) US (84) Designated States (unless 62/418,625 7 November 2016 (07.11.2016) US kind of regional protection GM, KE, LR, LS, MW, IN, MX, CO, ES, PL, SL, otherwise indicated, for every available): AE, AG, AL, AM, BB, BG, BH, BN, BR, BW, BY, CR, CU, CZ, DE, DJ, DK, DM, FI, GB, GD, GE, GH, GM, GT, IR, IS, JP, KE, KG, KN, KP, KR, LR, LS, LU, LY, MA, MD, ME, MY, MZ, NA, NG, NI, NO, NZ, PT, QA, RO, RS, RU, RW, SA, SM, ST, SV, SY, TH, TJ, TM, UG, US, UZ, VC, VN, ZA, ZM, otherwise indicated, for every available): ARIPO (BW, GH, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 TZ, UG, ZM, ZW), Eurasian Horton Street, Suite 320, Emeryville, CA 94608 (US). TJ, TM), European (AL, (72) Inventors: BEAUMONT, Kristin, G.; 5858 Horton DK, EE, ES, FI, FR, L V, MC, MK, MT, NL, Street, Suite 320, Emeryville, CA 94608 (US). BEEM- SM, TR), OAPI (BF, ILLER, Peter, J.; 5858 Horton Street, Suite 320, Emeryville, CA 94608 (US). KURZ, Volker, L., S.; 5858 GW, KM, ML, MR, NE, GB, 13J, (AM, AZ, BY, KG, KZ, RU, AT, BE, BG, CH, CY, CZ, DE, GR, HR, HU, IE, IS, IT, LT, LU, NO, PL, PT, RO, RS, SE, SI, SK, CF, CG, CI, CM, GA, GN, GQ, SN, TD, TG). Horton Street, Suite 320, Emeryville, CA 94608 (US). Published: LAVIEU, Gregory, G.; 5858 Horton Street, Suite 320, with international search Emeryville, CA 94608 WANG, Xiaohua; 5858 Hor- (US). 21(3)) report (Art ton Street, Suite 320, Emeryville, CA 94608 (US). Agents: KUAN, Roger, C. K&L Gates LLP, 630 (74) et al.; Hansen Way, Palo Alto, CA 94304 (US). (54) Title: MICROFLUIDIC DEVICES AND KITS AND METHODS FOR USE THEREOF 4 240- Th 17 40 a ler Ca it all 58 0 1 , 16 r or 102 -45, 8 10 a -4 „„„„.„„„„„„„„„„„„ D 6 I 2 212 164 zo 168 - - — 2 dl 1 e gr y T I Ot P 141 2VB 4 4 PAodule .Module .Module Module M.-,dules Ai. 1421_104 L2u8 __ ---D FIG. 1B -- 52 501 5---- r---- 2a --542-, Or 202Th 120 6 0 222 28 208 211e 109 2 r104 2 102 1•%. .7 124 --4. 1 I 4 1 14e: 41 R fla 1 Il .4t IN it M 180 _ 17r2 ,, T \ • 9 &._ 2-r _ 4., , ' 1 4 .1/4.,,,,:::_ 10 Pover 20)C-9,_, f5-- : --/ ; I. 54 04 18 24-'ena YY Y 2' 122 a -a$, 0 MG go! ' . 9'k Z J . il k, • 4.,g \Wit214 ta'r ‘1207i '.'ar N 214 q nak-\214 214 0 z14a--' 0 1-1 FIG. 1A IN 1-1 (57) : In situ-generated microfluidic capture structures incorporating a solidified N and use, compositions and kits therefor are described. Microfluidic capture structures may fonned within the microfluidic environment, providing flexibility in assaying micro-objects FIG. 1C polymer network, methods of preparation be advantageously used for assays per - such as biological cells. Assay reagents 0 and analytes may be incorporated within the microfluidic capture structures.
SG11201804275TA 2015-12-08 2016-12-07 Microfluidic devices and kits and methods for use thereof SG11201804275TA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562264665P 2015-12-08 2015-12-08
US201662333821P 2016-05-09 2016-05-09
US201662418625P 2016-11-07 2016-11-07
PCT/US2016/065426 WO2017100347A1 (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and methods for use thereof

Publications (1)

Publication Number Publication Date
SG11201804275TA true SG11201804275TA (en) 2018-06-28

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SG11201804275TA SG11201804275TA (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and methods for use thereof

Country Status (12)

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US (3) US10705082B2 (en)
EP (2) EP3387438B1 (en)
JP (2) JP7083752B2 (en)
KR (2) KR20220153685A (en)
CN (2) CN114878807A (en)
AU (1) AU2016365724B2 (en)
CA (1) CA3005077C (en)
DK (1) DK3387438T3 (en)
IL (2) IL297205A (en)
SG (1) SG11201804275TA (en)
TW (2) TWI758265B (en)
WO (1) WO2017100347A1 (en)

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