DK1507002T3 - Termostabilt Taq-polymerasefragment - Google Patents

Termostabilt Taq-polymerasefragment

Info

Publication number
DK1507002T3
DK1507002T3 DK04018913T DK04018913T DK1507002T3 DK 1507002 T3 DK1507002 T3 DK 1507002T3 DK 04018913 T DK04018913 T DK 04018913T DK 04018913 T DK04018913 T DK 04018913T DK 1507002 T3 DK1507002 T3 DK 1507002T3
Authority
DK
Denmark
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
DK04018913T
Other languages
Danish (da)
English (en)
Inventor
Waltraud Dr Ankenbauer
Thomas Dr Meier
Annette Dr Deufel
Dieter Dr Heindl
Gisela Dr Betzl
Rainer Dr Schmuck
Bernd Dr Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1507002T3 publication Critical patent/DK1507002T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK04018913T 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment DK1507002T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
DK1507002T3 true DK1507002T3 (da) 2007-04-10

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04018913T DK1507002T3 (da) 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment

Country Status (15)

Country Link
US (1) US7972830B2 (fr)
EP (1) EP1507002B1 (fr)
JP (1) JP3929061B2 (fr)
KR (1) KR100626578B1 (fr)
CN (1) CN1590543B (fr)
AT (1) ATE348881T1 (fr)
AU (1) AU2004203649B2 (fr)
BR (1) BRPI0403221A (fr)
CA (1) CA2474734C (fr)
DE (1) DE602004003755T2 (fr)
DK (1) DK1507002T3 (fr)
ES (1) ES2278249T3 (fr)
HK (1) HK1074458A1 (fr)
MX (1) MXPA04007721A (fr)
SG (1) SG109552A1 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008526216A (ja) * 2005-01-04 2008-07-24 ストラタジーン カリフォルニア 熱不安定性阻害剤を使用するホットスタートポリメラーゼ反応
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (fr) 2008-04-28 2009-11-04 Biotype AG Substances et procédés pour un dosage de profilage à base d'ADN
EP2644708B1 (fr) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. Dosages à base de rnase-h utilisant des monomères d'arn modifiés
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
JP5809059B2 (ja) 2008-11-03 2015-11-10 カパ バイオシステムズ， インコーポレイテッド 改変ａ型ｄｎａポリメラーゼ
KR20170098985A (ko) 2009-11-19 2017-08-30 솔리스 바이오다인 폴리펩티드 안정성 및 활성을 증가시키는 조성물 및 관련된 방법
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012146260A1 (fr) 2011-04-23 2012-11-01 Biolytix Ag Préparation et utilisation de protéines en biologie moléculaire
DK3461807T3 (da) 2011-06-08 2023-09-04 Life Technologies Corp Design og udvikling af hidtil ukendte detergenter til anvendelse i pcr-systemer
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
US9914964B2 (en) 2013-10-25 2018-03-13 Life Technologies Corporation Compounds for use in PCR systems and applications thereof
CN113403293B (zh) * 2014-12-16 2024-08-16 生命技术公司 聚合酶组合物和制造与使用其的方法
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
US11046939B2 (en) * 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
US20200181666A1 (en) * 2017-03-29 2020-06-11 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (fr) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Procédés et dispositifs associés à l'amplification d'acides nucléiques à diverses températures
CN111684064A (zh) 2018-01-19 2020-09-18 生物辐射实验室股份有限公司 突变dna聚合酶
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
CA3175648A1 (fr) 2020-04-30 2021-11-04 Alcino Orfeu De Leao E Flores Procede et dispositif portatif pour la detection de sequences nucleiques dans des echantillons suspectes d'etre infectes par le coronavirus
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (fr) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Procédés de purification de polypeptides
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
CA1284931C (fr) 1986-03-13 1991-06-18 Henry A. Erlich Procede de detection de variations specifiques de nucleotides et de polymorphismes genetiques dans les acides nucleiques
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
CA1338457C (fr) 1986-08-22 1996-07-16 Henry A. Erlich Enzyme thermostable purifiee
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
FR2652520B1 (fr) 1989-10-02 1992-02-07 Sgn Soc Gen Tech Nouvelle Procede et dispositif pour maintenir une atmosphere propre a temperature regulee sur un poste de travail.
AU6735490A (en) 1989-10-16 1991-05-16 E.I. Du Pont De Nemours And Company Process for chlorofluoropropanes
AU663474B2 (en) * 1990-09-28 1995-10-12 F. Hoffmann-La Roche Ag 5' to 3' exonuclease mutations of thermostable DNA polymerases
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
JP3966555B2 (ja) 1995-05-31 2007-08-29 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 熱耐性ｄｎａポリメラーゼ
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
DK1069131T3 (da) 1999-07-15 2006-06-26 Qiagen Gmbh Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler
GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
JP4768155B2 (ja) 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
CA2409775C (fr) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Enzymes thermostables modifies de facon reversible pour la synthese de l'adn et l'amplification in vitro

Also Published As

Publication number Publication date
KR20050025266A (ko) 2005-03-14
CN1590543A (zh) 2005-03-09
JP3929061B2 (ja) 2007-06-13
CA2474734A1 (fr) 2005-02-12
CA2474734C (fr) 2010-07-13
BRPI0403221A (pt) 2005-07-12
MXPA04007721A (es) 2005-07-12
CN1590543B (zh) 2010-05-12
EP1507002B1 (fr) 2006-12-20
EP1507002A3 (fr) 2005-07-27
ATE348881T1 (de) 2007-01-15
JP2005058236A (ja) 2005-03-10
US7972830B2 (en) 2011-07-05
DE602004003755T2 (de) 2007-10-11
AU2004203649A1 (en) 2005-03-03
ES2278249T3 (es) 2007-08-01
KR100626578B1 (ko) 2006-09-25
AU2004203649B2 (en) 2006-01-12
SG109552A1 (en) 2005-03-30
HK1074458A1 (en) 2005-11-11
US20050037412A1 (en) 2005-02-17
DE602004003755D1 (de) 2007-02-01
EP1507002A2 (fr) 2005-02-16

Similar Documents

Publication Publication Date Title
HK1074458A1 (en) Thermostable taq polymerase fragment
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DE602004025801D1 (de) Dna-polymerasefusionen und deren verwendungen
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
ATE364718T1 (de) Verfahren zur vervielfältigung von nukleinsäure
DK0550687T3 (da) 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser
ATE325877T1 (de) Mutierte chimäre dns-polymerasen
ATE302279T1 (de) Kälteempfindliche dna-polymerasemutanten
DE60110564D1 (en) Rna polymerase mutanten mit erhöhter thermostabilität
RS92704A (en) Immunoglobulin-domain containing cell surface recognition molecules
HUP0303517A2 (hu) Hímfertilitást közvetítő nukleotidszekvenciák és alkalmazásuk
DE60301325D1 (de) Mutation von dna polymerasen aus archaeobakterien
ATE423785T1 (de) Verfahren und zusammensetzungen zur identifikation und charakterisierung von hepatitis c
DE602005023586D1 (de) Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'-nichttranslatierten bereichs von hcv
ATE496122T1 (de) Thermostabile polymerase aus i thermococcus pacificus /i
Ikeuchi et al. The enhancing of a cysteine proteinase activity at acidic pH by protein engineering, the role of glutamic 50 in the enzyme mechanism of caricain
DK2186898T3 (da) Blomstrings-induktion
IL195194A0 (en) Thermostable dna polymerase of the archaeal ampullavirus abv and its applications
DK1789541T3 (da) Overeksprimeret og renset Aspergillus ficuum-oxidase og nukleinsyre, der koder derfor
DE602006005932D1 (de) Verfahren zur identifizierung von proteinfaltungsinhibitoren