SG109552A1 - Thermostable taq polymerase fragment - Google Patents

Thermostable taq polymerase fragment

Info

Publication number
SG109552A1
SG109552A1 SG200404410A SG200404410A SG109552A1 SG 109552 A1 SG109552 A1 SG 109552A1 SG 200404410 A SG200404410 A SG 200404410A SG 200404410 A SG200404410 A SG 200404410A SG 109552 A1 SG109552 A1 SG 109552A1
Authority
SG
Singapore
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
SG200404410A
Other languages
English (en)
Inventor
Waltraud Ankenbauer Dr
Thomas Meier Dr
Annette Deufel Dr
Dieter Heindl Dr
Gisela Betzl Dr
Rainer Schmuck Dr
Bernd Schneidinger Dr
Strey Jessica
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG109552A1 publication Critical patent/SG109552A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SG200404410A 2003-08-12 2004-08-11 Thermostable taq polymerase fragment SG109552A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
SG109552A1 true SG109552A1 (en) 2005-03-30

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
SG200404410A SG109552A1 (en) 2003-08-12 2004-08-11 Thermostable taq polymerase fragment

Country Status (15)

Country Link
US (1) US7972830B2 (fr)
EP (1) EP1507002B1 (fr)
JP (1) JP3929061B2 (fr)
KR (1) KR100626578B1 (fr)
CN (1) CN1590543B (fr)
AT (1) ATE348881T1 (fr)
AU (1) AU2004203649B2 (fr)
BR (1) BRPI0403221A (fr)
CA (1) CA2474734C (fr)
DE (1) DE602004003755T2 (fr)
DK (1) DK1507002T3 (fr)
ES (1) ES2278249T3 (fr)
HK (1) HK1074458A1 (fr)
MX (1) MXPA04007721A (fr)
SG (1) SG109552A1 (fr)

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EP1841888A4 (fr) * 2005-01-04 2009-05-27 Stratagene California Réaction en chaine de la polymérase à amorçage à chaud à l'aide d'un bloqueur thermolabile
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (fr) 2008-04-28 2009-11-04 Biotype AG Substances et procédés pour un dosage de profilage à base d'ADN
CA2722541C (fr) 2008-04-30 2017-01-10 Integrated Dna Technologies, Inc. Dosages a base de rnase-h utilisant des monomeres d'arn modifies
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
WO2010062777A2 (fr) * 2008-11-03 2010-06-03 Kapabiosystems Adn polymérases de type a modifiées
WO2011061625A2 (fr) * 2009-11-19 2011-05-26 Solis Biodyne Compositions pour augmenter la stabilité et l'activité d'un polypeptide, et procédés apparentés
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012135053A2 (fr) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Essais à base d'arnase h faisant appel à des monomères d'arn modifiés
WO2012146260A1 (fr) 2011-04-23 2012-11-01 Biolytix Ag Préparation et utilisation de protéines en biologie moléculaire
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
CN107208074B (zh) * 2014-12-16 2021-06-15 生命技术公司 聚合酶组合物和制造与使用其的方法
CN107636164B (zh) 2015-05-29 2022-02-18 豪夫迈·罗氏有限公司 用柠康酸酐灭活微生物的方法
JP6963238B2 (ja) * 2015-11-27 2021-11-05 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
US20200181666A1 (en) * 2017-03-29 2020-06-11 Editas Medicine, Inc. Nucleic acid mutagenesis methods
US11299776B2 (en) * 2017-05-10 2022-04-12 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2019143622A1 (fr) * 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Adn polymérases mutantes
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
CA3175648A1 (fr) 2020-04-30 2021-11-04 Alcino Orfeu De Leao E Flores Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (fr) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Procédés de purification de polypeptides
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

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US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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FR2652520B1 (fr) 1989-10-02 1992-02-07 Sgn Soc Gen Tech Nouvelle Procede et dispositif pour maintenir une atmosphere propre a temperature regulee sur un poste de travail.
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EP0894860B1 (fr) 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG DNA polymérases thermostables et mutant de délétions
JPH06502303A (ja) 1990-10-05 1994-03-17 バーネス，ウェーン・エム 熱安定性ｄｎａポリメラーゼ
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
CA2222744C (fr) 1995-05-31 2008-03-25 Amersham Life Science, Inc. Adn-polymerases thermostables
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
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GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
JP4768155B2 (ja) 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
CA2409775C (fr) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Enzymes thermostables modifies de facon reversible pour la synthese de l'adn et l'amplification in vitro

Also Published As

Publication number Publication date
HK1074458A1 (en) 2005-11-11
EP1507002A2 (fr) 2005-02-16
CN1590543B (zh) 2010-05-12
ATE348881T1 (de) 2007-01-15
KR100626578B1 (ko) 2006-09-25
BRPI0403221A (pt) 2005-07-12
JP2005058236A (ja) 2005-03-10
MXPA04007721A (es) 2005-07-12
DE602004003755D1 (de) 2007-02-01
EP1507002A3 (fr) 2005-07-27
DK1507002T3 (da) 2007-04-10
AU2004203649A1 (en) 2005-03-03
KR20050025266A (ko) 2005-03-14
US20050037412A1 (en) 2005-02-17
US7972830B2 (en) 2011-07-05
CA2474734C (fr) 2010-07-13
ES2278249T3 (es) 2007-08-01
CA2474734A1 (fr) 2005-02-12
CN1590543A (zh) 2005-03-09
DE602004003755T2 (de) 2007-10-11
AU2004203649B2 (en) 2006-01-12
EP1507002B1 (fr) 2006-12-20
JP3929061B2 (ja) 2007-06-13

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