SG10201803961VA - Cell culture and gradient migration assay methods and devices - Google Patents

Cell culture and gradient migration assay methods and devices

Info

Publication number
SG10201803961VA
SG10201803961VA SG10201803961VA SG10201803961VA SG10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA
Authority
SG
Singapore
Prior art keywords
devices
cell culture
migration assay
assay methods
gradient migration
Prior art date
Application number
SG10201803961VA
Other languages
English (en)
Inventor
Ju-Sung HUNG
Philip Lee
Amedeo Cappione
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/436,992 external-priority patent/US9637715B2/en
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG10201803961VA publication Critical patent/SG10201803961VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG10201803961VA 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices SG10201803961VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/436,992 US9637715B2 (en) 2005-07-07 2012-04-01 Cell culture and invasion assay method and system
US201361761227P 2013-02-05 2013-02-05

Publications (1)

Publication Number Publication Date
SG10201803961VA true SG10201803961VA (en) 2018-07-30

Family

ID=49300898

Family Applications (3)

Application Number Title Priority Date Filing Date
SG11201405210QA SG11201405210QA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices
SG10201803961VA SG10201803961VA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201405210QA SG11201405210QA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices

Country Status (5)

Country Link
EP (1) EP2834649A4 (ja)
JP (2) JP2015517804A (ja)
CN (2) CN104412109A (ja)
SG (3) SG11201405210QA (ja)
WO (1) WO2013151616A1 (ja)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2992206B1 (fr) * 2012-06-21 2014-07-18 Oreal Composition cosmetique comprenant une huile, des particules d'aerogel de silice hydrophobe et une resine hydrocarbonee
SG10201806083RA (en) 2013-02-22 2018-08-30 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
ES2717927T3 (es) 2013-02-22 2019-06-26 Genzyme Corp Procedimientos de cultivo por perfusión de microportadores y usos de los mismos
JP6486898B2 (ja) * 2014-02-25 2019-03-20 国立大学法人京都大学 マイクロ流体デバイス及び細胞の微小３次元培養法
TWI743024B (zh) * 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI707949B (zh) 2014-06-09 2020-10-21 美商健臻公司 種子罐培養法（ｓｅｅｄ ｔｒａｉｎ ｐｒｏｃｅｓｓｅｓ）及其用途
US11306341B2 (en) 2014-12-22 2022-04-19 Genzyme Corporation Methods of culturing a mammalian cell
CN105062879B (zh) * 2015-07-26 2017-06-27 江苏大学附属医院 细胞多向迁移实验用装置
CN105062890A (zh) * 2015-09-11 2015-11-18 温州梅塔光学科技有限公司 一种单细胞实验装置
NL2016404B1 (en) 2016-03-09 2017-09-26 Mimetas B V Double tubular structures.
BR112018075920A2 (pt) 2016-06-15 2019-03-26 Mimetas B.V. dispositivo e métodos de cultura celular
CN109475864B (zh) * 2016-06-29 2022-03-04 美天施生物科技有限两合公司 用于生物试样的多级一次性筒
WO2018234163A1 (en) * 2017-06-16 2018-12-27 İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi METHOD FOR DETERMINING CELL MIGRATION AND INVASION
JP6812944B2 (ja) * 2017-10-18 2021-01-13 株式会社島津製作所 質量分析装置用情報管理装置
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108641931A (zh) * 2018-04-04 2018-10-12 浙江大学 一种数字化微阵列器官芯片及其应用
CN111057639B (zh) * 2019-11-29 2022-06-17 中国医科大学 一种基于微流控芯片的循环肿瘤细胞分离富集系统和方法
EP4177350A1 (en) * 2020-07-06 2023-05-10 Nikon Corporation Migration ability calculation device, migration ability evaluation method, and computer program for causing computer to execute migration ability evaluation method
WO2022018743A1 (en) * 2020-07-22 2022-01-27 INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) A system and method for simultaneous live-cell imaging and growing
CN112646713A (zh) * 2020-12-25 2021-04-13 中国科学院广州生物医药与健康研究院 一种整合性肿瘤细胞行为实验用芯片
NL2028424B1 (en) 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113648287B (zh) * 2021-07-30 2023-05-05 深圳华源再生医学有限公司 细胞装载装置及其制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1545750A4 (en) * 2002-08-27 2007-09-19 Univ Vanderbilt BIOREACTORS WITH POSSIBILITIES OF INJECTION OF SUBSTANCES
WO2005059088A1 (en) * 2003-12-19 2005-06-30 University Of Waterloo Cultured cell and method and apparatus for cell culture
JP2008519598A (ja) * 2004-11-11 2008-06-12 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 細胞培養デバイス
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US8377685B2 (en) * 2007-11-07 2013-02-19 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

Also Published As

Publication number Publication date
EP2834649A1 (en) 2015-02-11
SG11201405210QA (en) 2014-09-26
SG10201607963SA (en) 2016-11-29
EP2834649A4 (en) 2015-12-09
CN104412109A (zh) 2015-03-11
WO2013151616A1 (en) 2013-10-10
JP2015517804A (ja) 2015-06-25
JP2017093467A (ja) 2017-06-01
CN106399094A (zh) 2017-02-15

Similar Documents

Publication Publication Date Title
SG10201803961VA (en) Cell culture and gradient migration assay methods and devices
EP3019588A4 (en) Systems and methods for cell culture device interconnection and fluidic device interconnection
EP3194560A4 (en) Microfluidic system and method for perfusion bioreactor cell retention
HK1222672A1 (zh) 用於處理細胞培養物的方法和系統
DK2870013T3 (da) System og fremgangsmåde til styring af drift af et metal-luft-batteri
EP3004320A4 (en) Closed system device and methods for gas permeable cell culture process
SG10201604400RA (en) Micro-incubation systems for microfluidic cell culture and methods
DK2968415T3 (da) Cd123-specifik kimær antigenreceptor-omdirigerede t-celler og fremgangsmåder til anvendelse deraf
MX2013008538A (es) Amplificador compensado de fijacion de membrana para la secuenciacion de polinucleotido por nanoporo y otras aplicaciones.
EP2987851A4 (en) Microfluidic multi-well-based cell culture test equipment
GB2517864A (en) Electrophoretic separation devices and methods for using the same
MY173953A (en) Recombinant host cells for the production of malonate
MX2015015406A (es) Matrices de micro-entornos de cultivos celulares discretos, metodos para producir estas matrices y usos de las mismas.
UY34317A (es) Anticuerpo antireceptor de célula T (alfa)/ß
DK3253863T3 (da) Bioreaktorsystem til celledyrkning
EP3060648A4 (en) Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
HK1213285A1 (zh) 用於優化灌流細胞培養系統的方法和系統
EP3392332A4 (en) DEVICE AND SYSTEM FOR CELL CULTURE
WO2014130864A3 (en) Microcarrier perfusion culturing methods and uses thereof
EP3170892A4 (en) Method for enhancing activity of laminin fragment cell culture substrate
EP3027730A4 (en) MICROFLUIDIC SYSTEMS OF CELL CULTURE
DK2766468T3 (da) Fremgangsmåde til kontrolleret drift af en bioteknologisk indretning og bioreaktorsystemer
MX2019009189A (es) Metodos de cultivo por perfusion con microportadores y usos de los mismos.
MY158171A (en) Control device for information processing device
EP2963071A4 (en) Novel graft polymer, temperature-responsive substrate for cell culture using same and production method therefor, and liquid chromatography carrier having immobilized novel graft polymer and liquid chromatography method using same