CA3175648A1 - Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus - Google Patents

Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus

Info

Publication number
CA3175648A1
CA3175648A1 CA3175648A CA3175648A CA3175648A1 CA 3175648 A1 CA3175648 A1 CA 3175648A1 CA 3175648 A CA3175648 A CA 3175648A CA 3175648 A CA3175648 A CA 3175648A CA 3175648 A1 CA3175648 A1 CA 3175648A1
Authority
CA
Canada
Prior art keywords
portable device
samples
amplification
dna
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3175648A
Other languages
English (en)
Inventor
Alcino Orfeu De Leao E Flores
Goncalo Maria Reimao Pinto De Franca Doria
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STAB VIDA Investigacao e Servicos em Ciencias Biologicas Lda
Original Assignee
STAB VIDA Investigacao e Servicos em Ciencias Biologicas Lda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by STAB VIDA Investigacao e Servicos em Ciencias Biologicas Lda filed Critical STAB VIDA Investigacao e Servicos em Ciencias Biologicas Lda
Publication of CA3175648A1 publication Critical patent/CA3175648A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/023—Sending and receiving of information, e.g. using bluetooth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un procédé et un dispositif portatif (1) servant à la détection et l'identification de séquences spécifiques d'acides nucléiques dans différents échantillons, par l'utilisation d'une technique de transcription inverse et/ou d'une amplification isotherme, à l'aide d'amorces oligonucléotidiques spécifiques d'une ou plusieurs régions cibles à détecter, grâce à quoi leur amplification est réalisée sous forme indirecte et non spécifique, ou directe et spécifique, à l'aide de réactifs d'amplification discriminatoires, dont le signal, colorimétrique/fluorescent, est enregistré par le dispositif (1) où la réaction a lieu. Le dispositif est commandé par une application mobile (9), enregistrant également des données acquises par des analyses du dispositif (1), les mémorise dans un serveur distant en nuage (10), qui analyse un ensemble global de toutes les données, ce qui permet une analyse globale, par exemple, de l'évolution d'épidémies. L'invention est utile pour la détection et l'identification de séquences nucléiques pertinentes pour différents types de diagnostic dans différents domaines cliniques, pharmaceutiques, vétérinaires, alimentaires, environnementaux, biotechnologiques, et de biosécurité, et est particulièrement utile dans le diagnostique rapide et peu coûteux, et sur le lieu d'intervention, du virus SARS-CoV-2.
CA3175648A 2020-04-30 2021-04-28 Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus Pending CA3175648A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PT11631720 2020-04-30
PT116317 2020-04-30
PCT/IB2021/053530 WO2021220192A2 (fr) 2020-04-30 2021-04-28 Procédé et dispositif portatif pour la détection de séquences nucléiques dans des échantillons suspectés d'être infectés par le coronavirus

Publications (1)

Publication Number Publication Date
CA3175648A1 true CA3175648A1 (fr) 2021-11-04

Family

ID=75787159

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3175648A Pending CA3175648A1 (fr) 2020-04-30 2021-04-28 Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus

Country Status (8)

Country Link
EP (1) EP4142945A2 (fr)
JP (1) JP2023524117A (fr)
CN (1) CN115836133A (fr)
AU (1) AU2021265579A1 (fr)
BR (1) BR112022021086A2 (fr)
CA (1) CA3175648A1 (fr)
IL (1) IL297281A (fr)
WO (1) WO2021220192A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES1298031Y (es) * 2022-02-11 2023-06-02 Consorcio De Aguas De Asturias Sistema de analisis de aguas residuales o ambientales para la deteccion de virus, bacterias o microorganismos eucariotas
WO2024047567A1 (fr) * 2022-08-31 2024-03-07 VD-ING d.o.o. Procédé, dispositif et système de détection d'une infection microbienne ou virale
KR20240049694A (ko) * 2022-10-07 2024-04-17 주식회사 원드롭 다수의 측정기에서 이루어진 핵산 증폭 결과 데이터를 효율적으로 전송하는 방법 및 장치

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
PT103729A (pt) * 2007-05-04 2008-11-04 Stab Vida Investigacao E Servi Estojo e processo para a detecção e identificação de leveduras de interesse clínico, através de uma amplificação isotérmica de adn seguida de hibridação com oligonucleótidos específicos, e respectivas utilizações.
WO2010141940A1 (fr) 2009-06-05 2010-12-09 Alere San Diego, Inc. Réactifs et kits d'amplification par recombinase polymérase
WO2011156838A1 (fr) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Dispositif de laboratoire sur puce pour détection d'agents pathogènes avec dialyse, lyse chimique et amplification d'acides nucléiques en tandem
JP6538691B2 (ja) * 2013-08-26 2019-07-03 ダイアジェネティクス・インコーポレイテッド 核酸増幅のための携帯機器の動作用ハードウェア及びモバイルソフトウェア
ITUB20159332A1 (it) 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
US20170276599A1 (en) * 2016-03-24 2017-09-28 The Board Of Regents Of The University Of Texas System Cost effective battery-powered spectrophotometric system

Also Published As

Publication number Publication date
CN115836133A (zh) 2023-03-21
WO2021220192A4 (fr) 2022-02-03
BR112022021086A2 (pt) 2022-12-13
AU2021265579A1 (en) 2022-11-10
IL297281A (en) 2022-12-01
EP4142945A2 (fr) 2023-03-08
JP2023524117A (ja) 2023-06-08
WO2021220192A3 (fr) 2021-12-09
WO2021220192A2 (fr) 2021-11-04

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