SG11201808573YA - Nucleic acid stabilization reagent, kits, and methods of use thereof - Google Patents

Nucleic acid stabilization reagent, kits, and methods of use thereof

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Publication number
SG11201808573YA
SG11201808573YA SG11201808573YA SG11201808573YA SG11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA SG 11201808573Y A SG11201808573Y A SG 11201808573YA
Authority
SG
Singapore
Prior art keywords
co7d
international
kits
suite
methods
Prior art date
Application number
SG11201808573YA
Inventor
Jason M Mcewen
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201808573YA publication Critical patent/SG11201808573YA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1101111 II HEIN HO 11111 01E010 Hill 010111110111110 HOED International Bureau .. .... ..Yejd (10) International Publication Number (43) International Publication Date ..... ...r .....1 WO 2017/173105 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) International Patent Classification: (30) Priority Data: C07H 21/02 (2006.01) C07D 221/00 (2006.01) 62/316,514 31 March 2016 (31.03.2016) US C07H 21/04 (2006.01) CO7D 303/04 (2006.01) CO7D 209/00 (2006.01) CO7D 307/77 (2006.01) (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 Hoon Street, Suite 320, Emeryville, California 94608 CO7D 211/04 (2006.01) CO7D 321/00 (2006.01) rt CO7D 217/02 (2006.01) (US). (72) Inventor: MCEWEN, Jason M.; 5858 Horton Street, (21) International Application Number: PCT/US2017/025065 Suite 320, Emeryville, California 94608 (US). (22) International Filing Date: (74) Agents: MCNEILL, Rebecca M. et al.; McNeill Baur 30 March 2017 (30.03.2017) PLLC, 125 Cambridge Park Drive, Suite 301, Cambridge, Massachusetts 02140 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, [Continued on next page] (54) Title: NUCLEIC , r Co oeocs4 ACID STABILIZATION REAGENT, KITS, AND METHODS OF USE THEREOF (57) : Reagents for stabilizing the nucleic acids of a biological cell, PA: p compositions, kits and methods of use thereof are described. The stabilization = the nucleic acids within the biological for reagents may prepare cell storage and 4 6 ,189 preserve the representative population of the nucleic acids for later isolation and =Module P odue Other Modulus analysis. = 2 71A 100 - .r.O . 1 9 = = = * 1 = \ \_ . 114 1 110. r. xrar i s4 aca FIG. 1A = 102-, ' '-'''° 10 = ir - 20t pox Liu FIG. 1B = 200-, r f Il 214- kr) 214 © 11 en , ,js-214 's- 214 -214 Ir...' 212 14. j 1,- 11 IN IN FIG. IC 1-1 0 ei O WO 2017/173105 Al MIDEDIMOMOIDEIRMEMOMMEHOM010 IIMMOVOIMIE BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, RU, KZ, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, KG, LU, MZ, TJ, TM), European (AL, AT, BE, BG, CH, CY, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, CZ, IT, RS, SE, DE, LT, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, PT, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, SL, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). UG, GA, US, UZ, VC, VN, ZA, ZM, ZW. Published: (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, with international search report (Art. 21(3))
SG11201808573YA 2016-03-31 2017-03-30 Nucleic acid stabilization reagent, kits, and methods of use thereof SG11201808573YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662316514P 2016-03-31 2016-03-31
PCT/US2017/025065 WO2017173105A1 (en) 2016-03-31 2017-03-30 Nucleic acid stabilization reagent, kits, and methods of use thereof

Publications (1)

Publication Number Publication Date
SG11201808573YA true SG11201808573YA (en) 2018-10-30

Family

ID=59966482

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201808573YA SG11201808573YA (en) 2016-03-31 2017-03-30 Nucleic acid stabilization reagent, kits, and methods of use thereof

Country Status (11)

Country Link
US (1) US20190085375A1 (en)
EP (2) EP3436469B1 (en)
JP (2) JP7019590B2 (en)
KR (2) KR20220139426A (en)
CN (2) CN115896099A (en)
AU (1) AU2017239666B2 (en)
CA (1) CA3020728A1 (en)
DK (1) DK3436469T3 (en)
IL (1) IL262019A (en)
SG (1) SG11201808573YA (en)
WO (1) WO2017173105A1 (en)

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WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
KR20210018219A (en) 2018-06-06 2021-02-17 지머젠 인코포레이티드 Modification of genes involved in signaling to control fungal morphology during fermentation and production
CN114126762B (en) * 2019-04-30 2023-01-03 伯克利之光生命科技公司 Methods for encapsulating and assaying cells
CN214973877U (en) 2019-08-09 2021-12-03 胡桃钳医疗公司 Microfluidic device and microfluidic path device for forming therapeutic polynucleotides
EP4041310A1 (en) 2019-10-10 2022-08-17 1859, Inc. Methods and systems for microfluidic screening
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

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Also Published As

Publication number Publication date
CN109311930A (en) 2019-02-05
US20190085375A1 (en) 2019-03-21
JP2019513363A (en) 2019-05-30
KR20180131583A (en) 2018-12-10
EP4043475A1 (en) 2022-08-17
DK3436469T3 (en) 2022-03-21
EP3436469A1 (en) 2019-02-06
KR102451535B1 (en) 2022-10-05
AU2017239666B2 (en) 2021-07-29
EP4043475B1 (en) 2023-09-20
AU2017239666A1 (en) 2018-11-08
JP2022064981A (en) 2022-04-26
CN115896099A (en) 2023-04-04
IL262019A (en) 2018-10-31
KR20220139426A (en) 2022-10-14
WO2017173105A1 (en) 2017-10-05
CN109311930B (en) 2023-02-24
EP3436469A4 (en) 2019-12-11
EP3436469B1 (en) 2022-01-05
JP7019590B2 (en) 2022-02-15
CA3020728A1 (en) 2017-10-05

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