RU2713298C2 - Способ получения l-аминокислот с помощью коринебактерий с применением системы расщепления глицина - Google Patents

Способ получения l-аминокислот с помощью коринебактерий с применением системы расщепления глицина Download PDF

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RU2713298C2
RU2713298C2 RU2016143585A RU2016143585A RU2713298C2 RU 2713298 C2 RU2713298 C2 RU 2713298C2 RU 2016143585 A RU2016143585 A RU 2016143585A RU 2016143585 A RU2016143585 A RU 2016143585A RU 2713298 C2 RU2713298 C2 RU 2713298C2
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Инес ОХРОМБЕЛЬ
Бриджитт БАТЭ
Марлен ХАССЕЛЬМАЙЕР
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Эвоник Оперейшенс ГмбХ
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Abstract

Группа изобретений относится к получению L-аминокислот. Предложена система расщепления глицина, содержащая ферменты GcvP, GcvT и GcvH, указанная система содержит по меньшей мере один из следующих полипептидов: фермент GcvP с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 40, фермент GcvТ с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 42, фермент GcvН с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 38. Предложены также полипептид, который представляет собой фермент системы расщепления глицина, полинуклеотид, который кодирует фермент системы расщепления глицина, экспрессионный вектор, содержащий по меньшей мере один указанный полинуклеотид. Предложен также способ получения в избыточном количестве L-аминокислоты, в котором применяют указанную систему расщепления глицина, и/или по меньшей мере один указанный полипептид, и/или по меньшей мере один указанный полинуклеотид, и/или указанный вектор. Группа изобретений обеспечивает эффективное получение L-аминокислоты, выбранной из L-аланина, L-валина и L-аминокислот семейства глутамата. 5 н. и 4 з.п. ф-лы, 1 табл., 2 пр.

Description

Настоящее изобретение относится к способу получения L-аминокислот с помощью коринебактерий, в котором используется система расщепления глицина.
Способы получения L-аминокислот, в которых используют бактерии рода Corynebacterium, известны специалистам в данной области.
Хотя известно множество видов коринебактерий, в данных способах обычно используют бактерии вида Corynebacterium glutamicum, поскольку было установлено, что этот вид является особенно предпочтительным для получения L-аминокислот.
Также было установлено, что выход L-аминокислоты можно дополнительно увеличить путем использования системы расщепления глицина (GCV), поскольку образования нежелательного побочного продукта, представляющего собой L-глицин, можно в значительной степени избежать с помощью системы расщепления глицина. Подавление образования L-глицина или, в частности, расщепление избытка L-глицина имеет особое значение в получении L-метионина, поскольку L-глицин в данном случае образуется в виде побочного продукта в эквимолярном количестве.
Система расщепления глицина состоит из двух или более субъединиц, а именно субъединиц GcvP, GcvT и GcvH. Она имеет форму полиферментного комплекса, который катализирует окислительное декарбоксилирование и дезаминирование глицина до диоксида углерода, ионов аммония и N5-10-метилентетрагидрофолата.
Глициндегидрогеназа GcvP представляет собой пиридоксальфосфат-содержащую декарбоксилазу, которая высвобождает диоксид углерода и оставляет соединение аминометила связанным с пиридоксальфосфатом.
H-белок в GcvH представляет собой липоамид-содержащую аминометилтрансферазу.
Фермент GcvT катализирует нуклеофильную атаку на аминометилипоамид посредством тетрагидрофолата с образованием N5-10-метилентетрагидрофолата и высвобождением ионов аммония, при этом полностью восстановленный липоамид остается связанным с GcvH.
Системы расщепления глицина встречаются не у всех коринебактерий, и до настоящего времени были описаны лишь у незначительного количества коринебактерий. Например, у особенно предпочтительного для получения аминокислот штамма-продуцента C.glutamicum отсутствует собственная система расщепления глицина. Чтобы использовать преимущества системы расщепления глицина для C. glutamicum, такую систему необходимо встроить из другой коринебактерии в C.glutamicum и гетерологично экспрессировать. До настоящего времени для этой цели использовали систему расщепления глицина из C.jeikeium (WO 2008/101857). В данном случае серьезным недостатком было то, что C. jeikeium является патогенным организмом. Другой недостаток заключался в том, что для расщепления нежелательного побочного продукта, представляющего собой глицин, необходимо было получить гетерологичную конструкцию.
Таким образом, основной целью настоящего изобретения было обеспечение новой системы расщепления глицина, предпочтительно системы расщепления глицина, которая происходит не из патогенного организма, где система расщепления глицина предпочтительно является подходящей для встраивания в другие коринебактерии, в частности в C.glutamicum.
Дополнительной целью настоящего изобретения было обеспечение коринебактерии, которой свойственно продуцирование L-аминокислот, и которая, кроме того, имеет собственную систему расщепления глицина, вследствие чего создание гетерологичной конструкции, как в случае с C.glutamicum, не является необходимостью.
В соответствии с настоящим изобретением неожиданно было установлено, что вид Corynebacterium humireducens, который не является патогенным, имеет собственную систему расщепления глицина, которая по своей структуре в значительной степени отличается от систем расщепления глицина, описанных до настоящего времени.
Неожиданно дополнительно было установлено, что система расщепления глицина из штамма Corynebacterium humireducens дикого типа является настолько эффективной, что глицин, представляющий собой нежелательный побочный продукт в ходе синтеза аминокислот, не накапливается в клетке или накапливается лишь в относительно незначительных количествах. Путем усиления системы расщепления глицина в соответствии с настоящим изобретением, в особенности путем сверхэкспрессии, подавление образования побочного продукта, представляющего собой глицин, может быть соответствующим образом дополнительно улучшено. В частности, образование побочного продукта, представляющего собой глицин, можно также эффективно подавлять посредством сверхэкспрессии у других микроорганизмов системы расщепления глицина в соответствии с настоящим изобретением, в частности у C.glutamicum.
Кроме того, неожиданно было установлено, что C.humireducens уже по своей природе способен продуцировать L-аминокислоты, в частности L-аланин, L-валин и L-глутаминовую кислоту, для своих собственных потребностей, то есть C.humireducens представляет собой штамм, продуцирующий L-аминокислоты, с гомологичной системой расщепления глицина, которую можно использовать, в частности, в качестве отправной точки для разработки других штаммов-продуцентов с собственной системой расщепления глицина.
Штамм C. humireducens был впервые описан в публикации Wu и соавт. (International Journal of Systematic and Evolutionary Microbiology (2011), 61, 882-887). Указанный штамм депонировали в DSMZ под номером доступа DSM 45392, а его 16S rRNA депонировали в EMBL под номером доступа GQ421281. Родительский штамм представляет собой галотолерантную, алкалифильную, восстанавливающую гуминовые кислоты бактерию.
Дополнительную информацию относительно C.humireducens можно найти в следующих публикациях: Wu и соавт. (Microb. Biotechnol. (2013), 6(2), 141-149), Lin et al. (Bioresour. Technol. (2013), 136, 302-308).
Таким образом, основная цель настоящего изобретения касается ферментов системы расщепления глицина, выбранных из группы, включающей:
a) фермент GcvP с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvT с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 42,
с) фермент GcvH с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 38.
Таким образом, настоящее изобретение дополнительно относится к системе расщепления глицина, содержащей ферменты GcvP, GcvT и GcvH, которая характеризуется тем, что указанная система содержит по меньшей мере один из следующих полипептидов:
a) фермент GcvP с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvT с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 42,
c) фермент GcvH с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 38.
В данном случае система расщепления глицина в соответствии с настоящим изобретением предпочтительно содержит по меньшей мере два, предпочтительно все три из упомянутых выше полипептидов (a)-(c).
Особенное предпочтение отдают системе расщепления глицина, содержащей три следующих полипептида:
a) фермент GcvP с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvT с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 42,
c) фермент GcvH с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 38.
В более широком смысле ферменты LpdA, LplA, LipA, LipB и GcvH также относятся к системе расщепления глицина.
Дигидролипоамиддегидрогеназа (LpdA) повторно окисляет липоамид, связанный с GcvH.
Липоатпротеинлигаза A (LplA) катализирует липоилирование GcvH.
Синтаза липоевой кислоты (LipA) катализирует синтез липоевой кислоты.
Липоил-[белок-переносчик ацила]-протеин-N-липоилтрансфераза (LipB) катализирует перенос липоевой кислоты к GcvH.
GcvL представляет собой дигидролипоилдегидрогеназу.
Следовательно, в предпочтительном варианте осуществления система расщепления глицина в соответствии с настоящим изобретением дополнительно содержит по меньшей мере один дополнительный фермент, выбранный из группы, включающей:
a) фермент LipA, предпочтительно фермент LipA с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 48,
b) фермент LipB, предпочтительно фермент LipB с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 50,
c) фермент Lpd, предпочтительно фермент Lpd с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 52,
d) фермент LplA, предпочтительно фермент LplA с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 94,
e) фермент GcvL, предпочтительно фермент GcvL с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 96.
В особенно предпочтительном варианте осуществления в соответствии с настоящим изобретением система расщепления глицина в соответствии с настоящим изобретением в данном случае содержит по меньшей мере два, три или четыре, предпочтительно все пять из упомянутых выше полипептидов.
Таким образом, настоящее изобретение дополнительно относится к ферментам, выбранным из группы, включающей:
a) фермент LipA, предпочтительно фермент LipA с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 48,
b) фермент LipB, предпочтительно фермент LipB с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 50,
c) фермент Lpd, предпочтительно фермент Lpd с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 52,
d) фермент LplA, предпочтительно фермент LplA с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 94,
e) фермент GcvL, предпочтительно фермент GcvL с последовательностью, которая по меньшей мере на 80%, предпочтительно по меньшей мере на 85 или 90%, в частности по меньшей мере на 92, 94, 96 или 98%, в особенности на 100% идентична последовательности под SEQ ID NO: 96.
В качестве альтернативы или в качестве дополнения к применению соответствующих ферментов, которые обеспечивают эффективный синтез липоевой кислоты и/или липоамида, данные соединения можно также добавлять в реакционную среду, например, в количествах не более 15 мМ.
Настоящее изобретение также дополнительно относится к полинуклеотидам, кодирующим ферменты в соответствии с настоящим изобретением и/или систему расщепления глицина в соответствии с настоящим изобретением. Данные полинуклеотиды предпочтительно выбраны группы, включающей:
a) полинуклеотид (gcvP), который кодирует фермент GcvP и имеет последовательность, которая по меньшей мере на 70 или 75%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-3162 под SEQ ID NO: 39, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-3162 под SEQ ID NO: 39;
b) полинуклеотид (gcvT), который кодирует фермент GcvT и имеет последовательность, которая по меньшей мере на 70 или 75%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1404, под SEQ ID NO: 41, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1404 под SEQ ID NO: 41;
c) полинуклеотид (gcvH), который кодирует фермент GcvH и имеет последовательность, которая по меньшей мере на 70 или 75%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-699 под SEQ ID NO: 37, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-699 под SEQ ID NO: 37;
d) полинуклеотид (lipA), который кодирует фермент LipA и имеет последовательность, которая по меньшей мере на 70%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1344 под SEQ ID NO: 47, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1344 под SEQ ID NO: 47;
e) полинуклеотид (lipB), который кодирует фермент LipB и имеет последовательность, которая по меньшей мере на 70%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1089 под SEQ ID NO: 49, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1089 под SEQ ID NO: 49;
f) полинуклеотид (lpd), который кодирует фермент Lpd и имеет последовательность, которая по меньшей мере на 70%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1710 под SEQ ID NO: 51, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1710 под SEQ ID NO: 51;
g) полинуклеотид (IpIA), который кодирует фермент LplA и имеет последовательность, которая по меньшей мере на 70%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1089 под SEQ ID NO: 93, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1089 под SEQ ID NO: 93;
h) полинуклеотид (gcvL), который кодирует фермент GcvL и имеет последовательность, которая по меньшей мере на 70%, предпочтительно по меньшей мере на 80 или 85%, в частности по меньшей мере на 90 или 95%, в особенности по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1710 под SEQ ID NO: 95, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1710 под SEQ ID NO: 95.
В соответствии с настоящим изобретением под «жесткими условиями» следует понимать отмывание при концентрации солей 1 x SSC и 0,1% по весу SDS при температуре 80°C.
Настоящее изобретение также дополнительно относится к полинуклеотидам, которые комплементарны кодирующим полинуклеотидам, упомянутым выше.
Настоящее изобретение дополнительно относится к соответствующим векторам, в частности клонирующим и экспрессионным векторам, содержащим по меньшей мере один полинуклеотид в соответствии с настоящим изобретением. Данные векторы можно встраивать соответствующим образом в микроорганизмы, в частности в коринеформные бактерии, в особенности рода Corynbebacterium, или энтеробактерии, в особенности рода Escherichia.
Векторы в соответствии с настоящим изобретением могут содержать один или более полинуклеотидов в соответствии с настоящим изобретением. Предпочтительный вектор в соответствии с настоящим изобретением содержит по меньшей мере один полинуклеотид, кодирующий фермент в соответствии с настоящим изобретением, выбранный из GcvP, GcvT и GcvH. Особенно предпочтительный вектор содержит полинуклеотиды, которые кодируют все три фермента GcvP, GcvT и GcvH в соответствии с настоящим изобретением.
Векторы в соответствии с настоящим изобретением предпочтительно имеют подходящий промотор и/или подходящие промоторы и необязательно дополнительные регуляторные элементы, которые обеспечивают экспрессию полинуклеотидов по настоящему изобретению в рекомбинантной бактерии, предпочтительно в рекомбинантной коринебактерии.
Кроме того, полинуклеотиды в соответствии с настоящим изобретением с целью экспрессии кодируемых генов можно также встраивать в геном микроорганизмов, в частности, в геном коринеформных бактерий, в особенности бактерий рода Corynebacterium, или в геном энтеробактерий, в особенности рода Escherichia.
Настоящее изобретение также дополнительно относится к соответствующим рекомбинантным микроорганизмам, предпочтительно к бактериям, в частности, к коринеформным бактериям, в особенности бактериям рода Corynebacterium, в особенности предпочтительно к видам C.humireducens или C.glutamicum, а также к энтеробактериям, в особенности к бактериям рода Escherichia, содержащим по меньшей мере один фермент в соответствии с настоящим изобретением, и/или по меньшей мере один полинуклеотид в соответствии с настоящим изобретением, и/или по меньшей мере один вектор в соответствии с настоящим изобретением, и/или одну систему расщепления глицина в соответствии с настоящим изобретением, и/или полинуклеотид, кодирующий систему расщепления глицина в соответствии с настоящим изобретением.
В этом отношении предпочтительным объектом являются рекомбинантные коринебактерии, в частности, бактерии видов C.humireducens и видов C.glutamicum, содержащие по меньшей мере один фермент в соответствии с настоящим изобретением, предпочтительно все ферменты в соответствии с настоящим изобретением, выбранные из GcvP, GcvT и GcvH, и/или полинуклеотиды, кодирующие указанные ферменты, и/или по меньшей мере один вектор, содержащий указанные полинуклеотиды.
В частности, настоящее изобретение также относится к рекомбинантным микроорганизмам, предпочтительно к бактериям, в частности, к коринеформным бактериям, в особенности бактериям рода Corynebacterium, кроме вида C.humireducens, в частности, к тем видам C.glutamicum, которые содержат по меньшей мере один фермент в соответствии с настоящим изобретением, и/или по меньшей мере один полинуклеотид в соответствии с настоящим изобретением, и/или по меньшей мере один вектор в соответствии с настоящим изобретением, и/или одну систему расщепления глицина в соответствии с настоящим изобретением, и/или полинуклеотид, кодирующий систему расщепления глицина в соответствии с настоящим изобретением.
В этом отношении предпочтительным объектом являются рекомбинантные коринебактерии, за исключением бактерий вида C.humireducens, в частности, вида C.glutamicum, которые содержат по меньшей мере один фермент в соответствии с настоящим изобретением, предпочтительно все ферменты в соответствии с настоящим изобретением, выбранные из GcvP, GcvT и GcvH, и/или полинуклеотиды, кодирующие указанные ферменты, и/или по меньшей мере один вектор, содержащий указанные полинуклеотиды.
В соответствии с настоящим изобретением «рекомбинантный микроорганизм» или «рекомбинантную бактерию» необходимо понимать как микроорганизм или бактерию, которых подвергали по меньшей мере одной генноинженерной манипуляции. В этом отношении генноинженерная манипуляция может представлять собой, в частности, целенаправленную или случайную мутацию, встраивание чужеродного гена и/или сверхэкспрессию или аттенуацию экспрессии гена хозяина или чужеродного гена. Рекомбинантный микроорганизм в соответствии с настоящим изобретением или рекомбинантная бактерия в соответствии с настоящим изобретением предпочтительно характеризуется сверхэкспрессией или аттенуацией по меньшей мере одного гена. В особенно предпочтительном варианте осуществления рекомбинантный микроорганизм в соответствии с настоящим изобретением или рекомбинантная бактерия в соответствии с настоящим изобретением характеризуется сверхэкспрессией по меньшей мере одного фермента в соответствии с настоящим изобретением и/или полинуклеотида, кодирующего указанный фермент, и/или сверхэкспрессией системы расщепления глицина в соответствии с настоящим изобретением, или полинуклеотида, кодирующего указанную систему.
«Относительно незначительное количество» по отношению к образованию глицина необходимо понимать как количество, которое составляет не более 0,3 г/л, предпочтительно не более 0,2 или 0,1 г/л, в частности, предпочтительно не более 0,05 или не более 0,03 г/л, исходя в каждом случае из содержания накопленного в клетке глицина и/или в ферментационной среде после завершения ферментации.
В пределах рода Corynebacterium предпочтение отдают штаммам в соответствии с настоящим изобретением, которые относятся к следующим видам: Corynebacterium efficiens, такой как типовой штамм DSM44549, Corynebacterium glutamicum, такой как типовой штамм ATCC13032 или штамм R, Corynebacterium ammoniagenes, такой как типовой штамм ATCC6871, Corynebacterium humireducens, такой как типовой штамм DSM 45392, и Corynebacterium pekinese, такой как штамм CGMCC №5361.
Особое предпочтение отдают видам Corynebacterium glutamicum и Corynebacterium humireducens. В контексте данной заявки упоминают штамм Corynebacterium humireducens, при этом указанный штамм представляет собой предпочтительно штамм DSM 45392 или происходящий от него штамм.
Некоторые представители вида Corynebacterium glutamicum также известны из предшествующего уровня техники под другими названиями. Данные штаммы, например, включают: Corynebacterium acetoacidophilum ATCC13870, Corynebacterium lilium DSM20137, Corynebacterium melassecola ATCC17965, Brevibacterium flavum ATCC14067, Brevibacterium lactofermentum ATCC13869 и Brevibacterium divaricatum ATCC14020. Также для Corynebacterium glutamicum использовали эквивалентный термин «Micrococcus glutamicus». Некоторые представители вида Corynebacterium efficiens также были обозначены в предшествующем уровне техники как Corynebacterium thermoaminogenes, как, например, штамм FERM BP-1539.
Информацию в отношении таксономической классификации штаммов группы коринеформных бактерий можно найти, в частности, в Seiler (Journal of General Microbiology 129, 1433-1477 (1983)), Kinoshita (1985, Glutamic Acid Bacteria, p 115-142. В: Demain and Solomon (ed), Biology of Industrial Microorganisms. The Benjamin/Cummins Publishing Co., London, UK),
Figure 00000001
and Kroppenstedt (Canadian Journal of Microbiology 42, 989-1005 (1996)), Liebl et al (International Journal of Systematic Bacteriology 41, 255-260 (1991)), Fudou et al (International Journal of Systematic and Evolutionary Microbiology 52, 1127-1131 (2002)) и в US-A-5250434.
Штаммы с обозначение «ATCC» можно получить из Американской коллекции типовых культур (Манассас, Вайоминг, США). Штаммы с обозначением «DSM» можно получить из Немецкой коллекции микроорганизмов и клеточных культур (German Microorganism and Cell Culture Collection) (DSMZ, Брауншвейг, Германия). Штаммы с обозначением «NRRL» можно получить из Коллекции запатентованных культур службы сельскохозяйственных исследований (ARS, Пеория, Иллинойс, США). Штаммы с обозначением «FERM» можно получить из Национального института перспективных технических наук и технологий (AIST Tsukuba Central 6, 1-1-1 Higashi, Тсукуба Ибараки, Япония). Штаммы с обозначением «CGMCC» можно получить из Центральной коллекции культур основных микроорганизмов (CGMCC, Пекин, Китай).
Настоящее изобретение также дополнительно относится к способу получения в избыточном количестве L-аминокислоты, характеризующемуся тем, что в указанном способе используют по меньшей мере один фермент в соответствии с настоящим изобретением, и/или полинуклеотид, кодирующий указанный фермент, и/или систему расщепления глицина в соответствии с настоящим изобретением, и/или полинуклеотиды, кодирующие указанную систему, и/или рекомбинантный микроорганизм в соответствии с настоящим изобретением, предпочтительно рекомбинантную бактерию в соответствии с настоящим изобретением, в частности, рекомбинантную коринеформную бактерию в соответствии с настоящим изобретением, в особенности предпочтительно рекомбинантную коринебактерию в соответствии с настоящим изобретением, в особенности коринебактерию видов C.humireducens или C.glutamicum. В предпочтительном варианте осуществления в соответствии с настоящим изобретением по меньшей мере один полинуклеотид в соответствии с настоящим изобретением используют в данном случае в сверхэкспрессируемой форме.
Настоящее изобретение в данном случае предпочтительно относится к способу получения в избыточном количестве L-аминокислоты, характеризующемуся тем, что в указанном способе используют систему расщепления глицина, содержащую ферменты GcvP, GcvT и GcvH, причем система расщепления глицина содержит по меньшей мере один из ферментов GcvP, GcvT и GcvH в соответствии с настоящим изобретением, и/или в указанном способе используют полинуклеотиды, кодирующие ферменты GcvP, GcvT и GcvH системы расщепления глицина, при этом указанные полинуклеотиды содержат по меньшей мере один из полинуклеотидов gcvP, gcvT и gcvH в соответствии с настоящим изобретением, и/или используют рекомбинантную коринебактерию, предпочтительно видов C.humireducens или C.glutamicum, которая содержит по меньшей мере один фермент в соответствии с настоящим изобретением, предпочтительно все три фермента в соответствии с настоящим изобретением, выбранные из GcvP, GcvT и GcvH, и/или по меньшей мере один полинуклеотид в соответствии с настоящим изобретением, предпочтительно все три полинуклеотида в соответствии с настоящим изобретением, выбранные из gcvP, gcvT и gcvH.
Настоящее изобретение в данном случае особенно предпочтительно относится к способу получения в избыточном количестве L-аминокислоты, характеризующемуся тем, что в указанном способе используют систему расщепления глицина, которая содержит ферменты GcvP, GcvT и GcvH в соответствии с настоящим изобретением, и/или полинуклеотиды, кодирующие указанные ферменты, и/или в указанном способе используют рекомбинантную коринебактерию, предпочтительно видов C.humireducens или C.glutamicum, которая содержит такую систему расщепления глицина и/или полинуклеотиды, кодирующие указанную систему.
«L-аминокислоту» в соответствии с настоящим изобретением необходимо понимать как, в частности, протеиногенные L-аминокислоты.
В данном случае L-аминокислота предпочтительно выбрана из L-аланина, L-валина, L-аминокислот семейства глутамата, в частности L-глутамата, L-глутамина, L-пролина и L-аргинина, и L-аминокислот семейства аспартата, в частности L-аспартата, L-аспарагина, L-метионина, L-лизина, L-изолейцина и L-треонина, особенно предпочтительно выбрана из L-аланина, L-валина, L-глутамата, L-метионина, L-лизина и L-треонина. Особое предпочтение отдают L-метионину.
Получение в избыточном количестве L-аминокислот предпочтительно осуществляет с помощью C.humireducens или C.glutamicum.
Способы в соответствии с настоящим изобретением предпочтительно характеризуются тем, что образуются лишь незначительные количества побочного продукта, представляющего собой глицин. В способе в соответствии с настоящим изобретением глицин образуется предпочтительно в количестве менее 0,2 г/л, в частности в количестве менее 0,1 г/л, особенно предпочтительно в количестве менее 0,05 г/л.
«Продуцировать(получать) в избыточном количестве» или «продуцирование(получение) в избыточном количестве» по отношению к L-аминокислотам в соответствии с настоящим изобретением необходимо понимать в том значении, что микроорганизмы продуцируют L-аминокислоты в соответствии с их собственными потребностями, при этом аминокислоты либо концентрируются в клетке, либо секретируются в окружающую питательную среду, где они накапливаются. В данном случае микроорганизмы предпочтительно характеризуются способностью к концентрированию или накоплению в клетке или в питательной среде ≥ (по меньшей мере) 0,25 г/л, ≥0,5 г/л, ≥1,0 г/л, ≥1,5 г/л, ≥2,0 г/л, ≥4 г/л или ≥10 г/л соответствующих L-аминокислот в течение ≤ (не более) 120 часов, ≤96 часов, ≤48 часов, ≤36 часов, ≤24 часов или ≤12 часов.
В соответствии с настоящим изобретением рекомбинантные микроорганизмы, в которые были встроены полинуклеотиды в соответствии с настоящим изобретением и/или векторы в соответствии с настоящим изобретением, уже в предпочтительном варианте осуществления имеют способность к продуцированию в избыточном количестве L-аминокислоты перед встраиванием в них полинуклеотидов и/или векторов в соответствии с настоящим изобретением. Исходные штаммы предпочтительно представляют собой штаммы, которые были получены путем мутагенеза и селекции, с помощью методик рекомбинантной ДНК или путем комбинации обоих методик.
Является очевидным и не требует дополнительного пояснения тот факт, что рекомбинантный микроорганизм в соответствии с настоящим изобретением также может быть получен таким способом, при котором в диком штамме, в котором присутствует или был встроен полинуклеотид в соответствии с настоящим изобретением и/или вектор в соответствии с настоящим изобретением, и посредством дополнительных подходящих методик генной инженерии, что вызывает продуцирование L-аминокислоты или повышение продуцирования L-аминокислоты.
Настоящее изобретение также дополнительно относится к другим полинуклеотидам из C.humireducens, а также к полипептидам, кодируемым указанными полинуклеотидами. Сверхэкспрессия соответствующих полинуклеотидов или полипептидов может оказывать положительное воздействие на продуцирование определенных L-аминокислот.
Таким образом, настоящее изобретение также относится к
a) треониндегидратазе (IlvA, EC 4.3.1.19) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 106, и кодирующим ее полинуклеотидам,
b) субъединице ацетолактатсинтазы (IIvB) которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 98, и кодирующим ее полинуклеотидам,
c) изомероредуктазе (IlvC, EC 1.1.1.86) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 100, и кодирующим ее полинуклеотидам,
d) дегидратазе дигидроксикислот (IlvD, EC 4.2.1.9) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 102, и кодирующим ее полинуклеотидам,
e) трансаминазе (IlvE, EC 2.6.1.42) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 104, и кодирующим ее полинуклеотидам,
f) ацетолактатсинтазе (IlvH, EC 2.2.1.6) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 122, и кодирующим ее полинуклеотидам,
g) 3-метил-2-оксобутаноат-гидроксиметилтрансферазе (PanB, EC 2.1.2.11) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 118, и кодирующим ее полинуклеотидам,
h) пантотенатсинтазе (PanC, EC 6.3.2.1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 120, и кодирующим ее полинуклеотидам,
i) глутаматдегидрогеназе (Gdh) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 124, и кодирующим ее полинуклеотидам,
j) глутаминсинтетазе (глутаминсинтетазе 1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 126, и кодирующим ее полинуклеотидам,
k) глутаминсинтетазе (глутаминсинтетазе 2) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 128, и кодирующим ее полинуклеотидам,
l) глутаматсинтазе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 130, и кодирующим ее полинуклеотидам,
m) изоцитратдегидрогеназе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 132, и кодирующим ее полинуклеотидам,
n) аконитатгидразе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 134, и кодирующим ее полинуклеотидам,
o) цитратсинтазе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 136, и кодирующим ее полинуклеотидам,
p) аминопептидазе С (PepC)с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 138, и кодирующим ее полинуклеотидам,
q) пируватдегидрогеназе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 140, и кодирующим ее полинуклеотидам,
r) пируваткиназе (пируваткиназе 1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 142, и кодирующим ее полинуклеотидам,
s) пируваткиназе (пируваткиназе 2) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 144, и кодирующим ее полинуклеотидам,
t) енолазе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 146, и кодирующим ее полинуклеотидам,
u) 2,3-Бисфосфоглицерат-зависимой фосфоглицератмутазе (GpmA) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 148, и кодирующим ее полинуклеотидам,
v) фосфоглицераткиназе (Pgk) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 150, и кодирующим ее полинуклеотидам,
w) глицеральдегид-3-фосфат-дегидрогеназе (глицерин-3-фосфат-дегидрогеназе 1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 152, и кодирующим ее полинуклеотидам,
x) глицеральдегид-3-фосфат-дегидрогеназе (глицерин-3-фосфат-дегидрогеназе 2) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 154, и кодирующим ее полинуклеотидам,
y) триозофосфатизомеразе (TpiA) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 156, и кодирующим ее полинуклеотидам,
z) фруктозобисфосфатальдолазе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 158, и кодирующим ее полинуклеотидам,
aa) 1-фосфофруктокиназе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 160, и кодирующим ее полинуклеотидам,
bb) 6-фосфофруктокиназе с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 162, и кодирующим ее полинуклеотидам,
cc) гомосеринкиназе (ThrB, EC 2.7.1.39) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 4, и кодирующим ее полинуклеотидам,
dd) цистеинсинтазе (CBS, CysK) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 22, и кодирующим ее полинуклеотидам,
ee) цистатионин-бета-лиазе (AecD) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 26, и кодирующим ее полинуклеотидам,
ff) аспартатполуальдегиддегидрогеназе (Asd, EC 1.2.1.11) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 28, и кодирующим ее полинуклеотидам,
gg) малой субъединице транспортера аминокислот с разветвленной цепью (BrnE) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 30, и кодирующим ее полинуклеотидам,
hh) большой субъединице транспортера аминокислот с разветвленной цепью (BrnF) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 32, и кодирующим ее полинуклеотидам,
ii) серинацетилтрансферазе (CysE) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 34, и кодирующим ее полинуклеотидам,
jj) цистеинсинтазе (CysK) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 36, и кодирующим ее полинуклеотидам,
kk) серингидроксиметилтрансферазе (GlyA) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 44, и кодирующим ее полинуклеотидам,
ll) необязательно устойчивой к ингибированию конечным продуктом гомосериндегидрогеназе (Hom, EC 1.2.1.11) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 46, и кодирующим ее полинуклеотидам,
mm) необязательно устойчивой к ингибированию конечным продуктом аспартаткиназе (LysC, EC 2.7.2.4) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 54, и кодирующим ее полинуклеотидам,
nn) цистатионин-гамма-синтазе (MetB) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 56, и кодирующим ее полинуклеотидам,
oo) 5,10-метилентетрагидрофолатредуктазе (MetF) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 58, и кодирующим ее полинуклеотидам,
pp) гомосерин-О-ацетилтрансферазе (MetX) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 60, и кодирующим ее полинуклеотидам,
qq) О-ацетилгомосеринлиазе (MetY) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 62, и кодирующим ее полинуклеотидам,
rr) необязательно устойчивой к ингибированию конечным продуктом пируваткарбоксилазе (Pyc, EC 6.4.1.1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 64, и кодирующим ее полинуклеотидам,
ss) необязательно устойчивой к ингибированию конечным продуктом D-3-фосфоглицератдегидрогеназе (SerA) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 66, и кодирующим ее полинуклеотидам,
tt) фосфосеринфосфатазе (SerB) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 68, и кодирующим ее полинуклеотидам,
uu) фосфосеринаминотрансферазе (SerC) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 70, и кодирующим ее полинуклеотидам,
vv) субъединице сульфатаденилилтрансферазы (CysD), которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 74, и кодирующим ее полинуклеотидам,
ww) аденозинфосфосульфатредуктазе (CysH) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 76, и кодирующим ее полинуклеотидам,
xx) сульфитредуктазе (CysI) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 78, и кодирующим ее полинуклеотидам,
yy) бета-цепи НАДФ-зависимой глутаматсинтазы (CysJ) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 80, и кодирующим ее полинуклеотидам,
zz) большой субъединице сульфатаденилилтрансферазы (CysN), которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 82, и кодирующим ее полинуклеотидам,
aaa) цистатионин-бета-синтазе (CysY) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 84, и кодирующим ее полинуклеотидам,
bbb) транспортеру сульфата (CysZ) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 86, и кодирующим его полинуклеотидам,
ccc) 5-метилтетрагидроптероилтриглутаматгомоцистеинметилтрансферазе (MetE) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 88, и кодирующим ее полинуклеотидам,
ddd) пептидил-tRNA-гидролазе 1 (PtH1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 90, и кодирующим ее полинуклеотидам,
eee) пептидил-tRNA-гидролазе 2 (PtH2) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 92, и кодирующим ее полинуклеотидам,
fff) диаминопимелатдегидрогеназе (Ddh, EC 1.4.1.16) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 202, и кодирующим ее полинуклеотидам,
ggg) диаминопимелатдекарбоксилазе (LysA, EC 4.1.1.20) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 164, и кодирующим ее полинуклеотидам,
hhh) аспартатаминотрансферазе (AaT, EC 2.6.1.1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 166, и кодирующим ее полинуклеотидам,
iii) экспортеру L-лизина (LysE, пермеазе эффлюкса лизина) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 168, и кодирующим его полинуклеотидам,
jjj) дигидропиколинатредуктазе (DapB, EC 1.3.1.26) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 170, и кодирующим ее полинуклеотидам,
kkk) глюкозо-6-фосфат-дегидрогеназе (EC 1.1.1.49) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 172, и кодирующим ее полинуклеотидам,
lll) субъединице Zwf глюкозо-6-фосфат-дегидрогеназы (Zwf, EC 1.1.1.49) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 186, и кодирующим ее полинуклеотидам,
mmm) субъединице OpcA глюкозо-6-фосфат-дегидрогеназы (OpcA, EC 1.1.1.49) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 188, и кодирующим ее полинуклеотидам,
nnn) дегидрогеназе фосфоглюконовой кислоты (Gnd, EC 1.1.1.44) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 174, и кодирующим ее полинуклеотидам.
Настоящее изобретение также дополнительно относится к векторам, содержащим упомянутые выше полинуклеотиды, а также к рекомбинантным микроорганизмам, содержащим упомянутые выше ферменты, и/или полинуклеотиды, и/или векторы. В предпочтительном варианте осуществления соответствующий полипептид и/или полинуклеотид в данном случае присутствует в микроорганизме в сверхэкспрессируемой форме. Рекомбинантные микроорганизмы в данном случае предпочтительно являются коринеформными бактериями, в частности коринебактериями, в особенности коринебактериями видов C.humireducens или C.glutamicum.
Таким образом, настоящее изобретение также дополнительно относится к способу получения в избыточном количестве L-аминокислоты, предпочтительно выбранной из L-аланина, L-валина, L-аминокислот семейства глутамата, в частности L-глутамата, L-глутамина, L-пролина и L-аргинина, и из L-аминокислот семейства аспартата, в частности L-аспартата, L-аспарагина, L-метионина, L-лизина, L-изолейцина и L-треонина, особенно предпочтительно выбранной из L-аланина, L-валина, L-глутамата, L-метионина, L-лизина и L-треонина, в частности для получения в избыточном количестве L-метионина, в котором по меньшей мере один, предпочтительно по меньшей мере два, три или четыре упомянутых полинуклеотида присутствуют в сверхэкспрессируемой форме, при этом способ предпочтительно осуществляют с использованием коринебактерий, в особенности коринебактерий видов C.humireducens или C.glutamicum.
Настоящее изобретение также дополнительно относится к другим полинуклеотидам из C.humireducens, а также к полипептидам, кодируемым указанными полинуклеотидами. Инактивация или аттенуация соответствующих полинуклеотидов или полипептидов может оказывать положительное воздействие на продуцирование определенных L-аминокислот.
Таким образом, настоящее изобретение также относится к полипептидам, выбранным из следующего списка:
a) треонинсинтаза (ThrC, EC 4.2.3.1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 108, и кодирующие ее полинуклеотиды,
b) изопропилмалатсинтаза (LeuA, EC 2.3.3.13) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 110, и кодирующие ее полинуклеотиды,
c) изопропилмалатдегидрогеназа (LeuB, EC 1.1.1.85) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 112, и кодирующие ее полинуклеотиды,
d) субъединицы изопропилмалатизомеразы (LeuCD, EC 4.2.1.33) с последовательностями, которые по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентичны последовательностям под SEQ ID NO: 114 или SEQ ID NO: 116, и кодирующие их полинуклеотиды,
e) субъединицы сукцинил-CoA-лигазы (SucCD, EC 6.2.1.5) с последовательностями, которые по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентичны последовательностям под SEQ ID NO: 198 или SEQ ID NO: 200, и кодирующие их полинуклеотиды,
f) ДНК-связывающий домен HTH tetR-типа (McbR) с последовательностью, которая по меньшей мере на, 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 2, и кодирующие его полинуклеотиды,
g) гомосеринкиназа (ThrB, EC 2.7.1.39) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 4, и кодирующие ее полинуклеотиды,
h) глюкозо-6-фосфат-изомераза (Pgi, EC 5.3.1.9) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 6, и кодирующие ее полинуклеотиды,
i) фосфоенолпируваткарбоксикиназа (Pck, EC 4.1.1.32) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 8, и кодирующие ее полинуклеотиды,
j) D-метионин-связывающий липопротеин (MetQ) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 10, и кодирующие его полинуклеотиды,
k) транспортер метионина (MetP) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 12, и кодирующие его полинуклеотиды,
l) АТФ-зависимый транспортер метионина (MetN) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 14, и кодирующие его полинуклеотиды,
m) S-аденозилметионинсинтаза (MetK) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 16, и кодирующие ее полинуклеотиды,
n) пермеаза системы импорта метионина (MetI) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 18, и кодирующие ее полинуклеотиды,
o) 4-гидрокси-тетрагидродипиколинатсинтаза (DapA, EC 4.3.3.7) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 20, и кодирующие ее полинуклеотиды,
p) карбоксилатаминлигаза с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 24, и кодирующие ее полинуклеотиды,
q) малат:хинон-оксидоредуктаза (Mqo, EC 1.1.99.16) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 176, и кодирующие ее полинуклеотиды,
r) субъединица Е1р пируватдегидрогеназного комплекса (AceE, EC 1.2.4.1) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 178, и кодирующие ее полинуклеотиды,
s) цитратсинтаза (GltA, EC 4.1.3.7) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 180, и кодирующие ее полинуклеотиды,
t) малатдегидрогеназа (Mdh, EC 1.1.1.37) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 182, и кодирующие ее полинуклеотиды,
u) UDP-N-ацетилмурамоилаланил-D-глутамат-2,6-диаминопимелатлигаза (MurE, EC 6.3.2.13) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 184, и кодирующие ее полинуклеотиды.
Настоящее изобретение также дополнительно относится к векторам, содержащим упомянутые выше полинуклеотиды, а также к рекомбинантным микроорганизмам, содержащим упомянутые выше ферменты, и/или полинуклеотиды, и/или векторы. В предпочтительном варианте осуществления соответствующий полипептид и/или полинуклеотид в данном случае присутствует в микроорганизме в инактивированной или аттенуированной форме. Рекомбинантные микроорганизмы в данном случае предпочтительно являются коринеформными бактериями, в частности коринебактериями, в особенности коринебактериями видов C.humireducens или C.glutamicum, в особенности вида C.humireducens.
Таким образом, настоящее изобретение также дополнительно относится к способу получения в избыточном количестве L-аминокислоты, предпочтительно выбранной из L-аланина, L-валина, L-аминокислот семейства глутамата, в частности L-глутамата, L-глутамина, L-пролина и L-аргинина, и из L-аминокислот семейства аспартата, в частности L-аспартата, L-аспарагина, L-метионина, L-лизина, L-изолейцина и L-треонина, особенно предпочтительно выбранной из L-аланина, L-валина, L-глутамата, L-метионина, L-лизина и L-треонина, в частности для получения в избыточном количестве L-метионина, в котором по меньшей мере один, предпочтительно по меньшей мере два, три или четыре упомянутых полинуклеотида присутствуют в инактивированной или аттенуированной форме, при этом способ предпочтительно осуществляют с использованием коринебактерий, в особенности коринебактерий видов C.humireducens или C.glutamicum. Таким образом, в предпочтительном варианте осуществления по меньшей мере один, предпочтительно по меньшей мере два, три или четыре полинуклеотида из упомянутых в подробном списке выше в данном случае присутствуют в сверхэкспрессируемой форме.
В дополнительном предпочтительном варианте осуществления в соответствии с настоящим изобретением микроорганизмы или бактерии в соответствии с настоящим изобретением, в частности коринебактерии в соответствии с настоящим изобретением, в особенности коринебактерии в соответствии с настоящим изобретением видов C.humireducens или C.glutamicum, в частности штаммы, продуцирующие в избыточном количестве L-метионин в соответствии с настоящим изобретением, в дополнение к заявляемой, предпочтительно сверхэкспрессируемой системе расщепления глицина или полинуклеотидам, кодирующим указанную систему, имеют по меньшей мере один, предпочтительно по меньшей мере два или три, в частности предпочтительно по меньшей мере четыре или пять из следующих компонентов:
a) аттенуированный полинуклеотид (mcbR), который кодирует ДНК-связывающий домен HTH tetR-типа (McbR), предпочтительно ДНК-связывающий домен с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 2,
b) аттенуированный полинуклеотид (ген thrB), который кодирует гомосеринкиназу (ThrB, EC 2.7.1.39), предпочтительно гомосеринкиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 4,
c) аттенуированный полинуклеотид (pgi), который кодирует глюкозо-6-фосфат-изомеразу (Pgi, EC 5.3.1.9), предпочтительно глюкозо-6-фосфат-изомеразу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 6,
d) аттенуированный полинуклеотид (pck), который кодирует фосфоенолпируваткарбоксикиназу (Pck, EC 4.1.1.32), предпочтительно фосфоенолпируваткарбоксикиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 8,
e) аттенуированный полинуклеотид (metQ), который кодирует D-метионин-связывающий липопротеин (MetQ), предпочтительно D-метионин-связывающий липопротеин с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 10,
f) аттенуированный полинуклеотид (metP), который кодирует транспортер метионина (MetP), предпочтительно транспортер метионина с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 12,
g) аттенуированный полинуклеотид (metN), который кодирует АТФ-зависимый транспортер метионина (MetN), предпочтительно АТФ-зависимый транспортер метионина с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 14,
h) аттенуированный полинуклеотид (metK), который кодирует S-аденозилметионинсинтазу (MetК), предпочтительно S-аденозилметионинсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 16,
i) аттенуированный полинуклеотид (metI), который кодирует пермеазу системы импорта метионина (MetI), предпочтительно пермеазу системы импорта метионина с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 18,
j) аттенуированный полинуклеотид (dapA), который кодирует 4-гидрокси-тетрагидродипиколинатсинтазу (DapA), предпочтительно 4-гидрокси-тетрагидродипиколинатсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 20,
k) сверхэкспрессируемый полинуклеотид (CBS), который кодирует цистеинсинтазу (CBS), предпочтительно цистеинсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 22,
l) аттенуированный полинуклеотид, который кодирует гомолог cg3031, предпочтительно гомолог cg3031 с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 24,
m) сверхэкспрессируемый полинуклеотид (aecD), который кодирует цистатионин-бета-лиазу (AecD), предпочтительно цистатионин-бета-лиазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 26,
n) сверхэкспрессируемый полинуклеотид (asd), который кодирует аспартатполуальдегиддегидрогеназу (Asd), предпочтительно аспартатполуальдегиддегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 28,
o) сверхэкспрессируемый полинуклеотид (metH), который кодирует 5-метилтетрагидрофолатгомоцистеинметилтрансферазу (MetH, EC 2.1.1.13),
p) сверхэкспрессируемый полинуклеотид (brnE), который кодирует малую субъединицу транспортера аминокислот с разветвленной цепью (BrnE), предпочтительно субъединицу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 30,
q) сверхэкспрессируемый полинуклеотид (brnF), который кодирует большую субъединицу транспортера аминокислот с разветвленной цепью (BrnF), предпочтительно субъединицу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 32,
r) сверхэкспрессируемый полинуклеотид (cysE), который кодирует серинацетилтрансферазу (CysE), предпочтительно серинацетилтрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 34,
s) сверхэкспрессируемый полинуклеотид (cysK), который кодирует цистеинсинтазу (CysK), предпочтительно цистеинсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 36,
t) сверхэкспрессируемый полинуклеотид (glyA), который кодирует серингидроксиметилтрансферазу (GlyA), предпочтительно серингидроксиметилтрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 44,
u) сверхэкспрессируемый полинуклеотид (hom), который кодирует необязательно устойчивую к ингибированию конечным продуктом гомосериндегидрогеназу (Hom), предпочтительно гомосериндегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 46,
v) сверхэкспрессируемый полинуклеотид (lysC), который кодирует необязательно устойчивую к ингибированию конечным продуктом аспартаткиназу (LysC), предпочтительно аспартаткиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 54,
w) сверхэкспрессируемый полинуклеотид (metB), который кодирует цистатионин-гамма-синтазу (MetB), предпочтительно цистатионин-гамма-синтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 56,
x) сверхэкспрессируемый полинуклеотид (metF), который кодирует 5,10-метилентетрагидрофолатредуктазу (MetF), предпочтительно 5,10-метилентетрагидрофолатредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 58,
y) сверхэкспрессируемый полинуклеотид (metX), который кодирует гомосерин-О-ацетилтрансферазу (MetX), предпочтительно гомосерин-О-ацетилтрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 60,
z) сверхэкспрессируемый полинуклеотид (metY), который кодирует O-ацетилгомосеринлиазу (MetY), предпочтительно O-ацетилгомосеринлиазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 62,
aa) сверхэкспрессируемый полинуклеотид (pyc), который кодирует пируваткарбоксилазу (Рyc), предпочтительно пируваткарбоксилазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 64,
bb) сверхэкспрессируемый полинуклеотид (serA), который кодирует необязательно устойчивую к ингибированию конечным продуктом D-3-фосфоглицератдегидрогеназу (SerA), предпочтительно D-3-фосфоглицератдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 66,
cc) сверхэкспрессируемый полинуклеотид (serB), который кодирует фосфосеринфосфатазу (serB), предпочтительно фосфосеринфосфатазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 68,
dd) сверхэкспрессируемый полинуклеотид (serC), который кодирует фосфосеринаминотрансферазу (SerC), предпочтительно фосфосеринаминотрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 70,
ee) сверхэкспрессируемый полинуклеотид (ald), который кодирует аланиндегидрогеназу (Ald), предпочтительно аланиндегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 72,
ff) сверхэкспрессируемый полинуклеотид (cysD), который кодирует субъединицу сульфатаденилилтрансферазы (CysD), предпочтительно субъединицу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 74,
gg) сверхэкспрессируемый полинуклеотид (cysH), который кодирует аденозинфосфосульфатредуктазу (CysH), предпочтительно аденозинфосфосульфатредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 76,
hh) сверхэкспрессируемый полинуклеотид (cysI), который кодирует сульфитредуктазу (CysI), предпочтительно сульфитредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 78,
ii) сверхэкспрессируемый полинуклеотид (cysJ), который кодирует (CysJ), предпочтительно (CysJ) с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 80,
jj) сверхэкспрессируемый полинуклеотид (cysN), который кодирует субъединицу сульфатаденилилтрансферазы (CysN), предпочтительно субъединицу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 82,
kk) сверхэкспрессируемый полинуклеотид (cysY), который кодирует цистатионин-бета-синтазу (CysY), предпочтительно цистатионин-бета-синтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 84,
ll) сверхэкспрессируемый полинуклеотид (cysZ), который кодирует предполагаемый транспортер сульфата (CysZ), предпочтительно транспортер сульфата с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 86,
mm) сверхэкспрессируемый полинуклеотид (metE), который кодирует 5-метилтетрагидроптероилтриглутаматгомоцистеинметилтрансферазу (MetE), предпочтительно белок с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 88,
nn) сверхэкспрессируемый полинуклеотид (ptH1), который кодирует пептидил-tRNA-гидролазу 1 (PtH1), предпочтительно пептидил-tRNA-гидролазу 1 с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 90,
oo) сверхэкспрессируемый полинуклеотид (ptH2), который кодирует пептидил-tRNA-гидролазу 2 (PtH2), предпочтительно пептидил-tRNA-гидролазу 2 с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 92.
Настоящее изобретение также дополнительно относится к соответствующему способу получения в избыточном количестве L-аминокислоты, в частности L-метионина, в котором используют такой микроорганизм или такую бактерию.
В дополнительном предпочтительном варианте осуществления в соответствии с настоящим изобретением микроорганизмы или бактерии в соответствии с настоящим изобретением, в частности коринебактерии в соответствии с настоящим изобретением, в особенности коринебактерии в соответствии с настоящим изобретением видов C.humireducens или C.glutamicum, в частности штаммы, продуцирующие в избыточном количестве L-валин, в соответствии с настоящим изобретением, в дополнение к заявляемой, предпочтительно сверхэкспрессируемой системе расщепления глицина или полинуклеотидам, кодирующим указанную систему, имеют по меньшей мере один, предпочтительно по меньшей мере 2 или 3, в частности предпочтительно по меньшей мере 4 или 5 из следующих компонентов:
a) сверхэкспрессируемый полинуклеотид (ген ilvA), который кодирует треониндегидратазу (IIvA EC 4.3.1.19), предпочтительно треониндегидратазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 106,
b) сверхэкспрессируемый полинуклеотид (ген ilvB), который кодирует субъединицу ацетолактатсинтазы (IIvB), предпочтительно субъединицу ацетолактатсинтазы с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 98,
c) сверхэкспрессируемый полинуклеотид (ген ilvN), который кодирует необязательно устойчивую к ингибированию конечным продуктом субъединицу ацетолактатсинтазы (IlvN),
d) сверхэкспрессируемый полинуклеотид (ген ilvC), который кодирует изомероредуктазу (IIvC, EC 1.1.1.86), предпочтительно изомероредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 100,
e) сверхэкспрессируемый полинуклеотид (ген ilvD), который кодирует дегидратазу дигидроксикислот (IIvD, EC 4.2.1.9), предпочтительно дегидратазу дигидроксикислот с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 102,
f) сверхэкспрессируемый полинуклеотид (ген ilvE), который кодирует трансаминазу (IIvE, EC 2.6.1.42), предпочтительно трансаминазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 104,
g) сверхэкспрессируемый полинуклеотид (ген ilvH), который кодирует ацетолактатсинтазу (IIvH, EC 2.2.1.6), предпочтительно ацетолактатсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 122,
h) аттенуированный полинуклеотид (ген thrB), который кодирует гомосеринкиназу (ThrB, EC 2.7.1.39), предпочтительно гомосеринкиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 4,
i) аттенуированный полинуклеотид (ген thrC), который кодирует треонинсинтазу (ThrC, EC 4.2.3.1), предпочтительно треонинсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 108,
j) сверхэкспрессируемый полинуклеотид (ген hom), который кодирует необязательно устойчивую к ингибированию конечным продуктом гомосериндегидрогеназу (Hom, EC 1.2.1.11), предпочтительно гомосериндегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 46,
k) аттенуированный полинуклеотид (ген leuA), который кодирует необязательно устойчивую к ингибированию конечным продуктом изопропилмалатсинтазу (LeuA, EC 2.3.3.13), предпочтительно изопропилмалатсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 110,
l) аттенуированный полинуклеотид (ген leuB), который кодирует изопропилмалатдегидрогеназу (LeuB, EC 1.1.1.85), предпочтительно изопропилмалатдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 112,
m) аттенуированные полинуклеотиды (ген leuCD), которые кодируют субъединицы изопропилмалатизомеразы (LeuCD, EC 4.2.1.33), предпочтительно субъединицы изопропилмалатизомеразы с последовательностями, которые по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентичны последовательностям под SEQ ID NO: 114 и SEQ ID NO: 116,
n) сверхэкспрессируемый полинуклеотид (ген panB), который кодирует 3-метил-2-оксобутаноат-гидроксиметилтрансферазу (PanB, EC 2.1.2.11), предпочтительно 3-метил-2-оксобутаноат-гидроксиметилтрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 118,
o) сверхэкспрессируемый полинуклеотид (ген panC), который кодирует пантотенатсинтазу (PanC, EC 6.3.2.1), предпочтительно пантотенатсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 120.
Настоящее изобретение также дополнительно соответственно относится к способу получения в избыточном количестве L-аминокислоты, в частности L-валина, в котором используют такой микроорганизм или такую бактерию.
В дополнительном предпочтительном варианте осуществления в соответствии с настоящим изобретением микроорганизмы или бактерии в соответствии с настоящим изобретением, в частности коринебактерии в соответствии с настоящим изобретением, в особенности коринебактерии в соответствии с настоящим изобретением видов C.humireducens или C.glutamicum, в частности штаммы, продуцирующие в избыточном количестве L-глутамат, в соответствии с настоящим изобретением, в дополнение к заявляемой, предпочтительно сверхэкспрессируемой системе расщепления глицина или полинуклеотидам, кодирующим указанную систему, имеют по меньшей мере один, предпочтительно по меньшей мере два или три, в частности предпочтительно по меньшей мере четыре или пять из следующих компонентов:
a) сверхэкспрессируемый полинуклеотид (gdh), который кодирует глутаматдегидрогеназу (Gdh), предпочтительно глутаматдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 124,
b) сверхэкспрессируемый полинуклеотид, который кодирует глутаминсинтетазу (глутаминсинтетазу 1), предпочтительно глутаминсинтетазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 126,
c) сверхэкспрессируемый полинуклеотид, который кодирует глутаминсинтетазу (глутаминсинтетазу 2), предпочтительно глутаминсинтетазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 128,
d) сверхэкспрессируемый полинуклеотид, который кодирует глутаматсинтазу, предпочтительно глутаматсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 130,
e) сверхэкспрессируемый полинуклеотид, который кодирует изоцитратдегидрогеназу, предпочтительно изоцитратдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 132,
f) сверхэкспрессируемый полинуклеотид, который кодирует аконитатгидразу, предпочтительно аконитатгидразу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 134,
g) сверхэкспрессируемый полинуклеотид, который кодирует цитратсинтазу, предпочтительно цитратсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 136,
h) сверхэкспрессируемый полинуклеотид (pepC), который кодирует аминопептидазу C (PepC), предпочтительно аминопептидазу C с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 138,
i) сверхэкспрессируемый полинуклеотид, который кодирует пируватдегидрогеназу, предпочтительно пируватдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 140,
j) сверхэкспрессируемый полинуклеотид, который кодирует пируваткиназу (пируваткиназу 1), предпочтительно пируваткиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 142,
k) сверхэкспрессируемый полинуклеотид, который кодирует пируваткиназу (пируваткиназу 2), предпочтительно пируваткиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 144,
l) сверхэкспрессируемый полинуклеотид, который кодирует енолазу, предпочтительно енолазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 146,
m) сверхэкспрессируемый полинуклеотид (gpmA), который кодирует 2,3-бисфосфоглицерат-зависимую фосфоглицератмутазу (GpmA), предпочтительно фосфоглицератмутазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 148,
n) сверхэкспрессируемый полинуклеотид (pgk), который кодирует фосфоглицераткиназу (Pgk), предпочтительно фосфоглицераткиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 150,
o) сверхэкспрессируемый полинуклеотид, который кодирует глицеральдегид-3-фосфат-дегидрогеназу (глицеральдегид-3-фосфат-дегидрогеназу 1), предпочтительно глицеральдегид-3-фосфат-дегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 152,
p) сверхэкспрессируемый полинуклеотид, который кодирует глицеральдегид-3-фосфат-дегидрогеназу (глицеральдегид-3-фосфат-дегидрогеназу 2), предпочтительно глицеральдегид-3-фосфат-дегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 154,
q) сверхэкспрессируемый полинуклеотид (tpiA), который кодирует триозофосфатизомеразу (TpiA), предпочтительно триозофосфатизомеразу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 156,
r) сверхэкспрессируемый полинуклеотид, который кодирует фруктозобисфосфатальдолазу, предпочтительно фруктозобисфосфатальдолазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 158,
s) сверхэкспрессируемый полинуклеотид, который кодирует 1-фосфофруктокиназу, предпочтительно 1-фосфофруктокиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 160,
t) сверхэкспрессируемый полинуклеотид, который кодирует 6-фосфофруктокиназу, предпочтительно 6-фосфофруктокиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 162,
u) сверхэкспрессируемый полинуклеотид (pgi), который кодирует глюкозо-6-фосфат-изомеразу, предпочтительно глюкозо-6-фосфат-изомеразу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 6,
v) аттенуированные полинуклеотиды (sucCD), которые кодируют субъединицы сукцинил-CoA-лигазы (SucCD, EC 6.2.1.5), предпочтительно субъединицы сукцинил-CoA-лигазы с последовательностями, которые по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентичны последовательностям под SEQ ID NO: 198 или SEQ ID NO: 200.
Настоящее изобретение также дополнительно соответственно относится к способу получения в избыточном количестве L-аминокислоты, в частности L-глутамата, в котором используют такой микроорганизм или такую бактерию.
В дополнительном предпочтительном варианте осуществления в соответствии с настоящим изобретением микроорганизмы или бактерии в соответствии с настоящим изобретением, в частности коринебактерии в соответствии с настоящим изобретением, в особенности коринебактерии в соответствии с настоящим изобретением видов C.humireducens или C.glutamicum, в частности штаммы, продуцирующие в избыточном количестве L-аланин, в соответствии с настоящим изобретением, в дополнение к заявляемой, предпочтительно сверхэкспрессируемой системе расщепления глицина или полинуклеотидам, кодирующим указанную систему, имеют по меньшей мере один, предпочтительно по меньшей мере два или три, в частности предпочтительно по меньшей мере четыре или пять из следующих компонентов:
a) сверхэкспрессируемый полинуклеотид (alaD), который кодирует аланиндегидрогеназу (AlaD), предпочтительно аланиндегидрогеназу коринебактерий,
b) сверхэкспрессируемый полинуклеотид (gapA), который кодирует глицеральдегид-3-фосфат-дегидрогеназу (GapA), предпочтительно глицеральдегид-3-фосфат-дегидрогеназу коринебактерий,
c) инактивированный или аттенуированный полинуклеотид (IdhA), который кодирует L-лактатдегидрогеназу (LdhA), предпочтительно L-лактатдегидрогеназу коринебактерий,
d) инактивированный или аттенуированный полинуклеотид (ppc), который кодирует фосфоенолпируваткарбоксилазу (Ppc), предпочтительно фосфоенолпируваткарбоксилазу коринебактерий,
e) инактивированный или аттенуированный полинуклеотид (alr), который кодирует аланинрацемазу (Alr), предпочтительно аланинрацемазу коринебактерий.
Настоящее изобретение также дополнительно соответственно относится к способу получения в избыточном количестве L-аминокислоты, в частности L-аланина, в котором используют такой микроорганизм или такую бактерию.
В дополнительном предпочтительном варианте осуществления в соответствии с настоящим изобретением микроорганизмы или бактерии в соответствии с настоящим изобретением, в частности коринебактерии в соответствии с настоящим изобретением, в особенности коринебактерии в соответствии с настоящим изобретением видов C.humireducens или C.glutamicum, в частности штаммы, продуцирующие в избыточном количестве L-лизин, в соответствии с настоящим изобретением, в дополнение к заявляемой, предпочтительно сверхэкспрессируемой системе расщепления глицина или полинуклеотидам, кодирующим указанную систему, имеют по меньшей мере один, предпочтительно по меньшей мере 2 или 3, в частности предпочтительно по меньшей мере 4 или 5 из следующих компонентов:
a) сверхэкспрессируемый полинуклеотид (ген dapA), который кодирует дигидродипиколинатсинтазу (DapA, EC 4.2.1.52), предпочтительно дигидродипиколинатсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 20,
b) сверхэкспрессируемый полинуклеотид (lysC), который кодирует предпочтительно устойчивую к ингибированию конечным продуктом аспартаткиназу (LysC, EC 2.7.2.4), предпочтительно аспартаткиназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 54,
c) сверхэкспрессируемый полинуклеотид (ddh), который кодирует диаминопимелатдегидрогеназу (Ddh, EC 1.4.1.16), предпочтительно диаминопимелатдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 202,
d) сверхэкспрессируемый полинуклеотид (ген asd), который кодирует аспартатполуальдегиддегидрогеназу (Asd, EC 1.2.1.11), предпочтительно аспартатполуальдегиддегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 28,
e) сверхэкспрессируемый полинуклеотид (ген lysA), который кодирует диаминопимелатдекарбоксилазу (LysA, EC 4.1.1.20), предпочтительно диаминопимелатдекарбоксилазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 164,
f) сверхэкспрессируемый полинуклеотид (ген aat), который кодирует аспартатаминотрансферазу (AaT, EC 2.6.1.1), предпочтительно аспартатаминотрансферазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 166,
g) сверхэкспрессируемый полинуклеотид (ген lysE), который кодирует экспортер L-лизина (LysE, пермеазу эффлюкса лизина), предпочтительно экспортер L-лизина с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 168,
h) сверхэкспрессируемый полинуклеотид (ген pyc), который кодирует пируваткарбоксилазу (Pyc, EC 6.4.1.1), предпочтительно пируваткарбоксилазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 64,
i) сверхэкспрессируемый полинуклеотид (ген dapF), который кодирует диаминопимелатэпимеразу (DapF, EC 5.1.1.7),
j) сверхэкспрессируемый полинуклеотид (ген dapB), который кодирует дигидропиколинатредуктазу (DapB, EC 1.3.1.26), предпочтительно дигидропиколинатредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 172,
k) сверхэкспрессируемый полинуклеотид, который кодирует глюкозо-6-фосфат-дегидрогеназу (EC 1.1.1.49), предпочтительно глюкозо-6-фосфат-дегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 174,
l) сверхэкспрессируемый полинуклеотид (ген zwf), который кодирует субъединицу Zwf глюкозо-6-фосфат-дегидрогеназы (Zwf, EC 1.1.1.49), предпочтительно субъединицу Zwf с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 188,
m) сверхэкспрессируемый полинуклеотид (ген opcA), который кодирует субъединицу OpcA глюкозо-6-фосфат-дегидрогеназы (OpcA, EC 1.1.1.49), предпочтительно субъединицу OpcA с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 190,
n) сверхэкспрессируемый полинуклеотид (ген gnd), который кодирует дегидрогеназу фосфоглюконовой кислоты (Gnd, EC 1.1.1.44), предпочтительно дегидрогеназу фосфоглюконовой кислоты с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 176,
o) инактивированный или аттенуированный полинуклеотид (mqo), который кодирует малат:хинон-оксидоредуктазу (Mqo, EC 1.1.99.16), предпочтительно малат:хинон-оксидоредуктазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 178,
p) инактивированный или аттенуированный полинуклеотид (aceE), который кодирует субъединицу Е1р пируватдегидрогеназного комплекса (AceE, EC 1.2.4.1), предпочтительно субъединицу Е1р с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 180,
q) инактивированный или аттенуированный полинуклеотид (gltA), который кодирует цитратсинтазу (GltA, EC 4.1.3.7), предпочтительно цитратсинтазу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 182,
r) инактивированный или аттенуированный полинуклеотид (mdh), который кодирует малатдегидрогеназу (Mdh, EC 1.1.1.37), предпочтительно малатдегидрогеназу с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 184,
s) инактивированный или аттенуированный полинуклеотид (murE), который кодирует UDP-N-ацетилмурамоилаланил-D-глутамат-2,6-диаминопимелатлигазу (MurE, EC 6.3.2.13), предпочтительно фермент с последовательностью, которая по меньшей мере на 90, 95 или 98%, предпочтительно на 100% идентична последовательности под SEQ ID NO: 186.
Настоящее изобретение также дополнительно соответственно относится к способу получения в избыточном количестве L-аминокислоты, в частности L-лизина, в котором используют такой микроорганизм или такую бактерию.
Упомянутые выше полинуклеотиды и полипептиды, используемые или подлежащие использованию в способе в соответствии с настоящим изобретением, предпочтительно происходят из коринебактерий, в частности из C.glutamicum или C.humireducens, особенно предпочтительно из C.humireducens.
«Сверхэкспрессию» в соответствии с настоящим изобретением в общем необходимо понимать, как увеличение внутриклеточной концентрации или активности рибонуклеиновой кислоты, белка (полипептида) или фермента, которые кодируются соответствующей ДНК, в микроорганизме по сравнению с исходным штаммом (родительским штаммом) или штаммом дикого типа. Термин исходный штамм (родительский штамм) означает штамм, при воздействии на который в результате достигают сверхэкспрессии.
Увеличение концентрации или активности может быть достигнуто, например, путем увеличения числа копий соответствующих кодирующих полинуклеотидов, хромосомных или внехромосомных, по меньшей мере на одну копию.
Широко используемый способ увеличения числа копий предусматривает введение соответствующего кодирующего полинуклеотида в вектор, предпочтительно плазмиду, которая реплицируется в микроорганизме, в частности в коринеформных бактериях. Кроме того, в качестве векторов можно использовать транспозоны, инсерционные элементы (IS-элементы) или фаги. Множество подходящих векторов известно из уровня техники.
Другим широко применяемым способом достижения сверхэкспрессии является способ амплификации гена в хромосоме. В данном способе по меньшей мере одну дополнительную копию представляющего интерес полинуклеотида встраивают в хромосому коринеформной бактерии. Такие способы амплификации описаны, например, в WO 03/014330 или WO 03/040373.
Дополнительный способ достижения сверхэкспрессии предусматривает связывание соответствующего гена или аллеля функциональным образом (функциональное связывание) с промотором или кассетой экспрессии. Подходящие промоторы для Corynebacterium glutamicum, описаны, например, на фиг. 1 обзорной статьи Patek и соавт. (Journal of Biotechnology 104(1-3), 311-323 (2003)) и в обширном обзоре, таком как "Handbook of Corynebacterium glutamicum" (Eds.: Lothar Eggeling and Michael Bott, CRC Press, Boca Raton, US (2005)), или в книге "Corynebacteria, Genomics and Molecular Biology" (Ed.: Andreas Burkovski, Caister Academic Press, Norfolk, UK (2008)). Таким же образом можно использовать варианты промотора dapA, например, промотор A25, описанный в публикации Vasicova и соавт. (Journal of Bacteriology 181, 6188-6191 (1999)). Кроме того, можно использовать промотор gap из Corynebacterium glutamicum (EP 06007373). И наконец, можно использовать хорошо известные промоторы T3, T7, SP6, M13, lac, tac и trc, описанные в публикациях Amann и соавт. (Gene 69(2), 301-315 (1988)) и Amann and Brosius (Gene 40(2-3), 183-190 (1985)). Такой промотор может быть встроен, например, выше соответствующего гена, как правило, на расстоянии приблизительно 1-500 пар нуклеотидных оснований от стартового кодона.
Оказывая воздействия посредством сверхэкспрессии повышают активность или концентрацию соответствующего полипептида предпочтительно по меньшей мере на 10%, 25%, 50%, 75%, 100%, 150%, 200%, 300%, 400% или 500%, предпочтительно не более чем на 1000% или 2000%, исходя из активности или концентрации указанного полипептида в штамме до осуществления воздействия, которое в результате приводит к сверхэкспрессии.
Концентрацию белка можно определить с помощью 1- и 2-мерного разделения белков в геле и последующего оптического определения концентрации белка в геле с использованием соответствующего аналитического программного обеспечения. Общепринятый способ подготовки геля с белками для коринеформных бактерий и идентификации указанных белков представляет собой процедуру, описанную в Hermann и соавт. (Electrophoresis, 22:1712-23 (2001)). Концентрацию белка также можно определить с помощью вестерн-блот-гибридизации с использованием антитела, специфичного для белка, подлежащего обнаружению (Sambrook et al., Molecular Cloning: a laboratory manual, 2nd Ed. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989) и последующей оптической оценкой с использованием соответствующего программного обеспечения для определения концентрации (Lohaus and Meyer (1998) Biospektrum 5:32-39; Lottspeich, Angewandte Chemie 38: 2630-2647 (1999)). Активность можно определить с помощью подходящего ферментного анализа.
«Аттенуация» в соответствии с настоящим изобретением относится к снижению внутриклеточной концентрации или активности рибонуклеиновой кислоты, белка (полипептида) или фермента, которые кодируются соответствующей ДНК, в микроорганизме по сравнению с исходным штаммом (родительским штаммом) или штаммом дикого типа. Исходный штамм (родительский штамм) относится к штамму, при воздействии на который в результате осуществляется аттенуация.
Аттенуация может быть достигнута путем снижения экспрессии полипептида, например, путем использования слабого промотора или использования аллеля, кодирующего полипептид, характеризующийся низкой активностью, при этом необязательно данные средства воздействия могут быть объединены. Аттенуация также может быть достигнута посредством полного блокирования экспрессии полипептида, например, путем инактивирования кодирующего гена.
Оказывая воздействие посредством аттенуации уменьшают активность или концентрацию соответствующего полипептида предпочтительно по меньшей мере на 10%, 25%, 50%, 75%, предпочтительно не более чем на 100%, исходя из активности или концентрации указанного полипептида в штамме до осуществления воздействия, которое в результате приводит к аттенуации. В предпочтительном варианте осуществления аттенуация предусматривает практически полную инактивацию экспрессии соответствующего полипептида.
Устойчивые к ингибированию конечным продуктом ферменты по отношению к продуцированию аминокислот в общем необходимо понимать как ферменты, которые по сравнению с исходной формой характеризуются пониженной чувствительностью к ингибированию продуцируемыми L-аминокислотами и/или их аналогами.
В частности, под устойчивыми к ингибированию конечным продуктом аспартаткиназами (LysCFBR) подразумевают аспартаткиназы, которые по сравнению с исходной формой демонстрируют меньшую чувствительность к ингибированию смесями лизина и треонина, или смесями AEC (аминоэтилцистеина) и треонина, или лизина в отдельности, или AEC в отдельности. С целью получения лизина предпочтительно используют соответствующие штаммы, которые содержат такие устойчивые к ингибированию конечным продуктом или десенсибилизированные аспартаткиназы.
Например, следующие устойчивые к ингибированию конечным продуктом аспартаткиназы из C.glutamicum известны из ссылочных публикаций: A279T, A279V, S301F, S301Y, T308I, T311I, R320G, G345D, S381F. По отношению к устойчивым к ингибированию конечным продуктом аспартаткиназам из C.glutamicum необходимо также сослаться на следующие публикации: JP1993184366-A, JP1994062866-A, JP1994261766-A, JP1997070291-A, JP1997322774-A, JP1998165180-A, JP1998215883-A, US5688671-A, EP0387527, WO00/63388, US3732144, JP6261766, Jetten и соавт. (1995; Applied Microbiology Biotechnology 43: 76-82). Устойчивые к ингибированию конечным продуктом аспартаткиназы из C.glutamicum депонированы в NCBI GenBank под следующими номерами доступа: E05108, E06825, E06826, E06827, E08177, E08178, E08179, E08180, E08181, E08182, E12770, E14514, E16352, E16745, E16746, I74588, I74589, I74590, I74591, I74592, I74593, I74594, I74595, I74596, I74597, X57226, L16848, L27125.
В соответствии с настоящим изобретением предпочтительно используют следующие устойчивые к ингибированию конечным продуктом аспартаткиназы из C.humireducens: D274Y, A279E, S301Y, T308I, T311I, G359D.
Для получения треонина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом гомосерин-дегидрогеназу (HomFBR).
Для получения изолейцина и для получения валина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом ацетолактатсинтазу.
Для продуцирования лейцина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом изопропилмалатсинтазу (LeuAFBR).
Для продуцирования пролина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом глутамат-5-киназу (ProBFBR).
Для получения аргинина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом орнитинкарбамоилтрансферазу (ArgFFBR).
Для получения серина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом D-3-фосфоглицератдегидрогеназу (SerAFBR).
Для получения метионина предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом D-3-фосфоглицератдегидрогеназу (SerAFBR) и/или устойчивые к ингибированию конечным продуктом пируваткарбоксилазы (PycFBR).
Для получения триптофана предпочтение также отдают использованию штаммов, содержащих соответствующую устойчивую к ингибированию конечным продуктом фосфо-2-дегидро-3-дезоксигептонатальдолазу (AroGFBR или AroHFBR).
По отношению к еще более предпочтительным свойствам штаммов C.humireducens, продуцирующих в избыточном количестве L-аминокислоты, которые подлежат использованию в соответствии с настоящим изобретением, необходимо сослаться на публикацию Wu и соавт. (2011), которую цитировали выше, и другие вышеупомянутые публикации.
Микроорганизмы в соответствии с настоящим изобретением, в частности бактерии рода Corynebacterium, можно культивировать непрерывно - как описано, например, в WO 05/021772 - или прерывисто посредством периодического процесса (периодическое культивирование или периодический способ), или посредством процесса периодического культивирования с подпиткой или повторяющегося периодического культивирования с подпиткой c целью продуцирования L-лизина. Общий обзор известных способов культивирования доступен в учебнике Chmiel (Bioprozesstechnik 1.
Figure 00000002
in die Bioverfahrenstechnik [Bioprocess Technology 1. Introduction to Bioprocess Technology] (Gustav Fischer Verlag, Stuttgart, 1991)) или в пособии Storhas (Bioreaktoren und periphere Einrichtungen [Bioreactors and Peripheral Devices] (Vieweg Verlag, Braunschweig/Wiesbaden, 1994)).
Подлежащие использованию культуральная среда или среда для ферментации должна надлежащим образом удовлетворять потребностям конкретных штаммов. Описания питательных сред для разных микроорганизмов представлены в справочнике «Manual of Methods for General Bacteriology» от Американского общества по бактериологии (American Society for Bacteriology) (Вашингтон, Округ Колумбия, США, 1981). Выражения культуральная среда и среда для ферментации или просто среда являются взаимозаменяемыми.
Используемые источники углерода могут представлять собой сахара и углеводы, такие как глюкоза, сахароза, лактоза, фруктоза, мальтоза, мелассы, содержащие сахарозу растворы, полученные при переработке сахарной свеклы или сахарного тростника, крахмал, гидролизат крахмала и целлюлозу, масла и жиры, такие как соевое масло, подсолнечное масло, арахисовое масло и кокосовый жир, жирные кислоты, такие как пальмитиновая кислота, стеариновая кислота и линолевая кислота, спирты, такие как глицерин, метанол и этанол, а также органические кислоты, такие как уксусная кислота или молочная кислота.
В качестве источников азота можно использовать органические азотсодержащие соединения, такие как пептоны, дрожжевой экстракт, мясной экстракт, солодовый экстракт, жидкий кукурузный экстракт, соевая мука и мочевина, или неорганические соединения, такие как сульфат аммония, хлорид аммония, фосфат аммония, карбонат аммония и нитрат аммония. Источники азота можно использовать по отдельности или в виде смеси.
Используемые источники фосфора могут представлять собой фосфорную кислоту, дигидрофосфат калия или гидроортофосфат калия или соответствующие натрийсодержащие соли.
Культуральная питательная среда должна дополнительно содержать соли, например, в виде хлоридов или сульфатов металлов, таких как натрий, калий, магний, кальций и железо, например, сульфат магния или сульфат железа, которые необходимы для роста. Наконец, незаменимые факторы роста, такие как аминокислоты, например, гомосерин, и витамины, например, тиамин, биотин или пантотеновая кислота, можно использовать в дополнение к вышеуказанным веществам.
Указанное сырье можно вносить в культуральную среду в виде единой смеси или можно подавать надлежащим образом в ходе культивирования.
Значение pH в культуре можно контролировать посредством применения надлежащим способом основных соединений, таких как гидроксид натрия, гидроксид калия, аммиак или водный раствор аммиака, или кислых соединений, таких как фосфорная кислота или серная кислота. Как правило, pH доводят до значения от 6,0 до 9,0, предпочтительно от 6,5 до 8. Для контроля пенообразования можно использовать пеногасители, например, сложные полигликолевые эфиры жирных кислот. Для поддержания стабильности плазмид необходимо добавлять в среду селективные вещества, такие как, например, антибиотики. Для поддержания аэробных условий кислород или кислородные газовые смеси, например, воздух, подают в культуру. Также возможно использование жидкостей, обогащенных перекисью водорода. При необходимости ферментацию проводят при повышенном давлении, например, при давлении от 0,03 до 0,2 МПа. Температура культуры обычно составляет от 20°C до 45°C и предпочтительно от 25°C до 40°C. В периодических процессах культивирование продолжают до образования максимального количества требуемой L-аминокислоты. Данной цели достигают, как правило, в пределах от 10 часов до 160 часов. В непрерывных способах возможны более длительные периоды культивирования. Результатом активности бактерий является повышение концентрации (накопление) L-аминокислоты в ферментационной среде и/или в бактериальных клетках.
Примеры подходящих ферментационных сред приведены, в том числе, в описаниях патентов US 5770409, US 5840551 и US 5990350 или US 5275940.
Анализ L-аминокислот для определения концентрации в один или более моментов времени в ходе ферментации можно осуществить путем разделения L-аминокислот с помощью ионообменной хроматографии, предпочтительно катионообменной хроматографии, с последующим пост-колоночным получением производных с использованием нингидрина, как описано в публикации Spackman и соавт. (Analytical Chemistry 30: 1190-1206 (1958)). Также возможным является использование орто-фталальдегида вместо нингидрина для пост-колоночного получения производных. Обзорную статью по ионообменной хроматографии можно найти в публикации Pickering (LC GC (Magazine of Chromatographic Science) 7(6), 484-487 (1989)).
Также можно осуществить предколоночное получение производных, например, с помощью ортофталевого альдегида или фенилизотиоцианата, и разделить полученные аминокислотные производные с помощью обращенно-фазовой хроматографии (RP), предпочтительно в форме высокоэффективной жидкостной хроматографии (HPLC). Такой способ описан, например, в публикации Lindroth и соавт. (Analytical Chemistry 51: 1167-1174 (1979)).
Детекцию осуществляли фотометрически (поглощение, флуоресценция).
Обзор анализа аминокислот можно найти, среди прочего, в учебнике "Bioanalytik", авторы Lottspeich and Zorbas (Spektrum Akademischer Verlag, Heidelberg, Germany 1998).
Соответственно, настоящее изобретение также относится к способу получения L-аминокислоты, характеризующемуся тем, что осуществляют следующие стадии:
a) ферментация микроорганизмов в соответствии с настоящим изобретением, в частности коринеформных бактерий, предпочтительно бактерий рода Corynebacterium, особенно предпочтительно видов Corynebacterium glutamicum или Corynebacterium humireducens, в подходящей питательной среде, и
b) накопление L-аминокислот в питательной среде и/или в клетках упомянутых бактерий.
Продукт, содержащий L-аминокислоты, затем восстанавливают, или получают, или извлекают в жидкой или твердой форме.
Результатом проведения ферментации является ферментационный бульон, который содержит соответствующую L-аминокислоту.
Ферментационный бульон означает ферментационную среду или питательную среду, в которой микроорганизм культивировали в течение определенного периода времени и при определенной температуре. Ферментационная среда или среды, используемые при ферментации, содержит/содержат все вещества или компоненты, которые обеспечивают размножение микроорганизма и образование требуемой L-аминокислоты.
Соответственно, по завершении ферментации полученный в результате бульон, содержит:
a) биомассу (клеточную массу) микроорганизма, при этом указанная биомасса образовалась в результате размножения клеток указанного микроорганизма,
b) L-аминокислоту, образовавшуюся в ходе ферментации,
c) органические побочные продукты, образовавшиеся в ходе ферментации, и
d) компоненты используемой ферментационной среды или сырья, такие как, например, витамины, такие как биотин, или соли, такие как сульфат магния, которые не были поглощены в ходе ферментации.
Органические побочные продукты включают вещества, которые продуцируются используемыми микроорганизмами при ферментации в дополнение к требуемому соединению, и они необязательно секретируются клетками. Они также включают сахара, такие как, например, трегалоза.
Ферментационный бульон удаляют из сосуда для культивирования или резервуара для ферментации, при необходимости собирают, и используют для получения продукта, содержащего L-аминокислоты, в жидкой или твердой форме. Выражение «извлечение продукта, содержащего L-аминокислоты» также используют для данного контекста. В самом простом случае ферментационный бульон, содержащий L-аминокислоты, собственно представляет собой извлеченный продукт.
С помощью одного или более воздействий, выбранных из группы, включающей
a) от частичного (от >0% до <80%) до полного (100%) или практически полного (≥80%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99%) удаления воды,
b) от частичного (от >0% до <80%) до полного (100%) или практически полного (≥80%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99%) удаления биомассы, при этом последнюю необязательно инактивируют перед удалением,
c) от частичного (от >0% до <80%) до полного (100%) или практически полного (≥80%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99%, ≥99,3%, ≥99,7%) удаления органических побочных продуктов, образовавшихся в ходе ферментации, и
d) от частичного (>0%) до полного (100%) или практически полного (≥80%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99%, ≥99,3%, ≥99,7%) удаления компонентов используемой ферментационной среды или сырья - тех компонентов, которые не были поглощены в ходе ферментации,
из ферментационного бульона, обеспечивается концентрирование или очистка L-аминокислоты. Посредством данного способа выделяют продукты с требуемым содержанием L-аминокислоты.
Удаление воды (воздействие a) от частичного (от >0% до <80%) до полного (100%) или практически полного (от ≥80% до <100%) также обозначено как высушивание.
Полное или практически полное удаление воды, биомассы, органических побочных продуктов и непоглощенных компонентов используемой ферментационной среды дает в результате чистые (≥80% по весу, ≥90% по весу) или высокой степени очистки (≥95% по весу, ≥97% по весу ≥99% по весу) формы продукта на основе L-аминокислот. Для воздействий, упомянутых в a), b), c) и d), доступно множество протоколов, доступных из предшествующего уровня техники.
Демонстрационные примеры
Пример 1. Получение аланина и валина с помощью C.humireducens
Для оценки получения аланина и валина штамм C.humireducens (DSM 45392) культивировали во встряхиваемой плоскодонной колбе. Для этой цели штамм C.humireducens инкубировали в 10 мл жидкой среды BHI (Brain Heart Infusion; Merck) (37 г/л H2O) при 37°C, при 200 об./мин. в течение 24 ч. в качестве предварительной культуры. Затем 10 мл среды во встряхиваемой колбе инокулировали при OD660 0,2 и культивировали при 37°C при 200 об./мин. в течение 48 ч. Для приготовления указанной среды 20 г сульфата аммония, 0,4 г MgSO4*7H2O, 0,6 г KH2PO4 и 10 г дрожжевого экстракта растворяли в 750 мл H2O. Значение pH раствора доводили до 7,8 с помощью 20% NH4OH и затем раствор автоклавировали. Затем добавляли 4 мл раствора витаминов (значение pH 7 доводили при помощи NH4OH), включающего 0,25 г/л тиамина, 50 мг/л цианокобаламина, 25 мг/л биотина и 1,25 г/л пиридоксина. Кроме того, добавляли 140 мл стерильно фильтрованного 50% раствора глюкозы и 50 г автоклавированного сухого CaCO3, а затем объем среды доводили до одного литра.
После культивирования супернатант четырех параллельных культур анализировали в каждом случае с помощью HPLC для определения содержания аланина, глицина и валина с пределом обнаружения ≥0,01 г/л.
Штамм C.humireducens после культивирования в течение 48 ч. в среде во встряхиваемом флаконе при 37°C, 200 об./мин. в масштабе встряхиваемого флакона обеспечивал приблизительно 0,81 г/л аланина (выход чистого продукта: 0,011 галанина/гглюкозы) и 1,6 г/л валина (выход чистого продукта: 0,022 гвалина/гглюкозы) (Табл. 1). Глицин продуцировался лишь в небольших количествах в качестве побочного продукта.
Таблица 1. Аналитические данные из эксперимента с культивированием во встряхиваемой колбе со штаммом C.humireducens. Показаны значения, полученные после культивирования клеток, и данные для незасеянной среды.
Аланин (г/л) Валин (г/л)
C.humireducens 1,27 1,9
Питательная среда без клеток 0,46 0,3
Пример 2. Анализ эффективности получения глутамата
Для анализа эффективности получения L-глутамата штамм C.humireducens (DSM 45392) культивировали во встряхиваемой колбе. Для этой цели штамм C.humireducens инкубировали в 10 мл жидкой среды BHI (Brain Heart Infusion; Merck) (37 г/л H2O) при 37°C, при 200 об./мин. в течение 24 ч. в качестве предварительной культуры. Затем 10 мл среды во встряхиваемой колбе инокулировали при OD660 0,2 и культивировали при 37°C при 200 об./мин. в течение 48 ч. Для приготовления указанной среды 20 г сульфата аммония, 0,4 г MgSO4*7H2O, 0,6 г KH2PO4 и 10 г дрожжевого экстракта растворяли в 750 мл H2O. Значение pH раствора доводили до 7,8 с помощью 20% NH4OH и затем раствор автоклавировали. Затем добавляли 4 мл раствора витаминов (значение pH 7 доводили при помощи NH4OH), включающего 0,25 г/л тиамина, 50 мг/л цианокобаламина, 25 мг/л биотина и 1,25 г/л пиридоксина. Кроме того, добавляли 140 мл стерильно фильтрованного 50% раствора глюкозы и 50 г автоклавированного сухого CaCO3. Затем добавляли 5 мл 400 мМ стерильно фильтрованного маточного раствора, а затем среду доводили до одного литра.
После культивирования супернатант четырех параллельных культур анализировали в каждом случае с помощью HPLC для определения содержания глутамата с пределом обнаружения ≥0,01 г/л.
Штамм C.humireducens после культивирования в течение 48 ч. в среде во встряхиваемом флаконе при 37°C, 200 об./мин. в масштабе встряхиваемого флакона обеспечивал приблизительно 1,8 (+/-0,6) г/л L-глутамата. Начальная концентрация L-глутамата в среде составляла 0,78 (+/-0,1) г/л. Глицин продуцировался лишь в небольших количествах в качестве побочного продукта.
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ПЕРЕЧЕНЬ ПОСЛЕДОВАТЕЛЬНОСТЕЙ
<110> Эвоник Индастриз АГ
<120> Способ получения L-аминокислот с помощью коринебактерий с применением системы расщепления глицина
<130> 201400112
<160> 202
<170> PatentIn версии 3.5
<210> 1
<211> 1219
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(918)
<223> ген mcbR
<400> 1
ctactcggtg gcttcctcct cagcctcctg ggggtcgacg tggccggcgg aggcctgatc 60
ttcagcttcc tcacctgtct gctcggtgcg gtgatcctgc tgaccatcgt caacatggtg 120
accggtcgca gccggagcta gcctgcgggt ccccgcatga acccgcccct cggggcgggt 180
tttttcatgc ccgtatgcag ggcgactgtc aaaagacgta ccggtttgtc tatattgtgt 240
gggagaggta gtcccgaacg atcccggaaa ggaaccggta cccacgtgtc cgcaggaaca 300
gtg agg aag aag acg acg acc gcg cgg cgt cgc aac cga ccg agc ccg 348
Val Arg Lys Lys Thr Thr Thr Ala Arg Arg Arg Asn Arg Pro Ser Pro
1 5 10 15
cgt cag cga ctg ctc gac tcg gcg acc aac ctg ttc acc acg gag ggc 396
Arg Gln Arg Leu Leu Asp Ser Ala Thr Asn Leu Phe Thr Thr Glu Gly
20 25 30
atc cgg gtc atc ggc atc gac cgg atc ctg cgg gag gcg gac gtc gcc 444
Ile Arg Val Ile Gly Ile Asp Arg Ile Leu Arg Glu Ala Asp Val Ala
35 40 45
aag gcg tcc ctg tac tcc ctc ttc ggc tcg aag gac gcc ctg gtc atc 492
Lys Ala Ser Leu Tyr Ser Leu Phe Gly Ser Lys Asp Ala Leu Val Ile
50 55 60
tcc tac ctg gag aac ctc gac gag cag tac cgg acg gcc tgg gag gag 540
Ser Tyr Leu Glu Asn Leu Asp Glu Gln Tyr Arg Thr Ala Trp Glu Glu
65 70 75 80
cgg acg aag ggg atg acg gac gcc gag gac aag atc ctc gcc ttc ttc 588
Arg Thr Lys Gly Met Thr Asp Ala Glu Asp Lys Ile Leu Ala Phe Phe
85 90 95
gac ctc gcc atc gcg gag gag ccg ggc aag gag ttc cgc ggc tcc cac 636
Asp Leu Ala Ile Ala Glu Glu Pro Gly Lys Glu Phe Arg Gly Ser His
100 105 110
ttc cag aac gcc gcc aac gag tac ccg cgc ccg gag acc gag tcc gag 684
Phe Gln Asn Ala Ala Asn Glu Tyr Pro Arg Pro Glu Thr Glu Ser Glu
115 120 125
cac ggc atc gtc gcc gcc tgc cgg gag cac cgc cgc tgg atg cac cag 732
His Gly Ile Val Ala Ala Cys Arg Glu His Arg Arg Trp Met His Gln
130 135 140
acg atg acg gac ctg ctc acc gcg aag aac ggc tac ccc tcc gcc acg 780
Thr Met Thr Asp Leu Leu Thr Ala Lys Asn Gly Tyr Pro Ser Ala Thr
145 150 155 160
cag gcg aac cag ctg ctc atc ttc ctc gac ggc ggc ctg gcg ggc gcg 828
Gln Ala Asn Gln Leu Leu Ile Phe Leu Asp Gly Gly Leu Ala Gly Ala
165 170 175
cgc ttc gcc cgg gag gtc act ccg ctg cag acg gcc cgt gat ctg gcc 876
Arg Phe Ala Arg Glu Val Thr Pro Leu Gln Thr Ala Arg Asp Leu Ala
180 185 190
cgt cag atg ctg tcg gct cct ccg gcg gac tac tcg atc tag 918
Arg Gln Met Leu Ser Ala Pro Pro Ala Asp Tyr Ser Ile
195 200 205
cgttcttccg cgcctccagc ttcgccctcc accgctcgac gcgggcgttc gtctcctccc 978
gctgcagggc gctgcgggcc tgctgacgct ccttctcgag cgccttccgc tccgccttct 1038
ccgccgccag ccgctcgcga cgttcggcct tctcggcctt cagttcgcgg ctgatctcgg 1098
ggatccgcca cggctgcacg gcgcccactg ctttccgacg ccgcgcgtcc cacttcacct 1158
gccgcgcctc acgcgccgcc gcccgggcct tctcccggcc ctgccggtgc agctcgtgga 1218
a 1219
<210> 2
<211> 205
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 2
Val Arg Lys Lys Thr Thr Thr Ala Arg Arg Arg Asn Arg Pro Ser Pro
1 5 10 15
Arg Gln Arg Leu Leu Asp Ser Ala Thr Asn Leu Phe Thr Thr Glu Gly
20 25 30
Ile Arg Val Ile Gly Ile Asp Arg Ile Leu Arg Glu Ala Asp Val Ala
35 40 45
Lys Ala Ser Leu Tyr Ser Leu Phe Gly Ser Lys Asp Ala Leu Val Ile
50 55 60
Ser Tyr Leu Glu Asn Leu Asp Glu Gln Tyr Arg Thr Ala Trp Glu Glu
65 70 75 80
Arg Thr Lys Gly Met Thr Asp Ala Glu Asp Lys Ile Leu Ala Phe Phe
85 90 95
Asp Leu Ala Ile Ala Glu Glu Pro Gly Lys Glu Phe Arg Gly Ser His
100 105 110
Phe Gln Asn Ala Ala Asn Glu Tyr Pro Arg Pro Glu Thr Glu Ser Glu
115 120 125
His Gly Ile Val Ala Ala Cys Arg Glu His Arg Arg Trp Met His Gln
130 135 140
Thr Met Thr Asp Leu Leu Thr Ala Lys Asn Gly Tyr Pro Ser Ala Thr
145 150 155 160
Gln Ala Asn Gln Leu Leu Ile Phe Leu Asp Gly Gly Leu Ala Gly Ala
165 170 175
Arg Phe Ala Arg Glu Val Thr Pro Leu Gln Thr Ala Arg Asp Leu Ala
180 185 190
Arg Gln Met Leu Ser Ala Pro Pro Ala Asp Tyr Ser Ile
195 200 205
<210> 3
<211> 1527
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1227)
<223> ген thrB
<400> 3
agaacaccta cgcgaacctg ccgatcgccg acctcggcga cgtgcgcacc cgctaccaca 60
tcgacatgga ggtcgaggac cgcaccggtg tcctcgccga gctgtccgcg atcttcgcca 120
cccacgacat ctccctcaag accgtccgcc aggaggaggc cggcgaccac gcccgcctca 180
tcgtcgtcac gcattccgca cgtgaggccg acctggccgc caccgtcgag gacctgaaga 240
aggccccggc ggtcaagtcc gtcgacagcg tcctgcgact catctaggga gcgttgagct 300
gtg agc atc cag ctt gag gtg ggc acc cgg gcc acc gtc acc gtc ccc 348
Val Ser Ile Gln Leu Glu Val Gly Thr Arg Ala Thr Val Thr Val Pro
1 5 10 15
ggc tcc tcc gcg aac ctg ggc ccc gga ttc gac acc ctc ggc ctg gcc 396
Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala
20 25 30
gtg ggc atc tac gac acc gtc gag gtc gag gtc atc gag tcg ggc ctc 444
Val Gly Ile Tyr Asp Thr Val Glu Val Glu Val Ile Glu Ser Gly Leu
35 40 45
gag gtc gag gtc ttc ggc gag ggc gag gac gac ctg ccc cgc gac ggt 492
Glu Val Glu Val Phe Gly Glu Gly Glu Asp Asp Leu Pro Arg Asp Gly
50 55 60
tcc cac ctg gtg gtc aag gcc atc cgg tcg ggc ctc aac gcc gcc cac 540
Ser His Leu Val Val Lys Ala Ile Arg Ser Gly Leu Asn Ala Ala His
65 70 75 80
gcc gag gcc ccg ggc ctg cgc gtg gtg tgc acc aac gcc atc ccg cag 588
Ala Glu Ala Pro Gly Leu Arg Val Val Cys Thr Asn Ala Ile Pro Gln
85 90 95
tcc cgt ggc ctg ggc tcc tcc gcc gcc gca gcc gtc gcg gga gtt ctg 636
Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Leu
100 105 110
gcc gcc aac ggt ctg gcg ggc aac ccg ctg acc acc gac cag gtg gtc 684
Ala Ala Asn Gly Leu Ala Gly Asn Pro Leu Thr Thr Asp Gln Val Val
115 120 125
cag ctg tcc tcc gcc ttc gag ggg cac ccc gac aac gcc gcg gcc tcc 732
Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser
130 135 140
gtc ctc ggt tcc gcc gtg gtc tcc tgg acc acg acc ccg gtc gac ggc 780
Val Leu Gly Ser Ala Val Val Ser Trp Thr Thr Thr Pro Val Asp Gly
145 150 155 160
tcg cag ccg ggg tac cgg gcg gtg gcg gtg gac gtc gac aag cgc atc 828
Ser Gln Pro Gly Tyr Arg Ala Val Ala Val Asp Val Asp Lys Arg Ile
165 170 175
cgg gcg acg gct ctc gtg ccg gac ttc cac gcc tcc acc cag gcg gtg 876
Arg Ala Thr Ala Leu Val Pro Asp Phe His Ala Ser Thr Gln Ala Val
180 185 190
cgt cgc gtg ctg ccg agc cac gtc acc cac acg gac gcg cgt ttc aac 924
Arg Arg Val Leu Pro Ser His Val Thr His Thr Asp Ala Arg Phe Asn
195 200 205
gtc tcg cgc acc gcg gtg atg acc gtg gcg ctg cag aac cac ccg gag 972
Val Ser Arg Thr Ala Val Met Thr Val Ala Leu Gln Asn His Pro Glu
210 215 220
ctg ctg tgg gag ggc acc cgt gac cgc ctg cac cag ccc tac cgc gcc 1020
Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala
225 230 235 240
gac gtg ctg ccg gtg acc gcc gag tgg gtc aac cgt ctg cgc aac cgc 1068
Asp Val Leu Pro Val Thr Ala Glu Trp Val Asn Arg Leu Arg Asn Arg
245 250 255
ggt tac gcc gcg tac ctc tcc ggc gcc ggc ccg acc tgc atg gtc ctg 1116
Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Cys Met Val Leu
260 265 270
tcg gtc gac ccg atc gag gag gcc atc ctc gag gag gcc cgc gcc gcc 1164
Ser Val Asp Pro Ile Glu Glu Ala Ile Leu Glu Glu Ala Arg Ala Ala
275 280 285
ggt ctg cgc gtc ctc gag ctg gag gtc gcc ggt ccg gcg acc gtc gag 1212
Gly Leu Arg Val Leu Glu Leu Glu Val Ala Gly Pro Ala Thr Val Glu
290 295 300
gtc agc cgg gtc tga cctaccggtc gtcggcgggg gtccccctgt cggcgaccac 1267
Val Ser Arg Val
305
cgagcacgcg cccgagggct ggaaggggcg caggctcagc cggacggggg cggagccgac 1327
ggactcctgc aggaaaccct cgtggatggc gcagatcatg agcgagggtc ggtgcccctc 1387
ggcgacgaag gggcacgcct gcagctcgat gtcgaccgac tccgcgtcgg tgcccccgtc 1447
gccctgctcc ctgcgcatgg tcgggtcgaa acccagctca cggagggtgt ggtggagacg 1507
gtcgagcgcc gtgtccatgg 1527
<210> 4
<211> 308
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 4
Val Ser Ile Gln Leu Glu Val Gly Thr Arg Ala Thr Val Thr Val Pro
1 5 10 15
Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala
20 25 30
Val Gly Ile Tyr Asp Thr Val Glu Val Glu Val Ile Glu Ser Gly Leu
35 40 45
Glu Val Glu Val Phe Gly Glu Gly Glu Asp Asp Leu Pro Arg Asp Gly
50 55 60
Ser His Leu Val Val Lys Ala Ile Arg Ser Gly Leu Asn Ala Ala His
65 70 75 80
Ala Glu Ala Pro Gly Leu Arg Val Val Cys Thr Asn Ala Ile Pro Gln
85 90 95
Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Leu
100 105 110
Ala Ala Asn Gly Leu Ala Gly Asn Pro Leu Thr Thr Asp Gln Val Val
115 120 125
Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser
130 135 140
Val Leu Gly Ser Ala Val Val Ser Trp Thr Thr Thr Pro Val Asp Gly
145 150 155 160
Ser Gln Pro Gly Tyr Arg Ala Val Ala Val Asp Val Asp Lys Arg Ile
165 170 175
Arg Ala Thr Ala Leu Val Pro Asp Phe His Ala Ser Thr Gln Ala Val
180 185 190
Arg Arg Val Leu Pro Ser His Val Thr His Thr Asp Ala Arg Phe Asn
195 200 205
Val Ser Arg Thr Ala Val Met Thr Val Ala Leu Gln Asn His Pro Glu
210 215 220
Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala
225 230 235 240
Asp Val Leu Pro Val Thr Ala Glu Trp Val Asn Arg Leu Arg Asn Arg
245 250 255
Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Cys Met Val Leu
260 265 270
Ser Val Asp Pro Ile Glu Glu Ala Ile Leu Glu Glu Ala Arg Ala Ala
275 280 285
Gly Leu Arg Val Leu Glu Leu Glu Val Ala Gly Pro Ala Thr Val Glu
290 295 300
Val Ser Arg Val
305
<210> 5
<211> 2238
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1938)
<223> ген pgi
<400> 5
caggcgatgt aggactcctc gtcggcccag cgggtgacca cgaagtagcg gttctccccg 60
gccacggggc ggagaagctg gaaaccctcg aagccgggtg cctggtcgac ggcgttcttc 120
cgggcggcga agcgctcctc cagctgcggg ccggcgccct cggggacggt gatggcgttg 180
attttcacga tgctcatgcc ccccattgtg cccaaccggc cgcttccccg gcggacaccc 240
gggaaagtca ctagattcgt gagtgtcccg aacatatccc cgcattgagg agcccgaccc 300
atg gcc gac atc acc acc tcc gct gaa tgg aag aac ctg gag tcc gag 348
Met Ala Asp Ile Thr Thr Ser Ala Glu Trp Lys Asn Leu Glu Ser Glu
1 5 10 15
ttc gag cgc acc cag ggt cag acc ctg cgg gag ctg ttc gcc gcg gat 396
Phe Glu Arg Thr Gln Gly Gln Thr Leu Arg Glu Leu Phe Ala Ala Asp
20 25 30
ccg cag cgt gcg gag aag ctg acc ttc gat gcg gcc ggt ctc cac gtc 444
Pro Gln Arg Ala Glu Lys Leu Thr Phe Asp Ala Ala Gly Leu His Val
35 40 45
gac ctc tcc aag aac ctg ctt gac gac gcc acc gtg gcc tcc ctc gtc 492
Asp Leu Ser Lys Asn Leu Leu Asp Asp Ala Thr Val Ala Ser Leu Val
50 55 60
gcc ctc gcc aag gag gcg cag ctc acc gag gcc atc gag gcg atg ttc 540
Ala Leu Ala Lys Glu Ala Gln Leu Thr Glu Ala Ile Glu Ala Met Phe
65 70 75 80
agc ggc gtg cac atc aac aac acc gag gac cgc gcc gtg ctg cac acc 588
Ser Gly Val His Ile Asn Asn Thr Glu Asp Arg Ala Val Leu His Thr
85 90 95
gcc ctg cgt ctg ccg gtc gag gag gac ctc gag gtc gac ggc cag gac 636
Ala Leu Arg Leu Pro Val Glu Glu Asp Leu Glu Val Asp Gly Gln Asp
100 105 110
gtc gcc gcc gac gtc cac gag gtg ctc ggc cgc atg cgc gac ttc gcc 684
Val Ala Ala Asp Val His Glu Val Leu Gly Arg Met Arg Asp Phe Ala
115 120 125
tcc tcc ctg cgt gcg ggc acc tgg ctc ggc cac acc ggc cgc acc atc 732
Ser Ser Leu Arg Ala Gly Thr Trp Leu Gly His Thr Gly Arg Thr Ile
130 135 140
aag aag gtc gtc aac atc ggc atc ggc ggc tcc gac ctg ggc ccg gcc 780
Lys Lys Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala
145 150 155 160
atg gcc gcc cgg gcg ctg cgc tcc tac gcc acc gcc ggc atc acc gcc 828
Met Ala Ala Arg Ala Leu Arg Ser Tyr Ala Thr Ala Gly Ile Thr Ala
165 170 175
gag ttc gtc tcc aac gtc gac ccg gcc gac atg tcc gcc acc ctc gac 876
Glu Phe Val Ser Asn Val Asp Pro Ala Asp Met Ser Ala Thr Leu Asp
180 185 190
gag ctc gac ccc gag tcg acc ctg ttc atc atc gcc tcc aag acc ttc 924
Glu Leu Asp Pro Glu Ser Thr Leu Phe Ile Ile Ala Ser Lys Thr Phe
195 200 205
acc acc cag gag acc ctc gcc aac gcg cac gcc gcg aag cgc tgg ctg 972
Thr Thr Gln Glu Thr Leu Ala Asn Ala His Ala Ala Lys Arg Trp Leu
210 215 220
ctg cag gcc ttc gac ggg gac gag tcg gcc gtc gcg aag cac ttc gtg 1020
Leu Gln Ala Phe Asp Gly Asp Glu Ser Ala Val Ala Lys His Phe Val
225 230 235 240
gcg gtg tcc acc aac gcc cag aag gtc gcc gag ttc ggc atc gac acc 1068
Ala Val Ser Thr Asn Ala Gln Lys Val Ala Glu Phe Gly Ile Asp Thr
245 250 255
gac aac atg ttc ggc ttc tgg gac tgg gta ggc ggc cgc tac tcc gtc 1116
Asp Asn Met Phe Gly Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Val
260 265 270
gac tcc gcc atc ggc ctg tcc ctc atg gcc gtc atc ggc ccg ctg gac 1164
Asp Ser Ala Ile Gly Leu Ser Leu Met Ala Val Ile Gly Pro Leu Asp
275 280 285
ttc atg cgc ttc ctc gag ggc ttc cgc gcc atg gac gag cac ttc cgc 1212
Phe Met Arg Phe Leu Glu Gly Phe Arg Ala Met Asp Glu His Phe Arg
290 295 300
gcg acc ccc ttc gag aag aac gtg ccc gtc ctc atg gcg ctg ctg ggc 1260
Ala Thr Pro Phe Glu Lys Asn Val Pro Val Leu Met Ala Leu Leu Gly
305 310 315 320
atc tgg tac gtc aac ttc cac ggc ttc gag acc cac gcg gtc ctc ccc 1308
Ile Trp Tyr Val Asn Phe His Gly Phe Glu Thr His Ala Val Leu Pro
325 330 335
tac tct cag gac ctc tcc cgt ttc gcc gcc tac ctg cag cag ctg acc 1356
Tyr Ser Gln Asp Leu Ser Arg Phe Ala Ala Tyr Leu Gln Gln Leu Thr
340 345 350
atg gag tcc aac ggc aag tcc gtc cga cac gac ggc acc gcc gtc gac 1404
Met Glu Ser Asn Gly Lys Ser Val Arg His Asp Gly Thr Ala Val Asp
355 360 365
tac gcc acc ggt gag atc tac tgg ggc gag ccg ggc acc aac ggc cag 1452
Tyr Ala Thr Gly Glu Ile Tyr Trp Gly Glu Pro Gly Thr Asn Gly Gln
370 375 380
cac gcc ttc tac cag ctc atg cac cag ggc acc cgc gtc gtc ccg gcc 1500
His Ala Phe Tyr Gln Leu Met His Gln Gly Thr Arg Val Val Pro Ala
385 390 395 400
gac ttc atc ggc ttc gcc cgc ccg aag aac gac ctg ccg acc gcc acc 1548
Asp Phe Ile Gly Phe Ala Arg Pro Lys Asn Asp Leu Pro Thr Ala Thr
405 410 415
ggc gag ggc tcc atg cac gac ctg ctc atg ggc aac ctc ttc gcc cag 1596
Gly Glu Gly Ser Met His Asp Leu Leu Met Gly Asn Leu Phe Ala Gln
420 425 430
agc aag gtg ctg gcc ttc ggc aag acc gcc gag gag atc gcc gcc gag 1644
Ser Lys Val Leu Ala Phe Gly Lys Thr Ala Glu Glu Ile Ala Ala Glu
435 440 445
gga gtg tcc gcg gac ctc gtc gcc cac aag gtc atg ccg ggc aac cgc 1692
Gly Val Ser Ala Asp Leu Val Ala His Lys Val Met Pro Gly Asn Arg
450 455 460
ccg tcc acg acc atc ctc gcg gag gag ctc acc ccg gcg gtg ctc ggc 1740
Pro Ser Thr Thr Ile Leu Ala Glu Glu Leu Thr Pro Ala Val Leu Gly
465 470 475 480
gcg ctc atc gcc ctc tac gag cac atc acc ttc gtc cag ggc gtc atc 1788
Ala Leu Ile Ala Leu Tyr Glu His Ile Thr Phe Val Gln Gly Val Ile
485 490 495
tgg gac atc aac tcc ttc gac cag tgg ggc gtg gag ctg ggc aag cag 1836
Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln
500 505 510
cag gcc aac cag ctg gcc gcc gcc gtc tcc ggc gcc gag gag gtc gac 1884
Gln Ala Asn Gln Leu Ala Ala Ala Val Ser Gly Ala Glu Glu Val Asp
515 520 525
tcc ggc gac tcc tcc acc gac gcg ctc gtg cgc tgg tac cgc gcg aac 1932
Ser Gly Asp Ser Ser Thr Asp Ala Leu Val Arg Trp Tyr Arg Ala Asn
530 535 540
cgc tag ttcggcaggg ccggcatccc gaggggggtg ccggccctct gcttctcgac 1988
Arg
545
gtcgccccgt tccccgtaac tgaaccggaa ctcgtggaac ggcaggttca cggtctccag 2048
gtggtagaag gcgttccggg cgtgcttgga gtagtggtac tcgcgggccc ggagggtggt 2108
caccgacagc aggttgccgt agccgggcag cagcaggtgc gcgtactgcc ggaggaaggg 2168
caccaggtcg aggctccatc cctcccccgt gcgggtcagt tccgccgggt acaggtcgac 2228
ggtggccacg 2238
<210> 6
<211> 545
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 6
Met Ala Asp Ile Thr Thr Ser Ala Glu Trp Lys Asn Leu Glu Ser Glu
1 5 10 15
Phe Glu Arg Thr Gln Gly Gln Thr Leu Arg Glu Leu Phe Ala Ala Asp
20 25 30
Pro Gln Arg Ala Glu Lys Leu Thr Phe Asp Ala Ala Gly Leu His Val
35 40 45
Asp Leu Ser Lys Asn Leu Leu Asp Asp Ala Thr Val Ala Ser Leu Val
50 55 60
Ala Leu Ala Lys Glu Ala Gln Leu Thr Glu Ala Ile Glu Ala Met Phe
65 70 75 80
Ser Gly Val His Ile Asn Asn Thr Glu Asp Arg Ala Val Leu His Thr
85 90 95
Ala Leu Arg Leu Pro Val Glu Glu Asp Leu Glu Val Asp Gly Gln Asp
100 105 110
Val Ala Ala Asp Val His Glu Val Leu Gly Arg Met Arg Asp Phe Ala
115 120 125
Ser Ser Leu Arg Ala Gly Thr Trp Leu Gly His Thr Gly Arg Thr Ile
130 135 140
Lys Lys Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala
145 150 155 160
Met Ala Ala Arg Ala Leu Arg Ser Tyr Ala Thr Ala Gly Ile Thr Ala
165 170 175
Glu Phe Val Ser Asn Val Asp Pro Ala Asp Met Ser Ala Thr Leu Asp
180 185 190
Glu Leu Asp Pro Glu Ser Thr Leu Phe Ile Ile Ala Ser Lys Thr Phe
195 200 205
Thr Thr Gln Glu Thr Leu Ala Asn Ala His Ala Ala Lys Arg Trp Leu
210 215 220
Leu Gln Ala Phe Asp Gly Asp Glu Ser Ala Val Ala Lys His Phe Val
225 230 235 240
Ala Val Ser Thr Asn Ala Gln Lys Val Ala Glu Phe Gly Ile Asp Thr
245 250 255
Asp Asn Met Phe Gly Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Val
260 265 270
Asp Ser Ala Ile Gly Leu Ser Leu Met Ala Val Ile Gly Pro Leu Asp
275 280 285
Phe Met Arg Phe Leu Glu Gly Phe Arg Ala Met Asp Glu His Phe Arg
290 295 300
Ala Thr Pro Phe Glu Lys Asn Val Pro Val Leu Met Ala Leu Leu Gly
305 310 315 320
Ile Trp Tyr Val Asn Phe His Gly Phe Glu Thr His Ala Val Leu Pro
325 330 335
Tyr Ser Gln Asp Leu Ser Arg Phe Ala Ala Tyr Leu Gln Gln Leu Thr
340 345 350
Met Glu Ser Asn Gly Lys Ser Val Arg His Asp Gly Thr Ala Val Asp
355 360 365
Tyr Ala Thr Gly Glu Ile Tyr Trp Gly Glu Pro Gly Thr Asn Gly Gln
370 375 380
His Ala Phe Tyr Gln Leu Met His Gln Gly Thr Arg Val Val Pro Ala
385 390 395 400
Asp Phe Ile Gly Phe Ala Arg Pro Lys Asn Asp Leu Pro Thr Ala Thr
405 410 415
Gly Glu Gly Ser Met His Asp Leu Leu Met Gly Asn Leu Phe Ala Gln
420 425 430
Ser Lys Val Leu Ala Phe Gly Lys Thr Ala Glu Glu Ile Ala Ala Glu
435 440 445
Gly Val Ser Ala Asp Leu Val Ala His Lys Val Met Pro Gly Asn Arg
450 455 460
Pro Ser Thr Thr Ile Leu Ala Glu Glu Leu Thr Pro Ala Val Leu Gly
465 470 475 480
Ala Leu Ile Ala Leu Tyr Glu His Ile Thr Phe Val Gln Gly Val Ile
485 490 495
Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln
500 505 510
Gln Ala Asn Gln Leu Ala Ala Ala Val Ser Gly Ala Glu Glu Val Asp
515 520 525
Ser Gly Asp Ser Ser Thr Asp Ala Leu Val Arg Trp Tyr Arg Ala Asn
530 535 540
Arg
545
<210> 7
<211> 2430
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2130)
<223> ген pck
<400> 7
cgctatgaca tctggactac agacccgcac cgcgcggcct ggggtctggc ggtcccggaa 60
cctgtgaaac ctcagtcaga ggggcccctg gcgtggggtt tagccggtat ggtcaccccc 120
gaaagtgtgg cctccaacca gggcatttcc ggggaaaagg cacggtggcg gcggatcgac 180
ggcggataaa cccgctggat atgacctgtg acccgtacct catgtcctgt gagaccgtca 240
caaccgcgat ttaatggata ccctgtatag agagaactgc cgcaaacgtc aggagaacac 300
atg acc act gcc atc aag ggc ctc gtc ggc ccc gca ccg acc gat aac 348
Met Thr Thr Ala Ile Lys Gly Leu Val Gly Pro Ala Pro Thr Asp Asn
1 5 10 15
gag aag ctc ctc acc tgg atc gcc gag gca gtg gag ctg atg cag ccg 396
Glu Lys Leu Leu Thr Trp Ile Ala Glu Ala Val Glu Leu Met Gln Pro
20 25 30
gag aag gtc gtc ttc gcc gac ggc tcc cag gag gag tgg gac cgc ctc 444
Glu Lys Val Val Phe Ala Asp Gly Ser Gln Glu Glu Trp Asp Arg Leu
35 40 45
tcc gcc gag ctc gtg gag gca ggc acc ttc atc cgc ctc aac gag gag 492
Ser Ala Glu Leu Val Glu Ala Gly Thr Phe Ile Arg Leu Asn Glu Glu
50 55 60
aag cgc ccg aac agc ttc ctg gcc cgc tcc aac ccg gac gat gtc gcc 540
Lys Arg Pro Asn Ser Phe Leu Ala Arg Ser Asn Pro Asp Asp Val Ala
65 70 75 80
cgc gtc gag tcc cgc acc ttc atc tgc tcc gag aag gag gag gac gcc 588
Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala
85 90 95
ggc ccg acc aac aac tgg gcg gac ccg gtc gag atg aag aag gag atg 636
Gly Pro Thr Asn Asn Trp Ala Asp Pro Val Glu Met Lys Lys Glu Met
100 105 110
acc gag gta tac cgc ggc tcc atg aag ggc cgc acc atg tac gtc gtg 684
Thr Glu Val Tyr Arg Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val
115 120 125
ccg ttc tgc atg ggc ccg atc acc gac ccg gag ccg aag ctc ggt gtc 732
Pro Phe Cys Met Gly Pro Ile Thr Asp Pro Glu Pro Lys Leu Gly Val
130 135 140
cag ctc acc gac tcc gcc tac gtc gtc ctc tcc atg cgc atc atg acc 780
Gln Leu Thr Asp Ser Ala Tyr Val Val Leu Ser Met Arg Ile Met Thr
145 150 155 160
cgt atg ggc tcc gag gcg ctc gag aag atc ggc acc gac ggc gat ttc 828
Arg Met Gly Ser Glu Ala Leu Glu Lys Ile Gly Thr Asp Gly Asp Phe
165 170 175
gtc cgc gca ctc cac tcc gtc ggc gcc ccg ctg gag ccg ggc cag gag 876
Val Arg Ala Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu
180 185 190
gac gtc atc tgg ccg tgc aac gac acc aag tac atc acc cag ttc ccg 924
Asp Val Ile Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro
195 200 205
gag acc aag gag atc tgg tcc tac ggc tcc ggt tac ggc ggc aac gcc 972
Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala
210 215 220
atc ctg gcc aag aag tgc tac gcc ctg cgc atc gcc tcc gtc atg gcc 1020
Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala
225 230 235 240
aag gag gag ggc tgg atg gct gag cac atg ctc atc ctg aag ctc aac 1068
Lys Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Asn
245 250 255
tcc ccg gag ggc aag agc tac cac atc gcg gca gct ttc ccg tcc gcc 1116
Ser Pro Glu Gly Lys Ser Tyr His Ile Ala Ala Ala Phe Pro Ser Ala
260 265 270
tgc ggc aag acc aac ctc gcc atg atc acc ccg acc atc gag ggc tgg 1164
Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Glu Gly Trp
275 280 285
acc gcc gag gtc gtc ggc gac gac atc gcc tgg ctc aag ctg cgc gag 1212
Thr Ala Glu Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu
290 295 300
gac ggc ctc tac gcc gtc aac ccg gag aac ggc ttc ttc ggc gtc gca 1260
Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala
305 310 315 320
ccg ggc acc aac tac gcc tcc aac ccg atc gcc atg gag acc atg gag 1308
Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Glu Thr Met Glu
325 330 335
ccg ggc aac acc ctg ttc acc aac gtc gca ctg acc gac gac ggt gac 1356
Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp
340 345 350
gtc tgg tgg gag ggc atg gac ggc gag aag ccg gag cac ctc atc gac 1404
Val Trp Trp Glu Gly Met Asp Gly Glu Lys Pro Glu His Leu Ile Asp
355 360 365
tgg ctg ggc aac gac tgg acc ccg gag tcc ggc acc aac gcc gca cac 1452
Trp Leu Gly Asn Asp Trp Thr Pro Glu Ser Gly Thr Asn Ala Ala His
370 375 380
ccg aac tcc cgc tac tgc gtg gcc atc gag cag tgc ccg gtc gcc gca 1500
Pro Asn Ser Arg Tyr Cys Val Ala Ile Glu Gln Cys Pro Val Ala Ala
385 390 395 400
ccg gag ttc aac gac ccg aag ggc gtc aag atc gac gcc atc ctc ttc 1548
Pro Glu Phe Asn Asp Pro Lys Gly Val Lys Ile Asp Ala Ile Leu Phe
405 410 415
ggt ggc cgt cgt gcc gac acc gtc ccg ctg gtc acc cag tcc tac gac 1596
Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Ser Tyr Asp
420 425 430
tgg gag cac ggc acc ttc atc ggt gcg acc ctg gcc tcc ggc cag acc 1644
Trp Glu His Gly Thr Phe Ile Gly Ala Thr Leu Ala Ser Gly Gln Thr
435 440 445
gcc gca tcc gct gag gcg aag gtc ggc tcc ctg cgc cac gac ccg atg 1692
Ala Ala Ser Ala Glu Ala Lys Val Gly Ser Leu Arg His Asp Pro Met
450 455 460
gcc atg ctg ccg ttc atg ggc tac aac gcc ggt gac tac ctg cag cac 1740
Ala Met Leu Pro Phe Met Gly Tyr Asn Ala Gly Asp Tyr Leu Gln His
465 470 475 480
tgg atc gac atg ggc aag aag ggc ggc gac cgt atg ccg gcc atc ttc 1788
Trp Ile Asp Met Gly Lys Lys Gly Gly Asp Arg Met Pro Ala Ile Phe
485 490 495
ctg gtc aac tgg ttc cgc cgt ggc gag gac ggc cgc ttc ctg tgg ccg 1836
Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro
500 505 510
ggc ttc ggt gac aac tcc cgt gtc ctg aag tgg atc att gac cgc atc 1884
Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Ile Ile Asp Arg Ile
515 520 525
gag ggc aag gtc gac gcc gag gag acc gtc gtg ggc cac acc gcc cgc 1932
Glu Gly Lys Val Asp Ala Glu Glu Thr Val Val Gly His Thr Ala Arg
530 535 540
gcc gag gac ctc gac ctg acc ggc ctg gac acc ccg ctc gag gac gtc 1980
Ala Glu Asp Leu Asp Leu Thr Gly Leu Asp Thr Pro Leu Glu Asp Val
545 550 555 560
aag gag gcc ctg act gcg ccg gcc gca gac tgg gag cgc gac gtc gcc 2028
Lys Glu Ala Leu Thr Ala Pro Ala Ala Asp Trp Glu Arg Asp Val Ala
565 570 575
gac aac gag gag tac ctg gca ttc ctc ggc ccg cgt gtc ccg aac gag 2076
Asp Asn Glu Glu Tyr Leu Ala Phe Leu Gly Pro Arg Val Pro Asn Glu
580 585 590
gtc cac gag cag ctg ggc aag ctc aag gag cgc atc gag gcg aac aag 2124
Val His Glu Gln Leu Gly Lys Leu Lys Glu Arg Ile Glu Ala Asn Lys
595 600 605
aac tag ttcgtctccc ctcagaactt ccaccctcca ggttgactca gtccctgggg 2180
Asn
ggtgggattt tttctgcccc ggtcgcaccg taagtccgag agaggatgtt cgagaggaga 2240
gtcctctcga acgtgcggga cccccgcggg actcctctcg acgatgtgat ctcgacgtca 2300
gggaagaggg gggaagggga acgccgtcag tgcttccgca gcacgaccac caggttcgac 2360
acgaggaact cccgcagcag cggcacccgc accatccacc acgcccacga ggggtggtag 2420
cgggggaagg 2430
<210> 8
<211> 609
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 8
Met Thr Thr Ala Ile Lys Gly Leu Val Gly Pro Ala Pro Thr Asp Asn
1 5 10 15
Glu Lys Leu Leu Thr Trp Ile Ala Glu Ala Val Glu Leu Met Gln Pro
20 25 30
Glu Lys Val Val Phe Ala Asp Gly Ser Gln Glu Glu Trp Asp Arg Leu
35 40 45
Ser Ala Glu Leu Val Glu Ala Gly Thr Phe Ile Arg Leu Asn Glu Glu
50 55 60
Lys Arg Pro Asn Ser Phe Leu Ala Arg Ser Asn Pro Asp Asp Val Ala
65 70 75 80
Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala
85 90 95
Gly Pro Thr Asn Asn Trp Ala Asp Pro Val Glu Met Lys Lys Glu Met
100 105 110
Thr Glu Val Tyr Arg Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val
115 120 125
Pro Phe Cys Met Gly Pro Ile Thr Asp Pro Glu Pro Lys Leu Gly Val
130 135 140
Gln Leu Thr Asp Ser Ala Tyr Val Val Leu Ser Met Arg Ile Met Thr
145 150 155 160
Arg Met Gly Ser Glu Ala Leu Glu Lys Ile Gly Thr Asp Gly Asp Phe
165 170 175
Val Arg Ala Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu
180 185 190
Asp Val Ile Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro
195 200 205
Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala
210 215 220
Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala
225 230 235 240
Lys Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Asn
245 250 255
Ser Pro Glu Gly Lys Ser Tyr His Ile Ala Ala Ala Phe Pro Ser Ala
260 265 270
Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Glu Gly Trp
275 280 285
Thr Ala Glu Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu
290 295 300
Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala
305 310 315 320
Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Glu Thr Met Glu
325 330 335
Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp
340 345 350
Val Trp Trp Glu Gly Met Asp Gly Glu Lys Pro Glu His Leu Ile Asp
355 360 365
Trp Leu Gly Asn Asp Trp Thr Pro Glu Ser Gly Thr Asn Ala Ala His
370 375 380
Pro Asn Ser Arg Tyr Cys Val Ala Ile Glu Gln Cys Pro Val Ala Ala
385 390 395 400
Pro Glu Phe Asn Asp Pro Lys Gly Val Lys Ile Asp Ala Ile Leu Phe
405 410 415
Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Ser Tyr Asp
420 425 430
Trp Glu His Gly Thr Phe Ile Gly Ala Thr Leu Ala Ser Gly Gln Thr
435 440 445
Ala Ala Ser Ala Glu Ala Lys Val Gly Ser Leu Arg His Asp Pro Met
450 455 460
Ala Met Leu Pro Phe Met Gly Tyr Asn Ala Gly Asp Tyr Leu Gln His
465 470 475 480
Trp Ile Asp Met Gly Lys Lys Gly Gly Asp Arg Met Pro Ala Ile Phe
485 490 495
Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro
500 505 510
Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Ile Ile Asp Arg Ile
515 520 525
Glu Gly Lys Val Asp Ala Glu Glu Thr Val Val Gly His Thr Ala Arg
530 535 540
Ala Glu Asp Leu Asp Leu Thr Gly Leu Asp Thr Pro Leu Glu Asp Val
545 550 555 560
Lys Glu Ala Leu Thr Ala Pro Ala Ala Asp Trp Glu Arg Asp Val Ala
565 570 575
Asp Asn Glu Glu Tyr Leu Ala Phe Leu Gly Pro Arg Val Pro Asn Glu
580 585 590
Val His Glu Gln Leu Gly Lys Leu Lys Glu Arg Ile Glu Ala Asn Lys
595 600 605
Asn
<210> 9
<211> 1461
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1161)
<223> ген metQ
<400> 9
aagctgtagg agctcacggc gtggagctcc gcgaagggga gggcggaggg ggcgacgggg 60
cggcgtcggc aagcaccctc cccgagatgg ccgaccggga cgggcgtggg gcccgggcgg 120
ccggagagga tgcgctccag ccgcgaccag ccgaggtgtg ccccaccgtg gaatcccatg 180
ccccgcaatc tacccctgat cgaacagatg tgccacactg cccgcggcaa ggactagaca 240
gagcggtacg cccggtgcta ggtttcagaa ccaacgaacc gaacacgtaa ggacaccacc 300
atg act ttc cgt cgc ctc ctg gcc ggc acc gcc gcc gcc gtc atc gcc 348
Met Thr Phe Arg Arg Leu Leu Ala Gly Thr Ala Ala Ala Val Ile Ala
1 5 10 15
tcc gtg ggc ctc gtc gcc tgc ggc tcc ggc tcc tcc ggg gag acc gtc 396
Ser Val Gly Leu Val Ala Cys Gly Ser Gly Ser Ser Gly Glu Thr Val
20 25 30
cgc atc ggc acc acc gac gcc cag ctc aag gag tgg gac gtc ttc gcc 444
Arg Ile Gly Thr Thr Asp Ala Gln Leu Lys Glu Trp Asp Val Phe Ala
35 40 45
gac atc gtc gag gcg gag ggc atc gac atc gag atc gtg ccg ttc tcc 492
Asp Ile Val Glu Ala Glu Gly Ile Asp Ile Glu Ile Val Pro Phe Ser
50 55 60
gac tac aac acc ccg aac gac gcg ctc gtg cag ggt gag atc gac gtc 540
Asp Tyr Asn Thr Pro Asn Asp Ala Leu Val Gln Gly Glu Ile Asp Val
65 70 75 80
aac aag ttc cag cac ctg gac ttc ctc gcc tcc tac aac aaa ggc gcc 588
Asn Lys Phe Gln His Leu Asp Phe Leu Ala Ser Tyr Asn Lys Gly Ala
85 90 95
aac gcc gac ctc gtg ccg tac gcc gcc acc gag atc aac ccg ctg tcc 636
Asn Ala Asp Leu Val Pro Tyr Ala Ala Thr Glu Ile Asn Pro Leu Ser
100 105 110
ctg ttc tgg aag gac cac gac tcc ctc gac ggc atc gag ggc gag ggc 684
Leu Phe Trp Lys Asp His Asp Ser Leu Asp Gly Ile Glu Gly Glu Gly
115 120 125
gtc gtc atc ccc aac gac gac acc aac cag ggc cgc gcc atc aac gtc 732
Val Val Ile Pro Asn Asp Asp Thr Asn Gln Gly Arg Ala Ile Asn Val
130 135 140
ctc gcc cag gcc ggt ctg gtg acc ctg cgc gac gac agc gtc tac aac 780
Leu Ala Gln Ala Gly Leu Val Thr Leu Arg Asp Asp Ser Val Tyr Asn
145 150 155 160
ccg acc ccg gcc gac gtc gac acc gag aag tcc aag gtc tcc atc gtc 828
Pro Thr Pro Ala Asp Val Asp Thr Glu Lys Ser Lys Val Ser Ile Val
165 170 175
acc gtc gac gcc gcc cag acc acc acc gcc tac cac gag ggt cgc ccg 876
Thr Val Asp Ala Ala Gln Thr Thr Thr Ala Tyr His Glu Gly Arg Pro
180 185 190
gcc atc atc aac aac tcc ttc ctg gag cgc gcc agc atc gac ccg tcc 924
Ala Ile Ile Asn Asn Ser Phe Leu Glu Arg Ala Ser Ile Asp Pro Ser
195 200 205
ctc gcc atc ttc cag gat gac ccg agc acc gag cag gcc gag ccg tac 972
Leu Ala Ile Phe Gln Asp Asp Pro Ser Thr Glu Gln Ala Glu Pro Tyr
210 215 220
atc aac gcc tgg gtg acc acc cgc gac aag gcc gac gac gag acc ctc 1020
Ile Asn Ala Trp Val Thr Thr Arg Asp Lys Ala Asp Asp Glu Thr Leu
225 230 235 240
atc cgt ctc gcc gag ctg tgg aag gac ccg gcc gtc acc gcc gcc gtc 1068
Ile Arg Leu Ala Glu Leu Trp Lys Asp Pro Ala Val Thr Ala Ala Val
245 250 255
ctg gag acc tcc ggc aac acc gcc gtc acc gtc gag cgc tcg ccc gag 1116
Leu Glu Thr Ser Gly Asn Thr Ala Val Thr Val Glu Arg Ser Pro Glu
260 265 270
gag ctc gcc gcg atc atg gag cgc ctc gcg gcc cgc gag cag tag 1161
Glu Leu Ala Ala Ile Met Glu Arg Leu Ala Ala Arg Glu Gln
275 280 285
gccgcactcc gcccacctgc cccgtcgcgt tcccgcggcg gggtttagca tttacctgac 1221
cagtccccct cccagaacgg cccccagaac accgtgaccg aacctgtgac cgcaccaccc 1281
acccggccgc gtaccggtac ccgcatcgag ttccgcgatg tgaccaaggt cttcaccaac 1341
cagcggaaga ccgagacccg cgccgtcgac ggcgtcaccc tgaccgtcga acccggcgag 1401
atcctcggcg tcatcggcta ctccggcgcc ggcaagtcga cgctcgtccg cctcatcaac 1461
<210> 10
<211> 286
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 10
Met Thr Phe Arg Arg Leu Leu Ala Gly Thr Ala Ala Ala Val Ile Ala
1 5 10 15
Ser Val Gly Leu Val Ala Cys Gly Ser Gly Ser Ser Gly Glu Thr Val
20 25 30
Arg Ile Gly Thr Thr Asp Ala Gln Leu Lys Glu Trp Asp Val Phe Ala
35 40 45
Asp Ile Val Glu Ala Glu Gly Ile Asp Ile Glu Ile Val Pro Phe Ser
50 55 60
Asp Tyr Asn Thr Pro Asn Asp Ala Leu Val Gln Gly Glu Ile Asp Val
65 70 75 80
Asn Lys Phe Gln His Leu Asp Phe Leu Ala Ser Tyr Asn Lys Gly Ala
85 90 95
Asn Ala Asp Leu Val Pro Tyr Ala Ala Thr Glu Ile Asn Pro Leu Ser
100 105 110
Leu Phe Trp Lys Asp His Asp Ser Leu Asp Gly Ile Glu Gly Glu Gly
115 120 125
Val Val Ile Pro Asn Asp Asp Thr Asn Gln Gly Arg Ala Ile Asn Val
130 135 140
Leu Ala Gln Ala Gly Leu Val Thr Leu Arg Asp Asp Ser Val Tyr Asn
145 150 155 160
Pro Thr Pro Ala Asp Val Asp Thr Glu Lys Ser Lys Val Ser Ile Val
165 170 175
Thr Val Asp Ala Ala Gln Thr Thr Thr Ala Tyr His Glu Gly Arg Pro
180 185 190
Ala Ile Ile Asn Asn Ser Phe Leu Glu Arg Ala Ser Ile Asp Pro Ser
195 200 205
Leu Ala Ile Phe Gln Asp Asp Pro Ser Thr Glu Gln Ala Glu Pro Tyr
210 215 220
Ile Asn Ala Trp Val Thr Thr Arg Asp Lys Ala Asp Asp Glu Thr Leu
225 230 235 240
Ile Arg Leu Ala Glu Leu Trp Lys Asp Pro Ala Val Thr Ala Ala Val
245 250 255
Leu Glu Thr Ser Gly Asn Thr Ala Val Thr Val Glu Arg Ser Pro Glu
260 265 270
Glu Leu Ala Ala Ile Met Glu Arg Leu Ala Ala Arg Glu Gln
275 280 285
<210> 11
<211> 2259
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1959)
<223> ген metP
<400> 11
tcaatcgtcg cgaaggggta gttcgcggcg agaacatcgt ttcgggtcag ggcattgaag 60
agggtggact tgccgacgtt gggcagaccg acgattccaa gagtaaggct cacgggcgta 120
gatcctagca gccagcggtt ttgcctctcc ggtcagcgcc cgtacctgtg ttgcgggatg 180
atacggacgt cattatatga tcaggccact tcaacgataa atacgtcgca ccctggacgt 240
tttcctctcc ctaaccatcg ggagccaact acacatcctc cacctcagac aggtatacac 300
atg tct tcg acc acc ccg cag ccc gcg ccg tcg ggc cag gag cag ggc 348
Met Ser Ser Thr Thr Pro Gln Pro Ala Pro Ser Gly Gln Glu Gln Gly
1 5 10 15
gag cgg cgc gag atc ttc tcg tcc cgt tgg gcg ttc ctg ctg gcc gcc 396
Glu Arg Arg Glu Ile Phe Ser Ser Arg Trp Ala Phe Leu Leu Ala Ala
20 25 30
atc ggc tcc gcc gtc ggc ctg ggc aac atc tgg cgc ttc ccc tac gtc 444
Ile Gly Ser Ala Val Gly Leu Gly Asn Ile Trp Arg Phe Pro Tyr Val
35 40 45
gct tac gac aac ggc ggc ggc gcc ttc ctc atc ccc tac ctc gtg gca 492
Ala Tyr Asp Asn Gly Gly Gly Ala Phe Leu Ile Pro Tyr Leu Val Ala
50 55 60
ctg ctg tcc gct ggt atc ccg ctg ctc ttc ctc gac tac gcc ctg ggc 540
Leu Leu Ser Ala Gly Ile Pro Leu Leu Phe Leu Asp Tyr Ala Leu Gly
65 70 75 80
cac cgc tac cgt ggt tcc gcc ccg ctg gtg ttc cgt cgc atc aag tcc 588
His Arg Tyr Arg Gly Ser Ala Pro Leu Val Phe Arg Arg Ile Lys Ser
85 90 95
tgg gct gag ccg gtc ggc tgg gtc cag gtc ggt gtg tcc ttt ttc atc 636
Trp Ala Glu Pro Val Gly Trp Val Gln Val Gly Val Ser Phe Phe Ile
100 105 110
act atc tac tac gcc gcg atc atc ggc tgg gcc gcg ctc tac gcg gtc 684
Thr Ile Tyr Tyr Ala Ala Ile Ile Gly Trp Ala Ala Leu Tyr Ala Val
115 120 125
aag tcc ttc aac aag gcg tgg ggc acc gac ccg aac agc tac ttc ttc 732
Lys Ser Phe Asn Lys Ala Trp Gly Thr Asp Pro Asn Ser Tyr Phe Phe
130 135 140
ggc gat ttc ctc cag ttc gac gag acc gcc acc ttc tcc acc gac ttc 780
Gly Asp Phe Leu Gln Phe Asp Glu Thr Ala Thr Phe Ser Thr Asp Phe
145 150 155 160
gtc ccg cag atc gcc ctg gcc ctg gcc ggc gtg tgg atc gcc gcg atc 828
Val Pro Gln Ile Ala Leu Ala Leu Ala Gly Val Trp Ile Ala Ala Ile
165 170 175
atc gtg ctg gcg atc ggc gtg gac aag ggc atc ggc aag gtc tcc aag 876
Ile Val Leu Ala Ile Gly Val Asp Lys Gly Ile Gly Lys Val Ser Lys
180 185 190
atc ttc atc ccc ctg ctg gcg gtc ctc ttc ctc atc gtc gta gtc cag 924
Ile Phe Ile Pro Leu Leu Ala Val Leu Phe Leu Ile Val Val Val Gln
195 200 205
gcg ctc atg ctg ccg ggt gcc gcc acg ggt ctc gac gcc ctg ttc acc 972
Ala Leu Met Leu Pro Gly Ala Ala Thr Gly Leu Asp Ala Leu Phe Thr
210 215 220
ccg gac tgg aac gcc ctg acc aac ggc act gtg tgg atc gcc gcc tac 1020
Pro Asp Trp Asn Ala Leu Thr Asn Gly Thr Val Trp Ile Ala Ala Tyr
225 230 235 240
ggc cag atc ttc ttc tcc ctg tcc gtg agc ttc ggc atc atg ctc acc 1068
Gly Gln Ile Phe Phe Ser Leu Ser Val Ser Phe Gly Ile Met Leu Thr
245 250 255
tac tcc tcc tac ctg cgt ccg cgc acc aac ctg acg ggc acc ggc ctg 1116
Tyr Ser Ser Tyr Leu Arg Pro Arg Thr Asn Leu Thr Gly Thr Gly Leu
260 265 270
acc acc gcc ttc gcg aac tcc tcc ttc gag gtc ctc gcc ggc gtc ggt 1164
Thr Thr Ala Phe Ala Asn Ser Ser Phe Glu Val Leu Ala Gly Val Gly
275 280 285
gtc ttc gcc gcc ctg ggc ttc atg gcc gtg cag cag tcg gtc gcc gtc 1212
Val Phe Ala Ala Leu Gly Phe Met Ala Val Gln Gln Ser Val Ala Val
290 295 300
gac gag gtc gcg acc tcg ggc atc ggt ctg gcc ttc gtg gcc ttc ccg 1260
Asp Glu Val Ala Thr Ser Gly Ile Gly Leu Ala Phe Val Ala Phe Pro
305 310 315 320
gcg atc atc aac gag atg gcc ttc ggc ccg atc ttc ggc gtc ctg ttc 1308
Ala Ile Ile Asn Glu Met Ala Phe Gly Pro Ile Phe Gly Val Leu Phe
325 330 335
ttc ggc tcc ctc acc atc gcc ggc ttc acc tcg ctg ttc tcc ctg ctc 1356
Phe Gly Ser Leu Thr Ile Ala Gly Phe Thr Ser Leu Phe Ser Leu Leu
340 345 350
gag gtc gtc gtc tcc gcc gtc aag gac aag ctg aac ctg ccg cgc aag 1404
Glu Val Val Val Ser Ala Val Lys Asp Lys Leu Asn Leu Pro Arg Lys
355 360 365
acc gtc gcc atc ggc atc ggc acc ttc atg gcg ctg atc tcc ctg gcg 1452
Thr Val Ala Ile Gly Ile Gly Thr Phe Met Ala Leu Ile Ser Leu Ala
370 375 380
ctg ttc tcc acg acg tcc ggc ctc atg gcc ctg gac atc atg gac aag 1500
Leu Phe Ser Thr Thr Ser Gly Leu Met Ala Leu Asp Ile Met Asp Lys
385 390 395 400
ttc acc aac aac atc ggc atc gtc gcc atc gcc ctg atc gcg gtc atc 1548
Phe Thr Asn Asn Ile Gly Ile Val Ala Ile Ala Leu Ile Ala Val Ile
405 410 415
atc ctc gat tgg gtc ctg cgc cgg atc gac gag ttc tcg ctc cac atc 1596
Ile Leu Asp Trp Val Leu Arg Arg Ile Asp Glu Phe Ser Leu His Ile
420 425 430
aac gcc gtg tcc tcc ttc aag gtg ggc acg ctg tgg cgc atc ttc gtg 1644
Asn Ala Val Ser Ser Phe Lys Val Gly Thr Leu Trp Arg Ile Phe Val
435 440 445
gtc aac gtc acc acc ctg gtg ctg gga ttc acc ctg gcg cag gag ctc 1692
Val Asn Val Thr Thr Leu Val Leu Gly Phe Thr Leu Ala Gln Glu Leu
450 455 460
atc tcc ctg atc cag gag ccc tac ggc ggc tac acg acg acg cag atc 1740
Ile Ser Leu Ile Gln Glu Pro Tyr Gly Gly Tyr Thr Thr Thr Gln Ile
465 470 475 480
gcc atc tac ggc tgg gcc gtc ctg gcg ctc atc ggt atc aac gcc ctc 1788
Ala Ile Tyr Gly Trp Ala Val Leu Ala Leu Ile Gly Ile Asn Ala Leu
485 490 495
gtc ttc tcc ctg gtg ccg tgg cgc ggc aac ctg ccg ctc aac ggt ccg 1836
Val Phe Ser Leu Val Pro Trp Arg Gly Asn Leu Pro Leu Asn Gly Pro
500 505 510
ccg ggc tcc gac ttc ggt gtt gac ccg gag gtc cac cgc gag ctc cac 1884
Pro Gly Ser Asp Phe Gly Val Asp Pro Glu Val His Arg Glu Leu His
515 520 525
gcg ccg cgc aag tac aac ggt gac cac cgg gtg ggc gtc gac aag cac 1932
Ala Pro Arg Lys Tyr Asn Gly Asp His Arg Val Gly Val Asp Lys His
530 535 540
acc acc gag gaa gga acg atc cga tga gcggtctcgc aatcatgatg 1979
Thr Thr Glu Glu Gly Thr Ile Arg
545 550
atggtcctgt tcatggtcgt catctggggt ggtttcatcg ccagcgccct gcacctgcgg 2039
gcgaaccccg atgacacctc cggcgccctg ggcgtcgcgg accatgcgcg cgacgagcac 2099
ctcgccgcgc aggaactcca ctagtcacac cacaccggga tgtcaggcaa tatggagtct 2159
gtgactgacc agccgacctc ccgcatcccc ggggacgaga ctcccccgac cggctcctcc 2219
ggcgacgccg ggtccaccgc cttcggcagc atggacctgg 2259
<210> 12
<211> 552
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 12
Met Ser Ser Thr Thr Pro Gln Pro Ala Pro Ser Gly Gln Glu Gln Gly
1 5 10 15
Glu Arg Arg Glu Ile Phe Ser Ser Arg Trp Ala Phe Leu Leu Ala Ala
20 25 30
Ile Gly Ser Ala Val Gly Leu Gly Asn Ile Trp Arg Phe Pro Tyr Val
35 40 45
Ala Tyr Asp Asn Gly Gly Gly Ala Phe Leu Ile Pro Tyr Leu Val Ala
50 55 60
Leu Leu Ser Ala Gly Ile Pro Leu Leu Phe Leu Asp Tyr Ala Leu Gly
65 70 75 80
His Arg Tyr Arg Gly Ser Ala Pro Leu Val Phe Arg Arg Ile Lys Ser
85 90 95
Trp Ala Glu Pro Val Gly Trp Val Gln Val Gly Val Ser Phe Phe Ile
100 105 110
Thr Ile Tyr Tyr Ala Ala Ile Ile Gly Trp Ala Ala Leu Tyr Ala Val
115 120 125
Lys Ser Phe Asn Lys Ala Trp Gly Thr Asp Pro Asn Ser Tyr Phe Phe
130 135 140
Gly Asp Phe Leu Gln Phe Asp Glu Thr Ala Thr Phe Ser Thr Asp Phe
145 150 155 160
Val Pro Gln Ile Ala Leu Ala Leu Ala Gly Val Trp Ile Ala Ala Ile
165 170 175
Ile Val Leu Ala Ile Gly Val Asp Lys Gly Ile Gly Lys Val Ser Lys
180 185 190
Ile Phe Ile Pro Leu Leu Ala Val Leu Phe Leu Ile Val Val Val Gln
195 200 205
Ala Leu Met Leu Pro Gly Ala Ala Thr Gly Leu Asp Ala Leu Phe Thr
210 215 220
Pro Asp Trp Asn Ala Leu Thr Asn Gly Thr Val Trp Ile Ala Ala Tyr
225 230 235 240
Gly Gln Ile Phe Phe Ser Leu Ser Val Ser Phe Gly Ile Met Leu Thr
245 250 255
Tyr Ser Ser Tyr Leu Arg Pro Arg Thr Asn Leu Thr Gly Thr Gly Leu
260 265 270
Thr Thr Ala Phe Ala Asn Ser Ser Phe Glu Val Leu Ala Gly Val Gly
275 280 285
Val Phe Ala Ala Leu Gly Phe Met Ala Val Gln Gln Ser Val Ala Val
290 295 300
Asp Glu Val Ala Thr Ser Gly Ile Gly Leu Ala Phe Val Ala Phe Pro
305 310 315 320
Ala Ile Ile Asn Glu Met Ala Phe Gly Pro Ile Phe Gly Val Leu Phe
325 330 335
Phe Gly Ser Leu Thr Ile Ala Gly Phe Thr Ser Leu Phe Ser Leu Leu
340 345 350
Glu Val Val Val Ser Ala Val Lys Asp Lys Leu Asn Leu Pro Arg Lys
355 360 365
Thr Val Ala Ile Gly Ile Gly Thr Phe Met Ala Leu Ile Ser Leu Ala
370 375 380
Leu Phe Ser Thr Thr Ser Gly Leu Met Ala Leu Asp Ile Met Asp Lys
385 390 395 400
Phe Thr Asn Asn Ile Gly Ile Val Ala Ile Ala Leu Ile Ala Val Ile
405 410 415
Ile Leu Asp Trp Val Leu Arg Arg Ile Asp Glu Phe Ser Leu His Ile
420 425 430
Asn Ala Val Ser Ser Phe Lys Val Gly Thr Leu Trp Arg Ile Phe Val
435 440 445
Val Asn Val Thr Thr Leu Val Leu Gly Phe Thr Leu Ala Gln Glu Leu
450 455 460
Ile Ser Leu Ile Gln Glu Pro Tyr Gly Gly Tyr Thr Thr Thr Gln Ile
465 470 475 480
Ala Ile Tyr Gly Trp Ala Val Leu Ala Leu Ile Gly Ile Asn Ala Leu
485 490 495
Val Phe Ser Leu Val Pro Trp Arg Gly Asn Leu Pro Leu Asn Gly Pro
500 505 510
Pro Gly Ser Asp Phe Gly Val Asp Pro Glu Val His Arg Glu Leu His
515 520 525
Ala Pro Arg Lys Tyr Asn Gly Asp His Arg Val Gly Val Asp Lys His
530 535 540
Thr Thr Glu Glu Gly Thr Ile Arg
545 550
<210> 13
<211> 1650
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1350)
<223> ген metN
<400> 13
ccgtacatca acgcctgggt gaccacccgc gacaaggccg acgacgagac cctcatccgt 60
ctcgccgagc tgtggaagga cccggccgtc accgccgccg tcctggagac ctccggcaac 120
accgccgtca ccgtcgagcg ctcgcccgag gagctcgccg cgatcatgga gcgcctcgcg 180
gcccgcgagc agtaggccgc actccgccca cctgccccgt cgcgttcccg cggcggggtt 240
tagcatttac ctgaccagtc cccctcccag aacggccccc agaacaccgt gaccgaacct 300
gtg acc gca cca ccc acc cgg ccg cgt acc ggt acc cgc atc gag ttc 348
Val Thr Ala Pro Pro Thr Arg Pro Arg Thr Gly Thr Arg Ile Glu Phe
1 5 10 15
cgc gat gtg acc aag gtc ttc acc aac cag cgg aag acc gag acc cgc 396
Arg Asp Val Thr Lys Val Phe Thr Asn Gln Arg Lys Thr Glu Thr Arg
20 25 30
gcc gtc gac ggc gtc acc ctg acc gtc gaa ccc ggc gag atc ctc ggc 444
Ala Val Asp Gly Val Thr Leu Thr Val Glu Pro Gly Glu Ile Leu Gly
35 40 45
gtc atc ggc tac tcc ggc gcc ggc aag tcg acg ctc gtc cgc ctc atc 492
Val Ile Gly Tyr Ser Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile
50 55 60
aac ggg ctg gac tcc aca acc tcc ggc cag ctg ctt ctc gac ggc acc 540
Asn Gly Leu Asp Ser Thr Thr Ser Gly Gln Leu Leu Leu Asp Gly Thr
65 70 75 80
gac atc gtc gga ctc ccc gag tcc aag ctg cgc tcg atc cgc cgg ggc 588
Asp Ile Val Gly Leu Pro Glu Ser Lys Leu Arg Ser Ile Arg Arg Gly
85 90 95
atc ggc atg atc ttc cag cag ttc aac ctc ttc cag tcc cgc acg gcc 636
Ile Gly Met Ile Phe Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala
100 105 110
gcc ggc aac atc gcc tac ccg ctg gag ctg gcc ggc atg ccg cgc gcg 684
Ala Gly Asn Ile Ala Tyr Pro Leu Glu Leu Ala Gly Met Pro Arg Ala
115 120 125
gag cgg cgg cgg cgc gtc gcc gag ctc ctc gac ttc gtc ggc ctg ggc 732
Glu Arg Arg Arg Arg Val Ala Glu Leu Leu Asp Phe Val Gly Leu Gly
130 135 140
gac aag ggc gac aac tac ccg gag cag ctg tcg ggc ggc cag aag cag 780
Asp Lys Gly Asp Asn Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln
145 150 155 160
cgc gtc ggc atc gcc cgc gcc ctg gcc acc aac ccg cgg ctc ctg ctc 828
Arg Val Gly Ile Ala Arg Ala Leu Ala Thr Asn Pro Arg Leu Leu Leu
165 170 175
gcc gac gag gcc acc tcg gcc ctc gac ccg gag acc acc cag gac gtg 876
Ala Asp Glu Ala Thr Ser Ala Leu Asp Pro Glu Thr Thr Gln Asp Val
180 185 190
ctc gac ctg ctc cgg gag atc aac cgc gag ctg ggc atc acc atc gtc 924
Leu Asp Leu Leu Arg Glu Ile Asn Arg Glu Leu Gly Ile Thr Ile Val
195 200 205
gta atc acc cat gag atg gac gtc gtg cgt tcc atc gcc gac aag gtc 972
Val Ile Thr His Glu Met Asp Val Val Arg Ser Ile Ala Asp Lys Val
210 215 220
gcc gtc atg gag gcc ggc cgg gtg gtc gag cac ggc agc gtc tac gag 1020
Ala Val Met Glu Ala Gly Arg Val Val Glu His Gly Ser Val Tyr Glu
225 230 235 240
gtg ttc tcc cgc ccg cag act gcg gtg gcg cgg cgc ttc gtg tcc acc 1068
Val Phe Ser Arg Pro Gln Thr Ala Val Ala Arg Arg Phe Val Ser Thr
245 250 255
tcg ctg cgc aac acc ccc gac cag gtc gag gcg gag gac ctg ctg gcc 1116
Ser Leu Arg Asn Thr Pro Asp Gln Val Glu Ala Glu Asp Leu Leu Ala
260 265 270
cac gag ggc cgc ctg ttc acc atc acc ctc acg gag gac tcc ggg ttc 1164
His Glu Gly Arg Leu Phe Thr Ile Thr Leu Thr Glu Asp Ser Gly Phe
275 280 285
ttc gcc gcg gcc gcc gcc ggc cgt gag gag ggg atg ctc atc aac atc 1212
Phe Ala Ala Ala Ala Ala Gly Arg Glu Glu Gly Met Leu Ile Asn Ile
290 295 300
gtc cac ggc ggc gtc acc acg ctg cag cgg cag tcc ttc ggc aag atg 1260
Val His Gly Gly Val Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met
305 310 315 320
acc gtc cgg gtg acg ggg ccg gac gcg gcc gtc gag aag ttc cgt gac 1308
Thr Val Arg Val Thr Gly Pro Asp Ala Ala Val Glu Lys Phe Arg Asp
325 330 335
acg ctg tcc cgc acc acc gac atc cag gag atc acg cga tga 1350
Thr Leu Ser Arg Thr Thr Asp Ile Gln Glu Ile Thr Arg
340 345
acgagaccct cctcgccgca gactggaacc gtctcggccc gaccttcatc gacgccatcg 1410
gcgacaccct cgtcatggtg tccctcacga tggtgctcgg cggcctcggc ggcctgctca 1470
tcggcctgct gctgtacacg tcccgcgcgg gcggcatcct gcagtcgaag ccgctgtact 1530
ggacgctcaa catcctggtg aacttcgtcc gcccgatccc cttcatcgtg ctcatcgcca 1590
tgctggcgcc ggtgacggtc caggtcgtgg gcaccaccat cggccggtcc gcggcgatct 1650
<210> 14
<211> 349
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 14
Val Thr Ala Pro Pro Thr Arg Pro Arg Thr Gly Thr Arg Ile Glu Phe
1 5 10 15
Arg Asp Val Thr Lys Val Phe Thr Asn Gln Arg Lys Thr Glu Thr Arg
20 25 30
Ala Val Asp Gly Val Thr Leu Thr Val Glu Pro Gly Glu Ile Leu Gly
35 40 45
Val Ile Gly Tyr Ser Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile
50 55 60
Asn Gly Leu Asp Ser Thr Thr Ser Gly Gln Leu Leu Leu Asp Gly Thr
65 70 75 80
Asp Ile Val Gly Leu Pro Glu Ser Lys Leu Arg Ser Ile Arg Arg Gly
85 90 95
Ile Gly Met Ile Phe Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala
100 105 110
Ala Gly Asn Ile Ala Tyr Pro Leu Glu Leu Ala Gly Met Pro Arg Ala
115 120 125
Glu Arg Arg Arg Arg Val Ala Glu Leu Leu Asp Phe Val Gly Leu Gly
130 135 140
Asp Lys Gly Asp Asn Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln
145 150 155 160
Arg Val Gly Ile Ala Arg Ala Leu Ala Thr Asn Pro Arg Leu Leu Leu
165 170 175
Ala Asp Glu Ala Thr Ser Ala Leu Asp Pro Glu Thr Thr Gln Asp Val
180 185 190
Leu Asp Leu Leu Arg Glu Ile Asn Arg Glu Leu Gly Ile Thr Ile Val
195 200 205
Val Ile Thr His Glu Met Asp Val Val Arg Ser Ile Ala Asp Lys Val
210 215 220
Ala Val Met Glu Ala Gly Arg Val Val Glu His Gly Ser Val Tyr Glu
225 230 235 240
Val Phe Ser Arg Pro Gln Thr Ala Val Ala Arg Arg Phe Val Ser Thr
245 250 255
Ser Leu Arg Asn Thr Pro Asp Gln Val Glu Ala Glu Asp Leu Leu Ala
260 265 270
His Glu Gly Arg Leu Phe Thr Ile Thr Leu Thr Glu Asp Ser Gly Phe
275 280 285
Phe Ala Ala Ala Ala Ala Gly Arg Glu Glu Gly Met Leu Ile Asn Ile
290 295 300
Val His Gly Gly Val Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met
305 310 315 320
Thr Val Arg Val Thr Gly Pro Asp Ala Ala Val Glu Lys Phe Arg Asp
325 330 335
Thr Leu Ser Arg Thr Thr Asp Ile Gln Glu Ile Thr Arg
340 345
<210> 15
<211> 1854
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1554)
<223> ген metK
<400> 15
gacggacaga ccagcgcgct cgagtacgcg caggccaagc tcacccgcaa gggctgcgac 60
ctgctcatgg ccaacgaggt gggggagggc cgggtcttcg gccagacccg caacagcggc 120
tggctgctca ccgccgacgg cgctgtcacg gaggttccgg acggttccaa gcacgtggtg 180
gcggcggcga tcctcagcgc cgtcgacgac ctgcgcaatt gacgctttta gaccgcttgg 240
tctatattgg gcgggacgct cagtcatatc attaatttga cccccgaaaa ggacccgccc 300
gtg tca gag aac ccc gcc acc acc acg gtg gcc ttc gac aag ccc gcc 348
Val Ser Glu Asn Pro Ala Thr Thr Thr Val Ala Phe Asp Lys Pro Ala
1 5 10 15
acg atc cgc ctg ttc acc agc gag tcc gtc acc gag ggc cac ccg gac 396
Thr Ile Arg Leu Phe Thr Ser Glu Ser Val Thr Glu Gly His Pro Asp
20 25 30
aag atc tgc gac gcc atc tcg gac acc atc ctc gac gcc atg ctc gag 444
Lys Ile Cys Asp Ala Ile Ser Asp Thr Ile Leu Asp Ala Met Leu Glu
35 40 45
cag gac ccg acc gcg cgc gtg gcc gtc gag acg ctc gtg acc acc ggc 492
Gln Asp Pro Thr Ala Arg Val Ala Val Glu Thr Leu Val Thr Thr Gly
50 55 60
cag gtc cac gtc gtc ggt gag gtc cgc acc acc ggc tac gtc gag atc 540
Gln Val His Val Val Gly Glu Val Arg Thr Thr Gly Tyr Val Glu Ile
65 70 75 80
ccg cag ctc gtg cgc aag acc ctc gtg gac atc ggc ttc atc tcc tcc 588
Pro Gln Leu Val Arg Lys Thr Leu Val Asp Ile Gly Phe Ile Ser Ser
85 90 95
gag gtc ggc ttc gac ggc acc acc tgc ggc gtc aac atc gcc atc ggc 636
Glu Val Gly Phe Asp Gly Thr Thr Cys Gly Val Asn Ile Ala Ile Gly
100 105 110
gag cag tcc cag gag atc gcc gac ggc gtc gac tcc tcc cac gag gag 684
Glu Gln Ser Gln Glu Ile Ala Asp Gly Val Asp Ser Ser His Glu Glu
115 120 125
cgt tcc ggc gtc gag atc gac gag gac gac cgc gcc ggc gcc ggc gac 732
Arg Ser Gly Val Glu Ile Asp Glu Asp Asp Arg Ala Gly Ala Gly Asp
130 135 140
cag ggc ctc atg ttc ggc tac gcc agc aac gag acc ccc gag tac atg 780
Gln Gly Leu Met Phe Gly Tyr Ala Ser Asn Glu Thr Pro Glu Tyr Met
145 150 155 160
ccg ctg ccc atc gcc ctg gcc cac cgc ctg gcc cgc cgc ctc acc gag 828
Pro Leu Pro Ile Ala Leu Ala His Arg Leu Ala Arg Arg Leu Thr Glu
165 170 175
gtg cgc aac cag ggc atc gtc ccg cac ctg cgt ccc gac ggc aag acc 876
Val Arg Asn Gln Gly Ile Val Pro His Leu Arg Pro Asp Gly Lys Thr
180 185 190
cag gtc acc ttc gcc tac gac gag cag gac cgc ccg gtc cgc ctc gac 924
Gln Val Thr Phe Ala Tyr Asp Glu Gln Asp Arg Pro Val Arg Leu Asp
195 200 205
acc gtg gtc atc tcc acg cag cac gac ccg gag gtc acc cag gac tgg 972
Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Val Thr Gln Asp Trp
210 215 220
ctg cac gag cag ctg cgc gag cac gtc ctc gac tgg gtc atc gcc gac 1020
Leu His Glu Gln Leu Arg Glu His Val Leu Asp Trp Val Ile Ala Asp
225 230 235 240
gcc ggc ctg atc ggc atg gtc gac gag gag ctc acc ctc ctg gtc aac 1068
Ala Gly Leu Ile Gly Met Val Asp Glu Glu Leu Thr Leu Leu Val Asn
245 250 255
ccg tcc ggc tcc ttc atc ctc ggc ggc ccg atg ggc gac gct ggc ctg 1116
Pro Ser Gly Ser Phe Ile Leu Gly Gly Pro Met Gly Asp Ala Gly Leu
260 265 270
acg ggc cgc aag atc atc gtc gac acc tac ggc ggc atg gcc cgc cac 1164
Thr Gly Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Met Ala Arg His
275 280 285
ggc ggc ggc gcc ttc tcc ggc aag gac ccg tcc aag gtg gac cgt tcc 1212
Gly Gly Gly Ala Phe Ser Gly Lys Asp Pro Ser Lys Val Asp Arg Ser
290 295 300
gcc gcg tac gcc atg cgc tgg gtg gcc aag aac atc gtc gcc gcc ggc 1260
Ala Ala Tyr Ala Met Arg Trp Val Ala Lys Asn Ile Val Ala Ala Gly
305 310 315 320
ctg gcc gag cgc gtc gag gtc cag gtg gcc tac gcc atc ggc cgc gcg 1308
Leu Ala Glu Arg Val Glu Val Gln Val Ala Tyr Ala Ile Gly Arg Ala
325 330 335
aag ccg gtc ggc ctc tac gtg gag tcc ttc ggc acc gcc gcc gcg ggc 1356
Lys Pro Val Gly Leu Tyr Val Glu Ser Phe Gly Thr Ala Ala Ala Gly
340 345 350
ctc agt gac gcg gac atc cag ggc gcc gtc gag aag gtc ttc gac ctg 1404
Leu Ser Asp Ala Asp Ile Gln Gly Ala Val Glu Lys Val Phe Asp Leu
355 360 365
cgg ccc gcc gcc atc atc cgc gag ctc gac ctg ctg cgc ccg atc tac 1452
Arg Pro Ala Ala Ile Ile Arg Glu Leu Asp Leu Leu Arg Pro Ile Tyr
370 375 380
gcg cag acc tcc gcc tac ggc cac ttc ggc cgc ctc gac ctg gat ctg 1500
Ala Gln Thr Ser Ala Tyr Gly His Phe Gly Arg Leu Asp Leu Asp Leu
385 390 395 400
ccc tgg gag cgg ctc aac cgg gtg gac gag ctc aag gcg gct ctg ggc 1548
Pro Trp Glu Arg Leu Asn Arg Val Asp Glu Leu Lys Ala Ala Leu Gly
405 410 415
cgc tag agtattcggc atggcctccc cccgcgtccc cgcagaacac ctgccggtgg 1604
Arg
cgcgggttct gcctctcctg ggcctggccc acctcgaccg gcccttcgac taccaggtgc 1664
cgtcgacgct ggatgacgac gcccagcccg gcgtccgcgt ccgcatccgc ttcgccgggc 1724
ggcttgtcga cgccgtcctc ctcgaacgcc gcgccgacac cgagcacgag ggcgggctga 1784
gctggatcga gcgggtgatc tccccgcacg tcgtgtaccc gccgcgcacc gcccggctca 1844
tcgaggcgct 1854
<210> 16
<211> 417
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 16
Val Ser Glu Asn Pro Ala Thr Thr Thr Val Ala Phe Asp Lys Pro Ala
1 5 10 15
Thr Ile Arg Leu Phe Thr Ser Glu Ser Val Thr Glu Gly His Pro Asp
20 25 30
Lys Ile Cys Asp Ala Ile Ser Asp Thr Ile Leu Asp Ala Met Leu Glu
35 40 45
Gln Asp Pro Thr Ala Arg Val Ala Val Glu Thr Leu Val Thr Thr Gly
50 55 60
Gln Val His Val Val Gly Glu Val Arg Thr Thr Gly Tyr Val Glu Ile
65 70 75 80
Pro Gln Leu Val Arg Lys Thr Leu Val Asp Ile Gly Phe Ile Ser Ser
85 90 95
Glu Val Gly Phe Asp Gly Thr Thr Cys Gly Val Asn Ile Ala Ile Gly
100 105 110
Glu Gln Ser Gln Glu Ile Ala Asp Gly Val Asp Ser Ser His Glu Glu
115 120 125
Arg Ser Gly Val Glu Ile Asp Glu Asp Asp Arg Ala Gly Ala Gly Asp
130 135 140
Gln Gly Leu Met Phe Gly Tyr Ala Ser Asn Glu Thr Pro Glu Tyr Met
145 150 155 160
Pro Leu Pro Ile Ala Leu Ala His Arg Leu Ala Arg Arg Leu Thr Glu
165 170 175
Val Arg Asn Gln Gly Ile Val Pro His Leu Arg Pro Asp Gly Lys Thr
180 185 190
Gln Val Thr Phe Ala Tyr Asp Glu Gln Asp Arg Pro Val Arg Leu Asp
195 200 205
Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Val Thr Gln Asp Trp
210 215 220
Leu His Glu Gln Leu Arg Glu His Val Leu Asp Trp Val Ile Ala Asp
225 230 235 240
Ala Gly Leu Ile Gly Met Val Asp Glu Glu Leu Thr Leu Leu Val Asn
245 250 255
Pro Ser Gly Ser Phe Ile Leu Gly Gly Pro Met Gly Asp Ala Gly Leu
260 265 270
Thr Gly Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Met Ala Arg His
275 280 285
Gly Gly Gly Ala Phe Ser Gly Lys Asp Pro Ser Lys Val Asp Arg Ser
290 295 300
Ala Ala Tyr Ala Met Arg Trp Val Ala Lys Asn Ile Val Ala Ala Gly
305 310 315 320
Leu Ala Glu Arg Val Glu Val Gln Val Ala Tyr Ala Ile Gly Arg Ala
325 330 335
Lys Pro Val Gly Leu Tyr Val Glu Ser Phe Gly Thr Ala Ala Ala Gly
340 345 350
Leu Ser Asp Ala Asp Ile Gln Gly Ala Val Glu Lys Val Phe Asp Leu
355 360 365
Arg Pro Ala Ala Ile Ile Arg Glu Leu Asp Leu Leu Arg Pro Ile Tyr
370 375 380
Ala Gln Thr Ser Ala Tyr Gly His Phe Gly Arg Leu Asp Leu Asp Leu
385 390 395 400
Pro Trp Glu Arg Leu Asn Arg Val Asp Glu Leu Lys Ala Ala Leu Gly
405 410 415
Arg
<210> 17
<211> 1278
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(978)
<223> ген metI
<400> 17
ggcgcggcgc ttcgtgtcca cctcgctgcg caacaccccc gaccaggtcg aggcggagga 60
cctgctggcc cacgagggcc gcctgttcac catcaccctc acggaggact ccgggttctt 120
cgccgcggcc gccgccggcc gtgaggaggg gatgctcatc aacatcgtcc acggcggcgt 180
caccacgctg cagcggcagt ccttcggcaa gatgaccgtc cgggtgacgg ggccggacgc 240
ggccgtcgag aagttccgtg acacgctgtc ccgcaccacc gacatccagg agatcacgcg 300
atg aac gag acc ctc ctc gcc gca gac tgg aac cgt ctc ggc ccg acc 348
Met Asn Glu Thr Leu Leu Ala Ala Asp Trp Asn Arg Leu Gly Pro Thr
1 5 10 15
ttc atc gac gcc atc ggc gac acc ctc gtc atg gtg tcc ctc acg atg 396
Phe Ile Asp Ala Ile Gly Asp Thr Leu Val Met Val Ser Leu Thr Met
20 25 30
gtg ctc ggc ggc ctc ggc ggc ctg ctc atc ggc ctg ctg ctg tac acg 444
Val Leu Gly Gly Leu Gly Gly Leu Leu Ile Gly Leu Leu Leu Tyr Thr
35 40 45
tcc cgc gcg ggc ggc atc ctg cag tcg aag ccg ctg tac tgg acg ctc 492
Ser Arg Ala Gly Gly Ile Leu Gln Ser Lys Pro Leu Tyr Trp Thr Leu
50 55 60
aac atc ctg gtg aac ttc gtc cgc ccg atc ccc ttc atc gtg ctc atc 540
Asn Ile Leu Val Asn Phe Val Arg Pro Ile Pro Phe Ile Val Leu Ile
65 70 75 80
gcc atg ctg gcg ccg gtg acg gtc cag gtc gtg ggc acc acc atc ggc 588
Ala Met Leu Ala Pro Val Thr Val Gln Val Val Gly Thr Thr Ile Gly
85 90 95
cgg tcc gcg gcg atc ttc gtc atg tgc atc gcc gcc gcc ttc gcc gtg 636
Arg Ser Ala Ala Ile Phe Val Met Cys Ile Ala Ala Ala Phe Ala Val
100 105 110
gcc cgc atc gtc gag cag aac ctc gtg gcc atc gac ccc ggc gtc atc 684
Ala Arg Ile Val Glu Gln Asn Leu Val Ala Ile Asp Pro Gly Val Ile
115 120 125
gag gcc gcc cgc gcc atg ggg gcg ggg ccg tgg cgg atc atc cgc acg 732
Glu Ala Ala Arg Ala Met Gly Ala Gly Pro Trp Arg Ile Ile Arg Thr
130 135 140
gtg atc atc ccg gag gcc ctc ggc ccg ctg gtc ctc ggc tac acg ttc 780
Val Ile Ile Pro Glu Ala Leu Gly Pro Leu Val Leu Gly Tyr Thr Phe
145 150 155 160
atc ttc atc gcg gtg gtc gac atg tcc gcg atg gcc ggc tac atc ggc 828
Ile Phe Ile Ala Val Val Asp Met Ser Ala Met Ala Gly Tyr Ile Gly
165 170 175
ggt ggt ggc ctg ggt gac ttc gcc atc gtc tac ggc tac cgc gcc tac 876
Gly Gly Gly Leu Gly Asp Phe Ala Ile Val Tyr Gly Tyr Arg Ala Tyr
180 185 190
gac ctg gag gtc acg tac gcg gcg gtc atc gtc atc gtc atc atc gtg 924
Asp Leu Glu Val Thr Tyr Ala Ala Val Ile Val Ile Val Ile Ile Val
195 200 205
cag atc gcg cag ctg ctg ggc aac tgg ctc tcg cga aag ctc atg cgg 972
Gln Ile Ala Gln Leu Leu Gly Asn Trp Leu Ser Arg Lys Leu Met Arg
210 215 220
cgt tag cgtcgcccgc cgttcacacc cccgaccccg tgtcgggggt tttgcgtacc 1028
Arg
225
cggagccgaa acgggggctc ccgatcgaga acacgtcggg gcgacgacct gccgtgcgga 1088
agtacccttc tgcccgctcg accgcactgt ttccccagga cacaacctgg cctgcagaca 1148
cagacgggta cctcagaacg cagctagcct ggatgtcatg aagatcgccg ccttcgacat 1208
cgacgacacc ctgctgttcc cccacggcat cgccgaggag gacatccgcg ccatccgcgc 1268
ctggcaggag 1278
<210> 18
<211> 225
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 18
Met Asn Glu Thr Leu Leu Ala Ala Asp Trp Asn Arg Leu Gly Pro Thr
1 5 10 15
Phe Ile Asp Ala Ile Gly Asp Thr Leu Val Met Val Ser Leu Thr Met
20 25 30
Val Leu Gly Gly Leu Gly Gly Leu Leu Ile Gly Leu Leu Leu Tyr Thr
35 40 45
Ser Arg Ala Gly Gly Ile Leu Gln Ser Lys Pro Leu Tyr Trp Thr Leu
50 55 60
Asn Ile Leu Val Asn Phe Val Arg Pro Ile Pro Phe Ile Val Leu Ile
65 70 75 80
Ala Met Leu Ala Pro Val Thr Val Gln Val Val Gly Thr Thr Ile Gly
85 90 95
Arg Ser Ala Ala Ile Phe Val Met Cys Ile Ala Ala Ala Phe Ala Val
100 105 110
Ala Arg Ile Val Glu Gln Asn Leu Val Ala Ile Asp Pro Gly Val Ile
115 120 125
Glu Ala Ala Arg Ala Met Gly Ala Gly Pro Trp Arg Ile Ile Arg Thr
130 135 140
Val Ile Ile Pro Glu Ala Leu Gly Pro Leu Val Leu Gly Tyr Thr Phe
145 150 155 160
Ile Phe Ile Ala Val Val Asp Met Ser Ala Met Ala Gly Tyr Ile Gly
165 170 175
Gly Gly Gly Leu Gly Asp Phe Ala Ile Val Tyr Gly Tyr Arg Ala Tyr
180 185 190
Asp Leu Glu Val Thr Tyr Ala Ala Val Ile Val Ile Val Ile Ile Val
195 200 205
Gln Ile Ala Gln Leu Leu Gly Asn Trp Leu Ser Arg Lys Leu Met Arg
210 215 220
Arg
225
<210> 19
<211> 1509
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1209)
<223> ген dapA
<400> 19
ggcaggccgc ccgcgcggtg ctgccgaacg ccacggagtc acgcatcgtc gtcaccggca 60
actaccgggc gtggcgccac ttcatcggcg cccgggccac cgagcacgcg gacgtcgaga 120
tccgggcact ggcggtggcg tgcctccgcc tcctcgcgca gcagtccccg gtgctcttcg 180
ccgatttcca gatctccact ctctccgacg gcacagagat ggccgtcagt ccctacgcca 240
ccgacttctg accagcgagg gattccccag cggaaaggta aacaggtaac cttgaacacc 300
atg agt aca ggt atg act tcg aag acc gga gtc gag gac ttc ggt acc 348
Met Ser Thr Gly Met Thr Ser Lys Thr Gly Val Glu Asp Phe Gly Thr
1 5 10 15
gtc tgc gtc gcc atg gtc acg ccc ttc gac cgg gac ggc gcc ctg gac 396
Val Cys Val Ala Met Val Thr Pro Phe Asp Arg Asp Gly Ala Leu Asp
20 25 30
ctc gag gcc ggt cgc cgc atc gcg gcc cac ctc gtc gac aac ggt ctg 444
Leu Glu Ala Gly Arg Arg Ile Ala Ala His Leu Val Asp Asn Gly Leu
35 40 45
gac gcc ctc gtg ctc gca ggc acg acc ggt gag tcc ccg acc acc acg 492
Asp Ala Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr
50 55 60
ctc gac gag aag atc agc ctg ctc aag gcg gtg aag gag gag gtc ggc 540
Leu Asp Glu Lys Ile Ser Leu Leu Lys Ala Val Lys Glu Glu Val Gly
65 70 75 80
gac cgc tgc aag ctc atc gcg ggt gcc ggc acc aac gac acc gcc tcc 588
Asp Arg Cys Lys Leu Ile Ala Gly Ala Gly Thr Asn Asp Thr Ala Ser
85 90 95
tcc atc gag ctc gcc cgc gcc tcc gcc gag gcc ggc gcc gac gcc ctg 636
Ser Ile Glu Leu Ala Arg Ala Ser Ala Glu Ala Gly Ala Asp Ala Leu
100 105 110
ctc gtg gtc acc ccc tac tac tcg aag ccg agc cag gag ggt gtc tac 684
Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Val Tyr
115 120 125
cag cac ttc aag gcc gtc gcc ggg gcc acc gac ctg ccc atc atg ctc 732
Gln His Phe Lys Ala Val Ala Gly Ala Thr Asp Leu Pro Ile Met Leu
130 135 140
tac gac atc ccg ccg cgc tcc tcg atc ccg atc gag ggg gac acc atc 780
Tyr Asp Ile Pro Pro Arg Ser Ser Ile Pro Ile Glu Gly Asp Thr Ile
145 150 155 160
cgc cga ctc gcg gag ctg ccc acc att cgt gcg gtg aag gat gca aag 828
Arg Arg Leu Ala Glu Leu Pro Thr Ile Arg Ala Val Lys Asp Ala Lys
165 170 175
ggt aac atg gct gag tct gca ccg ctc atg cat gag acc ggt ctc gcc 876
Gly Asn Met Ala Glu Ser Ala Pro Leu Met His Glu Thr Gly Leu Ala
180 185 190
tgg tac tcg ggc gat gat ccg ctc aac gtc ccg tgg ctg tcg ctc ggc 924
Trp Tyr Ser Gly Asp Asp Pro Leu Asn Val Pro Trp Leu Ser Leu Gly
195 200 205
gcc tcg gga ttc gtt tcc gtg gtc ggc cat gcc gca ccc cag gca ctg 972
Ala Ser Gly Phe Val Ser Val Val Gly His Ala Ala Pro Gln Ala Leu
210 215 220
cgt gag ctg tac aca agt ttc gag gaa ggc gac ctc gcc cgt gcg cgg 1020
Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Ala Arg Ala Arg
225 230 235 240
gaa atc aac gcc act gtt ctt tca ccg ctg atc gcc gct cag ggt cgc 1068
Glu Ile Asn Ala Thr Val Leu Ser Pro Leu Ile Ala Ala Gln Gly Arg
245 250 255
ctg ggt ggt gtc agc atg gcc aag gct gcc ctg cgt ctg cag ggc att 1116
Leu Gly Gly Val Ser Met Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile
260 265 270
gag gtg ggg gac ccg cgt ctg cct gtc gtc gct ccc gac gag cag gag 1164
Glu Val Gly Asp Pro Arg Leu Pro Val Val Ala Pro Asp Glu Gln Glu
275 280 285
atc gag gcc ctc cgc cgt gac atg gaa aag gct gga gtc ctt taa 1209
Ile Glu Ala Leu Arg Arg Asp Met Glu Lys Ala Gly Val Leu
290 295 300
atatgactga atcccgtagt cgtgcccgga aggtcacccg caaggcgggc ccgccggaga 1269
ccgagagcac tggtgcatcc ccggttttcc aggcaccgac cccggaaacg aacggaaacg 1329
gcgcatccgc cgagaagaag accaccgaaa ggaagtccgc ggacaccgac aacgccccgc 1389
ggggcggcgg caaccgctcc cggaacggtg gcggccgcgc ccgcggcggc cgtggtggcc 1449
gcggcggtca gggtggccag ggccagggcg gtcagggtgg tcagcagggg ggacggggac 1509
<210> 20
<211> 302
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 20
Met Ser Thr Gly Met Thr Ser Lys Thr Gly Val Glu Asp Phe Gly Thr
1 5 10 15
Val Cys Val Ala Met Val Thr Pro Phe Asp Arg Asp Gly Ala Leu Asp
20 25 30
Leu Glu Ala Gly Arg Arg Ile Ala Ala His Leu Val Asp Asn Gly Leu
35 40 45
Asp Ala Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr
50 55 60
Leu Asp Glu Lys Ile Ser Leu Leu Lys Ala Val Lys Glu Glu Val Gly
65 70 75 80
Asp Arg Cys Lys Leu Ile Ala Gly Ala Gly Thr Asn Asp Thr Ala Ser
85 90 95
Ser Ile Glu Leu Ala Arg Ala Ser Ala Glu Ala Gly Ala Asp Ala Leu
100 105 110
Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Val Tyr
115 120 125
Gln His Phe Lys Ala Val Ala Gly Ala Thr Asp Leu Pro Ile Met Leu
130 135 140
Tyr Asp Ile Pro Pro Arg Ser Ser Ile Pro Ile Glu Gly Asp Thr Ile
145 150 155 160
Arg Arg Leu Ala Glu Leu Pro Thr Ile Arg Ala Val Lys Asp Ala Lys
165 170 175
Gly Asn Met Ala Glu Ser Ala Pro Leu Met His Glu Thr Gly Leu Ala
180 185 190
Trp Tyr Ser Gly Asp Asp Pro Leu Asn Val Pro Trp Leu Ser Leu Gly
195 200 205
Ala Ser Gly Phe Val Ser Val Val Gly His Ala Ala Pro Gln Ala Leu
210 215 220
Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Ala Arg Ala Arg
225 230 235 240
Glu Ile Asn Ala Thr Val Leu Ser Pro Leu Ile Ala Ala Gln Gly Arg
245 250 255
Leu Gly Gly Val Ser Met Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile
260 265 270
Glu Val Gly Asp Pro Arg Leu Pro Val Val Ala Pro Asp Glu Gln Glu
275 280 285
Ile Glu Ala Leu Arg Arg Asp Met Glu Lys Ala Gly Val Leu
290 295 300
<210> 21
<211> 1515
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1215)
<223> CBS
<400> 21
gggcctccgc gacgggagcg ctggaaccac cgcgccaggg gccctgtgcc tcggccacct 60
gagtggcgga cagggtgagg gcgacggcca gggcggccgc gctgatctga cggtgtcggc 120
gggtgaaagc catgttcatc atctcctatg agctctattg gcgtaagggt ggatgatagg 180
gcagaaaatg caggttagat accctgccgg gtatgggata tctcacgggc tgcgacggga 240
acttcgcggt ccggaaaaag ttcccgcctt cccttgactt tgcggcgtaa gatgtcattt 300
atg agt ctc gag ccc ggg aac acc ccg ctc gta cgt ctg gac cgg ctc 348
Met Ser Leu Glu Pro Gly Asn Thr Pro Leu Val Arg Leu Asp Arg Leu
1 5 10 15
ccc ggc cgg ccc ggt gtc acc ctg tgg gcg aag ctg gag ggc cac aac 396
Pro Gly Arg Pro Gly Val Thr Leu Trp Ala Lys Leu Glu Gly His Asn
20 25 30
ccc agc ggc agt gcc aag gac cgg acc gcg cac gcc atc ctc gcg gcg 444
Pro Ser Gly Ser Ala Lys Asp Arg Thr Ala His Ala Ile Leu Ala Ala
35 40 45
gcg gat ctg cgg ccc ggc gcg gtc atc gtc gag tcg agc tcg ggc aac 492
Ala Asp Leu Arg Pro Gly Ala Val Ile Val Glu Ser Ser Ser Gly Asn
50 55 60
ctc ggc gtc gcc ctg gcc cgg gag gcg gtc ctc ggc ggc tgg acc ttc 540
Leu Gly Val Ala Leu Ala Arg Glu Ala Val Leu Gly Gly Trp Thr Phe
65 70 75 80
cac tgt gtc gtc gat ccg aag gcg aat cgc tcg acc atc gcc acc atg 588
His Cys Val Val Asp Pro Lys Ala Asn Arg Ser Thr Ile Ala Thr Met
85 90 95
cgc gcg ttc ggg gcg gtc gtc cac gag gtc acc gca ccc gat ccg gcc 636
Arg Ala Phe Gly Ala Val Val His Glu Val Thr Ala Pro Asp Pro Ala
100 105 110
acc ggt gac tgg ctc gcc gcc cgg cgc gcg cgg gtc gcg gaa ctg ctc 684
Thr Gly Asp Trp Leu Ala Ala Arg Arg Ala Arg Val Ala Glu Leu Leu
115 120 125
acc gag atc ccc ggg gcc gtc tgc ctc gac cag tac tcc aac cgg gcg 732
Thr Glu Ile Pro Gly Ala Val Cys Leu Asp Gln Tyr Ser Asn Arg Ala
130 135 140
gcc ttc acg gcc cac gac gag ggc acg atg gcc gag atc gtc gcg cag 780
Ala Phe Thr Ala His Asp Glu Gly Thr Met Ala Glu Ile Val Ala Gln
145 150 155 160
ctc ggc cac acc ccc gcg cac ctg ttc gtg gcg gtg agc acc acc ggc 828
Leu Gly His Thr Pro Ala His Leu Phe Val Ala Val Ser Thr Thr Gly
165 170 175
acg atc ggc ggc tgc ctg cgc cgc atc gcc cgg gag ggg tgg gac acg 876
Thr Ile Gly Gly Cys Leu Arg Arg Ile Ala Arg Glu Gly Trp Asp Thr
180 185 190
gag gtc acc gcc gtc gac gcc gag ggt tcc gtg ctt ttc gac ggc gcc 924
Glu Val Thr Ala Val Asp Ala Glu Gly Ser Val Leu Phe Asp Gly Ala
195 200 205
cgt ggc cgc cgc cac ctt ccc ggc ttc ggc gcc ggc atc gtc ccg gac 972
Arg Gly Arg Arg His Leu Pro Gly Phe Gly Ala Gly Ile Val Pro Asp
210 215 220
ctg gcc gcc gag gtg acc ccg gac cgc atc gtc cgg gtc ggc gac cgt 1020
Leu Ala Ala Glu Val Thr Pro Asp Arg Ile Val Arg Val Gly Asp Arg
225 230 235 240
gac tcc gtc gcc gcc gcg cgg cag ctg gcc cgc acg gaa ggg gtg ttg 1068
Asp Ser Val Ala Ala Ala Arg Gln Leu Ala Arg Thr Glu Gly Val Leu
245 250 255
tgc ggt gcc tcg ggt ggg gcg gtg gtg gcg gcc gtc ggg aag ctc ctg 1116
Cys Gly Ala Ser Gly Gly Ala Val Val Ala Ala Val Gly Lys Leu Leu
260 265 270
ccg gag ctg cac ggg gac gtc gtc ctc gtg ctc cac gac ggt ggc acc 1164
Pro Glu Leu His Gly Asp Val Val Leu Val Leu His Asp Gly Gly Thr
275 280 285
cac tac ctg gac acc gtg tac aac gac gac tgg gtg gag gag aac ctg 1212
His Tyr Leu Asp Thr Val Tyr Asn Asp Asp Trp Val Glu Glu Asn Leu
290 295 300
tga caaccgtggt gatcatcggc ggcggcccgc gcggactgtg ggcggcggag 1265
gaactgctgg gcctggcacg cgagaggggc gcggccctcg acgtccacgt catcgacgac 1325
ggcggaccgc aggcctacga cccggggcag ccggaggagt ggctcatcaa cgtgcgggcc 1385
tccgtgatcc gcacggggct gggctccttc gacgagtggc gcggggccgc cgaccccttc 1445
ccgccgcgac gcgaggtggc ccgtttcctc gccgcctcct gggaggagct cgcacagcac 1505
gtgtcgccgc 1515
<210> 22
<211> 304
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 22
Met Ser Leu Glu Pro Gly Asn Thr Pro Leu Val Arg Leu Asp Arg Leu
1 5 10 15
Pro Gly Arg Pro Gly Val Thr Leu Trp Ala Lys Leu Glu Gly His Asn
20 25 30
Pro Ser Gly Ser Ala Lys Asp Arg Thr Ala His Ala Ile Leu Ala Ala
35 40 45
Ala Asp Leu Arg Pro Gly Ala Val Ile Val Glu Ser Ser Ser Gly Asn
50 55 60
Leu Gly Val Ala Leu Ala Arg Glu Ala Val Leu Gly Gly Trp Thr Phe
65 70 75 80
His Cys Val Val Asp Pro Lys Ala Asn Arg Ser Thr Ile Ala Thr Met
85 90 95
Arg Ala Phe Gly Ala Val Val His Glu Val Thr Ala Pro Asp Pro Ala
100 105 110
Thr Gly Asp Trp Leu Ala Ala Arg Arg Ala Arg Val Ala Glu Leu Leu
115 120 125
Thr Glu Ile Pro Gly Ala Val Cys Leu Asp Gln Tyr Ser Asn Arg Ala
130 135 140
Ala Phe Thr Ala His Asp Glu Gly Thr Met Ala Glu Ile Val Ala Gln
145 150 155 160
Leu Gly His Thr Pro Ala His Leu Phe Val Ala Val Ser Thr Thr Gly
165 170 175
Thr Ile Gly Gly Cys Leu Arg Arg Ile Ala Arg Glu Gly Trp Asp Thr
180 185 190
Glu Val Thr Ala Val Asp Ala Glu Gly Ser Val Leu Phe Asp Gly Ala
195 200 205
Arg Gly Arg Arg His Leu Pro Gly Phe Gly Ala Gly Ile Val Pro Asp
210 215 220
Leu Ala Ala Glu Val Thr Pro Asp Arg Ile Val Arg Val Gly Asp Arg
225 230 235 240
Asp Ser Val Ala Ala Ala Arg Gln Leu Ala Arg Thr Glu Gly Val Leu
245 250 255
Cys Gly Ala Ser Gly Gly Ala Val Val Ala Ala Val Gly Lys Leu Leu
260 265 270
Pro Glu Leu His Gly Asp Val Val Leu Val Leu His Asp Gly Gly Thr
275 280 285
His Tyr Leu Asp Thr Val Tyr Asn Asp Asp Trp Val Glu Glu Asn Leu
290 295 300
<210> 23
<211> 1746
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1446)
<223> ген cg3031-гомолог
<400> 23
cccggagccg gagcgcctca acgtgctcaa caactcgacg gtgccgaacc tggccgcgca 60
ggtcgcggac cgcctgcggg aggacggttt cgaggtcggg gaggtcggca acctggcgga 120
ccacatcctc ccggagacca ccgtcttctt ccagcccggc aacgccgccg cggagcagcg 180
ggcgcgggag ctcgccgacc gcctcggcgg cgtcgcccgc gagtacgagg gcttcctccc 240
ggagaacacc gccggtcgca acgacctgac tctggtgctg gtgaaccagg tggcgctctg 300
atg ggc ccg ggg acc ggc atc gag ccg atc ccg ttc cgc cgc tcc ccg 348
Met Gly Pro Gly Thr Gly Ile Glu Pro Ile Pro Phe Arg Arg Ser Pro
1 5 10 15
cgg ccg acg ctc ggc gtc gag tgg gag atc gcg ctc atc gac ccc gag 396
Arg Pro Thr Leu Gly Val Glu Trp Glu Ile Ala Leu Ile Asp Pro Glu
20 25 30
acc cgc gac ctg gcg ccc aag gcc gtc gag gtc atc gac ctc gtc cgg 444
Thr Arg Asp Leu Ala Pro Lys Ala Val Glu Val Ile Asp Leu Val Arg
35 40 45
tcc cgc cac ccg gag gtg cac ctg gag aag gag ttc ctg cag aac acc 492
Ser Arg His Pro Glu Val His Leu Glu Lys Glu Phe Leu Gln Asn Thr
50 55 60
gtc gaa ctg gtc acc ggc atc cac gaa acc gtg ccg gag gcg gtc gcg 540
Val Glu Leu Val Thr Gly Ile His Glu Thr Val Pro Glu Ala Val Ala
65 70 75 80
gaa ctc gcc cgc gac ctg gcc gcc gtg aag gag gcc gcc gcc gac ctc 588
Glu Leu Ala Arg Asp Leu Ala Ala Val Lys Glu Ala Ala Ala Asp Leu
85 90 95
gga ctg tgc ctg tgg tcc tcc ggc tcg cac ccc ttc tcg gac tgg cgg 636
Gly Leu Cys Leu Trp Ser Ser Gly Ser His Pro Phe Ser Asp Trp Arg
100 105 110
aag aac ccg gtc agc gcg aag atg gcc tac gcc gag atc atc gag cgc 684
Lys Asn Pro Val Ser Ala Lys Met Ala Tyr Ala Glu Ile Ile Glu Arg
115 120 125
acc cgc tac tgg ggt tcg cag atg ctc atc tgg ggc atc cac gtg cac 732
Thr Arg Tyr Trp Gly Ser Gln Met Leu Ile Trp Gly Ile His Val His
130 135 140
gtc ggg atc agc cac gag gac cgg gtg tgg ccg atc atc aac gcc ctg 780
Val Gly Ile Ser His Glu Asp Arg Val Trp Pro Ile Ile Asn Ala Leu
145 150 155 160
atg acc cgc tac ccg cac ctg ctg gcg ctg acg gcg tcg tcg ccg ggc 828
Met Thr Arg Tyr Pro His Leu Leu Ala Leu Thr Ala Ser Ser Pro Gly
165 170 175
tgg gac ggc atc gac acg ggc tac gcc tcc aac cgc acg atg ctc tac 876
Trp Asp Gly Ile Asp Thr Gly Tyr Ala Ser Asn Arg Thr Met Leu Tyr
180 185 190
cag cag ctg ccc acc gcc ggc ctg ccc ttc cag ttc cgg aac tgg gcc 924
Gln Gln Leu Pro Thr Ala Gly Leu Pro Phe Gln Phe Arg Asn Trp Ala
195 200 205
gag tgg gag tcc tac atg cac gac cag ggc atc tcg ggc gtg atc aac 972
Glu Trp Glu Ser Tyr Met His Asp Gln Gly Ile Ser Gly Val Ile Asn
210 215 220
cac acc ggg tcg atg cac ttc gac atc cgt ccc gcc ccg aag tgg ggc 1020
His Thr Gly Ser Met His Phe Asp Ile Arg Pro Ala Pro Lys Trp Gly
225 230 235 240
acg gtc gag gtg cgg gtc tcc gac gcc acc tcc aac ctg cgg gaa ctc 1068
Thr Val Glu Val Arg Val Ser Asp Ala Thr Ser Asn Leu Arg Glu Leu
245 250 255
gcc gcc gtc gtg gcg ctc acg cac tgc ctg gtc gtc cac tac gac cgc 1116
Ala Ala Val Val Ala Leu Thr His Cys Leu Val Val His Tyr Asp Arg
260 265 270
atg atc gac cgc ggt gag gaa ctg ccc gtc ctg cag ccc tgg cac gtc 1164
Met Ile Asp Arg Gly Glu Glu Leu Pro Val Leu Gln Pro Trp His Val
275 280 285
gcc gag aac aag tgg cgg gcc gcc cgc tac ggg ctg gag gca ctc gtg 1212
Ala Glu Asn Lys Trp Arg Ala Ala Arg Tyr Gly Leu Glu Ala Leu Val
290 295 300
atc acc agc cgt gac acc gac gag cgc tgg gtc acc cag gag ctc gcg 1260
Ile Thr Ser Arg Asp Thr Asp Glu Arg Trp Val Thr Gln Glu Leu Ala
305 310 315 320
gag ctt gtc gac gaa ctc gcg ccc ctc gcc acc gaa ctc ggc tgc ctc 1308
Glu Leu Val Asp Glu Leu Ala Pro Leu Ala Thr Glu Leu Gly Cys Leu
325 330 335
gcc gaa ctc cag ctg gtc tcc gag atc ctc gaa cgt ggc gcc ggc tac 1356
Ala Glu Leu Gln Leu Val Ser Glu Ile Leu Glu Arg Gly Ala Gly Tyr
340 345 350
cag cgt cag cgt cgc atc cac gac gtc acc ggc agc tgg atc ccc gtc 1404
Gln Arg Gln Arg Arg Ile His Asp Val Thr Gly Ser Trp Ile Pro Val
355 360 365
atc gac gcc gcc tgc cgg gag atg gag gag atg gtc ccg tga 1446
Ile Asp Ala Ala Cys Arg Glu Met Glu Glu Met Val Pro
370 375 380
gcgacgtcgt cctcgtccac tccaccgggc acctgccgga cgtgtggacg gaggtggtgc 1506
gcgccttccc ggccgactgg aagccgtggc tgcccaatct gggcgtgcag tccctcgagc 1566
agtacctgga ccggcaggag ctgcgccggg tggtgctcgt cggtcacggc accggcgccc 1626
tggccgtggc acgcctggcc cgggagcagc cgcagcgggt cacccacgcg gtgctgtccg 1686
tgccggaggc gccccgcctc gtgccacgtt tcctgcggcg ccggcaggag gaggccctgg 1746
<210> 24
<211> 381
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 24
Met Gly Pro Gly Thr Gly Ile Glu Pro Ile Pro Phe Arg Arg Ser Pro
1 5 10 15
Arg Pro Thr Leu Gly Val Glu Trp Glu Ile Ala Leu Ile Asp Pro Glu
20 25 30
Thr Arg Asp Leu Ala Pro Lys Ala Val Glu Val Ile Asp Leu Val Arg
35 40 45
Ser Arg His Pro Glu Val His Leu Glu Lys Glu Phe Leu Gln Asn Thr
50 55 60
Val Glu Leu Val Thr Gly Ile His Glu Thr Val Pro Glu Ala Val Ala
65 70 75 80
Glu Leu Ala Arg Asp Leu Ala Ala Val Lys Glu Ala Ala Ala Asp Leu
85 90 95
Gly Leu Cys Leu Trp Ser Ser Gly Ser His Pro Phe Ser Asp Trp Arg
100 105 110
Lys Asn Pro Val Ser Ala Lys Met Ala Tyr Ala Glu Ile Ile Glu Arg
115 120 125
Thr Arg Tyr Trp Gly Ser Gln Met Leu Ile Trp Gly Ile His Val His
130 135 140
Val Gly Ile Ser His Glu Asp Arg Val Trp Pro Ile Ile Asn Ala Leu
145 150 155 160
Met Thr Arg Tyr Pro His Leu Leu Ala Leu Thr Ala Ser Ser Pro Gly
165 170 175
Trp Asp Gly Ile Asp Thr Gly Tyr Ala Ser Asn Arg Thr Met Leu Tyr
180 185 190
Gln Gln Leu Pro Thr Ala Gly Leu Pro Phe Gln Phe Arg Asn Trp Ala
195 200 205
Glu Trp Glu Ser Tyr Met His Asp Gln Gly Ile Ser Gly Val Ile Asn
210 215 220
His Thr Gly Ser Met His Phe Asp Ile Arg Pro Ala Pro Lys Trp Gly
225 230 235 240
Thr Val Glu Val Arg Val Ser Asp Ala Thr Ser Asn Leu Arg Glu Leu
245 250 255
Ala Ala Val Val Ala Leu Thr His Cys Leu Val Val His Tyr Asp Arg
260 265 270
Met Ile Asp Arg Gly Glu Glu Leu Pro Val Leu Gln Pro Trp His Val
275 280 285
Ala Glu Asn Lys Trp Arg Ala Ala Arg Tyr Gly Leu Glu Ala Leu Val
290 295 300
Ile Thr Ser Arg Asp Thr Asp Glu Arg Trp Val Thr Gln Glu Leu Ala
305 310 315 320
Glu Leu Val Asp Glu Leu Ala Pro Leu Ala Thr Glu Leu Gly Cys Leu
325 330 335
Ala Glu Leu Gln Leu Val Ser Glu Ile Leu Glu Arg Gly Ala Gly Tyr
340 345 350
Gln Arg Gln Arg Arg Ile His Asp Val Thr Gly Ser Trp Ile Pro Val
355 360 365
Ile Asp Ala Ala Cys Arg Glu Met Glu Glu Met Val Pro
370 375 380
<210> 25
<211> 1731
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1431)
<223> ген aecD
<400> 25
aggccgaacg cgccgtccag gccggcctgg acgagctgat gaaggaccgc accacgctca 60
tcatcgcgca ccgcctgtcg acgatcgcgg gtgtggacac catcgtcacc ctccgcgacg 120
gcatggtcga cgaaataggt tcaccggacc agctcgccgc ctcgggtggc atctacgccg 180
aactcctgca gctcacggcg tcctcctcgg ccgctgaccg ggcccgtctg aagaggtacg 240
ggctcgtcgt cgacggtcac ggcgacgagg aggacgagga agaggactag tctgtctggc 300
atg cag ttc ccc acc ctc gac cag ctc aag gac cgt cgc acc cgc aag 348
Met Gln Phe Pro Thr Leu Asp Gln Leu Lys Asp Arg Arg Thr Arg Lys
1 5 10 15
tgg acg gtc tac gcc gac gac gtc ctg ccc ctg tgg atc gcg gag agt 396
Trp Thr Val Tyr Ala Asp Asp Val Leu Pro Leu Trp Ile Ala Glu Ser
20 25 30
gac ttc ttc acc tgc ccg ccg gtg aag cag gcg atc gcg gac gcc atc 444
Asp Phe Phe Thr Cys Pro Pro Val Lys Gln Ala Ile Ala Asp Ala Ile
35 40 45
gac cgg gag tcc ttc ggg tac acc ccg gcg acc tcc gac ctg ccg cag 492
Asp Arg Glu Ser Phe Gly Tyr Thr Pro Ala Thr Ser Asp Leu Pro Gln
50 55 60
gcg gtc tcc gac ttc tac gcc gca cgt tac ggc tgg cgc ccg gac ccg 540
Ala Val Ser Asp Phe Tyr Ala Ala Arg Tyr Gly Trp Arg Pro Asp Pro
65 70 75 80
gcg ctc atc gtc gcg gtc ccc gac gtc gtc cgt ggc ctg gcc ctc gcc 588
Ala Leu Ile Val Ala Val Pro Asp Val Val Arg Gly Leu Ala Leu Ala
85 90 95
atc gag tac atg acg cgc ccg gac tcg ggt gtc atc gtg ccg gtg ccg 636
Ile Glu Tyr Met Thr Arg Pro Asp Ser Gly Val Ile Val Pro Val Pro
100 105 110
gcg tac ccg ccg ttc ctg gag ctg ccg gag gcg acc cgc cgt ccg atg 684
Ala Tyr Pro Pro Phe Leu Glu Leu Pro Glu Ala Thr Arg Arg Pro Met
115 120 125
cac cag atc gac gcc tac ggc ggg atc gac ctg ggg gac gtc gag aag 732
His Gln Ile Asp Ala Tyr Gly Gly Ile Asp Leu Gly Asp Val Glu Lys
130 135 140
cag ttc gcg gcc ggc gcc ggc tcg atc ctc ctg tgc gcc ccg aac aac 780
Gln Phe Ala Ala Gly Ala Gly Ser Ile Leu Leu Cys Ala Pro Asn Asn
145 150 155 160
ccc ctg ggg tac acg ttc gac gcg gag ttc ctg cgg gag ctg tgc gac 828
Pro Leu Gly Tyr Thr Phe Asp Ala Glu Phe Leu Arg Glu Leu Cys Asp
165 170 175
ctg gcg gac cgt tac gac gcc cgc gtg ctc gtc gac gag atc cac gcc 876
Leu Ala Asp Arg Tyr Asp Ala Arg Val Leu Val Asp Glu Ile His Ala
180 185 190
ccg ctg gtg ttc gac ggc acc cat gtg tgc gcc gcc gcc gtc agc gag 924
Pro Leu Val Phe Asp Gly Thr His Val Cys Ala Ala Ala Val Ser Glu
195 200 205
acc gcc gcg cgg gtg tgc gtc acc gtc acg gcg acg tcg aag gcg tgg 972
Thr Ala Ala Arg Val Cys Val Thr Val Thr Ala Thr Ser Lys Ala Trp
210 215 220
aac atc gcg ggc ctc aag tgc gcg cag atg atc ttc tcc aac ccg gcg 1020
Asn Ile Ala Gly Leu Lys Cys Ala Gln Met Ile Phe Ser Asn Pro Ala
225 230 235 240
gac atg gac acg tgg cgg tcg atg acc ggc gtg gcc aag gac ggc acc 1068
Asp Met Asp Thr Trp Arg Ser Met Thr Gly Val Ala Lys Asp Gly Thr
245 250 255
tcc acc ctg ggc atc tgg gcg gcg atc gcc tgc tac cgg gag ggc ggc 1116
Ser Thr Leu Gly Ile Trp Ala Ala Ile Ala Cys Tyr Arg Glu Gly Gly
260 265 270
gac tgc ctc gac gag cag gtc gcg tac ctg cgg gag aac cgc gac tgg 1164
Asp Cys Leu Asp Glu Gln Val Ala Tyr Leu Arg Glu Asn Arg Asp Trp
275 280 285
ctg gcc gag gag ctg ccg cgc cgc gtg ccg ggg ctg cag gtg atc agc 1212
Leu Ala Glu Glu Leu Pro Arg Arg Val Pro Gly Leu Gln Val Ile Ser
290 295 300
ccg gcg gcg acc tat ctc atg tgg atg gac ttc tcc ggc acc tcc atc 1260
Pro Ala Ala Thr Tyr Leu Met Trp Met Asp Phe Ser Gly Thr Ser Ile
305 310 315 320
ggg cac ctg gag cgc ccg gcg gtg tgg ctg cgg gag aac gcg aag gtc 1308
Gly His Leu Glu Arg Pro Ala Val Trp Leu Arg Glu Asn Ala Lys Val
325 330 335
gcc ctc aac gac ggc gtc gcc ttc ggc ccg gga ggc agc cac cac gcg 1356
Ala Leu Asn Asp Gly Val Ala Phe Gly Pro Gly Gly Ser His His Ala
340 345 350
cgg ctg aac ttc gcc acg tcc cgg gag atc ctg gag gag gcc tgc gac 1404
Arg Leu Asn Phe Ala Thr Ser Arg Glu Ile Leu Glu Glu Ala Cys Asp
355 360 365
cgg ctg ggg tct gca ttc gcc cgg tga tttccctacc ctggatttca 1451
Arg Leu Gly Ser Ala Phe Ala Arg
370 375
tgactgacga gcgatacgac gacccgtccg ccacgaccgg cccccagggt cccggactgt 1511
ccatgggtcc gctgggggtc ggcgccgaac cggagaagtc cccggtccgc ccccgtaccg 1571
acgccgaggg ctacctggtc gaaccggagt ccgagcagtt cgagaccccg accccggcgc 1631
ccggtgacgc cccctgggag cgccccgagc cggagtggta caaggaggcc gtgttctacg 1691
aggtcctggt gcgcgccttc tacgacccgg agggcaccgg 1731
<210> 26
<211> 376
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 26
Met Gln Phe Pro Thr Leu Asp Gln Leu Lys Asp Arg Arg Thr Arg Lys
1 5 10 15
Trp Thr Val Tyr Ala Asp Asp Val Leu Pro Leu Trp Ile Ala Glu Ser
20 25 30
Asp Phe Phe Thr Cys Pro Pro Val Lys Gln Ala Ile Ala Asp Ala Ile
35 40 45
Asp Arg Glu Ser Phe Gly Tyr Thr Pro Ala Thr Ser Asp Leu Pro Gln
50 55 60
Ala Val Ser Asp Phe Tyr Ala Ala Arg Tyr Gly Trp Arg Pro Asp Pro
65 70 75 80
Ala Leu Ile Val Ala Val Pro Asp Val Val Arg Gly Leu Ala Leu Ala
85 90 95
Ile Glu Tyr Met Thr Arg Pro Asp Ser Gly Val Ile Val Pro Val Pro
100 105 110
Ala Tyr Pro Pro Phe Leu Glu Leu Pro Glu Ala Thr Arg Arg Pro Met
115 120 125
His Gln Ile Asp Ala Tyr Gly Gly Ile Asp Leu Gly Asp Val Glu Lys
130 135 140
Gln Phe Ala Ala Gly Ala Gly Ser Ile Leu Leu Cys Ala Pro Asn Asn
145 150 155 160
Pro Leu Gly Tyr Thr Phe Asp Ala Glu Phe Leu Arg Glu Leu Cys Asp
165 170 175
Leu Ala Asp Arg Tyr Asp Ala Arg Val Leu Val Asp Glu Ile His Ala
180 185 190
Pro Leu Val Phe Asp Gly Thr His Val Cys Ala Ala Ala Val Ser Glu
195 200 205
Thr Ala Ala Arg Val Cys Val Thr Val Thr Ala Thr Ser Lys Ala Trp
210 215 220
Asn Ile Ala Gly Leu Lys Cys Ala Gln Met Ile Phe Ser Asn Pro Ala
225 230 235 240
Asp Met Asp Thr Trp Arg Ser Met Thr Gly Val Ala Lys Asp Gly Thr
245 250 255
Ser Thr Leu Gly Ile Trp Ala Ala Ile Ala Cys Tyr Arg Glu Gly Gly
260 265 270
Asp Cys Leu Asp Glu Gln Val Ala Tyr Leu Arg Glu Asn Arg Asp Trp
275 280 285
Leu Ala Glu Glu Leu Pro Arg Arg Val Pro Gly Leu Gln Val Ile Ser
290 295 300
Pro Ala Ala Thr Tyr Leu Met Trp Met Asp Phe Ser Gly Thr Ser Ile
305 310 315 320
Gly His Leu Glu Arg Pro Ala Val Trp Leu Arg Glu Asn Ala Lys Val
325 330 335
Ala Leu Asn Asp Gly Val Ala Phe Gly Pro Gly Gly Ser His His Ala
340 345 350
Arg Leu Asn Phe Ala Thr Ser Arg Glu Ile Leu Glu Glu Ala Cys Asp
355 360 365
Arg Leu Gly Ser Ala Phe Ala Arg
370 375
<210> 27
<211> 1632
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1332)
<223> ген asd
<400> 27
cggctggtcc aacgtgctct acgacgacca ggtgggcaag gtctccctcg tgggtgccgg 60
catgaagtcc cacccgggcg tcaccgccga cttcgccgag gcgctccgcg acgccggcgt 120
gaacatcgag ctaatctcca cctcggagat ccgcatctcg gtgctcaccc gcgaggccga 180
cctggacgcc gccgcccgtg ccctgcacga gaagttcgag ctcggcggcg acgccgaggc 240
caccgtctac gccggcaccg gccgctagac aatcccttcc acagaggagc tttcactctc 300
atg acc acc att gct gtc gtc ggt gcc acc ggc cag gtc ggc cgc gtg 348
Met Thr Thr Ile Ala Val Val Gly Ala Thr Gly Gln Val Gly Arg Val
1 5 10 15
atg cgc tcg atc ctg gag gag cgc aac ttc ccg gcc gac aag gtc cgt 396
Met Arg Ser Ile Leu Glu Glu Arg Asn Phe Pro Ala Asp Lys Val Arg
20 25 30
ttc ttc gcg tcc ccg cgc tcc gcg ggc acc gtc atc gag ttc cgc ggt 444
Phe Phe Ala Ser Pro Arg Ser Ala Gly Thr Val Ile Glu Phe Arg Gly
35 40 45
gag gac atc acc gtc gag gac ctc acc cag atc acc ccg gag tcc gtc 492
Glu Asp Ile Thr Val Glu Asp Leu Thr Gln Ile Thr Pro Glu Ser Val
50 55 60
aag gac gtc gac atc gcc ctc ttc tcc gcc ggt ggt tcc acc tcc cgt 540
Lys Asp Val Asp Ile Ala Leu Phe Ser Ala Gly Gly Ser Thr Ser Arg
65 70 75 80
gag tgg gcc ccg gtc ttc gcc gag gcc ggc gcg acc gtc atc gac aac 588
Glu Trp Ala Pro Val Phe Ala Glu Ala Gly Ala Thr Val Ile Asp Asn
85 90 95
tcc tcc gcc tgg cgc aag gac gac gag gtc ccg ctc gtc gtc gcc gag 636
Ser Ser Ala Trp Arg Lys Asp Asp Glu Val Pro Leu Val Val Ala Glu
100 105 110
gtc aac ggc gag gag gcg aag aac ccg tcc aag ggc atc gtc gcc aac 684
Val Asn Gly Glu Glu Ala Lys Asn Pro Ser Lys Gly Ile Val Ala Asn
115 120 125
ccg aac tgc acc acc atg gcc gcc atg ccg gtg ctc aag ccg ctg cac 732
Pro Asn Cys Thr Thr Met Ala Ala Met Pro Val Leu Lys Pro Leu His
130 135 140
gac gcc gcc ggc ctc atc cgc ctg cat gtc tcc tcc tac cag gcc gtc 780
Asp Ala Ala Gly Leu Ile Arg Leu His Val Ser Ser Tyr Gln Ala Val
145 150 155 160
tcc ggc tcc ggc ctc gcc ggt gtg gag acc ctg gcg aag cag acc gcc 828
Ser Gly Ser Gly Leu Ala Gly Val Glu Thr Leu Ala Lys Gln Thr Ala
165 170 175
gag ctc ggt gag gac aac gtc aag ctg gtc cat gac ggt tcc gtc gag 876
Glu Leu Gly Glu Asp Asn Val Lys Leu Val His Asp Gly Ser Val Glu
180 185 190
gcc ccc gcc gac ctg ggt ccc tac gtc gcc ccc atc gcg ttc aac gcc 924
Ala Pro Ala Asp Leu Gly Pro Tyr Val Ala Pro Ile Ala Phe Asn Ala
195 200 205
ctg ccg ctg gcc ggc gcg ctg gtc gat gac ggc tcc ctg gag acg gat 972
Leu Pro Leu Ala Gly Ala Leu Val Asp Asp Gly Ser Leu Glu Thr Asp
210 215 220
gag gag cag aag ctg cgc aac gag tcc cgc aag atc ctg gac atc ccg 1020
Glu Glu Gln Lys Leu Arg Asn Glu Ser Arg Lys Ile Leu Asp Ile Pro
225 230 235 240
ggt ctg cgc gtc gcc ggc acc tgc gtc cgc gtg ccg gtg ttc acc ggt 1068
Gly Leu Arg Val Ala Gly Thr Cys Val Arg Val Pro Val Phe Thr Gly
245 250 255
cac acc ctg acc atc cac gcc gag ttc gag cgt ccg atc acc gtc gac 1116
His Thr Leu Thr Ile His Ala Glu Phe Glu Arg Pro Ile Thr Val Asp
260 265 270
gag gcc aag gcc ctg ctc gcc gac gcc ccg ggt gtc gag ctt gtc gac 1164
Glu Ala Lys Ala Leu Leu Ala Asp Ala Pro Gly Val Glu Leu Val Asp
275 280 285
gtc ccg acc ccg ctg gag gcc gcc ggc aag gac acc tcc ctg gtc ggc 1212
Val Pro Thr Pro Leu Glu Ala Ala Gly Lys Asp Thr Ser Leu Val Gly
290 295 300
cgt atc cgc cag gac cag tcc gtc gac gac aac aag ggc ctc gtg ctc 1260
Arg Ile Arg Gln Asp Gln Ser Val Asp Asp Asn Lys Gly Leu Val Leu
305 310 315 320
gtc gtc tcc ggt gac aac ctg cgc aag ggc gcg gcg ctg aac acg gtg 1308
Val Val Ser Gly Asp Asn Leu Arg Lys Gly Ala Ala Leu Asn Thr Val
325 330 335
cag atc gcg gag ctg ctg gtg tag tccaccgcct ccactgatat cgggcccctc 1362
Gln Ile Ala Glu Leu Leu Val
340
accatgttgt ggtgaggggc ccgttcttgt cggtggtctg gtaggggagg ccttcattct 1422
gtccagacaa ttcttgtggt gtcctggggc gtcggcatgc ggcgtcgggc gggtatcggc 1482
gttcccgttc ctcgtttggc ggttcactgc cggacaatgg aggattttct caggaacagg 1542
gatccctgct gatctcgtat ttcgccccgg aactccgctg gacgctcagc ttgtccccag 1602
aattggggtc tttccgcagc gaattgtgag 1632
<210> 28
<211> 343
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 28
Met Thr Thr Ile Ala Val Val Gly Ala Thr Gly Gln Val Gly Arg Val
1 5 10 15
Met Arg Ser Ile Leu Glu Glu Arg Asn Phe Pro Ala Asp Lys Val Arg
20 25 30
Phe Phe Ala Ser Pro Arg Ser Ala Gly Thr Val Ile Glu Phe Arg Gly
35 40 45
Glu Asp Ile Thr Val Glu Asp Leu Thr Gln Ile Thr Pro Glu Ser Val
50 55 60
Lys Asp Val Asp Ile Ala Leu Phe Ser Ala Gly Gly Ser Thr Ser Arg
65 70 75 80
Glu Trp Ala Pro Val Phe Ala Glu Ala Gly Ala Thr Val Ile Asp Asn
85 90 95
Ser Ser Ala Trp Arg Lys Asp Asp Glu Val Pro Leu Val Val Ala Glu
100 105 110
Val Asn Gly Glu Glu Ala Lys Asn Pro Ser Lys Gly Ile Val Ala Asn
115 120 125
Pro Asn Cys Thr Thr Met Ala Ala Met Pro Val Leu Lys Pro Leu His
130 135 140
Asp Ala Ala Gly Leu Ile Arg Leu His Val Ser Ser Tyr Gln Ala Val
145 150 155 160
Ser Gly Ser Gly Leu Ala Gly Val Glu Thr Leu Ala Lys Gln Thr Ala
165 170 175
Glu Leu Gly Glu Asp Asn Val Lys Leu Val His Asp Gly Ser Val Glu
180 185 190
Ala Pro Ala Asp Leu Gly Pro Tyr Val Ala Pro Ile Ala Phe Asn Ala
195 200 205
Leu Pro Leu Ala Gly Ala Leu Val Asp Asp Gly Ser Leu Glu Thr Asp
210 215 220
Glu Glu Gln Lys Leu Arg Asn Glu Ser Arg Lys Ile Leu Asp Ile Pro
225 230 235 240
Gly Leu Arg Val Ala Gly Thr Cys Val Arg Val Pro Val Phe Thr Gly
245 250 255
His Thr Leu Thr Ile His Ala Glu Phe Glu Arg Pro Ile Thr Val Asp
260 265 270
Glu Ala Lys Ala Leu Leu Ala Asp Ala Pro Gly Val Glu Leu Val Asp
275 280 285
Val Pro Thr Pro Leu Glu Ala Ala Gly Lys Asp Thr Ser Leu Val Gly
290 295 300
Arg Ile Arg Gln Asp Gln Ser Val Asp Asp Asn Lys Gly Leu Val Leu
305 310 315 320
Val Val Ser Gly Asp Asn Leu Arg Lys Gly Ala Ala Leu Asn Thr Val
325 330 335
Gln Ile Ala Glu Leu Leu Val
340
<210> 29
<211> 1725
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1425)
<223> ген brnE
<400> 29
gatctcgttg gccttgcccg tggcaccgac ggcggtgagg gcgtcgagga cctcgcggga 60
ggcggcgtcg tcgagaagga cggaggccgg cagctccggg ccgtcctcac cgcagaggac 120
ggggacgagc agtgcgtcgg gggtcttcgg ggccttcttg gccagctcga cggcgggggt 180
ggtgccgcgg gcgggaaggg tgaaagtggg gttcgccatg gaaagtacct cctgagattg 240
acaagcgatg aacccagctt atagagatca cctctgggtc gtcggtgtag cgtgttcccc 300
atg acg tct ctc aat ttc acc gtg aac cgt acg agc acc ccg acg tcc 348
Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser
1 5 10 15
gat gag gcg cgc gag gag att ctg cag aat ccg cgg ttc ggc aag aac 396
Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn
20 25 30
ttc acc gat cac atg gtc acc atc gag tgg tcc gag gac aag ggc tgg 444
Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp
35 40 45
cac gac gcc cgc gtg cgt ccc tac gag gcc atc ccg atg gat ccc gcg 492
His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala
50 55 60
acc acg gtg ttc cac tac ggc cag gcg atc ttc gag ggc atc aag gcc 540
Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala
65 70 75 80
tac cgt cag ccg gac ggc tcc atc gcc acc ttc cgc ccg gag cgc aac 588
Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn
85 90 95
gcc gag cgc atg cag cgt tcc gcc gag cgc atg gcc atg ccg ccg ctg 636
Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu
100 105 110
ccg acc gag gac ttc ctc gag acg ctc cgc ctg ctt gtc gac gtc gac 684
Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp
115 120 125
cgt gac tgg gtc ccc gcc gcc ggc ggc gag gcc agc ctc tac ctg cgt 732
Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg
130 135 140
ccc ttc atg atc tcc acc gag gtc tcc ctg ggc gtc agc ccg gcc aac 780
Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn
145 150 155 160
cgg tac acc ttc ttc gtc atc gcc tcc ccg gtc ggg gcg tac ttc acc 828
Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr
165 170 175
ggt ggc gtc aag ccg gtc tcc gtg tgg ctg agc gag gac tac gtc cgt 876
Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg
180 185 190
gcc gcc ccg ggt ggc acc ggt gcc gcg aag ttc gcc ggc aac tac gcc 924
Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala
195 200 205
gcc tcc ctg ctc gcg cag gcg cag gcg gag gag aag ggc tgc gac cag 972
Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln
210 215 220
gtc gtg tgg ctc gac gcc atc gag cac acc tac atc gag gag atg ggt 1020
Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly
225 230 235 240
ggc atg aac ctg ttc ttc gtg tac ggc tcg ggt gac gac gtc acc gtg 1068
Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val
245 250 255
gcc acc ccc gag ctc tcc ggc tcc ctc ctg ccg ggc gtc acc cgc gac 1116
Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp
260 265 270
tcc ctc ctg cag gtc gcc cgc gat ctc ggc cac cgc acc gag gag cgg 1164
Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg
275 280 285
agg atc tcc aag ttc gac tgg cgc gac gac gtc gcc tcc ggt gcg atg 1212
Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met
290 295 300
acc gag gcc atg gcc tgc ggc aca gcc gcc gtg atc acc ccg gtc ggc 1260
Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly
305 310 315 320
cgg gtc ctg tcc aac gac ggc gag ttc cag atc aat gac aac cag ccg 1308
Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro
325 330 335
ggt gag gtc acc atg gcc atg cgc gag cgt ctc acc gac atc cag cgc 1356
Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg
340 345 350
ggc ctg tac gag gac acc cac ggc tgg gtg cac acc ctc gtc ccg gct 1404
Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala
355 360 365
gag gac cag ccg gcg gac tag tcggccagca gctcggcggc ggcccgcacc 1455
Glu Asp Gln Pro Ala Asp
370
agcgggagcg ccgctagcgc gccggtgccc tggccggcgt tcatgtccag ggcgacgaga 1515
ggcgtgagtt cgagggcctg cagcgcgatg aggtgcgcgg gctccggggt cagctgccct 1575
gcctggcacc agggggcggt gccgggggag agacgttccg cgaggtaggc ggcgacggtg 1635
acgggggcgc cgtcgagaag cacgggggtg cggcgtgccg cggcctgcgc gatgaaggcc 1695
accagtgcca ccaggtccgg ggaggcgatc 1725
<210> 30
<211> 374
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 30
Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser
1 5 10 15
Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn
20 25 30
Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp
35 40 45
His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala
50 55 60
Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala
65 70 75 80
Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn
85 90 95
Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu
100 105 110
Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp
115 120 125
Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg
130 135 140
Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn
145 150 155 160
Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr
165 170 175
Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg
180 185 190
Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala
195 200 205
Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln
210 215 220
Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly
225 230 235 240
Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val
245 250 255
Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp
260 265 270
Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg
275 280 285
Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met
290 295 300
Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly
305 310 315 320
Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro
325 330 335
Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg
340 345 350
Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala
355 360 365
Glu Asp Gln Pro Ala Asp
370
<210> 31
<211> 1296
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(996)
<223> ген brnF
<400> 31
cgacatggag acggccccgc agctcaaccg cctggccaac cgcctcgccc acgtcgacct 60
gcgcgccgat cccggtgaca tcgccgagct cgtcgagggc atgcgtctgt tcgccggtta 120
cgccgagtgg gcgcccggcc agctcgacgg ggagatcgac cgcggcgact ggttcgtcac 180
cccggcgctg cccaccgacg tcatcgtccc gggccgcacc gacctgtggg gcgacgtcat 240
gcgccgccag cagccgccgc tgccgctctt ctcgacgttc cccgccgacc tcatggacaa 300
ttg acc ccc gcg ttc cct gac gtc cgt gcc gga ctg cgg gac gcc tgg 348
Leu Thr Pro Ala Phe Pro Asp Val Arg Ala Gly Leu Arg Asp Ala Trp
1 5 10 15
gcc gtc gcg ctc ggg ctg gtc ccc ctg ggg ctg gcc ttc ggc ctg ctg 396
Ala Val Ala Leu Gly Leu Val Pro Leu Gly Leu Ala Phe Gly Leu Leu
20 25 30
atg acg cag gtg ggt ttc gcc tgg tgg tgg acg ccg gtc ttc tcg gtg 444
Met Thr Gln Val Gly Phe Ala Trp Trp Trp Thr Pro Val Phe Ser Val
35 40 45
ctc atc tac gcc ggg tcc atg gag ttc ctg gcg gtc aac ctg gtg ctc 492
Leu Ile Tyr Ala Gly Ser Met Glu Phe Leu Ala Val Asn Leu Val Leu
50 55 60
gcc ggc gtc ggc ccc ctg tcg acg gcg ctg acc gcc ttc atg gtc aac 540
Ala Gly Val Gly Pro Leu Ser Thr Ala Leu Thr Ala Phe Met Val Asn
65 70 75 80
ttc cgg cac atc ttc tac ggc ctc acc ttc ccc cgg cac ctc gtc cgg 588
Phe Arg His Ile Phe Tyr Gly Leu Thr Phe Pro Arg His Leu Val Arg
85 90 95
ggc cgg gcc ggc cgt gct tac tcc acg tac tcg ctg acc gac gag tcc 636
Gly Arg Ala Gly Arg Ala Tyr Ser Thr Tyr Ser Leu Thr Asp Glu Ser
100 105 110
tac gcc atc gcc tcc gcc cac cct ccg cgc tcc ggt acg cag gtg ctg 684
Tyr Ala Ile Ala Ser Ala His Pro Pro Arg Ser Gly Thr Gln Val Leu
115 120 125
acg atc cag ctc ttc tgc cag gcg atg tgg gtg gtg ccc ggc atc atc 732
Thr Ile Gln Leu Phe Cys Gln Ala Met Trp Val Val Pro Gly Ile Ile
130 135 140
ggg gcg ctc gtc ggt gag gtc atc ccg gcg ggc atc cag ggc atg gag 780
Gly Ala Leu Val Gly Glu Val Ile Pro Ala Gly Ile Gln Gly Met Glu
145 150 155 160
ttc gca ctc gtc gca ctg ttc gtg gtg ctg gcg tgg gag acc ttc cgg 828
Phe Ala Leu Val Ala Leu Phe Val Val Leu Ala Trp Glu Thr Phe Arg
165 170 175
ggc agc cgg gac ctc tcc ctg ccg ctg ctc gcc ggt ggc ctg gca ctc 876
Gly Ser Arg Asp Leu Ser Leu Pro Leu Leu Ala Gly Gly Leu Ala Leu
180 185 190
gtt gcc ggc ctg gtg ctc ccg ggg cag atg ctc atg gtg gcg ctg agc 924
Val Ala Gly Leu Val Leu Pro Gly Gln Met Leu Met Val Ala Leu Ser
195 200 205
gcc tac ttc ctc gtc ctc atc ggc cgg cac ctc tcc ccc gcc ctg gac 972
Ala Tyr Phe Leu Val Leu Ile Gly Arg His Leu Ser Pro Ala Leu Asp
210 215 220
cgt cgc ctg gag tgg agg gtc tga tgtacgggct tcccgagggg gtcaccctca 1026
Arg Arg Leu Glu Trp Arg Val
225 230
cccagacgtt ggcggtcctg ctgcccatcg gggtggtcac ggtggcgctg cgctggctgc 1086
ccttcgcctt tgtcagggcg ctgcgcgaca accagttctt cggtctgctc gcccggatga 1146
tgccggtggg agtgatgacc gtgctggtgg tgtacacgct cctcgggcag cgttcggcgc 1206
ccggtggcct ggcggcggcc ctcatcgggg tcgcggtgac cctggggctg cacgcctggc 1266
ggcgggactc tgggctctcg atcctcggag 1296
<210> 32
<211> 231
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 32
Leu Thr Pro Ala Phe Pro Asp Val Arg Ala Gly Leu Arg Asp Ala Trp
1 5 10 15
Ala Val Ala Leu Gly Leu Val Pro Leu Gly Leu Ala Phe Gly Leu Leu
20 25 30
Met Thr Gln Val Gly Phe Ala Trp Trp Trp Thr Pro Val Phe Ser Val
35 40 45
Leu Ile Tyr Ala Gly Ser Met Glu Phe Leu Ala Val Asn Leu Val Leu
50 55 60
Ala Gly Val Gly Pro Leu Ser Thr Ala Leu Thr Ala Phe Met Val Asn
65 70 75 80
Phe Arg His Ile Phe Tyr Gly Leu Thr Phe Pro Arg His Leu Val Arg
85 90 95
Gly Arg Ala Gly Arg Ala Tyr Ser Thr Tyr Ser Leu Thr Asp Glu Ser
100 105 110
Tyr Ala Ile Ala Ser Ala His Pro Pro Arg Ser Gly Thr Gln Val Leu
115 120 125
Thr Ile Gln Leu Phe Cys Gln Ala Met Trp Val Val Pro Gly Ile Ile
130 135 140
Gly Ala Leu Val Gly Glu Val Ile Pro Ala Gly Ile Gln Gly Met Glu
145 150 155 160
Phe Ala Leu Val Ala Leu Phe Val Val Leu Ala Trp Glu Thr Phe Arg
165 170 175
Gly Ser Arg Asp Leu Ser Leu Pro Leu Leu Ala Gly Gly Leu Ala Leu
180 185 190
Val Ala Gly Leu Val Leu Pro Gly Gln Met Leu Met Val Ala Leu Ser
195 200 205
Ala Tyr Phe Leu Val Leu Ile Gly Arg His Leu Ser Pro Ala Leu Asp
210 215 220
Arg Arg Leu Glu Trp Arg Val
225 230
<210> 33
<211> 1200
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(900)
<223> ген cysE
<400> 33
tggaccgcaa ggtcctcgac gacgtcatca ccgtcaccaa cgaggacgcc atcgagacct 60
cccgtcagct ggcggtcaag gacggcatcc tgggcggtat ctcctccggc ggcaacgtct 120
gggccgcgct ggagctggcg aagaaggagg agaacgcggg caagaccatc gtcaccgtcg 180
tcaccgacta cggcgagcgt tacgtctcca ccgtcctgta cgaggacatt cgcgactagg 240
aaacggctta ggcccccgca gcgcccacca ggcgacgggc aagcaagtag actctgggcc 300
atg ctg ctg ttg aac atc gcg aag atg gtc cgg gag gac ctg cgc aac 348
Met Leu Leu Leu Asn Ile Ala Lys Met Val Arg Glu Asp Leu Arg Asn
1 5 10 15
gcc cgc gac cac gat ccc gcc gcc cgc ggc gac atc gag aac gcc gtc 396
Ala Arg Asp His Asp Pro Ala Ala Arg Gly Asp Ile Glu Asn Ala Val
20 25 30
gtc tac tcg ggc ctg cac gcc atc tgg gca cac cgt gtc tcc cac tgg 444
Val Tyr Ser Gly Leu His Ala Ile Trp Ala His Arg Val Ser His Trp
35 40 45
atg tgg gag cgc ggt ctc cgc ggc ccc gcg cga atc ctc gcg cag ctc 492
Met Trp Glu Arg Gly Leu Arg Gly Pro Ala Arg Ile Leu Ala Gln Leu
50 55 60
aac cgg ttc ttc acg ggc atc gag atc cac ccc ggt gcc acc atc ggc 540
Asn Arg Phe Phe Thr Gly Ile Glu Ile His Pro Gly Ala Thr Ile Gly
65 70 75 80
cgc cgt ttc ttc atc gac cac ggc atg ggc atc gtc atc ggc gag acc 588
Arg Arg Phe Phe Ile Asp His Gly Met Gly Ile Val Ile Gly Glu Thr
85 90 95
acc gag atc ggc gac ggc gtc atg ctc tac cac ggc gtc acc ctg ggt 636
Thr Glu Ile Gly Asp Gly Val Met Leu Tyr His Gly Val Thr Leu Gly
100 105 110
ggt cag gtg ctc acg cag acc aag cgc cac ccg acc atc gag gac aac 684
Gly Gln Val Leu Thr Gln Thr Lys Arg His Pro Thr Ile Glu Asp Asn
115 120 125
gtc acc atc ggt gcc ggc gcc aag gtg ctc ggc ccg atc acc atc ggc 732
Val Thr Ile Gly Ala Gly Ala Lys Val Leu Gly Pro Ile Thr Ile Gly
130 135 140
aag ggc tcc gcg atc ggc gcc aac gcc gtg gtg acc aag gac gtc ccg 780
Lys Gly Ser Ala Ile Gly Ala Asn Ala Val Val Thr Lys Asp Val Pro
145 150 155 160
gcg gac cac atc gcc acg ggc atc ccg gcg aag aac cgc ccg cgc cgc 828
Ala Asp His Ile Ala Thr Gly Ile Pro Ala Lys Asn Arg Pro Arg Arg
165 170 175
cag tcc gag cgc atc aag ctc gtc gat ccg gac tac tac acc tcc aac 876
Gln Ser Glu Arg Ile Lys Leu Val Asp Pro Asp Tyr Tyr Thr Ser Asn
180 185 190
gat ccg gtc gac tac agc atc tag acacaaggaa ggccccggtt tcccggggcc 930
Asp Pro Val Asp Tyr Ser Ile
195
tttttgtgct tcacgacgtc ggcgcgtgcc cgacgtcacc cgacgaggtc gcggtactcc 990
tcgttcttgc tgatgaagtg ctcgaccgcg gaacagctcg ggcgcacccg cgccccctcc 1050
tcccgggccc agtcgagggc ctcgcggatg agcggggtgg acagcgcacg tccgcggtag 1110
gcctggtcca ccacggtgtg gtggaagtcc agtaggccac cgtggcggac gtaggaggcg 1170
tagccgacct cccggtcgtc cacggagatg 1200
<210> 34
<211> 199
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 34
Met Leu Leu Leu Asn Ile Ala Lys Met Val Arg Glu Asp Leu Arg Asn
1 5 10 15
Ala Arg Asp His Asp Pro Ala Ala Arg Gly Asp Ile Glu Asn Ala Val
20 25 30
Val Tyr Ser Gly Leu His Ala Ile Trp Ala His Arg Val Ser His Trp
35 40 45
Met Trp Glu Arg Gly Leu Arg Gly Pro Ala Arg Ile Leu Ala Gln Leu
50 55 60
Asn Arg Phe Phe Thr Gly Ile Glu Ile His Pro Gly Ala Thr Ile Gly
65 70 75 80
Arg Arg Phe Phe Ile Asp His Gly Met Gly Ile Val Ile Gly Glu Thr
85 90 95
Thr Glu Ile Gly Asp Gly Val Met Leu Tyr His Gly Val Thr Leu Gly
100 105 110
Gly Gln Val Leu Thr Gln Thr Lys Arg His Pro Thr Ile Glu Asp Asn
115 120 125
Val Thr Ile Gly Ala Gly Ala Lys Val Leu Gly Pro Ile Thr Ile Gly
130 135 140
Lys Gly Ser Ala Ile Gly Ala Asn Ala Val Val Thr Lys Asp Val Pro
145 150 155 160
Ala Asp His Ile Ala Thr Gly Ile Pro Ala Lys Asn Arg Pro Arg Arg
165 170 175
Gln Ser Glu Arg Ile Lys Leu Val Asp Pro Asp Tyr Tyr Thr Ser Asn
180 185 190
Asp Pro Val Asp Tyr Ser Ile
195
<210> 35
<211> 1536
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1236)
<223> ген cysK
<400> 35
cctccgggta cacccgtaca ggtgcacccg atgggcacat gaacgggggt aactttcctt 60
tacagatggg cattataacg cgacttcggg ggtaaacgga atcgaatccg ccgtaacttg 120
cgcattcaag accgttctga tcggaacaac ggcacccact gaaggggtga tatcccgcgc 180
cggagaccac ccccgcaacc ttggagaaac catgtggtct gccagggaca taccatcctg 240
tctactcaca tggtgtacgc tgacaccacc aacgtcagac gaagcaagga agcagtcacc 300
atg agc aag atc tac gac aac atc ctc gac acc atc gga aac acc ccg 348
Met Ser Lys Ile Tyr Asp Asn Ile Leu Asp Thr Ile Gly Asn Thr Pro
1 5 10 15
ctg gtg cgc ctc aac ggc ctc acc gag ggc ctc cag gcg gag gtg ctc 396
Leu Val Arg Leu Asn Gly Leu Thr Glu Gly Leu Gln Ala Glu Val Leu
20 25 30
gtg aag gtc gag tcc ttc aac ccg gcg aac tcc gtc aag gac cgc atc 444
Val Lys Val Glu Ser Phe Asn Pro Ala Asn Ser Val Lys Asp Arg Ile
35 40 45
ggc aag gcg atc atc gac gcc gcc gag gcc gac ggg tcc ctc cag ccg 492
Gly Lys Ala Ile Ile Asp Ala Ala Glu Ala Asp Gly Ser Leu Gln Pro
50 55 60
ggc ggc acc atc gtg gag gcc acc tcc ggc aac acc ggc atc gcc ctc 540
Gly Gly Thr Ile Val Glu Ala Thr Ser Gly Asn Thr Gly Ile Ala Leu
65 70 75 80
gcc ctc gtg ggc gcg gcc cgc ggc tac aac gtg atc ctc acc atg ccg 588
Ala Leu Val Gly Ala Ala Arg Gly Tyr Asn Val Ile Leu Thr Met Pro
85 90 95
gag acc atg tcc aac gag cgt cgc gtc atg ctc cgc gcc tac ggc gct 636
Glu Thr Met Ser Asn Glu Arg Arg Val Met Leu Arg Ala Tyr Gly Ala
100 105 110
cag atc gtc ctg acc ccg ggc gcg gcc ggc atg cag ggc gcc gtc gac 684
Gln Ile Val Leu Thr Pro Gly Ala Ala Gly Met Gln Gly Ala Val Asp
115 120 125
aag gcg aac gag atc gtc gcc gcc gag gac aac gcc atc ctc gcg aac 732
Lys Ala Asn Glu Ile Val Ala Ala Glu Asp Asn Ala Ile Leu Ala Asn
130 135 140
cag ttc gcg cac ccg gcg aac ctg cgg atc cac cgc gag acc acc gcc 780
Gln Phe Ala His Pro Ala Asn Leu Arg Ile His Arg Glu Thr Thr Ala
145 150 155 160
gag gag atc tgg aac gac acc gac ggc gag gtc gac atc ttc gtc gcg 828
Glu Glu Ile Trp Asn Asp Thr Asp Gly Glu Val Asp Ile Phe Val Ala
165 170 175
ggc atc ggc acg ggc ggc acc gtc tcc ggc gtc ggc cag gtg ctc aag 876
Gly Ile Gly Thr Gly Gly Thr Val Ser Gly Val Gly Gln Val Leu Lys
180 185 190
gag cgc aag ccg gag gtc cag atc tac gcc gtc gag ccg gcc gcg tcc 924
Glu Arg Lys Pro Glu Val Gln Ile Tyr Ala Val Glu Pro Ala Ala Ser
195 200 205
ccg ctg ctg tcc gcc ggc aag gcg ggc ccg cac aag atc cag ggc atc 972
Pro Leu Leu Ser Ala Gly Lys Ala Gly Pro His Lys Ile Gln Gly Ile
210 215 220
ggc gcc aac ttc atc ccg gag gtc ctg gac cgc aag gtc ctc gac gac 1020
Gly Ala Asn Phe Ile Pro Glu Val Leu Asp Arg Lys Val Leu Asp Asp
225 230 235 240
gtc atc acc gtc acc aac gag gac gcc atc gag acc tcc cgt cag ctg 1068
Val Ile Thr Val Thr Asn Glu Asp Ala Ile Glu Thr Ser Arg Gln Leu
245 250 255
gcg gtc aag gac ggc atc ctg ggc ggt atc tcc tcc ggc ggc aac gtc 1116
Ala Val Lys Asp Gly Ile Leu Gly Gly Ile Ser Ser Gly Gly Asn Val
260 265 270
tgg gcc gcg ctg gag ctg gcg aag aag gag gag aac gcg ggc aag acc 1164
Trp Ala Ala Leu Glu Leu Ala Lys Lys Glu Glu Asn Ala Gly Lys Thr
275 280 285
atc gtc acc gtc gtc acc gac tac ggc gag cgt tac gtc tcc acc gtc 1212
Ile Val Thr Val Val Thr Asp Tyr Gly Glu Arg Tyr Val Ser Thr Val
290 295 300
ctg tac gag gac att cgc gac tag gaaacggctt aggcccccgc agcgcccacc 1266
Leu Tyr Glu Asp Ile Arg Asp
305 310
aggcgacggg caagcaagta gactctgggc catgctgctg ttgaacatcg cgaagatggt 1326
ccgggaggac ctgcgcaacg cccgcgacca cgatcccgcc gcccgcggcg acatcgagaa 1386
cgccgtcgtc tactcgggcc tgcacgccat ctgggcacac cgtgtctccc actggatgtg 1446
ggagcgcggt ctccgcggcc ccgcgcgaat cctcgcgcag ctcaaccggt tcttcacggg 1506
catcgagatc caccccggtg ccaccatcgg 1536
<210> 36
<211> 311
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 36
Met Ser Lys Ile Tyr Asp Asn Ile Leu Asp Thr Ile Gly Asn Thr Pro
1 5 10 15
Leu Val Arg Leu Asn Gly Leu Thr Glu Gly Leu Gln Ala Glu Val Leu
20 25 30
Val Lys Val Glu Ser Phe Asn Pro Ala Asn Ser Val Lys Asp Arg Ile
35 40 45
Gly Lys Ala Ile Ile Asp Ala Ala Glu Ala Asp Gly Ser Leu Gln Pro
50 55 60
Gly Gly Thr Ile Val Glu Ala Thr Ser Gly Asn Thr Gly Ile Ala Leu
65 70 75 80
Ala Leu Val Gly Ala Ala Arg Gly Tyr Asn Val Ile Leu Thr Met Pro
85 90 95
Glu Thr Met Ser Asn Glu Arg Arg Val Met Leu Arg Ala Tyr Gly Ala
100 105 110
Gln Ile Val Leu Thr Pro Gly Ala Ala Gly Met Gln Gly Ala Val Asp
115 120 125
Lys Ala Asn Glu Ile Val Ala Ala Glu Asp Asn Ala Ile Leu Ala Asn
130 135 140
Gln Phe Ala His Pro Ala Asn Leu Arg Ile His Arg Glu Thr Thr Ala
145 150 155 160
Glu Glu Ile Trp Asn Asp Thr Asp Gly Glu Val Asp Ile Phe Val Ala
165 170 175
Gly Ile Gly Thr Gly Gly Thr Val Ser Gly Val Gly Gln Val Leu Lys
180 185 190
Glu Arg Lys Pro Glu Val Gln Ile Tyr Ala Val Glu Pro Ala Ala Ser
195 200 205
Pro Leu Leu Ser Ala Gly Lys Ala Gly Pro His Lys Ile Gln Gly Ile
210 215 220
Gly Ala Asn Phe Ile Pro Glu Val Leu Asp Arg Lys Val Leu Asp Asp
225 230 235 240
Val Ile Thr Val Thr Asn Glu Asp Ala Ile Glu Thr Ser Arg Gln Leu
245 250 255
Ala Val Lys Asp Gly Ile Leu Gly Gly Ile Ser Ser Gly Gly Asn Val
260 265 270
Trp Ala Ala Leu Glu Leu Ala Lys Lys Glu Glu Asn Ala Gly Lys Thr
275 280 285
Ile Val Thr Val Val Thr Asp Tyr Gly Glu Arg Tyr Val Ser Thr Val
290 295 300
Leu Tyr Glu Asp Ile Arg Asp
305 310
<210> 37
<211> 999
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(699)
<223> ген gcvH
<400> 37
cagggagccg accagcgcca tcgtcgggct ccggggtctg gggcgccgcg cggcccgcga 60
gggcgccgag gtctacctcg acggcgaaca ggtcggcgtg gtcacctccg ggcagccggc 120
gccggtgctc ggctacccga tcgcactcgc ccgcctgcgc cccgacgtca ccgcggccgg 180
cacggcactg gaggtggaca tccgtgggag gcggcacccc ttcgagatcg tcgacctgcc 240
cttctaccgc cgcgccgcac gataacctga acccaccacc aacggaaagg ctcccccacc 300
atg gcc acc gag cac tcc ctg ccc ggg aac tac tcc tac tcc gag gac 348
Met Ala Thr Glu His Ser Leu Pro Gly Asn Tyr Ser Tyr Ser Glu Asp
1 5 10 15
cac gag tgg atc gac gcg acg ccc gag gac gcc gtc ggc gcg acc gtg 396
His Glu Trp Ile Asp Ala Thr Pro Glu Asp Ala Val Gly Ala Thr Val
20 25 30
aag gtc ggc atc acc tcc gtg gcc acc gaa cgc ctc ggc gag gtc gtc 444
Lys Val Gly Ile Thr Ser Val Ala Thr Glu Arg Leu Gly Glu Val Val
35 40 45
tac gcg gag ctc ccg cag gtc ggt gac gcc gtc acc gcc ggt gaa ccg 492
Tyr Ala Glu Leu Pro Gln Val Gly Asp Ala Val Thr Ala Gly Glu Pro
50 55 60
tgc ggc gag gtc gag tcc acg aag tcg gtc tcc gac ctc tac gcg ccg 540
Cys Gly Glu Val Glu Ser Thr Lys Ser Val Ser Asp Leu Tyr Ala Pro
65 70 75 80
gtc acg ggc acc gtc acc gag gtc aac gac gcg gtc cac gac gac tac 588
Val Thr Gly Thr Val Thr Glu Val Asn Asp Ala Val His Asp Asp Tyr
85 90 95
gcc gtg atc aac gac gac ccc ttc ggc gcg ggc tgg ctc ttc gcc gtc 636
Ala Val Ile Asn Asp Asp Pro Phe Gly Ala Gly Trp Leu Phe Ala Val
100 105 110
gag gtc gac gag gtc gga ccg ctc atg acc gcc gcc gag tac gcg gag 684
Glu Val Asp Glu Val Gly Pro Leu Met Thr Ala Ala Glu Tyr Ala Glu
115 120 125
gcc aac ggc gtg tga ccgcccggga ccggtaccct ggtcccatca tgactgcacc 739
Ala Asn Gly Val
130
ccgtgagccc ttcttccctg cggaccgttc catccgtgcc tccggcgagc ccccggagat 799
ccgttatctc ggcgtcgtcg actaccagga ggcctggaat ctgcaggccg agctggcgtc 859
gcaacgcgcg gccggggaga tcggtgacca gctcctcatc ctcgagcacc cgagcgtgta 919
caccgccggc aagcgcacgc agccggagga ccgccccacc aacggcctgc cggtcatcga 979
cgtcgaccgg ggtggccgca 999
<210> 38
<211> 132
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 38
Met Ala Thr Glu His Ser Leu Pro Gly Asn Tyr Ser Tyr Ser Glu Asp
1 5 10 15
His Glu Trp Ile Asp Ala Thr Pro Glu Asp Ala Val Gly Ala Thr Val
20 25 30
Lys Val Gly Ile Thr Ser Val Ala Thr Glu Arg Leu Gly Glu Val Val
35 40 45
Tyr Ala Glu Leu Pro Gln Val Gly Asp Ala Val Thr Ala Gly Glu Pro
50 55 60
Cys Gly Glu Val Glu Ser Thr Lys Ser Val Ser Asp Leu Tyr Ala Pro
65 70 75 80
Val Thr Gly Thr Val Thr Glu Val Asn Asp Ala Val His Asp Asp Tyr
85 90 95
Ala Val Ile Asn Asp Asp Pro Phe Gly Ala Gly Trp Leu Phe Ala Val
100 105 110
Glu Val Asp Glu Val Gly Pro Leu Met Thr Ala Ala Glu Tyr Ala Glu
115 120 125
Ala Asn Gly Val
130
<210> 39
<211> 3462
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(3162)
<223> ген gcvP
<400> 39
ctgggcaccg cggccatcga gaagcgtccg gtcgtggtga ccgatgacgg cgtcgactcg 60
atcgccatcc gccagatgtg ctacctgccg ttcacctacg accaccaggt catcgacggt 120
gccgacgccg gtcgcttcat caccaccatc aaggaccgcc tggagaccgc ggacttccag 180
tccgacctcg gcctctaagc tctcccgctg agagagaccg ccacctgacc ttcccggtcg 240
ggtggcggtt ttcttcttat aatcggaatc atccggtccc ccatcgagga gcgattccgc 300
atg gcc ctg cac ccg atc acc gat tcc gcc tcc tac ctg gcc cga cac 348
Met Ala Leu His Pro Ile Thr Asp Ser Ala Ser Tyr Leu Ala Arg His
1 5 10 15
ctc ggc ccc gac acc gcg gag gag cag gtc atg ctg ggg gcg ctg ggc 396
Leu Gly Pro Asp Thr Ala Glu Glu Gln Val Met Leu Gly Ala Leu Gly
20 25 30
tac ggt tcc ctc gcc gag ctt gtc gac gcc gcc ctg ccc acc gac atc 444
Tyr Gly Ser Leu Ala Glu Leu Val Asp Ala Ala Leu Pro Thr Asp Ile
35 40 45
cgc ctc acc gaa cct ctc ccg cgg cac gcg ccc ctc tcg gag acg gag 492
Arg Leu Thr Glu Pro Leu Pro Arg His Ala Pro Leu Ser Glu Thr Glu
50 55 60
gcg cag gcg cga ctg cgg gag tac gcc tcg cgg aac acg gtg ctc cgt 540
Ala Gln Ala Arg Leu Arg Glu Tyr Ala Ser Arg Asn Thr Val Leu Arg
65 70 75 80
tcc ttt tac ggg cag ggt ttc tcc gac acg gtc act ccc ccg gtg atc 588
Ser Phe Tyr Gly Gln Gly Phe Ser Asp Thr Val Thr Pro Pro Val Ile
85 90 95
cgc cgc ggg gtg ctc gag gcc ccg ggc tgg tac acc gcg tac acg ccg 636
Arg Arg Gly Val Leu Glu Ala Pro Gly Trp Tyr Thr Ala Tyr Thr Pro
100 105 110
tac cag ccg gag atc tcg cag ggc cgc ctc gag gcg ctg ctc aac ttc 684
Tyr Gln Pro Glu Ile Ser Gln Gly Arg Leu Glu Ala Leu Leu Asn Phe
115 120 125
cag acg atg gtc tcc gag ctc acc ggc ctg ccg gtg gcg aac gcc tcg 732
Gln Thr Met Val Ser Glu Leu Thr Gly Leu Pro Val Ala Asn Ala Ser
130 135 140
ctc ctc gac gag gct tcc gcc acc gcc gaa gcc gtc ggc ctc atg tcg 780
Leu Leu Asp Glu Ala Ser Ala Thr Ala Glu Ala Val Gly Leu Met Ser
145 150 155 160
cgg gcg cag agg aag ggg cgg cgg gtg ctt ctc gac gcc cgc ctc cac 828
Arg Ala Gln Arg Lys Gly Arg Arg Val Leu Leu Asp Ala Arg Leu His
165 170 175
ccc cag gtc ctc gcc gtc gct gcg gag cgg gcc cgg gcg atc gac ctg 876
Pro Gln Val Leu Ala Val Ala Ala Glu Arg Ala Arg Ala Ile Asp Leu
180 185 190
gag gtc gcc gtc gag gac ctg tcc ggc ccg ctg gtg ggt gag gat ctc 924
Glu Val Ala Val Glu Asp Leu Ser Gly Pro Leu Val Gly Glu Asp Leu
195 200 205
tgc ggc gtc gtc atc gcc tac ccc ggc acg gag ggc gac atc gcc gat 972
Cys Gly Val Val Ile Ala Tyr Pro Gly Thr Glu Gly Asp Ile Ala Asp
210 215 220
ccc cgc ccg gtc atc gag gcc gtg cac gca cgc ggg ggt ctg gcc acc 1020
Pro Arg Pro Val Ile Glu Ala Val His Ala Arg Gly Gly Leu Ala Thr
225 230 235 240
gtg gtc acc gat ccc ctg tcg ctg ctc ctg ctc gag tcg ccg ggt gag 1068
Val Val Thr Asp Pro Leu Ser Leu Leu Leu Leu Glu Ser Pro Gly Glu
245 250 255
atg ggg gcg gac atc gcg gtg ggc tcc tcg cag cgt ttc ggc gtg ccc 1116
Met Gly Ala Asp Ile Ala Val Gly Ser Ser Gln Arg Phe Gly Val Pro
260 265 270
ctg ttc tac ggt ggc ccg cac gcc gcg tac atg gcc gtc agt gac gcc 1164
Leu Phe Tyr Gly Gly Pro His Ala Ala Tyr Met Ala Val Ser Asp Ala
275 280 285
ctc aag cgc cag atc ccc ggc cgc ctg gtg ggc gtg tcc gtc gac gcc 1212
Leu Lys Arg Gln Ile Pro Gly Arg Leu Val Gly Val Ser Val Asp Ala
290 295 300
gag ggc gcc ccc gcc tac cgg ctc gcg ctg cag acc cgg gag cag cac 1260
Glu Gly Ala Pro Ala Tyr Arg Leu Ala Leu Gln Thr Arg Glu Gln His
305 310 315 320
atc cgt cgc gag cgg gcg acg tcg aac atc tgc acc gcg cag gcg ctg 1308
Ile Arg Arg Glu Arg Ala Thr Ser Asn Ile Cys Thr Ala Gln Ala Leu
325 330 335
ctg gcg gtc acc gcc ggc atg tac gcc gtc tac cac ggt ccc gag ggg 1356
Leu Ala Val Thr Ala Gly Met Tyr Ala Val Tyr His Gly Pro Glu Gly
340 345 350
ttg cgt gcc atc gcg gag cgc gtg cac ggt ctg gcc tgt gac ttc gcg 1404
Leu Arg Ala Ile Ala Glu Arg Val His Gly Leu Ala Cys Asp Phe Ala
355 360 365
gcg gcc gtc cgc gcc gcc ggc ggg acg gtg ctg cac gac gcc ttc ttc 1452
Ala Ala Val Arg Ala Ala Gly Gly Thr Val Leu His Asp Ala Phe Phe
370 375 380
gac acc gtc gcg gtg cgg gtg gag gct gca cga ccg atc gtc ggc acg 1500
Asp Thr Val Ala Val Arg Val Glu Ala Ala Arg Pro Ile Val Gly Thr
385 390 395 400
ctc gcg gag gcg ggc tac ctc gtg cgt gcc gtg gac ggc acg acc gtc 1548
Leu Ala Glu Ala Gly Tyr Leu Val Arg Ala Val Asp Gly Thr Thr Val
405 410 415
ggc gtg agc ttc ggt gag tcg gcg gag gag ggg gac gtc gag aag ctc 1596
Gly Val Ser Phe Gly Glu Ser Ala Glu Glu Gly Asp Val Glu Lys Leu
420 425 430
gcc gcc gtc ttc ggc gcc tcc ctg acg gcg ggc acc cgc tgc atc ccc 1644
Ala Ala Val Phe Gly Ala Ser Leu Thr Ala Gly Thr Arg Cys Ile Pro
435 440 445
gcg tcc gtg gcg cgg cag acg ccg acg ctg acg cac ccg gtc ttc tcc 1692
Ala Ser Val Ala Arg Gln Thr Pro Thr Leu Thr His Pro Val Phe Ser
450 455 460
acg ctc cac tcc gag acg cag atg atg cgc tac ctg cgc acg ctc tcg 1740
Thr Leu His Ser Glu Thr Gln Met Met Arg Tyr Leu Arg Thr Leu Ser
465 470 475 480
gac cgg gat ctc gcg ctc gac cgc acg atg atc ccg ctg ggt tcg tgc 1788
Asp Arg Asp Leu Ala Leu Asp Arg Thr Met Ile Pro Leu Gly Ser Cys
485 490 495
acg atg aag ctc aac ccg acc gcc ggg ctg gag acg atc acg tgg ccg 1836
Thr Met Lys Leu Asn Pro Thr Ala Gly Leu Glu Thr Ile Thr Trp Pro
500 505 510
gag ttc gcc gac atg cac ccc ttc gcc ccc gac gac cag gcc gcg ggc 1884
Glu Phe Ala Asp Met His Pro Phe Ala Pro Asp Asp Gln Ala Ala Gly
515 520 525
tgg ctg gcg ctc atc gcg gag ctg gag ggc tgg ctc gcg gag ctc acc 1932
Trp Leu Ala Leu Ile Ala Glu Leu Glu Gly Trp Leu Ala Glu Leu Thr
530 535 540
gga tac gcg aag gtg tcc gtg cag ccc aac gcg ggt tcc cag ggg gag 1980
Gly Tyr Ala Lys Val Ser Val Gln Pro Asn Ala Gly Ser Gln Gly Glu
545 550 555 560
ctg gcc gga ctg ctg gcc atc cgc cgc tac cac gtg gcc aac ggg gac 2028
Leu Ala Gly Leu Leu Ala Ile Arg Arg Tyr His Val Ala Asn Gly Asp
565 570 575
atg gac cgt gat atc tgc ctg gtc ccg gcc tcg gcg cac ggc acg aac 2076
Met Asp Arg Asp Ile Cys Leu Val Pro Ala Ser Ala His Gly Thr Asn
580 585 590
gcg gcg tcg gcg acg ctg gcg cac ctg cgg gtc gtg gtg gtc ggc acc 2124
Ala Ala Ser Ala Thr Leu Ala His Leu Arg Val Val Val Val Gly Thr
595 600 605
gcc ggg gac ggc tcc atc gac atg gac gac ctc gac gcg aaa ctc gcc 2172
Ala Gly Asp Gly Ser Ile Asp Met Asp Asp Leu Asp Ala Lys Leu Ala
610 615 620
gag cac ggc ccg cag gtg gcc gcg atc atg atc acc tac ccc tcc acg 2220
Glu His Gly Pro Gln Val Ala Ala Ile Met Ile Thr Tyr Pro Ser Thr
625 630 635 640
cac ggc gtc ttc gag gac acc gtg cgc gag gtg tgc gac agg gtg cac 2268
His Gly Val Phe Glu Asp Thr Val Arg Glu Val Cys Asp Arg Val His
645 650 655
gcg gtc ggc ggg cag gtc tac atc gac ggc gcg aac ctc aac gcc ctg 2316
Ala Val Gly Gly Gln Val Tyr Ile Asp Gly Ala Asn Leu Asn Ala Leu
660 665 670
acc ggc ctg gcg ctg ctg ggc cgc atc ggc ggc gac gtc tcc cac ctc 2364
Thr Gly Leu Ala Leu Leu Gly Arg Ile Gly Gly Asp Val Ser His Leu
675 680 685
aac ctg cac aag acg ttc acc atc ccg cac ggc ggc ggt ggc ccg ggc 2412
Asn Leu His Lys Thr Phe Thr Ile Pro His Gly Gly Gly Gly Pro Gly
690 695 700
gtg ggc ccg gtg tgc gtc gcg gag cac ctc gtc ccc ttc ctg ccg gcg 2460
Val Gly Pro Val Cys Val Ala Glu His Leu Val Pro Phe Leu Pro Ala
705 710 715 720
gac ccg acc tcc ctc gac ggc gcc acc ccg gcg gcg gtc ggc acc ggc 2508
Asp Pro Thr Ser Leu Asp Gly Ala Thr Pro Ala Ala Val Gly Thr Gly
725 730 735
gtg ccc gtc tct gcg acg cgc cac ggc tcg gcg ggc gtc ctg cag atc 2556
Val Pro Val Ser Ala Thr Arg His Gly Ser Ala Gly Val Leu Gln Ile
740 745 750
tcc tgg gcg tac atc gcc atg atg ggc acc gac ggc ctc acc cgc gcc 2604
Ser Trp Ala Tyr Ile Ala Met Met Gly Thr Asp Gly Leu Thr Arg Ala
755 760 765
agc gcc gcg gcg atc ctc agc gcg aac tac gtc gcc cac cgc ctg ggc 2652
Ser Ala Ala Ala Ile Leu Ser Ala Asn Tyr Val Ala His Arg Leu Gly
770 775 780
gag gcc ttc ccg gtg ctc tac acc ggc cgc aac ggc ctg gtg gcg cac 2700
Glu Ala Phe Pro Val Leu Tyr Thr Gly Arg Asn Gly Leu Val Ala His
785 790 795 800
gag tgc atc ctc gac ctg cgc gac ctg acg agg cag acc ggc gtc acc 2748
Glu Cys Ile Leu Asp Leu Arg Asp Leu Thr Arg Gln Thr Gly Val Thr
805 810 815
gcc acg gac gtg gcc aaa cgc ctc atc gac ttc ggc ttc cac gcc ccc 2796
Ala Thr Asp Val Ala Lys Arg Leu Ile Asp Phe Gly Phe His Ala Pro
820 825 830
acg ctc tcc ttc ccc gtc gcc ggt acc ctc atg gtc gag ccg acc gag 2844
Thr Leu Ser Phe Pro Val Ala Gly Thr Leu Met Val Glu Pro Thr Glu
835 840 845
tcc gag gac ctc gcc gag ctc gac cgc ttc atc gag gcg atg ctc acc 2892
Ser Glu Asp Leu Ala Glu Leu Asp Arg Phe Ile Glu Ala Met Leu Thr
850 855 860
atc cgc gcg gag atc gac gag atc agc ggc ggc gac atc gcc tac gag 2940
Ile Arg Ala Glu Ile Asp Glu Ile Ser Gly Gly Asp Ile Ala Tyr Glu
865 870 875 880
gac tcc gtc ctc cac cac gcg ccg ttc acc gcg tgg agc gtg tcc cgc 2988
Asp Ser Val Leu His His Ala Pro Phe Thr Ala Trp Ser Val Ser Arg
885 890 895
gac gac tgg gac cac gcc ttc acc cgt gag cag gcg gcc tgg ccg gtc 3036
Asp Asp Trp Asp His Ala Phe Thr Arg Glu Gln Ala Ala Trp Pro Val
900 905 910
ccc ggt ctg cgc cgc acc aag tac ttc ccg ccg gtc cgc cgc ctc aac 3084
Pro Gly Leu Arg Arg Thr Lys Tyr Phe Pro Pro Val Arg Arg Leu Asn
915 920 925
gag gcc tac ggt gac cgc cac ctc gtg tgc gcc tgc ccg ccg ccc gag 3132
Glu Ala Tyr Gly Asp Arg His Leu Val Cys Ala Cys Pro Pro Pro Glu
930 935 940
gcc ttc gac ctt tcc gag gag aac cag tga ccgacctgcg taccagcccc 3182
Ala Phe Asp Leu Ser Glu Glu Asn Gln
945 950
ctccacgagc gtcatgtgga gctcggggcc tccttcaccc ccttcggccc gtggaacatg 3242
cccctgcgct acggcaacga gctcgacgag caccgcgccg tccgttccac cgccgggctc 3302
ttcgacctct cgcacatggg tgagatccgc gtgtggggcg cacaggccgc ggagttcctc 3362
gaccatgcgc tgctctccgc cttctccacg ctccccgtgg gtaaggcgcg gtactccatg 3422
cttgtcgacg ccaccggtgc catcctcgac gacctcctca 3462
<210> 40
<211> 953
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 40
Met Ala Leu His Pro Ile Thr Asp Ser Ala Ser Tyr Leu Ala Arg His
1 5 10 15
Leu Gly Pro Asp Thr Ala Glu Glu Gln Val Met Leu Gly Ala Leu Gly
20 25 30
Tyr Gly Ser Leu Ala Glu Leu Val Asp Ala Ala Leu Pro Thr Asp Ile
35 40 45
Arg Leu Thr Glu Pro Leu Pro Arg His Ala Pro Leu Ser Glu Thr Glu
50 55 60
Ala Gln Ala Arg Leu Arg Glu Tyr Ala Ser Arg Asn Thr Val Leu Arg
65 70 75 80
Ser Phe Tyr Gly Gln Gly Phe Ser Asp Thr Val Thr Pro Pro Val Ile
85 90 95
Arg Arg Gly Val Leu Glu Ala Pro Gly Trp Tyr Thr Ala Tyr Thr Pro
100 105 110
Tyr Gln Pro Glu Ile Ser Gln Gly Arg Leu Glu Ala Leu Leu Asn Phe
115 120 125
Gln Thr Met Val Ser Glu Leu Thr Gly Leu Pro Val Ala Asn Ala Ser
130 135 140
Leu Leu Asp Glu Ala Ser Ala Thr Ala Glu Ala Val Gly Leu Met Ser
145 150 155 160
Arg Ala Gln Arg Lys Gly Arg Arg Val Leu Leu Asp Ala Arg Leu His
165 170 175
Pro Gln Val Leu Ala Val Ala Ala Glu Arg Ala Arg Ala Ile Asp Leu
180 185 190
Glu Val Ala Val Glu Asp Leu Ser Gly Pro Leu Val Gly Glu Asp Leu
195 200 205
Cys Gly Val Val Ile Ala Tyr Pro Gly Thr Glu Gly Asp Ile Ala Asp
210 215 220
Pro Arg Pro Val Ile Glu Ala Val His Ala Arg Gly Gly Leu Ala Thr
225 230 235 240
Val Val Thr Asp Pro Leu Ser Leu Leu Leu Leu Glu Ser Pro Gly Glu
245 250 255
Met Gly Ala Asp Ile Ala Val Gly Ser Ser Gln Arg Phe Gly Val Pro
260 265 270
Leu Phe Tyr Gly Gly Pro His Ala Ala Tyr Met Ala Val Ser Asp Ala
275 280 285
Leu Lys Arg Gln Ile Pro Gly Arg Leu Val Gly Val Ser Val Asp Ala
290 295 300
Glu Gly Ala Pro Ala Tyr Arg Leu Ala Leu Gln Thr Arg Glu Gln His
305 310 315 320
Ile Arg Arg Glu Arg Ala Thr Ser Asn Ile Cys Thr Ala Gln Ala Leu
325 330 335
Leu Ala Val Thr Ala Gly Met Tyr Ala Val Tyr His Gly Pro Glu Gly
340 345 350
Leu Arg Ala Ile Ala Glu Arg Val His Gly Leu Ala Cys Asp Phe Ala
355 360 365
Ala Ala Val Arg Ala Ala Gly Gly Thr Val Leu His Asp Ala Phe Phe
370 375 380
Asp Thr Val Ala Val Arg Val Glu Ala Ala Arg Pro Ile Val Gly Thr
385 390 395 400
Leu Ala Glu Ala Gly Tyr Leu Val Arg Ala Val Asp Gly Thr Thr Val
405 410 415
Gly Val Ser Phe Gly Glu Ser Ala Glu Glu Gly Asp Val Glu Lys Leu
420 425 430
Ala Ala Val Phe Gly Ala Ser Leu Thr Ala Gly Thr Arg Cys Ile Pro
435 440 445
Ala Ser Val Ala Arg Gln Thr Pro Thr Leu Thr His Pro Val Phe Ser
450 455 460
Thr Leu His Ser Glu Thr Gln Met Met Arg Tyr Leu Arg Thr Leu Ser
465 470 475 480
Asp Arg Asp Leu Ala Leu Asp Arg Thr Met Ile Pro Leu Gly Ser Cys
485 490 495
Thr Met Lys Leu Asn Pro Thr Ala Gly Leu Glu Thr Ile Thr Trp Pro
500 505 510
Glu Phe Ala Asp Met His Pro Phe Ala Pro Asp Asp Gln Ala Ala Gly
515 520 525
Trp Leu Ala Leu Ile Ala Glu Leu Glu Gly Trp Leu Ala Glu Leu Thr
530 535 540
Gly Tyr Ala Lys Val Ser Val Gln Pro Asn Ala Gly Ser Gln Gly Glu
545 550 555 560
Leu Ala Gly Leu Leu Ala Ile Arg Arg Tyr His Val Ala Asn Gly Asp
565 570 575
Met Asp Arg Asp Ile Cys Leu Val Pro Ala Ser Ala His Gly Thr Asn
580 585 590
Ala Ala Ser Ala Thr Leu Ala His Leu Arg Val Val Val Val Gly Thr
595 600 605
Ala Gly Asp Gly Ser Ile Asp Met Asp Asp Leu Asp Ala Lys Leu Ala
610 615 620
Glu His Gly Pro Gln Val Ala Ala Ile Met Ile Thr Tyr Pro Ser Thr
625 630 635 640
His Gly Val Phe Glu Asp Thr Val Arg Glu Val Cys Asp Arg Val His
645 650 655
Ala Val Gly Gly Gln Val Tyr Ile Asp Gly Ala Asn Leu Asn Ala Leu
660 665 670
Thr Gly Leu Ala Leu Leu Gly Arg Ile Gly Gly Asp Val Ser His Leu
675 680 685
Asn Leu His Lys Thr Phe Thr Ile Pro His Gly Gly Gly Gly Pro Gly
690 695 700
Val Gly Pro Val Cys Val Ala Glu His Leu Val Pro Phe Leu Pro Ala
705 710 715 720
Asp Pro Thr Ser Leu Asp Gly Ala Thr Pro Ala Ala Val Gly Thr Gly
725 730 735
Val Pro Val Ser Ala Thr Arg His Gly Ser Ala Gly Val Leu Gln Ile
740 745 750
Ser Trp Ala Tyr Ile Ala Met Met Gly Thr Asp Gly Leu Thr Arg Ala
755 760 765
Ser Ala Ala Ala Ile Leu Ser Ala Asn Tyr Val Ala His Arg Leu Gly
770 775 780
Glu Ala Phe Pro Val Leu Tyr Thr Gly Arg Asn Gly Leu Val Ala His
785 790 795 800
Glu Cys Ile Leu Asp Leu Arg Asp Leu Thr Arg Gln Thr Gly Val Thr
805 810 815
Ala Thr Asp Val Ala Lys Arg Leu Ile Asp Phe Gly Phe His Ala Pro
820 825 830
Thr Leu Ser Phe Pro Val Ala Gly Thr Leu Met Val Glu Pro Thr Glu
835 840 845
Ser Glu Asp Leu Ala Glu Leu Asp Arg Phe Ile Glu Ala Met Leu Thr
850 855 860
Ile Arg Ala Glu Ile Asp Glu Ile Ser Gly Gly Asp Ile Ala Tyr Glu
865 870 875 880
Asp Ser Val Leu His His Ala Pro Phe Thr Ala Trp Ser Val Ser Arg
885 890 895
Asp Asp Trp Asp His Ala Phe Thr Arg Glu Gln Ala Ala Trp Pro Val
900 905 910
Pro Gly Leu Arg Arg Thr Lys Tyr Phe Pro Pro Val Arg Arg Leu Asn
915 920 925
Glu Ala Tyr Gly Asp Arg His Leu Val Cys Ala Cys Pro Pro Pro Glu
930 935 940
Ala Phe Asp Leu Ser Glu Glu Asn Gln
945 950
<210> 41
<211> 1704
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1404)
<223> ген gcvT
<400> 41
cgagctcgac cgcttcatcg aggcgatgct caccatccgc gcggagatcg acgagatcag 60
cggcggcgac atcgcctacg aggactccgt cctccaccac gcgccgttca ccgcgtggag 120
cgtgtcccgc gacgactggg accacgcctt cacccgtgag caggcggcct ggccggtccc 180
cggtctgcgc cgcaccaagt acttcccgcc ggtccgccgc ctcaacgagg cctacggtga 240
ccgccacctc gtgtgcgcct gcccgccgcc cgaggccttc gacctttccg aggagaacca 300
gtg acc gac ctg cgt acc agc ccc ctc cac gag cgt cat gtg gag ctc 348
Val Thr Asp Leu Arg Thr Ser Pro Leu His Glu Arg His Val Glu Leu
1 5 10 15
ggg gcc tcc ttc acc ccc ttc ggc ccg tgg aac atg ccc ctg cgc tac 396
Gly Ala Ser Phe Thr Pro Phe Gly Pro Trp Asn Met Pro Leu Arg Tyr
20 25 30
ggc aac gag ctc gac gag cac cgc gcc gtc cgt tcc acc gcc ggg ctc 444
Gly Asn Glu Leu Asp Glu His Arg Ala Val Arg Ser Thr Ala Gly Leu
35 40 45
ttc gac ctc tcg cac atg ggt gag atc cgc gtg tgg ggc gca cag gcc 492
Phe Asp Leu Ser His Met Gly Glu Ile Arg Val Trp Gly Ala Gln Ala
50 55 60
gcg gag ttc ctc gac cat gcg ctg ctc tcc gcc ttc tcc acg ctc ccc 540
Ala Glu Phe Leu Asp His Ala Leu Leu Ser Ala Phe Ser Thr Leu Pro
65 70 75 80
gtg ggt aag gcg cgg tac tcc atg ctt gtc gac gcc acc ggt gcc atc 588
Val Gly Lys Ala Arg Tyr Ser Met Leu Val Asp Ala Thr Gly Ala Ile
85 90 95
ctc gac gac ctc ctc acc tac cgc ctc gcc ggg gac gag ttc ctg gtg 636
Leu Asp Asp Leu Leu Thr Tyr Arg Leu Ala Gly Asp Glu Phe Leu Val
100 105 110
gtc ccc aac gcg gcg aac acg gag acc gtg tgg gag gag ttc cgg gcc 684
Val Pro Asn Ala Ala Asn Thr Glu Thr Val Trp Glu Glu Phe Arg Ala
115 120 125
cgc gcc gcc ggc ttc gac gtg gag ctg gcc gac gag tcc gcc gac act 732
Arg Ala Ala Gly Phe Asp Val Glu Leu Ala Asp Glu Ser Ala Asp Thr
130 135 140
gcg ctg ctg gct gtg cag ggc ccc cgc gcc gag cag atc atc gcc gac 780
Ala Leu Leu Ala Val Gln Gly Pro Arg Ala Glu Gln Ile Ile Ala Asp
145 150 155 160
ctc gtc gcc ggg gac gac gag tcg gtc gtg cgc acg atg aag ttc tac 828
Leu Val Ala Gly Asp Asp Glu Ser Val Val Arg Thr Met Lys Phe Tyr
165 170 175
tcg gcg gcc ccg ttg acg gtg gcg ggc gtc gat acg ctg ctc gcc cgc 876
Ser Ala Ala Pro Leu Thr Val Ala Gly Val Asp Thr Leu Leu Ala Arg
180 185 190
acc ggc tac acc ggg gag gac ggc ttc gag ctc tac gtc tcc ggc gcg 924
Thr Gly Tyr Thr Gly Glu Asp Gly Phe Glu Leu Tyr Val Ser Gly Ala
195 200 205
gac gcc gct gaa ctg tgg gac gcg ctg ctc aac gcc ggc gcc gcc tac 972
Asp Ala Ala Glu Leu Trp Asp Ala Leu Leu Asn Ala Gly Ala Ala Tyr
210 215 220
gac atg ctg ccc gcc gga ctg gcg gcg cgg gac tcc ctg cgc ctg gag 1020
Asp Met Leu Pro Ala Gly Leu Ala Ala Arg Asp Ser Leu Arg Leu Glu
225 230 235 240
gcc ggg ctc ccc ctc tac ggg cag gag ctc acc cgc gac atc acg ccg 1068
Ala Gly Leu Pro Leu Tyr Gly Gln Glu Leu Thr Arg Asp Ile Thr Pro
245 250 255
gtg gag gcc ggg atg gcc cgc gcc ttc gcc tcg aag gag ggg gac ttc 1116
Val Glu Ala Gly Met Ala Arg Ala Phe Ala Ser Lys Glu Gly Asp Phe
260 265 270
gtc ggc cgc gac gcc ctc gtc ggc agg gag ccg acc agc gcc atc gtc 1164
Val Gly Arg Asp Ala Leu Val Gly Arg Glu Pro Thr Ser Ala Ile Val
275 280 285
ggg ctc cgg ggt ctg ggg cgc cgc gcg gcc cgc gag ggc gcc gag gtc 1212
Gly Leu Arg Gly Leu Gly Arg Arg Ala Ala Arg Glu Gly Ala Glu Val
290 295 300
tac ctc gac ggc gaa cag gtc ggc gtg gtc acc tcc ggg cag ccg gcg 1260
Tyr Leu Asp Gly Glu Gln Val Gly Val Val Thr Ser Gly Gln Pro Ala
305 310 315 320
ccg gtg ctc ggc tac ccg atc gca ctc gcc cgc ctg cgc ccc gac gtc 1308
Pro Val Leu Gly Tyr Pro Ile Ala Leu Ala Arg Leu Arg Pro Asp Val
325 330 335
acc gcg gcc ggc acg gca ctg gag gtg gac atc cgt ggg agg cgg cac 1356
Thr Ala Ala Gly Thr Ala Leu Glu Val Asp Ile Arg Gly Arg Arg His
340 345 350
ccc ttc gag atc gtc gac ctg ccc ttc tac cgc cgc gcc gca cga taa 1404
Pro Phe Glu Ile Val Asp Leu Pro Phe Tyr Arg Arg Ala Ala Arg
355 360 365
cctgaaccca ccaccaacgg aaaggctccc ccaccatggc caccgagcac tccctgcccg 1464
ggaactactc ctactccgag gaccacgagt ggatcgacgc gacgcccgag gacgccgtcg 1524
gcgcgaccgt gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct 1584
acgcggagct cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg 1644
agtccacgaa gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca 1704
<210> 42
<211> 367
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 42
Val Thr Asp Leu Arg Thr Ser Pro Leu His Glu Arg His Val Glu Leu
1 5 10 15
Gly Ala Ser Phe Thr Pro Phe Gly Pro Trp Asn Met Pro Leu Arg Tyr
20 25 30
Gly Asn Glu Leu Asp Glu His Arg Ala Val Arg Ser Thr Ala Gly Leu
35 40 45
Phe Asp Leu Ser His Met Gly Glu Ile Arg Val Trp Gly Ala Gln Ala
50 55 60
Ala Glu Phe Leu Asp His Ala Leu Leu Ser Ala Phe Ser Thr Leu Pro
65 70 75 80
Val Gly Lys Ala Arg Tyr Ser Met Leu Val Asp Ala Thr Gly Ala Ile
85 90 95
Leu Asp Asp Leu Leu Thr Tyr Arg Leu Ala Gly Asp Glu Phe Leu Val
100 105 110
Val Pro Asn Ala Ala Asn Thr Glu Thr Val Trp Glu Glu Phe Arg Ala
115 120 125
Arg Ala Ala Gly Phe Asp Val Glu Leu Ala Asp Glu Ser Ala Asp Thr
130 135 140
Ala Leu Leu Ala Val Gln Gly Pro Arg Ala Glu Gln Ile Ile Ala Asp
145 150 155 160
Leu Val Ala Gly Asp Asp Glu Ser Val Val Arg Thr Met Lys Phe Tyr
165 170 175
Ser Ala Ala Pro Leu Thr Val Ala Gly Val Asp Thr Leu Leu Ala Arg
180 185 190
Thr Gly Tyr Thr Gly Glu Asp Gly Phe Glu Leu Tyr Val Ser Gly Ala
195 200 205
Asp Ala Ala Glu Leu Trp Asp Ala Leu Leu Asn Ala Gly Ala Ala Tyr
210 215 220
Asp Met Leu Pro Ala Gly Leu Ala Ala Arg Asp Ser Leu Arg Leu Glu
225 230 235 240
Ala Gly Leu Pro Leu Tyr Gly Gln Glu Leu Thr Arg Asp Ile Thr Pro
245 250 255
Val Glu Ala Gly Met Ala Arg Ala Phe Ala Ser Lys Glu Gly Asp Phe
260 265 270
Val Gly Arg Asp Ala Leu Val Gly Arg Glu Pro Thr Ser Ala Ile Val
275 280 285
Gly Leu Arg Gly Leu Gly Arg Arg Ala Ala Arg Glu Gly Ala Glu Val
290 295 300
Tyr Leu Asp Gly Glu Gln Val Gly Val Val Thr Ser Gly Gln Pro Ala
305 310 315 320
Pro Val Leu Gly Tyr Pro Ile Ala Leu Ala Arg Leu Arg Pro Asp Val
325 330 335
Thr Ala Ala Gly Thr Ala Leu Glu Val Asp Ile Arg Gly Arg Arg His
340 345 350
Pro Phe Glu Ile Val Asp Leu Pro Phe Tyr Arg Arg Ala Ala Arg
355 360 365
<210> 43
<211> 1899
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1599)
<223> ген glyA
<400> 43
gacaggggga gatagacctc ggcgacctcc acgaggtcga tcgattcgcc gatgccgcgg 60
agctgctcga cctcctcctc cgtgagcacc tgcggcatcg attgacgcag ggcacgccag 120
gaggagcggt cgaaatccac gtagggggtg gcgtccttgg gccgagacat gcgtcccatt 180
gtgtcatctg ccccgtcaac tgtgtcccac tcacccgggg tgcgggggta tgaggtgccc 240
tgtgggcggt atgctgtcca cgaatgccgt gtacccctag aaaggctggc tgtaacgccc 300
atg acc gct gag tct tct gat ctc cgt tac cag gaa ctg tcc gtc ctc 348
Met Thr Ala Glu Ser Ser Asp Leu Arg Tyr Gln Glu Leu Ser Val Leu
1 5 10 15
gac ccg gag gtc cac gcc gcc atc ggc ggt gag atc gcc cgt cag cgt 396
Asp Pro Glu Val His Ala Ala Ile Gly Gly Glu Ile Ala Arg Gln Arg
20 25 30
gac ttc ctc gag atg atc gcg tcc gag aac ttc gtc ccc cgt gcc gtc 444
Asp Phe Leu Glu Met Ile Ala Ser Glu Asn Phe Val Pro Arg Ala Val
35 40 45
ctg cag gca cag ggt tcg gtg ttc acc aac aag tac gcc gag ggc tac 492
Leu Gln Ala Gln Gly Ser Val Phe Thr Asn Lys Tyr Ala Glu Gly Tyr
50 55 60
ccg ggg cgt cgt tac tac ggt ggc tgc gag aac gcc gac atc gtc gag 540
Pro Gly Arg Arg Tyr Tyr Gly Gly Cys Glu Asn Ala Asp Ile Val Glu
65 70 75 80
gac ctg gcc cgc gac cgc gcc aag gcc ctg ttc ggc gcc gag ttc gcc 588
Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe Gly Ala Glu Phe Ala
85 90 95
aac gtc cag ccg cac tcc ggc gcc cag gcc aac gcc gcc gtc ctg cac 636
Asn Val Gln Pro His Ser Gly Ala Gln Ala Asn Ala Ala Val Leu His
100 105 110
gcg ctg gcc gac gcc ggc gac aag atc atg ggt ctg tcc ctg gcc cac 684
Ala Leu Ala Asp Ala Gly Asp Lys Ile Met Gly Leu Ser Leu Ala His
115 120 125
ggt ggt cac ctg acc cac ggc atg aag ctc aac ttc tcc ggc aag ctc 732
Gly Gly His Leu Thr His Gly Met Lys Leu Asn Phe Ser Gly Lys Leu
130 135 140
tac gac gtc gtc gcc tac gag gtc gac ccg gag acc cac cgc atc gac 780
Tyr Asp Val Val Ala Tyr Glu Val Asp Pro Glu Thr His Arg Ile Asp
145 150 155 160
atg gac cgc gtc cgc gag cag gcc ctg gcc gag cgc ccg aag gtc ctc 828
Met Asp Arg Val Arg Glu Gln Ala Leu Ala Glu Arg Pro Lys Val Leu
165 170 175
atc gcc ggc tgg tcc gcg tac ccg cgc acc ctc gac ttc gag gcg ttc 876
Ile Ala Gly Trp Ser Ala Tyr Pro Arg Thr Leu Asp Phe Glu Ala Phe
180 185 190
cgc tcc atc gcc gac gag gtc ggc gcg tac ctg tgg acc gac atg gcg 924
Arg Ser Ile Ala Asp Glu Val Gly Ala Tyr Leu Trp Thr Asp Met Ala
195 200 205
cac ttc gcc ggc ctc gtc gcc gcc gac ctg cac ccg tcc ccg gtc ccg 972
His Phe Ala Gly Leu Val Ala Ala Asp Leu His Pro Ser Pro Val Pro
210 215 220
cac tcc gac gtc gtc tcc acc acc gtc cac aag acg ctg ggt ggc ccg 1020
His Ser Asp Val Val Ser Thr Thr Val His Lys Thr Leu Gly Gly Pro
225 230 235 240
cgt tcc ggc ctg atc gtc gcg aag cag gag tgg gcc aag aag ctc aac 1068
Arg Ser Gly Leu Ile Val Ala Lys Gln Glu Trp Ala Lys Lys Leu Asn
245 250 255
tcc gcc gtc ttc ccg ggc cag cag ggt ggt ccg ctc atg cac gcc atc 1116
Ser Ala Val Phe Pro Gly Gln Gln Gly Gly Pro Leu Met His Ala Ile
260 265 270
gcc gcc aag gcc gtg gca ctg aag gtc gcc ggc acc gac gag ttc aag 1164
Ala Ala Lys Ala Val Ala Leu Lys Val Ala Gly Thr Asp Glu Phe Lys
275 280 285
gac cgt cag gcc cgc acc atc gag ggt gcc aag atc ctg gcc gag cgt 1212
Asp Arg Gln Ala Arg Thr Ile Glu Gly Ala Lys Ile Leu Ala Glu Arg
290 295 300
ctc acc gcc gct gac gcg aag gcc gcc ggc atc gac gtc ctc acc ggt 1260
Leu Thr Ala Ala Asp Ala Lys Ala Ala Gly Ile Asp Val Leu Thr Gly
305 310 315 320
ggc acc gac gtg cac ctg gtc ctc gct gac ctg cgc aac tcc gag atg 1308
Gly Thr Asp Val His Leu Val Leu Ala Asp Leu Arg Asn Ser Glu Met
325 330 335
gac ggc cag cag gcc gaa gac ctc ctc cac gag gtc ggc atc acc gtc 1356
Asp Gly Gln Gln Ala Glu Asp Leu Leu His Glu Val Gly Ile Thr Val
340 345 350
aac cgc aac gcc gtc ccg aac gac ccg cgc ccg ccg atg gtc acc tcc 1404
Asn Arg Asn Ala Val Pro Asn Asp Pro Arg Pro Pro Met Val Thr Ser
355 360 365
ggc ctg cgc atc ggc acc ccg gcc ctg gcc acc cgt ggt ttc gac gcc 1452
Gly Leu Arg Ile Gly Thr Pro Ala Leu Ala Thr Arg Gly Phe Asp Ala
370 375 380
gcc gcc ttc acc gag gtc gcc gac atc atc ggc acc gcc ctg gtc aac 1500
Ala Ala Phe Thr Glu Val Ala Asp Ile Ile Gly Thr Ala Leu Val Asn
385 390 395 400
ggc aag aac gcc gac atc gag gca ctg cgt gcc cgc gtc gcg aag ctg 1548
Gly Lys Asn Ala Asp Ile Glu Ala Leu Arg Ala Arg Val Ala Lys Leu
405 410 415
acc gac cag tac ccg ctg tac gag ggt ctg gag gag tgg aag atc aac 1596
Thr Asp Gln Tyr Pro Leu Tyr Glu Gly Leu Glu Glu Trp Lys Ile Asn
420 425 430
tag gtcttcccgc cctccccgtg cgcgtcctcg tcatagacaa cctcgattcc 1649
ttcacctgga atctcgtcgc ctacgtcgag gacgtcaccg gtgtcgcccc cgaggtgatc 1709
accaacaacg aacccggatg ggatgtcgac cgcgtcgcca cctacgacgc cgtcatcatc 1769
tccccgggcc ccggccgccc ggagcgtgag aaggacatcg gcatgtgcct cgatgtcctg 1829
cgcgacgggc gtgtcccgat tctgggggtc tgcctcgggc accaggccct cgcccacgcg 1889
tggggcggca 1899
<210> 44
<211> 432
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 44
Met Thr Ala Glu Ser Ser Asp Leu Arg Tyr Gln Glu Leu Ser Val Leu
1 5 10 15
Asp Pro Glu Val His Ala Ala Ile Gly Gly Glu Ile Ala Arg Gln Arg
20 25 30
Asp Phe Leu Glu Met Ile Ala Ser Glu Asn Phe Val Pro Arg Ala Val
35 40 45
Leu Gln Ala Gln Gly Ser Val Phe Thr Asn Lys Tyr Ala Glu Gly Tyr
50 55 60
Pro Gly Arg Arg Tyr Tyr Gly Gly Cys Glu Asn Ala Asp Ile Val Glu
65 70 75 80
Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe Gly Ala Glu Phe Ala
85 90 95
Asn Val Gln Pro His Ser Gly Ala Gln Ala Asn Ala Ala Val Leu His
100 105 110
Ala Leu Ala Asp Ala Gly Asp Lys Ile Met Gly Leu Ser Leu Ala His
115 120 125
Gly Gly His Leu Thr His Gly Met Lys Leu Asn Phe Ser Gly Lys Leu
130 135 140
Tyr Asp Val Val Ala Tyr Glu Val Asp Pro Glu Thr His Arg Ile Asp
145 150 155 160
Met Asp Arg Val Arg Glu Gln Ala Leu Ala Glu Arg Pro Lys Val Leu
165 170 175
Ile Ala Gly Trp Ser Ala Tyr Pro Arg Thr Leu Asp Phe Glu Ala Phe
180 185 190
Arg Ser Ile Ala Asp Glu Val Gly Ala Tyr Leu Trp Thr Asp Met Ala
195 200 205
His Phe Ala Gly Leu Val Ala Ala Asp Leu His Pro Ser Pro Val Pro
210 215 220
His Ser Asp Val Val Ser Thr Thr Val His Lys Thr Leu Gly Gly Pro
225 230 235 240
Arg Ser Gly Leu Ile Val Ala Lys Gln Glu Trp Ala Lys Lys Leu Asn
245 250 255
Ser Ala Val Phe Pro Gly Gln Gln Gly Gly Pro Leu Met His Ala Ile
260 265 270
Ala Ala Lys Ala Val Ala Leu Lys Val Ala Gly Thr Asp Glu Phe Lys
275 280 285
Asp Arg Gln Ala Arg Thr Ile Glu Gly Ala Lys Ile Leu Ala Glu Arg
290 295 300
Leu Thr Ala Ala Asp Ala Lys Ala Ala Gly Ile Asp Val Leu Thr Gly
305 310 315 320
Gly Thr Asp Val His Leu Val Leu Ala Asp Leu Arg Asn Ser Glu Met
325 330 335
Asp Gly Gln Gln Ala Glu Asp Leu Leu His Glu Val Gly Ile Thr Val
340 345 350
Asn Arg Asn Ala Val Pro Asn Asp Pro Arg Pro Pro Met Val Thr Ser
355 360 365
Gly Leu Arg Ile Gly Thr Pro Ala Leu Ala Thr Arg Gly Phe Asp Ala
370 375 380
Ala Ala Phe Thr Glu Val Ala Asp Ile Ile Gly Thr Ala Leu Val Asn
385 390 395 400
Gly Lys Asn Ala Asp Ile Glu Ala Leu Arg Ala Arg Val Ala Lys Leu
405 410 415
Thr Asp Gln Tyr Pro Leu Tyr Glu Gly Leu Glu Glu Trp Lys Ile Asn
420 425 430
<210> 45
<211> 1932
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1632)
<223> ген hom
<400> 45
gcggcgtgcg cggacgagat ccccgccagt gggatcacca ggcacgccac gcaggccgtg 60
agtgcacggg tgatccgacg gcgggacatg ctgactcctg tcactggcag aggcatgggg 120
gtggtgagga cagattaatc gtctgaacgc aatatgtcca gagatgtgac ttatcttatg 180
gagtgtcgtg accccggagt ctgaggaagt ggccgaatcc cggtagggtg gtcacggaaa 240
tctgtcctca gtctgcccgt ccccgacggg cacggaccca cgaccatgtg caggagaacc 300
atg acc gtc act cag cag ccc agc ttc aac ccc ggc aag gga gcc ggc 348
Met Thr Val Thr Gln Gln Pro Ser Phe Asn Pro Gly Lys Gly Ala Gly
1 5 10 15
gag ccg gtg ggc gtc gcc att ctc ggc cac ggc acc gtc gga tct gag 396
Glu Pro Val Gly Val Ala Ile Leu Gly His Gly Thr Val Gly Ser Glu
20 25 30
gtg ctg cgc ctg atg ggc gag tac tcc gac gcg ctc gcg cac cgc atc 444
Val Leu Arg Leu Met Gly Glu Tyr Ser Asp Ala Leu Ala His Arg Ile
35 40 45
ggc ggt ccg ctc gag ctc aag ggt gtg gcc gtg tcc gac ctc tcc aag 492
Gly Gly Pro Leu Glu Leu Lys Gly Val Ala Val Ser Asp Leu Ser Lys
50 55 60
ccg cgc gag ggc gtg gac cgt gag ctg ctc acc gat gac gcg ctg agc 540
Pro Arg Glu Gly Val Asp Arg Glu Leu Leu Thr Asp Asp Ala Leu Ser
65 70 75 80
ctc atc aac cgc gac gac gtc gac atc gtc gtc gag gtc atc ggc ggc 588
Leu Ile Asn Arg Asp Asp Val Asp Ile Val Val Glu Val Ile Gly Gly
85 90 95
atc gag tac ccg cgt gag ctg gtc ctg gcc gcc ctg cgc gcc ggc aag 636
Ile Glu Tyr Pro Arg Glu Leu Val Leu Ala Ala Leu Arg Ala Gly Lys
100 105 110
tcc gtc gtc acc gcc aac aag gcc ctc gtc gcc gcg cac gcc gac gag 684
Ser Val Val Thr Ala Asn Lys Ala Leu Val Ala Ala His Ala Asp Glu
115 120 125
ctt gcc gac gcc gcc gac gcc gcc aac gtc gac ctc tac ttc gag gcc 732
Leu Ala Asp Ala Ala Asp Ala Ala Asn Val Asp Leu Tyr Phe Glu Ala
130 135 140
gcc gtc gcc gcc gcc atc ccg gtc gtc ggc atg ctg cgt cgt tcc ctc 780
Ala Val Ala Ala Ala Ile Pro Val Val Gly Met Leu Arg Arg Ser Leu
145 150 155 160
gcc ggc gac aac atc ctg cgt atc tcc ggc atc gtc aac ggc acc acc 828
Ala Gly Asp Asn Ile Leu Arg Ile Ser Gly Ile Val Asn Gly Thr Thr
165 170 175
aac ttc atc ctc gac gcg atg gag tcc acc ggc gcc agc tac gac gac 876
Asn Phe Ile Leu Asp Ala Met Glu Ser Thr Gly Ala Ser Tyr Asp Asp
180 185 190
gcg ctc gcc gag gcc acc cgt ctg ggc tac gcc gag gcc gac ccg acc 924
Ala Leu Ala Glu Ala Thr Arg Leu Gly Tyr Ala Glu Ala Asp Pro Thr
195 200 205
gcc gac gtc gag ggc cac gac gcc gcc tcc aag gcc gcc atc ctc gcc 972
Ala Asp Val Glu Gly His Asp Ala Ala Ser Lys Ala Ala Ile Leu Ala
210 215 220
tcc ctg ggc ttc cac acc cgc gtc aag ttc gac gac gtc agc tgc gag 1020
Ser Leu Gly Phe His Thr Arg Val Lys Phe Asp Asp Val Ser Cys Glu
225 230 235 240
ggc atc tcc aag gtc acc gcc gag gac atc gag gcc gcc cgc gac gcc 1068
Gly Ile Ser Lys Val Thr Ala Glu Asp Ile Glu Ala Ala Arg Asp Ala
245 250 255
ggc tac gtg atc aag ctg ctc gcc atc tgt gag cgc gtc acc gac aag 1116
Gly Tyr Val Ile Lys Leu Leu Ala Ile Cys Glu Arg Val Thr Asp Lys
260 265 270
gac ggc gtg gag tcc gtc gcc agc cgc gtc cac ccg acc ctc gtt ccg 1164
Asp Gly Val Glu Ser Val Ala Ser Arg Val His Pro Thr Leu Val Pro
275 280 285
gtc gac cac ccg ctg gcc tcg gtc aac cag tcc tac aac gcc atc ttc 1212
Val Asp His Pro Leu Ala Ser Val Asn Gln Ser Tyr Asn Ala Ile Phe
290 295 300
gtc gag gcc gag gca gcc ggt cgc ctg atg ttc tac ggc aac ggt gcc 1260
Val Glu Ala Glu Ala Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala
305 310 315 320
ggt ggc gcc ccg acc gcc tcc gcc gtc ctc ggt gac ctc gtc ggc gcc 1308
Gly Gly Ala Pro Thr Ala Ser Ala Val Leu Gly Asp Leu Val Gly Ala
325 330 335
gcc cgc aac aag gtc cac ggt ggc cgc gcc ccg ggc gag aac acc tac 1356
Ala Arg Asn Lys Val His Gly Gly Arg Ala Pro Gly Glu Asn Thr Tyr
340 345 350
gcg aac ctg ccg atc gcc gac ctc ggc gac gtg cgc acc cgc tac cac 1404
Ala Asn Leu Pro Ile Ala Asp Leu Gly Asp Val Arg Thr Arg Tyr His
355 360 365
atc gac atg gag gtc gag gac cgc acc ggt gtc ctc gcc gag ctg tcc 1452
Ile Asp Met Glu Val Glu Asp Arg Thr Gly Val Leu Ala Glu Leu Ser
370 375 380
gcg atc ttc gcc acc cac gac atc tcc ctc aag acc gtc cgc cag gag 1500
Ala Ile Phe Ala Thr His Asp Ile Ser Leu Lys Thr Val Arg Gln Glu
385 390 395 400
gag gcc ggc gac cac gcc cgc ctc atc gtc gtc acg cat tcc gca cgt 1548
Glu Ala Gly Asp His Ala Arg Leu Ile Val Val Thr His Ser Ala Arg
405 410 415
gag gcc gac ctg gcc gcc acc gtc gag gac ctg aag aag gcc ccg gcg 1596
Glu Ala Asp Leu Ala Ala Thr Val Glu Asp Leu Lys Lys Ala Pro Ala
420 425 430
gtc aag tcc gtc gac agc gtc ctg cga ctc atc tag ggagcgttga 1642
Val Lys Ser Val Asp Ser Val Leu Arg Leu Ile
435 440
gctgtgagca tccagcttga ggtgggcacc cgggccaccg tcaccgtccc cggctcctcc 1702
gcgaacctgg gccccggatt cgacaccctc ggcctggccg tgggcatcta cgacaccgtc 1762
gaggtcgagg tcatcgagtc gggcctcgag gtcgaggtct tcggcgaggg cgaggacgac 1822
ctgccccgcg acggttccca cctggtggtc aaggccatcc ggtcgggcct caacgccgcc 1882
cacgccgagg ccccgggcct gcgcgtggtg tgcaccaacg ccatcccgca 1932
<210> 46
<211> 443
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 46
Met Thr Val Thr Gln Gln Pro Ser Phe Asn Pro Gly Lys Gly Ala Gly
1 5 10 15
Glu Pro Val Gly Val Ala Ile Leu Gly His Gly Thr Val Gly Ser Glu
20 25 30
Val Leu Arg Leu Met Gly Glu Tyr Ser Asp Ala Leu Ala His Arg Ile
35 40 45
Gly Gly Pro Leu Glu Leu Lys Gly Val Ala Val Ser Asp Leu Ser Lys
50 55 60
Pro Arg Glu Gly Val Asp Arg Glu Leu Leu Thr Asp Asp Ala Leu Ser
65 70 75 80
Leu Ile Asn Arg Asp Asp Val Asp Ile Val Val Glu Val Ile Gly Gly
85 90 95
Ile Glu Tyr Pro Arg Glu Leu Val Leu Ala Ala Leu Arg Ala Gly Lys
100 105 110
Ser Val Val Thr Ala Asn Lys Ala Leu Val Ala Ala His Ala Asp Glu
115 120 125
Leu Ala Asp Ala Ala Asp Ala Ala Asn Val Asp Leu Tyr Phe Glu Ala
130 135 140
Ala Val Ala Ala Ala Ile Pro Val Val Gly Met Leu Arg Arg Ser Leu
145 150 155 160
Ala Gly Asp Asn Ile Leu Arg Ile Ser Gly Ile Val Asn Gly Thr Thr
165 170 175
Asn Phe Ile Leu Asp Ala Met Glu Ser Thr Gly Ala Ser Tyr Asp Asp
180 185 190
Ala Leu Ala Glu Ala Thr Arg Leu Gly Tyr Ala Glu Ala Asp Pro Thr
195 200 205
Ala Asp Val Glu Gly His Asp Ala Ala Ser Lys Ala Ala Ile Leu Ala
210 215 220
Ser Leu Gly Phe His Thr Arg Val Lys Phe Asp Asp Val Ser Cys Glu
225 230 235 240
Gly Ile Ser Lys Val Thr Ala Glu Asp Ile Glu Ala Ala Arg Asp Ala
245 250 255
Gly Tyr Val Ile Lys Leu Leu Ala Ile Cys Glu Arg Val Thr Asp Lys
260 265 270
Asp Gly Val Glu Ser Val Ala Ser Arg Val His Pro Thr Leu Val Pro
275 280 285
Val Asp His Pro Leu Ala Ser Val Asn Gln Ser Tyr Asn Ala Ile Phe
290 295 300
Val Glu Ala Glu Ala Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala
305 310 315 320
Gly Gly Ala Pro Thr Ala Ser Ala Val Leu Gly Asp Leu Val Gly Ala
325 330 335
Ala Arg Asn Lys Val His Gly Gly Arg Ala Pro Gly Glu Asn Thr Tyr
340 345 350
Ala Asn Leu Pro Ile Ala Asp Leu Gly Asp Val Arg Thr Arg Tyr His
355 360 365
Ile Asp Met Glu Val Glu Asp Arg Thr Gly Val Leu Ala Glu Leu Ser
370 375 380
Ala Ile Phe Ala Thr His Asp Ile Ser Leu Lys Thr Val Arg Gln Glu
385 390 395 400
Glu Ala Gly Asp His Ala Arg Leu Ile Val Val Thr His Ser Ala Arg
405 410 415
Glu Ala Asp Leu Ala Ala Thr Val Glu Asp Leu Lys Lys Ala Pro Ala
420 425 430
Val Lys Ser Val Asp Ser Val Leu Arg Leu Ile
435 440
<210> 47
<211> 1644
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1344)
<223> ген lipA
<400> 47
gcggggtgac catgcacggg ctgagcctga actgctcgaa cacgctcgag cactacgggc 60
acatcgtggc gtgcggcatc gacgacgccg acgtcaccac cctcagcatg gaactggggc 120
gcgacgtcag cgtcggggag gtcacggcac cgcttgtcga cgccctcctg gccgccctcg 180
ccggtgaact cgccgtcgcg gaccacacct tcggatcggc gcccgatccg acgaagattc 240
ccgcgaaaac gcgctgacct ctaagtttat ccctatcagt agaaagagta tggtggcgtt 300
gtg act atc gca cct gaa gga cgc aag ctg ctc cgg gtg gag aag cgc 348
Val Thr Ile Ala Pro Glu Gly Arg Lys Leu Leu Arg Val Glu Lys Arg
1 5 10 15
aat gcg cag acc ccc atc gag acg aag ccc cgt tgg atc cgc aac gcg 396
Asn Ala Gln Thr Pro Ile Glu Thr Lys Pro Arg Trp Ile Arg Asn Ala
20 25 30
gtg aag acc ggc ccc gag tac gag gac atg aag aag aag gtg gcg ggc 444
Val Lys Thr Gly Pro Glu Tyr Glu Asp Met Lys Lys Lys Val Ala Gly
35 40 45
gcc tcc ctg cac acc gtc tgt cag gag gcg ggc tgc ccc aac atc cac 492
Ala Ser Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile His
50 55 60
gag tgc tgg gag tcc cgc gag gcg acg ttt ctc atc ggc ggc gcc aac 540
Glu Cys Trp Glu Ser Arg Glu Ala Thr Phe Leu Ile Gly Gly Ala Asn
65 70 75 80
tgc tcc cgc cgc tgc gac ttc tgc cag atc aac tcc gcg aag ccg gag 588
Cys Ser Arg Arg Cys Asp Phe Cys Gln Ile Asn Ser Ala Lys Pro Glu
85 90 95
ccg ctg gac cgc gac gag ccg cgc cgc gtg gcc gag tcc gtc cgc gag 636
Pro Leu Asp Arg Asp Glu Pro Arg Arg Val Ala Glu Ser Val Arg Glu
100 105 110
atg ggc ctg aac tac tcc acc atc acc ggc gtg acc cgc gac gac ctc 684
Met Gly Leu Asn Tyr Ser Thr Ile Thr Gly Val Thr Arg Asp Asp Leu
115 120 125
gag gac gag ggt gcc tgg ctc tac gcg gag gtc gtc cgc cag atc cac 732
Glu Asp Glu Gly Ala Trp Leu Tyr Ala Glu Val Val Arg Gln Ile His
130 135 140
gag ctc aac ccg aac acc ggc gtc gag aac ctc gtg ccc gac ttc tcc 780
Glu Leu Asn Pro Asn Thr Gly Val Glu Asn Leu Val Pro Asp Phe Ser
145 150 155 160
ggc cgt tcc gag ctg ctg gag cag gtc ttc gag tcc cgc ccc gag gtg 828
Gly Arg Ser Glu Leu Leu Glu Gln Val Phe Glu Ser Arg Pro Glu Val
165 170 175
ttc gcc cac aac ctg gag acc gtg ccg cgc atc ttc aag cgc atc cgc 876
Phe Ala His Asn Leu Glu Thr Val Pro Arg Ile Phe Lys Arg Ile Arg
180 185 190
ccg gcc ttc cgc tac gag cgt tcc ctc gac gtg atc cgc cag gcc cac 924
Pro Ala Phe Arg Tyr Glu Arg Ser Leu Asp Val Ile Arg Gln Ala His
195 200 205
gac tac ggg ctg atc acc aag tcg aac ctc atc ctc ggc atg ggc gag 972
Asp Tyr Gly Leu Ile Thr Lys Ser Asn Leu Ile Leu Gly Met Gly Glu
210 215 220
acc ctc gac gag atc cgt gag gcc ctc acc gac ctg cac agc gcc ggc 1020
Thr Leu Asp Glu Ile Arg Glu Ala Leu Thr Asp Leu His Ser Ala Gly
225 230 235 240
tgc gac atc atc acc atc acg cag tac ctg cgc ccc ggc ccg atg tac 1068
Cys Asp Ile Ile Thr Ile Thr Gln Tyr Leu Arg Pro Gly Pro Met Tyr
245 250 255
cac ccg atc gac cgc tgg gtg aag ccg gag gag ttc gtc gag cac aag 1116
His Pro Ile Asp Arg Trp Val Lys Pro Glu Glu Phe Val Glu His Lys
260 265 270
gac ttc gcc gag gag ctc ggc ttc ggt gcc gtc atg tcc ggc ccg ctg 1164
Asp Phe Ala Glu Glu Leu Gly Phe Gly Ala Val Met Ser Gly Pro Leu
275 280 285
gtc cgt tcc tcc tac cgt gcg gga cgt ctc tac tcg cag gcg atg gcc 1212
Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu Tyr Ser Gln Ala Met Ala
290 295 300
gcc cgc ggc cgg gag atc ccc gac cac ctc aag cac ctg gcc gag acc 1260
Ala Arg Gly Arg Glu Ile Pro Asp His Leu Lys His Leu Ala Glu Thr
305 310 315 320
tcg cag ggc agc acc gac cag gag gcc acc acc ctc ctg gcc aag tac 1308
Ser Gln Gly Ser Thr Asp Gln Glu Ala Thr Thr Leu Leu Ala Lys Tyr
325 330 335
ggc ccc tcc cag gac aca ccg gtc acc tca cgt tag gcacccaccg 1354
Gly Pro Ser Gln Asp Thr Pro Val Thr Ser Arg
340 345
gaccctgacc taaagtagag gccatggcag acgacaagca gaaggcggca gcaaaggccg 1414
cgaaggcaga ggcacgggcc gccaagcggg cgaagagcaa ggagacgcgc gcccagctct 1474
ggcaggcgtt caacatccag cgcaagcagg acaagatgct cgtcccgctg atggtgctct 1534
ccgtcgtggg tgtggccgcg ctcctcttcc tcatcggcct ggcctggggc ggccagtggt 1594
tcacgctcat cctgggtatc ttcctgggtg ccgtcctggc gatgtacatc 1644
<210> 48
<211> 347
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 48
Val Thr Ile Ala Pro Glu Gly Arg Lys Leu Leu Arg Val Glu Lys Arg
1 5 10 15
Asn Ala Gln Thr Pro Ile Glu Thr Lys Pro Arg Trp Ile Arg Asn Ala
20 25 30
Val Lys Thr Gly Pro Glu Tyr Glu Asp Met Lys Lys Lys Val Ala Gly
35 40 45
Ala Ser Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile His
50 55 60
Glu Cys Trp Glu Ser Arg Glu Ala Thr Phe Leu Ile Gly Gly Ala Asn
65 70 75 80
Cys Ser Arg Arg Cys Asp Phe Cys Gln Ile Asn Ser Ala Lys Pro Glu
85 90 95
Pro Leu Asp Arg Asp Glu Pro Arg Arg Val Ala Glu Ser Val Arg Glu
100 105 110
Met Gly Leu Asn Tyr Ser Thr Ile Thr Gly Val Thr Arg Asp Asp Leu
115 120 125
Glu Asp Glu Gly Ala Trp Leu Tyr Ala Glu Val Val Arg Gln Ile His
130 135 140
Glu Leu Asn Pro Asn Thr Gly Val Glu Asn Leu Val Pro Asp Phe Ser
145 150 155 160
Gly Arg Ser Glu Leu Leu Glu Gln Val Phe Glu Ser Arg Pro Glu Val
165 170 175
Phe Ala His Asn Leu Glu Thr Val Pro Arg Ile Phe Lys Arg Ile Arg
180 185 190
Pro Ala Phe Arg Tyr Glu Arg Ser Leu Asp Val Ile Arg Gln Ala His
195 200 205
Asp Tyr Gly Leu Ile Thr Lys Ser Asn Leu Ile Leu Gly Met Gly Glu
210 215 220
Thr Leu Asp Glu Ile Arg Glu Ala Leu Thr Asp Leu His Ser Ala Gly
225 230 235 240
Cys Asp Ile Ile Thr Ile Thr Gln Tyr Leu Arg Pro Gly Pro Met Tyr
245 250 255
His Pro Ile Asp Arg Trp Val Lys Pro Glu Glu Phe Val Glu His Lys
260 265 270
Asp Phe Ala Glu Glu Leu Gly Phe Gly Ala Val Met Ser Gly Pro Leu
275 280 285
Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu Tyr Ser Gln Ala Met Ala
290 295 300
Ala Arg Gly Arg Glu Ile Pro Asp His Leu Lys His Leu Ala Glu Thr
305 310 315 320
Ser Gln Gly Ser Thr Asp Gln Glu Ala Thr Thr Leu Leu Ala Lys Tyr
325 330 335
Gly Pro Ser Gln Asp Thr Pro Val Thr Ser Arg
340 345
<210> 49
<211> 1389
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1089)
<223> ген lipB
<400> 49
gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct acgcggagct 60
cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg agtccacgaa 120
gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca acgacgcggt 180
ccacgacgac tacgccgtga tcaacgacga ccccttcggc gcgggctggc tcttcgccgt 240
cgaggtcgac gaggtcggac cgctcatgac cgccgccgag tacgcggagg ccaacggcgt 300
gtg acc gcc cgg gac cgg tac cct ggt ccc atc atg act gca ccc cgt 348
Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg
1 5 10 15
gag ccc ttc ttc cct gcg gac cgt tcc atc cgt gcc tcc ggc gag ccc 396
Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro
20 25 30
ccg gag atc cgt tat ctc ggc gtc gtc gac tac cag gag gcc tgg aat 444
Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn
35 40 45
ctg cag gcc gag ctg gcg tcg caa cgc gcg gcc ggg gag atc ggt gac 492
Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp
50 55 60
cag ctc ctc atc ctc gag cac ccg agc gtg tac acc gcc ggc aag cgc 540
Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg
65 70 75 80
acg cag ccg gag gac cgc ccc acc aac ggc ctg ccg gtc atc gac gtc 588
Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val
85 90 95
gac cgg ggt ggc cgc atc acc tgg cac ggc gag gga cag ctg gtg gtc 636
Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val
100 105 110
tac ccg atc atc aaa ctg gcc gac ccg gtg gac gtc gtg gac tat gtc 684
Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val
115 120 125
cgc cgc ctc gag gag gcg gtg atc gcg acg gtg cgg gag gtg ggc gtc 732
Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val
130 135 140
ggc aat gcg ggt cgc atc gac ggc cgc tcc gga gtc tgg gtc ccc gcc 780
Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala
145 150 155 160
gac ggg acc cga cgc gac cgg aag gtg gcg gcg ctc ggc atc cgt gtc 828
Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val
165 170 175
acc cgc ggg gtg acc atg cac ggg ctg agc ctg aac tgc tcg aac acg 876
Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr
180 185 190
ctc gag cac tac ggg cac atc gtg gcg tgc ggc atc gac gac gcc gac 924
Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp
195 200 205
gtc acc acc ctc agc atg gaa ctg ggg cgc gac gtc agc gtc ggg gag 972
Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu
210 215 220
gtc acg gca ccg ctt gtc gac gcc ctc ctg gcc gcc ctc gcc ggt gaa 1020
Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu
225 230 235 240
ctc gcc gtc gcg gac cac acc ttc gga tcg gcg ccc gat ccg acg aag 1068
Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys
245 250 255
att ccc gcg aaa acg cgc tga cctctaagtt tatccctatc agtagaaaga 1119
Ile Pro Ala Lys Thr Arg
260
gtatggtggc gttgtgacta tcgcacctga aggacgcaag ctgctccggg tggagaagcg 1179
caatgcgcag acccccatcg agacgaagcc ccgttggatc cgcaacgcgg tgaagaccgg 1239
ccccgagtac gaggacatga agaagaaggt ggcgggcgcc tccctgcaca ccgtctgtca 1299
ggaggcgggc tgccccaaca tccacgagtg ctgggagtcc cgcgaggcga cgtttctcat 1359
cggcggcgcc aactgctccc gccgctgcga 1389
<210> 50
<211> 262
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 50
Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg
1 5 10 15
Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro
20 25 30
Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn
35 40 45
Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp
50 55 60
Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg
65 70 75 80
Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val
85 90 95
Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val
100 105 110
Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val
115 120 125
Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val
130 135 140
Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala
145 150 155 160
Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val
165 170 175
Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr
180 185 190
Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp
195 200 205
Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu
210 215 220
Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu
225 230 235 240
Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys
245 250 255
Ile Pro Ala Lys Thr Arg
260
<210> 51
<211> 2010
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1710)
<223> ген lpd
<400> 51
caactgacat cacagagaga aggcacacat gtccatcttc gagcagatca tcgtcgccat 60
caacaacttc gtcggcccgg gcatccacgc cctgtcctcc gcttcctcca acgccgcact 120
ggccatcggc ctcttctagg tcgaccccgg aagcatctgc ttatcgacgc cccggccccc 180
ggtcggggcg ttcgtcgttg cggcaccaca gtcccgccac cgtcccaccg cccccacggg 240
ggcgggatcc ttttgtgggg ggcggtgaca cgggggaaac ggtcacctat ccttggtggc 300
gtg act gaa cat tat gac gtt gtt gta ctc ggt gcg ggc ccc ggt ggc 348
Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly
1 5 10 15
tac gtc gcc gcc atc cgc gca gcc cag ctc ggc aag aag gtt gct gtc 396
Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val
20 25 30
gtc gag aag cag tac tgg ggc gga gtc tgc ctc aac gtg ggc tgc atc 444
Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile
35 40 45
ccc tcg aag gcg ctg atc aag aac gcc gag atc gcc cac atc ttc acg 492
Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr
50 55 60
cac gag aag aag acg ttc ggc atc aac ggc gac gtc tcc ttc aac tac 540
His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr
65 70 75 80
gag gac gcc cac aag cgc tcc cgt cag gtg tcg gag aag atc gtc ggc 588
Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly
85 90 95
ggc gtc cac tac ctg atg aag aag aac aag atc acc gag atc gac ggc 636
Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly
100 105 110
ttc gga acc ttc agg gac gcc aag acc atc gag gtc agc gag ggc aag 684
Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys
115 120 125
gac gcc ggc aag gtc gtc acc ttc gac gac tgc atc atc gcc acc ggc 732
Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly
130 135 140
tcc gtg gtc aac agc ctc cgt ggc gtc gag ttc tcc gag aac gtc gtc 780
Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val
145 150 155 160
tcc tac gag gag cag atc ctc aac ccg gtc gcc ccg gag aag atg gtc 828
Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val
165 170 175
atc gtc ggt gcc ggc gcc atc ggc atg gag ttc gcc tac gtg ctc tcc 876
Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser
180 185 190
aac tac ggc gtc gac gtc acc gtc atc gag tac atg gac cgt gtc ctg 924
Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu
195 200 205
ccg aac gag gac gag gac gtc tcc aag gtc atc gcc aag gcc tac aag 972
Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys
210 215 220
aag atg ggc gtc acg ctc ctc ccg ggc cac gcc acc acc gcc gtg cgc 1020
Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg
225 230 235 240
gac aac ggc gac tcc gtc gag gtc gac tac cag aag aag ggc tcg gac 1068
Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp
245 250 255
aag acc gag acc atc gtc gcc gac cgc gtc ctc gtc tcc gtc ggc ttc 1116
Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe
260 265 270
cgc ccg cgt gtc gag ggc ttc ggc ctg gag aac acc ggc gtc gag ctc 1164
Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu
275 280 285
acc gag cgc ggt gcc atc gcc atc gac gac ttc atg cgc acc aac gtc 1212
Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val
290 295 300
gag cac atc tac gcc atc ggt gac gtc acc gcc aag ctg cag ctg gcc 1260
Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala
305 310 315 320
cac gtc gcc gag gcg cag ggc atc gtc gcc gcc gag acc atc gcc ggc 1308
His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly
325 330 335
gcc gag acc cac gag ctg ggc gac tac atg atg atg ccg cgc gcc acc 1356
Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr
340 345 350
ttc tgt aac ccg cag gtc gcg tcc ttc ggc tac acc gag gcg cag gcc 1404
Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala
355 360 365
aag gag aag tgg ccg gac cgc gag atc aag gtc gcg tcc ttc ccg ttc 1452
Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe
370 375 380
tcc gcg aac ggc aag gcc gtc ggc ctg gcc gag acc gag ggc ttc gcc 1500
Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala
385 390 395 400
aag atc gtc gcc gac gcc gag tac ggc gag ctg ctg ggc ggc cac ctg 1548
Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu
405 410 415
gtc ggc gcc aac gcg tcc gag ctg ctc aac gag ctg gtc ctc gcg cag 1596
Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln
420 425 430
aac tac gat ctc acc acc gag gag atc agc cgc gcg gtc cac atc cac 1644
Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His
435 440 445
ccg acc ctg tcg gag gcc gtc aag gag gcc gca cac ggc atc gac ggc 1692
Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly
450 455 460
cac atg atc aac ttc tag tcccccggaa aaaggggcct cccggctggg 1740
His Met Ile Asn Phe
465
aggccccttt tctcatgctt tccgacgccc ccggggcgtc gccaagcgcc tagttgtagg 1800
gcgcgaccga cgaccggtgg gcgtcgatgg tgatctcctc gtggatcggc gggaacgcgc 1860
gctgcgcgca gttctcccgc gggcacacgc ggcagccggc gccgatcggg gtggcgctgg 1920
acaggtcggc gaggttgagt ccctgcgcgt agaccgtgcg gtcggcgtgg cgggcctcgc 1980
agcccaggcc gatggcgaag agcttgccgg 2010
<210> 52
<211> 469
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 52
Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly
1 5 10 15
Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val
20 25 30
Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile
35 40 45
Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr
50 55 60
His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr
65 70 75 80
Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly
85 90 95
Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly
100 105 110
Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys
115 120 125
Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly
130 135 140
Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val
145 150 155 160
Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val
165 170 175
Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser
180 185 190
Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu
195 200 205
Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys
210 215 220
Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg
225 230 235 240
Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp
245 250 255
Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe
260 265 270
Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu
275 280 285
Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val
290 295 300
Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala
305 310 315 320
His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly
325 330 335
Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr
340 345 350
Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala
355 360 365
Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe
370 375 380
Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala
385 390 395 400
Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu
405 410 415
Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln
420 425 430
Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His
435 440 445
Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly
450 455 460
His Met Ile Asn Phe
465
<210> 53
<211> 1866
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1566)
<223> ген lysC
<400> 53
gccggtgccc gcccaccaca ggggacggct gatcgcgttc cagaaggggg aacccttgag 60
ggagccgtcg gcgggggcct cctccgcgat gcggtggcgc accacggttc cccacgcgat 120
ggtgagggcg gaggccagtg cgaacagcac ggccagcaga ttgctgtgca cctcatccac 180
cttagtgaac gggggccttt ccgcagggcc gggacccgcc cactcgacta ctcggacgac 240
cccggctccg ggtacgatga agcagaactt tggcacccca gacgaaaggc ggcctacacg 300
gtg gct ctg atc gtt cag aaa tat gga ggc tcc tcc ctg gag acc gcg 348
Val Ala Leu Ile Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Thr Ala
1 5 10 15
gag cgg atc cgc gct gtc gcc gaa cgg atc gtc gcc acg aaa aag gcc 396
Glu Arg Ile Arg Ala Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala
20 25 30
ggc aac gac gtc gtc gtg gtc tgc tcc gcg atg ggc gac acc acg gac 444
Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp
35 40 45
gaa ctc ctg gac ctc gcc gcc cag gtg aac ccg gtt ccg ccg gcc cgt 492
Glu Leu Leu Asp Leu Ala Ala Gln Val Asn Pro Val Pro Pro Ala Arg
50 55 60
gag atg gac atg ctg ctc acc gcg ggt gag cgc atc tcc aac gcg ctg 540
Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu
65 70 75 80
gtc gcg atg gcg atc tcc tcg ctc ggc gcg aag gcg cag tcc ttc acc 588
Val Ala Met Ala Ile Ser Ser Leu Gly Ala Lys Ala Gln Ser Phe Thr
85 90 95
ggc tcg cag gcc ggt gtg ctc acc acc gag cgt cac ggc aac gcc cgc 636
Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg
100 105 110
atc gtc gac gtc acc ccg gac cgc gtc cgc gag gcg ctg gac gag ggc 684
Ile Val Asp Val Thr Pro Asp Arg Val Arg Glu Ala Leu Asp Glu Gly
115 120 125
aac atc tgc atc gtc gcc ggt ttc cag ggc atc aac aag gac acc cgc 732
Asn Ile Cys Ile Val Ala Gly Phe Gln Gly Ile Asn Lys Asp Thr Arg
130 135 140
gac gtc acc acc ctg ggt cgt ggc ggt tcc gac acc aca gcg gtg gcc 780
Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala
145 150 155 160
ctg gcg gcg gcg ctc aac gcc gac gtg tgc gag atc tac tcc gac gtc 828
Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val
165 170 175
gac ggc gtc tac acc gcc gat ccg cgc atc gtc ccg aac gcg cag aag 876
Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys
180 185 190
ctc gag aag ctc tcc ttc gag gag atg ctc gag ctg gcg gcc gtc ggt 924
Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly
195 200 205
tcg aag atc ctg gtg ctg cgt gcc gtc gag tac gca cgt gcc ttc aat 972
Ser Lys Ile Leu Val Leu Arg Ala Val Glu Tyr Ala Arg Ala Phe Asn
210 215 220
gtt ccc ctg cga gtt cgc tcg tct tac agc aat gac acc ggc acc ctg 1020
Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Thr Gly Thr Leu
225 230 235 240
atc gcc ggt tcg atg gag gat atc ccc gtg gaa gaa gca gtt ctc act 1068
Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr
245 250 255
ggc gtt gcc acc gac aag tcc gag gcc aag gtc acc atc ctg ggt att 1116
Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Ile Leu Gly Ile
260 265 270
ccc gac aag ccg ggc gag gcg gcc acc gtc ttc cgc gcc gtc gcg gac 1164
Pro Asp Lys Pro Gly Glu Ala Ala Thr Val Phe Arg Ala Val Ala Asp
275 280 285
gcg gag atc aac atc gac atg gtc ctg cag aac gtc tcc tcc ctg gag 1212
Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Leu Glu
290 295 300
gac ggc acg acc gac atc acc ttc acc tgc ccg cgt gcc gac ggc ccc 1260
Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ala Asp Gly Pro
305 310 315 320
cgc gcc atc gag ctg ctc aag aag atg cag gcg aag agc ggc tgg tcc 1308
Arg Ala Ile Glu Leu Leu Lys Lys Met Gln Ala Lys Ser Gly Trp Ser
325 330 335
aac gtg ctc tac gac gac cag gtg ggc aag gtc tcc ctc gtg ggt gcc 1356
Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala
340 345 350
ggc atg aag tcc cac ccg ggc gtc acc gcc gac ttc gcc gag gcg ctc 1404
Gly Met Lys Ser His Pro Gly Val Thr Ala Asp Phe Ala Glu Ala Leu
355 360 365
cgc gac gcc ggc gtg aac atc gag cta atc tcc acc tcg gag atc cgc 1452
Arg Asp Ala Gly Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg
370 375 380
atc tcg gtg ctc acc cgc gag gcc gac ctg gac gcc gcc gcc cgt gcc 1500
Ile Ser Val Leu Thr Arg Glu Ala Asp Leu Asp Ala Ala Ala Arg Ala
385 390 395 400
ctg cac gag aag ttc gag ctc ggc ggc gac gcc gag gcc acc gtc tac 1548
Leu His Glu Lys Phe Glu Leu Gly Gly Asp Ala Glu Ala Thr Val Tyr
405 410 415
gcc ggc acc ggc cgc tag acaatccctt ccacagagga gctttcactc 1596
Ala Gly Thr Gly Arg
420
tcatgaccac cattgctgtc gtcggtgcca ccggccaggt cggccgcgtg atgcgctcga 1656
tcctggagga gcgcaacttc ccggccgaca aggtccgttt cttcgcgtcc ccgcgctccg 1716
cgggcaccgt catcgagttc cgcggtgagg acatcaccgt cgaggacctc acccagatca 1776
ccccggagtc cgtcaaggac gtcgacatcg ccctcttctc cgccggtggt tccacctccc 1836
gtgagtgggc cccggtcttc gccgaggccg 1866
<210> 54
<211> 421
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 54
Val Ala Leu Ile Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Thr Ala
1 5 10 15
Glu Arg Ile Arg Ala Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala
20 25 30
Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp
35 40 45
Glu Leu Leu Asp Leu Ala Ala Gln Val Asn Pro Val Pro Pro Ala Arg
50 55 60
Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu
65 70 75 80
Val Ala Met Ala Ile Ser Ser Leu Gly Ala Lys Ala Gln Ser Phe Thr
85 90 95
Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg
100 105 110
Ile Val Asp Val Thr Pro Asp Arg Val Arg Glu Ala Leu Asp Glu Gly
115 120 125
Asn Ile Cys Ile Val Ala Gly Phe Gln Gly Ile Asn Lys Asp Thr Arg
130 135 140
Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala
145 150 155 160
Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val
165 170 175
Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys
180 185 190
Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly
195 200 205
Ser Lys Ile Leu Val Leu Arg Ala Val Glu Tyr Ala Arg Ala Phe Asn
210 215 220
Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Thr Gly Thr Leu
225 230 235 240
Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr
245 250 255
Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Ile Leu Gly Ile
260 265 270
Pro Asp Lys Pro Gly Glu Ala Ala Thr Val Phe Arg Ala Val Ala Asp
275 280 285
Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Leu Glu
290 295 300
Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ala Asp Gly Pro
305 310 315 320
Arg Ala Ile Glu Leu Leu Lys Lys Met Gln Ala Lys Ser Gly Trp Ser
325 330 335
Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala
340 345 350
Gly Met Lys Ser His Pro Gly Val Thr Ala Asp Phe Ala Glu Ala Leu
355 360 365
Arg Asp Ala Gly Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg
370 375 380
Ile Ser Val Leu Thr Arg Glu Ala Asp Leu Asp Ala Ala Ala Arg Ala
385 390 395 400
Leu His Glu Lys Phe Glu Leu Gly Gly Asp Ala Glu Ala Thr Val Tyr
405 410 415
Ala Gly Thr Gly Arg
420
<210> 55
<211> 1794
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1494)
<223> ген metB
<400> 55
acctccccgg acgggagaga ctgctcggtg tgatcgaccg gctccgcgcg gcgcagcccg 60
gcctgacgac ggtgctggtg acgcatcacg tggaggagat cgcggcgtcg acaagcgatg 120
tcctgctgct caaggagggc cgcgtcctgg cctccggacc cgtcgacgag acgctgacct 180
cggggaacct gtcggcactc tacgacctgc cggtgtcgtt ggagaaggtc cacggacgct 240
ggttcgcgct ctgcatataa ttcccggatg ctgccgggaa agtgaaccgc ttggtctata 300
gtg gcc gac atg acc cac gga ctt tct ggt ttc tcg tct cgt tcc atc 348
Val Ala Asp Met Thr His Gly Leu Ser Gly Phe Ser Ser Arg Ser Ile
1 5 10 15
cat gcc gga tac gag cct gac agc ctc tac ggc ccg atc aac acg ccg 396
His Ala Gly Tyr Glu Pro Asp Ser Leu Tyr Gly Pro Ile Asn Thr Pro
20 25 30
atc tac gcg tcg acc acc ttc gcg cag gac ggc ctc aac gag ctg cgc 444
Ile Tyr Ala Ser Thr Thr Phe Ala Gln Asp Gly Leu Asn Glu Leu Arg
35 40 45
ggt ggc ttc gag tac acc cgc tgc ggc aac ccc acg atc gac gcc ctc 492
Gly Gly Phe Glu Tyr Thr Arg Cys Gly Asn Pro Thr Ile Asp Ala Leu
50 55 60
gaa aag acc gtc gca gcc ctg gaa ggc gca cag tac ggc cgc gcg ttc 540
Glu Lys Thr Val Ala Ala Leu Glu Gly Ala Gln Tyr Gly Arg Ala Phe
65 70 75 80
tcc tcc ggc atg gcc gcc acc gac gtc atc ctg cgt atc ctc ctg cgc 588
Ser Ser Gly Met Ala Ala Thr Asp Val Ile Leu Arg Ile Leu Leu Arg
85 90 95
ccc ggt gac cac ctc atc ctc ggc cac gac gcc tac ggc ggc acc tgg 636
Pro Gly Asp His Leu Ile Leu Gly His Asp Ala Tyr Gly Gly Thr Trp
100 105 110
cgc ctc atc gac cag gcc ttc ggc cag tgg ggc gtc gag tac acc gtc 684
Arg Leu Ile Asp Gln Ala Phe Gly Gln Trp Gly Val Glu Tyr Thr Val
115 120 125
gtc gac acc acc gac gtt gcc gcc gtc gag gcc gcc ctc cgc ccg aac 732
Val Asp Thr Thr Asp Val Ala Ala Val Glu Ala Ala Leu Arg Pro Asn
130 135 140
acc aag ctc atc tgg ctg gag acc ccg acc aac ccg gcc ctg tcc atc 780
Thr Lys Leu Ile Trp Leu Glu Thr Pro Thr Asn Pro Ala Leu Ser Ile
145 150 155 160
acc gac atc gcc gcc atc gcc gcg atc aag cag cag gcc gcc ctc gtc 828
Thr Asp Ile Ala Ala Ile Ala Ala Ile Lys Gln Gln Ala Ala Leu Val
165 170 175
gtg gac aac acc ttc gcg tcc ccg tac ctg cag cag ccg ctc gcc ctc 876
Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Ala Leu
180 185 190
ggc gcg gac cac gtc ctg cac tcc gcc acc aag tac ctc ggc ggt cac 924
Gly Ala Asp His Val Leu His Ser Ala Thr Lys Tyr Leu Gly Gly His
195 200 205
tcc gac gtc gtc ggc ggc ctc gtg gtg acc aac tgc gcc gag ttc gac 972
Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Cys Ala Glu Phe Asp
210 215 220
gag cag ctc ctc tgg ttc cag ggc ggc gtc ggc ccc atc ccg tcc ccc 1020
Glu Gln Leu Leu Trp Phe Gln Gly Gly Val Gly Pro Ile Pro Ser Pro
225 230 235 240
ttc gac gcc tac ctc acc gcc cgc ggc atc aag acc ctg gcc gtg cgc 1068
Phe Asp Ala Tyr Leu Thr Ala Arg Gly Ile Lys Thr Leu Ala Val Arg
245 250 255
atg gag cgc cac tgc gac aac gcc gag gcc atc gcc gcg cac ctc gag 1116
Met Glu Arg His Cys Asp Asn Ala Glu Ala Ile Ala Ala His Leu Glu
260 265 270
gcc tcc gac cag gtc gcg acc gtg ctc tac ccc ggc ctc gag tcc cac 1164
Ala Ser Asp Gln Val Ala Thr Val Leu Tyr Pro Gly Leu Glu Ser His
275 280 285
ccg ggc cac gag gtg gcc aag cgc cag atg aag cgc ttc ggc ggc atg 1212
Pro Gly His Glu Val Ala Lys Arg Gln Met Lys Arg Phe Gly Gly Met
290 295 300
atc tcc gtg cgc ttc cgc tcc gag gag gcc gcc cgc gtc ttc tgc ctg 1260
Ile Ser Val Arg Phe Arg Ser Glu Glu Ala Ala Arg Val Phe Cys Leu
305 310 315 320
aac acc cga ctg atc tgt ctg gcc gag tct ctc ggc ggc gtc gag tcg 1308
Asn Thr Arg Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser
325 330 335
ctc gtc gag cac ccg gcc acc atg acc cac cag tcc gcc acc ggc tcc 1356
Leu Val Glu His Pro Ala Thr Met Thr His Gln Ser Ala Thr Gly Ser
340 345 350
cag ctg gag gtc ccg cgt gac ctg gtg cgc atc tcc atc ggc atc gag 1404
Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu
355 360 365
gac gtc gag gac ctc atc gcg gac atc gac gtc gcc ctc cag gcc gtc 1452
Asp Val Glu Asp Leu Ile Ala Asp Ile Asp Val Ala Leu Gln Ala Val
370 375 380
gcg gcc gcg gag gcg gcc gct gag gcc tcc gtc gac gcc tga 1494
Ala Ala Ala Glu Ala Ala Ala Glu Ala Ser Val Asp Ala
385 390 395
gtgatgaccg ccccgcacac ggtcgagagg acatgtgcgg gacggctcct cccgggcggc 1554
gcagtgtcga gacgggtact cctcccgaac atgtgagccc gcccctggct ccggccccct 1614
cagggcagcc ggatcgccgc cacaccgacc accatgagcg ccaggcccac cgcctggacc 1674
agggtggtcg gtttcctctt cgcccccagc agcccgaagt ggtcgatgag gaggctgccg 1734
accatcatgc cgagcaggat cgtcatgacg gtcagacccg tgccgatgac gggggagagg 1794
<210> 56
<211> 397
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 56
Val Ala Asp Met Thr His Gly Leu Ser Gly Phe Ser Ser Arg Ser Ile
1 5 10 15
His Ala Gly Tyr Glu Pro Asp Ser Leu Tyr Gly Pro Ile Asn Thr Pro
20 25 30
Ile Tyr Ala Ser Thr Thr Phe Ala Gln Asp Gly Leu Asn Glu Leu Arg
35 40 45
Gly Gly Phe Glu Tyr Thr Arg Cys Gly Asn Pro Thr Ile Asp Ala Leu
50 55 60
Glu Lys Thr Val Ala Ala Leu Glu Gly Ala Gln Tyr Gly Arg Ala Phe
65 70 75 80
Ser Ser Gly Met Ala Ala Thr Asp Val Ile Leu Arg Ile Leu Leu Arg
85 90 95
Pro Gly Asp His Leu Ile Leu Gly His Asp Ala Tyr Gly Gly Thr Trp
100 105 110
Arg Leu Ile Asp Gln Ala Phe Gly Gln Trp Gly Val Glu Tyr Thr Val
115 120 125
Val Asp Thr Thr Asp Val Ala Ala Val Glu Ala Ala Leu Arg Pro Asn
130 135 140
Thr Lys Leu Ile Trp Leu Glu Thr Pro Thr Asn Pro Ala Leu Ser Ile
145 150 155 160
Thr Asp Ile Ala Ala Ile Ala Ala Ile Lys Gln Gln Ala Ala Leu Val
165 170 175
Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Ala Leu
180 185 190
Gly Ala Asp His Val Leu His Ser Ala Thr Lys Tyr Leu Gly Gly His
195 200 205
Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Cys Ala Glu Phe Asp
210 215 220
Glu Gln Leu Leu Trp Phe Gln Gly Gly Val Gly Pro Ile Pro Ser Pro
225 230 235 240
Phe Asp Ala Tyr Leu Thr Ala Arg Gly Ile Lys Thr Leu Ala Val Arg
245 250 255
Met Glu Arg His Cys Asp Asn Ala Glu Ala Ile Ala Ala His Leu Glu
260 265 270
Ala Ser Asp Gln Val Ala Thr Val Leu Tyr Pro Gly Leu Glu Ser His
275 280 285
Pro Gly His Glu Val Ala Lys Arg Gln Met Lys Arg Phe Gly Gly Met
290 295 300
Ile Ser Val Arg Phe Arg Ser Glu Glu Ala Ala Arg Val Phe Cys Leu
305 310 315 320
Asn Thr Arg Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser
325 330 335
Leu Val Glu His Pro Ala Thr Met Thr His Gln Ser Ala Thr Gly Ser
340 345 350
Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu
355 360 365
Asp Val Glu Asp Leu Ile Ala Asp Ile Asp Val Ala Leu Gln Ala Val
370 375 380
Ala Ala Ala Glu Ala Ala Ala Glu Ala Ser Val Asp Ala
385 390 395
<210> 57
<211> 1581
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1281)
<223> ген metF
<400> 57
ggttcacggt gtgctccttg ttcagtgggt cattctccct cccagactag cggcgaagcc 60
tcgatcaggg cgggtttccg gatgtgtgga acctggggaa atgaaactga accgcttggt 120
ccatttttgt ccgaaaatcg gccctcgggg gtgaattata ttcatgctag ggtggggtta 180
tcgccgcagg tcacgcggca aaccggaaat agcaccttta ggaaaattac ccgccgcaaa 240
ccgttggctt gctacattca cagccacttg cccaacggga tgtctgaagg aggaaaacac 300
atg aac gca aac gcg ccg cag tac agc gcc aag cgt cga ttc tcc gcc 348
Met Asn Ala Asn Ala Pro Gln Tyr Ser Ala Lys Arg Arg Phe Ser Ala
1 5 10 15
tcc gcc ccg ttg gac acc ttc gat ccc gag gaa tgg cgc cag gcc ccc 396
Ser Ala Pro Leu Asp Thr Phe Asp Pro Glu Glu Trp Arg Gln Ala Pro
20 25 30
gtg ccg gag cgc acc tcc ctc tcc ttc gag gtc atg ccc ccg cgc cac 444
Val Pro Glu Arg Thr Ser Leu Ser Phe Glu Val Met Pro Pro Arg His
35 40 45
gac gcg gac gag gcc aag atc gac gaa ctg ctg gac acg ctg cag tcc 492
Asp Ala Asp Glu Ala Lys Ile Asp Glu Leu Leu Asp Thr Leu Gln Ser
50 55 60
tac aac ccg gac tac atc tcc gtc acc agc tcc cgg aac tcc ggc tgg 540
Tyr Asn Pro Asp Tyr Ile Ser Val Thr Ser Ser Arg Asn Ser Gly Trp
65 70 75 80
ctc gag ggc acc gcg aag ttc atc gcg aag atc agc gcc cag acc cgc 588
Leu Glu Gly Thr Ala Lys Phe Ile Ala Lys Ile Ser Ala Gln Thr Arg
85 90 95
atc ccg acg gtc gcc cac ctg gcc tgc acc gcc ggc acc cgc gag gag 636
Ile Pro Thr Val Ala His Leu Ala Cys Thr Ala Gly Thr Arg Glu Glu
100 105 110
ctc atc ggc tgg atc aac cgg ctc atg gac tcc ggc gtc cgc ggc ctg 684
Leu Ile Gly Trp Ile Asn Arg Leu Met Asp Ser Gly Val Arg Gly Leu
115 120 125
ctc gcc ctc cgc ggc gac ctc gca gaa gga cag acc ggt atg cct gag 732
Leu Ala Leu Arg Gly Asp Leu Ala Glu Gly Gln Thr Gly Met Pro Glu
130 135 140
ggt tac ctc aag cac gcg acc gac ctc atc aac ctc atc cag gag atc 780
Gly Tyr Leu Lys His Ala Thr Asp Leu Ile Asn Leu Ile Gln Glu Ile
145 150 155 160
gag gag gag cag gtc gcc cgc ttc ggc gcc ggc cgc ctc gcc atc ggc 828
Glu Glu Glu Gln Val Ala Arg Phe Gly Ala Gly Arg Leu Ala Ile Gly
165 170 175
gtc gcc gcc tac ccc agc ggc cac gag gag tcg acc agc cgc gac gag 876
Val Ala Ala Tyr Pro Ser Gly His Glu Glu Ser Thr Ser Arg Asp Glu
180 185 190
gac atc gac gtc ctc ctg gcc aag cag cgc ctg ggc gcc gac ttc gcc 924
Asp Ile Asp Val Leu Leu Ala Lys Gln Arg Leu Gly Ala Asp Phe Ala
195 200 205
atc acc cag ctc ttc ttc gac gcc gag gac tac ctc cgc ttc ctg gag 972
Ile Thr Gln Leu Phe Phe Asp Ala Glu Asp Tyr Leu Arg Phe Leu Glu
210 215 220
cgc gcc cgc ctg gca ggc gtc cgc atc ccg ctc atc ccc ggc atc atg 1020
Arg Ala Arg Leu Ala Gly Val Arg Ile Pro Leu Ile Pro Gly Ile Met
225 230 235 240
ccg atg acg agc gtc gcg aga cta cgt cga atg gga cag ctc tcc ggc 1068
Pro Met Thr Ser Val Ala Arg Leu Arg Arg Met Gly Gln Leu Ser Gly
245 250 255
ctt acc gtc ccc gac cgg ctg atc agc cga ctg gag gcg gca ggc ccg 1116
Leu Thr Val Pro Asp Arg Leu Ile Ser Arg Leu Glu Ala Ala Gly Pro
260 265 270
gag agc gag tac cag acg ggc ctc gac ctc acg gcc gag ctc gcg cag 1164
Glu Ser Glu Tyr Gln Thr Gly Leu Asp Leu Thr Ala Glu Leu Ala Gln
275 280 285
acc atc ctg gac gcc ggt gcc ggg ggg atg cac gtg tac acg ttc aac 1212
Thr Ile Leu Asp Ala Gly Ala Gly Gly Met His Val Tyr Thr Phe Asn
290 295 300
cgg ccg gac acc acc acc gaa ctg ctg gac cgc atc ggc atc acc ccc 1260
Arg Pro Asp Thr Thr Thr Glu Leu Leu Asp Arg Ile Gly Ile Thr Pro
305 310 315 320
gac cct cgg gtt agg gag tga ggcgggagac cgccgccgct gcatctcatg 1311
Asp Pro Arg Val Arg Glu
325
ttgatagcga cctgctgcac gcaccgggag cccccgaagc agaccaggtt gtacacccaa 1371
gattttccga aaggcctccc ttttcatgtc ttccaccgct ttccccacct tcaccatcga 1431
gggctacccg cgcgtcggcg ccaaccgcga gctcaagaag gccctcgagt ccttctgggc 1491
cggccgcatc gacgaggaga ccttcatctc ctccgcgcac tccatccgcc tggagaacta 1551
cgcccgtctg cgtgacctcg gcctggacca 1581
<210> 58
<211> 326
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 58
Met Asn Ala Asn Ala Pro Gln Tyr Ser Ala Lys Arg Arg Phe Ser Ala
1 5 10 15
Ser Ala Pro Leu Asp Thr Phe Asp Pro Glu Glu Trp Arg Gln Ala Pro
20 25 30
Val Pro Glu Arg Thr Ser Leu Ser Phe Glu Val Met Pro Pro Arg His
35 40 45
Asp Ala Asp Glu Ala Lys Ile Asp Glu Leu Leu Asp Thr Leu Gln Ser
50 55 60
Tyr Asn Pro Asp Tyr Ile Ser Val Thr Ser Ser Arg Asn Ser Gly Trp
65 70 75 80
Leu Glu Gly Thr Ala Lys Phe Ile Ala Lys Ile Ser Ala Gln Thr Arg
85 90 95
Ile Pro Thr Val Ala His Leu Ala Cys Thr Ala Gly Thr Arg Glu Glu
100 105 110
Leu Ile Gly Trp Ile Asn Arg Leu Met Asp Ser Gly Val Arg Gly Leu
115 120 125
Leu Ala Leu Arg Gly Asp Leu Ala Glu Gly Gln Thr Gly Met Pro Glu
130 135 140
Gly Tyr Leu Lys His Ala Thr Asp Leu Ile Asn Leu Ile Gln Glu Ile
145 150 155 160
Glu Glu Glu Gln Val Ala Arg Phe Gly Ala Gly Arg Leu Ala Ile Gly
165 170 175
Val Ala Ala Tyr Pro Ser Gly His Glu Glu Ser Thr Ser Arg Asp Glu
180 185 190
Asp Ile Asp Val Leu Leu Ala Lys Gln Arg Leu Gly Ala Asp Phe Ala
195 200 205
Ile Thr Gln Leu Phe Phe Asp Ala Glu Asp Tyr Leu Arg Phe Leu Glu
210 215 220
Arg Ala Arg Leu Ala Gly Val Arg Ile Pro Leu Ile Pro Gly Ile Met
225 230 235 240
Pro Met Thr Ser Val Ala Arg Leu Arg Arg Met Gly Gln Leu Ser Gly
245 250 255
Leu Thr Val Pro Asp Arg Leu Ile Ser Arg Leu Glu Ala Ala Gly Pro
260 265 270
Glu Ser Glu Tyr Gln Thr Gly Leu Asp Leu Thr Ala Glu Leu Ala Gln
275 280 285
Thr Ile Leu Asp Ala Gly Ala Gly Gly Met His Val Tyr Thr Phe Asn
290 295 300
Arg Pro Asp Thr Thr Thr Glu Leu Leu Asp Arg Ile Gly Ile Thr Pro
305 310 315 320
Asp Pro Arg Val Arg Glu
325
<210> 59
<211> 1695
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1395)
<223> ген metX
<400> 59
tccgcctgtc ggtgggcatc gagaagatca ccgacatcat cgccgacctg gagaacgccc 60
tgaacgccgt ctaagagaca gcttggtcca catacattca cgtcccccac ctggggagac 120
gtggatgtcg gcgacccttc tgtttgcgtg gtcacgcgcg gaaacctacc ctttcaggag 180
accgtgccga atgtaggcac cgaggacgac aaactgtccc ccgggacccc caccagacca 240
ctaggacggt gagtttccat ctctaccctc aacctggccc cccagggcga actcgcgcag 300
gtg cgc atc ggt gat ctc cac acg gag gcc ggt ggc gtc atc aac gac 348
Val Arg Ile Gly Asp Leu His Thr Glu Ala Gly Gly Val Ile Asn Asp
1 5 10 15
gtc acc atc gcc tac cgc cgc tgg ggc gag ttc cgg gga gac ccg gac 396
Val Thr Ile Ala Tyr Arg Arg Trp Gly Glu Phe Arg Gly Asp Pro Asp
20 25 30
ggg atg aac aac ctc gtg ctc atc gaa cac gcc ctc acc ggc gac acc 444
Gly Met Asn Asn Leu Val Leu Ile Glu His Ala Leu Thr Gly Asp Thr
35 40 45
aac gtc gcc gac tgg tgg tgt gac ctg ctc ggc ccc ggc cgg gcg ctc 492
Asn Val Ala Asp Trp Trp Cys Asp Leu Leu Gly Pro Gly Arg Ala Leu
50 55 60
gac acc gac gcg tgg tgc atc gtg tgc acc aac gtc atc ggc ggc tgc 540
Asp Thr Asp Ala Trp Cys Ile Val Cys Thr Asn Val Ile Gly Gly Cys
65 70 75 80
cag ggc tcg acc ggc ccc gcc tcc gaa cac ccc gac ggc ggc cgc tgg 588
Gln Gly Ser Thr Gly Pro Ala Ser Glu His Pro Asp Gly Gly Arg Trp
85 90 95
gga tcc cgc ttc ccg gcg atc tcc atc cgc gac cag gtc acc gcg gag 636
Gly Ser Arg Phe Pro Ala Ile Ser Ile Arg Asp Gln Val Thr Ala Glu
100 105 110
aag cag ttc ctc gac gcc ctc ggc gtc tcc cgc atc cac gcc gtc ctc 684
Lys Gln Phe Leu Asp Ala Leu Gly Val Ser Arg Ile His Ala Val Leu
115 120 125
ggc ggt tcc atg ggc ggt gcc cgc acc ctc gag tgg gcg atg atg tac 732
Gly Gly Ser Met Gly Gly Ala Arg Thr Leu Glu Trp Ala Met Met Tyr
130 135 140
ccg gac aag gtc aac gcc gtg tgc gtc atc gcc gtc tcc gcc cgg gcc 780
Pro Asp Lys Val Asn Ala Val Cys Val Ile Ala Val Ser Ala Arg Ala
145 150 155 160
agc gcc tgg cag atc ggc atc cag tcc gcg cag atc tcc gcc atc gag 828
Ser Ala Trp Gln Ile Gly Ile Gln Ser Ala Gln Ile Ser Ala Ile Glu
165 170 175
cgt gac ccc gcc tgg cag ggc ggc gac ttc tac gac acc ggt gcc tcc 876
Arg Asp Pro Ala Trp Gln Gly Gly Asp Phe Tyr Asp Thr Gly Ala Ser
180 185 190
ccc gac gcc ggc ctc gcc gcc gcc cgc cgc atc gcc cac ctc acc tac 924
Pro Asp Ala Gly Leu Ala Ala Ala Arg Arg Ile Ala His Leu Thr Tyr
195 200 205
cgc ggt gag cag gag ctc gac gag cgt ttc ggc gcc gcc cgc cag gac 972
Arg Gly Glu Gln Glu Leu Asp Glu Arg Phe Gly Ala Ala Arg Gln Asp
210 215 220
ggg gag aac ccg ctg ggc acc ttc cgg gac ccc gcc cag cgt ttc gcc 1020
Gly Glu Asn Pro Leu Gly Thr Phe Arg Asp Pro Ala Gln Arg Phe Ala
225 230 235 240
gtc gag agc tac ctg cag tac cac ggc cgc aag ctg gtc gag cgt ttc 1068
Val Glu Ser Tyr Leu Gln Tyr His Gly Arg Lys Leu Val Glu Arg Phe
245 250 255
gac gcc ggc agc tac gtc acc ctc acc gag gcc ctc aac cgg cac aac 1116
Asp Ala Gly Ser Tyr Val Thr Leu Thr Glu Ala Leu Asn Arg His Asn
260 265 270
atc ggc cgg ggc cgc ggg ggc ctg aac aag gcg ctg gcg gcc tgc acg 1164
Ile Gly Arg Gly Arg Gly Gly Leu Asn Lys Ala Leu Ala Ala Cys Thr
275 280 285
gtg ccg acg atg atc gtc ggc gtc gac acg gac atc ctg tac ccg tac 1212
Val Pro Thr Met Ile Val Gly Val Asp Thr Asp Ile Leu Tyr Pro Tyr
290 295 300
cac cag cag gag cac ctc tcc cgc aac gtc ggt gag ctg atg ggc atc 1260
His Gln Gln Glu His Leu Ser Arg Asn Val Gly Glu Leu Met Gly Ile
305 310 315 320
gcc cgg atc tcc tcc cct gtc ggc cac gac gcc ttc ctg gcg gag ttc 1308
Ala Arg Ile Ser Ser Pro Val Gly His Asp Ala Phe Leu Ala Glu Phe
325 330 335
cgg cag atg gac cgc atc ctc cgg cgc ttc ttc gtc ctc agc gtg ctg 1356
Arg Gln Met Asp Arg Ile Leu Arg Arg Phe Phe Val Leu Ser Val Leu
340 345 350
gac gag gac gcc cag gag gac gac ggc tac ctc atc tag caacccgaac 1405
Asp Glu Asp Ala Gln Glu Asp Asp Gly Tyr Leu Ile
355 360
gtgccgcgag ggggctccca gccatcggtt gggagccccc tcgcggcacg tttcgcgggg 1465
tcacgagccc agcgcgtcca ccgaggccgc caggaacgcc atcgtccccc cgaggacggt 1525
gcagaaggcg gcgtcgaaac gccgggagcg cacggcgaac accccgacga tgcgggagtc 1585
gcacgcgtag cgcaccacgg ccagccacat gagtgagacg ccgagcacga acgtcgcgcg 1645
gcgccagtgc tcggtgagcg cgaagacgct cgccacgacc acgccgagca 1695
<210> 60
<211> 364
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 60
Val Arg Ile Gly Asp Leu His Thr Glu Ala Gly Gly Val Ile Asn Asp
1 5 10 15
Val Thr Ile Ala Tyr Arg Arg Trp Gly Glu Phe Arg Gly Asp Pro Asp
20 25 30
Gly Met Asn Asn Leu Val Leu Ile Glu His Ala Leu Thr Gly Asp Thr
35 40 45
Asn Val Ala Asp Trp Trp Cys Asp Leu Leu Gly Pro Gly Arg Ala Leu
50 55 60
Asp Thr Asp Ala Trp Cys Ile Val Cys Thr Asn Val Ile Gly Gly Cys
65 70 75 80
Gln Gly Ser Thr Gly Pro Ala Ser Glu His Pro Asp Gly Gly Arg Trp
85 90 95
Gly Ser Arg Phe Pro Ala Ile Ser Ile Arg Asp Gln Val Thr Ala Glu
100 105 110
Lys Gln Phe Leu Asp Ala Leu Gly Val Ser Arg Ile His Ala Val Leu
115 120 125
Gly Gly Ser Met Gly Gly Ala Arg Thr Leu Glu Trp Ala Met Met Tyr
130 135 140
Pro Asp Lys Val Asn Ala Val Cys Val Ile Ala Val Ser Ala Arg Ala
145 150 155 160
Ser Ala Trp Gln Ile Gly Ile Gln Ser Ala Gln Ile Ser Ala Ile Glu
165 170 175
Arg Asp Pro Ala Trp Gln Gly Gly Asp Phe Tyr Asp Thr Gly Ala Ser
180 185 190
Pro Asp Ala Gly Leu Ala Ala Ala Arg Arg Ile Ala His Leu Thr Tyr
195 200 205
Arg Gly Glu Gln Glu Leu Asp Glu Arg Phe Gly Ala Ala Arg Gln Asp
210 215 220
Gly Glu Asn Pro Leu Gly Thr Phe Arg Asp Pro Ala Gln Arg Phe Ala
225 230 235 240
Val Glu Ser Tyr Leu Gln Tyr His Gly Arg Lys Leu Val Glu Arg Phe
245 250 255
Asp Ala Gly Ser Tyr Val Thr Leu Thr Glu Ala Leu Asn Arg His Asn
260 265 270
Ile Gly Arg Gly Arg Gly Gly Leu Asn Lys Ala Leu Ala Ala Cys Thr
275 280 285
Val Pro Thr Met Ile Val Gly Val Asp Thr Asp Ile Leu Tyr Pro Tyr
290 295 300
His Gln Gln Glu His Leu Ser Arg Asn Val Gly Glu Leu Met Gly Ile
305 310 315 320
Ala Arg Ile Ser Ser Pro Val Gly His Asp Ala Phe Leu Ala Glu Phe
325 330 335
Arg Gln Met Asp Arg Ile Leu Arg Arg Phe Phe Val Leu Ser Val Leu
340 345 350
Asp Glu Asp Ala Gln Glu Asp Asp Gly Tyr Leu Ile
355 360
<210> 61
<211> 1872
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1572)
<223> ген metY
<400> 61
ccaagttgga gcggcgcggc atcaagttcg cgatggcgcg cgtgaagcag gacctctacc 60
gacgcctcga gcccaccggc ttcatcgacc acgtcggccg ggactacatt ttcgagacgc 120
tgcccaccgg ggtgcgggcc tacgccaacc gctaccaggc ccggcacgga cgctggccgg 180
acggggtgcc gcgcgaggtg gtcgagccct gacgcggacg gggtggaccc cgagctcatg 240
cccgcggcgg gtacatttgc attcaggtat tccgtcgccc cgaaaggtgt ctgcatcacc 300
atg cac aac cag acc aag ctc atc cac tcc ggc tat gtc ccg ggc aac 348
Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn
1 5 10 15
aag gac ccc cga cag gtg ccg atc gtg cag tcc acg acc tac acc ttc 396
Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe
20 25 30
gac tcc tcc gag gac atc gcg gcg gtg ttc gat gag ccg acc cac gcg 444
Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala
35 40 45
ctg atc tac tcc cgc ttc gcc aac ccg acc gtc atg gcg gtg gag acg 492
Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr
50 55 60
aag atc gcc gag ctg gag ggc ggt gcc gcg gcg atg gcg acc tcc tcc 540
Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser
65 70 75 80
ggc cag gcc gcg acc gcg ctg tcg atc atg aac gtc tgc tcg gcg ggt 588
Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly
85 90 95
gac tcc ttc gtg gcc tcc tcc gag atc tac ggc ggc acc ctc aac ctc 636
Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu
100 105 110
ttc cac acg acg ctc aag cgt ttc ggc atc gag gtc atc tac gtc gac 684
Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp
115 120 125
cag gac gcc tcc gag gag gag atc gcc gcc gcc ttc aag ccg aac acg 732
Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr
130 135 140
aag gca ctg ttc ggt gag acc atc ggc aac ccg gcg atg agc gtg ctg 780
Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu
145 150 155 160
gac atc gag aag ttc gcc cgc atc gcc cac ggt cag ggc gtg ccg ctc 828
Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu
165 170 175
atc gtc gac tcc acc ttc gcc acc ccg atg ctg tgc cgc ccc atc gag 876
Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu
180 185 190
cac ggc gcc gac gtg gtc atc cac tcc acc tcc aag tac atg gac ggc 924
His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly
195 200 205
cac gcg ctg cag gtc ggc ggc atg atc gtc gac gcc ggc acc ttc gac 972
His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp
210 215 220
tac acc aac ggc aag ttc ccg ggc ttc acc gag ccg gac gag tcc tac 1020
Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr
225 230 235 240
cac ggc gtc gtg tac acc aag gac tac gcc gtt gcc ccc ttc atc atc 1068
His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile
245 250 255
aag gcc cgc atg cag ctg cag cgt gat ttc ggc gcc tac ccg tcc gcg 1116
Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala
260 265 270
cac tcc gcc ttc atg ctc aac cac tcc ctc gag agc ctc gac gtg cgc 1164
His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg
275 280 285
atg cag cgc cac tgc gag aac gca cag agg gtc gcc gag tac ctc gag 1212
Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu
290 295 300
ggc cgc aag gac gtg ctt gtc gac gtc cgc tac ccc gga ctc gac tcc 1260
Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser
305 310 315 320
tcc ccg tac cgc gag ctg gcg aag aag tac ctc agc ggc aac tcc ggc 1308
Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly
325 330 335
gtg ctg tgc atc gac atc aag ggg ggc cgc gag gcc ggc gtg aag ttc 1356
Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe
340 345 350
atg gac gcc ctc gag ctg gtc acc cgc cag gtg cac gtc gcc gac gcc 1404
Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala
355 360 365
cgt tcc tgc gtg ctg cac ccg gcg tcg acc acg cac cgc cag gtg ccg 1452
Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro
370 375 380
gac gag cag ctg ccg tcc gtc ggc atc acc ccg ggt ctg gtg cgc atc 1500
Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile
385 390 395 400
tcc gtc ggc ctg gag aac gtc gac gac atc atc gcc gac atc gag cag 1548
Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln
405 410 415
gcc ctg gag aag tcc cag gcc taa gttcgcttat cgacgccgcc ccggtccctc 1602
Ala Leu Glu Lys Ser Gln Ala
420
atgtgggacc ggggcggtgg ctttcggggg atggctgcac tttcaccccg actgggtgcg 1662
tgtcgacaac ggggtttggg ggacagcgat gagggtataa gtgcagacac ggagaaggcc 1722
cggacactgt gggaggtgtc cgggccttgg gggcgtcgat aagcggtgtc tctagaactc 1782
cggcagcggc agaacagtga gggagaacat cgcgacgatg gccatgacca gcgggatggc 1842
gatcatgatc ggggcggcga tagccgggtt 1872
<210> 62
<211> 423
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 62
Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn
1 5 10 15
Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe
20 25 30
Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala
35 40 45
Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr
50 55 60
Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser
65 70 75 80
Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly
85 90 95
Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu
100 105 110
Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp
115 120 125
Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr
130 135 140
Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu
145 150 155 160
Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu
165 170 175
Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu
180 185 190
His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly
195 200 205
His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp
210 215 220
Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr
225 230 235 240
His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile
245 250 255
Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala
260 265 270
His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg
275 280 285
Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu
290 295 300
Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser
305 310 315 320
Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly
325 330 335
Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe
340 345 350
Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala
355 360 365
Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro
370 375 380
Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile
385 390 395 400
Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln
405 410 415
Ala Leu Glu Lys Ser Gln Ala
420
<210> 63
<211> 4014
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(3714)
<223> ген pyc
<400> 63
cggcaccgga ccaccgcgac gcccacggca ctgcccacac attggggtgg tgcgcgtggg 60
tttcgctgtt cggaacacct gtcggcccat tccgcagggc gggacagaaa cggcctttcg 120
ggcgcccgtc ccgcggagtg cggaaatgga ccgcaccggg tccaccacct gcagataccc 180
cctgtgacta ggggatactc ccccgaagac aaccctcttt cttgcgatac tgtggtgtgc 240
cagagagctc acccgctctc agacacaccc ccgacatgac gttcggaaag gaaccccacc 300
gtg gcc aca acc act ctg cca gcc ttc aca aag gtg ctg gtc gcc aac 348
Val Ala Thr Thr Thr Leu Pro Ala Phe Thr Lys Val Leu Val Ala Asn
1 5 10 15
cgc ggc gag atc gcc gtc cgc gcc ttc cgt gcc gcc ttc gag acc ggt 396
Arg Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala Phe Glu Thr Gly
20 25 30
gcc tcc acc gtc gcg gtc tac ccg aag gag gac cgc aac tcg cac cac 444
Ala Ser Thr Val Ala Val Tyr Pro Lys Glu Asp Arg Asn Ser His His
35 40 45
cgc tcc ttc gcc tcc gag gcc gtg cgc atc ggc acc gag gga tcg ccg 492
Arg Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro
50 55 60
gtc aag gcg tac ctc gac atc gac gag atc atc cgc gcc gcc aag aag 540
Val Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Arg Ala Ala Lys Lys
65 70 75 80
acc cac gcc gac gcc gtc tac ccc ggc tac ggt ttc ctc tcc gag aac 588
Thr His Ala Asp Ala Val Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn
85 90 95
gcc cag ctc gcc cgc gag tgc gcc gag aac ggc ctc acc ttc atc ggc 636
Ala Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Leu Thr Phe Ile Gly
100 105 110
ccg ccg ccg gag gtc ctc gac ctg acc ggc gac aag tcc gcc gcc gtc 684
Pro Pro Pro Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Ala Ala Val
115 120 125
gag gcc gcc cgg aag gcc gga ctc ccg gtc ctg cag gac tcc gag ccg 732
Glu Ala Ala Arg Lys Ala Gly Leu Pro Val Leu Gln Asp Ser Glu Pro
130 135 140
tcc acc gac atc gac gag ctc gtc gag atg tcg aag gac ttc acc ttc 780
Ser Thr Asp Ile Asp Glu Leu Val Glu Met Ser Lys Asp Phe Thr Phe
145 150 155 160
ccg atc ttc gtc aag gcc gtc gcc ggt ggc ggt gga cgt ggc atg cgc 828
Pro Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg
165 170 175
ttc atc gag aag ccc gag gac gtc gcc cag ctg gcc acc gag gcc tcc 876
Phe Ile Glu Lys Pro Glu Asp Val Ala Gln Leu Ala Thr Glu Ala Ser
180 185 190
cgc gag gcg gag gcc gcc ttc ggt gac ggt cat gtc tac ctc gag cgt 924
Arg Glu Ala Glu Ala Ala Phe Gly Asp Gly His Val Tyr Leu Glu Arg
195 200 205
gcg gtg ctc aac ccg cag cac atc gag gtc cag atc ctc gcc gac cac 972
Ala Val Leu Asn Pro Gln His Ile Glu Val Gln Ile Leu Ala Asp His
210 215 220
acc ggc gac gtc atc cac ctc tac gag cgt gac tgc tcc ctg cag cgc 1020
Thr Gly Asp Val Ile His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg
225 230 235 240
cgc cac cag aag gtc gtg gag atc gcc ccg gcc cag cac ctc gac cca 1068
Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro
245 250 255
gcc ctg cgc gac cag atc tgc gcc gac gcc gtg aag ttc tgc cgc tcc 1116
Ala Leu Arg Asp Gln Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser
260 265 270
atc ggc tac gtg tgc gcc ggc acc gtc gag ttc ctc gtc gac gag aac 1164
Ile Gly Tyr Val Cys Ala Gly Thr Val Glu Phe Leu Val Asp Glu Asn
275 280 285
ggc aac cac gtc ttc atc gag atg aac ccg cgc atc cag gtc gag cac 1212
Gly Asn His Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His
290 295 300
acc atc acc gag gag gtc acc tcc gtc gac ctg gtc aag gcg cag atg 1260
Thr Ile Thr Glu Glu Val Thr Ser Val Asp Leu Val Lys Ala Gln Met
305 310 315 320
aac atc gcc gcc ggc gcg acc ctc aag gac ctc ggc ctc acg cag gag 1308
Asn Ile Ala Ala Gly Ala Thr Leu Lys Asp Leu Gly Leu Thr Gln Glu
325 330 335
tcc atc aag ctc cac ggc gcg gcc ctg cag tgc cgc atc acc acc gag 1356
Ser Ile Lys Leu His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu
340 345 350
gac ccg gcc aac aac ttc cgc ccg gac acc ggc acc atc acc gcg tac 1404
Asp Pro Ala Asn Asn Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr
355 360 365
cgc tcc ccg ggt ggc gcg ggc gtg cgt ctc gac ggc gcc gcg tcc ctc 1452
Arg Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Ser Leu
370 375 380
ggc ggc gag atc tcc ccg aac ttc gac tcc ctg ctg gtc aag atg acc 1500
Gly Gly Glu Ile Ser Pro Asn Phe Asp Ser Leu Leu Val Lys Met Thr
385 390 395 400
tgc cgc ggc gcc gac ttc gcc acc gcc gtc gcc cgt gcc cag cgc gcg 1548
Cys Arg Gly Ala Asp Phe Ala Thr Ala Val Ala Arg Ala Gln Arg Ala
405 410 415
ctc aac gag ttc acc atc tcg ggc gtg gcc acc aac atc ggc ttc ctc 1596
Leu Asn Glu Phe Thr Ile Ser Gly Val Ala Thr Asn Ile Gly Phe Leu
420 425 430
cgc gcc ctg ctg cgc gag gag gac ttc cag acc cgc cgc atc tcc acc 1644
Arg Ala Leu Leu Arg Glu Glu Asp Phe Gln Thr Arg Arg Ile Ser Thr
435 440 445
aac ttc atc ggc gag cac ctc cac ctg ctg tcc gcc ccg ccg gcc gac 1692
Asn Phe Ile Gly Glu His Leu His Leu Leu Ser Ala Pro Pro Ala Asp
450 455 460
gac gag gcc ggc cgc atc ctc aac tac ctc gcg gac gtc acc gtc aac 1740
Asp Glu Ala Gly Arg Ile Leu Asn Tyr Leu Ala Asp Val Thr Val Asn
465 470 475 480
cgt ccg cac ggc aag cgc ccc acc gag atc cgt ccg gtg acc aag ctg 1788
Arg Pro His Gly Lys Arg Pro Thr Glu Ile Arg Pro Val Thr Lys Leu
485 490 495
ccg gcc cgc gac gac agc ccg ctg ccg cgc ggt tcc cgc gac gtc ctg 1836
Pro Ala Arg Asp Asp Ser Pro Leu Pro Arg Gly Ser Arg Asp Val Leu
500 505 510
cgt gac ctg ggc ccg cag aag ttc gcc gag cag ctg cgc aac cag gac 1884
Arg Asp Leu Gly Pro Gln Lys Phe Ala Glu Gln Leu Arg Asn Gln Asp
515 520 525
gcc ctg gcc gtc acc gac acc acg ttc cgt gac gcg cac cag tcg ctg 1932
Ala Leu Ala Val Thr Asp Thr Thr Phe Arg Asp Ala His Gln Ser Leu
530 535 540
ctg gcc acc cgc atc cgt tcc tcc gcg ctc gtc gcc gcc gcc gag cac 1980
Leu Ala Thr Arg Ile Arg Ser Ser Ala Leu Val Ala Ala Ala Glu His
545 550 555 560
gtc ggc cgc ctg acc ccg gag ctg ctc tcc gtc gag gcg tgg ggc ggt 2028
Val Gly Arg Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly
565 570 575
gcg acc tac gac gtc gcg atg cgt ttc ctc cac gag gac ccg tgg gag 2076
Ala Thr Tyr Asp Val Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu
580 585 590
cgt ctc gac gac ctg cgc cgc gcc atg ccg aac gtc aac atc cag atg 2124
Arg Leu Asp Asp Leu Arg Arg Ala Met Pro Asn Val Asn Ile Gln Met
595 600 605
ctg ctg cgc ggc cgc aac acc gtc ggc tac acc tcc tac ccg gac agc 2172
Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Ser Tyr Pro Asp Ser
610 615 620
gtc ggc cag gcc ttc gtc aag gag gcc gcc gac tcc ggc gtc gac atc 2220
Val Gly Gln Ala Phe Val Lys Glu Ala Ala Asp Ser Gly Val Asp Ile
625 630 635 640
ttc cgc atc ttc gac gcc ctc aac gac gtg gac cag atg cgc ccg gcc 2268
Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Asp Gln Met Arg Pro Ala
645 650 655
atc gat gcc gtc ctg gag acc ggc acc acc gtc gcc gag gtc gcc atg 2316
Ile Asp Ala Val Leu Glu Thr Gly Thr Thr Val Ala Glu Val Ala Met
660 665 670
gcc tac tcc ggc aac ctg ctg gac ccg aac gag aag ctc tac acg ctc 2364
Ala Tyr Ser Gly Asn Leu Leu Asp Pro Asn Glu Lys Leu Tyr Thr Leu
675 680 685
gac tac tac ctc cgt ctg gcc gag gag atc gtc gag tcc ggt gcc cac 2412
Asp Tyr Tyr Leu Arg Leu Ala Glu Glu Ile Val Glu Ser Gly Ala His
690 695 700
atc ctg gcc atc aag gac atg gcc ggt ctg ctc cgc ccg acc gcg gcc 2460
Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Thr Ala Ala
705 710 715 720
tcc cgc ctg gtg aag gcg ctg cgc agc aac ttc gac ctg ccg gtg cac 2508
Ser Arg Leu Val Lys Ala Leu Arg Ser Asn Phe Asp Leu Pro Val His
725 730 735
gtg cac acc cac gac acc gcc ggc ggt cag ctg gcg acc tac ctg gcc 2556
Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Leu Ala
740 745 750
gcc gcg cag gcg ggt gcc gac gcc gtc gac ggc gcc tcc gcc ccg ctg 2604
Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Ala Pro Leu
755 760 765
gcc ggc acg acc tcg cag ccc tcg ctg tcc gcg atc gtc gcg gcc ttc 2652
Ala Gly Thr Thr Ser Gln Pro Ser Leu Ser Ala Ile Val Ala Ala Phe
770 775 780
gcc gac acc cac cgc gac acc ggc ctg tcc ctg cag gcc gtg tcc gac 2700
Ala Asp Thr His Arg Asp Thr Gly Leu Ser Leu Gln Ala Val Ser Asp
785 790 795 800
ctc gag ccc tac tgg gag gcc gtg cgc cag ctc tac gcc ccc ttc gag 2748
Leu Glu Pro Tyr Trp Glu Ala Val Arg Gln Leu Tyr Ala Pro Phe Glu
805 810 815
gcg ggc gtg ccc ggc ccg acg ggc cgc gtg tac aag cac gag atc ccg 2796
Ala Gly Val Pro Gly Pro Thr Gly Arg Val Tyr Lys His Glu Ile Pro
820 825 830
ggt ggt cag ctg tcg aac ctg cgc acg cag gcc aag gcc ctg ggt ctg 2844
Gly Gly Gln Leu Ser Asn Leu Arg Thr Gln Ala Lys Ala Leu Gly Leu
835 840 845
gag gac cgc ttc gag ctc atc gag gac tac tac gcc gcc gtc aac gag 2892
Glu Asp Arg Phe Glu Leu Ile Glu Asp Tyr Tyr Ala Ala Val Asn Glu
850 855 860
atg ctg ggc cgc ccc acc aag gtc acc ccg tcc tcc aag gtc gtc ggc 2940
Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly
865 870 875 880
gac ctg gcg ctc cac ctc gtc ggc gcg ggc gtc gac ccg cac gac ttc 2988
Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro His Asp Phe
885 890 895
gcc aat gac ccg cag aag tac gac atc ccg gac tcc gtg atc gac ttc 3036
Ala Asn Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Asp Phe
900 905 910
ctc cgc ggt gac ctc ggc aac ccg ccc ggt ggc tgg ccg atg ctg cgg 3084
Leu Arg Gly Asp Leu Gly Asn Pro Pro Gly Gly Trp Pro Met Leu Arg
915 920 925
gag aag gcc ctg gca ggt cgt tcc tcc acg cag tcc aag ctt gtc gac 3132
Glu Lys Ala Leu Ala Gly Arg Ser Ser Thr Gln Ser Lys Leu Val Asp
930 935 940
gtc gcc ccg gag gac gag gag gcc ctg cag agc gcg gac cgc cag acc 3180
Val Ala Pro Glu Asp Glu Glu Ala Leu Gln Ser Ala Asp Arg Gln Thr
945 950 955 960
cgc cgc tcg acc ctg gac agg ctg ctg ttc ccg aag cag gcc gcc gag 3228
Arg Arg Ser Thr Leu Asp Arg Leu Leu Phe Pro Lys Gln Ala Ala Glu
965 970 975
ttc gcg gag cac cgc cgc cag tac ggc aac acc gcc gcc ctc gag gac 3276
Phe Ala Glu His Arg Arg Gln Tyr Gly Asn Thr Ala Ala Leu Glu Asp
980 985 990
cgc gtg ttc ttc tac ggc ctc aag gag ggc gag gag aac gtc atc cgc 3324
Arg Val Phe Phe Tyr Gly Leu Lys Glu Gly Glu Glu Asn Val Ile Arg
995 1000 1005
ctc atc ggg gag cac ggt cag ccg ccg atg gtc gtg cgt ctc gac 3369
Leu Ile Gly Glu His Gly Gln Pro Pro Met Val Val Arg Leu Asp
1010 1015 1020
gcc gtc ggt gag ccc gac gag aag ggc atg cgc cag gtg gtg acc 3414
Ala Val Gly Glu Pro Asp Glu Lys Gly Met Arg Gln Val Val Thr
1025 1030 1035
aac gtc aac ggc cag atc cgt ccg atg aag gtc cgt gac cgc tcc 3459
Asn Val Asn Gly Gln Ile Arg Pro Met Lys Val Arg Asp Arg Ser
1040 1045 1050
gtc gag tcg gtc acc gcc tcc gcg gag aag gcc gac acc acc aac 3504
Val Glu Ser Val Thr Ala Ser Ala Glu Lys Ala Asp Thr Thr Asn
1055 1060 1065
ccg ggc cac gtc gcc gcg ccg ttc gcg ggt gtc gtc atg gtc acc 3549
Pro Gly His Val Ala Ala Pro Phe Ala Gly Val Val Met Val Thr
1070 1075 1080
gcg aag gtg ggc gac gag gtc aag gcc ggc gac ccg gta gcc gtg 3594
Ala Lys Val Gly Asp Glu Val Lys Ala Gly Asp Pro Val Ala Val
1085 1090 1095
atc gag gcc atg aag atg gag gcc acc atc acc gcc tcc aag gac 3639
Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala Ser Lys Asp
1100 1105 1110
ggc gtg gtc gac cgc gtc gcc ctg gcc cag gcc acg aag gtg gag 3684
Gly Val Val Asp Arg Val Ala Leu Ala Gln Ala Thr Lys Val Glu
1115 1120 1125
ggc ggc gac ctc atc gtc gtc atc gcc tag ccgcacaacg cgaaagcccc 3734
Gly Gly Asp Leu Ile Val Val Ile Ala
1130 1135
gccactcctt ccgggagtgg cggggcttct ctgtgcgggt gggccgctac ttgatctcga 3794
gcaggacctg gcccttggtc acgccggcac cggcctcgac ggacaggccc tcgaccttgc 3854
ccgacttgtg ggccttgacc gggttctcca tcttcatggc ctcgaggacc acgacgacgt 3914
cgccctcggc gacctcctgg ccgtcctcga cgttgacctt gatgacggtg ccctgcatcg 3974
gggccgccac agcgtcaccg gaggcacctg cggcaccgcc 4014
<210> 64
<211> 1137
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 64
Val Ala Thr Thr Thr Leu Pro Ala Phe Thr Lys Val Leu Val Ala Asn
1 5 10 15
Arg Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala Phe Glu Thr Gly
20 25 30
Ala Ser Thr Val Ala Val Tyr Pro Lys Glu Asp Arg Asn Ser His His
35 40 45
Arg Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro
50 55 60
Val Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Arg Ala Ala Lys Lys
65 70 75 80
Thr His Ala Asp Ala Val Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn
85 90 95
Ala Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Leu Thr Phe Ile Gly
100 105 110
Pro Pro Pro Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Ala Ala Val
115 120 125
Glu Ala Ala Arg Lys Ala Gly Leu Pro Val Leu Gln Asp Ser Glu Pro
130 135 140
Ser Thr Asp Ile Asp Glu Leu Val Glu Met Ser Lys Asp Phe Thr Phe
145 150 155 160
Pro Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg
165 170 175
Phe Ile Glu Lys Pro Glu Asp Val Ala Gln Leu Ala Thr Glu Ala Ser
180 185 190
Arg Glu Ala Glu Ala Ala Phe Gly Asp Gly His Val Tyr Leu Glu Arg
195 200 205
Ala Val Leu Asn Pro Gln His Ile Glu Val Gln Ile Leu Ala Asp His
210 215 220
Thr Gly Asp Val Ile His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg
225 230 235 240
Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro
245 250 255
Ala Leu Arg Asp Gln Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser
260 265 270
Ile Gly Tyr Val Cys Ala Gly Thr Val Glu Phe Leu Val Asp Glu Asn
275 280 285
Gly Asn His Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His
290 295 300
Thr Ile Thr Glu Glu Val Thr Ser Val Asp Leu Val Lys Ala Gln Met
305 310 315 320
Asn Ile Ala Ala Gly Ala Thr Leu Lys Asp Leu Gly Leu Thr Gln Glu
325 330 335
Ser Ile Lys Leu His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu
340 345 350
Asp Pro Ala Asn Asn Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr
355 360 365
Arg Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Ser Leu
370 375 380
Gly Gly Glu Ile Ser Pro Asn Phe Asp Ser Leu Leu Val Lys Met Thr
385 390 395 400
Cys Arg Gly Ala Asp Phe Ala Thr Ala Val Ala Arg Ala Gln Arg Ala
405 410 415
Leu Asn Glu Phe Thr Ile Ser Gly Val Ala Thr Asn Ile Gly Phe Leu
420 425 430
Arg Ala Leu Leu Arg Glu Glu Asp Phe Gln Thr Arg Arg Ile Ser Thr
435 440 445
Asn Phe Ile Gly Glu His Leu His Leu Leu Ser Ala Pro Pro Ala Asp
450 455 460
Asp Glu Ala Gly Arg Ile Leu Asn Tyr Leu Ala Asp Val Thr Val Asn
465 470 475 480
Arg Pro His Gly Lys Arg Pro Thr Glu Ile Arg Pro Val Thr Lys Leu
485 490 495
Pro Ala Arg Asp Asp Ser Pro Leu Pro Arg Gly Ser Arg Asp Val Leu
500 505 510
Arg Asp Leu Gly Pro Gln Lys Phe Ala Glu Gln Leu Arg Asn Gln Asp
515 520 525
Ala Leu Ala Val Thr Asp Thr Thr Phe Arg Asp Ala His Gln Ser Leu
530 535 540
Leu Ala Thr Arg Ile Arg Ser Ser Ala Leu Val Ala Ala Ala Glu His
545 550 555 560
Val Gly Arg Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly
565 570 575
Ala Thr Tyr Asp Val Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu
580 585 590
Arg Leu Asp Asp Leu Arg Arg Ala Met Pro Asn Val Asn Ile Gln Met
595 600 605
Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Ser Tyr Pro Asp Ser
610 615 620
Val Gly Gln Ala Phe Val Lys Glu Ala Ala Asp Ser Gly Val Asp Ile
625 630 635 640
Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Asp Gln Met Arg Pro Ala
645 650 655
Ile Asp Ala Val Leu Glu Thr Gly Thr Thr Val Ala Glu Val Ala Met
660 665 670
Ala Tyr Ser Gly Asn Leu Leu Asp Pro Asn Glu Lys Leu Tyr Thr Leu
675 680 685
Asp Tyr Tyr Leu Arg Leu Ala Glu Glu Ile Val Glu Ser Gly Ala His
690 695 700
Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Thr Ala Ala
705 710 715 720
Ser Arg Leu Val Lys Ala Leu Arg Ser Asn Phe Asp Leu Pro Val His
725 730 735
Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Leu Ala
740 745 750
Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Ala Pro Leu
755 760 765
Ala Gly Thr Thr Ser Gln Pro Ser Leu Ser Ala Ile Val Ala Ala Phe
770 775 780
Ala Asp Thr His Arg Asp Thr Gly Leu Ser Leu Gln Ala Val Ser Asp
785 790 795 800
Leu Glu Pro Tyr Trp Glu Ala Val Arg Gln Leu Tyr Ala Pro Phe Glu
805 810 815
Ala Gly Val Pro Gly Pro Thr Gly Arg Val Tyr Lys His Glu Ile Pro
820 825 830
Gly Gly Gln Leu Ser Asn Leu Arg Thr Gln Ala Lys Ala Leu Gly Leu
835 840 845
Glu Asp Arg Phe Glu Leu Ile Glu Asp Tyr Tyr Ala Ala Val Asn Glu
850 855 860
Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly
865 870 875 880
Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro His Asp Phe
885 890 895
Ala Asn Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Asp Phe
900 905 910
Leu Arg Gly Asp Leu Gly Asn Pro Pro Gly Gly Trp Pro Met Leu Arg
915 920 925
Glu Lys Ala Leu Ala Gly Arg Ser Ser Thr Gln Ser Lys Leu Val Asp
930 935 940
Val Ala Pro Glu Asp Glu Glu Ala Leu Gln Ser Ala Asp Arg Gln Thr
945 950 955 960
Arg Arg Ser Thr Leu Asp Arg Leu Leu Phe Pro Lys Gln Ala Ala Glu
965 970 975
Phe Ala Glu His Arg Arg Gln Tyr Gly Asn Thr Ala Ala Leu Glu Asp
980 985 990
Arg Val Phe Phe Tyr Gly Leu Lys Glu Gly Glu Glu Asn Val Ile Arg
995 1000 1005
Leu Ile Gly Glu His Gly Gln Pro Pro Met Val Val Arg Leu Asp
1010 1015 1020
Ala Val Gly Glu Pro Asp Glu Lys Gly Met Arg Gln Val Val Thr
1025 1030 1035
Asn Val Asn Gly Gln Ile Arg Pro Met Lys Val Arg Asp Arg Ser
1040 1045 1050
Val Glu Ser Val Thr Ala Ser Ala Glu Lys Ala Asp Thr Thr Asn
1055 1060 1065
Pro Gly His Val Ala Ala Pro Phe Ala Gly Val Val Met Val Thr
1070 1075 1080
Ala Lys Val Gly Asp Glu Val Lys Ala Gly Asp Pro Val Ala Val
1085 1090 1095
Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala Ser Lys Asp
1100 1105 1110
Gly Val Val Asp Arg Val Ala Leu Ala Gln Ala Thr Lys Val Glu
1115 1120 1125
Gly Gly Asp Leu Ile Val Val Ile Ala
1130 1135
<210> 65
<211> 2190
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1890)
<223> ген serA
<400> 65
cgacttcgcc aacttcctcc gcgaccgccg ttcccgctac gtcggcatgg tcgagtacgc 60
gatggacaag cccgtggtcc gcgacgtcga cgacagtgac tacacggggg gcgaggaggg 120
cccgaacgcg ttcactgact gaacgggggt gaccggtgga gtggtgtgcc tcatatcggg 180
gcagggtatg ggacaaggcg caaccccaca gactaaagtg ttatccgttc ctcgttcctg 240
gttcggtcgg gtgcgctgac cggccccggg gcctcatttt caaatcagga gatatcacac 300
gtg agc aag ccc gtc gtc ctc atc gcc gac aaa ctg gca cct tcc acc 348
Val Ser Lys Pro Val Val Leu Ile Ala Asp Lys Leu Ala Pro Ser Thr
1 5 10 15
gta gat gcg ctc gga gac gcc gtg gag gtg cgc tgg gtc gac ggc ccg 396
Val Asp Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val Asp Gly Pro
20 25 30
aac acc ccg gag ctg ctc gcc gcc gtc ccg gag gca gac gca ctg ctg 444
Asn Thr Pro Glu Leu Leu Ala Ala Val Pro Glu Ala Asp Ala Leu Leu
35 40 45
gtc cgt tcc gcg acc acc gtc acc cgt gag gtc ctc gag gcc gca ccg 492
Val Arg Ser Ala Thr Thr Val Thr Arg Glu Val Leu Glu Ala Ala Pro
50 55 60
aac ctg aag atc gtc ggc cgc gcc ggc gtc ggc ctg gac aac gtc gac 540
Asn Leu Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp Asn Val Asp
65 70 75 80
atc gac gcc gcc acc gag cgt ggc gtc atg gtc gtc aac gcc ccg acc 588
Ile Asp Ala Ala Thr Glu Arg Gly Val Met Val Val Asn Ala Pro Thr
85 90 95
tcc aac atc cac tcc gcc tgc gag cac gcc atc gcc ctg ctc atg gcc 636
Ser Asn Ile His Ser Ala Cys Glu His Ala Ile Ala Leu Leu Met Ala
100 105 110
acc gtg cgc cag atc ccg gcc gcc gac gcc acc ctg cgt gag ggc gag 684
Thr Val Arg Gln Ile Pro Ala Ala Asp Ala Thr Leu Arg Glu Gly Glu
115 120 125
tgg aag cgt tcc gcc ttc aag ggc gtg gag atc ttc ggc aag acc atc 732
Trp Lys Arg Ser Ala Phe Lys Gly Val Glu Ile Phe Gly Lys Thr Ile
130 135 140
ggc atc gtc ggc ttc ggc cac atc ggc cag ctc ttc gcc cag cgt ctg 780
Gly Ile Val Gly Phe Gly His Ile Gly Gln Leu Phe Ala Gln Arg Leu
145 150 155 160
tcc gcc ttc gag acc gag atc atc gcc tac gac ccg tac gcg aac ccg 828
Ser Ala Phe Glu Thr Glu Ile Ile Ala Tyr Asp Pro Tyr Ala Asn Pro
165 170 175
acc cgc gcc gcc cag ctc ggc gtc gag ctg gtc gag ctg gag gag ctg 876
Thr Arg Ala Ala Gln Leu Gly Val Glu Leu Val Glu Leu Glu Glu Leu
180 185 190
gtc tcc cgc gcc gac atc gtc acc atc cac ctt ccg aag acc aag gag 924
Val Ser Arg Ala Asp Ile Val Thr Ile His Leu Pro Lys Thr Lys Glu
195 200 205
acg gcc ggc atg ttc aac gcc gag ctg ctc gcc aag tcc aag aag ggc 972
Thr Ala Gly Met Phe Asn Ala Glu Leu Leu Ala Lys Ser Lys Lys Gly
210 215 220
cag atc atc atc aac gcc gcc cgc ggt ggc ctc gtc gat gag cag gca 1020
Gln Ile Ile Ile Asn Ala Ala Arg Gly Gly Leu Val Asp Glu Gln Ala
225 230 235 240
ctg gct gac gcc atc aag tcc ggc cac atc tgg ggc gcc ggc ttc gac 1068
Leu Ala Asp Ala Ile Lys Ser Gly His Ile Trp Gly Ala Gly Phe Asp
245 250 255
gtc ttc tcc acc gag ccg tgc acc gac tcc ccg ctg ttc ggc ctg ccg 1116
Val Phe Ser Thr Glu Pro Cys Thr Asp Ser Pro Leu Phe Gly Leu Pro
260 265 270
gag gtc gtc gtg acc ccg cac ctg ggt gcc tcc acc gaa gag gcc cag 1164
Glu Val Val Val Thr Pro His Leu Gly Ala Ser Thr Glu Glu Ala Gln
275 280 285
gac cgt gcc ggc acc gac gtc gcc gcc tcc gtc ctc aag gcc ctg gcc 1212
Asp Arg Ala Gly Thr Asp Val Ala Ala Ser Val Leu Lys Ala Leu Ala
290 295 300
ggc gag ttc gtc ccg gac gcc gtg aac gtc tcc ggc ggc cgc gtc ggc 1260
Gly Glu Phe Val Pro Asp Ala Val Asn Val Ser Gly Gly Arg Val Gly
305 310 315 320
gag gag gtc gcc ctg tgg ttg gac ctg acc cgc aag ctg ggt ctg ctg 1308
Glu Glu Val Ala Leu Trp Leu Asp Leu Thr Arg Lys Leu Gly Leu Leu
325 330 335
gcc ggt cag ctg ctg gac tcc gcc ccg gtc gac ctc gag gtc gag gcc 1356
Ala Gly Gln Leu Leu Asp Ser Ala Pro Val Asp Leu Glu Val Glu Ala
340 345 350
cgc ggc gag ctc tcc acc gag gac gac ctg tcc gtg ctc ggc ctg tcc 1404
Arg Gly Glu Leu Ser Thr Glu Asp Asp Leu Ser Val Leu Gly Leu Ser
355 360 365
gcc gtc cgt ggc ctg ttc tcc gcc acc acc gac gag ccg gtc acc ttc 1452
Ala Val Arg Gly Leu Phe Ser Ala Thr Thr Asp Glu Pro Val Thr Phe
370 375 380
gtc aac gcc ccg cag atc gcg gag agc cgt ggc ctg gac tac aag gtc 1500
Val Asn Ala Pro Gln Ile Ala Glu Ser Arg Gly Leu Asp Tyr Lys Val
385 390 395 400
gac acc gcg acc gag tcc acc acc cac cgt tcc tcc gtc gag gtc aag 1548
Asp Thr Ala Thr Glu Ser Thr Thr His Arg Ser Ser Val Glu Val Lys
405 410 415
gtc atc ggt gcg gac ggc tcc tcc cgc tcc gtc gtc ggt gcc ctg acc 1596
Val Ile Gly Ala Asp Gly Ser Ser Arg Ser Val Val Gly Ala Leu Thr
420 425 430
ggc ctg gag cgc gtc gag aag atc gtg cgc atc gac gag cgc ggc ctg 1644
Gly Leu Glu Arg Val Glu Lys Ile Val Arg Ile Asp Glu Arg Gly Leu
435 440 445
gac atg cgc gcc gag ggt cgc aac ctc ttc ctc gag tac acc gac gcc 1692
Asp Met Arg Ala Glu Gly Arg Asn Leu Phe Leu Glu Tyr Thr Asp Ala
450 455 460
ccg ggc gcc ctg ggc aag gtg ggc tcc cag ctg ggc gac gcc ggc atc 1740
Pro Gly Ala Leu Gly Lys Val Gly Ser Gln Leu Gly Asp Ala Gly Ile
465 470 475 480
aac atc gag gcc gcc gcc ctc acc cag gcc acc aag ggt gac ggc gcc 1788
Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Thr Lys Gly Asp Gly Ala
485 490 495
atc ctg atc ctg cgt gtc gac gca gag atc ccg gag gca ctg cag aag 1836
Ile Leu Ile Leu Arg Val Asp Ala Glu Ile Pro Glu Ala Leu Gln Lys
500 505 510
cag atc gcc gag tcc ctg ggt gcc acc aac gat atc cag ctc aac ctg 1884
Gln Ile Ala Glu Ser Leu Gly Ala Thr Asn Asp Ile Gln Leu Asn Leu
515 520 525
gac tag tctccggtcg ccccgtcgac ggcgtgtcgc gtcggtccct cgcggatcgc 1940
Asp
gcggcacgcc gttcgcgttt ctgcgggagg cagtttcatt catgccgcct gaccgggaaa 2000
tgtgcaggta gacagtatgg ttcattccag gcgcgccgtt aatagaccga acggtatgga 2060
ctaactagac ggggaagtgt tgaatgggtc aggcaacgaa ccgactgatc caggagtacc 2120
ccgtgtccga cctcaccccg aaccacaccg ccggcgaacc gctcgagccc atcgacgcgc 2180
agaagctcgc 2190
<210> 66
<211> 529
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 66
Val Ser Lys Pro Val Val Leu Ile Ala Asp Lys Leu Ala Pro Ser Thr
1 5 10 15
Val Asp Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val Asp Gly Pro
20 25 30
Asn Thr Pro Glu Leu Leu Ala Ala Val Pro Glu Ala Asp Ala Leu Leu
35 40 45
Val Arg Ser Ala Thr Thr Val Thr Arg Glu Val Leu Glu Ala Ala Pro
50 55 60
Asn Leu Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp Asn Val Asp
65 70 75 80
Ile Asp Ala Ala Thr Glu Arg Gly Val Met Val Val Asn Ala Pro Thr
85 90 95
Ser Asn Ile His Ser Ala Cys Glu His Ala Ile Ala Leu Leu Met Ala
100 105 110
Thr Val Arg Gln Ile Pro Ala Ala Asp Ala Thr Leu Arg Glu Gly Glu
115 120 125
Trp Lys Arg Ser Ala Phe Lys Gly Val Glu Ile Phe Gly Lys Thr Ile
130 135 140
Gly Ile Val Gly Phe Gly His Ile Gly Gln Leu Phe Ala Gln Arg Leu
145 150 155 160
Ser Ala Phe Glu Thr Glu Ile Ile Ala Tyr Asp Pro Tyr Ala Asn Pro
165 170 175
Thr Arg Ala Ala Gln Leu Gly Val Glu Leu Val Glu Leu Glu Glu Leu
180 185 190
Val Ser Arg Ala Asp Ile Val Thr Ile His Leu Pro Lys Thr Lys Glu
195 200 205
Thr Ala Gly Met Phe Asn Ala Glu Leu Leu Ala Lys Ser Lys Lys Gly
210 215 220
Gln Ile Ile Ile Asn Ala Ala Arg Gly Gly Leu Val Asp Glu Gln Ala
225 230 235 240
Leu Ala Asp Ala Ile Lys Ser Gly His Ile Trp Gly Ala Gly Phe Asp
245 250 255
Val Phe Ser Thr Glu Pro Cys Thr Asp Ser Pro Leu Phe Gly Leu Pro
260 265 270
Glu Val Val Val Thr Pro His Leu Gly Ala Ser Thr Glu Glu Ala Gln
275 280 285
Asp Arg Ala Gly Thr Asp Val Ala Ala Ser Val Leu Lys Ala Leu Ala
290 295 300
Gly Glu Phe Val Pro Asp Ala Val Asn Val Ser Gly Gly Arg Val Gly
305 310 315 320
Glu Glu Val Ala Leu Trp Leu Asp Leu Thr Arg Lys Leu Gly Leu Leu
325 330 335
Ala Gly Gln Leu Leu Asp Ser Ala Pro Val Asp Leu Glu Val Glu Ala
340 345 350
Arg Gly Glu Leu Ser Thr Glu Asp Asp Leu Ser Val Leu Gly Leu Ser
355 360 365
Ala Val Arg Gly Leu Phe Ser Ala Thr Thr Asp Glu Pro Val Thr Phe
370 375 380
Val Asn Ala Pro Gln Ile Ala Glu Ser Arg Gly Leu Asp Tyr Lys Val
385 390 395 400
Asp Thr Ala Thr Glu Ser Thr Thr His Arg Ser Ser Val Glu Val Lys
405 410 415
Val Ile Gly Ala Asp Gly Ser Ser Arg Ser Val Val Gly Ala Leu Thr
420 425 430
Gly Leu Glu Arg Val Glu Lys Ile Val Arg Ile Asp Glu Arg Gly Leu
435 440 445
Asp Met Arg Ala Glu Gly Arg Asn Leu Phe Leu Glu Tyr Thr Asp Ala
450 455 460
Pro Gly Ala Leu Gly Lys Val Gly Ser Gln Leu Gly Asp Ala Gly Ile
465 470 475 480
Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Thr Lys Gly Asp Gly Ala
485 490 495
Ile Leu Ile Leu Arg Val Asp Ala Glu Ile Pro Glu Ala Leu Gln Lys
500 505 510
Gln Ile Ala Glu Ser Leu Gly Ala Thr Asn Asp Ile Gln Leu Asn Leu
515 520 525
Asp
<210> 67
<211> 1848
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1548)
<223> ген serB
<400> 67
cgtatccgct ccgagcgccc ggcgttcgag ctgcactacc cgcacatggt tgagcgcatg 60
cgtcgcgagg cacacaccgg tgcccacgac gagcgcgcgg agatcaccca gactccggct 120
ccggccgagg tccgctaaaa gggtttgaca tagacccctc cgactgacac gacccccggt 180
agatccctct accgggggtt gcggcatgtt cgaaaccccc tcccatgaaa gaatgatccc 240
gtggcagatc aagctcagaa ccccgatacc gccgacaccg aagcacagca gcagctgcag 300
gtg gcg ctc cag ccc gga ctg gag tcc gca gtc atc acc ctc acc ggc 348
Val Ala Leu Gln Pro Gly Leu Glu Ser Ala Val Ile Thr Leu Thr Gly
1 5 10 15
cgt gac cgg ccg ggc gtg acg gcc gcc ttc ttc cgc gtc ctg tcc gct 396
Arg Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala
20 25 30
cac gac gtc cag ctt ctc gac gtc gag cag tcc cag ttc cgt gga tac 444
His Asp Val Gln Leu Leu Asp Val Glu Gln Ser Gln Phe Arg Gly Tyr
35 40 45
ctc tcc ctc gcc gcc tac gtg ggc gtc gac ccg gag cgc gtc gac cgt 492
Leu Ser Leu Ala Ala Tyr Val Gly Val Asp Pro Glu Arg Val Asp Arg
50 55 60
ctc cgc gag ggc ctg acc gag acc ctc aag ggc cac ggg cag ctc gcc 540
Leu Arg Glu Gly Leu Thr Glu Thr Leu Lys Gly His Gly Gln Leu Ala
65 70 75 80
acc gtc gag atg gtc ccg gag ggc cag cac tcc agc ccg cgg tcc acg 588
Thr Val Glu Met Val Pro Glu Gly Gln His Ser Ser Pro Arg Ser Thr
85 90 95
cac gtg gcc gtc gtc ctg ggc aac ccg gtg acc gcc acc gac gtg tcg 636
His Val Ala Val Val Leu Gly Asn Pro Val Thr Ala Thr Asp Val Ser
100 105 110
gcc atc ggt cag acg ctg gcc aac tac ggc gcg aac atc gac cgt atc 684
Ala Ile Gly Gln Thr Leu Ala Asn Tyr Gly Ala Asn Ile Asp Arg Ile
115 120 125
cgc ggc atc gcg gac tac ccg atc acc ggc ctg gag atc atg atc acg 732
Arg Gly Ile Ala Asp Tyr Pro Ile Thr Gly Leu Glu Ile Met Ile Thr
130 135 140
atc gcc aac ccg gag ccg ggc ggc ggc aag gcc atg cgt aag gca ctg 780
Ile Ala Asn Pro Glu Pro Gly Gly Gly Lys Ala Met Arg Lys Ala Leu
145 150 155 160
gcg gag ctg acc acc gag ctg ggc gtc gac atc gcc atc gag cgt gcc 828
Ala Glu Leu Thr Thr Glu Leu Gly Val Asp Ile Ala Ile Glu Arg Ala
165 170 175
ggc ctg ctg cgc cgc tcc aag cgt ctg gtc atc ttc gac tgc gac tcc 876
Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Ile Phe Asp Cys Asp Ser
180 185 190
acc ctc atc acc ggt gag gtc atc gag atg ctg gcg gcc cac gcc ggc 924
Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly
195 200 205
aag gag gcc gag gtc gcc gag gtc acc gag cgg gcc atg cgc ggc gag 972
Lys Glu Ala Glu Val Ala Glu Val Thr Glu Arg Ala Met Arg Gly Glu
210 215 220
ctc gac ttc gag gag tcc ctc cgt gag cgc gtc gcc acc ctc gag ggt 1020
Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Ala Thr Leu Glu Gly
225 230 235 240
ctc gac gcc tcc gtc atc gac gag gtc gcc cgc gac atc gtc ctg acc 1068
Leu Asp Ala Ser Val Ile Asp Glu Val Ala Arg Asp Ile Val Leu Thr
245 250 255
ccg ggc gcc cgc acg acc atc cgc acc ctg aag aag atg ggc tac cgc 1116
Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Lys Lys Met Gly Tyr Arg
260 265 270
acg gcc gtc gtc tcc ggt ggc ttc atc cag gtc ctc gag gac ctg gcc 1164
Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Asp Leu Ala
275 280 285
gag gac ctc cag ctc gac tac gtc cgc gcg aac acc ctg gag atc gtc 1212
Glu Asp Leu Gln Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val
290 295 300
gac ggc aag ctc acc ggc cgc gtg acc ggt gac atc gtc gac cgt gcc 1260
Asp Gly Lys Leu Thr Gly Arg Val Thr Gly Asp Ile Val Asp Arg Ala
305 310 315 320
cgc aag gcg gag ctg ctg cgc gag ttc gcc gag gac tcc ggc ctg cag 1308
Arg Lys Ala Glu Leu Leu Arg Glu Phe Ala Glu Asp Ser Gly Leu Gln
325 330 335
atg tac cag acc gtg gcc gtg ggt gac ggt gcc aac gac atc gac atg 1356
Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met
340 345 350
ctc tcc gcg gcg ggc ctg ggc atc gcg ttc aac gcc aag ccg gcg ctg 1404
Leu Ser Ala Ala Gly Leu Gly Ile Ala Phe Asn Ala Lys Pro Ala Leu
355 360 365
cgc gag atc gcc gac acc tcc gtc aac cac ccc ttc ctc gac gag gtg 1452
Arg Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val
370 375 380
ctc cac atc ctg ggc gtc ccg cgc gag gag atc gac gag tcc gac ctg 1500
Leu His Ile Leu Gly Val Pro Arg Glu Glu Ile Asp Glu Ser Asp Leu
385 390 395 400
gag gac ggc acc ttc cgc cgg gtc ccg ctc gag gtc tcc gca cgg tag 1548
Glu Asp Gly Thr Phe Arg Arg Val Pro Leu Glu Val Ser Ala Arg
405 410 415
tggaggagac cgatccgctc gccctcgcgc acgccctcct gagcgcgcgg gtgagcgaca 1608
accggcacga ccgctccgag aacccggagc agcaggagac cgtgcaggcg atgcagatcg 1668
ccctgcacgt gccgaagaca caaccgcccc gccgtaccga cctcctcgag gccgcggcgc 1728
gggcggttgt cgccgtctgc ctctccgaca gggtcgcggg ggacccgtcc tggcgggcgg 1788
ggctggaggg ctggtacgac cacctcatcc gcaaggtgac ccgccgggcc cgcaacaagg 1848
<210> 68
<211> 415
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 68
Val Ala Leu Gln Pro Gly Leu Glu Ser Ala Val Ile Thr Leu Thr Gly
1 5 10 15
Arg Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala
20 25 30
His Asp Val Gln Leu Leu Asp Val Glu Gln Ser Gln Phe Arg Gly Tyr
35 40 45
Leu Ser Leu Ala Ala Tyr Val Gly Val Asp Pro Glu Arg Val Asp Arg
50 55 60
Leu Arg Glu Gly Leu Thr Glu Thr Leu Lys Gly His Gly Gln Leu Ala
65 70 75 80
Thr Val Glu Met Val Pro Glu Gly Gln His Ser Ser Pro Arg Ser Thr
85 90 95
His Val Ala Val Val Leu Gly Asn Pro Val Thr Ala Thr Asp Val Ser
100 105 110
Ala Ile Gly Gln Thr Leu Ala Asn Tyr Gly Ala Asn Ile Asp Arg Ile
115 120 125
Arg Gly Ile Ala Asp Tyr Pro Ile Thr Gly Leu Glu Ile Met Ile Thr
130 135 140
Ile Ala Asn Pro Glu Pro Gly Gly Gly Lys Ala Met Arg Lys Ala Leu
145 150 155 160
Ala Glu Leu Thr Thr Glu Leu Gly Val Asp Ile Ala Ile Glu Arg Ala
165 170 175
Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Ile Phe Asp Cys Asp Ser
180 185 190
Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly
195 200 205
Lys Glu Ala Glu Val Ala Glu Val Thr Glu Arg Ala Met Arg Gly Glu
210 215 220
Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Ala Thr Leu Glu Gly
225 230 235 240
Leu Asp Ala Ser Val Ile Asp Glu Val Ala Arg Asp Ile Val Leu Thr
245 250 255
Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Lys Lys Met Gly Tyr Arg
260 265 270
Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Asp Leu Ala
275 280 285
Glu Asp Leu Gln Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val
290 295 300
Asp Gly Lys Leu Thr Gly Arg Val Thr Gly Asp Ile Val Asp Arg Ala
305 310 315 320
Arg Lys Ala Glu Leu Leu Arg Glu Phe Ala Glu Asp Ser Gly Leu Gln
325 330 335
Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met
340 345 350
Leu Ser Ala Ala Gly Leu Gly Ile Ala Phe Asn Ala Lys Pro Ala Leu
355 360 365
Arg Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val
370 375 380
Leu His Ile Leu Gly Val Pro Arg Glu Glu Ile Asp Glu Ser Asp Leu
385 390 395 400
Glu Asp Gly Thr Phe Arg Arg Val Pro Leu Glu Val Ser Ala Arg
405 410 415
<210> 69
<211> 1731
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1431)
<223> ген serC
<400> 69
ctgtgcagtt gtgctgccac cgcacggtgt ttgcgctgct cccttggggg gagggccgcc 60
gtccatgagg tggaacaggg cacagtttag caaatgatgt gatggaggca acagatttca 120
gaaaagcagg tcacccccat tgaatttgcg aggataagcc cctttgccta ccccagcaac 180
tacccccgtt gtcggtgtca accctaacac ccgggttccg gtcaacgggt gggaggggag 240
atactaaggt tgttctcgat tggacagcac ttcaccgacc acgaaaggga cgtcatctcc 300
atg acc gag ttc ccc acc ctc ccc gca gat ctc ctc ccg gca gac ggt 348
Met Thr Glu Phe Pro Thr Leu Pro Ala Asp Leu Leu Pro Ala Asp Gly
1 5 10 15
cgt ttc ggc tgc ggc ccc tcc aag gtc cgc ccg gag cag atc cag gcc 396
Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala
20 25 30
atc gtc gac ggc ggc cgc gac atc atc ggc acc tcg cac cgc cag ccg 444
Ile Val Asp Gly Gly Arg Asp Ile Ile Gly Thr Ser His Arg Gln Pro
35 40 45
gcc gtg aag aat gtc gtc ggt tcg gtc cgt gag ggc ctc tcc gac ctg 492
Ala Val Lys Asn Val Val Gly Ser Val Arg Glu Gly Leu Ser Asp Leu
50 55 60
ttc tcc ctg ccg gag ggc tac gag atc atc ctg tcc ctc ggt ggc gcc 540
Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala
65 70 75 80
acc gca ttc tgg gac gcc gcc acc ttc ggc ctc atc gag aag aag tcc 588
Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser
85 90 95
ggt cac ctg tcc tac ggt gag ttc tcc tcc aag ttc gcc aag gcc tcc 636
Gly His Leu Ser Tyr Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser
100 105 110
cag cag gca ccg tgg ctg gat gag ccg acc atc atc gag gcc ccg gcc 684
Gln Gln Ala Pro Trp Leu Asp Glu Pro Thr Ile Ile Glu Ala Pro Ala
115 120 125
ggc gac gcc ccg gag ccg cag gcc atg gag ggc acc gac gtc atc ggc 732
Gly Asp Ala Pro Glu Pro Gln Ala Met Glu Gly Thr Asp Val Ile Gly
130 135 140
tgg gcc cac aac gag acc tcc acc ggc gcc atg gtg ccg atc gtc cgc 780
Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Ile Val Arg
145 150 155 160
ccg gag ggc tcc gac ggc tcc ctc atc gtc atc gac gcc acc tcc ggc 828
Pro Glu Gly Ser Asp Gly Ser Leu Ile Val Ile Asp Ala Thr Ser Gly
165 170 175
gcc ggc ggc ctc ccg gtg aac atc gcc gac acc gac gtc tac tac ttc 876
Ala Gly Gly Leu Pro Val Asn Ile Ala Asp Thr Asp Val Tyr Tyr Phe
180 185 190
tcc ccg cag aag tgc ttc gcc tcc gac ggt ggc ctg tgg ctc gcc gcc 924
Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala
195 200 205
atg tcc ccg gcc gcc atg gag cgc atc gcg aag atc aag gag tcc ggc 972
Met Ser Pro Ala Ala Met Glu Arg Ile Ala Lys Ile Lys Glu Ser Gly
210 215 220
cgc cac atc ccg gcg ttc ctg gac ctg cag acc gcc gtc gag aac tcc 1020
Arg His Ile Pro Ala Phe Leu Asp Leu Gln Thr Ala Val Glu Asn Ser
225 230 235 240
ctg aag aac cag acc tac aac acc ccg gcc gtg ggc acc ctg ctc atg 1068
Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Gly Thr Leu Leu Met
245 250 255
ctg gac aac cag gtc aag tgg atg aac gag aac ggt ggc ctg gac ggc 1116
Leu Asp Asn Gln Val Lys Trp Met Asn Glu Asn Gly Gly Leu Asp Gly
260 265 270
atg gtc gcc cgc acc acc gcc tcc tcc gac gcc ctg tac aac tgg gcc 1164
Met Val Ala Arg Thr Thr Ala Ser Ser Asp Ala Leu Tyr Asn Trp Ala
275 280 285
gag gcc cgc gag gag acc acc ccg tac gtc acc gac aag gcg aag cgc 1212
Glu Ala Arg Glu Glu Thr Thr Pro Tyr Val Thr Asp Lys Ala Lys Arg
290 295 300
tcc ctc gtc gtc ggc acc atc gac ttc gac gac tcc gtc gac gcg gcg 1260
Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Val Asp Ala Ala
305 310 315 320
gtc gtc gcc aag gtc ctg cgt gcc aac ggc atc ctc gac gtc gag ccc 1308
Val Val Ala Lys Val Leu Arg Ala Asn Gly Ile Leu Asp Val Glu Pro
325 330 335
tac cgc aag ctg ggc cgc aac cag ctg cgc atc ggc atg ttc ccg gcg 1356
Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala
340 345 350
atc gac tcc cag gac atc gtg acc ctc acc aag gcg atc gac cac gtc 1404
Ile Asp Ser Gln Asp Ile Val Thr Leu Thr Lys Ala Ile Asp His Val
355 360 365
ctc gac tcg ggc gtc gcc gcc aag tag tcgccaggta gtcgcgggca 1451
Leu Asp Ser Gly Val Ala Ala Lys
370 375
cccgcacacc acccaacatg cgtcggacct cacacggtcc ggcgcatgtc tgcgttaggg 1511
tggtattcat cacgagagaa ggagaacgca tgcgcgagtt gttcctcgtc cctgaagagt 1571
cctcggccac ttccctggtg ttccggaccg aggacggcgg acagttcttc ctcgacgtca 1631
cttcactgga cgtggcctcc acccgtgcgc tcctgcttgc cgacgtcccg gaggacccct 1691
cccccacgga gtccggcacc gccgccgtca ccgccgtcga 1731
<210> 70
<211> 376
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 70
Met Thr Glu Phe Pro Thr Leu Pro Ala Asp Leu Leu Pro Ala Asp Gly
1 5 10 15
Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala
20 25 30
Ile Val Asp Gly Gly Arg Asp Ile Ile Gly Thr Ser His Arg Gln Pro
35 40 45
Ala Val Lys Asn Val Val Gly Ser Val Arg Glu Gly Leu Ser Asp Leu
50 55 60
Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala
65 70 75 80
Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser
85 90 95
Gly His Leu Ser Tyr Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser
100 105 110
Gln Gln Ala Pro Trp Leu Asp Glu Pro Thr Ile Ile Glu Ala Pro Ala
115 120 125
Gly Asp Ala Pro Glu Pro Gln Ala Met Glu Gly Thr Asp Val Ile Gly
130 135 140
Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Ile Val Arg
145 150 155 160
Pro Glu Gly Ser Asp Gly Ser Leu Ile Val Ile Asp Ala Thr Ser Gly
165 170 175
Ala Gly Gly Leu Pro Val Asn Ile Ala Asp Thr Asp Val Tyr Tyr Phe
180 185 190
Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala
195 200 205
Met Ser Pro Ala Ala Met Glu Arg Ile Ala Lys Ile Lys Glu Ser Gly
210 215 220
Arg His Ile Pro Ala Phe Leu Asp Leu Gln Thr Ala Val Glu Asn Ser
225 230 235 240
Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Gly Thr Leu Leu Met
245 250 255
Leu Asp Asn Gln Val Lys Trp Met Asn Glu Asn Gly Gly Leu Asp Gly
260 265 270
Met Val Ala Arg Thr Thr Ala Ser Ser Asp Ala Leu Tyr Asn Trp Ala
275 280 285
Glu Ala Arg Glu Glu Thr Thr Pro Tyr Val Thr Asp Lys Ala Lys Arg
290 295 300
Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Val Asp Ala Ala
305 310 315 320
Val Val Ala Lys Val Leu Arg Ala Asn Gly Ile Leu Asp Val Glu Pro
325 330 335
Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala
340 345 350
Ile Asp Ser Gln Asp Ile Val Thr Leu Thr Lys Ala Ile Asp His Val
355 360 365
Leu Asp Ser Gly Val Ala Ala Lys
370 375
<210> 71
<211> 1665
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1365)
<223> ген аланиндегидрогеназы
<400> 71
acctctcgct cagccacctg cgccgcgcga cgaactcctc gcggaagtag ccgaggtggg 60
cggtcggccc gccgaaggcg gtgacgccca gcagcccgaa gctgcggatg acctctcctg 120
tggtgccgtt gttcttgtct gcggattcct tcacgctcgt catggtaggg cggggcgtcg 180
agaagtgcgc gtcgagcgac agtaaccggg attacatcat taaccagccg gttccgacga 240
gcgtccggaa ggcgcctagt agagtcagtg gtcatgcgta tcggaatccc gaaggaaatc 300
atg aac atg gag ggc cgc gtg gcc ctc agc ccg ctc ggg gca ggc acc 348
Met Asn Met Glu Gly Arg Val Ala Leu Ser Pro Leu Gly Ala Gly Thr
1 5 10 15
ctc gta gcc gac ggc cat gag gtg att gtt cag gcc ggg gcg ggc gag 396
Leu Val Ala Asp Gly His Glu Val Ile Val Gln Ala Gly Ala Gly Glu
20 25 30
aac tcc tcg ttc acc gat gcc gag tac gag gcg acg ggc gcg cgc gtc 444
Asn Ser Ser Phe Thr Asp Ala Glu Tyr Glu Ala Thr Gly Ala Arg Val
35 40 45
gtc gcc tcc gcg gag gag gcc tgg ggc gcc gag atg gtg ctc aag gtc 492
Val Ala Ser Ala Glu Glu Ala Trp Gly Ala Glu Met Val Leu Lys Val
50 55 60
aag gag ccg ctg ccg gag gag tac ggc ttc ctc cgc ccg gac ctc atc 540
Lys Glu Pro Leu Pro Glu Glu Tyr Gly Phe Leu Arg Pro Asp Leu Ile
65 70 75 80
ctg ttc acc tac ctg cac ctg gcg tcc tcg cgt gag tgc acg aag gcg 588
Leu Phe Thr Tyr Leu His Leu Ala Ser Ser Arg Glu Cys Thr Lys Ala
85 90 95
ctg ctc gac gcc ggc acc ctg tcc ctg gcg tac gag acg gtc ctc gac 636
Leu Leu Asp Ala Gly Thr Leu Ser Leu Ala Tyr Glu Thr Val Leu Asp
100 105 110
gcc cgc ggc ggc ctc ccg ctg ctc acc ccg atg tcc cag gtc gcg ggc 684
Ala Arg Gly Gly Leu Pro Leu Leu Thr Pro Met Ser Gln Val Ala Gly
115 120 125
cgc ctc gcc gtg cag gag ggc gcg cac cac ctc atg agc tcc aag ggg 732
Arg Leu Ala Val Gln Glu Gly Ala His His Leu Met Ser Ser Lys Gly
130 135 140
ggc cgc ggc gtg ctg ctc ggt ggc gtg ccc ggc acc gcc ccg gcg aag 780
Gly Arg Gly Val Leu Leu Gly Gly Val Pro Gly Thr Ala Pro Ala Lys
145 150 155 160
gtc gtc gtc ctc ggc ggc ggt cag gtc ggc gcc tcg gcc gtc gcg atc 828
Val Val Val Leu Gly Gly Gly Gln Val Gly Ala Ser Ala Val Ala Ile
165 170 175
gcg cag ggt ctg cgc gcc agc gtc acg gtc ctc gac ctc gac ccg cag 876
Ala Gln Gly Leu Arg Ala Ser Val Thr Val Leu Asp Leu Asp Pro Gln
180 185 190
gtg ctc gcg cgt ttc gac gac ctc tac cag ggc aac gtc cgc acc ctg 924
Val Leu Ala Arg Phe Asp Asp Leu Tyr Gln Gly Asn Val Arg Thr Leu
195 200 205
ctg tcc gac ccg atg acc atc gag acc gag ctt ctc gac gcc gac ctc 972
Leu Ser Asp Pro Met Thr Ile Glu Thr Glu Leu Leu Asp Ala Asp Leu
210 215 220
gtc atc ggc gcc gtc ctc gtc gcc ggc gcg aag gcc ccg acg ctg gtc 1020
Val Ile Gly Ala Val Leu Val Ala Gly Ala Lys Ala Pro Thr Leu Val
225 230 235 240
agc gac gac ctg gtc gcc cgc atg aag cag ggc gcc gtg ctc gtc gac 1068
Ser Asp Asp Leu Val Ala Arg Met Lys Gln Gly Ala Val Leu Val Asp
245 250 255
gtc gcc atc gac cag ggc ggc tgc ttc gag ccc tcc cgc aag acc tcc 1116
Val Ala Ile Asp Gln Gly Gly Cys Phe Glu Pro Ser Arg Lys Thr Ser
260 265 270
cac gag gac ccg acc tac gag gtc cac ggc acc cgc ttc tac tgc gtg 1164
His Glu Asp Pro Thr Tyr Glu Val His Gly Thr Arg Phe Tyr Cys Val
275 280 285
ccc aac atg ccc ggc gcg gtg gcc aac acc tcg acc cgg gcg ctc acc 1212
Pro Asn Met Pro Gly Ala Val Ala Asn Thr Ser Thr Arg Ala Leu Thr
290 295 300
tcg gcg acc ctg ccc tac gtc cgc gcg atc gcg gcg ggc ggc gtg gac 1260
Ser Ala Thr Leu Pro Tyr Val Arg Ala Ile Ala Ala Gly Gly Val Asp
305 310 315 320
cgg gcg gtg gac aac cac ccg ggt ctg gag ccg ggc atc atg acc tgc 1308
Arg Ala Val Asp Asn His Pro Gly Leu Glu Pro Gly Ile Met Thr Cys
325 330 335
gac ggc cgc ctg atc agt gag cca gtg aag gag gcg ttc ccg gat ctt 1356
Asp Gly Arg Leu Ile Ser Glu Pro Val Lys Glu Ala Phe Pro Asp Leu
340 345 350
ctc ggt tga tgagcatcgc cgccacccgc tccatgtgat cccagatctg 1405
Leu Gly
gtggcggtac ccggcgggga tggtctcctc gtcgatgctg tcgagtgaca ccttcatcag 1465
ctccagccag cggatcgcag ccgcgcggtc cacggggaac tcgtggtggc ggcggcgcag 1525
catcggggcg ccgcgcttct cgttgaaggt ggccggtccg ccccagtact gggcgaggaa 1585
ccacttgagg cggttctcgg cgccctccca gtcgtcctcg gggtacatgg gccccaggat 1645
gtcgtccgtc ttcacctgcg 1665
<210> 72
<211> 354
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 72
Met Asn Met Glu Gly Arg Val Ala Leu Ser Pro Leu Gly Ala Gly Thr
1 5 10 15
Leu Val Ala Asp Gly His Glu Val Ile Val Gln Ala Gly Ala Gly Glu
20 25 30
Asn Ser Ser Phe Thr Asp Ala Glu Tyr Glu Ala Thr Gly Ala Arg Val
35 40 45
Val Ala Ser Ala Glu Glu Ala Trp Gly Ala Glu Met Val Leu Lys Val
50 55 60
Lys Glu Pro Leu Pro Glu Glu Tyr Gly Phe Leu Arg Pro Asp Leu Ile
65 70 75 80
Leu Phe Thr Tyr Leu His Leu Ala Ser Ser Arg Glu Cys Thr Lys Ala
85 90 95
Leu Leu Asp Ala Gly Thr Leu Ser Leu Ala Tyr Glu Thr Val Leu Asp
100 105 110
Ala Arg Gly Gly Leu Pro Leu Leu Thr Pro Met Ser Gln Val Ala Gly
115 120 125
Arg Leu Ala Val Gln Glu Gly Ala His His Leu Met Ser Ser Lys Gly
130 135 140
Gly Arg Gly Val Leu Leu Gly Gly Val Pro Gly Thr Ala Pro Ala Lys
145 150 155 160
Val Val Val Leu Gly Gly Gly Gln Val Gly Ala Ser Ala Val Ala Ile
165 170 175
Ala Gln Gly Leu Arg Ala Ser Val Thr Val Leu Asp Leu Asp Pro Gln
180 185 190
Val Leu Ala Arg Phe Asp Asp Leu Tyr Gln Gly Asn Val Arg Thr Leu
195 200 205
Leu Ser Asp Pro Met Thr Ile Glu Thr Glu Leu Leu Asp Ala Asp Leu
210 215 220
Val Ile Gly Ala Val Leu Val Ala Gly Ala Lys Ala Pro Thr Leu Val
225 230 235 240
Ser Asp Asp Leu Val Ala Arg Met Lys Gln Gly Ala Val Leu Val Asp
245 250 255
Val Ala Ile Asp Gln Gly Gly Cys Phe Glu Pro Ser Arg Lys Thr Ser
260 265 270
His Glu Asp Pro Thr Tyr Glu Val His Gly Thr Arg Phe Tyr Cys Val
275 280 285
Pro Asn Met Pro Gly Ala Val Ala Asn Thr Ser Thr Arg Ala Leu Thr
290 295 300
Ser Ala Thr Leu Pro Tyr Val Arg Ala Ile Ala Ala Gly Gly Val Asp
305 310 315 320
Arg Ala Val Asp Asn His Pro Gly Leu Glu Pro Gly Ile Met Thr Cys
325 330 335
Asp Gly Arg Leu Ile Ser Glu Pro Val Lys Glu Ala Phe Pro Asp Leu
340 345 350
Leu Gly
<210> 73
<211> 1515
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1215)
<223> ген cysD
<400> 73
cctgaccccg tacgcggggt gggtcaccgg cctgcgccgc gctgacggac cgacccgggc 60
gcacgccccg gcgctcagcc ttgacgcgac ggggcggctg aagatctcgc cgctggtcac 120
gtggtccctc gtggacacgg aggcgttcat cgcggaccac gacctcatcc gacaccccct 180
gaccacgcag ggctacccgt ccatcgggtg cgccacctgc accctccccg tcgccgacgg 240
ggaggatccc cgggccggcc gctgggccgg acagaacaag acagaatgcg ggctccactc 300
atg acc acc acc acc ctc acc aga ctc tcc ccc cat ctg cag gac ctc 348
Met Thr Thr Thr Thr Leu Thr Arg Leu Ser Pro His Leu Gln Asp Leu
1 5 10 15
gag aac gag tcc atc cac atc ctc cgg gag gtg gcc ggg cag ttc gac 396
Glu Asn Glu Ser Ile His Ile Leu Arg Glu Val Ala Gly Gln Phe Asp
20 25 30
cgg gtc ggg ctg ctg ttc tcc ggc ggc aag gac tcc gtc gtc gtg ctc 444
Arg Val Gly Leu Leu Phe Ser Gly Gly Lys Asp Ser Val Val Val Leu
35 40 45
gag ctg gcg cgc cgg gcc ttc gct ccg gcg gcc gtg ccc ttc gag ctg 492
Glu Leu Ala Arg Arg Ala Phe Ala Pro Ala Ala Val Pro Phe Glu Leu
50 55 60
ctc cac gtg gac acc ggc cac aac ttc ccc gag gtc atc gag ttc cgt 540
Leu His Val Asp Thr Gly His Asn Phe Pro Glu Val Ile Glu Phe Arg
65 70 75 80
gac cga gtc gtc gcc gag acc ggc gtg cgg ctg cgc gtc gcg cac gtg 588
Asp Arg Val Val Ala Glu Thr Gly Val Arg Leu Arg Val Ala His Val
85 90 95
cag gac tgg atc gac cgc ggt gac ctc gtc gaa cgt ccc gac ggc acc 636
Gln Asp Trp Ile Asp Arg Gly Asp Leu Val Glu Arg Pro Asp Gly Thr
100 105 110
cgc aat ccc ctg cag acc gtc ccc ctc gtg gag acc atc gcc gag cag 684
Arg Asn Pro Leu Gln Thr Val Pro Leu Val Glu Thr Ile Ala Glu Gln
115 120 125
ggc tac gac gcc gtc ctc ggt ggc gcc cgc cgc gac gag gag cgg gcg 732
Gly Tyr Asp Ala Val Leu Gly Gly Ala Arg Arg Asp Glu Glu Arg Ala
130 135 140
cgc gcc aag gag cgt gtc ttc tcc gtg cgc gac tcc ttc ggc ggc tgg 780
Arg Ala Lys Glu Arg Val Phe Ser Val Arg Asp Ser Phe Gly Gly Trp
145 150 155 160
gat ccg cgc cgc cag cgc ccg gag ctg tgg cac ctc tac aac ggc ggc 828
Asp Pro Arg Arg Gln Arg Pro Glu Leu Trp His Leu Tyr Asn Gly Gly
165 170 175
cac ctg ccg ggc gag aac gtc cgc gtc ttc ccc atc tcc aac tgg acc 876
His Leu Pro Gly Glu Asn Val Arg Val Phe Pro Ile Ser Asn Trp Thr
180 185 190
gag gcc gac atc tgg gag tac atc ggc gcc cgc ggc atc gcg ctg ccg 924
Glu Ala Asp Ile Trp Glu Tyr Ile Gly Ala Arg Gly Ile Ala Leu Pro
195 200 205
gag atc tac ttc gcg cat gag cgg gcc gtc ttc gag cgg gac ggc atg 972
Glu Ile Tyr Phe Ala His Glu Arg Ala Val Phe Glu Arg Asp Gly Met
210 215 220
tgg ctg acc gcc ggc gag tgg ggt ggg ccc cgc gag ggc gag acc ctc 1020
Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Arg Glu Gly Glu Thr Leu
225 230 235 240
gag gtc cgc cgc gtg cgc tac cgc acc gtc ggc gac atg tcc tgc acc 1068
Glu Val Arg Arg Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys Thr
245 250 255
ggt gcg gtg ctc tcc gac gcc acc acg gtc gac gac gtc atc gcc gag 1116
Gly Ala Val Leu Ser Asp Ala Thr Thr Val Asp Asp Val Ile Ala Glu
260 265 270
atc gcc acc tcc acc ctc acc gag cgc ggg gcc acc cgc gcc gac gac 1164
Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp Asp
275 280 285
cgc ctc agt gaa tcc gcc atg gaa gac cgc aag aag gaa ggc tac ttc 1212
Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr Phe
290 295 300
tga tgaccaccac gctcctgcgc acccgcgaga cgctgcgcct gtgcaccgcc 1265
ggctccgtcg acgacggcaa gtccaccctc gtgggccgcc tgctccacga cacccgctcc 1325
gtcctcgccg accagctcgc cgccgtcgag cgcacctccg ccgaccgcgg tttccacggc 1385
ggcctggacc tctcactgct cgtcgacggc ctccgcgccg aacgcgagca gggcatcacc 1445
atcgacgtgg cctaccgcta cttcgccacc gaccgccgca ccttcatcct cgccgacacc 1505
cccggccacg 1515
<210> 74
<211> 304
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 74
Met Thr Thr Thr Thr Leu Thr Arg Leu Ser Pro His Leu Gln Asp Leu
1 5 10 15
Glu Asn Glu Ser Ile His Ile Leu Arg Glu Val Ala Gly Gln Phe Asp
20 25 30
Arg Val Gly Leu Leu Phe Ser Gly Gly Lys Asp Ser Val Val Val Leu
35 40 45
Glu Leu Ala Arg Arg Ala Phe Ala Pro Ala Ala Val Pro Phe Glu Leu
50 55 60
Leu His Val Asp Thr Gly His Asn Phe Pro Glu Val Ile Glu Phe Arg
65 70 75 80
Asp Arg Val Val Ala Glu Thr Gly Val Arg Leu Arg Val Ala His Val
85 90 95
Gln Asp Trp Ile Asp Arg Gly Asp Leu Val Glu Arg Pro Asp Gly Thr
100 105 110
Arg Asn Pro Leu Gln Thr Val Pro Leu Val Glu Thr Ile Ala Glu Gln
115 120 125
Gly Tyr Asp Ala Val Leu Gly Gly Ala Arg Arg Asp Glu Glu Arg Ala
130 135 140
Arg Ala Lys Glu Arg Val Phe Ser Val Arg Asp Ser Phe Gly Gly Trp
145 150 155 160
Asp Pro Arg Arg Gln Arg Pro Glu Leu Trp His Leu Tyr Asn Gly Gly
165 170 175
His Leu Pro Gly Glu Asn Val Arg Val Phe Pro Ile Ser Asn Trp Thr
180 185 190
Glu Ala Asp Ile Trp Glu Tyr Ile Gly Ala Arg Gly Ile Ala Leu Pro
195 200 205
Glu Ile Tyr Phe Ala His Glu Arg Ala Val Phe Glu Arg Asp Gly Met
210 215 220
Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Arg Glu Gly Glu Thr Leu
225 230 235 240
Glu Val Arg Arg Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys Thr
245 250 255
Gly Ala Val Leu Ser Asp Ala Thr Thr Val Asp Asp Val Ile Ala Glu
260 265 270
Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp Asp
275 280 285
Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr Phe
290 295 300
<210> 75
<211> 1575
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1275)
<223> ген cysH
<400> 75
cgtgcgcatc gccgtcaacg gctgccccaa ctcgtgtgcc cgcacgcagg tcgcggacat 60
cggcttcaag ggccagaccg tcaccgacgc cgacggcaac cgggtcgagg gcttccaggt 120
gcacctcggc gggtcgatga gcctcaacgc caacttcggc cgcaagctcc gcggccacaa 180
ggtgctcgcc gacgacatcg gggactacgt cacccgcgtg gtcacccact tccgggacca 240
gcgccgaccg gaggagacct tccaggagtg ggtccagcgc gccgccgagg aggacctcca 300
gtg agt ttc cgt cgc gcc ccc aac ccg aac cgc aac cat ccg ctg cac 348
Val Ser Phe Arg Arg Ala Pro Asn Pro Asn Arg Asn His Pro Leu His
1 5 10 15
tgc ccg tgg tgc gcc ggc gag gat ctg ctg ccc aac acg gag gac gac 396
Cys Pro Trp Cys Ala Gly Glu Asp Leu Leu Pro Asn Thr Glu Asp Asp
20 25 30
ttc gcc tgg ctg tgc cgc gac tgc acc cgg gtg ttc tcc gtc cgc tac 444
Phe Ala Trp Leu Cys Arg Asp Cys Thr Arg Val Phe Ser Val Arg Tyr
35 40 45
cac ggg cag gac gcc ccg gcg cgc cga ccg gcc ccg gca ctc tcc acg 492
His Gly Gln Asp Ala Pro Ala Arg Arg Pro Ala Pro Ala Leu Ser Thr
50 55 60
agt gcg gcg atc cag ctc tcc ctg tcc cgt cgc ggc cac ctc aac gac 540
Ser Ala Ala Ile Gln Leu Ser Leu Ser Arg Arg Gly His Leu Asn Asp
65 70 75 80
ccc gcc gtc agc ccg gca ggc ccc acc gcc acc ggg gac ctg ccg ggg 588
Pro Ala Val Ser Pro Ala Gly Pro Thr Ala Thr Gly Asp Leu Pro Gly
85 90 95
gcg gtg cgg gag gag aac gcg cgg ctc gtc gag aag cac gcg gat gag 636
Ala Val Arg Glu Glu Asn Ala Arg Leu Val Glu Lys His Ala Asp Glu
100 105 110
ctg tac gac gcc ccc gcc gac gtc atc ctg gcg tgg gcc cgg gag cac 684
Leu Tyr Asp Ala Pro Ala Asp Val Ile Leu Ala Trp Ala Arg Glu His
115 120 125
gtg ccg ggt cgc ctc gcg gtg acg ctc tcg atg gag aac acc gtc ctc 732
Val Pro Gly Arg Leu Ala Val Thr Leu Ser Met Glu Asn Thr Val Leu
130 135 140
gcg gag ctg gcc gcc cgg cac acg ccc gac gcc gac ctg ctc ttc ctc 780
Ala Glu Leu Ala Ala Arg His Thr Pro Asp Ala Asp Leu Leu Phe Leu
145 150 155 160
gac acc ggc tac cac ttc gag gag acg ctg gcg gtg gcg cgg gac gtc 828
Asp Thr Gly Tyr His Phe Glu Glu Thr Leu Ala Val Ala Arg Asp Val
165 170 175
gac agg cgg tac ccg cag acg ctg gtg acc gcg acg ccg acg ctc acc 876
Asp Arg Arg Tyr Pro Gln Thr Leu Val Thr Ala Thr Pro Thr Leu Thr
180 185 190
cgg gcg gag cag gac gcc gcc tac ggg ccc ggc ctg tac cgg cgc gac 924
Arg Ala Glu Gln Asp Ala Ala Tyr Gly Pro Gly Leu Tyr Arg Arg Asp
195 200 205
ccg gcc gcg tgc tgc cgc atg cgc aag gtc gag ccg ctg gcc gtg cac 972
Pro Ala Ala Cys Cys Arg Met Arg Lys Val Glu Pro Leu Ala Val His
210 215 220
ctg acc ccg tac gcg ggg tgg gtc acc ggc ctg cgc cgc gct gac gga 1020
Leu Thr Pro Tyr Ala Gly Trp Val Thr Gly Leu Arg Arg Ala Asp Gly
225 230 235 240
ccg acc cgg gcg cac gcc ccg gcg ctc agc ctt gac gcg acg ggg cgg 1068
Pro Thr Arg Ala His Ala Pro Ala Leu Ser Leu Asp Ala Thr Gly Arg
245 250 255
ctg aag atc tcg ccg ctg gtc acg tgg tcc ctc gtg gac acg gag gcg 1116
Leu Lys Ile Ser Pro Leu Val Thr Trp Ser Leu Val Asp Thr Glu Ala
260 265 270
ttc atc gcg gac cac gac ctc atc cga cac ccc ctg acc acg cag ggc 1164
Phe Ile Ala Asp His Asp Leu Ile Arg His Pro Leu Thr Thr Gln Gly
275 280 285
tac ccg tcc atc ggg tgc gcc acc tgc acc ctc ccc gtc gcc gac ggg 1212
Tyr Pro Ser Ile Gly Cys Ala Thr Cys Thr Leu Pro Val Ala Asp Gly
290 295 300
gag gat ccc cgg gcc ggc cgc tgg gcc gga cag aac aag aca gaa tgc 1260
Glu Asp Pro Arg Ala Gly Arg Trp Ala Gly Gln Asn Lys Thr Glu Cys
305 310 315 320
ggg ctc cac tca tga ccaccaccac cctcaccaga ctctcccccc atctgcagga 1315
Gly Leu His Ser
cctcgagaac gagtccatcc acatcctccg ggaggtggcc gggcagttcg accgggtcgg 1375
gctgctgttc tccggcggca aggactccgt cgtcgtgctc gagctggcgc gccgggcctt 1435
cgctccggcg gccgtgccct tcgagctgct ccacgtggac accggccaca acttccccga 1495
ggtcatcgag ttccgtgacc gagtcgtcgc cgagaccggc gtgcggctgc gcgtcgcgca 1555
cgtgcaggac tggatcgacc 1575
<210> 76
<211> 324
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 76
Val Ser Phe Arg Arg Ala Pro Asn Pro Asn Arg Asn His Pro Leu His
1 5 10 15
Cys Pro Trp Cys Ala Gly Glu Asp Leu Leu Pro Asn Thr Glu Asp Asp
20 25 30
Phe Ala Trp Leu Cys Arg Asp Cys Thr Arg Val Phe Ser Val Arg Tyr
35 40 45
His Gly Gln Asp Ala Pro Ala Arg Arg Pro Ala Pro Ala Leu Ser Thr
50 55 60
Ser Ala Ala Ile Gln Leu Ser Leu Ser Arg Arg Gly His Leu Asn Asp
65 70 75 80
Pro Ala Val Ser Pro Ala Gly Pro Thr Ala Thr Gly Asp Leu Pro Gly
85 90 95
Ala Val Arg Glu Glu Asn Ala Arg Leu Val Glu Lys His Ala Asp Glu
100 105 110
Leu Tyr Asp Ala Pro Ala Asp Val Ile Leu Ala Trp Ala Arg Glu His
115 120 125
Val Pro Gly Arg Leu Ala Val Thr Leu Ser Met Glu Asn Thr Val Leu
130 135 140
Ala Glu Leu Ala Ala Arg His Thr Pro Asp Ala Asp Leu Leu Phe Leu
145 150 155 160
Asp Thr Gly Tyr His Phe Glu Glu Thr Leu Ala Val Ala Arg Asp Val
165 170 175
Asp Arg Arg Tyr Pro Gln Thr Leu Val Thr Ala Thr Pro Thr Leu Thr
180 185 190
Arg Ala Glu Gln Asp Ala Ala Tyr Gly Pro Gly Leu Tyr Arg Arg Asp
195 200 205
Pro Ala Ala Cys Cys Arg Met Arg Lys Val Glu Pro Leu Ala Val His
210 215 220
Leu Thr Pro Tyr Ala Gly Trp Val Thr Gly Leu Arg Arg Ala Asp Gly
225 230 235 240
Pro Thr Arg Ala His Ala Pro Ala Leu Ser Leu Asp Ala Thr Gly Arg
245 250 255
Leu Lys Ile Ser Pro Leu Val Thr Trp Ser Leu Val Asp Thr Glu Ala
260 265 270
Phe Ile Ala Asp His Asp Leu Ile Arg His Pro Leu Thr Thr Gln Gly
275 280 285
Tyr Pro Ser Ile Gly Cys Ala Thr Cys Thr Leu Pro Val Ala Asp Gly
290 295 300
Glu Asp Pro Arg Ala Gly Arg Trp Ala Gly Gln Asn Lys Thr Glu Cys
305 310 315 320
Gly Leu His Ser
<210> 77
<211> 2295
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1995)
<223> ген cysI
<400> 77
gaaagtagca cccctgggcg gtaatgcaag cgaaaaggcg acttcaattc agggtccggg 60
catcgctctt cgacgccccc aattcaattc agtgaatgta tgtcggatat aaacactacg 120
tacagccatg atcgggaaag ttgcccaaaa tcatgacgta tctacatctc tccaccattg 180
ccatgaactg atctgtctgc tctaatccac tgcagacgcc ccggcagggg ctccgtggcg 240
gcgcccggcg ccgaatccac gtgaatgaaa tagacagaac agttcagtag ggagatggtc 300
atg gac acg acc acc acg atc gag aag gca ccc cgc cgc gag cgc ccc 348
Met Asp Thr Thr Thr Thr Ile Glu Lys Ala Pro Arg Arg Glu Arg Pro
1 5 10 15
aga cgc gcc cgc aag ccg gag ggc cag tgg cgg atc gac ggc acc acg 396
Arg Arg Ala Arg Lys Pro Glu Gly Gln Trp Arg Ile Asp Gly Thr Thr
20 25 30
ccg ctc aac cac gtg gag gag gtg aag cag gac ggc gac atc ctc gcc 444
Pro Leu Asn His Val Glu Glu Val Lys Gln Asp Gly Asp Ile Leu Ala
35 40 45
gtc tac gac cgg gtg gtc aac atc tac tcg aag cag ggt ttc gac tcc 492
Val Tyr Asp Arg Val Val Asn Ile Tyr Ser Lys Gln Gly Phe Asp Ser
50 55 60
atc ccc gcc gac gac ctc gcc ccg cgc ttc aag tgg ctg ggc ctg tac 540
Ile Pro Ala Asp Asp Leu Ala Pro Arg Phe Lys Trp Leu Gly Leu Tyr
65 70 75 80
acg cag cgc cgc cag gat ctc ggc ggt gag ctc acc ggc cag ctg ccc 588
Thr Gln Arg Arg Gln Asp Leu Gly Gly Glu Leu Thr Gly Gln Leu Pro
85 90 95
gac gcc gag ctg cag gac cgc tac ttc atg atg cgc gtg cgt ttc gag 636
Asp Ala Glu Leu Gln Asp Arg Tyr Phe Met Met Arg Val Arg Phe Glu
100 105 110
ggc ggc cag gta acc ccg gcc cgc ctc cgc gcg gtg ggg gag atc tcc 684
Gly Gly Gln Val Thr Pro Ala Arg Leu Arg Ala Val Gly Glu Ile Ser
115 120 125
cgc gac tac gcc cgc agc acc gcg gac ttc acc gat cgg cag aac atc 732
Arg Asp Tyr Ala Arg Ser Thr Ala Asp Phe Thr Asp Arg Gln Asn Ile
130 135 140
cag ctg cac tgg ctg cgc atc gag gac atc ccg gcg atc tgg gag aag 780
Gln Leu His Trp Leu Arg Ile Glu Asp Ile Pro Ala Ile Trp Glu Lys
145 150 155 160
ctc gac tcc gtg ggg ctc agc ccc ctg ctg ggc tgc ggt gac gtg ccg 828
Leu Asp Ser Val Gly Leu Ser Pro Leu Leu Gly Cys Gly Asp Val Pro
165 170 175
cgc atc atc ctc ggc tcc ccg gtc gcg ggg gtc gcc gcc gag gag atc 876
Arg Ile Ile Leu Gly Ser Pro Val Ala Gly Val Ala Ala Glu Glu Ile
180 185 190
atc gac gcc acc ccg gcg atc gag cgg atc cag cgc gag cac ctg ccg 924
Ile Asp Ala Thr Pro Ala Ile Glu Arg Ile Gln Arg Glu His Leu Pro
195 200 205
aac ccg gag ttc cac aac ctg ccg cgc aag ttc aag tcc gcg atc tcc 972
Asn Pro Glu Phe His Asn Leu Pro Arg Lys Phe Lys Ser Ala Ile Ser
210 215 220
ggc cac tcg cgc cag gac gtc acc cac gag atc cag gac gtc gcc ttc 1020
Gly His Ser Arg Gln Asp Val Thr His Glu Ile Gln Asp Val Ala Phe
225 230 235 240
atc ggt tcc gtc cac ccc gag cac ggc ccg ggt ttc gag tgc ttc gtc 1068
Ile Gly Ser Val His Pro Glu His Gly Pro Gly Phe Glu Cys Phe Val
245 250 255
ggc ggt ggc ctg tcc acc aac ccg atg ctc gcg cag tcg ctc ggc gcg 1116
Gly Gly Gly Leu Ser Thr Asn Pro Met Leu Ala Gln Ser Leu Gly Ala
260 265 270
tgg atc ccg ctg gag gac gtg ccg gac gtg tgg gcc ggc gtc gcc cgg 1164
Trp Ile Pro Leu Glu Asp Val Pro Asp Val Trp Ala Gly Val Ala Arg
275 280 285
atc ttc cgc gac tac ggt tac cgc cgc ctg cgg aat cgt gcc cgt ctc 1212
Ile Phe Arg Asp Tyr Gly Tyr Arg Arg Leu Arg Asn Arg Ala Arg Leu
290 295 300
aag ttc ctc gtg gcg gaa tgg ggg atc gag aag ttc cgg gac gtc ctc 1260
Lys Phe Leu Val Ala Glu Trp Gly Ile Glu Lys Phe Arg Asp Val Leu
305 310 315 320
gag acg gag tac ctc gga cgc ccg ctc atc gac gga ccc cgc cag ccc 1308
Glu Thr Glu Tyr Leu Gly Arg Pro Leu Ile Asp Gly Pro Arg Gln Pro
325 330 335
acc aac ccc ggc tac cgc gac cac atc ggc atc cac ccg cag aag gac 1356
Thr Asn Pro Gly Tyr Arg Asp His Ile Gly Ile His Pro Gln Lys Asp
340 345 350
ggc cgc ttc tac ctg ggc gtg aag ccg acc gtc ggg cac acc acc ggt 1404
Gly Arg Phe Tyr Leu Gly Val Lys Pro Thr Val Gly His Thr Thr Gly
355 360 365
gag cag ctc atc gcc atc gcc gag gtg gcg gag cgt ttc ggc atc gag 1452
Glu Gln Leu Ile Ala Ile Ala Glu Val Ala Glu Arg Phe Gly Ile Glu
370 375 380
cgc atc cgc acc acc tcg gag aag gag ctg ctc ttc ctc gac gtc gag 1500
Arg Ile Arg Thr Thr Ser Glu Lys Glu Leu Leu Phe Leu Asp Val Glu
385 390 395 400
cgc acc gac ctc acc gcc ctg gcc gcc gcc ctc gac gag acc ggc ctg 1548
Arg Thr Asp Leu Thr Ala Leu Ala Ala Ala Leu Asp Glu Thr Gly Leu
405 410 415
tac tcg aag ccg agc gag tgg cgc cgc aac atc atc tcg tgc acc ggc 1596
Tyr Ser Lys Pro Ser Glu Trp Arg Arg Asn Ile Ile Ser Cys Thr Gly
420 425 430
ctg gag ttc tgc aaa ctg gcg cac gcg acg acc aag tcg cgt gcc gtg 1644
Leu Glu Phe Cys Lys Leu Ala His Ala Thr Thr Lys Ser Arg Ala Val
435 440 445
gcc ctg gtc gac gag ctg gag gaa cgc gtc ggc gac atc gac gtg ccc 1692
Ala Leu Val Asp Glu Leu Glu Glu Arg Val Gly Asp Ile Asp Val Pro
450 455 460
gtg cgc atc gcc gtc aac ggc tgc ccc aac tcg tgt gcc cgc acg cag 1740
Val Arg Ile Ala Val Asn Gly Cys Pro Asn Ser Cys Ala Arg Thr Gln
465 470 475 480
gtc gcg gac atc ggc ttc aag ggc cag acc gtc acc gac gcc gac ggc 1788
Val Ala Asp Ile Gly Phe Lys Gly Gln Thr Val Thr Asp Ala Asp Gly
485 490 495
aac cgg gtc gag ggc ttc cag gtg cac ctc ggc ggg tcg atg agc ctc 1836
Asn Arg Val Glu Gly Phe Gln Val His Leu Gly Gly Ser Met Ser Leu
500 505 510
aac gcc aac ttc ggc cgc aag ctc cgc ggc cac aag gtg ctc gcc gac 1884
Asn Ala Asn Phe Gly Arg Lys Leu Arg Gly His Lys Val Leu Ala Asp
515 520 525
gac atc ggg gac tac gtc acc cgc gtg gtc acc cac ttc cgg gac cag 1932
Asp Ile Gly Asp Tyr Val Thr Arg Val Val Thr His Phe Arg Asp Gln
530 535 540
cgc cga ccg gag gag acc ttc cag gag tgg gtc cag cgc gcc gcc gag 1980
Arg Arg Pro Glu Glu Thr Phe Gln Glu Trp Val Gln Arg Ala Ala Glu
545 550 555 560
gag gac ctc cag tga gtttccgtcg cgcccccaac ccgaaccgca accatccgct 2035
Glu Asp Leu Gln
gcactgcccg tggtgcgccg gcgaggatct gctgcccaac acggaggacg acttcgcctg 2095
gctgtgccgc gactgcaccc gggtgttctc cgtccgctac cacgggcagg acgccccggc 2155
gcgccgaccg gccccggcac tctccacgag tgcggcgatc cagctctccc tgtcccgtcg 2215
cggccacctc aacgaccccg ccgtcagccc ggcaggcccc accgccaccg gggacctgcc 2275
gggggcggtg cgggaggaga 2295
<210> 78
<211> 564
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 78
Met Asp Thr Thr Thr Thr Ile Glu Lys Ala Pro Arg Arg Glu Arg Pro
1 5 10 15
Arg Arg Ala Arg Lys Pro Glu Gly Gln Trp Arg Ile Asp Gly Thr Thr
20 25 30
Pro Leu Asn His Val Glu Glu Val Lys Gln Asp Gly Asp Ile Leu Ala
35 40 45
Val Tyr Asp Arg Val Val Asn Ile Tyr Ser Lys Gln Gly Phe Asp Ser
50 55 60
Ile Pro Ala Asp Asp Leu Ala Pro Arg Phe Lys Trp Leu Gly Leu Tyr
65 70 75 80
Thr Gln Arg Arg Gln Asp Leu Gly Gly Glu Leu Thr Gly Gln Leu Pro
85 90 95
Asp Ala Glu Leu Gln Asp Arg Tyr Phe Met Met Arg Val Arg Phe Glu
100 105 110
Gly Gly Gln Val Thr Pro Ala Arg Leu Arg Ala Val Gly Glu Ile Ser
115 120 125
Arg Asp Tyr Ala Arg Ser Thr Ala Asp Phe Thr Asp Arg Gln Asn Ile
130 135 140
Gln Leu His Trp Leu Arg Ile Glu Asp Ile Pro Ala Ile Trp Glu Lys
145 150 155 160
Leu Asp Ser Val Gly Leu Ser Pro Leu Leu Gly Cys Gly Asp Val Pro
165 170 175
Arg Ile Ile Leu Gly Ser Pro Val Ala Gly Val Ala Ala Glu Glu Ile
180 185 190
Ile Asp Ala Thr Pro Ala Ile Glu Arg Ile Gln Arg Glu His Leu Pro
195 200 205
Asn Pro Glu Phe His Asn Leu Pro Arg Lys Phe Lys Ser Ala Ile Ser
210 215 220
Gly His Ser Arg Gln Asp Val Thr His Glu Ile Gln Asp Val Ala Phe
225 230 235 240
Ile Gly Ser Val His Pro Glu His Gly Pro Gly Phe Glu Cys Phe Val
245 250 255
Gly Gly Gly Leu Ser Thr Asn Pro Met Leu Ala Gln Ser Leu Gly Ala
260 265 270
Trp Ile Pro Leu Glu Asp Val Pro Asp Val Trp Ala Gly Val Ala Arg
275 280 285
Ile Phe Arg Asp Tyr Gly Tyr Arg Arg Leu Arg Asn Arg Ala Arg Leu
290 295 300
Lys Phe Leu Val Ala Glu Trp Gly Ile Glu Lys Phe Arg Asp Val Leu
305 310 315 320
Glu Thr Glu Tyr Leu Gly Arg Pro Leu Ile Asp Gly Pro Arg Gln Pro
325 330 335
Thr Asn Pro Gly Tyr Arg Asp His Ile Gly Ile His Pro Gln Lys Asp
340 345 350
Gly Arg Phe Tyr Leu Gly Val Lys Pro Thr Val Gly His Thr Thr Gly
355 360 365
Glu Gln Leu Ile Ala Ile Ala Glu Val Ala Glu Arg Phe Gly Ile Glu
370 375 380
Arg Ile Arg Thr Thr Ser Glu Lys Glu Leu Leu Phe Leu Asp Val Glu
385 390 395 400
Arg Thr Asp Leu Thr Ala Leu Ala Ala Ala Leu Asp Glu Thr Gly Leu
405 410 415
Tyr Ser Lys Pro Ser Glu Trp Arg Arg Asn Ile Ile Ser Cys Thr Gly
420 425 430
Leu Glu Phe Cys Lys Leu Ala His Ala Thr Thr Lys Ser Arg Ala Val
435 440 445
Ala Leu Val Asp Glu Leu Glu Glu Arg Val Gly Asp Ile Asp Val Pro
450 455 460
Val Arg Ile Ala Val Asn Gly Cys Pro Asn Ser Cys Ala Arg Thr Gln
465 470 475 480
Val Ala Asp Ile Gly Phe Lys Gly Gln Thr Val Thr Asp Ala Asp Gly
485 490 495
Asn Arg Val Glu Gly Phe Gln Val His Leu Gly Gly Ser Met Ser Leu
500 505 510
Asn Ala Asn Phe Gly Arg Lys Leu Arg Gly His Lys Val Leu Ala Asp
515 520 525
Asp Ile Gly Asp Tyr Val Thr Arg Val Val Thr His Phe Arg Asp Gln
530 535 540
Arg Arg Pro Glu Glu Thr Phe Gln Glu Trp Val Gln Arg Ala Ala Glu
545 550 555 560
Glu Asp Leu Gln
<210> 79
<211> 1989
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1689)
<223> ген cysJ
<400> 79
acgtggattc ggcgccgggc gccgccacgg agcccctgcc ggggcgtctg cagtggatta 60
gagcagacag atcagttcat ggcaatggtg gagagatgta gatacgtcat gattttgggc 120
aactttcccg atcatggctg tacgtagtgt ttatatccga catacattca ctgaattgaa 180
ttgggggcgt cgaagagcga tgcccggacc ctgaattgaa gtcgcctttt cgcttgcatt 240
accgcccagg ggtgctactt tcacagacca gtcagtctag tgatttcccg aaaggaaccc 300
atg agc cag ccc acc ctc cgc atc gcc gtc atc ggc gcc ggc ccc gcc 348
Met Ser Gln Pro Thr Leu Arg Ile Ala Val Ile Gly Ala Gly Pro Ala
1 5 10 15
ggc atc tac gcc tcc gac ctc ctg gtg aag aac gcc gcc tcc atc ggc 396
Gly Ile Tyr Ala Ser Asp Leu Leu Val Lys Asn Ala Ala Ser Ile Gly
20 25 30
cgt gag gtc cac gtc gac ctc atc gag aag ctg ccc gcc ccc ttc ggg 444
Arg Glu Val His Val Asp Leu Ile Glu Lys Leu Pro Ala Pro Phe Gly
35 40 45
ctc atc cgc tac ggc gtc gcc ccc gac cac ccg cgc atc aag ggc atc 492
Leu Ile Arg Tyr Gly Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile
50 55 60
atc acc tcc ctg cac cgc gtc ctc gac cgc ccc gag atc cgc ctg ctc 540
Ile Thr Ser Leu His Arg Val Leu Asp Arg Pro Glu Ile Arg Leu Leu
65 70 75 80
ggc aac atc gag atc ggc cgc gac gtc acc gtc gag gag ctg cgc gaa 588
Gly Asn Ile Glu Ile Gly Arg Asp Val Thr Val Glu Glu Leu Arg Glu
85 90 95
cac tac gac gcc gtc atc ttc gcc acc ggc gcc atc gcc gac cgc gac 636
His Tyr Asp Ala Val Ile Phe Ala Thr Gly Ala Ile Ala Asp Arg Asp
100 105 110
ctg gac atc ccg ggc gtc gac gcg cag ggc tcc ttc ggc gcc gcc gac 684
Leu Asp Ile Pro Gly Val Asp Ala Gln Gly Ser Phe Gly Ala Ala Asp
115 120 125
ttc gtc ggc ttc tac gac ggc aac ccc gag ttc ccc cgc acc tgg gac 732
Phe Val Gly Phe Tyr Asp Gly Asn Pro Glu Phe Pro Arg Thr Trp Asp
130 135 140
ctg tcc gcg cag tcc gtc gcc gtc atc ggc gtg ggc aac gtg ggc ctc 780
Leu Ser Ala Gln Ser Val Ala Val Ile Gly Val Gly Asn Val Gly Leu
145 150 155 160
gac gtc gcg cgc gtc ctg tcc aag acc gcc gac gaa ctc aac gtc acc 828
Asp Val Ala Arg Val Leu Ser Lys Thr Ala Asp Glu Leu Asn Val Thr
165 170 175
gag atc ccc gac aac gtc tac gag acc ctc cgc acc aac cag gcc acc 876
Glu Ile Pro Asp Asn Val Tyr Glu Thr Leu Arg Thr Asn Gln Ala Thr
180 185 190
gag gtc cac gtc ttc ggc cgc cgc ggc ccc gcg cag gcc aag ttc acc 924
Glu Val His Val Phe Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr
195 200 205
ccg cag gaa ctc aag gaa ctc gac cac tcc ccc acc gtc aac gtc gtc 972
Pro Gln Glu Leu Lys Glu Leu Asp His Ser Pro Thr Val Asn Val Val
210 215 220
gtc gac ccc gag gac atc gac tac gac gac gcc agc atc gag gcc cgc 1020
Val Asp Pro Glu Asp Ile Asp Tyr Asp Asp Ala Ser Ile Glu Ala Arg
225 230 235 240
agc agc tcc aag tcg cag gac ctc gtg tgc cag acc ctc gag ggc tac 1068
Ser Ser Ser Lys Ser Gln Asp Leu Val Cys Gln Thr Leu Glu Gly Tyr
245 250 255
gcc atc cgc gaa ccc aag gac gcc ccg cac acc ctg cag atc cac ctc 1116
Ala Ile Arg Glu Pro Lys Asp Ala Pro His Thr Leu Gln Ile His Leu
260 265 270
ttc gag tct ccc acc gag atc ctc acc cgc gac ggc cgt gtc gtc ggc 1164
Phe Glu Ser Pro Thr Glu Ile Leu Thr Arg Asp Gly Arg Val Val Gly
275 280 285
ctg cgc acc gaa cgc acc gaa ctc gac ggc acc ggc cac gtc cgc ggc 1212
Leu Arg Thr Glu Arg Thr Glu Leu Asp Gly Thr Gly His Val Arg Gly
290 295 300
acc ggc acc ttc acc gac tgg ccc gtc cag gcc gtc tac cgc gcc gtc 1260
Thr Gly Thr Phe Thr Asp Trp Pro Val Gln Ala Val Tyr Arg Ala Val
305 310 315 320
ggc tac cgc tcc gag ccg gtg ccg ggc gtg ccc ttc aac cat gtc gag 1308
Gly Tyr Arg Ser Glu Pro Val Pro Gly Val Pro Phe Asn His Val Glu
325 330 335
cat gtg atc ccg aac gac ggc ggc cac gtc ctc gac ccc gcc ggc acc 1356
His Val Ile Pro Asn Asp Gly Gly His Val Leu Asp Pro Ala Gly Thr
340 345 350
ccc gtc ccc ggc ctc tac gcc acc ggc tgg atc aag cgc ggc ccc gtc 1404
Pro Val Pro Gly Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Val
355 360 365
gga ctc atc ggc aac acc aag tcc gac gcc aag gag acc acc gac ctc 1452
Gly Leu Ile Gly Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Asp Leu
370 375 380
ctg ctc gcc gac gcc gcc gcc gga ctt ctc gac gcc ccc ctc cac ccc 1500
Leu Leu Ala Asp Ala Ala Ala Gly Leu Leu Asp Ala Pro Leu His Pro
385 390 395 400
gcc gac gcc ttc ctc cag ctt ctc gac gcc cgc ggc atc ccc gtc acc 1548
Ala Asp Ala Phe Leu Gln Leu Leu Asp Ala Arg Gly Ile Pro Val Thr
405 410 415
acc tgg gag ggc tgg tac cgg ctc gac gcc gcc gag cgc gcc ctc ggc 1596
Thr Trp Glu Gly Trp Tyr Arg Leu Asp Ala Ala Glu Arg Ala Leu Gly
420 425 430
gag gcc tcc ggc gtg gag ggc aag gag cgc cgg aag atc gtc gaa tgg 1644
Glu Ala Ser Gly Val Glu Gly Lys Glu Arg Arg Lys Ile Val Glu Trp
435 440 445
gag gag atg gtc agc cac gca cac atc acc ccg gcc tat gtg tga 1689
Glu Glu Met Val Ser His Ala His Ile Thr Pro Ala Tyr Val
450 455 460
tttctcaggc tcagggccgg cagctgagac tgggcagcgc tatgccacgg gccatgacac 1749
gccgggccgg ataaaaattc ctgaccctga gtcgatctaa ctgcggtaac ggtccggtag 1809
gcgaattgtt accgatcagc aaccgtccaa attcataccc actaatgtga tcactgttac 1869
tctcaagagt ggaaagtaca actgacaatt cttaaggaga aacatcatgc gcggtagcgg 1929
actcatctgg accatcgtcg gcatcctggc catcatcgcc ctagtcatct ggatcatgtc 1989
<210> 80
<211> 462
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 80
Met Ser Gln Pro Thr Leu Arg Ile Ala Val Ile Gly Ala Gly Pro Ala
1 5 10 15
Gly Ile Tyr Ala Ser Asp Leu Leu Val Lys Asn Ala Ala Ser Ile Gly
20 25 30
Arg Glu Val His Val Asp Leu Ile Glu Lys Leu Pro Ala Pro Phe Gly
35 40 45
Leu Ile Arg Tyr Gly Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile
50 55 60
Ile Thr Ser Leu His Arg Val Leu Asp Arg Pro Glu Ile Arg Leu Leu
65 70 75 80
Gly Asn Ile Glu Ile Gly Arg Asp Val Thr Val Glu Glu Leu Arg Glu
85 90 95
His Tyr Asp Ala Val Ile Phe Ala Thr Gly Ala Ile Ala Asp Arg Asp
100 105 110
Leu Asp Ile Pro Gly Val Asp Ala Gln Gly Ser Phe Gly Ala Ala Asp
115 120 125
Phe Val Gly Phe Tyr Asp Gly Asn Pro Glu Phe Pro Arg Thr Trp Asp
130 135 140
Leu Ser Ala Gln Ser Val Ala Val Ile Gly Val Gly Asn Val Gly Leu
145 150 155 160
Asp Val Ala Arg Val Leu Ser Lys Thr Ala Asp Glu Leu Asn Val Thr
165 170 175
Glu Ile Pro Asp Asn Val Tyr Glu Thr Leu Arg Thr Asn Gln Ala Thr
180 185 190
Glu Val His Val Phe Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr
195 200 205
Pro Gln Glu Leu Lys Glu Leu Asp His Ser Pro Thr Val Asn Val Val
210 215 220
Val Asp Pro Glu Asp Ile Asp Tyr Asp Asp Ala Ser Ile Glu Ala Arg
225 230 235 240
Ser Ser Ser Lys Ser Gln Asp Leu Val Cys Gln Thr Leu Glu Gly Tyr
245 250 255
Ala Ile Arg Glu Pro Lys Asp Ala Pro His Thr Leu Gln Ile His Leu
260 265 270
Phe Glu Ser Pro Thr Glu Ile Leu Thr Arg Asp Gly Arg Val Val Gly
275 280 285
Leu Arg Thr Glu Arg Thr Glu Leu Asp Gly Thr Gly His Val Arg Gly
290 295 300
Thr Gly Thr Phe Thr Asp Trp Pro Val Gln Ala Val Tyr Arg Ala Val
305 310 315 320
Gly Tyr Arg Ser Glu Pro Val Pro Gly Val Pro Phe Asn His Val Glu
325 330 335
His Val Ile Pro Asn Asp Gly Gly His Val Leu Asp Pro Ala Gly Thr
340 345 350
Pro Val Pro Gly Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Val
355 360 365
Gly Leu Ile Gly Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Asp Leu
370 375 380
Leu Leu Ala Asp Ala Ala Ala Gly Leu Leu Asp Ala Pro Leu His Pro
385 390 395 400
Ala Asp Ala Phe Leu Gln Leu Leu Asp Ala Arg Gly Ile Pro Val Thr
405 410 415
Thr Trp Glu Gly Trp Tyr Arg Leu Asp Ala Ala Glu Arg Ala Leu Gly
420 425 430
Glu Ala Ser Gly Val Glu Gly Lys Glu Arg Arg Lys Ile Val Glu Trp
435 440 445
Glu Glu Met Val Ser His Ala His Ile Thr Pro Ala Tyr Val
450 455 460
<210> 81
<211> 1869
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1569)
<223> ген cysN
<400> 81
cgcgctgccg gagatctact tcgcgcatga gcgggccgtc ttcgagcggg acggcatgtg 60
gctgaccgcc ggcgagtggg gtgggccccg cgagggcgag accctcgagg tccgccgcgt 120
gcgctaccgc accgtcggcg acatgtcctg caccggtgcg gtgctctccg acgccaccac 180
ggtcgacgac gtcatcgccg agatcgccac ctccaccctc accgagcgcg gggccacccg 240
cgccgacgac cgcctcagtg aatccgccat ggaagaccgc aagaaggaag gctacttctg 300
atg acc acc acg ctc ctg cgc acc cgc gag acg ctg cgc ctg tgc acc 348
Met Thr Thr Thr Leu Leu Arg Thr Arg Glu Thr Leu Arg Leu Cys Thr
1 5 10 15
gcc ggc tcc gtc gac gac ggc aag tcc acc ctc gtg ggc cgc ctg ctc 396
Ala Gly Ser Val Asp Asp Gly Lys Ser Thr Leu Val Gly Arg Leu Leu
20 25 30
cac gac acc cgc tcc gtc ctc gcc gac cag ctc gcc gcc gtc gag cgc 444
His Asp Thr Arg Ser Val Leu Ala Asp Gln Leu Ala Ala Val Glu Arg
35 40 45
acc tcc gcc gac cgc ggt ttc cac ggc ggc ctg gac ctc tca ctg ctc 492
Thr Ser Ala Asp Arg Gly Phe His Gly Gly Leu Asp Leu Ser Leu Leu
50 55 60
gtc gac ggc ctc cgc gcc gaa cgc gag cag ggc atc acc atc gac gtg 540
Val Asp Gly Leu Arg Ala Glu Arg Glu Gln Gly Ile Thr Ile Asp Val
65 70 75 80
gcc tac cgc tac ttc gcc acc gac cgc cgc acc ttc atc ctc gcc gac 588
Ala Tyr Arg Tyr Phe Ala Thr Asp Arg Arg Thr Phe Ile Leu Ala Asp
85 90 95
acc ccc ggc cac gtg cag tac acg cgc aac acc gtc acc ggc gtg tcc 636
Thr Pro Gly His Val Gln Tyr Thr Arg Asn Thr Val Thr Gly Val Ser
100 105 110
acg tcg cag gtg gtc gtc ctg ctt gtc gac gcc cgc cac ggc gtc gtc 684
Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg His Gly Val Val
115 120 125
gag cag acc cgc cgc cac ctc acc gtc gcg gcg ctc ctc ggg gtg cgc 732
Glu Gln Thr Arg Arg His Leu Thr Val Ala Ala Leu Leu Gly Val Arg
130 135 140
aca gtg ctc ctc gcg gtg aat aag atc gac ctc gtt gac tgg tcc gag 780
Thr Val Leu Leu Ala Val Asn Lys Ile Asp Leu Val Asp Trp Ser Glu
145 150 155 160
gcc gtc ttc cgg gac atc gag gcg gag ttc acg tcg ctg gcc acc gac 828
Ala Val Phe Arg Asp Ile Glu Ala Glu Phe Thr Ser Leu Ala Thr Asp
165 170 175
gtc ggc gtc gac gac gcg cac gtc atc ccc gtc tcc gcg ctg ctg ggc 876
Val Gly Val Asp Asp Ala His Val Ile Pro Val Ser Ala Leu Leu Gly
180 185 190
gac aac gtc gtc gaa ccc tcg gcg aac ctc ccc tgg tac gag ggg ccg 924
Asp Asn Val Val Glu Pro Ser Ala Asn Leu Pro Trp Tyr Glu Gly Pro
195 200 205
acg gtg ctg gaa ctg ctc gag agc gtc gag gtc cac cgc ggc cgc gcc 972
Thr Val Leu Glu Leu Leu Glu Ser Val Glu Val His Arg Gly Arg Ala
210 215 220
cac gcc ctc ggc ttc cgc ctg ccg atc cag tac gtc atc cgc gag cac 1020
His Ala Leu Gly Phe Arg Leu Pro Ile Gln Tyr Val Ile Arg Glu His
225 230 235 240
tcc tcc gac tac cgc ggc tac gcc ggg cgt gtc gac gcc ggc gtg ctc 1068
Ser Ser Asp Tyr Arg Gly Tyr Ala Gly Arg Val Asp Ala Gly Val Leu
245 250 255
acc gtc ggc gac acc gtc cac ctc ccc gag ggg cgc acc agc acc gtc 1116
Thr Val Gly Asp Thr Val His Leu Pro Glu Gly Arg Thr Ser Thr Val
260 265 270
acc cac atc gac acc gcc gac ggc gag gtc cgc tcc gcc cac gcc ggt 1164
Thr His Ile Asp Thr Ala Asp Gly Glu Val Arg Ser Ala His Ala Gly
275 280 285
gac tcc gtg gtc ctg cgc ctg gcc gac gac atc gac ctc atc cgc ggc 1212
Asp Ser Val Val Leu Arg Leu Ala Asp Asp Ile Asp Leu Ile Arg Gly
290 295 300
gac ctc atc gcc ggt gag gac cgc ccg gag ccg acc cgc tcc ttc gcc 1260
Asp Leu Ile Ala Gly Glu Asp Arg Pro Glu Pro Thr Arg Ser Phe Ala
305 310 315 320
gcc aca ctc gtc gga ctc ggc gac cgc acc ctg cgc ccc ggc tcc ccc 1308
Ala Thr Leu Val Gly Leu Gly Asp Arg Thr Leu Arg Pro Gly Ser Pro
325 330 335
gtg aag gtc cgc tac ggc acc tca ctc gtg cgg gga cgc gtc gcg gcg 1356
Val Lys Val Arg Tyr Gly Thr Ser Leu Val Arg Gly Arg Val Ala Ala
340 345 350
atc gag cgg ctg ctc gac atc gac ggc cgc acc gac cac ctc tcc ccg 1404
Ile Glu Arg Leu Leu Asp Ile Asp Gly Arg Thr Asp His Leu Ser Pro
355 360 365
gac gac ctc ggc ctc aac gac atc gcc cac gtg cgt atc gac gtc gcc 1452
Asp Asp Leu Gly Leu Asn Asp Ile Ala His Val Arg Ile Asp Val Ala
370 375 380
acc gag ctc ccc gtc gag ccc tac gcc gcc cgc ggc gcc gtc ggc tcc 1500
Thr Glu Leu Pro Val Glu Pro Tyr Ala Ala Arg Gly Ala Val Gly Ser
385 390 395 400
ttc ctg ctc atc gac cag gcc agc ggc gac acc ctc gcc gcc ggg ctc 1548
Phe Leu Leu Ile Asp Gln Ala Ser Gly Asp Thr Leu Ala Ala Gly Leu
405 410 415
gtc ggc cac cgg ctc cgc tga cccgtgaccg ccctgctcac cctctcccac 1599
Val Gly His Arg Leu Arg
420
gggtcccgcc acccgcgcgc cgccgccggc gtcgaccgcc tcacccgggc ggccggggag 1659
gtgctgggcg tcaccgcccg cgccgcccac ctcgagttcg acaccccctc cctcatcgac 1719
gccgcccgcg acctcgtcgc ccgcggccac gaccacgccg tggtcgtccc cctgctgttc 1779
accgacgcct tccacgcgcg tcacgacgtc cccgccgccc tcgacgcggc ccgccgcgcc 1839
accggcctgc ggctcacgcc cgcccggggc 1869
<210> 82
<211> 422
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 82
Met Thr Thr Thr Leu Leu Arg Thr Arg Glu Thr Leu Arg Leu Cys Thr
1 5 10 15
Ala Gly Ser Val Asp Asp Gly Lys Ser Thr Leu Val Gly Arg Leu Leu
20 25 30
His Asp Thr Arg Ser Val Leu Ala Asp Gln Leu Ala Ala Val Glu Arg
35 40 45
Thr Ser Ala Asp Arg Gly Phe His Gly Gly Leu Asp Leu Ser Leu Leu
50 55 60
Val Asp Gly Leu Arg Ala Glu Arg Glu Gln Gly Ile Thr Ile Asp Val
65 70 75 80
Ala Tyr Arg Tyr Phe Ala Thr Asp Arg Arg Thr Phe Ile Leu Ala Asp
85 90 95
Thr Pro Gly His Val Gln Tyr Thr Arg Asn Thr Val Thr Gly Val Ser
100 105 110
Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg His Gly Val Val
115 120 125
Glu Gln Thr Arg Arg His Leu Thr Val Ala Ala Leu Leu Gly Val Arg
130 135 140
Thr Val Leu Leu Ala Val Asn Lys Ile Asp Leu Val Asp Trp Ser Glu
145 150 155 160
Ala Val Phe Arg Asp Ile Glu Ala Glu Phe Thr Ser Leu Ala Thr Asp
165 170 175
Val Gly Val Asp Asp Ala His Val Ile Pro Val Ser Ala Leu Leu Gly
180 185 190
Asp Asn Val Val Glu Pro Ser Ala Asn Leu Pro Trp Tyr Glu Gly Pro
195 200 205
Thr Val Leu Glu Leu Leu Glu Ser Val Glu Val His Arg Gly Arg Ala
210 215 220
His Ala Leu Gly Phe Arg Leu Pro Ile Gln Tyr Val Ile Arg Glu His
225 230 235 240
Ser Ser Asp Tyr Arg Gly Tyr Ala Gly Arg Val Asp Ala Gly Val Leu
245 250 255
Thr Val Gly Asp Thr Val His Leu Pro Glu Gly Arg Thr Ser Thr Val
260 265 270
Thr His Ile Asp Thr Ala Asp Gly Glu Val Arg Ser Ala His Ala Gly
275 280 285
Asp Ser Val Val Leu Arg Leu Ala Asp Asp Ile Asp Leu Ile Arg Gly
290 295 300
Asp Leu Ile Ala Gly Glu Asp Arg Pro Glu Pro Thr Arg Ser Phe Ala
305 310 315 320
Ala Thr Leu Val Gly Leu Gly Asp Arg Thr Leu Arg Pro Gly Ser Pro
325 330 335
Val Lys Val Arg Tyr Gly Thr Ser Leu Val Arg Gly Arg Val Ala Ala
340 345 350
Ile Glu Arg Leu Leu Asp Ile Asp Gly Arg Thr Asp His Leu Ser Pro
355 360 365
Asp Asp Leu Gly Leu Asn Asp Ile Ala His Val Arg Ile Asp Val Ala
370 375 380
Thr Glu Leu Pro Val Glu Pro Tyr Ala Ala Arg Gly Ala Val Gly Ser
385 390 395 400
Phe Leu Leu Ile Asp Gln Ala Ser Gly Asp Thr Leu Ala Ala Gly Leu
405 410 415
Val Gly His Arg Leu Arg
420
<210> 83
<211> 1248
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(948)
<223> ген cysY
<400> 83
ggactcggcg accgcaccct gcgccccggc tcccccgtga aggtccgcta cggcacctca 60
ctcgtgcggg gacgcgtcgc ggcgatcgag cggctgctcg acatcgacgg ccgcaccgac 120
cacctctccc cggacgacct cggcctcaac gacatcgccc acgtgcgtat cgacgtcgcc 180
accgagctcc ccgtcgagcc ctacgccgcc cgcggcgccg tcggctcctt cctgctcatc 240
gaccaggcca gcggcgacac cctcgccgcc gggctcgtcg gccaccggct ccgctgaccc 300
gtg acc gcc ctg ctc acc ctc tcc cac ggg tcc cgc cac ccg cgc gcc 348
Val Thr Ala Leu Leu Thr Leu Ser His Gly Ser Arg His Pro Arg Ala
1 5 10 15
gcc gcc ggc gtc gac cgc ctc acc cgg gcg gcc ggg gag gtg ctg ggc 396
Ala Ala Gly Val Asp Arg Leu Thr Arg Ala Ala Gly Glu Val Leu Gly
20 25 30
gtc acc gcc cgc gcc gcc cac ctc gag ttc gac acc ccc tcc ctc atc 444
Val Thr Ala Arg Ala Ala His Leu Glu Phe Asp Thr Pro Ser Leu Ile
35 40 45
gac gcc gcc cgc gac ctc gtc gcc cgc ggc cac gac cac gcc gtg gtc 492
Asp Ala Ala Arg Asp Leu Val Ala Arg Gly His Asp His Ala Val Val
50 55 60
gtc ccc ctg ctg ttc acc gac gcc ttc cac gcg cgt cac gac gtc ccc 540
Val Pro Leu Leu Phe Thr Asp Ala Phe His Ala Arg His Asp Val Pro
65 70 75 80
gcc gcc ctc gac gcg gcc cgc cgc gcc acc ggc ctg cgg ctc acg ccc 588
Ala Ala Leu Asp Ala Ala Arg Arg Ala Thr Gly Leu Arg Leu Thr Pro
85 90 95
gcc cgg ggc ctc ggc acc ggc ccc gac atc gcc gac gtc ctt gcc cgc 636
Ala Arg Gly Leu Gly Thr Gly Pro Asp Ile Ala Asp Val Leu Ala Arg
100 105 110
cac acc cgc ccc cgc aac ccg gac ctc gtc ctc ggg cac gtc gga tcc 684
His Thr Arg Pro Arg Asn Pro Asp Leu Val Leu Gly His Val Gly Ser
115 120 125
tcc tcc gtc gca gcc gag gcc gcc gtc cgg tcg ctg gcc gac ctc ctc 732
Ser Ser Val Ala Ala Glu Ala Ala Val Arg Ser Leu Ala Asp Leu Leu
130 135 140
gcc gcc cgc ctc ggg cgg gac gtc gcg gtg gtg cgg gcg acg ggg gag 780
Ala Ala Arg Leu Gly Arg Asp Val Ala Val Val Arg Ala Thr Gly Glu
145 150 155 160
aag gcg gcg tcg ata agc agc ggt gcc cac ctc gtc ccc ctc ttc gtc 828
Lys Ala Ala Ser Ile Ser Ser Gly Ala His Leu Val Pro Leu Phe Val
165 170 175
acc gac gcc ctg ctg ctc gac cgc ctg cgc gcc gcc caa ccc cac gcg 876
Thr Asp Ala Leu Leu Leu Asp Arg Leu Arg Ala Ala Gln Pro His Ala
180 185 190
acc tcc gac cgc ccg ctc acc gac gcc ctc cgc gac gtc gtg gcg gcg 924
Thr Ser Asp Arg Pro Leu Thr Asp Ala Leu Arg Asp Val Val Ala Ala
195 200 205
cgg tac cgg gcc gcc ctc acc tag gaacccacca tgaacacact cctgctcatc 978
Arg Tyr Arg Ala Ala Leu Thr
210 215
gccctcgcgg ggctcgccgc ccagctcgtc gacggcggcc tcggcatggg cttcggcgcc 1038
acctccacca cgatcctcat cgtgctcgcc ggactcggcc ccgccacagc cagcgccgtc 1098
gtccacaccg cggaactggg caccaccctc gtctccggcg tctcgcactg gcgtttcgga 1158
aacgtcgact ggcaggtcgt ccgccgcctc gccatccccg gtgccgtggg cgccttcgcc 1218
ggggcaaccg tgctctccca cttatcgacg 1248
<210> 84
<211> 215
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 84
Val Thr Ala Leu Leu Thr Leu Ser His Gly Ser Arg His Pro Arg Ala
1 5 10 15
Ala Ala Gly Val Asp Arg Leu Thr Arg Ala Ala Gly Glu Val Leu Gly
20 25 30
Val Thr Ala Arg Ala Ala His Leu Glu Phe Asp Thr Pro Ser Leu Ile
35 40 45
Asp Ala Ala Arg Asp Leu Val Ala Arg Gly His Asp His Ala Val Val
50 55 60
Val Pro Leu Leu Phe Thr Asp Ala Phe His Ala Arg His Asp Val Pro
65 70 75 80
Ala Ala Leu Asp Ala Ala Arg Arg Ala Thr Gly Leu Arg Leu Thr Pro
85 90 95
Ala Arg Gly Leu Gly Thr Gly Pro Asp Ile Ala Asp Val Leu Ala Arg
100 105 110
His Thr Arg Pro Arg Asn Pro Asp Leu Val Leu Gly His Val Gly Ser
115 120 125
Ser Ser Val Ala Ala Glu Ala Ala Val Arg Ser Leu Ala Asp Leu Leu
130 135 140
Ala Ala Arg Leu Gly Arg Asp Val Ala Val Val Arg Ala Thr Gly Glu
145 150 155 160
Lys Ala Ala Ser Ile Ser Ser Gly Ala His Leu Val Pro Leu Phe Val
165 170 175
Thr Asp Ala Leu Leu Leu Asp Arg Leu Arg Ala Ala Gln Pro His Ala
180 185 190
Thr Ser Asp Arg Pro Leu Thr Asp Ala Leu Arg Asp Val Val Ala Ala
195 200 205
Arg Tyr Arg Ala Ala Leu Thr
210 215
<210> 85
<211> 1515
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1215)
<223> ген cysZ
<400> 85
gacctcgtcc tcgggcacgt cggatcctcc tccgtcgcag ccgaggccgc cgtccggtcg 60
ctggccgacc tcctcgccgc ccgcctcggg cgggacgtcg cggtggtgcg ggcgacgggg 120
gagaaggcgg cgtcgataag cagcggtgcc cacctcgtcc ccctcttcgt caccgacgcc 180
ctgctgctcg accgcctgcg cgccgcccaa ccccacgcga cctccgaccg cccgctcacc 240
gacgccctcc gcgacgtcgt ggcggcgcgg taccgggccg ccctcaccta ggaacccacc 300
atg aac aca ctc ctg ctc atc gcc ctc gcg ggg ctc gcc gcc cag ctc 348
Met Asn Thr Leu Leu Leu Ile Ala Leu Ala Gly Leu Ala Ala Gln Leu
1 5 10 15
gtc gac ggc ggc ctc ggc atg ggc ttc ggc gcc acc tcc acc acg atc 396
Val Asp Gly Gly Leu Gly Met Gly Phe Gly Ala Thr Ser Thr Thr Ile
20 25 30
ctc atc gtg ctc gcc gga ctc ggc ccc gcc aca gcc agc gcc gtc gtc 444
Leu Ile Val Leu Ala Gly Leu Gly Pro Ala Thr Ala Ser Ala Val Val
35 40 45
cac acc gcg gaa ctg ggc acc acc ctc gtc tcc ggc gtc tcg cac tgg 492
His Thr Ala Glu Leu Gly Thr Thr Leu Val Ser Gly Val Ser His Trp
50 55 60
cgt ttc gga aac gtc gac tgg cag gtc gtc cgc cgc ctc gcc atc ccc 540
Arg Phe Gly Asn Val Asp Trp Gln Val Val Arg Arg Leu Ala Ile Pro
65 70 75 80
ggt gcc gtg ggc gcc ttc gcc ggg gca acc gtg ctc tcc cac tta tcg 588
Gly Ala Val Gly Ala Phe Ala Gly Ala Thr Val Leu Ser His Leu Ser
85 90 95
acg cag gcc gcc gcc ccc gtc acc gcc acg atc ctc acc ctc atc ggc 636
Thr Gln Ala Ala Ala Pro Val Thr Ala Thr Ile Leu Thr Leu Ile Gly
100 105 110
gcc aac ctc gtg tgg cgc ttc tcc cgc gga cgc atc cac ccg cgc cgc 684
Ala Asn Leu Val Trp Arg Phe Ser Arg Gly Arg Ile His Pro Arg Arg
115 120 125
gcc ggg aag ggg cac tcg cgc ggc ttc ctc ggc ggg ctc ggc ctg ttc 732
Ala Gly Lys Gly His Ser Arg Gly Phe Leu Gly Gly Leu Gly Leu Phe
130 135 140
ggc ggc ttc atc gac gcc tcc gga ggc ggc ggc tgg ggc ccg gtg acc 780
Gly Gly Phe Ile Asp Ala Ser Gly Gly Gly Gly Trp Gly Pro Val Thr
145 150 155 160
acc tcg acc ctg atg acg ctg ggg cgg gcg gaa ccg cgc cgc atc gtc 828
Thr Ser Thr Leu Met Thr Leu Gly Arg Ala Glu Pro Arg Arg Ile Val
165 170 175
ggc acg gtc aac acc gcc gag ttc ctg gtc acc gcc gcg gcg acc gcc 876
Gly Thr Val Asn Thr Ala Glu Phe Leu Val Thr Ala Ala Ala Thr Ala
180 185 190
ggc ttc gtc atc ggc ctg tgg gac gac ctg gtg gcc aac ctc gcg gcc 924
Gly Phe Val Ile Gly Leu Trp Asp Asp Leu Val Ala Asn Leu Ala Ala
195 200 205
gtg ctc gcg ctg ctc atc ggc ggt gtc atc gcc gcg ccg gtc gcc gcg 972
Val Leu Ala Leu Leu Ile Gly Gly Val Ile Ala Ala Pro Val Ala Ala
210 215 220
tgg ctg gtc acc cgc ctc aac ccg gcc gcc ctc ggc ggc gtc gtg ggc 1020
Trp Leu Val Thr Arg Leu Asn Pro Ala Ala Leu Gly Gly Val Val Gly
225 230 235 240
acc ctg ctg gtc gcg ctg aac ctc ccc aag gtg ctg ccc ttc gcc gga 1068
Thr Leu Leu Val Ala Leu Asn Leu Pro Lys Val Leu Pro Phe Ala Gly
245 250 255
gga ccg gtg ctg ctg gcc gtc atc gtc gtc ggc ctg ggc ttc tcg tgg 1116
Gly Pro Val Leu Leu Ala Val Ile Val Val Gly Leu Gly Phe Ser Trp
260 265 270
gtc ggc tgg cga cgc tcc ctc cgt gcc cgc gcc gcc gcc ggg cag ccc 1164
Val Gly Trp Arg Arg Ser Leu Arg Ala Arg Ala Ala Ala Gly Gln Pro
275 280 285
gtc acc gga acg acg agc acc ccc acc gga cag tcg gtg ggg gtg cgg 1212
Val Thr Gly Thr Thr Ser Thr Pro Thr Gly Gln Ser Val Gly Val Arg
290 295 300
tag ggcgggggct acagggcggc gacgtcgccg atgacgtaca cggccgggga 1265
cccgatgcgg ttcccggcca tcgtcgtggc cagggttccc aacgtgcacc ggaaggaacg 1325
ctgcccagtg gtggtgccct cctggatgac ggcggccggg gtgtgggggt cgaggccggc 1385
gtcgacaagc gtggcggtga tggcggcgac gttgcgcaca cccatgatca ccgagatcgt 1445
gccgcccgcc cgcgccagcg cggcccagtc gacgagcgag tccgggtgcc ccggcggcag 1505
atgcccggag 1515
<210> 86
<211> 304
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 86
Met Asn Thr Leu Leu Leu Ile Ala Leu Ala Gly Leu Ala Ala Gln Leu
1 5 10 15
Val Asp Gly Gly Leu Gly Met Gly Phe Gly Ala Thr Ser Thr Thr Ile
20 25 30
Leu Ile Val Leu Ala Gly Leu Gly Pro Ala Thr Ala Ser Ala Val Val
35 40 45
His Thr Ala Glu Leu Gly Thr Thr Leu Val Ser Gly Val Ser His Trp
50 55 60
Arg Phe Gly Asn Val Asp Trp Gln Val Val Arg Arg Leu Ala Ile Pro
65 70 75 80
Gly Ala Val Gly Ala Phe Ala Gly Ala Thr Val Leu Ser His Leu Ser
85 90 95
Thr Gln Ala Ala Ala Pro Val Thr Ala Thr Ile Leu Thr Leu Ile Gly
100 105 110
Ala Asn Leu Val Trp Arg Phe Ser Arg Gly Arg Ile His Pro Arg Arg
115 120 125
Ala Gly Lys Gly His Ser Arg Gly Phe Leu Gly Gly Leu Gly Leu Phe
130 135 140
Gly Gly Phe Ile Asp Ala Ser Gly Gly Gly Gly Trp Gly Pro Val Thr
145 150 155 160
Thr Ser Thr Leu Met Thr Leu Gly Arg Ala Glu Pro Arg Arg Ile Val
165 170 175
Gly Thr Val Asn Thr Ala Glu Phe Leu Val Thr Ala Ala Ala Thr Ala
180 185 190
Gly Phe Val Ile Gly Leu Trp Asp Asp Leu Val Ala Asn Leu Ala Ala
195 200 205
Val Leu Ala Leu Leu Ile Gly Gly Val Ile Ala Ala Pro Val Ala Ala
210 215 220
Trp Leu Val Thr Arg Leu Asn Pro Ala Ala Leu Gly Gly Val Val Gly
225 230 235 240
Thr Leu Leu Val Ala Leu Asn Leu Pro Lys Val Leu Pro Phe Ala Gly
245 250 255
Gly Pro Val Leu Leu Ala Val Ile Val Val Gly Leu Gly Phe Ser Trp
260 265 270
Val Gly Trp Arg Arg Ser Leu Arg Ala Arg Ala Ala Ala Gly Gln Pro
275 280 285
Val Thr Gly Thr Thr Ser Thr Pro Thr Gly Gln Ser Val Gly Val Arg
290 295 300
<210> 87
<211> 2916
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2616)
<223> ген metE
<400> 87
gactggaggc ggcaggcccg gagagcgagt accagacggg cctcgacctc acggccgagc 60
tcgcgcagac catcctggac gccggtgccg gggggatgca cgtgtacacg ttcaaccggc 120
cggacaccac caccgaactg ctggaccgca tcggcatcac ccccgaccct cgggttaggg 180
agtgaggcgg gagaccgccg ccgctgcatc tcatgttgat agcgacctgc tgcacgcacc 240
gggagccccc gaagcagacc aggttgtaca cccaagattt tccgaaaggc ctcccttttc 300
atg tct tcc acc gct ttc ccc acc ttc acc atc gag ggc tac ccg cgc 348
Met Ser Ser Thr Ala Phe Pro Thr Phe Thr Ile Glu Gly Tyr Pro Arg
1 5 10 15
gtc ggc gcc aac cgc gag ctc aag aag gcc ctc gag tcc ttc tgg gcc 396
Val Gly Ala Asn Arg Glu Leu Lys Lys Ala Leu Glu Ser Phe Trp Ala
20 25 30
ggc cgc atc gac gag gag acc ttc atc tcc tcc gcg cac tcc atc cgc 444
Gly Arg Ile Asp Glu Glu Thr Phe Ile Ser Ser Ala His Ser Ile Arg
35 40 45
ctg gag aac tac gcc cgt ctg cgt gac ctc ggc ctg gac cag gac tac 492
Leu Glu Asn Tyr Ala Arg Leu Arg Asp Leu Gly Leu Asp Gln Asp Tyr
50 55 60
gcc atc ccg gcc gac gtc gcc ctc tac gac cag gtg ctc gag acc ggc 540
Ala Ile Pro Ala Asp Val Ala Leu Tyr Asp Gln Val Leu Glu Thr Gly
65 70 75 80
gtc acc gtc ggc ctg atc ggt ggc gac gcc gcc gag gtc gac ctc acc 588
Val Thr Val Gly Leu Ile Gly Gly Asp Ala Ala Glu Val Asp Leu Thr
85 90 95
gag tac ttc gcc ctg gcc cgc ggc acc gcc gag cgt tcc ccg ctc gag 636
Glu Tyr Phe Ala Leu Ala Arg Gly Thr Ala Glu Arg Ser Pro Leu Glu
100 105 110
atg acc aag tgg ttc gac acc aac tac cac tac ctc gtc ccg gag gtc 684
Met Thr Lys Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Val
115 120 125
gcc gag gac acc gtc atc acc ccg cgc ccg cag cgc atc ctc gac atc 732
Ala Glu Asp Thr Val Ile Thr Pro Arg Pro Gln Arg Ile Leu Asp Ile
130 135 140
gtc gcc gag gcc aag gag gcc ggc cac acc gtc cgt ccg ttc ctg gtc 780
Val Ala Glu Ala Lys Glu Ala Gly His Thr Val Arg Pro Phe Leu Val
145 150 155 160
ggc ccg gtc acc ctg ctg gcc aag tcc aag gcc gcc gag aac gcc ggc 828
Gly Pro Val Thr Leu Leu Ala Lys Ser Lys Ala Ala Glu Asn Ala Gly
165 170 175
gag ggc ttc aac ccg ctg tcc cgc ctc gag gac ctc acc gcc gcc tac 876
Glu Gly Phe Asn Pro Leu Ser Arg Leu Glu Asp Leu Thr Ala Ala Tyr
180 185 190
gcc cag gtc ctc gcc gcc ctc aag gag gcc ggc gtg gag tgg gtc cag 924
Ala Gln Val Leu Ala Ala Leu Lys Glu Ala Gly Val Glu Trp Val Gln
195 200 205
ctc gcc gag ccg gcc ctg gtc gcc gac ctg acc atc cgt tcc gac gcc 972
Leu Ala Glu Pro Ala Leu Val Ala Asp Leu Thr Ile Arg Ser Asp Ala
210 215 220
gac ctg gcc gcc gac gcg aag gcc acc tac gag gcg ctc ctc ggc tcc 1020
Asp Leu Ala Ala Asp Ala Lys Ala Thr Tyr Glu Ala Leu Leu Gly Ser
225 230 235 240
gag gac cgc ccg cag gtc ctc atc acc acc ccc tac ggt gcc ctg cgc 1068
Glu Asp Arg Pro Gln Val Leu Ile Thr Thr Pro Tyr Gly Ala Leu Arg
245 250 255
aac ggc ctc gac gcc ctc gtc gcc gcc aag ccg gag gcc ctg cag gtc 1116
Asn Gly Leu Asp Ala Leu Val Ala Ala Lys Pro Glu Ala Leu Gln Val
260 265 270
gac ctc gca ccg gtc acc ctc gcc aac gag gag ggc tac gtc gag cgc 1164
Asp Leu Ala Pro Val Thr Leu Ala Asn Glu Glu Gly Tyr Val Glu Arg
275 280 285
gtc gcc aag gca gtc gcc ggc acc ggc atc acc ctg gcc gcc ggc gtc 1212
Val Ala Lys Ala Val Ala Gly Thr Gly Ile Thr Leu Ala Ala Gly Val
290 295 300
atc gac ggc cgc aac atc tgg gcc gcc gac ctg cgc gag cgc ctc tcc 1260
Ile Asp Gly Arg Asn Ile Trp Ala Ala Asp Leu Arg Glu Arg Leu Ser
305 310 315 320
gtc ctg gag gag ctg aag aag ggc ggc gtc gag aag atc gcc gtc acc 1308
Val Leu Glu Glu Leu Lys Lys Gly Gly Val Glu Lys Ile Ala Val Thr
325 330 335
tcc tcc gtc tcc ctg cag cac gtc ccg cac acc gtc tcc gtg gag aag 1356
Ser Ser Val Ser Leu Gln His Val Pro His Thr Val Ser Val Glu Lys
340 345 350
aac ctc ccg gtc gac gtc gcc ggc tgg ctc tcc ttc gcc gac gag aag 1404
Asn Leu Pro Val Asp Val Ala Gly Trp Leu Ser Phe Ala Asp Glu Lys
355 360 365
atc ggc gag gca cag gcc ctg gcc acc gca ctc gag ggc ggc tcc gtc 1452
Ile Gly Glu Ala Gln Ala Leu Ala Thr Ala Leu Glu Gly Gly Ser Val
370 375 380
gcc gcc gca gac gcc ttc gcc cgc tcc gac cgc gcc gtg cgc acc cgt 1500
Ala Ala Ala Asp Ala Phe Ala Arg Ser Asp Arg Ala Val Arg Thr Arg
385 390 395 400
cgt gag tcc gcc cgc acc cac aac cag gcc gtc cag gac cgc gtc gcc 1548
Arg Glu Ser Ala Arg Thr His Asn Gln Ala Val Gln Asp Arg Val Ala
405 410 415
gct ctg ccg gac ggc cag gtc gca cgc gag ccg gag ttc gag ggc cgc 1596
Ala Leu Pro Asp Gly Gln Val Ala Arg Glu Pro Glu Phe Glu Gly Arg
420 425 430
gtc gag gca cag aag gtg ctc aac ctg ccg aag ctg ccg acc acc acg 1644
Val Glu Ala Gln Lys Val Leu Asn Leu Pro Lys Leu Pro Thr Thr Thr
435 440 445
atc ggc tcc ttc ccg cag acc acc gag atc cgc aag gca cgc gcc gac 1692
Ile Gly Ser Phe Pro Gln Thr Thr Glu Ile Arg Lys Ala Arg Ala Asp
450 455 460
cac cgc tcc ggc gcc ctc acc gac gag cag tac acc gac gcc ctc aag 1740
His Arg Ser Gly Ala Leu Thr Asp Glu Gln Tyr Thr Asp Ala Leu Lys
465 470 475 480
gac gag gtc aag tcc gtc atc gag ctg cag gag cgc ctc ggc atc gac 1788
Asp Glu Val Lys Ser Val Ile Glu Leu Gln Glu Arg Leu Gly Ile Asp
485 490 495
gtc ctc gtc cac ggc gag ccc gag cgc aac gac atg gtc cag tac ttc 1836
Val Leu Val His Gly Glu Pro Glu Arg Asn Asp Met Val Gln Tyr Phe
500 505 510
gcc gag ctt ctc gac ggc ttc gtg acc acc gag cac ggc tgg gtc cag 1884
Ala Glu Leu Leu Asp Gly Phe Val Thr Thr Glu His Gly Trp Val Gln
515 520 525
tcc tac ggc tcc cgc tgc acc cgc ccg tcc atc gtc gtc ggc gac gtc 1932
Ser Tyr Gly Ser Arg Cys Thr Arg Pro Ser Ile Val Val Gly Asp Val
530 535 540
tcc cgc ccg aag gcc atg acc gtc gag tgg gcc aag tac gcc cag tcc 1980
Ser Arg Pro Lys Ala Met Thr Val Glu Trp Ala Lys Tyr Ala Gln Ser
545 550 555 560
ctc tcc gac aag cac gtc aag ggc atg ctg acc ggc ccg gtc acc atc 2028
Leu Ser Asp Lys His Val Lys Gly Met Leu Thr Gly Pro Val Thr Ile
565 570 575
ctc gcg tgg tcc ttc acc cgt gac gac gtt ccg aag tcc gtc tcc gcc 2076
Leu Ala Trp Ser Phe Thr Arg Asp Asp Val Pro Lys Ser Val Ser Ala
580 585 590
gac cag atc ggc gtc gcc ctc gcc gac gag gtc gcc gac ctc gag gcc 2124
Asp Gln Ile Gly Val Ala Leu Ala Asp Glu Val Ala Asp Leu Glu Ala
595 600 605
gcc ggc atc aac gtc atc cag atc gac gag ccg gcc ctg cgc gag ctg 2172
Ala Gly Ile Asn Val Ile Gln Ile Asp Glu Pro Ala Leu Arg Glu Leu
610 615 620
ctg ccg ctg cgt gag gag gac cgc ccg gca tac ctg gac tgg gcc gtc 2220
Leu Pro Leu Arg Glu Glu Asp Arg Pro Ala Tyr Leu Asp Trp Ala Val
625 630 635 640
cgc tcc ttc cgc ctc gtc tcc ctg gac gcg aag ccg acc acc cag atc 2268
Arg Ser Phe Arg Leu Val Ser Leu Asp Ala Lys Pro Thr Thr Gln Ile
645 650 655
cac acc cac ctc tgc tac tcg gag ttc ggg cag atc atc gac gcc gtc 2316
His Thr His Leu Cys Tyr Ser Glu Phe Gly Gln Ile Ile Asp Ala Val
660 665 670
gcc gcc ctc gac gcc gac gtc acc tcc atc gag gcc gcc cgc tcc cgc 2364
Ala Ala Leu Asp Ala Asp Val Thr Ser Ile Glu Ala Ala Arg Ser Arg
675 680 685
atg gag ctg ctg ggt gac ctg gac gag tcc ttc cac tcc gag atc ggc 2412
Met Glu Leu Leu Gly Asp Leu Asp Glu Ser Phe His Ser Glu Ile Gly
690 695 700
ccg ggc atc tgg gac atc cac tcc ccg cgc gtc ccg gac gtc gcc gag 2460
Pro Gly Ile Trp Asp Ile His Ser Pro Arg Val Pro Asp Val Ala Glu
705 710 715 720
ctc acc gag ctg atc aag gcc gcc ctg gtc aac gtc ccg acc gag cgc 2508
Leu Thr Glu Leu Ile Lys Ala Ala Leu Val Asn Val Pro Thr Glu Arg
725 730 735
ctc tgg gtc aac ccg gac tgc ggt ctg aag acc cgc ggc tac gcc gag 2556
Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Tyr Ala Glu
740 745 750
acc gag gcg tcc ctg cgc aac atg gtc ctc gcc cgc gac ctc gtg gtc 2604
Thr Glu Ala Ser Leu Arg Asn Met Val Leu Ala Arg Asp Leu Val Val
755 760 765
gag tcc ctg taa cacctgcgta gcaactacgt tgcgggcccg gttccacttc 2656
Glu Ser Leu
770
ggtggggccg ggcccctcgt gattccgggg gcggctagcg ctcgtcgatg acctccgcga 2716
aggcggccac cttccggcgg agatcctcga tgagggcgtc cccgtccctc cgggacttct 2776
cctccgacgc cgggtcatcc cagcctgtct cccagtggtg gtgggaccgc cagtggtcgc 2836
accatgcgta gagccgctgc gtcagcgact cggacaggcc gtagtcccgt ggcgccatca 2896
gataggtgtc cgtgccgttc 2916
<210> 88
<211> 771
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 88
Met Ser Ser Thr Ala Phe Pro Thr Phe Thr Ile Glu Gly Tyr Pro Arg
1 5 10 15
Val Gly Ala Asn Arg Glu Leu Lys Lys Ala Leu Glu Ser Phe Trp Ala
20 25 30
Gly Arg Ile Asp Glu Glu Thr Phe Ile Ser Ser Ala His Ser Ile Arg
35 40 45
Leu Glu Asn Tyr Ala Arg Leu Arg Asp Leu Gly Leu Asp Gln Asp Tyr
50 55 60
Ala Ile Pro Ala Asp Val Ala Leu Tyr Asp Gln Val Leu Glu Thr Gly
65 70 75 80
Val Thr Val Gly Leu Ile Gly Gly Asp Ala Ala Glu Val Asp Leu Thr
85 90 95
Glu Tyr Phe Ala Leu Ala Arg Gly Thr Ala Glu Arg Ser Pro Leu Glu
100 105 110
Met Thr Lys Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Val
115 120 125
Ala Glu Asp Thr Val Ile Thr Pro Arg Pro Gln Arg Ile Leu Asp Ile
130 135 140
Val Ala Glu Ala Lys Glu Ala Gly His Thr Val Arg Pro Phe Leu Val
145 150 155 160
Gly Pro Val Thr Leu Leu Ala Lys Ser Lys Ala Ala Glu Asn Ala Gly
165 170 175
Glu Gly Phe Asn Pro Leu Ser Arg Leu Glu Asp Leu Thr Ala Ala Tyr
180 185 190
Ala Gln Val Leu Ala Ala Leu Lys Glu Ala Gly Val Glu Trp Val Gln
195 200 205
Leu Ala Glu Pro Ala Leu Val Ala Asp Leu Thr Ile Arg Ser Asp Ala
210 215 220
Asp Leu Ala Ala Asp Ala Lys Ala Thr Tyr Glu Ala Leu Leu Gly Ser
225 230 235 240
Glu Asp Arg Pro Gln Val Leu Ile Thr Thr Pro Tyr Gly Ala Leu Arg
245 250 255
Asn Gly Leu Asp Ala Leu Val Ala Ala Lys Pro Glu Ala Leu Gln Val
260 265 270
Asp Leu Ala Pro Val Thr Leu Ala Asn Glu Glu Gly Tyr Val Glu Arg
275 280 285
Val Ala Lys Ala Val Ala Gly Thr Gly Ile Thr Leu Ala Ala Gly Val
290 295 300
Ile Asp Gly Arg Asn Ile Trp Ala Ala Asp Leu Arg Glu Arg Leu Ser
305 310 315 320
Val Leu Glu Glu Leu Lys Lys Gly Gly Val Glu Lys Ile Ala Val Thr
325 330 335
Ser Ser Val Ser Leu Gln His Val Pro His Thr Val Ser Val Glu Lys
340 345 350
Asn Leu Pro Val Asp Val Ala Gly Trp Leu Ser Phe Ala Asp Glu Lys
355 360 365
Ile Gly Glu Ala Gln Ala Leu Ala Thr Ala Leu Glu Gly Gly Ser Val
370 375 380
Ala Ala Ala Asp Ala Phe Ala Arg Ser Asp Arg Ala Val Arg Thr Arg
385 390 395 400
Arg Glu Ser Ala Arg Thr His Asn Gln Ala Val Gln Asp Arg Val Ala
405 410 415
Ala Leu Pro Asp Gly Gln Val Ala Arg Glu Pro Glu Phe Glu Gly Arg
420 425 430
Val Glu Ala Gln Lys Val Leu Asn Leu Pro Lys Leu Pro Thr Thr Thr
435 440 445
Ile Gly Ser Phe Pro Gln Thr Thr Glu Ile Arg Lys Ala Arg Ala Asp
450 455 460
His Arg Ser Gly Ala Leu Thr Asp Glu Gln Tyr Thr Asp Ala Leu Lys
465 470 475 480
Asp Glu Val Lys Ser Val Ile Glu Leu Gln Glu Arg Leu Gly Ile Asp
485 490 495
Val Leu Val His Gly Glu Pro Glu Arg Asn Asp Met Val Gln Tyr Phe
500 505 510
Ala Glu Leu Leu Asp Gly Phe Val Thr Thr Glu His Gly Trp Val Gln
515 520 525
Ser Tyr Gly Ser Arg Cys Thr Arg Pro Ser Ile Val Val Gly Asp Val
530 535 540
Ser Arg Pro Lys Ala Met Thr Val Glu Trp Ala Lys Tyr Ala Gln Ser
545 550 555 560
Leu Ser Asp Lys His Val Lys Gly Met Leu Thr Gly Pro Val Thr Ile
565 570 575
Leu Ala Trp Ser Phe Thr Arg Asp Asp Val Pro Lys Ser Val Ser Ala
580 585 590
Asp Gln Ile Gly Val Ala Leu Ala Asp Glu Val Ala Asp Leu Glu Ala
595 600 605
Ala Gly Ile Asn Val Ile Gln Ile Asp Glu Pro Ala Leu Arg Glu Leu
610 615 620
Leu Pro Leu Arg Glu Glu Asp Arg Pro Ala Tyr Leu Asp Trp Ala Val
625 630 635 640
Arg Ser Phe Arg Leu Val Ser Leu Asp Ala Lys Pro Thr Thr Gln Ile
645 650 655
His Thr His Leu Cys Tyr Ser Glu Phe Gly Gln Ile Ile Asp Ala Val
660 665 670
Ala Ala Leu Asp Ala Asp Val Thr Ser Ile Glu Ala Ala Arg Ser Arg
675 680 685
Met Glu Leu Leu Gly Asp Leu Asp Glu Ser Phe His Ser Glu Ile Gly
690 695 700
Pro Gly Ile Trp Asp Ile His Ser Pro Arg Val Pro Asp Val Ala Glu
705 710 715 720
Leu Thr Glu Leu Ile Lys Ala Ala Leu Val Asn Val Pro Thr Glu Arg
725 730 735
Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Tyr Ala Glu
740 745 750
Thr Glu Ala Ser Leu Arg Asn Met Val Leu Ala Arg Asp Leu Val Val
755 760 765
Glu Ser Leu
770
<210> 89
<211> 1200
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(900)
<223> ген ptH1
<400> 89
gcgaacttgg cgtcgaagtc gttcgacagg tcgacctccg gcagggggcg gtcgccggcg 60
acacggcgga cgtcggcaag cgactcgaag ggcgcctgcc gcgtgaacag attgacggcg 120
tagggccccg tgacctgcgc cagctgctcc tccaggagcg cggcgggcgc gccgcccggc 180
gcgaggaaac cgagggaacc ggcggctgcg gcggcgtcga caagcgcggg cgacgagggg 240
ccgccggcca tgggcgcgac gatgatctgc gggatgtcca tgtgtgagag actatcgggc 300
gtg ttc aag atc ttc gac ctc ttc cgc cgc aag cag acg acc ccg gcg 348
Val Phe Lys Ile Phe Asp Leu Phe Arg Arg Lys Gln Thr Thr Pro Ala
1 5 10 15
ctc agc gcc gac tgg atc atc ctc ggc ctg ggc aac ccg ggg ccg aaa 396
Leu Ser Ala Asp Trp Ile Ile Leu Gly Leu Gly Asn Pro Gly Pro Lys
20 25 30
tac gcc gcc acg cgc cac aac gtg ggc tac atg gcc gtc gac gag ctt 444
Tyr Ala Ala Thr Arg His Asn Val Gly Tyr Met Ala Val Asp Glu Leu
35 40 45
ctc gac gcc acc ccg ctc cag ccc ctc ccc ggt acg aag gca ctg cac 492
Leu Asp Ala Thr Pro Leu Gln Pro Leu Pro Gly Thr Lys Ala Leu His
50 55 60
gcg aag acg atg atc gcg ggg cag gac gtg ctc gtg ctg cgt tcg acg 540
Ala Lys Thr Met Ile Ala Gly Gln Asp Val Leu Val Leu Arg Ser Thr
65 70 75 80
acc tac atg aac ctc tcc ggc gag gct gtc gcc cca ctc gcg gag cag 588
Thr Tyr Met Asn Leu Ser Gly Glu Ala Val Ala Pro Leu Ala Glu Gln
85 90 95
ctc ggg gtg ccg gcg gag cgg atc atc gcc atc cac gac gag ctc gac 636
Leu Gly Val Pro Ala Glu Arg Ile Ile Ala Ile His Asp Glu Leu Asp
100 105 110
ctg ccg gtg ggc acc gtg cgg gtg aag aag ggc ggc aac gag aac ggc 684
Leu Pro Val Gly Thr Val Arg Val Lys Lys Gly Gly Asn Glu Asn Gly
115 120 125
cac aac ggc ctg aag tcc atg acc gag cac ctg ggc acc cgc gac tac 732
His Asn Gly Leu Lys Ser Met Thr Glu His Leu Gly Thr Arg Asp Tyr
130 135 140
ctg cgg gtg cgc atc ggc atc gcc cgc ccg ccg cag ggc gtg tcg gtg 780
Leu Arg Val Arg Ile Gly Ile Ala Arg Pro Pro Gln Gly Val Ser Val
145 150 155 160
acc gac cat gtc ctc ggc ccc atc gac ggc ggc gag aac atc cgc ctg 828
Thr Asp His Val Leu Gly Pro Ile Asp Gly Gly Glu Asn Ile Arg Leu
165 170 175
gcc gcc gag gcg gcg cgg ctc atc gtc gag cag ggg ctg ggc agg gcg 876
Ala Ala Glu Ala Ala Arg Leu Ile Val Glu Gln Gly Leu Gly Arg Ala
180 185 190
cag aac gtc atc cac gcg cgg tga tcgcccccgg gcgctgctgc accaaagggg 930
Gln Asn Val Ile His Ala Arg
195
gtattcgagt cggggcattg atcgggtgac gcccacgcgc tacccgatca atgcctcagt 990
tcgaataccc cctcggcccc ggcggccccg caggatctac ttcgccatct gcgcgacgag 1050
cttctccagg tcgccgagca tcgtctccag ctcgccgagg cgacgctcct gcggggagaa 1110
cacgccctcc tccgcgtcgt tgaaggtcga caggtacacc tggttgcgca cgtcgtactg 1170
ctggaagttg ccgacgatct gccgccagct 1200
<210> 90
<211> 199
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 90
Val Phe Lys Ile Phe Asp Leu Phe Arg Arg Lys Gln Thr Thr Pro Ala
1 5 10 15
Leu Ser Ala Asp Trp Ile Ile Leu Gly Leu Gly Asn Pro Gly Pro Lys
20 25 30
Tyr Ala Ala Thr Arg His Asn Val Gly Tyr Met Ala Val Asp Glu Leu
35 40 45
Leu Asp Ala Thr Pro Leu Gln Pro Leu Pro Gly Thr Lys Ala Leu His
50 55 60
Ala Lys Thr Met Ile Ala Gly Gln Asp Val Leu Val Leu Arg Ser Thr
65 70 75 80
Thr Tyr Met Asn Leu Ser Gly Glu Ala Val Ala Pro Leu Ala Glu Gln
85 90 95
Leu Gly Val Pro Ala Glu Arg Ile Ile Ala Ile His Asp Glu Leu Asp
100 105 110
Leu Pro Val Gly Thr Val Arg Val Lys Lys Gly Gly Asn Glu Asn Gly
115 120 125
His Asn Gly Leu Lys Ser Met Thr Glu His Leu Gly Thr Arg Asp Tyr
130 135 140
Leu Arg Val Arg Ile Gly Ile Ala Arg Pro Pro Gln Gly Val Ser Val
145 150 155 160
Thr Asp His Val Leu Gly Pro Ile Asp Gly Gly Glu Asn Ile Arg Leu
165 170 175
Ala Ala Glu Ala Ala Arg Leu Ile Val Glu Gln Gly Leu Gly Arg Ala
180 185 190
Gln Asn Val Ile His Ala Arg
195
<210> 91
<211> 1146
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(846)
<223> ген ptH2
<400> 91
aggagatcgt cgtgtccgtc gagggcatcg aggccggcaa cgtcatcaac gccggtgaca 60
tcaccctgcc ggaggacgtc accctggtcg acgacgccga gctgctcgtc ttcaacatca 120
ccttcgagga ggtcgccgac gttccggagg agggcgagga ggccaccgaa gacgctgtcg 180
gcgaggccgg caaggacgag gctgccgagg gcgaggagta gtcactcccc ttccgccacg 240
tgtgacgccc ctgaccggcc ggtcaggggc gtctcccctt ttctgtgcca gactgatcgg 300
gtg act gat tct cca ctt ctc atc gtc ggc ctg ggt aac ccg ggc gcg 348
Val Thr Asp Ser Pro Leu Leu Ile Val Gly Leu Gly Asn Pro Gly Ala
1 5 10 15
aag tac gcc gac acc cgc cac aac atc ggc gtc atg ctt ctc gac gag 396
Lys Tyr Ala Asp Thr Arg His Asn Ile Gly Val Met Leu Leu Asp Glu
20 25 30
ctc gcc tcc cgc atc acc ccc gtc ccc gcc agc ttc gcc gtg cac aag 444
Leu Ala Ser Arg Ile Thr Pro Val Pro Ala Ser Phe Ala Val His Lys
35 40 45
cgc acg aac acc gag gtc gcc gag gtc cgt cat cgc ggc cgt cgc ctc 492
Arg Thr Asn Thr Glu Val Ala Glu Val Arg His Arg Gly Arg Arg Leu
50 55 60
gtg ctg gcc cgg acc cgc ggc tac atg aac gtc tcc ggt gga ccg gtg 540
Val Leu Ala Arg Thr Arg Gly Tyr Met Asn Val Ser Gly Gly Pro Val
65 70 75 80
aag gca ctg gcg acc ttc ttc agg atc gcc ccc ggc gac atc atc gtc 588
Lys Ala Leu Ala Thr Phe Phe Arg Ile Ala Pro Gly Asp Ile Ile Val
85 90 95
gcc cac gac gaa ctc gag ctg gac ttc ggc gag cac cgc ctc cgc acc 636
Ala His Asp Glu Leu Glu Leu Asp Phe Gly Glu His Arg Leu Arg Thr
100 105 110
ggc ggc ggc gac cac ggc cac aac ggc ctg aag tcc gtg acc aag tcg 684
Gly Gly Gly Asp His Gly His Asn Gly Leu Lys Ser Val Thr Lys Ser
115 120 125
ctg gga acg aag gag tac cag cgc ctg gcg ctc ggc atc ggc cgt ccg 732
Leu Gly Thr Lys Glu Tyr Gln Arg Leu Ala Leu Gly Ile Gly Arg Pro
130 135 140
ccc ggg cgt atg gac ccc gcc gcc ttc gtg ctc aag ccc ttc acc cgc 780
Pro Gly Arg Met Asp Pro Ala Ala Phe Val Leu Lys Pro Phe Thr Arg
145 150 155 160
cag gag gcc ggg gag ctg ccc ttc ctg ctc tcc gac gcc gcc gac gaa 828
Gln Glu Ala Gly Glu Leu Pro Phe Leu Leu Ser Asp Ala Ala Asp Glu
165 170 175
ctc gaa cgc gcc ctc tga cctcccaggg tggaacctgg tcccggaagg 876
Leu Glu Arg Ala Leu
180
ttccggacgc tgcccgggtg tctagatggt gagctcctcc ggggccgcct ccccctcgcg 936
ccgggcgatg atcttgtgga tctggtactc ccactcctgc agctcctccg gcagctcccc 996
cttcgagggg tcacggagat actcgcgacg cccggcgtcg tagccccaca gccagtcgac 1056
gtagcggtac caggacgtgc ggccggcctg gcggaaggtc tcgttgcgga tcaggcggga 1116
gacgccgtcg acgtgccaca gctcccccca 1146
<210> 92
<211> 181
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 92
Val Thr Asp Ser Pro Leu Leu Ile Val Gly Leu Gly Asn Pro Gly Ala
1 5 10 15
Lys Tyr Ala Asp Thr Arg His Asn Ile Gly Val Met Leu Leu Asp Glu
20 25 30
Leu Ala Ser Arg Ile Thr Pro Val Pro Ala Ser Phe Ala Val His Lys
35 40 45
Arg Thr Asn Thr Glu Val Ala Glu Val Arg His Arg Gly Arg Arg Leu
50 55 60
Val Leu Ala Arg Thr Arg Gly Tyr Met Asn Val Ser Gly Gly Pro Val
65 70 75 80
Lys Ala Leu Ala Thr Phe Phe Arg Ile Ala Pro Gly Asp Ile Ile Val
85 90 95
Ala His Asp Glu Leu Glu Leu Asp Phe Gly Glu His Arg Leu Arg Thr
100 105 110
Gly Gly Gly Asp His Gly His Asn Gly Leu Lys Ser Val Thr Lys Ser
115 120 125
Leu Gly Thr Lys Glu Tyr Gln Arg Leu Ala Leu Gly Ile Gly Arg Pro
130 135 140
Pro Gly Arg Met Asp Pro Ala Ala Phe Val Leu Lys Pro Phe Thr Arg
145 150 155 160
Gln Glu Ala Gly Glu Leu Pro Phe Leu Leu Ser Asp Ala Ala Asp Glu
165 170 175
Leu Glu Arg Ala Leu
180
<210> 93
<211> 1389
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1089)
<223> ген lplA
<400> 93
gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct acgcggagct 60
cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg agtccacgaa 120
gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca acgacgcggt 180
ccacgacgac tacgccgtga tcaacgacga ccccttcggc gcgggctggc tcttcgccgt 240
cgaggtcgac gaggtcggac cgctcatgac cgccgccgag tacgcggagg ccaacggcgt 300
gtg acc gcc cgg gac cgg tac cct ggt ccc atc atg act gca ccc cgt 348
Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg
1 5 10 15
gag ccc ttc ttc cct gcg gac cgt tcc atc cgt gcc tcc ggc gag ccc 396
Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro
20 25 30
ccg gag atc cgt tat ctc ggc gtc gtc gac tac cag gag gcc tgg aat 444
Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn
35 40 45
ctg cag gcc gag ctg gcg tcg caa cgc gcg gcc ggg gag atc ggt gac 492
Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp
50 55 60
cag ctc ctc atc ctc gag cac ccg agc gtg tac acc gcc ggc aag cgc 540
Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg
65 70 75 80
acg cag ccg gag gac cgc ccc acc aac ggc ctg ccg gtc atc gac gtc 588
Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val
85 90 95
gac cgg ggt ggc cgc atc acc tgg cac ggc gag gga cag ctg gtg gtc 636
Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val
100 105 110
tac ccg atc atc aaa ctg gcc gac ccg gtg gac gtc gtg gac tat gtc 684
Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val
115 120 125
cgc cgc ctc gag gag gcg gtg atc gcg acg gtg cgg gag gtg ggc gtc 732
Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val
130 135 140
ggc aat gcg ggt cgc atc gac ggc cgc tcc gga gtc tgg gtc ccc gcc 780
Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala
145 150 155 160
gac ggg acc cga cgc gac cgg aag gtg gcg gcg ctc ggc atc cgt gtc 828
Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val
165 170 175
acc cgc ggg gtg acc atg cac ggg ctg agc ctg aac tgc tcg aac acg 876
Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr
180 185 190
ctc gag cac tac ggg cac atc gtg gcg tgc ggc atc gac gac gcc gac 924
Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp
195 200 205
gtc acc acc ctc agc atg gaa ctg ggg cgc gac gtc agc gtc ggg gag 972
Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu
210 215 220
gtc acg gca ccg ctt gtc gac gcc ctc ctg gcc gcc ctc gcc ggt gaa 1020
Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu
225 230 235 240
ctc gcc gtc gcg gac cac acc ttc gga tcg gcg ccc gat ccg acg aag 1068
Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys
245 250 255
att ccc gcg aaa acg cgc tga cctctaagtt tatccctatc agtagaaaga 1119
Ile Pro Ala Lys Thr Arg
260
gtatggtggc gttgtgacta tcgcacctga aggacgcaag ctgctccggg tggagaagcg 1179
caatgcgcag acccccatcg agacgaagcc ccgttggatc cgcaacgcgg tgaagaccgg 1239
ccccgagtac gaggacatga agaagaaggt ggcgggcgcc tccctgcaca ccgtctgtca 1299
ggaggcgggc tgccccaaca tccacgagtg ctgggagtcc cgcgaggcga cgtttctcat 1359
cggcggcgcc aactgctccc gccgctgcga 1389
<210> 94
<211> 262
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 94
Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg
1 5 10 15
Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro
20 25 30
Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn
35 40 45
Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp
50 55 60
Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg
65 70 75 80
Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val
85 90 95
Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val
100 105 110
Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val
115 120 125
Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val
130 135 140
Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala
145 150 155 160
Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val
165 170 175
Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr
180 185 190
Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp
195 200 205
Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu
210 215 220
Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu
225 230 235 240
Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys
245 250 255
Ile Pro Ala Lys Thr Arg
260
<210> 95
<211> 2010
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1710)
<223> ген gcvL
<400> 95
caactgacat cacagagaga aggcacacat gtccatcttc gagcagatca tcgtcgccat 60
caacaacttc gtcggcccgg gcatccacgc cctgtcctcc gcttcctcca acgccgcact 120
ggccatcggc ctcttctagg tcgaccccgg aagcatctgc ttatcgacgc cccggccccc 180
ggtcggggcg ttcgtcgttg cggcaccaca gtcccgccac cgtcccaccg cccccacggg 240
ggcgggatcc ttttgtgggg ggcggtgaca cgggggaaac ggtcacctat ccttggtggc 300
gtg act gaa cat tat gac gtt gtt gta ctc ggt gcg ggc ccc ggt ggc 348
Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly
1 5 10 15
tac gtc gcc gcc atc cgc gca gcc cag ctc ggc aag aag gtt gct gtc 396
Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val
20 25 30
gtc gag aag cag tac tgg ggc gga gtc tgc ctc aac gtg ggc tgc atc 444
Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile
35 40 45
ccc tcg aag gcg ctg atc aag aac gcc gag atc gcc cac atc ttc acg 492
Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr
50 55 60
cac gag aag aag acg ttc ggc atc aac ggc gac gtc tcc ttc aac tac 540
His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr
65 70 75 80
gag gac gcc cac aag cgc tcc cgt cag gtg tcg gag aag atc gtc ggc 588
Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly
85 90 95
ggc gtc cac tac ctg atg aag aag aac aag atc acc gag atc gac ggc 636
Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly
100 105 110
ttc gga acc ttc agg gac gcc aag acc atc gag gtc agc gag ggc aag 684
Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys
115 120 125
gac gcc ggc aag gtc gtc acc ttc gac gac tgc atc atc gcc acc ggc 732
Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly
130 135 140
tcc gtg gtc aac agc ctc cgt ggc gtc gag ttc tcc gag aac gtc gtc 780
Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val
145 150 155 160
tcc tac gag gag cag atc ctc aac ccg gtc gcc ccg gag aag atg gtc 828
Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val
165 170 175
atc gtc ggt gcc ggc gcc atc ggc atg gag ttc gcc tac gtg ctc tcc 876
Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser
180 185 190
aac tac ggc gtc gac gtc acc gtc atc gag tac atg gac cgt gtc ctg 924
Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu
195 200 205
ccg aac gag gac gag gac gtc tcc aag gtc atc gcc aag gcc tac aag 972
Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys
210 215 220
aag atg ggc gtc acg ctc ctc ccg ggc cac gcc acc acc gcc gtg cgc 1020
Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg
225 230 235 240
gac aac ggc gac tcc gtc gag gtc gac tac cag aag aag ggc tcg gac 1068
Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp
245 250 255
aag acc gag acc atc gtc gcc gac cgc gtc ctc gtc tcc gtc ggc ttc 1116
Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe
260 265 270
cgc ccg cgt gtc gag ggc ttc ggc ctg gag aac acc ggc gtc gag ctc 1164
Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu
275 280 285
acc gag cgc ggt gcc atc gcc atc gac gac ttc atg cgc acc aac gtc 1212
Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val
290 295 300
gag cac atc tac gcc atc ggt gac gtc acc gcc aag ctg cag ctg gcc 1260
Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala
305 310 315 320
cac gtc gcc gag gcg cag ggc atc gtc gcc gcc gag acc atc gcc ggc 1308
His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly
325 330 335
gcc gag acc cac gag ctg ggc gac tac atg atg atg ccg cgc gcc acc 1356
Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr
340 345 350
ttc tgt aac ccg cag gtc gcg tcc ttc ggc tac acc gag gcg cag gcc 1404
Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala
355 360 365
aag gag aag tgg ccg gac cgc gag atc aag gtc gcg tcc ttc ccg ttc 1452
Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe
370 375 380
tcc gcg aac ggc aag gcc gtc ggc ctg gcc gag acc gag ggc ttc gcc 1500
Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala
385 390 395 400
aag atc gtc gcc gac gcc gag tac ggc gag ctg ctg ggc ggc cac ctg 1548
Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu
405 410 415
gtc ggc gcc aac gcg tcc gag ctg ctc aac gag ctg gtc ctc gcg cag 1596
Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln
420 425 430
aac tac gat ctc acc acc gag gag atc agc cgc gcg gtc cac atc cac 1644
Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His
435 440 445
ccg acc ctg tcg gag gcc gtc aag gag gcc gca cac ggc atc gac ggc 1692
Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly
450 455 460
cac atg atc aac ttc tag tcccccggaa aaaggggcct cccggctggg 1740
His Met Ile Asn Phe
465
aggccccttt tctcatgctt tccgacgccc ccggggcgtc gccaagcgcc tagttgtagg 1800
gcgcgaccga cgaccggtgg gcgtcgatgg tgatctcctc gtggatcggc gggaacgcgc 1860
gctgcgcgca gttctcccgc gggcacacgc ggcagccggc gccgatcggg gtggcgctgg 1920
acaggtcggc gaggttgagt ccctgcgcgt agaccgtgcg gtcggcgtgg cgggcctcgc 1980
agcccaggcc gatggcgaag agcttgccgg 2010
<210> 96
<211> 469
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 96
Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly
1 5 10 15
Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val
20 25 30
Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile
35 40 45
Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr
50 55 60
His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr
65 70 75 80
Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly
85 90 95
Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly
100 105 110
Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys
115 120 125
Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly
130 135 140
Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val
145 150 155 160
Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val
165 170 175
Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser
180 185 190
Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu
195 200 205
Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys
210 215 220
Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg
225 230 235 240
Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp
245 250 255
Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe
260 265 270
Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu
275 280 285
Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val
290 295 300
Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala
305 310 315 320
His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly
325 330 335
Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr
340 345 350
Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala
355 360 365
Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe
370 375 380
Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala
385 390 395 400
Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu
405 410 415
Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln
420 425 430
Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His
435 440 445
Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly
450 455 460
His Met Ile Asn Phe
465
<210> 97
<211> 2442
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2142)
<223> ген ilvB
<400> 97
cttgtggtag cagcgcggcg cctgccgtaa cgaccctacc cagtcgtctc gacaagcgcc 60
ctcgacagca cccaccatca gtgctgtacg ggggctttgt ctttagatgg tcgcaggtca 120
cttgtgccga cgacagccac caggccgacc ccttccgcac caagggaaga gtcaccaggt 180
agcgtttcaa ttgcattgtc cttgtgaaat taggagcttg ataacgtggc agcttcgcaa 240
acgcccaccc cggccacggt cgccaagaag tccccgggag ccgagaacgc acccgagcgg 300
atg acc ggc gcc cag gcg atc gtc cgg tcc ctc gag gag ctc ggc acc 348
Met Thr Gly Ala Gln Ala Ile Val Arg Ser Leu Glu Glu Leu Gly Thr
1 5 10 15
gac atc gtc ttc ggc ctg ccc ggc ggt gcc atc ctc ccc ctg tac gac 396
Asp Ile Val Phe Gly Leu Pro Gly Gly Ala Ile Leu Pro Leu Tyr Asp
20 25 30
ccc ctc ttc tcc tcg aag aag ctg cgt cac gtg ctg gtg cgc cac gag 444
Pro Leu Phe Ser Ser Lys Lys Leu Arg His Val Leu Val Arg His Glu
35 40 45
cag ggc tcg ggc cac gct gcc acc ggt tac gca cag gtc acc ggc aag 492
Gln Gly Ser Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Lys
50 55 60
gtc ggc gtc tgc atc gcc acc tcc ggc ccg ggt gcc acc aac ctg gtc 540
Val Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val
65 70 75 80
acc ccg ctg gcc gac gcc aac ctg gac tcc gtc ccg atg gtc gcc att 588
Thr Pro Leu Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile
85 90 95
acc ggc cag gtc ggc cgt tcc ctg ctg ggc acc gac gcc ttc cag gag 636
Thr Gly Gln Val Gly Arg Ser Leu Leu Gly Thr Asp Ala Phe Gln Glu
100 105 110
gcc gac atc cgg ggt gtg acc atg ccg atc acg aag cac aac ttc atg 684
Ala Asp Ile Arg Gly Val Thr Met Pro Ile Thr Lys His Asn Phe Met
115 120 125
gtc acc gac ccc aac gac atc gcc cgc acc ctg atg gag gcc ttc cac 732
Val Thr Asp Pro Asn Asp Ile Ala Arg Thr Leu Met Glu Ala Phe His
130 135 140
ctc gct ctg acc ggt cgc ccg ggc ccc gtc ctg gtc gac atc ccg aag 780
Leu Ala Leu Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys
145 150 155 160
gac atc cag aac gcg gag ttc gac ttc gtc tgg ccg ccg aag atg gat 828
Asp Ile Gln Asn Ala Glu Phe Asp Phe Val Trp Pro Pro Lys Met Asp
165 170 175
ctg ccg ggc tac aag ccg gtc acc acg ccg cac tcc cgc cag atc acc 876
Leu Pro Gly Tyr Lys Pro Val Thr Thr Pro His Ser Arg Gln Ile Thr
180 185 190
cag gcc gtc cag atg atc gcc gag gcc gag cgt ccc tgc ctc tac gtc 924
Gln Ala Val Gln Met Ile Ala Glu Ala Glu Arg Pro Cys Leu Tyr Val
195 200 205
ggc ggc ggc gtc atc aag gcc gac gcc aac aag gag ctg ctg gag ttc 972
Gly Gly Gly Val Ile Lys Ala Asp Ala Asn Lys Glu Leu Leu Glu Phe
210 215 220
gcc gag tac acc ggc gtc ccg gtc gtc acc acc ctc atg gcc ctg ggc 1020
Ala Glu Tyr Thr Gly Val Pro Val Val Thr Thr Leu Met Ala Leu Gly
225 230 235 240
gcg ttc ccg gag tcc cac ccg ctg cac atg ggc atg ccg ggc atg cac 1068
Ala Phe Pro Glu Ser His Pro Leu His Met Gly Met Pro Gly Met His
245 250 255
ggc gcc gtc ccg gcc gtc ggt gcc atg cag ggc gct gac ctg ctc atc 1116
Gly Ala Val Pro Ala Val Gly Ala Met Gln Gly Ala Asp Leu Leu Ile
260 265 270
acc atc ggc gcg cgt ttc gac gac cgc gtc acc ggc aac ctc gac gag 1164
Thr Ile Gly Ala Arg Phe Asp Asp Arg Val Thr Gly Asn Leu Asp Glu
275 280 285
ttc gcc ccg gac gcc cag gtc atc cac gcc gac atc gac ccg gcc gag 1212
Phe Ala Pro Asp Ala Gln Val Ile His Ala Asp Ile Asp Pro Ala Glu
290 295 300
atc ggc aag atc cgc gag gcc gcc gtc ccg atc gtc ggc gac gcc cgc 1260
Ile Gly Lys Ile Arg Glu Ala Ala Val Pro Ile Val Gly Asp Ala Arg
305 310 315 320
gag gtc ctc tcc gca ctc ctg cgc acc tac aag tcc tcc aag aac ctc 1308
Glu Val Leu Ser Ala Leu Leu Arg Thr Tyr Lys Ser Ser Lys Asn Leu
325 330 335
acc ccg ccg aag atc acc gag tgg gtc gag tac ctg acg gac ctc aag 1356
Thr Pro Pro Lys Ile Thr Glu Trp Val Glu Tyr Leu Thr Asp Leu Lys
340 345 350
gag cgc ttc ccg cgc ggc tac gac gag cag tcg gac ggc atg atg agc 1404
Glu Arg Phe Pro Arg Gly Tyr Asp Glu Gln Ser Asp Gly Met Met Ser
355 360 365
ccg cag ttc gtc atc gag acc ctg tcc aag gag gtc ggc ccg gac gcg 1452
Pro Gln Phe Val Ile Glu Thr Leu Ser Lys Glu Val Gly Pro Asp Ala
370 375 380
atc tac gtc gcc ggc gtc ggc cag cac cag atg tgg gcc gcg cag ttc 1500
Ile Tyr Val Ala Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe
385 390 395 400
atc gac ttc gag aag ccg cgc acc tgg ctc aac tcc ggt ggt ctg ggc 1548
Ile Asp Phe Glu Lys Pro Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly
405 410 415
acg atg ggc tac tcc atc ccc gcc gcc atg ggc gcc aag gcc ggt ctg 1596
Thr Met Gly Tyr Ser Ile Pro Ala Ala Met Gly Ala Lys Ala Gly Leu
420 425 430
ccg gac gcc gag gtc tgg gcc atc gac ggt gac ggc tgc ttc cag atg 1644
Pro Asp Ala Glu Val Trp Ala Ile Asp Gly Asp Gly Cys Phe Gln Met
435 440 445
acc aac cag gag ctc acc acc gcc gcg gtc gag ggc ttc ccg atc aag 1692
Thr Asn Gln Glu Leu Thr Thr Ala Ala Val Glu Gly Phe Pro Ile Lys
450 455 460
gtc gcc ctg atc aac aac ggc aac ctg ggc atg gtc cgc cag tgg cag 1740
Val Ala Leu Ile Asn Asn Gly Asn Leu Gly Met Val Arg Gln Trp Gln
465 470 475 480
acc ctg ttc tac ggt ggc cgc tac tcg aac acg aag ctg cgt gag cag 1788
Thr Leu Phe Tyr Gly Gly Arg Tyr Ser Asn Thr Lys Leu Arg Glu Gln
485 490 495
gac gag tac gtg ccg gac ttc gtc acg ctg tcc gag gcg ctc ggc tgc 1836
Asp Glu Tyr Val Pro Asp Phe Val Thr Leu Ser Glu Ala Leu Gly Cys
500 505 510
gtc gcc ttc cgc gtc acc cgc gag gag gac gtc ctg ccg acg atc cag 1884
Val Ala Phe Arg Val Thr Arg Glu Glu Asp Val Leu Pro Thr Ile Gln
515 520 525
aag gca cgc gag atc aac gac cgc ccg gtc gtc atc gac ttc atc gtc 1932
Lys Ala Arg Glu Ile Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val
530 535 540
ggt gag gac gcc cag gtc tgg ccg atg gtc gcc gcc ggc gcc tcc aac 1980
Gly Glu Asp Ala Gln Val Trp Pro Met Val Ala Ala Gly Ala Ser Asn
545 550 555 560
tcg gac atc gag tac gca cgt ggt ctg cgc ccc ttc ttc gag ggt gac 2028
Ser Asp Ile Glu Tyr Ala Arg Gly Leu Arg Pro Phe Phe Glu Gly Asp
565 570 575
gag tcc gcg ggt gag tcc ccg gcc gac atc cac ggt gtc atg gag aac 2076
Glu Ser Ala Gly Glu Ser Pro Ala Asp Ile His Gly Val Met Glu Asn
580 585 590
gcc cag gag gag acc gtc gag gaa gtc ctc gag gag cgc gcg aac gac 2124
Ala Gln Glu Glu Thr Val Glu Glu Val Leu Glu Glu Arg Ala Asn Asp
595 600 605
agc gag aag gag aac tag gacaatggcg aacaccgaca tcatccgcaa 2172
Ser Glu Lys Glu Asn
610
caccctcagc gtcctggtcc aggacgtcga cggcatcatc tcccgcgtcg caggcatgtt 2232
cacccgccgt ggctacaacc tggtgtccat cgtctcggcg cacaccgaca ccccgggcat 2292
cagccgcatc accatcgtgg tcgacgccaa cgagctggtc atcgagcagg tcaccaagca 2352
gctcaacaag atcatcccgg tgctcaaggt cgtccgcctc gaggaggaca ccaccatcgc 2412
ccgcgccatc atgctggtga aggtcaacgc 2442
<210> 98
<211> 613
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 98
Met Thr Gly Ala Gln Ala Ile Val Arg Ser Leu Glu Glu Leu Gly Thr
1 5 10 15
Asp Ile Val Phe Gly Leu Pro Gly Gly Ala Ile Leu Pro Leu Tyr Asp
20 25 30
Pro Leu Phe Ser Ser Lys Lys Leu Arg His Val Leu Val Arg His Glu
35 40 45
Gln Gly Ser Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Lys
50 55 60
Val Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val
65 70 75 80
Thr Pro Leu Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile
85 90 95
Thr Gly Gln Val Gly Arg Ser Leu Leu Gly Thr Asp Ala Phe Gln Glu
100 105 110
Ala Asp Ile Arg Gly Val Thr Met Pro Ile Thr Lys His Asn Phe Met
115 120 125
Val Thr Asp Pro Asn Asp Ile Ala Arg Thr Leu Met Glu Ala Phe His
130 135 140
Leu Ala Leu Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys
145 150 155 160
Asp Ile Gln Asn Ala Glu Phe Asp Phe Val Trp Pro Pro Lys Met Asp
165 170 175
Leu Pro Gly Tyr Lys Pro Val Thr Thr Pro His Ser Arg Gln Ile Thr
180 185 190
Gln Ala Val Gln Met Ile Ala Glu Ala Glu Arg Pro Cys Leu Tyr Val
195 200 205
Gly Gly Gly Val Ile Lys Ala Asp Ala Asn Lys Glu Leu Leu Glu Phe
210 215 220
Ala Glu Tyr Thr Gly Val Pro Val Val Thr Thr Leu Met Ala Leu Gly
225 230 235 240
Ala Phe Pro Glu Ser His Pro Leu His Met Gly Met Pro Gly Met His
245 250 255
Gly Ala Val Pro Ala Val Gly Ala Met Gln Gly Ala Asp Leu Leu Ile
260 265 270
Thr Ile Gly Ala Arg Phe Asp Asp Arg Val Thr Gly Asn Leu Asp Glu
275 280 285
Phe Ala Pro Asp Ala Gln Val Ile His Ala Asp Ile Asp Pro Ala Glu
290 295 300
Ile Gly Lys Ile Arg Glu Ala Ala Val Pro Ile Val Gly Asp Ala Arg
305 310 315 320
Glu Val Leu Ser Ala Leu Leu Arg Thr Tyr Lys Ser Ser Lys Asn Leu
325 330 335
Thr Pro Pro Lys Ile Thr Glu Trp Val Glu Tyr Leu Thr Asp Leu Lys
340 345 350
Glu Arg Phe Pro Arg Gly Tyr Asp Glu Gln Ser Asp Gly Met Met Ser
355 360 365
Pro Gln Phe Val Ile Glu Thr Leu Ser Lys Glu Val Gly Pro Asp Ala
370 375 380
Ile Tyr Val Ala Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe
385 390 395 400
Ile Asp Phe Glu Lys Pro Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly
405 410 415
Thr Met Gly Tyr Ser Ile Pro Ala Ala Met Gly Ala Lys Ala Gly Leu
420 425 430
Pro Asp Ala Glu Val Trp Ala Ile Asp Gly Asp Gly Cys Phe Gln Met
435 440 445
Thr Asn Gln Glu Leu Thr Thr Ala Ala Val Glu Gly Phe Pro Ile Lys
450 455 460
Val Ala Leu Ile Asn Asn Gly Asn Leu Gly Met Val Arg Gln Trp Gln
465 470 475 480
Thr Leu Phe Tyr Gly Gly Arg Tyr Ser Asn Thr Lys Leu Arg Glu Gln
485 490 495
Asp Glu Tyr Val Pro Asp Phe Val Thr Leu Ser Glu Ala Leu Gly Cys
500 505 510
Val Ala Phe Arg Val Thr Arg Glu Glu Asp Val Leu Pro Thr Ile Gln
515 520 525
Lys Ala Arg Glu Ile Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val
530 535 540
Gly Glu Asp Ala Gln Val Trp Pro Met Val Ala Ala Gly Ala Ser Asn
545 550 555 560
Ser Asp Ile Glu Tyr Ala Arg Gly Leu Arg Pro Phe Phe Glu Gly Asp
565 570 575
Glu Ser Ala Gly Glu Ser Pro Ala Asp Ile His Gly Val Met Glu Asn
580 585 590
Ala Gln Glu Glu Thr Val Glu Glu Val Leu Glu Glu Arg Ala Asn Asp
595 600 605
Ser Glu Lys Glu Asn
610
<210> 99
<211> 1614
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1314)
<223> ген ilvC
<400> 99
ccgcgcccgc atcgtcgacg tggcccagga gtccgtggtc atcgaggcca ccggtacccc 60
gggcaagctg cgcgccctgc tggaggtcct cgagcccttc ggtatccgtg agctcatcca 120
gtccggtcag atcgccctca accgcggtcc gaagacgatg gcaccgtcca agtaacaccc 180
cctcatccca cctggtgaga caacacgcat gaaagtccca ccaggtgggg tacccctgtc 240
tcatgttgtg gtacaaacaa caacagcact tgtataagac gaaaggcgag tttttctccc 300
atg gct att gaa ctg ctt tac gac gcc gac gct gac ctc tcc ctc atc 348
Met Ala Ile Glu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile
1 5 10 15
cag ggc cgc aag gtc gcc atc atc ggc tac ggc tcc cag ggc cac gcc 396
Gln Gly Arg Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala
20 25 30
cac gcc cag aac ctg cgt gac tcc ggc gtc gag gtc tgc atc ggc ctg 444
His Ala Gln Asn Leu Arg Asp Ser Gly Val Glu Val Cys Ile Gly Leu
35 40 45
cgc gac ggc tcc aag tcc gcg gag aag gcc aag gag gcc ggc ttc gag 492
Arg Asp Gly Ser Lys Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu
50 55 60
gtc aag tcc aac gcc gac gcc gcc gcc tgg gca gac gtc atc atg ctg 540
Val Lys Ser Asn Ala Asp Ala Ala Ala Trp Ala Asp Val Ile Met Leu
65 70 75 80
ctc gcc ccg gac acc tcc cag aag tcc atc tac gag aac gac atc gag 588
Leu Ala Pro Asp Thr Ser Gln Lys Ser Ile Tyr Glu Asn Asp Ile Glu
85 90 95
ccg aac ctc aac gac ggt gac gcc ctg ctg ttc ggc cac ggc ctg aac 636
Pro Asn Leu Asn Asp Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn
100 105 110
gtc cac ttc ggc ctg atc aag ccg gcc gag aac atc gtc gtc ggc atg 684
Val His Phe Gly Leu Ile Lys Pro Ala Glu Asn Ile Val Val Gly Met
115 120 125
gtc gcc ccg aag ggc ccg ggc cac ctc gtc cgt cgt cag ttc gtc gac 732
Val Ala Pro Lys Gly Pro Gly His Leu Val Arg Arg Gln Phe Val Asp
130 135 140
ggc aag ggc gtc ccc tgc ctc atc gcc gtc gac cag gac ccg aag ggc 780
Gly Lys Gly Val Pro Cys Leu Ile Ala Val Asp Gln Asp Pro Lys Gly
145 150 155 160
aac ggc aag gac ctg ctg ctg tcc tac gcc gcc gcc atc ggt ggt gcc 828
Asn Gly Lys Asp Leu Leu Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala
165 170 175
cgt gca ggc gtc atc ccg acc acc ttc gag gct gag acc gtc acc gac 876
Arg Ala Gly Val Ile Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp
180 185 190
ctg ttc ggt gag cag gcc gtc ctc tgc ggt ggc acc gag gag ctc gtc 924
Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val
195 200 205
aag acc ggc ttc gag gtt ctg gtc gag gct ggc tac gag ccg gag atg 972
Lys Thr Gly Phe Glu Val Leu Val Glu Ala Gly Tyr Glu Pro Glu Met
210 215 220
gcc tac ttc gag tgc ctc cac gag ctc aag ctg atc gtt gac ctc atg 1020
Ala Tyr Phe Glu Cys Leu His Glu Leu Lys Leu Ile Val Asp Leu Met
225 230 235 240
ttc gag ggc ggc atc gcc aac atg aac tac tcc gtc tcc gac acc gcc 1068
Phe Glu Gly Gly Ile Ala Asn Met Asn Tyr Ser Val Ser Asp Thr Ala
245 250 255
gag ttc ggt ggc tac ctc tcc ggc ccg cgc gtc atc gac gcc ggc acc 1116
Glu Phe Gly Gly Tyr Leu Ser Gly Pro Arg Val Ile Asp Ala Gly Thr
260 265 270
aag gag cgc atg aag gac atc ctg acc gac atc cag gac ggc acc ttc 1164
Lys Glu Arg Met Lys Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe
275 280 285
acc aag cgc ctc atc gcc aac gtc gag aac ggc aac aag gag ctc gag 1212
Thr Lys Arg Leu Ile Ala Asn Val Glu Asn Gly Asn Lys Glu Leu Glu
290 295 300
ggt ctg cgc gag gag tac gcc aag cac cag atc gag gag acc ggc gcc 1260
Gly Leu Arg Glu Glu Tyr Ala Lys His Gln Ile Glu Glu Thr Gly Ala
305 310 315 320
aag ctg cgt gac ctc atg agc tgg gtc aag gtc gag atc acc gag acc 1308
Lys Leu Arg Asp Leu Met Ser Trp Val Lys Val Glu Ile Thr Glu Thr
325 330 335
gcc taa gcccccacgc ttgtcgacgc cccaccggtt cgccggtggg gcgttcgtct 1364
Ala
ttgccgggcc agctgagggg cctgcctcag gggtggagca ggtcgagcag gtgccgccgg 1424
agggcggggt cggggtcggc gtcgcgacgc gggcggggga ggtccacggt gatgtcggcg 1484
atgaccgtgg cgtcggggga gggggacatg acgaggatgc ggtcggagag caggatcgcc 1544
tcgtcgacgt cgtgggtgac catgacgacg gtgcggcggt cggactccca cagggagagc 1604
agctccagct 1614
<210> 100
<211> 337
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 100
Met Ala Ile Glu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile
1 5 10 15
Gln Gly Arg Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala
20 25 30
His Ala Gln Asn Leu Arg Asp Ser Gly Val Glu Val Cys Ile Gly Leu
35 40 45
Arg Asp Gly Ser Lys Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu
50 55 60
Val Lys Ser Asn Ala Asp Ala Ala Ala Trp Ala Asp Val Ile Met Leu
65 70 75 80
Leu Ala Pro Asp Thr Ser Gln Lys Ser Ile Tyr Glu Asn Asp Ile Glu
85 90 95
Pro Asn Leu Asn Asp Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn
100 105 110
Val His Phe Gly Leu Ile Lys Pro Ala Glu Asn Ile Val Val Gly Met
115 120 125
Val Ala Pro Lys Gly Pro Gly His Leu Val Arg Arg Gln Phe Val Asp
130 135 140
Gly Lys Gly Val Pro Cys Leu Ile Ala Val Asp Gln Asp Pro Lys Gly
145 150 155 160
Asn Gly Lys Asp Leu Leu Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala
165 170 175
Arg Ala Gly Val Ile Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp
180 185 190
Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val
195 200 205
Lys Thr Gly Phe Glu Val Leu Val Glu Ala Gly Tyr Glu Pro Glu Met
210 215 220
Ala Tyr Phe Glu Cys Leu His Glu Leu Lys Leu Ile Val Asp Leu Met
225 230 235 240
Phe Glu Gly Gly Ile Ala Asn Met Asn Tyr Ser Val Ser Asp Thr Ala
245 250 255
Glu Phe Gly Gly Tyr Leu Ser Gly Pro Arg Val Ile Asp Ala Gly Thr
260 265 270
Lys Glu Arg Met Lys Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe
275 280 285
Thr Lys Arg Leu Ile Ala Asn Val Glu Asn Gly Asn Lys Glu Leu Glu
290 295 300
Gly Leu Arg Glu Glu Tyr Ala Lys His Gln Ile Glu Glu Thr Gly Ala
305 310 315 320
Lys Leu Arg Asp Leu Met Ser Trp Val Lys Val Glu Ile Thr Glu Thr
325 330 335
Ala
<210> 101
<211> 2439
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2139)
<223> ген ilvD
<400> 101
gaggacgtct ccggcatcgg cttcaagggc tcccgcgccc tgctgaccac ccgcgacggc 60
accgagcacg ccatgcccgg cgtgaccttc aactccctgc cgcagctggc ggaggcctcc 120
cagggccgga tccccgatgt cctcacctcc gcccaggagg cggccgacga caaggtcacc 180
atcatccacc gcgacgggca gcagatcctc atctccaagg aggagtacgc tgctcgacag 240
gccgcgcagc aggccaaccg ctcgtcctct gacaaccctg aaaacctgaa ggagcagtaa 300
gtg ttc ccg ctt cga tcc aaa gtc acc acc gtc gga cgc aac gcc gcc 348
Val Phe Pro Leu Arg Ser Lys Val Thr Thr Val Gly Arg Asn Ala Ala
1 5 10 15
gga gcc cgc gcc ctg tgg cgc gcc acg ggc acc aag gag cac gag ttc 396
Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Thr Lys Glu His Glu Phe
20 25 30
ggt aag ccg atc gtc gcg atc gtg aac tcc tac acc cag ttc gtc ccg 444
Gly Lys Pro Ile Val Ala Ile Val Asn Ser Tyr Thr Gln Phe Val Pro
35 40 45
ggc cac gtc cac ctg aag aac gtg ggc gac atc gtc gcg gac gcc gtc 492
Gly His Val His Leu Lys Asn Val Gly Asp Ile Val Ala Asp Ala Val
50 55 60
cgt gcc gcc ggt ggc gtg ccg aag gag ttc cac acc atc gcc gtc gac 540
Arg Ala Ala Gly Gly Val Pro Lys Glu Phe His Thr Ile Ala Val Asp
65 70 75 80
gac ggc atc gcc atg gga cac ggc ggc atg ctc tac tcg ctg ccc agc 588
Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser
85 90 95
cgt gag atc atc gcc gac tct gtc gag tac atg gtc aac gcc cac acc 636
Arg Glu Ile Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Thr
100 105 110
gcc gac gcg atg gtc tgc atc tcc aac tgc gac aag atc acc ccg ggc 684
Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly
115 120 125
atg ctc aac gcc gcg ctg cgt ctc aac atc ccg gcc gtg ttc gtc tcc 732
Met Leu Asn Ala Ala Leu Arg Leu Asn Ile Pro Ala Val Phe Val Ser
130 135 140
ggc ggt ccg atg gag gcc ggc aag gcc gtc gtc gtc gac ggc gtc gcc 780
Gly Gly Pro Met Glu Ala Gly Lys Ala Val Val Val Asp Gly Val Ala
145 150 155 160
cag acc ccg acc gac ctc atc acc gcc atc gcc gcc tcc gcc gac gac 828
Gln Thr Pro Thr Asp Leu Ile Thr Ala Ile Ala Ala Ser Ala Asp Asp
165 170 175
aac gtc tcc gat gag ggc ctg gcc acc atc gag gag tcg gcc tgc ccg 876
Asn Val Ser Asp Glu Gly Leu Ala Thr Ile Glu Glu Ser Ala Cys Pro
180 185 190
acc tgt ggc tcc tgc tcc ggc atg ttc acc gcc aac tcg atg aac tgc 924
Thr Cys Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys
195 200 205
ctc acc gag gcc ctg ggc ctg tcc ctg ccg ggc aac ggc acg acg ctg 972
Leu Thr Glu Ala Leu Gly Leu Ser Leu Pro Gly Asn Gly Thr Thr Leu
210 215 220
gcc acc cac tcc gcc cgc cgt cgt ctc ttc gag cag gcc ggc gag acc 1020
Ala Thr His Ser Ala Arg Arg Arg Leu Phe Glu Gln Ala Gly Glu Thr
225 230 235 240
atc gtc gag ctg tgc agg cgc tac tac ggc gag gag gac gag tcc gtc 1068
Ile Val Glu Leu Cys Arg Arg Tyr Tyr Gly Glu Glu Asp Glu Ser Val
245 250 255
ctg ccg cgc aac atc gcc acc aag gac gcc ttc cgc aac gcg atg gcc 1116
Leu Pro Arg Asn Ile Ala Thr Lys Asp Ala Phe Arg Asn Ala Met Ala
260 265 270
ctc gac atg gcc atg ggc ggc tcc acc aac acg atc ctc cac acg ctg 1164
Leu Asp Met Ala Met Gly Gly Ser Thr Asn Thr Ile Leu His Thr Leu
275 280 285
gcc gcc gcc cag gag ggt gag gtc gac ttc acc ctg gcg gac atc gag 1212
Ala Ala Ala Gln Glu Gly Glu Val Asp Phe Thr Leu Ala Asp Ile Glu
290 295 300
gag atc tcc cac cag gtg ccg tgc atc tcc aag gtc gcc ccg aac ggc 1260
Glu Ile Ser His Gln Val Pro Cys Ile Ser Lys Val Ala Pro Asn Gly
305 310 315 320
atc tac cac atc gag gac gtc cac cgc gcc ggc ggc atc ccg gcc atc 1308
Ile Tyr His Ile Glu Asp Val His Arg Ala Gly Gly Ile Pro Ala Ile
325 330 335
ctc ggc gag ctg cgt cgc gcg aac ctg ctc aac gag gac gtc cac tcc 1356
Leu Gly Glu Leu Arg Arg Ala Asn Leu Leu Asn Glu Asp Val His Ser
340 345 350
atc acc tac gac tcc ctc gac gag tgg ctg ggt gac tgg gac atc cgt 1404
Ile Thr Tyr Asp Ser Leu Asp Glu Trp Leu Gly Asp Trp Asp Ile Arg
355 360 365
ggc ggc cgg gcc atc gac gcc gcg acc gag ctc ttc cac gcc gcc ccg 1452
Gly Gly Arg Ala Ile Asp Ala Ala Thr Glu Leu Phe His Ala Ala Pro
370 375 380
ggt ggc gtg cgc acc acg gag ccc ttc tcc acc gac aac cgc tgg gac 1500
Gly Gly Val Arg Thr Thr Glu Pro Phe Ser Thr Asp Asn Arg Trp Asp
385 390 395 400
agc ctg gac acc gac ccg gtc aac ggc tgc atc cac gac gtc gag cac 1548
Ser Leu Asp Thr Asp Pro Val Asn Gly Cys Ile His Asp Val Glu His
405 410 415
gcc cac tcc gcg gac ggc ggc ctg gtg gtc ctg cgc ggc aac ctg gcg 1596
Ala His Ser Ala Asp Gly Gly Leu Val Val Leu Arg Gly Asn Leu Ala
420 425 430
ccg gac ggc tcc gtc ctc aag acc gcc ggc gtc gag gag gag ctg tgg 1644
Pro Asp Gly Ser Val Leu Lys Thr Ala Gly Val Glu Glu Glu Leu Trp
435 440 445
acc ttc acc ggc ccg gcc cgc gtc gtc gac agc cag gag gag gcc gtc 1692
Thr Phe Thr Gly Pro Ala Arg Val Val Asp Ser Gln Glu Glu Ala Val
450 455 460
tcc atc atc ctc aag cgc gag gtc cag ccg ggt gag gtc gtc gtc atc 1740
Ser Ile Ile Leu Lys Arg Glu Val Gln Pro Gly Glu Val Val Val Ile
465 470 475 480
cgt tac gag ggt cct tcc ggt ggc ccg ggc atg cag gag atg ctg cac 1788
Arg Tyr Glu Gly Pro Ser Gly Gly Pro Gly Met Gln Glu Met Leu His
485 490 495
ccg acg tcc ttc ctc aag ggt gcc ggc ctg ggc aag aag tgc gcc ctc 1836
Pro Thr Ser Phe Leu Lys Gly Ala Gly Leu Gly Lys Lys Cys Ala Leu
500 505 510
atc acc gac ggc cgt ttc tcc ggc ggc acc tcc ggc ttg tcg atc ggc 1884
Ile Thr Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly
515 520 525
cac atc tcc ccg gag gcc gcc cac ggt ggt ctc atc ggc ctg atc cgc 1932
His Ile Ser Pro Glu Ala Ala His Gly Gly Leu Ile Gly Leu Ile Arg
530 535 540
aac ggt gac ccg atc acc atc gac gtc cac acc cgt cgt ctc tcc ctc 1980
Asn Gly Asp Pro Ile Thr Ile Asp Val His Thr Arg Arg Leu Ser Leu
545 550 555 560
gac ctg ccg gac gag gag atc gag gcc cgc cgc gcc gag atg gag gcc 2028
Asp Leu Pro Asp Glu Glu Ile Glu Ala Arg Arg Ala Glu Met Glu Ala
565 570 575
tcc gag aag ccg tgg acc gcg aac cgc gag cgc aag gtc tcc aag gca 2076
Ser Glu Lys Pro Trp Thr Ala Asn Arg Glu Arg Lys Val Ser Lys Ala
580 585 590
ctg cgt gcg tac gcc gcc atg gcc acc tcc gcc gat aag ggc gcc gtc 2124
Leu Arg Ala Tyr Ala Ala Met Ala Thr Ser Ala Asp Lys Gly Ala Val
595 600 605
cgc cag gtg gac taa cattgtccgc gtgaccacga caaacaagcc cgcgatcctg 2179
Arg Gln Val Asp
610
ccccacaccg ccatcctgag gatggtggtg gccctggggc tgatcgcggg cttcggcgtc 2239
accggacgcc agatgatgat caccgacttc cccatcgaca tgatcatcta catggagggc 2299
gtgcgggcct tcctcgacgg cgccgagatg tacgccgtgc cgatgtacgc cggctccctg 2359
gccctgccgt tcatctatcc cccgttcggc gccctcgtca tggtgccgct cacccgttac 2419
gagcatgacc tggccggcga 2439
<210> 102
<211> 612
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 102
Val Phe Pro Leu Arg Ser Lys Val Thr Thr Val Gly Arg Asn Ala Ala
1 5 10 15
Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Thr Lys Glu His Glu Phe
20 25 30
Gly Lys Pro Ile Val Ala Ile Val Asn Ser Tyr Thr Gln Phe Val Pro
35 40 45
Gly His Val His Leu Lys Asn Val Gly Asp Ile Val Ala Asp Ala Val
50 55 60
Arg Ala Ala Gly Gly Val Pro Lys Glu Phe His Thr Ile Ala Val Asp
65 70 75 80
Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser
85 90 95
Arg Glu Ile Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Thr
100 105 110
Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly
115 120 125
Met Leu Asn Ala Ala Leu Arg Leu Asn Ile Pro Ala Val Phe Val Ser
130 135 140
Gly Gly Pro Met Glu Ala Gly Lys Ala Val Val Val Asp Gly Val Ala
145 150 155 160
Gln Thr Pro Thr Asp Leu Ile Thr Ala Ile Ala Ala Ser Ala Asp Asp
165 170 175
Asn Val Ser Asp Glu Gly Leu Ala Thr Ile Glu Glu Ser Ala Cys Pro
180 185 190
Thr Cys Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys
195 200 205
Leu Thr Glu Ala Leu Gly Leu Ser Leu Pro Gly Asn Gly Thr Thr Leu
210 215 220
Ala Thr His Ser Ala Arg Arg Arg Leu Phe Glu Gln Ala Gly Glu Thr
225 230 235 240
Ile Val Glu Leu Cys Arg Arg Tyr Tyr Gly Glu Glu Asp Glu Ser Val
245 250 255
Leu Pro Arg Asn Ile Ala Thr Lys Asp Ala Phe Arg Asn Ala Met Ala
260 265 270
Leu Asp Met Ala Met Gly Gly Ser Thr Asn Thr Ile Leu His Thr Leu
275 280 285
Ala Ala Ala Gln Glu Gly Glu Val Asp Phe Thr Leu Ala Asp Ile Glu
290 295 300
Glu Ile Ser His Gln Val Pro Cys Ile Ser Lys Val Ala Pro Asn Gly
305 310 315 320
Ile Tyr His Ile Glu Asp Val His Arg Ala Gly Gly Ile Pro Ala Ile
325 330 335
Leu Gly Glu Leu Arg Arg Ala Asn Leu Leu Asn Glu Asp Val His Ser
340 345 350
Ile Thr Tyr Asp Ser Leu Asp Glu Trp Leu Gly Asp Trp Asp Ile Arg
355 360 365
Gly Gly Arg Ala Ile Asp Ala Ala Thr Glu Leu Phe His Ala Ala Pro
370 375 380
Gly Gly Val Arg Thr Thr Glu Pro Phe Ser Thr Asp Asn Arg Trp Asp
385 390 395 400
Ser Leu Asp Thr Asp Pro Val Asn Gly Cys Ile His Asp Val Glu His
405 410 415
Ala His Ser Ala Asp Gly Gly Leu Val Val Leu Arg Gly Asn Leu Ala
420 425 430
Pro Asp Gly Ser Val Leu Lys Thr Ala Gly Val Glu Glu Glu Leu Trp
435 440 445
Thr Phe Thr Gly Pro Ala Arg Val Val Asp Ser Gln Glu Glu Ala Val
450 455 460
Ser Ile Ile Leu Lys Arg Glu Val Gln Pro Gly Glu Val Val Val Ile
465 470 475 480
Arg Tyr Glu Gly Pro Ser Gly Gly Pro Gly Met Gln Glu Met Leu His
485 490 495
Pro Thr Ser Phe Leu Lys Gly Ala Gly Leu Gly Lys Lys Cys Ala Leu
500 505 510
Ile Thr Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly
515 520 525
His Ile Ser Pro Glu Ala Ala His Gly Gly Leu Ile Gly Leu Ile Arg
530 535 540
Asn Gly Asp Pro Ile Thr Ile Asp Val His Thr Arg Arg Leu Ser Leu
545 550 555 560
Asp Leu Pro Asp Glu Glu Ile Glu Ala Arg Arg Ala Glu Met Glu Ala
565 570 575
Ser Glu Lys Pro Trp Thr Ala Asn Arg Glu Arg Lys Val Ser Lys Ala
580 585 590
Leu Arg Ala Tyr Ala Ala Met Ala Thr Ser Ala Asp Lys Gly Ala Val
595 600 605
Arg Gln Val Asp
610
<210> 103
<211> 1725
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1425)
<223> ген ilvE
<400> 103
gatctcgttg gccttgcccg tggcaccgac ggcggtgagg gcgtcgagga cctcgcggga 60
ggcggcgtcg tcgagaagga cggaggccgg cagctccggg ccgtcctcac cgcagaggac 120
ggggacgagc agtgcgtcgg gggtcttcgg ggccttcttg gccagctcga cggcgggggt 180
ggtgccgcgg gcgggaaggg tgaaagtggg gttcgccatg gaaagtacct cctgagattg 240
acaagcgatg aacccagctt atagagatca cctctgggtc gtcggtgtag cgtgttcccc 300
atg acg tct ctc aat ttc acc gtg aac cgt acg agc acc ccg acg tcc 348
Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser
1 5 10 15
gat gag gcg cgc gag gag att ctg cag aat ccg cgg ttc ggc aag aac 396
Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn
20 25 30
ttc acc gat cac atg gtc acc atc gag tgg tcc gag gac aag ggc tgg 444
Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp
35 40 45
cac gac gcc cgc gtg cgt ccc tac gag gcc atc ccg atg gat ccc gcg 492
His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala
50 55 60
acc acg gtg ttc cac tac ggc cag gcg atc ttc gag ggc atc aag gcc 540
Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala
65 70 75 80
tac cgt cag ccg gac ggc tcc atc gcc acc ttc cgc ccg gag cgc aac 588
Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn
85 90 95
gcc gag cgc atg cag cgt tcc gcc gag cgc atg gcc atg ccg ccg ctg 636
Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu
100 105 110
ccg acc gag gac ttc ctc gag acg ctc cgc ctg ctt gtc gac gtc gac 684
Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp
115 120 125
cgt gac tgg gtc ccc gcc gcc ggc ggc gag gcc agc ctc tac ctg cgt 732
Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg
130 135 140
ccc ttc atg atc tcc acc gag gtc tcc ctg ggc gtc agc ccg gcc aac 780
Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn
145 150 155 160
cgg tac acc ttc ttc gtc atc gcc tcc ccg gtc ggg gcg tac ttc acc 828
Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr
165 170 175
ggt ggc gtc aag ccg gtc tcc gtg tgg ctg agc gag gac tac gtc cgt 876
Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg
180 185 190
gcc gcc ccg ggt ggc acc ggt gcc gcg aag ttc gcc ggc aac tac gcc 924
Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala
195 200 205
gcc tcc ctg ctc gcg cag gcg cag gcg gag gag aag ggc tgc gac cag 972
Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln
210 215 220
gtc gtg tgg ctc gac gcc atc gag cac acc tac atc gag gag atg ggt 1020
Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly
225 230 235 240
ggc atg aac ctg ttc ttc gtg tac ggc tcg ggt gac gac gtc acc gtg 1068
Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val
245 250 255
gcc acc ccc gag ctc tcc ggc tcc ctc ctg ccg ggc gtc acc cgc gac 1116
Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp
260 265 270
tcc ctc ctg cag gtc gcc cgc gat ctc ggc cac cgc acc gag gag cgg 1164
Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg
275 280 285
agg atc tcc aag ttc gac tgg cgc gac gac gtc gcc tcc ggt gcg atg 1212
Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met
290 295 300
acc gag gcc atg gcc tgc ggc aca gcc gcc gtg atc acc ccg gtc ggc 1260
Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly
305 310 315 320
cgg gtc ctg tcc aac gac ggc gag ttc cag atc aat gac aac cag ccg 1308
Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro
325 330 335
ggt gag gtc acc atg gcc atg cgc gag cgt ctc acc gac atc cag cgc 1356
Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg
340 345 350
ggc ctg tac gag gac acc cac ggc tgg gtg cac acc ctc gtc ccg gct 1404
Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala
355 360 365
gag gac cag ccg gcg gac tag tcggccagca gctcggcggc ggcccgcacc 1455
Glu Asp Gln Pro Ala Asp
370
agcgggagcg ccgctagcgc gccggtgccc tggccggcgt tcatgtccag ggcgacgaga 1515
ggcgtgagtt cgagggcctg cagcgcgatg aggtgcgcgg gctccggggt cagctgccct 1575
gcctggcacc agggggcggt gccgggggag agacgttccg cgaggtaggc ggcgacggtg 1635
acgggggcgc cgtcgagaag cacgggggtg cggcgtgccg cggcctgcgc gatgaaggcc 1695
accagtgcca ccaggtccgg ggaggcgatc 1725
<210> 104
<211> 374
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 104
Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser
1 5 10 15
Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn
20 25 30
Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp
35 40 45
His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala
50 55 60
Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala
65 70 75 80
Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn
85 90 95
Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu
100 105 110
Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp
115 120 125
Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg
130 135 140
Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn
145 150 155 160
Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr
165 170 175
Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg
180 185 190
Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala
195 200 205
Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln
210 215 220
Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly
225 230 235 240
Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val
245 250 255
Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp
260 265 270
Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg
275 280 285
Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met
290 295 300
Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly
305 310 315 320
Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro
325 330 335
Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg
340 345 350
Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala
355 360 365
Glu Asp Gln Pro Ala Asp
370
<210> 105
<211> 1896
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1596)
<223> ген ilvA
<400> 105
gccgatcggg gccagcgaga gggccgcacc cggggccgag tggacgacgg ggttgtccac 60
gacagcggcg tgggccgggg cgatgaggga gacactggtg gcgagcgccg cgcacagcgc 120
gacagcggaa cggcgaaggg acacggggag aactcctgtg gtcggtgggg tgcagatcgt 180
gtgacatggt ggaccagtga catgacacca atctcaattc cggaccacct gcaggtgaac 240
agtcggtgaa gtgggctgca acgccgtggc cggtggacag ggggactaca ctgagtcccc 300
atg agt gac acc cgg tct gcc gta acc acc ttc gag cct gtc cgc gcc 348
Met Ser Asp Thr Arg Ser Ala Val Thr Thr Phe Glu Pro Val Arg Ala
1 5 10 15
tcg gac att cag ctg gcg cag gca cgg atc tcc tcc gtc atc gag ccc 396
Ser Asp Ile Gln Leu Ala Gln Ala Arg Ile Ser Ser Val Ile Glu Pro
20 25 30
acc ccg ctc cag tac tgc ccc cgc ctc tcc gag gag acc ggc tgc gag 444
Thr Pro Leu Gln Tyr Cys Pro Arg Leu Ser Glu Glu Thr Gly Cys Glu
35 40 45
gtg tac ctc aaa cgc gag gac ctg cag gac gtg cgc tcc tac aag atc 492
Val Tyr Leu Lys Arg Glu Asp Leu Gln Asp Val Arg Ser Tyr Lys Ile
50 55 60
cgc ggc gcc tac tac gcc atg gcc aac ctc gac gag cag cag cgg gac 540
Arg Gly Ala Tyr Tyr Ala Met Ala Asn Leu Asp Glu Gln Gln Arg Asp
65 70 75 80
gcc ggc atc gtc gcc gcc tcc gcc ggc aac cac gca cag ggt gtc gcc 588
Ala Gly Ile Val Ala Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala
85 90 95
tac gcc tgc cgc acg atg ggc atc cgg ggt cgc atc ttc gtc ccc aac 636
Tyr Ala Cys Arg Thr Met Gly Ile Arg Gly Arg Ile Phe Val Pro Asn
100 105 110
cag acg ccg aag cag aag cgt gac cgc atc atg gtc cac ggc ggt gac 684
Gln Thr Pro Lys Gln Lys Arg Asp Arg Ile Met Val His Gly Gly Asp
115 120 125
atg gtg gag ctg gtc gtc acc ggc aac aac ttc gac gag gcc gcc gag 732
Met Val Glu Leu Val Val Thr Gly Asn Asn Phe Asp Glu Ala Ala Glu
130 135 140
gcc gcg cgt gcc gac gcc gcc gag cgc ggc gcc acc atg atc gag ccc 780
Ala Ala Arg Ala Asp Ala Ala Glu Arg Gly Ala Thr Met Ile Glu Pro
145 150 155 160
ttc gac gcc cgc gac acc gtc atc ggt cag ggg acc atc gcg gcg gag 828
Phe Asp Ala Arg Asp Thr Val Ile Gly Gln Gly Thr Ile Ala Ala Glu
165 170 175
atc ctc acg cag ctc acg tcc atc ggc aag tcc ctc gac acg gtg ctc 876
Ile Leu Thr Gln Leu Thr Ser Ile Gly Lys Ser Leu Asp Thr Val Leu
180 185 190
gtg ccg gtc ggc ggc ggt ggc ctc ctg gcc ggt gtc gcc agc tac ctc 924
Val Pro Val Gly Gly Gly Gly Leu Leu Ala Gly Val Ala Ser Tyr Leu
195 200 205
gcc gac atg gcg ccg cgc acc gcg atc gtc ggc gtc gag ccg ggc ggt 972
Ala Asp Met Ala Pro Arg Thr Ala Ile Val Gly Val Glu Pro Gly Gly
210 215 220
gcc gcc tcc ctc cag gcc gcc ctg cgt gcc gac gcc ccc gtc acc ctc 1020
Ala Ala Ser Leu Gln Ala Ala Leu Arg Ala Asp Ala Pro Val Thr Leu
225 230 235 240
tcc gcg gtc gac ccc ttc gtc gac ggc gcc gcc gtc aag cgc atc ggt 1068
Ser Ala Val Asp Pro Phe Val Asp Gly Ala Ala Val Lys Arg Ile Gly
245 250 255
gac ctg ccc ttc gac atc atc gaa cgc aac aag ggc cgc atc cac gtc 1116
Asp Leu Pro Phe Asp Ile Ile Glu Arg Asn Lys Gly Arg Ile His Val
260 265 270
atg gac gtc tcc gag ggc gcc gtg tgc acc gag atg ctc gac ctg tac 1164
Met Asp Val Ser Glu Gly Ala Val Cys Thr Glu Met Leu Asp Leu Tyr
275 280 285
cag aac gag ggc atc atc acc gag ccc gcc ggt gca ctg tcg gtc acg 1212
Gln Asn Glu Gly Ile Ile Thr Glu Pro Ala Gly Ala Leu Ser Val Thr
290 295 300
ggc ctg aag gag atg aag ctc cag cag gac tcc atc gtg gtg tgc atc 1260
Gly Leu Lys Glu Met Lys Leu Gln Gln Asp Ser Ile Val Val Cys Ile
305 310 315 320
atc tcc ggc ggc aac aac gac gtg ctg cgc tac gcc gag atc atg gag 1308
Ile Ser Gly Gly Asn Asn Asp Val Leu Arg Tyr Ala Glu Ile Met Glu
325 330 335
cgc tcc ctg gtg cac cgc ggc ctc aag cac tac ttc ctg gtg aac ttc 1356
Arg Ser Leu Val His Arg Gly Leu Lys His Tyr Phe Leu Val Asn Phe
340 345 350
ccg cag gag ccg ggc cag ctg cgc cac ttc ctc aac gac atc ctc ggc 1404
Pro Gln Glu Pro Gly Gln Leu Arg His Phe Leu Asn Asp Ile Leu Gly
355 360 365
ccc gac gac gac atc acc ctc ttc gag tac ctc aag cgc aac aac cgg 1452
Pro Asp Asp Asp Ile Thr Leu Phe Glu Tyr Leu Lys Arg Asn Asn Arg
370 375 380
gag acc ggt gcc gcg ctc gtc ggc ctc gag ctc ggc cgg gcc agc gac 1500
Glu Thr Gly Ala Ala Leu Val Gly Leu Glu Leu Gly Arg Ala Ser Asp
385 390 395 400
ctc gac ccg ctg ctc gag cgg atg aac gcc tcc cgc atc gag tgc cag 1548
Leu Asp Pro Leu Leu Glu Arg Met Asn Ala Ser Arg Ile Glu Cys Gln
405 410 415
cac ctc aag ccg aac acc ccc gag tac gag tac ctg gtc aac acc tag 1596
His Leu Lys Pro Asn Thr Pro Glu Tyr Glu Tyr Leu Val Asn Thr
420 425 430
gggtctctac ctgcgcagga gcgcgaactc ccacgggccc aggcgcgtgt cgtcgaggcc 1656
gacctccggt tccgtgaagg agtagatgag ctcgccgccg tagggggcgc ggacctccgc 1716
gtcggagagg ttggccacga ggatcacgtc ctcccagccc atcgccagcc agccgtcccc 1776
ggtctcgacg gtgaggcggg agagatcggc gcgggagagg ccgagctcgc ggcgcagacg 1836
cagcagctcc cggtaggcgg cgtggatctc ctgctgctcc tccgtgaagt cccagtccag 1896
<210> 106
<211> 431
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 106
Met Ser Asp Thr Arg Ser Ala Val Thr Thr Phe Glu Pro Val Arg Ala
1 5 10 15
Ser Asp Ile Gln Leu Ala Gln Ala Arg Ile Ser Ser Val Ile Glu Pro
20 25 30
Thr Pro Leu Gln Tyr Cys Pro Arg Leu Ser Glu Glu Thr Gly Cys Glu
35 40 45
Val Tyr Leu Lys Arg Glu Asp Leu Gln Asp Val Arg Ser Tyr Lys Ile
50 55 60
Arg Gly Ala Tyr Tyr Ala Met Ala Asn Leu Asp Glu Gln Gln Arg Asp
65 70 75 80
Ala Gly Ile Val Ala Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala
85 90 95
Tyr Ala Cys Arg Thr Met Gly Ile Arg Gly Arg Ile Phe Val Pro Asn
100 105 110
Gln Thr Pro Lys Gln Lys Arg Asp Arg Ile Met Val His Gly Gly Asp
115 120 125
Met Val Glu Leu Val Val Thr Gly Asn Asn Phe Asp Glu Ala Ala Glu
130 135 140
Ala Ala Arg Ala Asp Ala Ala Glu Arg Gly Ala Thr Met Ile Glu Pro
145 150 155 160
Phe Asp Ala Arg Asp Thr Val Ile Gly Gln Gly Thr Ile Ala Ala Glu
165 170 175
Ile Leu Thr Gln Leu Thr Ser Ile Gly Lys Ser Leu Asp Thr Val Leu
180 185 190
Val Pro Val Gly Gly Gly Gly Leu Leu Ala Gly Val Ala Ser Tyr Leu
195 200 205
Ala Asp Met Ala Pro Arg Thr Ala Ile Val Gly Val Glu Pro Gly Gly
210 215 220
Ala Ala Ser Leu Gln Ala Ala Leu Arg Ala Asp Ala Pro Val Thr Leu
225 230 235 240
Ser Ala Val Asp Pro Phe Val Asp Gly Ala Ala Val Lys Arg Ile Gly
245 250 255
Asp Leu Pro Phe Asp Ile Ile Glu Arg Asn Lys Gly Arg Ile His Val
260 265 270
Met Asp Val Ser Glu Gly Ala Val Cys Thr Glu Met Leu Asp Leu Tyr
275 280 285
Gln Asn Glu Gly Ile Ile Thr Glu Pro Ala Gly Ala Leu Ser Val Thr
290 295 300
Gly Leu Lys Glu Met Lys Leu Gln Gln Asp Ser Ile Val Val Cys Ile
305 310 315 320
Ile Ser Gly Gly Asn Asn Asp Val Leu Arg Tyr Ala Glu Ile Met Glu
325 330 335
Arg Ser Leu Val His Arg Gly Leu Lys His Tyr Phe Leu Val Asn Phe
340 345 350
Pro Gln Glu Pro Gly Gln Leu Arg His Phe Leu Asn Asp Ile Leu Gly
355 360 365
Pro Asp Asp Asp Ile Thr Leu Phe Glu Tyr Leu Lys Arg Asn Asn Arg
370 375 380
Glu Thr Gly Ala Ala Leu Val Gly Leu Glu Leu Gly Arg Ala Ser Asp
385 390 395 400
Leu Asp Pro Leu Leu Glu Arg Met Asn Ala Ser Arg Ile Glu Cys Gln
405 410 415
His Leu Lys Pro Asn Thr Pro Glu Tyr Glu Tyr Leu Val Asn Thr
420 425 430
<210> 107
<211> 2043
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1743)
<223> ген thrC
<400> 107
cggggcagca ccgcgatggc cacgacgagg acgagcagga ccgcggtgac ggtggctacg 60
gtacgtgtgt tacctcggga ggccacgggg cgctcactcc tctgtgcgac tctctgcgac 120
tctgtacgac tctgtgcgac gcggcgtgga caacgccgcc cggcaggggc gtgcggccgc 180
agtaccccgt agcgtactca actgcttttc ccgggaaagg ggaattcacc gggatccccg 240
ccccggcgtg cgggtgggat gttgcgcatg cttccagccc cgccacgtat ggttgtccac 300
gtg gat tac att tcg acg cgc gac tcc tcc cgt act ccc gcc acc ttc 348
Val Asp Tyr Ile Ser Thr Arg Asp Ser Ser Arg Thr Pro Ala Thr Phe
1 5 10 15
acc gac atc ctg ctc ggt gga ctg gcc ccc gac ggc ggc ctc tac ctg 396
Thr Asp Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu
20 25 30
ccc gcc gag tac ccg cag atc agc gat gag cag ctg acg cag tgg cgg 444
Pro Ala Glu Tyr Pro Gln Ile Ser Asp Glu Gln Leu Thr Gln Trp Arg
35 40 45
tct ctc ctg cag gag aag ggg tac gcc gcc ctg gcg gcg gag atc ctc 492
Ser Leu Leu Gln Glu Lys Gly Tyr Ala Ala Leu Ala Ala Glu Ile Leu
50 55 60
aag ctc ttc gtc gac gac atc ccg gcg gag gat ctc gag ggc atc gcc 540
Lys Leu Phe Val Asp Asp Ile Pro Ala Glu Asp Leu Glu Gly Ile Ala
65 70 75 80
gcc cgc gcc tac acc tcc ccg aag ttc gcg cac gag gag atc gtc ccc 588
Ala Arg Ala Tyr Thr Ser Pro Lys Phe Ala His Glu Glu Ile Val Pro
85 90 95
gtg acc gag ctc gag gac ggc ctg tgg atc ggc cac ctc tcc gag ggc 636
Val Thr Glu Leu Glu Asp Gly Leu Trp Ile Gly His Leu Ser Glu Gly
100 105 110
ccg acc gcc gcg ttc aag gac atg gcg atg cag ctg ctc ggc gag ctc 684
Pro Thr Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu
115 120 125
ttc gag tac gag ctg gcc cgt cgc ggg gag acc ctc aac atc ctg ggt 732
Phe Glu Tyr Glu Leu Ala Arg Arg Gly Glu Thr Leu Asn Ile Leu Gly
130 135 140
gcg acc tcg ggt gac acg ggt tcc tcc gcc gag tac gcg atg cgc ggc 780
Ala Thr Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly
145 150 155 160
cgt aag ggc atc cgt gtc ttc atg ctg acc ccg gcc ggt cgc atg acc 828
Arg Lys Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr
165 170 175
ccg ttc cag cag gcg cag atg ttc ggc ctg cag gac ccc aac atc ttc 876
Pro Phe Gln Gln Ala Gln Met Phe Gly Leu Gln Asp Pro Asn Ile Phe
180 185 190
aac atc gcc ctg gac ggc gtc ttc gac gac tgc cag gac gtg gtc aag 924
Asn Ile Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys
195 200 205
gcg gtg tcc gcc gac gcc ggg ttc aag gcc gag aac cgt atc ggt gcg 972
Ala Val Ser Ala Asp Ala Gly Phe Lys Ala Glu Asn Arg Ile Gly Ala
210 215 220
gtc aac tcg atc aac tgg gcc cgc ctc atg gcg cag atc gtc tac tac 1020
Val Asn Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Ile Val Tyr Tyr
225 230 235 240
gtc tcc tcc tgg ctg agg atc acc gag agc aac gac cag aag gtc tcc 1068
Val Ser Ser Trp Leu Arg Ile Thr Glu Ser Asn Asp Gln Lys Val Ser
245 250 255
ttc tcc gtg ccg acc ggc aac ttc ggt gac atc tgc gcc ggc cac atc 1116
Phe Ser Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile
260 265 270
gcc cgt cag atg ggt ctg ccg atc gac cgt ctc atc gtc gcc acc aac 1164
Ala Arg Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn
275 280 285
gag aac gac gtc ctc gac gag ttc ttc cgc acc ggc aac tac cgt ccg 1212
Glu Asn Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asn Tyr Arg Pro
290 295 300
cgc ccg gcg gag gag acc ctg gag acg tcc tcg ccg tcg atg gac atc 1260
Arg Pro Ala Glu Glu Thr Leu Glu Thr Ser Ser Pro Ser Met Asp Ile
305 310 315 320
tcc cgt gcc tcc aac ttc gag cgt ttc gtc ttc gac ctg ctg ggc cgt 1308
Ser Arg Ala Ser Asn Phe Glu Arg Phe Val Phe Asp Leu Leu Gly Arg
325 330 335
gac gcc gcg cgc gtc gcg cag cac ttc ggc gtc gac gtc cgc gcc gac 1356
Asp Ala Ala Arg Val Ala Gln His Phe Gly Val Asp Val Arg Ala Asp
340 345 350
ggt ttc tcg ctt gcc gac gac ccc gcg ttc ccg gcc gcc gca gag aag 1404
Gly Phe Ser Leu Ala Asp Asp Pro Ala Phe Pro Ala Ala Ala Glu Lys
355 360 365
tac ggc ttc gcc tcc ggt cgt tcc acc cac cag gac cgc ctc gac acg 1452
Tyr Gly Phe Ala Ser Gly Arg Ser Thr His Gln Asp Arg Leu Asp Thr
370 375 380
atc agg gac gtg tgg gag cgc ctg ggc atc atg ctc gac ccg cac acc 1500
Ile Arg Asp Val Trp Glu Arg Leu Gly Ile Met Leu Asp Pro His Thr
385 390 395 400
gcc gac ggc gtg aag gtc gcc cgc gag tgg cgt tcc gag atc gac ggc 1548
Ala Asp Gly Val Lys Val Ala Arg Glu Trp Arg Ser Glu Ile Asp Gly
405 410 415
ccg atc gtg tgc ctg gag acc gcc ctg ccg gtg aag ttc tcc gag acc 1596
Pro Ile Val Cys Leu Glu Thr Ala Leu Pro Val Lys Phe Ser Glu Thr
420 425 430
atc gag gag gcc acc ggc gtg cag ccg gag acc ccg gag cgt ttc gcc 1644
Ile Glu Glu Ala Thr Gly Val Gln Pro Glu Thr Pro Glu Arg Phe Ala
435 440 445
ggc atc ctc gac gcc gag cgc cac gtg acg gac ctg ccg aac gac gcg 1692
Gly Ile Leu Asp Ala Glu Arg His Val Thr Asp Leu Pro Asn Asp Ala
450 455 460
gag acc gtc aag aag ttc atc acc gac tcc atc cgc acc acc gag gtg 1740
Glu Thr Val Lys Lys Phe Ile Thr Asp Ser Ile Arg Thr Thr Glu Val
465 470 475 480
taa ccgtcgtggc catggagttc aagatgggtc aggagtactt ccaggacttc 1793
gacccggaga agttcgccgc gatgatcaag gcgcaggccg aggctgaggc tgacgctgcg 1853
gccggggaag acggcgtcga caagcagtga acatccgcgt ctgggcgggt ctggtcgcac 1913
tgaccaccgc cggcgtgggg tcgctcatcg cctggtccgt cgaggaccag cggcagcacc 1973
ccacgcccat cgccgaggtg atcgcggcgg cgccgacgca ggtcccgacg gagttccgtg 2033
cccctgcggt 2043
<210> 108
<211> 480
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 108
Val Asp Tyr Ile Ser Thr Arg Asp Ser Ser Arg Thr Pro Ala Thr Phe
1 5 10 15
Thr Asp Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu
20 25 30
Pro Ala Glu Tyr Pro Gln Ile Ser Asp Glu Gln Leu Thr Gln Trp Arg
35 40 45
Ser Leu Leu Gln Glu Lys Gly Tyr Ala Ala Leu Ala Ala Glu Ile Leu
50 55 60
Lys Leu Phe Val Asp Asp Ile Pro Ala Glu Asp Leu Glu Gly Ile Ala
65 70 75 80
Ala Arg Ala Tyr Thr Ser Pro Lys Phe Ala His Glu Glu Ile Val Pro
85 90 95
Val Thr Glu Leu Glu Asp Gly Leu Trp Ile Gly His Leu Ser Glu Gly
100 105 110
Pro Thr Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu
115 120 125
Phe Glu Tyr Glu Leu Ala Arg Arg Gly Glu Thr Leu Asn Ile Leu Gly
130 135 140
Ala Thr Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly
145 150 155 160
Arg Lys Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr
165 170 175
Pro Phe Gln Gln Ala Gln Met Phe Gly Leu Gln Asp Pro Asn Ile Phe
180 185 190
Asn Ile Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys
195 200 205
Ala Val Ser Ala Asp Ala Gly Phe Lys Ala Glu Asn Arg Ile Gly Ala
210 215 220
Val Asn Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Ile Val Tyr Tyr
225 230 235 240
Val Ser Ser Trp Leu Arg Ile Thr Glu Ser Asn Asp Gln Lys Val Ser
245 250 255
Phe Ser Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile
260 265 270
Ala Arg Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn
275 280 285
Glu Asn Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asn Tyr Arg Pro
290 295 300
Arg Pro Ala Glu Glu Thr Leu Glu Thr Ser Ser Pro Ser Met Asp Ile
305 310 315 320
Ser Arg Ala Ser Asn Phe Glu Arg Phe Val Phe Asp Leu Leu Gly Arg
325 330 335
Asp Ala Ala Arg Val Ala Gln His Phe Gly Val Asp Val Arg Ala Asp
340 345 350
Gly Phe Ser Leu Ala Asp Asp Pro Ala Phe Pro Ala Ala Ala Glu Lys
355 360 365
Tyr Gly Phe Ala Ser Gly Arg Ser Thr His Gln Asp Arg Leu Asp Thr
370 375 380
Ile Arg Asp Val Trp Glu Arg Leu Gly Ile Met Leu Asp Pro His Thr
385 390 395 400
Ala Asp Gly Val Lys Val Ala Arg Glu Trp Arg Ser Glu Ile Asp Gly
405 410 415
Pro Ile Val Cys Leu Glu Thr Ala Leu Pro Val Lys Phe Ser Glu Thr
420 425 430
Ile Glu Glu Ala Thr Gly Val Gln Pro Glu Thr Pro Glu Arg Phe Ala
435 440 445
Gly Ile Leu Asp Ala Glu Arg His Val Thr Asp Leu Pro Asn Asp Ala
450 455 460
Glu Thr Val Lys Lys Phe Ile Thr Asp Ser Ile Arg Thr Thr Glu Val
465 470 475 480
<210> 109
<211> 2448
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2148)
<223> ген leuA
<400> 109
gggtcgatgc cgtggagcag ggcttttcac gtttcttcac aaaactgcgt tgcgccgggg 60
gagggagtgt gtagacttca cagcatgttc tcccgcgcga acctccttct tcttcgccgc 120
ggcgggtccc aggtccagta acgaagcgac cggcaccccg tcgcggagtt tggtgttgcc 180
ggtcgctgct cccgctgaat agcccattca tcagcggtga gacctcgcag gctactacag 240
tgaaggccgt tgtcggcccc tggtagtccg cagccacaga cagacccgaa aaggaaactc 300
atg tcc ccg aac gac tcc ttc atc tcc gcc ccc tcc gag atc cgc acc 348
Met Ser Pro Asn Asp Ser Phe Ile Ser Ala Pro Ser Glu Ile Arg Thr
1 5 10 15
ccg gac gga ccg cgc cgc gag ggc cag ccc ttc tgg aac aag cag cgc 396
Pro Asp Gly Pro Arg Arg Glu Gly Gln Pro Phe Trp Asn Lys Gln Arg
20 25 30
aac tcc tcc atg ccg gta cac cgc tac cgc ccc ttc gcc gag gcc gtc 444
Asn Ser Ser Met Pro Val His Arg Tyr Arg Pro Phe Ala Glu Ala Val
35 40 45
gag gac atc gtt ctg ccg gac cgc acc tgg ccg gac aag aag atc acc 492
Glu Asp Ile Val Leu Pro Asp Arg Thr Trp Pro Asp Lys Lys Ile Thr
50 55 60
gcc gcc ccg cag tgg tgt gcg gtc gac ctg cgt gac ggc aac cag gcg 540
Ala Ala Pro Gln Trp Cys Ala Val Asp Leu Arg Asp Gly Asn Gln Ala
65 70 75 80
ctc atc gac ccg atg agc ccg gaa cgc aag cgc cgc atg ttc aac ctg 588
Leu Ile Asp Pro Met Ser Pro Glu Arg Lys Arg Arg Met Phe Asn Leu
85 90 95
ctg gtg cag atg ggc tac aag gag atc gag gtt ggt ttc ccg tcc gcc 636
Leu Val Gln Met Gly Tyr Lys Glu Ile Glu Val Gly Phe Pro Ser Ala
100 105 110
tcc cag acc gac ttc gac ttc gtc cgc gag atc atc gag cag gac atg 684
Ser Gln Thr Asp Phe Asp Phe Val Arg Glu Ile Ile Glu Gln Asp Met
115 120 125
atc ccg gag gac gtc acc atc cag gtc ctg gtc cag gcc cgt gag cac 732
Ile Pro Glu Asp Val Thr Ile Gln Val Leu Val Gln Ala Arg Glu His
130 135 140
ctg atc cgc cgc acc ttc gag gcg tgc gag ggc gcg aag aac gtc atc 780
Leu Ile Arg Arg Thr Phe Glu Ala Cys Glu Gly Ala Lys Asn Val Ile
145 150 155 160
gtc cac ttc tac aac tcc acc tcc gag ctg cag cgc cgc gtc gtg ttc 828
Val His Phe Tyr Asn Ser Thr Ser Glu Leu Gln Arg Arg Val Val Phe
165 170 175
cgc aag gat aag ccg gcc atc aag cag ctg gcc gtc gac gcc gcc gag 876
Arg Lys Asp Lys Pro Ala Ile Lys Gln Leu Ala Val Asp Ala Ala Glu
180 185 190
ctg atc cag acc atc gcc cag gac tac ccg ggc acc aac tgg cgt tgg 924
Leu Ile Gln Thr Ile Ala Gln Asp Tyr Pro Gly Thr Asn Trp Arg Trp
195 200 205
cag tac tcc ccg gag tcc ttc acc ggc acc gag ctg gag ttc gcc ctc 972
Gln Tyr Ser Pro Glu Ser Phe Thr Gly Thr Glu Leu Glu Phe Ala Leu
210 215 220
gag gtc tgc aac gcc gtc atc gag gtc atg gcg ccc acc ccg gac aac 1020
Glu Val Cys Asn Ala Val Ile Glu Val Met Ala Pro Thr Pro Asp Asn
225 230 235 240
ccg atc aac atc aac ctg ccg tcc acc gtc gag atg atc acc ccg aac 1068
Pro Ile Asn Ile Asn Leu Pro Ser Thr Val Glu Met Ile Thr Pro Asn
245 250 255
gtc tac gcc gac tcc atc gag tgg atg cac cgc aac ctg gac cgc cgt 1116
Val Tyr Ala Asp Ser Ile Glu Trp Met His Arg Asn Leu Asp Arg Arg
260 265 270
gac tcc gtc atc ctg tcg ctg cac ccc cac aac gac cgt ggt gag ggc 1164
Asp Ser Val Ile Leu Ser Leu His Pro His Asn Asp Arg Gly Glu Gly
275 280 285
gtc gcc gcc gcc gag ctg ggc tac ctg gcc ggc gcc gac cgc atc gag 1212
Val Ala Ala Ala Glu Leu Gly Tyr Leu Ala Gly Ala Asp Arg Ile Glu
290 295 300
ggc tgc ctg ttc ggc aac ggt gag cgc acc ggt aac gtc tgc ctg gtc 1260
Gly Cys Leu Phe Gly Asn Gly Glu Arg Thr Gly Asn Val Cys Leu Val
305 310 315 320
acc ctg ggc ctg aac atg ctc acc cag ggc gtc gac ccg cag atc gac 1308
Thr Leu Gly Leu Asn Met Leu Thr Gln Gly Val Asp Pro Gln Ile Asp
325 330 335
ttc tcc gac atc cgc cag atc cgc agc acc gtc gag tac tgc aac cag 1356
Phe Ser Asp Ile Arg Gln Ile Arg Ser Thr Val Glu Tyr Cys Asn Gln
340 345 350
ctg cga gtc ccg gag cgt cac ccc tac ggc ggc gac ctg gtc ttc acc 1404
Leu Arg Val Pro Glu Arg His Pro Tyr Gly Gly Asp Leu Val Phe Thr
355 360 365
gcc ttc tcc ggc tcc cac cag gac gcc gtc aac aag ggc ctc gac gcc 1452
Ala Phe Ser Gly Ser His Gln Asp Ala Val Asn Lys Gly Leu Asp Ala
370 375 380
atg gcc gcc aag gtc aag ccg ggc gcc aac aac acc gag gtc acc tgg 1500
Met Ala Ala Lys Val Lys Pro Gly Ala Asn Asn Thr Glu Val Thr Trp
385 390 395 400
gag gag ctg ggc aag gcc gtc tgg gag gtt ccc tac ctg ccc atc gac 1548
Glu Glu Leu Gly Lys Ala Val Trp Glu Val Pro Tyr Leu Pro Ile Asp
405 410 415
ccg aag gac gtc ggc cgc gac tac gag gcc gtc atc cgc gtc aac tcc 1596
Pro Lys Asp Val Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser
420 425 430
cag tcc ggt aag ggc ggc gtc gcc tac atc atg aag acg gat cac ggc 1644
Gln Ser Gly Lys Gly Gly Val Ala Tyr Ile Met Lys Thr Asp His Gly
435 440 445
atc aac ctg ccg cgt cag atg cag gcc gag ttc tcc tcc gtc gtc cag 1692
Ile Asn Leu Pro Arg Gln Met Gln Ala Glu Phe Ser Ser Val Val Gln
450 455 460
gcg atc acc gac tcc gag ggc gga gag gtc aac tcc aag gag atg tgg 1740
Ala Ile Thr Asp Ser Glu Gly Gly Glu Val Asn Ser Lys Glu Met Trp
465 470 475 480
gac gtc ttc gcc acc gag tac ctg gat gtc acc gag ccg gtg gag gag 1788
Asp Val Phe Ala Thr Glu Tyr Leu Asp Val Thr Glu Pro Val Glu Glu
485 490 495
gtc tcc atc cgc atc gtc aac gcc gag acc gac acc gac gag gcc acc 1836
Val Ser Ile Arg Ile Val Asn Ala Glu Thr Asp Thr Asp Glu Ala Thr
500 505 510
gtc acc gcc acc gtc atc cac aag ggc tcc gag aag gag atc acc ggc 1884
Val Thr Ala Thr Val Ile His Lys Gly Ser Glu Lys Glu Ile Thr Gly
515 520 525
acc ggc aac ggc ccc gtc gcc gcc tac gcc aac gcc ctg gag cag ctg 1932
Thr Gly Asn Gly Pro Val Ala Ala Tyr Ala Asn Ala Leu Glu Gln Leu
530 535 540
ggc atc gac gcg gag gtc cag ttc tac tcc cag cag gcc cgc tcc tcg 1980
Gly Ile Asp Ala Glu Val Gln Phe Tyr Ser Gln Gln Ala Arg Ser Ser
545 550 555 560
ggc gat gac gcc gag gcc gcc tgc tac atc tac gcc acg gtc aac ggc 2028
Gly Asp Asp Ala Glu Ala Ala Cys Tyr Ile Tyr Ala Thr Val Asn Gly
565 570 575
tcc gcc gcc tgg ggc gtc ggt atc gcg ggc tcc atc acc cgc gcc tcc 2076
Ser Ala Ala Trp Gly Val Gly Ile Ala Gly Ser Ile Thr Arg Ala Ser
580 585 590
atc aag gca ctg acc tcc gcg gtc aac cgt gcc atc aag gtc gag gcc 2124
Ile Lys Ala Leu Thr Ser Ala Val Asn Arg Ala Ile Lys Val Glu Ala
595 600 605
tcc gtg ctg gcc ggc ggc gtc tag accgcctgag tcacgggagg ggagtgcgcg 2178
Ser Val Leu Ala Gly Gly Val
610 615
gccgcgcact cccctccttc cgtatctcac cgcccctacc ggcccctacc agcacagtcc 2238
ccgcaggaga ggcactagac tctgaacaat gaccacgacc ccagaccacg gcgattccgc 2298
agctgagacc ccgcctgccg tcgagccggc gttccccttc gtcgctctga cggtccaggc 2358
cacggggatc catccgtcga ccgcccgcct ggtggccgtc gacgtcctga ccttcgacga 2418
ggacggggag atcggcgagg agttccacac 2448
<210> 110
<211> 615
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 110
Met Ser Pro Asn Asp Ser Phe Ile Ser Ala Pro Ser Glu Ile Arg Thr
1 5 10 15
Pro Asp Gly Pro Arg Arg Glu Gly Gln Pro Phe Trp Asn Lys Gln Arg
20 25 30
Asn Ser Ser Met Pro Val His Arg Tyr Arg Pro Phe Ala Glu Ala Val
35 40 45
Glu Asp Ile Val Leu Pro Asp Arg Thr Trp Pro Asp Lys Lys Ile Thr
50 55 60
Ala Ala Pro Gln Trp Cys Ala Val Asp Leu Arg Asp Gly Asn Gln Ala
65 70 75 80
Leu Ile Asp Pro Met Ser Pro Glu Arg Lys Arg Arg Met Phe Asn Leu
85 90 95
Leu Val Gln Met Gly Tyr Lys Glu Ile Glu Val Gly Phe Pro Ser Ala
100 105 110
Ser Gln Thr Asp Phe Asp Phe Val Arg Glu Ile Ile Glu Gln Asp Met
115 120 125
Ile Pro Glu Asp Val Thr Ile Gln Val Leu Val Gln Ala Arg Glu His
130 135 140
Leu Ile Arg Arg Thr Phe Glu Ala Cys Glu Gly Ala Lys Asn Val Ile
145 150 155 160
Val His Phe Tyr Asn Ser Thr Ser Glu Leu Gln Arg Arg Val Val Phe
165 170 175
Arg Lys Asp Lys Pro Ala Ile Lys Gln Leu Ala Val Asp Ala Ala Glu
180 185 190
Leu Ile Gln Thr Ile Ala Gln Asp Tyr Pro Gly Thr Asn Trp Arg Trp
195 200 205
Gln Tyr Ser Pro Glu Ser Phe Thr Gly Thr Glu Leu Glu Phe Ala Leu
210 215 220
Glu Val Cys Asn Ala Val Ile Glu Val Met Ala Pro Thr Pro Asp Asn
225 230 235 240
Pro Ile Asn Ile Asn Leu Pro Ser Thr Val Glu Met Ile Thr Pro Asn
245 250 255
Val Tyr Ala Asp Ser Ile Glu Trp Met His Arg Asn Leu Asp Arg Arg
260 265 270
Asp Ser Val Ile Leu Ser Leu His Pro His Asn Asp Arg Gly Glu Gly
275 280 285
Val Ala Ala Ala Glu Leu Gly Tyr Leu Ala Gly Ala Asp Arg Ile Glu
290 295 300
Gly Cys Leu Phe Gly Asn Gly Glu Arg Thr Gly Asn Val Cys Leu Val
305 310 315 320
Thr Leu Gly Leu Asn Met Leu Thr Gln Gly Val Asp Pro Gln Ile Asp
325 330 335
Phe Ser Asp Ile Arg Gln Ile Arg Ser Thr Val Glu Tyr Cys Asn Gln
340 345 350
Leu Arg Val Pro Glu Arg His Pro Tyr Gly Gly Asp Leu Val Phe Thr
355 360 365
Ala Phe Ser Gly Ser His Gln Asp Ala Val Asn Lys Gly Leu Asp Ala
370 375 380
Met Ala Ala Lys Val Lys Pro Gly Ala Asn Asn Thr Glu Val Thr Trp
385 390 395 400
Glu Glu Leu Gly Lys Ala Val Trp Glu Val Pro Tyr Leu Pro Ile Asp
405 410 415
Pro Lys Asp Val Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser
420 425 430
Gln Ser Gly Lys Gly Gly Val Ala Tyr Ile Met Lys Thr Asp His Gly
435 440 445
Ile Asn Leu Pro Arg Gln Met Gln Ala Glu Phe Ser Ser Val Val Gln
450 455 460
Ala Ile Thr Asp Ser Glu Gly Gly Glu Val Asn Ser Lys Glu Met Trp
465 470 475 480
Asp Val Phe Ala Thr Glu Tyr Leu Asp Val Thr Glu Pro Val Glu Glu
485 490 495
Val Ser Ile Arg Ile Val Asn Ala Glu Thr Asp Thr Asp Glu Ala Thr
500 505 510
Val Thr Ala Thr Val Ile His Lys Gly Ser Glu Lys Glu Ile Thr Gly
515 520 525
Thr Gly Asn Gly Pro Val Ala Ala Tyr Ala Asn Ala Leu Glu Gln Leu
530 535 540
Gly Ile Asp Ala Glu Val Gln Phe Tyr Ser Gln Gln Ala Arg Ser Ser
545 550 555 560
Gly Asp Asp Ala Glu Ala Ala Cys Tyr Ile Tyr Ala Thr Val Asn Gly
565 570 575
Ser Ala Ala Trp Gly Val Gly Ile Ala Gly Ser Ile Thr Arg Ala Ser
580 585 590
Ile Lys Ala Leu Thr Ser Ala Val Asn Arg Ala Ile Lys Val Glu Ala
595 600 605
Ser Val Leu Ala Gly Gly Val
610 615
<210> 111
<211> 1614
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1314)
<223> ген leuB
<400> 111
ctggtcgtag tactcgtagt catagacgcg gacgtcgtcg ccgagcgcgg ccgccttctc 60
cggggaaccg gtgacggcgg cgacgagctc gacgtcgtcg agactgcgga gcgccgggag 120
gaaggcctgc tgggtgatct ggccgacacc gacgacagcg aaacggacag gggagtcata 180
tgcgtgtgtg ctcatggccc caggctacgg actcgaaacg tgcggcgttg agcgcacgag 240
gggaggggtg ggctgataga atcgccgcca gttcagcagg tgagaaagga atcccacaga 300
atg aaa ctt gca gtc att ggc gga gac ggc atc ggc ccg gag gtc acc 348
Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr
1 5 10 15
gaa gag gcg ctc aag gtc ctc cgc acc gtc cgc gac gac atc gag acc 396
Glu Glu Ala Leu Lys Val Leu Arg Thr Val Arg Asp Asp Ile Glu Thr
20 25 30
acc gac tac gat ctc ggc gca cgt cgc tac ctg cgc aac ggt gag ctg 444
Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Arg Asn Gly Glu Leu
35 40 45
ctc acc gac gcc gac ctc gac tcc ctc cgc gag cac gac gcc atc ctc 492
Leu Thr Asp Ala Asp Leu Asp Ser Leu Arg Glu His Asp Ala Ile Leu
50 55 60
ctc ggc gcc atc ggc gcc ccg gac acc gtc gcg ccg ggc gtc ctc gag 540
Leu Gly Ala Ile Gly Ala Pro Asp Thr Val Ala Pro Gly Val Leu Glu
65 70 75 80
cgt ggc ctg ctg ctc aag atg cgt ttc gcc ctc gac cac cac gtc aac 588
Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His Val Asn
85 90 95
ctg cgt ccc tcc aag ctc tac gag ggc gtc gag tcc ccg ctg aag aac 636
Leu Arg Pro Ser Lys Leu Tyr Glu Gly Val Glu Ser Pro Leu Lys Asn
100 105 110
ccg ggc gag atc gac ttc gtc gtc gtc cgc gag ggc acc gag ggc gcc 684
Pro Gly Glu Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu Gly Ala
115 120 125
tac acc ggc aac ggc ggc gcc atc cgc gtg ggc acc ccg cac gag gtc 732
Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His Glu Val
130 135 140
gcc aac gag acc tcc gtc aac acc cgc tac ggc gcc gag cgc gtc gtc 780
Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg Val Val
145 150 155 160
cgc tac gcc ttc gag ctg gcc atg acc cgc cgc aag cac gtc acc ctg 828
Arg Tyr Ala Phe Glu Leu Ala Met Thr Arg Arg Lys His Val Thr Leu
165 170 175
gtc cac aag acc aac gtg ctc gtc cac ggc ggc ggc atg tgg cag cgc 876
Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Met Trp Gln Arg
180 185 190
acc gtc gac gag gtc gcc acg gag tac cct gag gtc acc gtc gac tac 924
Thr Val Asp Glu Val Ala Thr Glu Tyr Pro Glu Val Thr Val Asp Tyr
195 200 205
gcg cac atc gac gcc gcg acg atc tac atg atc acc gac ccc tcc cgc 972
Ala His Ile Asp Ala Ala Thr Ile Tyr Met Ile Thr Asp Pro Ser Arg
210 215 220
ttc gac gtc atc gtc acc gac aac ctc ttc ggc gac atc atc acc gat 1020
Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Ile Thr Asp
225 230 235 240
gag gcc ggt gcc gtc tcc ggt ggc atc ggc ctc gcc gcc tcc ggc aac 1068
Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser Gly Asn
245 250 255
atc gac gcc acc cgc acc aac ccg tcc atg ttc gag ccg gtc cac ggc 1116
Ile Asp Ala Thr Arg Thr Asn Pro Ser Met Phe Glu Pro Val His Gly
260 265 270
tcc gca ccg gac atc gcc ggc cag ggc atc gcc gac ccg acc gcc gcc 1164
Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr Ala Ala
275 280 285
atc ctc tcc gcc gcg atg ctg ctg cgt cac ctc ggt gac gag gac aac 1212
Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu Asp Asn
290 295 300
gcc gca cgg atc gag gcc gcg gtg gcc gag gac gtc gcc aag cgt gag 1260
Ala Ala Arg Ile Glu Ala Ala Val Ala Glu Asp Val Ala Lys Arg Glu
305 310 315 320
ggg cag atc cgc acc acc gag gtc ggc gac cgc atc gcc gcc gcc ctc 1308
Gly Gln Ile Arg Thr Thr Glu Val Gly Asp Arg Ile Ala Ala Ala Leu
325 330 335
aag tag tctgtcggca gcagacagac gccatatata taaggagcat tcgtgaggcg 1364
Lys
acgtacagca ctactggccc tggccaccac cctggccatg gtcaccggat gctccacctc 1424
ggcggacacc ggtgacgggg acgaccggaa gcccggcacc cccgccctgg agatgacctc 1484
ctccgaggtc atcgccgacg ccgccggcaa cggcgtcgac acctccgccc gcctcttcga 1544
ggaggcggag accgtcgtgg tcgccgccga cacccctgag gaccaggccc ggggcgccca 1604
cctggcgatc 1614
<210> 112
<211> 337
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 112
Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr
1 5 10 15
Glu Glu Ala Leu Lys Val Leu Arg Thr Val Arg Asp Asp Ile Glu Thr
20 25 30
Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Arg Asn Gly Glu Leu
35 40 45
Leu Thr Asp Ala Asp Leu Asp Ser Leu Arg Glu His Asp Ala Ile Leu
50 55 60
Leu Gly Ala Ile Gly Ala Pro Asp Thr Val Ala Pro Gly Val Leu Glu
65 70 75 80
Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His Val Asn
85 90 95
Leu Arg Pro Ser Lys Leu Tyr Glu Gly Val Glu Ser Pro Leu Lys Asn
100 105 110
Pro Gly Glu Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu Gly Ala
115 120 125
Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His Glu Val
130 135 140
Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg Val Val
145 150 155 160
Arg Tyr Ala Phe Glu Leu Ala Met Thr Arg Arg Lys His Val Thr Leu
165 170 175
Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Met Trp Gln Arg
180 185 190
Thr Val Asp Glu Val Ala Thr Glu Tyr Pro Glu Val Thr Val Asp Tyr
195 200 205
Ala His Ile Asp Ala Ala Thr Ile Tyr Met Ile Thr Asp Pro Ser Arg
210 215 220
Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Ile Thr Asp
225 230 235 240
Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser Gly Asn
245 250 255
Ile Asp Ala Thr Arg Thr Asn Pro Ser Met Phe Glu Pro Val His Gly
260 265 270
Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr Ala Ala
275 280 285
Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu Asp Asn
290 295 300
Ala Ala Arg Ile Glu Ala Ala Val Ala Glu Asp Val Ala Lys Arg Glu
305 310 315 320
Gly Gln Ile Arg Thr Thr Glu Val Gly Asp Arg Ile Ala Ala Ala Leu
325 330 335
Lys
<210> 113
<211> 2019
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1719)
<223> ген leuC
<400> 113
gctggtcccg gccgccggtg ccgagggcgg tgagtgcggt gccggtggtc cagcggccgt 60
cgtcgccgcg ggcgaggatg cggtgtgtct cgagggcggt ggcgaggcgg tgggcggtgg 120
cgcggggcag gccggtggtc tcgcacagtt cggtgaggga gaggggatgg ggggcgacgg 180
ccatcatgat ggcgacggcc cgatcgagca ccttgatccc tgacactgcg ctatactgtc 240
ccataccacg atactaacat ctcaccttgt gagatttcac agatggaaag gtggacatcc 300
atg gcc gag aag ctc acg ctg gcc gag aag gtg tgg aac gac cat att 348
Met Ala Glu Lys Leu Thr Leu Ala Glu Lys Val Trp Asn Asp His Ile
1 5 10 15
gtc gcg aag ggc gag aac ggc gag ccc gat ctc ctc tac att gac ctg 396
Val Ala Lys Gly Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu
20 25 30
cag ctc ctg cac gag gtg acc tcc ccg cag gcc ttc gac gga ctg cgc 444
Gln Leu Leu His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg
35 40 45
ctc gcc ggc cgc aag ctc cgc cgc ccg gac ctg cac ctg gcc acc gag 492
Leu Ala Gly Arg Lys Leu Arg Arg Pro Asp Leu His Leu Ala Thr Glu
50 55 60
gac cac aac gtg ccc acc gag ggc atc gtc tcc ggc tcc ctc ctg gag 540
Asp His Asn Val Pro Thr Glu Gly Ile Val Ser Gly Ser Leu Leu Glu
65 70 75 80
atc aag gag gcc acc tcc cgc acg cag gtc gag acc ctg cgc aag aac 588
Ile Lys Glu Ala Thr Ser Arg Thr Gln Val Glu Thr Leu Arg Lys Asn
85 90 95
tgc gag gag ttc ggc atc cgc ctg cac ccg atg ggc gac gtc aag cag 636
Cys Glu Glu Phe Gly Ile Arg Leu His Pro Met Gly Asp Val Lys Gln
100 105 110
ggc atc gtc cac acc gtc ggc ccg cag ctg ggc gcc acc cag ccg ggc 684
Gly Ile Val His Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly
115 120 125
atg acc atc gtc tgc ggc gac tcc cac acc tcc acc cac ggt gcc ttc 732
Met Thr Ile Val Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe
130 135 140
ggc tcc atc gcc atg ggc atc ggc acc tcc gag gtc gag cac gtc atg 780
Gly Ser Ile Ala Met Gly Ile Gly Thr Ser Glu Val Glu His Val Met
145 150 155 160
gcg acc cag acc ctg ccg ctc aag ccg ttc aag acc atg gcc atc gag 828
Ala Thr Gln Thr Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu
165 170 175
gtc tcc ggc gag ctg cag gag ggc gtc acc gcc aag gac ctc atc ttg 876
Val Ser Gly Glu Leu Gln Glu Gly Val Thr Ala Lys Asp Leu Ile Leu
180 185 190
gcg atc atc gcc aag atc ggc acc ggc ggc ggc cag ggc cac atc atc 924
Ala Ile Ile Ala Lys Ile Gly Thr Gly Gly Gly Gln Gly His Ile Ile
195 200 205
gag tac cgc ggc gag gcc atc gag aag ctc tcg atg gag gcc cgg atg 972
Glu Tyr Arg Gly Glu Ala Ile Glu Lys Leu Ser Met Glu Ala Arg Met
210 215 220
acc atc tgc aac atg tcc atc gag gcc ggt gcc cgt gcc ggc atg gtc 1020
Thr Ile Cys Asn Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Val
225 230 235 240
gcc ccc gac cag aag acc ttc gac tac gtc cag ggc cgc gag ttc gcc 1068
Ala Pro Asp Gln Lys Thr Phe Asp Tyr Val Gln Gly Arg Glu Phe Ala
245 250 255
ccc acc ggc gag gac tgg gac gcc gcc gtc gcc tac tgg aag acc ctg 1116
Pro Thr Gly Glu Asp Trp Asp Ala Ala Val Ala Tyr Trp Lys Thr Leu
260 265 270
ccg acc gac gag ggc gcc gag ttc gac acc gtc gtc gag atc gac ggc 1164
Pro Thr Asp Glu Gly Ala Glu Phe Asp Thr Val Val Glu Ile Asp Gly
275 280 285
tcc gcg ctg acc ccg ttc gtc acc tgg ggc acc aac ccg ggc cag ggc 1212
Ser Ala Leu Thr Pro Phe Val Thr Trp Gly Thr Asn Pro Gly Gln Gly
290 295 300
ctg ccc ctg tcc gcg aac gtc ccg gac ccg gag gac ttc acc aac gag 1260
Leu Pro Leu Ser Ala Asn Val Pro Asp Pro Glu Asp Phe Thr Asn Glu
305 310 315 320
gcc gac aag gcc gcc gcg gag aag gcc ctg gcc tac atg gac ctc acc 1308
Ala Asp Lys Ala Ala Ala Glu Lys Ala Leu Ala Tyr Met Asp Leu Thr
325 330 335
ccg ggc acc ccg ctg cgg gac atc aag atc gac acc gtc ttc ctc ggc 1356
Pro Gly Thr Pro Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly
340 345 350
tcc tgc acc aac gcc cgc atc gag gac ctg cgc gct gcc gcc gag gtc 1404
Ser Cys Thr Asn Ala Arg Ile Glu Asp Leu Arg Ala Ala Ala Glu Val
355 360 365
gtc gag ggc cgc acg atc gcc ccg aac acc cgc atg ctc gtc gtc ccg 1452
Val Glu Gly Arg Thr Ile Ala Pro Asn Thr Arg Met Leu Val Val Pro
370 375 380
tcc tcg acg ctg gtc aag cag cag gcc gag gag gag ggg ctg gac aag 1500
Ser Ser Thr Leu Val Lys Gln Gln Ala Glu Glu Glu Gly Leu Asp Lys
385 390 395 400
atc ttc acc gac ttc ggc gcc gag tgg cgc acc gcc ggc tgc tcc atg 1548
Ile Phe Thr Asp Phe Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met
405 410 415
tgc ctg ggc atg aac ccg gac cag ctg gcc ccg ggc gag cgc tcc gcc 1596
Cys Leu Gly Met Asn Pro Asp Gln Leu Ala Pro Gly Glu Arg Ser Ala
420 425 430
tcc acc tcg aac cgt aac ttc gag ggc cgg cag ggc ccg ggc ggc cgc 1644
Ser Thr Ser Asn Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg
435 440 445
acc cac ctg gtg tcc ccg ccg gtc gcc gcc gcc acc gcc gtc ctc ggc 1692
Thr His Leu Val Ser Pro Pro Val Ala Ala Ala Thr Ala Val Leu Gly
450 455 460
cac ctg gct tcc ccc gca gac ctc tga ccacgccgac caccaaggag 1739
His Leu Ala Ser Pro Ala Asp Leu
465 470
ataaaaacag tggagaagtt caccacccac accggcgtcg gcgtcccgct gacccgctcg 1799
aacgtcgaca ccgaccagat catccccgcc gtctacctca agcgcgtcac ccgcacgggc 1859
ttcgatgacg gcctgttcgc cgagtggcgc aaggacgatt ccttcgtcct caaccaggag 1919
ccctaccgca acggctccgt cctggtcgcc ggccccgact tcggcaccgg ctcctcccgt 1979
gagcacgccg tctgggccct catggactac ggcttccgcg 2019
<210> 114
<211> 472
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 114
Met Ala Glu Lys Leu Thr Leu Ala Glu Lys Val Trp Asn Asp His Ile
1 5 10 15
Val Ala Lys Gly Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu
20 25 30
Gln Leu Leu His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg
35 40 45
Leu Ala Gly Arg Lys Leu Arg Arg Pro Asp Leu His Leu Ala Thr Glu
50 55 60
Asp His Asn Val Pro Thr Glu Gly Ile Val Ser Gly Ser Leu Leu Glu
65 70 75 80
Ile Lys Glu Ala Thr Ser Arg Thr Gln Val Glu Thr Leu Arg Lys Asn
85 90 95
Cys Glu Glu Phe Gly Ile Arg Leu His Pro Met Gly Asp Val Lys Gln
100 105 110
Gly Ile Val His Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly
115 120 125
Met Thr Ile Val Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe
130 135 140
Gly Ser Ile Ala Met Gly Ile Gly Thr Ser Glu Val Glu His Val Met
145 150 155 160
Ala Thr Gln Thr Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu
165 170 175
Val Ser Gly Glu Leu Gln Glu Gly Val Thr Ala Lys Asp Leu Ile Leu
180 185 190
Ala Ile Ile Ala Lys Ile Gly Thr Gly Gly Gly Gln Gly His Ile Ile
195 200 205
Glu Tyr Arg Gly Glu Ala Ile Glu Lys Leu Ser Met Glu Ala Arg Met
210 215 220
Thr Ile Cys Asn Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Val
225 230 235 240
Ala Pro Asp Gln Lys Thr Phe Asp Tyr Val Gln Gly Arg Glu Phe Ala
245 250 255
Pro Thr Gly Glu Asp Trp Asp Ala Ala Val Ala Tyr Trp Lys Thr Leu
260 265 270
Pro Thr Asp Glu Gly Ala Glu Phe Asp Thr Val Val Glu Ile Asp Gly
275 280 285
Ser Ala Leu Thr Pro Phe Val Thr Trp Gly Thr Asn Pro Gly Gln Gly
290 295 300
Leu Pro Leu Ser Ala Asn Val Pro Asp Pro Glu Asp Phe Thr Asn Glu
305 310 315 320
Ala Asp Lys Ala Ala Ala Glu Lys Ala Leu Ala Tyr Met Asp Leu Thr
325 330 335
Pro Gly Thr Pro Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly
340 345 350
Ser Cys Thr Asn Ala Arg Ile Glu Asp Leu Arg Ala Ala Ala Glu Val
355 360 365
Val Glu Gly Arg Thr Ile Ala Pro Asn Thr Arg Met Leu Val Val Pro
370 375 380
Ser Ser Thr Leu Val Lys Gln Gln Ala Glu Glu Glu Gly Leu Asp Lys
385 390 395 400
Ile Phe Thr Asp Phe Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met
405 410 415
Cys Leu Gly Met Asn Pro Asp Gln Leu Ala Pro Gly Glu Arg Ser Ala
420 425 430
Ser Thr Ser Asn Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg
435 440 445
Thr His Leu Val Ser Pro Pro Val Ala Ala Ala Thr Ala Val Leu Gly
450 455 460
His Leu Ala Ser Pro Ala Asp Leu
465 470
<210> 115
<211> 1197
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(897)
<223> ген leuD
<400> 115
cccgtcctcg acgctggtca agcagcaggc cgaggaggag gggctggaca agatcttcac 60
cgacttcggc gccgagtggc gcaccgccgg ctgctccatg tgcctgggca tgaacccgga 120
ccagctggcc ccgggcgagc gctccgcctc cacctcgaac cgtaacttcg agggccggca 180
gggcccgggc ggccgcaccc acctggtgtc cccgccggtc gccgccgcca ccgccgtcct 240
cggccacctg gcttcccccg cagacctctg accacgccga ccaccaagga gataaaaaca 300
gtg gag aag ttc acc acc cac acc ggc gtc ggc gtc ccg ctg acc cgc 348
Val Glu Lys Phe Thr Thr His Thr Gly Val Gly Val Pro Leu Thr Arg
1 5 10 15
tcg aac gtc gac acc gac cag atc atc ccc gcc gtc tac ctc aag cgc 396
Ser Asn Val Asp Thr Asp Gln Ile Ile Pro Ala Val Tyr Leu Lys Arg
20 25 30
gtc acc cgc acg ggc ttc gat gac ggc ctg ttc gcc gag tgg cgc aag 444
Val Thr Arg Thr Gly Phe Asp Asp Gly Leu Phe Ala Glu Trp Arg Lys
35 40 45
gac gat tcc ttc gtc ctc aac cag gag ccc tac cgc aac ggc tcc gtc 492
Asp Asp Ser Phe Val Leu Asn Gln Glu Pro Tyr Arg Asn Gly Ser Val
50 55 60
ctg gtc gcc ggc ccc gac ttc ggc acc ggc tcc tcc cgt gag cac gcc 540
Leu Val Ala Gly Pro Asp Phe Gly Thr Gly Ser Ser Arg Glu His Ala
65 70 75 80
gtc tgg gcc ctc atg gac tac ggc ttc cgc gtc gtg ctg tcc tcc cgc 588
Val Trp Ala Leu Met Asp Tyr Gly Phe Arg Val Val Leu Ser Ser Arg
85 90 95
ttc gcc gac atc ttc cgc ggc aac tcc ggc aag gcc ggc ctg ctg gcc 636
Phe Ala Asp Ile Phe Arg Gly Asn Ser Gly Lys Ala Gly Leu Leu Ala
100 105 110
gcc cag atg gag gag gcc gat atc gag ctg ctg tgg aag cag ctc gag 684
Ala Gln Met Glu Glu Ala Asp Ile Glu Leu Leu Trp Lys Gln Leu Glu
115 120 125
gct gag ccg ggc ctg gag atc acc gtc gac ctc gag tcc cgc acc gtc 732
Ala Glu Pro Gly Leu Glu Ile Thr Val Asp Leu Glu Ser Arg Thr Val
130 135 140
acc gcc ggc gag aac acc tac cag ttc tcc gtc gac gac tac acc cgc 780
Thr Ala Gly Glu Asn Thr Tyr Gln Phe Ser Val Asp Asp Tyr Thr Arg
145 150 155 160
tgg cgc ctc atg gag ggc ctc gac gac atc ggc ctg acc ctg cgc gac 828
Trp Arg Leu Met Glu Gly Leu Asp Asp Ile Gly Leu Thr Leu Arg Asp
165 170 175
gag cag gcc atc acc gac tac gag gcg aag cgc ccc gtg tac aag ccg 876
Glu Gln Ala Ile Thr Asp Tyr Glu Ala Lys Arg Pro Val Tyr Lys Pro
180 185 190
acc atc acc gcg gag gtg tga gtcatgtccg acaaccgttt cgacgaggga 927
Thr Ile Thr Ala Glu Val
195
atccgcatcc gccgcgaggt catgggcgac gatttcgtcg acgccgccct gacccgcaac 987
gccggcaccg acggcgagga cctgcagcac cacatcaccg ccaccgtctg gggttcggtg 1047
tggacccgcg agggcctgtc caagcgtgac cgcagcctgc tcaacatcgg catgctcgtc 1107
gccctgcgcg cgaccgagga actgcgcgga catgtccgcg gcggcctgcg taacggcctg 1167
acccgcgagg agatcaccga ggcgatcatc 1197
<210> 116
<211> 198
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 116
Val Glu Lys Phe Thr Thr His Thr Gly Val Gly Val Pro Leu Thr Arg
1 5 10 15
Ser Asn Val Asp Thr Asp Gln Ile Ile Pro Ala Val Tyr Leu Lys Arg
20 25 30
Val Thr Arg Thr Gly Phe Asp Asp Gly Leu Phe Ala Glu Trp Arg Lys
35 40 45
Asp Asp Ser Phe Val Leu Asn Gln Glu Pro Tyr Arg Asn Gly Ser Val
50 55 60
Leu Val Ala Gly Pro Asp Phe Gly Thr Gly Ser Ser Arg Glu His Ala
65 70 75 80
Val Trp Ala Leu Met Asp Tyr Gly Phe Arg Val Val Leu Ser Ser Arg
85 90 95
Phe Ala Asp Ile Phe Arg Gly Asn Ser Gly Lys Ala Gly Leu Leu Ala
100 105 110
Ala Gln Met Glu Glu Ala Asp Ile Glu Leu Leu Trp Lys Gln Leu Glu
115 120 125
Ala Glu Pro Gly Leu Glu Ile Thr Val Asp Leu Glu Ser Arg Thr Val
130 135 140
Thr Ala Gly Glu Asn Thr Tyr Gln Phe Ser Val Asp Asp Tyr Thr Arg
145 150 155 160
Trp Arg Leu Met Glu Gly Leu Asp Asp Ile Gly Leu Thr Leu Arg Asp
165 170 175
Glu Gln Ala Ile Thr Asp Tyr Glu Ala Lys Arg Pro Val Tyr Lys Pro
180 185 190
Thr Ile Thr Ala Glu Val
195
<210> 117
<211> 1398
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1098)
<223> ген panB
<400> 117
ccgggcgcaa ggcggacgta ctcgtcattc cgccaacccc ggatgattct ctgacgctct 60
gccccggtgg cacgcgtggt gctgatcacc ggccccccga cctggttcat gtccgcagtc 120
tcccatcagc gcctgcggca cgcacctcca cacgtcggtg gcgttgtgag gcacccgctt 180
gttcgcgagc tccgggagtt ttcccaggtc aggttttcct ggggtgaaca agagggtact 240
tccgcacaca tgccttccgt gggaacaggg gggcatatac tcttgcccat ggtttcccgg 300
atg cgt acc cgc cat ttc gcc cag gcc aag gcc gag ggc cgc aag atc 348
Met Arg Thr Arg His Phe Ala Gln Ala Lys Ala Glu Gly Arg Lys Ile
1 5 10 15
tcc tgc ctg acc gcc tac gac gcg ccc acc gcc gcg atc ttc gat gag 396
Ser Cys Leu Thr Ala Tyr Asp Ala Pro Thr Ala Ala Ile Phe Asp Glu
20 25 30
gcg ggc atc gac ctg ctc ctc gtc ggc gat tcc gcc gcc aac gtc gtc 444
Ala Gly Ile Asp Leu Leu Leu Val Gly Asp Ser Ala Ala Asn Val Val
35 40 45
ctg ggc cag gac tcg acc ctg tcg atc acg gtc gag gag atg atg acc 492
Leu Gly Gln Asp Ser Thr Leu Ser Ile Thr Val Glu Glu Met Met Thr
50 55 60
atg gcg acc gcc gtc gcc cgc ggc acc gaa cgc gcc ttc gtg gtc acc 540
Met Ala Thr Ala Val Ala Arg Gly Thr Glu Arg Ala Phe Val Val Thr
65 70 75 80
gac ctg ccc ttc ggc tcc tac gag gtc tcc ccc gcc cag gcg ctg gag 588
Asp Leu Pro Phe Gly Ser Tyr Glu Val Ser Pro Ala Gln Ala Leu Glu
85 90 95
acg gcc gcc cgg ttc atg aag cag acc ggt gtc gcc gcg gtg aag atc 636
Thr Ala Ala Arg Phe Met Lys Gln Thr Gly Val Ala Ala Val Lys Ile
100 105 110
gag ggc ggc gtc gag atc gcc ccg acg atc cgc acg ctc gtc gac gcc 684
Glu Gly Gly Val Glu Ile Ala Pro Thr Ile Arg Thr Leu Val Asp Ala
115 120 125
ggc atc ccc gtc atg gcc cac atc ggc tac acc ccg cag tcc gag cac 732
Gly Ile Pro Val Met Ala His Ile Gly Tyr Thr Pro Gln Ser Glu His
130 135 140
gcc ctc ggc ggc tac gtc gtc cag ggt cgc ggg gag gcc gcg gag aag 780
Ala Leu Gly Gly Tyr Val Val Gln Gly Arg Gly Glu Ala Ala Glu Lys
145 150 155 160
ctg cgc gcc gac gcc ctc gcc gtc cag gag gcc ggc gcc ttc gcc gtc 828
Leu Arg Ala Asp Ala Leu Ala Val Gln Glu Ala Gly Ala Phe Ala Val
165 170 175
gtc ctc gag atg gtc ccc gcc ggc atc gcc ggg gag atc acc cgc gag 876
Val Leu Glu Met Val Pro Ala Gly Ile Ala Gly Glu Ile Thr Arg Glu
180 185 190
ctc acc atc ccg acg atc ggc atc ggc gcc ggc gcc gac acc gac ggc 924
Leu Thr Ile Pro Thr Ile Gly Ile Gly Ala Gly Ala Asp Thr Asp Gly
195 200 205
cag gtc ctc gtg tgg acc gac gcc ttc ggt ctc acg cgc ggc cgt acg 972
Gln Val Leu Val Trp Thr Asp Ala Phe Gly Leu Thr Arg Gly Arg Thr
210 215 220
ccg cgt ttc gcg cgt cgt tac gcg gag atc ggg gac atg ctc ctc gac 1020
Pro Arg Phe Ala Arg Arg Tyr Ala Glu Ile Gly Asp Met Leu Leu Asp
225 230 235 240
gcc gcc cgt gcc tac cgc acc gac gtc gag tcc ggc tcc ttc ccg gac 1068
Ala Ala Arg Ala Tyr Arg Thr Asp Val Glu Ser Gly Ser Phe Pro Asp
245 250 255
gag tcc gag tcc ttc cgg gac gag cag tga ccgtcgtcgc gcgcactgtc 1118
Glu Ser Glu Ser Phe Arg Asp Glu Gln
260 265
gcgcagctgc gttccgccgt cgccgatctg cagggttccg tcggcctcgt gccgaccatg 1178
ggtgcgctcc acgagggtca cggccgtctg gtgtccctgg ccgccgagga gaactcctcg 1238
gtggtggtga gcatcttcgt caacccgttg cagttcaccg acctcggcga ctgcgacgac 1298
ttccgccact acccgcgtga cctggacaag gacgtcgcct tcctggagaa gctcggcgtc 1358
gacgtcgtgt tcgcgccgac cgacgaggcg atgtacccgg 1398
<210> 118
<211> 265
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 118
Met Arg Thr Arg His Phe Ala Gln Ala Lys Ala Glu Gly Arg Lys Ile
1 5 10 15
Ser Cys Leu Thr Ala Tyr Asp Ala Pro Thr Ala Ala Ile Phe Asp Glu
20 25 30
Ala Gly Ile Asp Leu Leu Leu Val Gly Asp Ser Ala Ala Asn Val Val
35 40 45
Leu Gly Gln Asp Ser Thr Leu Ser Ile Thr Val Glu Glu Met Met Thr
50 55 60
Met Ala Thr Ala Val Ala Arg Gly Thr Glu Arg Ala Phe Val Val Thr
65 70 75 80
Asp Leu Pro Phe Gly Ser Tyr Glu Val Ser Pro Ala Gln Ala Leu Glu
85 90 95
Thr Ala Ala Arg Phe Met Lys Gln Thr Gly Val Ala Ala Val Lys Ile
100 105 110
Glu Gly Gly Val Glu Ile Ala Pro Thr Ile Arg Thr Leu Val Asp Ala
115 120 125
Gly Ile Pro Val Met Ala His Ile Gly Tyr Thr Pro Gln Ser Glu His
130 135 140
Ala Leu Gly Gly Tyr Val Val Gln Gly Arg Gly Glu Ala Ala Glu Lys
145 150 155 160
Leu Arg Ala Asp Ala Leu Ala Val Gln Glu Ala Gly Ala Phe Ala Val
165 170 175
Val Leu Glu Met Val Pro Ala Gly Ile Ala Gly Glu Ile Thr Arg Glu
180 185 190
Leu Thr Ile Pro Thr Ile Gly Ile Gly Ala Gly Ala Asp Thr Asp Gly
195 200 205
Gln Val Leu Val Trp Thr Asp Ala Phe Gly Leu Thr Arg Gly Arg Thr
210 215 220
Pro Arg Phe Ala Arg Arg Tyr Ala Glu Ile Gly Asp Met Leu Leu Asp
225 230 235 240
Ala Ala Arg Ala Tyr Arg Thr Asp Val Glu Ser Gly Ser Phe Pro Asp
245 250 255
Glu Ser Glu Ser Phe Arg Asp Glu Gln
260 265
<210> 119
<211> 1452
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1152)
<223> ген panC
<400> 119
cctcgccgtc caggaggccg gcgccttcgc cgtcgtcctc gagatggtcc ccgccggcat 60
cgccggggag atcacccgcg agctcaccat cccgacgatc ggcatcggcg ccggcgccga 120
caccgacggc caggtcctcg tgtggaccga cgccttcggt ctcacgcgcg gccgtacgcc 180
gcgtttcgcg cgtcgttacg cggagatcgg ggacatgctc ctcgacgccg cccgtgccta 240
ccgcaccgac gtcgagtccg gctccttccc ggacgagtcc gagtccttcc gggacgagca 300
gtg acc gtc gtc gcg cgc act gtc gcg cag ctg cgt tcc gcc gtc gcc 348
Val Thr Val Val Ala Arg Thr Val Ala Gln Leu Arg Ser Ala Val Ala
1 5 10 15
gat ctg cag ggt tcc gtc ggc ctc gtg ccg acc atg ggt gcg ctc cac 396
Asp Leu Gln Gly Ser Val Gly Leu Val Pro Thr Met Gly Ala Leu His
20 25 30
gag ggt cac ggc cgt ctg gtg tcc ctg gcc gcc gag gag aac tcc tcg 444
Glu Gly His Gly Arg Leu Val Ser Leu Ala Ala Glu Glu Asn Ser Ser
35 40 45
gtg gtg gtg agc atc ttc gtc aac ccg ttg cag ttc acc gac ctc ggc 492
Val Val Val Ser Ile Phe Val Asn Pro Leu Gln Phe Thr Asp Leu Gly
50 55 60
gac tgc gac gac ttc cgc cac tac ccg cgt gac ctg gac aag gac gtc 540
Asp Cys Asp Asp Phe Arg His Tyr Pro Arg Asp Leu Asp Lys Asp Val
65 70 75 80
gcc ttc ctg gag aag ctc ggc gtc gac gtc gtg ttc gcg ccg acc gac 588
Ala Phe Leu Glu Lys Leu Gly Val Asp Val Val Phe Ala Pro Thr Asp
85 90 95
gag gcg atg tac ccg gac ggc ctg ccc cag gtg tgg gtg cgg gcc ggc 636
Glu Ala Met Tyr Pro Asp Gly Leu Pro Gln Val Trp Val Arg Ala Gly
100 105 110
gag atg ggt gag cgc ctc gag ggc gcc tcc cgt ccc ggc cac ttc gac 684
Glu Met Gly Glu Arg Leu Glu Gly Ala Ser Arg Pro Gly His Phe Asp
115 120 125
ggc gtg acc acc gtg gtg gcc aag ctg ttc cag ctc atc cag ccg gac 732
Gly Val Thr Thr Val Val Ala Lys Leu Phe Gln Leu Ile Gln Pro Asp
130 135 140
cgc gcc tac ttc ggt gag aag gac gcg cag cag ctg gcg atc atc cag 780
Arg Ala Tyr Phe Gly Glu Lys Asp Ala Gln Gln Leu Ala Ile Ile Gln
145 150 155 160
cgc atg gtc atg gac ctg gac ttc ccg ctg cag atc cgc ccg gtg ccg 828
Arg Met Val Met Asp Leu Asp Phe Pro Leu Gln Ile Arg Pro Val Pro
165 170 175
atc atc cgc ggt gcc gac ggc ctg gcc gag tcc tcc cgc aac cag cac 876
Ile Ile Arg Gly Ala Asp Gly Leu Ala Glu Ser Ser Arg Asn Gln His
180 185 190
ctc acc ccc gag gcg cgg gag cag gcg ctc gtc ctg ccg cgc acc ctg 924
Leu Thr Pro Glu Ala Arg Glu Gln Ala Leu Val Leu Pro Arg Thr Leu
195 200 205
gcc gcg ctg cgt gac cgc gcc gcc gcc ggt gac ccc ctc gac gtc gag 972
Ala Ala Leu Arg Asp Arg Ala Ala Ala Gly Asp Pro Leu Asp Val Glu
210 215 220
ggt gcc cgc gag gcg ctg cgc tcc gcc gag ggc gtg gaa ctc gac cac 1020
Gly Ala Arg Glu Ala Leu Arg Ser Ala Glu Gly Val Glu Leu Asp His
225 230 235 240
ctc gag gtc gtc gac gcc gcg acg ctg ctg ccg gtc tcc gag gtc ggc 1068
Leu Glu Val Val Asp Ala Ala Thr Leu Leu Pro Val Ser Glu Val Gly
245 250 255
gcc ggc gcg ctg gcc gtc gcc gcg atc cac gtg ggt ggc acg cgg ctc 1116
Ala Gly Ala Leu Ala Val Ala Ala Ile His Val Gly Gly Thr Arg Leu
260 265 270
atc gac aac gtg cga ctg ctt ccg ccg cgc gct tag cgtaggggcc 1162
Ile Asp Asn Val Arg Leu Leu Pro Pro Arg Ala
275 280
gacgccgacg atcgtcgccg aaccggggcc gagccagtcc ccgtagccca ccaggtgcag 1222
cggcagcccc cggtcgagga cccgccggaa gggacgcagc gccgggcgga agccggtgca 1282
ccagatgagg tgctccgcgt ccacctcgtc gaggctgcgg aacagcggcg tcgcccgcag 1342
gcggccggag tcccgcgcgg ccttcaccgc gggcaggacc acgatgtccc cgagctcgga 1402
gtccgccccc gggtcctcct ccccgcgcag gatcgccagg gcccgcaggc 1452
<210> 120
<211> 283
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 120
Val Thr Val Val Ala Arg Thr Val Ala Gln Leu Arg Ser Ala Val Ala
1 5 10 15
Asp Leu Gln Gly Ser Val Gly Leu Val Pro Thr Met Gly Ala Leu His
20 25 30
Glu Gly His Gly Arg Leu Val Ser Leu Ala Ala Glu Glu Asn Ser Ser
35 40 45
Val Val Val Ser Ile Phe Val Asn Pro Leu Gln Phe Thr Asp Leu Gly
50 55 60
Asp Cys Asp Asp Phe Arg His Tyr Pro Arg Asp Leu Asp Lys Asp Val
65 70 75 80
Ala Phe Leu Glu Lys Leu Gly Val Asp Val Val Phe Ala Pro Thr Asp
85 90 95
Glu Ala Met Tyr Pro Asp Gly Leu Pro Gln Val Trp Val Arg Ala Gly
100 105 110
Glu Met Gly Glu Arg Leu Glu Gly Ala Ser Arg Pro Gly His Phe Asp
115 120 125
Gly Val Thr Thr Val Val Ala Lys Leu Phe Gln Leu Ile Gln Pro Asp
130 135 140
Arg Ala Tyr Phe Gly Glu Lys Asp Ala Gln Gln Leu Ala Ile Ile Gln
145 150 155 160
Arg Met Val Met Asp Leu Asp Phe Pro Leu Gln Ile Arg Pro Val Pro
165 170 175
Ile Ile Arg Gly Ala Asp Gly Leu Ala Glu Ser Ser Arg Asn Gln His
180 185 190
Leu Thr Pro Glu Ala Arg Glu Gln Ala Leu Val Leu Pro Arg Thr Leu
195 200 205
Ala Ala Leu Arg Asp Arg Ala Ala Ala Gly Asp Pro Leu Asp Val Glu
210 215 220
Gly Ala Arg Glu Ala Leu Arg Ser Ala Glu Gly Val Glu Leu Asp His
225 230 235 240
Leu Glu Val Val Asp Ala Ala Thr Leu Leu Pro Val Ser Glu Val Gly
245 250 255
Ala Gly Ala Leu Ala Val Ala Ala Ile His Val Gly Gly Thr Arg Leu
260 265 270
Ile Asp Asn Val Arg Leu Leu Pro Pro Arg Ala
275 280
<210> 121
<211> 1116
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(816)
<223> ген ilvH
<400> 121
gcgtcacccg cgaggaggac gtcctgccga cgatccagaa ggcacgcgag atcaacgacc 60
gcccggtcgt catcgacttc atcgtcggtg aggacgccca ggtctggccg atggtcgccg 120
ccggcgcctc caactcggac atcgagtacg cacgtggtct gcgccccttc ttcgagggtg 180
acgagtccgc gggtgagtcc ccggccgaca tccacggtgt catggagaac gcccaggagg 240
agaccgtcga ggaagtcctc gaggagcgcg cgaacgacag cgagaaggag aactaggaca 300
atg gcg aac acc gac atc atc cgc aac acc ctc agc gtc ctg gtc cag 348
Met Ala Asn Thr Asp Ile Ile Arg Asn Thr Leu Ser Val Leu Val Gln
1 5 10 15
gac gtc gac ggc atc atc tcc cgc gtc gca ggc atg ttc acc cgc cgt 396
Asp Val Asp Gly Ile Ile Ser Arg Val Ala Gly Met Phe Thr Arg Arg
20 25 30
ggc tac aac ctg gtg tcc atc gtc tcg gcg cac acc gac acc ccg ggc 444
Gly Tyr Asn Leu Val Ser Ile Val Ser Ala His Thr Asp Thr Pro Gly
35 40 45
atc agc cgc atc acc atc gtg gtc gac gcc aac gag ctg gtc atc gag 492
Ile Ser Arg Ile Thr Ile Val Val Asp Ala Asn Glu Leu Val Ile Glu
50 55 60
cag gtc acc aag cag ctc aac aag atc atc ccg gtg ctc aag gtc gtc 540
Gln Val Thr Lys Gln Leu Asn Lys Ile Ile Pro Val Leu Lys Val Val
65 70 75 80
cgc ctc gag gag gac acc acc atc gcc cgc gcc atc atg ctg gtg aag 588
Arg Leu Glu Glu Asp Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys
85 90 95
gtc aac gcc gac aac acc aac cgc ccg cag gtc gtc gac gcc gcg aac 636
Val Asn Ala Asp Asn Thr Asn Arg Pro Gln Val Val Asp Ala Ala Asn
100 105 110
atc ttc cgc gcc cgc atc gtc gac gtg gcc cag gag tcc gtg gtc atc 684
Ile Phe Arg Ala Arg Ile Val Asp Val Ala Gln Glu Ser Val Val Ile
115 120 125
gag gcc acc ggt acc ccg ggc aag ctg cgc gcc ctg ctg gag gtc ctc 732
Glu Ala Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Glu Val Leu
130 135 140
gag ccc ttc ggt atc cgt gag ctc atc cag tcc ggt cag atc gcc ctc 780
Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu
145 150 155 160
aac cgc ggt ccg aag acg atg gca ccg tcc aag taa caccccctca 826
Asn Arg Gly Pro Lys Thr Met Ala Pro Ser Lys
165 170
tcccacctgg tgagacaaca cgcatgaaag tcccaccagg tggggtaccc ctgtctcatg 886
ttgtggtaca aacaacaaca gcacttgtat aagacgaaag gcgagttttt ctcccatggc 946
tattgaactg ctttacgacg ccgacgctga cctctccctc atccagggcc gcaaggtcgc 1006
catcatcggc tacggctccc agggccacgc ccacgcccag aacctgcgtg actccggcgt 1066
cgaggtctgc atcggcctgc gcgacggctc caagtccgcg gagaaggcca 1116
<210> 122
<211> 171
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 122
Met Ala Asn Thr Asp Ile Ile Arg Asn Thr Leu Ser Val Leu Val Gln
1 5 10 15
Asp Val Asp Gly Ile Ile Ser Arg Val Ala Gly Met Phe Thr Arg Arg
20 25 30
Gly Tyr Asn Leu Val Ser Ile Val Ser Ala His Thr Asp Thr Pro Gly
35 40 45
Ile Ser Arg Ile Thr Ile Val Val Asp Ala Asn Glu Leu Val Ile Glu
50 55 60
Gln Val Thr Lys Gln Leu Asn Lys Ile Ile Pro Val Leu Lys Val Val
65 70 75 80
Arg Leu Glu Glu Asp Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys
85 90 95
Val Asn Ala Asp Asn Thr Asn Arg Pro Gln Val Val Asp Ala Ala Asn
100 105 110
Ile Phe Arg Ala Arg Ile Val Asp Val Ala Gln Glu Ser Val Val Ile
115 120 125
Glu Ala Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Glu Val Leu
130 135 140
Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu
145 150 155 160
Asn Arg Gly Pro Lys Thr Met Ala Pro Ser Lys
165 170
<210> 123
<211> 1944
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1644)
<223> ген gdh
<400> 123
gaaggtggcg gcggtgccct cacccccgtc ggccatggga gcgagggtga tctcggcgtc 60
ccggatgatc gagcggacac cctcgccgat cagccgtgcg gcctgatcgg cggtggcagt 120
gcccttgaag gagtcggggg cgatgatgat gtggaccatg ggaagcagtc taggggtagt 180
gggagtgaca tattcaactc cggagtgact tgcatgaacc gcgccgcccg tgaaaactat 240
aggccgttag aaaaccccat cccccgggct gcccgcgact gcgggccgga aaggactcgt 300
atg tct att gac gag cag gtc tcc tcc tac tac aac atg ctg ctc aag 348
Met Ser Ile Asp Glu Gln Val Ser Ser Tyr Tyr Asn Met Leu Leu Lys
1 5 10 15
cgg aac gca gga gag ccg gag ttc cac cag gca gtc gca gaa gtt ctc 396
Arg Asn Ala Gly Glu Pro Glu Phe His Gln Ala Val Ala Glu Val Leu
20 25 30
gcc tcc ctc aag atc gtc ctg gag aag gat ccg cac tac gcg gac tac 444
Ala Ser Leu Lys Ile Val Leu Glu Lys Asp Pro His Tyr Ala Asp Tyr
35 40 45
ggc ctc atc cag cgc ctg tgc gag ccg gag cgc cag ctc atc ttc cgt 492
Gly Leu Ile Gln Arg Leu Cys Glu Pro Glu Arg Gln Leu Ile Phe Arg
50 55 60
gtg ccg tgg atc gac gac aac ggc gtg gta cag gtc aac cgt ggt ttc 540
Val Pro Trp Ile Asp Asp Asn Gly Val Val Gln Val Asn Arg Gly Phe
65 70 75 80
cga gta cag ttc aac tcc gcc ctc ggc ccg tac aag ggc ggc cta cgc 588
Arg Val Gln Phe Asn Ser Ala Leu Gly Pro Tyr Lys Gly Gly Leu Arg
85 90 95
ttc cac ccg tcc gtc aac ctg ggc atc atc aag ttc ctc ggc ttc gag 636
Phe His Pro Ser Val Asn Leu Gly Ile Ile Lys Phe Leu Gly Phe Glu
100 105 110
cag atc ttc aag aac tcc ctc acc ggc ctg ccc atc ggc ggc ggc aag 684
Gln Ile Phe Lys Asn Ser Leu Thr Gly Leu Pro Ile Gly Gly Gly Lys
115 120 125
ggc ggc tcc gac ttc gac ccg aag ggc aag tcc gag ctc gag atc atg 732
Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Leu Glu Ile Met
130 135 140
cgt ttc tgc cag tcc ttc atg acc gag ctg cac aag cac atc ggc gag 780
Arg Phe Cys Gln Ser Phe Met Thr Glu Leu His Lys His Ile Gly Glu
145 150 155 160
tac aag gac gtc ccc gcc ggt gac atc ggc gtc ggc ggc cgg gag atc 828
Tyr Lys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile
165 170 175
ggt ttc ctc ttc ggc cag tac cgt cgt ctg gcc ggc cag cac gag tcc 876
Gly Phe Leu Phe Gly Gln Tyr Arg Arg Leu Ala Gly Gln His Glu Ser
180 185 190
ggc gtc ctc acc ggc aag ggc ctc gcc tgg ggt ggc tcc ctc gtg cgc 924
Gly Val Leu Thr Gly Lys Gly Leu Ala Trp Gly Gly Ser Leu Val Arg
195 200 205
acc gag gcc acc ggc tac ggc gcc gtc tac ctc acg cag gag atg atg 972
Thr Glu Ala Thr Gly Tyr Gly Ala Val Tyr Leu Thr Gln Glu Met Met
210 215 220
aag gcc cac ggc gac tcc ctc tcc ggt tcg aag gtc atc gtc tcc ggc 1020
Lys Ala His Gly Asp Ser Leu Ser Gly Ser Lys Val Ile Val Ser Gly
225 230 235 240
tcc ggc aac gtc gcc atc tac gcc atc gag aag gcc cag gag ctc ggc 1068
Ser Gly Asn Val Ala Ile Tyr Ala Ile Glu Lys Ala Gln Glu Leu Gly
245 250 255
gcc acc gtc gtc ggc ttc tcc gac tcc tcc ggc tgg gtc tcc acc ccg 1116
Ala Thr Val Val Gly Phe Ser Asp Ser Ser Gly Trp Val Ser Thr Pro
260 265 270
aac ggc gtc gac gtc gag ctg ctc cac gac gtc aag gag aag cgc cgc 1164
Asn Gly Val Asp Val Glu Leu Leu His Asp Val Lys Glu Lys Arg Arg
275 280 285
gag cgc gtc tcc tgc tac gcc gag gag gcc acc ggc gcg acc ttc cac 1212
Glu Arg Val Ser Cys Tyr Ala Glu Glu Ala Thr Gly Ala Thr Phe His
290 295 300
gcc gag ggc tcc atc tgg gac ctc gcc gcc gac gtc gcc ctc ccg tgc 1260
Ala Glu Gly Ser Ile Trp Asp Leu Ala Ala Asp Val Ala Leu Pro Cys
305 310 315 320
gcc acc cag aac gag ctc aac ggc gac cac gcc cgc acc ctg gcc gac 1308
Ala Thr Gln Asn Glu Leu Asn Gly Asp His Ala Arg Thr Leu Ala Asp
325 330 335
aac ggc atc cgc tac gtc gcc gag ggc gcc aac atg ccc tcc acc ccg 1356
Asn Gly Ile Arg Tyr Val Ala Glu Gly Ala Asn Met Pro Ser Thr Pro
340 345 350
gag gcc atc gag gtc ttc cgt gag cgc ggt atc ttc ttc gcc ccg ggc 1404
Glu Ala Ile Glu Val Phe Arg Glu Arg Gly Ile Phe Phe Ala Pro Gly
355 360 365
aag gcc gcc aac gcc ggt ggc gtg gcc acc tcc gcc ctg gag atg cag 1452
Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Ala Leu Glu Met Gln
370 375 380
cag aac gcc tcc cgt gac tcc tgg tcc ttc gag tac acg gac cag cgc 1500
Gln Asn Ala Ser Arg Asp Ser Trp Ser Phe Glu Tyr Thr Asp Gln Arg
385 390 395 400
ctg cac aag atc atg tcc aag atc ttc cgc acc atg tcc cgc act gcc 1548
Leu His Lys Ile Met Ser Lys Ile Phe Arg Thr Met Ser Arg Thr Ala
405 410 415
gca gag tac ggc cac gag ggc aac tac gtc gtc ggc tcc aac atc gcc 1596
Ala Glu Tyr Gly His Glu Gly Asn Tyr Val Val Gly Ser Asn Ile Ala
420 425 430
ggc ttc aag aag gtc gcc gac gcg atg ctc gcg cag ggc gtc atc taa 1644
Gly Phe Lys Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile
435 440 445
ggctctccac aggattccct gaccccctgg ccctgccgca ccacacattg tgcggcaggg 1704
ccagtaatct ttgagtcatg tatagagtct tcgaagccct ggacgagctc aaccagaccg 1764
tggagcaggc ctacggcgtg cccatgacct ccaactgcat ggtgccgcgc aacgacaccc 1824
tcgcgcttct cgacgatctg cgtaacgccc tgcccgtcga gatcgacgac gcccaggatg 1884
tcctggacca gcgcgacgag atcctccgcg gggccgagga ccgggcccgc gacatgatcg 1944
<210> 124
<211> 447
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 124
Met Ser Ile Asp Glu Gln Val Ser Ser Tyr Tyr Asn Met Leu Leu Lys
1 5 10 15
Arg Asn Ala Gly Glu Pro Glu Phe His Gln Ala Val Ala Glu Val Leu
20 25 30
Ala Ser Leu Lys Ile Val Leu Glu Lys Asp Pro His Tyr Ala Asp Tyr
35 40 45
Gly Leu Ile Gln Arg Leu Cys Glu Pro Glu Arg Gln Leu Ile Phe Arg
50 55 60
Val Pro Trp Ile Asp Asp Asn Gly Val Val Gln Val Asn Arg Gly Phe
65 70 75 80
Arg Val Gln Phe Asn Ser Ala Leu Gly Pro Tyr Lys Gly Gly Leu Arg
85 90 95
Phe His Pro Ser Val Asn Leu Gly Ile Ile Lys Phe Leu Gly Phe Glu
100 105 110
Gln Ile Phe Lys Asn Ser Leu Thr Gly Leu Pro Ile Gly Gly Gly Lys
115 120 125
Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Leu Glu Ile Met
130 135 140
Arg Phe Cys Gln Ser Phe Met Thr Glu Leu His Lys His Ile Gly Glu
145 150 155 160
Tyr Lys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile
165 170 175
Gly Phe Leu Phe Gly Gln Tyr Arg Arg Leu Ala Gly Gln His Glu Ser
180 185 190
Gly Val Leu Thr Gly Lys Gly Leu Ala Trp Gly Gly Ser Leu Val Arg
195 200 205
Thr Glu Ala Thr Gly Tyr Gly Ala Val Tyr Leu Thr Gln Glu Met Met
210 215 220
Lys Ala His Gly Asp Ser Leu Ser Gly Ser Lys Val Ile Val Ser Gly
225 230 235 240
Ser Gly Asn Val Ala Ile Tyr Ala Ile Glu Lys Ala Gln Glu Leu Gly
245 250 255
Ala Thr Val Val Gly Phe Ser Asp Ser Ser Gly Trp Val Ser Thr Pro
260 265 270
Asn Gly Val Asp Val Glu Leu Leu His Asp Val Lys Glu Lys Arg Arg
275 280 285
Glu Arg Val Ser Cys Tyr Ala Glu Glu Ala Thr Gly Ala Thr Phe His
290 295 300
Ala Glu Gly Ser Ile Trp Asp Leu Ala Ala Asp Val Ala Leu Pro Cys
305 310 315 320
Ala Thr Gln Asn Glu Leu Asn Gly Asp His Ala Arg Thr Leu Ala Asp
325 330 335
Asn Gly Ile Arg Tyr Val Ala Glu Gly Ala Asn Met Pro Ser Thr Pro
340 345 350
Glu Ala Ile Glu Val Phe Arg Glu Arg Gly Ile Phe Phe Ala Pro Gly
355 360 365
Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Ala Leu Glu Met Gln
370 375 380
Gln Asn Ala Ser Arg Asp Ser Trp Ser Phe Glu Tyr Thr Asp Gln Arg
385 390 395 400
Leu His Lys Ile Met Ser Lys Ile Phe Arg Thr Met Ser Arg Thr Ala
405 410 415
Ala Glu Tyr Gly His Glu Gly Asn Tyr Val Val Gly Ser Asn Ile Ala
420 425 430
Gly Phe Lys Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile
435 440 445
<210> 125
<211> 2037
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1737)
<223> ген глутаминсинтетазы_1
<400> 125
agcgagccgg ggccggtctc ggggaggccg agtttctctc cggggtaccg gccgggtgcg 60
aaggggtcct cgtgttcgcc ggggatctgg gggccgtcga gccagctacg cttggggttc 120
gccataccgt agacattagc cgggggcgtg cgggcagggg aaactcgagt ttcctgcgac 180
tattgatcgg tcaacagata gcagtttaga atgagcgaca ggtaataggg agcggtgccc 240
gcacctgtag actggctgag ataccgcacg acctagagcg tcaacaacca aggagtcatc 300
gtg gcc ttc aac acg atc gaa gaa gtc gtc aag tac atc aag gat gaa 348
Val Ala Phe Asn Thr Ile Glu Glu Val Val Lys Tyr Ile Lys Asp Glu
1 5 10 15
aac gtc gag ttc gtc gac gtc cgc ttc acg gac gtc ccg ggc acc gag 396
Asn Val Glu Phe Val Asp Val Arg Phe Thr Asp Val Pro Gly Thr Glu
20 25 30
cac cac ttc acc atc ccc gcc tcg atg ttc gac gag gag gcg gcg gag 444
His His Phe Thr Ile Pro Ala Ser Met Phe Asp Glu Glu Ala Ala Glu
35 40 45
gaa ggc ctc gcc ttc gac ggc tcc tcc atc cgc ggc ttc acc acg atc 492
Glu Gly Leu Ala Phe Asp Gly Ser Ser Ile Arg Gly Phe Thr Thr Ile
50 55 60
gac gag tcc gac atg aac ctc ctg ccg gac ctc aag acc gcc aag ctc 540
Asp Glu Ser Asp Met Asn Leu Leu Pro Asp Leu Lys Thr Ala Lys Leu
65 70 75 80
gac ccc ttc cgc gag gcc aag acg ctg aac atc aag ttc ttc gtc cac 588
Asp Pro Phe Arg Glu Ala Lys Thr Leu Asn Ile Lys Phe Phe Val His
85 90 95
gac ccc ttc acc cgc gag ccc ttc tcc cgc gac ccc cgc aac gtc gcc 636
Asp Pro Phe Thr Arg Glu Pro Phe Ser Arg Asp Pro Arg Asn Val Ala
100 105 110
cgc aag gcc gag gag tac ctc gcc tcc acc ggc atc gcc gac acc tgc 684
Arg Lys Ala Glu Glu Tyr Leu Ala Ser Thr Gly Ile Ala Asp Thr Cys
115 120 125
ttc ttc ggc gcc gag gcc gag ttc tac ctc ttc gac acc gtc cgg ttc 732
Phe Phe Gly Ala Glu Ala Glu Phe Tyr Leu Phe Asp Thr Val Arg Phe
130 135 140
gag acc tcc acc aac ggc ggc ttc tac gag gtc gac tcc gac gag ggc 780
Glu Thr Ser Thr Asn Gly Gly Phe Tyr Glu Val Asp Ser Asp Glu Gly
145 150 155 160
tgg tgg aac cgc ggc aag gag gag cgt ctc gac ggc cgc cgc aac ctc 828
Trp Trp Asn Arg Gly Lys Glu Glu Arg Leu Asp Gly Arg Arg Asn Leu
165 170 175
ggc tac acc aac cgc gtc aag ggc gga tac ttc ccg gtc gcc ccc tac 876
Gly Tyr Thr Asn Arg Val Lys Gly Gly Tyr Phe Pro Val Ala Pro Tyr
180 185 190
gac cag acc gtg gac atc cgt gac tcc atg gtg cgc cac ctg cag aac 924
Asp Gln Thr Val Asp Ile Arg Asp Ser Met Val Arg His Leu Gln Asn
195 200 205
gcc gac ttc cac atc gag cgc ttc cac aag gag gtg ggc acg ggc ggc 972
Ala Asp Phe His Ile Glu Arg Phe His Lys Glu Val Gly Thr Gly Gly
210 215 220
cag cag gag atc aac tac cgc ttc aac acc ctg ctc cac gcc gcc gat 1020
Gln Gln Glu Ile Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp
225 230 235 240
gat ctg cag acc ttc aag tac atc atc aag aac acc gcc ttc aac gcc 1068
Asp Leu Gln Thr Phe Lys Tyr Ile Ile Lys Asn Thr Ala Phe Asn Ala
245 250 255
ggc aag acg gcc acg ttc atg ccc aag ccc atc gcc ggc gac aac ggt 1116
Gly Lys Thr Ala Thr Phe Met Pro Lys Pro Ile Ala Gly Asp Asn Gly
260 265 270
tcc ggc atg cac gcc cac cag tcg ctg tgg aag gac ggc gag ccg ctc 1164
Ser Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Glu Pro Leu
275 280 285
ttc cac gac gag tcc ggc tac gcc ggc ctg tcc gac atg gcc cgc tac 1212
Phe His Asp Glu Ser Gly Tyr Ala Gly Leu Ser Asp Met Ala Arg Tyr
290 295 300
tac atc ggc ggc atc ctc cac cac gcc ggc gcc gtc ctg gcc ttc acc 1260
Tyr Ile Gly Gly Ile Leu His His Ala Gly Ala Val Leu Ala Phe Thr
305 310 315 320
aac ccg acg ctg aac tcc tac cac cgc ctg gtc aag ggc ttc gag gca 1308
Asn Pro Thr Leu Asn Ser Tyr His Arg Leu Val Lys Gly Phe Glu Ala
325 330 335
ccg atc aac ctg gtg tac tcc cag cgc aac cgc tcc gcc gcg atc cgc 1356
Pro Ile Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg
340 345 350
atc ccg atc acc ggc tcc aac ccg aag gcc aag cgc atc gag ttc cgc 1404
Ile Pro Ile Thr Gly Ser Asn Pro Lys Ala Lys Arg Ile Glu Phe Arg
355 360 365
gcc ccg gac ccg tcc ggc aac ccg tac ctc ggc ttc gcc gcg atg atg 1452
Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Met Met
370 375 380
ctc gcc ggc ctc gac ggc atc aag aac cgc atc gag ccg atg gcc ccg 1500
Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Met Ala Pro
385 390 395 400
gtg gac aag gac ctc tac gag ctc ccg ccg gag gag gcc gcc gcc atc 1548
Val Asp Lys Asp Leu Tyr Glu Leu Pro Pro Glu Glu Ala Ala Ala Ile
405 410 415
ccg cag gcc ccg acc tcc ctc gag gcc tcc ctg gcc gcc ctg cag gag 1596
Pro Gln Ala Pro Thr Ser Leu Glu Ala Ser Leu Ala Ala Leu Gln Glu
420 425 430
gac aac gac ttc ctc acc gag ggt gac gtg ttc acc gag gac ctc atc 1644
Asp Asn Asp Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile
435 440 445
gag gcc tac atc cag ctc aag tac gac aac gag atc acc ccg gcc cgt 1692
Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Thr Pro Ala Arg
450 455 460
ctg cgc ccg acg ccg ctg gag ttc gag ctg tac tac gac tgc tga 1737
Leu Arg Pro Thr Pro Leu Glu Phe Glu Leu Tyr Tyr Asp Cys
465 470 475
ccatgccccc accgcaaccc ccgcgccgga gctttccgac gcgggggttg cgcgcctctc 1797
ccctgtcagc taactgacag taactatact ttccaccgtg aacatatccc tggcaggccc 1857
ccgtcgcctc gcatccctca tcctgtcact gttcctggcg ttgtccctgg cccccgcggc 1917
acacgccgcc accggcagcc cccaggaatt cgtcccctac accgacagcc acggcacaag 1977
ttcgaactac cacttctacc cctccggccg cgagaacgcc ggactcctcg tccacctcga 2037
<210> 126
<211> 478
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 126
Val Ala Phe Asn Thr Ile Glu Glu Val Val Lys Tyr Ile Lys Asp Glu
1 5 10 15
Asn Val Glu Phe Val Asp Val Arg Phe Thr Asp Val Pro Gly Thr Glu
20 25 30
His His Phe Thr Ile Pro Ala Ser Met Phe Asp Glu Glu Ala Ala Glu
35 40 45
Glu Gly Leu Ala Phe Asp Gly Ser Ser Ile Arg Gly Phe Thr Thr Ile
50 55 60
Asp Glu Ser Asp Met Asn Leu Leu Pro Asp Leu Lys Thr Ala Lys Leu
65 70 75 80
Asp Pro Phe Arg Glu Ala Lys Thr Leu Asn Ile Lys Phe Phe Val His
85 90 95
Asp Pro Phe Thr Arg Glu Pro Phe Ser Arg Asp Pro Arg Asn Val Ala
100 105 110
Arg Lys Ala Glu Glu Tyr Leu Ala Ser Thr Gly Ile Ala Asp Thr Cys
115 120 125
Phe Phe Gly Ala Glu Ala Glu Phe Tyr Leu Phe Asp Thr Val Arg Phe
130 135 140
Glu Thr Ser Thr Asn Gly Gly Phe Tyr Glu Val Asp Ser Asp Glu Gly
145 150 155 160
Trp Trp Asn Arg Gly Lys Glu Glu Arg Leu Asp Gly Arg Arg Asn Leu
165 170 175
Gly Tyr Thr Asn Arg Val Lys Gly Gly Tyr Phe Pro Val Ala Pro Tyr
180 185 190
Asp Gln Thr Val Asp Ile Arg Asp Ser Met Val Arg His Leu Gln Asn
195 200 205
Ala Asp Phe His Ile Glu Arg Phe His Lys Glu Val Gly Thr Gly Gly
210 215 220
Gln Gln Glu Ile Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp
225 230 235 240
Asp Leu Gln Thr Phe Lys Tyr Ile Ile Lys Asn Thr Ala Phe Asn Ala
245 250 255
Gly Lys Thr Ala Thr Phe Met Pro Lys Pro Ile Ala Gly Asp Asn Gly
260 265 270
Ser Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Glu Pro Leu
275 280 285
Phe His Asp Glu Ser Gly Tyr Ala Gly Leu Ser Asp Met Ala Arg Tyr
290 295 300
Tyr Ile Gly Gly Ile Leu His His Ala Gly Ala Val Leu Ala Phe Thr
305 310 315 320
Asn Pro Thr Leu Asn Ser Tyr His Arg Leu Val Lys Gly Phe Glu Ala
325 330 335
Pro Ile Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg
340 345 350
Ile Pro Ile Thr Gly Ser Asn Pro Lys Ala Lys Arg Ile Glu Phe Arg
355 360 365
Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Met Met
370 375 380
Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Met Ala Pro
385 390 395 400
Val Asp Lys Asp Leu Tyr Glu Leu Pro Pro Glu Glu Ala Ala Ala Ile
405 410 415
Pro Gln Ala Pro Thr Ser Leu Glu Ala Ser Leu Ala Ala Leu Gln Glu
420 425 430
Asp Asn Asp Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile
435 440 445
Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Thr Pro Ala Arg
450 455 460
Leu Arg Pro Thr Pro Leu Glu Phe Glu Leu Tyr Tyr Asp Cys
465 470 475
<210> 127
<211> 2037
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1737)
<223> ген глутаминсинтетазы_2
<400> 127
catccccggc aaccgctggt gggacatgac cgaggagggc atcctgccga aggagcgcct 60
ggtgaacatc ggcgacatcg ccgccggccg tgccctgggc cgcgagaacg acgagcagat 120
cttctgctac tccatcggcg gcatgcccgt ggaggacgtc gcctgggcct gggacgtcta 180
ccacaacgcc ctggagaagg gcatcggcac caggctcaac ctgtgggaca cccccaccct 240
caagtaagcc cctcaagcaa gacacctgaa cacaacaatc taggagccct gacacctgtc 300
gtg tcc ttc cag tcc acc acc gac ctg atc gac ttc atg cgc gat gag 348
Val Ser Phe Gln Ser Thr Thr Asp Leu Ile Asp Phe Met Arg Asp Glu
1 5 10 15
ggg gtg gag tac ctg gac atc cgt ttc acc gat gtc ccc ggc aca gaa 396
Gly Val Glu Tyr Leu Asp Ile Arg Phe Thr Asp Val Pro Gly Thr Glu
20 25 30
cac gcg ctg acg gtt ccc gcc gcg gcg ctg acc gag gag acc gcg gcg 444
His Ala Leu Thr Val Pro Ala Ala Ala Leu Thr Glu Glu Thr Ala Ala
35 40 45
gag ggc ttc gcc ttc gac ggc tcg tcg atc gca ggt ttc acc tcg gtc 492
Glu Gly Phe Ala Phe Asp Gly Ser Ser Ile Ala Gly Phe Thr Ser Val
50 55 60
gac gag tcc gac atg acc ctc atg ccg gac gtc tcg acg gcc tac gtg 540
Asp Glu Ser Asp Met Thr Leu Met Pro Asp Val Ser Thr Ala Tyr Val
65 70 75 80
gac ccc ttc cgt cac cgg aag acg gtg aac atg cag ttc ttc gtc cac 588
Asp Pro Phe Arg His Arg Lys Thr Val Asn Met Gln Phe Phe Val His
85 90 95
gac ccc ttc acc cgc gag gcg ttc tcc cgc gat ccg cgc aac atc gcc 636
Asp Pro Phe Thr Arg Glu Ala Phe Ser Arg Asp Pro Arg Asn Ile Ala
100 105 110
cgc aag gcg gag gag tac atg cgt gcc tcc ggc atc gcc gac gag tgc 684
Arg Lys Ala Glu Glu Tyr Met Arg Ala Ser Gly Ile Ala Asp Glu Cys
115 120 125
tac atc ggc gcc gag gcc gag ttc ttc gtc ttc gac tcg gtc cgc tac 732
Tyr Ile Gly Ala Glu Ala Glu Phe Phe Val Phe Asp Ser Val Arg Tyr
130 135 140
tcc acc gac gtc cac cgt tcc ttc cac cag gtc gac tcc gac gag ggc 780
Ser Thr Asp Val His Arg Ser Phe His Gln Val Asp Ser Asp Glu Gly
145 150 155 160
tgg tgg aac tcc gac gcg gag acc atg atc gac ggt tcc ccg aac ctg 828
Trp Trp Asn Ser Asp Ala Glu Thr Met Ile Asp Gly Ser Pro Asn Leu
165 170 175
ggc aac aag atc cgc gtc aac gac ggc tac ttc ccc gtc gcc ccc tac 876
Gly Asn Lys Ile Arg Val Asn Asp Gly Tyr Phe Pro Val Ala Pro Tyr
180 185 190
gac aag acg atc ccg gtg cgc gac gag atc gcg cac aac ctc tcg cag 924
Asp Lys Thr Ile Pro Val Arg Asp Glu Ile Ala His Asn Leu Ser Gln
195 200 205
gtc ggt ttc gag atc gag cgt ttc cac cac gag gtc tcc acg ggt ggc 972
Val Gly Phe Glu Ile Glu Arg Phe His His Glu Val Ser Thr Gly Gly
210 215 220
atc cag gag gtg aac tac cgc ttc aac acc ctc ctg cac gcg gcg gat 1020
Ile Gln Glu Val Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp
225 230 235 240
gac ctg cag aag ttc aag tac atc gtg aag aac acc gcc gac gcc cac 1068
Asp Leu Gln Lys Phe Lys Tyr Ile Val Lys Asn Thr Ala Asp Ala His
245 250 255
ggc aag gtc gcc acc ttc atg ccg aag ccg ctt ctc gac gac ggc ggc 1116
Gly Lys Val Ala Thr Phe Met Pro Lys Pro Leu Leu Asp Asp Gly Gly
260 265 270
tgc ggc atg cac gcc cac cag tcc ctg tgg aag gac ggc cga ccg ctg 1164
Cys Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Arg Pro Leu
275 280 285
ttc cac gac gag gaa ggt tac gcc ggc ctc tcc gag acc gcc cgc cac 1212
Phe His Asp Glu Glu Gly Tyr Ala Gly Leu Ser Glu Thr Ala Arg His
290 295 300
tac atc ggt ggc ctg ctg gcc cac gcc ccg gcg gtc ctg gcg ttc acc 1260
Tyr Ile Gly Gly Leu Leu Ala His Ala Pro Ala Val Leu Ala Phe Thr
305 310 315 320
aac ccg acg gtc aac tcc tac cgt cgc ctc tac tcc ggt ttc gag gcc 1308
Asn Pro Thr Val Asn Ser Tyr Arg Arg Leu Tyr Ser Gly Phe Glu Ala
325 330 335
ccg gtg aac ctg gtg tac tcc cag cgc aac cgt tcc gcc gcg atc cgt 1356
Pro Val Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg
340 345 350
atc ccg gtg acc ggc ccc tcc gcc gcc gcc aag cgc atc gag ttc cgc 1404
Ile Pro Val Thr Gly Pro Ser Ala Ala Ala Lys Arg Ile Glu Phe Arg
355 360 365
gcc ccg gac ccc tcc ggc aac ccc tac ctc ggt ttc gcg gcc cag ctg 1452
Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Gln Leu
370 375 380
ctg gcc ggt ctg gac ggc atc aag aac cgc atc gag ccg ggc gac ccg 1500
Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Gly Asp Pro
385 390 395 400
gtg gac aag gac ctc tac gag ctg gag ccg gag gag gcc gac aag gtg 1548
Val Asp Lys Asp Leu Tyr Glu Leu Glu Pro Glu Glu Ala Asp Lys Val
405 410 415
ccg cgc gtc ccg cac tcc ctg gag tcc tcg ctg tac gcc ctg gag aac 1596
Pro Arg Val Pro His Ser Leu Glu Ser Ser Leu Tyr Ala Leu Glu Asn
420 425 430
gac cac gag ttc ctc acc gag ggc gac gtg ttc acc gag gac ctc atc 1644
Asp His Glu Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile
435 440 445
gag gcc tac atc cag ctg aag tac gac aac gag atc aac ccg atg cgc 1692
Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Asn Pro Met Arg
450 455 460
caa ggc ccg acg ccg aag gag ttc gag ctc tac ttc aac tgc tag 1737
Gln Gly Pro Thr Pro Lys Glu Phe Glu Leu Tyr Phe Asn Cys
465 470 475
gttcagcagg catgcggcgc agttacgtcc tcggggcgac agagcacacg gacctccggg 1797
gcatccggcg ggtgctcgca cgctaccgct acgacgcgcc gtgggtgctt ctcgacgccc 1857
gccccgtcct cgaggtctcc tggttcgggg agggcgcggt gtcgttctac gccaccactc 1917
ccccgctgcc tcccgacccc gccctggccc gcctcctctt cgacctcggg tcctgcggac 1977
tgctgctcgg ggtgtcgccg gggccgccgg acatcgtcat ctgcggtggt cacagtacgg 2037
<210> 128
<211> 478
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 128
Val Ser Phe Gln Ser Thr Thr Asp Leu Ile Asp Phe Met Arg Asp Glu
1 5 10 15
Gly Val Glu Tyr Leu Asp Ile Arg Phe Thr Asp Val Pro Gly Thr Glu
20 25 30
His Ala Leu Thr Val Pro Ala Ala Ala Leu Thr Glu Glu Thr Ala Ala
35 40 45
Glu Gly Phe Ala Phe Asp Gly Ser Ser Ile Ala Gly Phe Thr Ser Val
50 55 60
Asp Glu Ser Asp Met Thr Leu Met Pro Asp Val Ser Thr Ala Tyr Val
65 70 75 80
Asp Pro Phe Arg His Arg Lys Thr Val Asn Met Gln Phe Phe Val His
85 90 95
Asp Pro Phe Thr Arg Glu Ala Phe Ser Arg Asp Pro Arg Asn Ile Ala
100 105 110
Arg Lys Ala Glu Glu Tyr Met Arg Ala Ser Gly Ile Ala Asp Glu Cys
115 120 125
Tyr Ile Gly Ala Glu Ala Glu Phe Phe Val Phe Asp Ser Val Arg Tyr
130 135 140
Ser Thr Asp Val His Arg Ser Phe His Gln Val Asp Ser Asp Glu Gly
145 150 155 160
Trp Trp Asn Ser Asp Ala Glu Thr Met Ile Asp Gly Ser Pro Asn Leu
165 170 175
Gly Asn Lys Ile Arg Val Asn Asp Gly Tyr Phe Pro Val Ala Pro Tyr
180 185 190
Asp Lys Thr Ile Pro Val Arg Asp Glu Ile Ala His Asn Leu Ser Gln
195 200 205
Val Gly Phe Glu Ile Glu Arg Phe His His Glu Val Ser Thr Gly Gly
210 215 220
Ile Gln Glu Val Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp
225 230 235 240
Asp Leu Gln Lys Phe Lys Tyr Ile Val Lys Asn Thr Ala Asp Ala His
245 250 255
Gly Lys Val Ala Thr Phe Met Pro Lys Pro Leu Leu Asp Asp Gly Gly
260 265 270
Cys Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Arg Pro Leu
275 280 285
Phe His Asp Glu Glu Gly Tyr Ala Gly Leu Ser Glu Thr Ala Arg His
290 295 300
Tyr Ile Gly Gly Leu Leu Ala His Ala Pro Ala Val Leu Ala Phe Thr
305 310 315 320
Asn Pro Thr Val Asn Ser Tyr Arg Arg Leu Tyr Ser Gly Phe Glu Ala
325 330 335
Pro Val Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg
340 345 350
Ile Pro Val Thr Gly Pro Ser Ala Ala Ala Lys Arg Ile Glu Phe Arg
355 360 365
Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Gln Leu
370 375 380
Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Gly Asp Pro
385 390 395 400
Val Asp Lys Asp Leu Tyr Glu Leu Glu Pro Glu Glu Ala Asp Lys Val
405 410 415
Pro Arg Val Pro His Ser Leu Glu Ser Ser Leu Tyr Ala Leu Glu Asn
420 425 430
Asp His Glu Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile
435 440 445
Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Asn Pro Met Arg
450 455 460
Gln Gly Pro Thr Pro Lys Glu Phe Glu Leu Tyr Phe Asn Cys
465 470 475
<210> 129
<211> 2241
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1941)
<223> ген глутаматсинтазы
<400> 129
ctggggttcc gcgtcgaagg acaccatcaa actggcccgc ctggccaccc agaccggcct 60
gttccccgtg tacgaggcgg agtacggcga gatcacctcg gtgaagaaga tccgccgccc 120
cgccccggtc gaggactacc tccgcctcca gcgccgctac gcgcacctgt tccggaagga 180
cgcggacccg tccgtcatcg agcggctcca ggcgctggcg gaccgcaaca tccgccgcta 240
caacctcatc gacgaagaga ccctcgacga gttcgtcagg ttcgaaggag acgacgcaca 300
gtg agt acc ggt aac cac gtc aac atc ggc atg ccc ttc gcc atc acc 348
Val Ser Thr Gly Asn His Val Asn Ile Gly Met Pro Phe Ala Ile Thr
1 5 10 15
ctg cag acc ggc tcc tcc ctg gcg aac aag acc ggc tcc tgg cgc agc 396
Leu Gln Thr Gly Ser Ser Leu Ala Asn Lys Thr Gly Ser Trp Arg Ser
20 25 30
gag cgg ccc gtg tac gtc gac ctg ctg ccg ccc tgc aac aag gcg tgt 444
Glu Arg Pro Val Tyr Val Asp Leu Leu Pro Pro Cys Asn Lys Ala Cys
35 40 45
ccc gcc ggc ggg aac atc cag ggc tgg ctg tac cac gcc gag gag ggc 492
Pro Ala Gly Gly Asn Ile Gln Gly Trp Leu Tyr His Ala Glu Glu Gly
50 55 60
gac tac gag tcc gcc tgg cgc gag atc atg gtg gac aac ccg ctg ccc 540
Asp Tyr Glu Ser Ala Trp Arg Glu Ile Met Val Asp Asn Pro Leu Pro
65 70 75 80
gcc gtc atg ggg cgt gtc tgc tac cac acc tgc cag acc gcc tgt aac 588
Ala Val Met Gly Arg Val Cys Tyr His Thr Cys Gln Thr Ala Cys Asn
85 90 95
cgc gcg gag gtc gac gag gcc gtc ggc atc aac gcg gtc gag cgc ttc 636
Arg Ala Glu Val Asp Glu Ala Val Gly Ile Asn Ala Val Glu Arg Phe
100 105 110
ctc ggg gac cgt gcc atc gag gag ggg tgg cag gtc gaa ccc gtc aag 684
Leu Gly Asp Arg Ala Ile Glu Glu Gly Trp Gln Val Glu Pro Val Lys
115 120 125
gac ccc tcc ggc cgg agg gtc ctc gtc gtg ggt tcc ggc ccc tcc ggc 732
Asp Pro Ser Gly Arg Arg Val Leu Val Val Gly Ser Gly Pro Ser Gly
130 135 140
ctg tcc gcg gcc tac cac ctc gcc cga ctc ggc cac gag gtg acg gtc 780
Leu Ser Ala Ala Tyr His Leu Ala Arg Leu Gly His Glu Val Thr Val
145 150 155 160
cgc gac cgg ggt gag cgg ccg ggc ggc atg atg cgc tac ggc atc ccc 828
Arg Asp Arg Gly Glu Arg Pro Gly Gly Met Met Arg Tyr Gly Ile Pro
165 170 175
tcc tac cgc ctg ccc cgc cac atc ctc gac gcc gag atc gcg cgc atc 876
Ser Tyr Arg Leu Pro Arg His Ile Leu Asp Ala Glu Ile Ala Arg Ile
180 185 190
gcg gcc ctg ggc gtc acc ttc gag cag ggg gtc acc gtc acc gac gtc 924
Ala Ala Leu Gly Val Thr Phe Glu Gln Gly Val Thr Val Thr Asp Val
195 200 205
gag tcc gaa ctc gag ctt ttc gac gcc gtc ttc acc gcc gtc ggt gcc 972
Glu Ser Glu Leu Glu Leu Phe Asp Ala Val Phe Thr Ala Val Gly Ala
210 215 220
cag ctg ggc cgc aac gtg gag gtg ccc gcc ggg tcg gcg gcg acc atc 1020
Gln Leu Gly Arg Asn Val Glu Val Pro Ala Gly Ser Ala Ala Thr Ile
225 230 235 240
atg aac gcc gtt gac ctg ctg cgt gac acc gag gcc ggg gag gcg ccg 1068
Met Asn Ala Val Asp Leu Leu Arg Asp Thr Glu Ala Gly Glu Ala Pro
245 250 255
ctg ctc ggt cgt cgg gtc gtg gtc tac ggc ggc ggc aac aca gcc gtc 1116
Leu Leu Gly Arg Arg Val Val Val Tyr Gly Gly Gly Asn Thr Ala Val
260 265 270
gac gcc gcc cgc acc gcg cgt cgt ctc ggg gcg acc gag tcg gtc atc 1164
Asp Ala Ala Arg Thr Ala Arg Arg Leu Gly Ala Thr Glu Ser Val Ile
275 280 285
ctc tac cgc cgc acc cgc gac cac atg agc gcc cac gac tcc gag gtc 1212
Leu Tyr Arg Arg Thr Arg Asp His Met Ser Ala His Asp Ser Glu Val
290 295 300
atg gag gcc gag gag gag ggc atc acc atg cgc tgg ctg tcc acc gtc 1260
Met Glu Ala Glu Glu Glu Gly Ile Thr Met Arg Trp Leu Ser Thr Val
305 310 315 320
gac cac gtc gag gag ggc tcc atc agg atc gag aag atg gac atc gac 1308
Asp His Val Glu Glu Gly Ser Ile Arg Ile Glu Lys Met Asp Ile Asp
325 330 335
gcc gac ggc cac ctc acc ccg acc ggt gag tac gag gag ctc gcc gcc 1356
Ala Asp Gly His Leu Thr Pro Thr Gly Glu Tyr Glu Glu Leu Ala Ala
340 345 350
gac tcc ctc gtc atg gcg ctg ggc cag gag tcg gac ctc tcc ctg ctc 1404
Asp Ser Leu Val Met Ala Leu Gly Gln Glu Ser Asp Leu Ser Leu Leu
355 360 365
gag ggc gtg gag ggg gtg gag atc tcc cgc ggc gtc gtc gcc gtc gac 1452
Glu Gly Val Glu Gly Val Glu Ile Ser Arg Gly Val Val Ala Val Asp
370 375 380
gag cag atg atg acc gga cgt gcg ggc gtc ttc gcc ggc ggc gac atg 1500
Glu Gln Met Met Thr Gly Arg Ala Gly Val Phe Ala Gly Gly Asp Met
385 390 395 400
gtg ccg agc gag aag aac gtc acc gtc gcc atc ggc cac ggc aag aag 1548
Val Pro Ser Glu Lys Asn Val Thr Val Ala Ile Gly His Gly Lys Lys
405 410 415
gcg gcc cgg tac atc gac ggt tgg ctc cgt ggc gaa ccc tac gtg ccc 1596
Ala Ala Arg Tyr Ile Asp Gly Trp Leu Arg Gly Glu Pro Tyr Val Pro
420 425 430
gcc gtc aag cct gca gac gcg act ctc gac cgt atg gag acc tgg tac 1644
Ala Val Lys Pro Ala Asp Ala Thr Leu Asp Arg Met Glu Thr Trp Tyr
435 440 445
tac tca gag gcc ccg cac cag gtg cgc gaa cgc ctc gag ggc gcc cgc 1692
Tyr Ser Glu Ala Pro His Gln Val Arg Glu Arg Leu Glu Gly Ala Arg
450 455 460
cgc gcc tcc acc ttt gac gag gtc gtc cgg gga ctc gac gcc gac tcc 1740
Arg Ala Ser Thr Phe Asp Glu Val Val Arg Gly Leu Asp Ala Asp Ser
465 470 475 480
gcc ctg ctc gag gca cgc cgc tgc atg tcg tgc ggc aac tgc ttc ggc 1788
Ala Leu Leu Glu Ala Arg Arg Cys Met Ser Cys Gly Asn Cys Phe Gly
485 490 495
tgc gac aac tgc ttc ggc gtc tgc ccg gac aac gcg gtc acc aaa ttg 1836
Cys Asp Asn Cys Phe Gly Val Cys Pro Asp Asn Ala Val Thr Lys Leu
500 505 510
gcg ccg ggt gag tac gag ttc aag tac gac tac tgc aag ggc tgc ggc 1884
Ala Pro Gly Glu Tyr Glu Phe Lys Tyr Asp Tyr Cys Lys Gly Cys Gly
515 520 525
atc tgc gcg gag gag tgc ccg tgc ggc gcg atc tcc atg att ccc gag 1932
Ile Cys Ala Glu Glu Cys Pro Cys Gly Ala Ile Ser Met Ile Pro Glu
530 535 540
gag aac tga ggagaactga ccgcccgcaa caagttagag tatgggcacc 1981
Glu Asn
545
aacgtcgtcc cctgaccaga aggatcccca tgcgccgcac cctcatcgcc ctcggcaccg 2041
ccaccgccct caccctgggg gcggtcccgg ccgcccacgc ccagcccgcg aacgccgtca 2101
ccgcccagat cgaggagctg ccggccgaca tgcgggcagg ctcctccggc gcggagaagc 2161
tctcctccgc ctccaccgag gccgagaagc aggagggtgc cctgctgctg ggacgcgact 2221
ggctgctcgg tttcgccgcg 2241
<210> 130
<211> 546
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 130
Val Ser Thr Gly Asn His Val Asn Ile Gly Met Pro Phe Ala Ile Thr
1 5 10 15
Leu Gln Thr Gly Ser Ser Leu Ala Asn Lys Thr Gly Ser Trp Arg Ser
20 25 30
Glu Arg Pro Val Tyr Val Asp Leu Leu Pro Pro Cys Asn Lys Ala Cys
35 40 45
Pro Ala Gly Gly Asn Ile Gln Gly Trp Leu Tyr His Ala Glu Glu Gly
50 55 60
Asp Tyr Glu Ser Ala Trp Arg Glu Ile Met Val Asp Asn Pro Leu Pro
65 70 75 80
Ala Val Met Gly Arg Val Cys Tyr His Thr Cys Gln Thr Ala Cys Asn
85 90 95
Arg Ala Glu Val Asp Glu Ala Val Gly Ile Asn Ala Val Glu Arg Phe
100 105 110
Leu Gly Asp Arg Ala Ile Glu Glu Gly Trp Gln Val Glu Pro Val Lys
115 120 125
Asp Pro Ser Gly Arg Arg Val Leu Val Val Gly Ser Gly Pro Ser Gly
130 135 140
Leu Ser Ala Ala Tyr His Leu Ala Arg Leu Gly His Glu Val Thr Val
145 150 155 160
Arg Asp Arg Gly Glu Arg Pro Gly Gly Met Met Arg Tyr Gly Ile Pro
165 170 175
Ser Tyr Arg Leu Pro Arg His Ile Leu Asp Ala Glu Ile Ala Arg Ile
180 185 190
Ala Ala Leu Gly Val Thr Phe Glu Gln Gly Val Thr Val Thr Asp Val
195 200 205
Glu Ser Glu Leu Glu Leu Phe Asp Ala Val Phe Thr Ala Val Gly Ala
210 215 220
Gln Leu Gly Arg Asn Val Glu Val Pro Ala Gly Ser Ala Ala Thr Ile
225 230 235 240
Met Asn Ala Val Asp Leu Leu Arg Asp Thr Glu Ala Gly Glu Ala Pro
245 250 255
Leu Leu Gly Arg Arg Val Val Val Tyr Gly Gly Gly Asn Thr Ala Val
260 265 270
Asp Ala Ala Arg Thr Ala Arg Arg Leu Gly Ala Thr Glu Ser Val Ile
275 280 285
Leu Tyr Arg Arg Thr Arg Asp His Met Ser Ala His Asp Ser Glu Val
290 295 300
Met Glu Ala Glu Glu Glu Gly Ile Thr Met Arg Trp Leu Ser Thr Val
305 310 315 320
Asp His Val Glu Glu Gly Ser Ile Arg Ile Glu Lys Met Asp Ile Asp
325 330 335
Ala Asp Gly His Leu Thr Pro Thr Gly Glu Tyr Glu Glu Leu Ala Ala
340 345 350
Asp Ser Leu Val Met Ala Leu Gly Gln Glu Ser Asp Leu Ser Leu Leu
355 360 365
Glu Gly Val Glu Gly Val Glu Ile Ser Arg Gly Val Val Ala Val Asp
370 375 380
Glu Gln Met Met Thr Gly Arg Ala Gly Val Phe Ala Gly Gly Asp Met
385 390 395 400
Val Pro Ser Glu Lys Asn Val Thr Val Ala Ile Gly His Gly Lys Lys
405 410 415
Ala Ala Arg Tyr Ile Asp Gly Trp Leu Arg Gly Glu Pro Tyr Val Pro
420 425 430
Ala Val Lys Pro Ala Asp Ala Thr Leu Asp Arg Met Glu Thr Trp Tyr
435 440 445
Tyr Ser Glu Ala Pro His Gln Val Arg Glu Arg Leu Glu Gly Ala Arg
450 455 460
Arg Ala Ser Thr Phe Asp Glu Val Val Arg Gly Leu Asp Ala Asp Ser
465 470 475 480
Ala Leu Leu Glu Ala Arg Arg Cys Met Ser Cys Gly Asn Cys Phe Gly
485 490 495
Cys Asp Asn Cys Phe Gly Val Cys Pro Asp Asn Ala Val Thr Lys Leu
500 505 510
Ala Pro Gly Glu Tyr Glu Phe Lys Tyr Asp Tyr Cys Lys Gly Cys Gly
515 520 525
Ile Cys Ala Glu Glu Cys Pro Cys Gly Ala Ile Ser Met Ile Pro Glu
530 535 540
Glu Asn
545
<210> 131
<211> 2817
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2517)
<223> ген изоцитратдегидрогеназы
<400> 131
cgccgacgta gtgccagccc gcgtcgaggg cgggctgcaa cgccttgacg gactcctcga 60
cggtcgcgcg cacctcctgc aggagcacca cgtcagccgg ggactgctcc agccaggcca 120
gcatgcccgg attgtcctcg ttgcgctgct tgacggcggc gcggattcca ttgacgttga 180
cggaggtgat ggtcagactc ataggaacca ccatatcgcc gttggtggca gggcccctgc 240
cccgccgggt atccttagcc gttgacactg ccgagattga agaaagcgtt agggagttac 300
atg gca aag atc atc tgg acc cgc acc gac gag gcc ccg ctg ctc gcg 348
Met Ala Lys Ile Ile Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala
1 5 10 15
acc tat tcg ttc aag cct gtt gtt gag gcc ttc gcc gcc acc gcg ggt 396
Thr Tyr Ser Phe Lys Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly
20 25 30
atc gat gtc gag acc cgc gac atc tcc ctg gcc ggc cgc atc ctg gcc 444
Ile Asp Val Glu Thr Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala
35 40 45
cag ttc ccg gac cgc ctc acc gag gag cag cga gtc gct gac gcc ctc 492
Gln Phe Pro Asp Arg Leu Thr Glu Glu Gln Arg Val Ala Asp Ala Leu
50 55 60
acc gag ctc ggc gac ctg gcc aag acc ccg gaa gcc aac atc atc aag 540
Thr Glu Leu Gly Asp Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys
65 70 75 80
ctc ccg aac atc tcg gcg tcc gtc ccg cag ctc aag gca gcc atc aag 588
Leu Pro Asn Ile Ser Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys
85 90 95
gag ctg cag gac cag gga tac gac ctc ccc gac tac gtc gac tcc ccg 636
Glu Leu Gln Asp Gln Gly Tyr Asp Leu Pro Asp Tyr Val Asp Ser Pro
100 105 110
gag acc gac gag gag aag gac gcc cgc aag cgc tac gac gta gta aag 684
Glu Thr Asp Glu Glu Lys Asp Ala Arg Lys Arg Tyr Asp Val Val Lys
115 120 125
ggt tcc gct gtg aac ccg gta ctc cgt gag ggc aac tcc gac cgt cgt 732
Gly Ser Ala Val Asn Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg
130 135 140
gcc ccg gag gcc gtg aag aac ttc gtc cgc aag aac ccg cac cgc atg 780
Ala Pro Glu Ala Val Lys Asn Phe Val Arg Lys Asn Pro His Arg Met
145 150 155 160
ggt gag tgg tcc gcc gac tcc aag acc aac gtc gcc acc atg gac tcc 828
Gly Glu Trp Ser Ala Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ser
165 170 175
gac gac ttc cgt cac aac gag cag tcc gtc atc ctc ccg gcc gag gac 876
Asp Asp Phe Arg His Asn Glu Gln Ser Val Ile Leu Pro Ala Glu Asp
180 185 190
acc ctc tcc ttc gtc cac gtc gcc acc gac ggc acc gag acc aag ctc 924
Thr Leu Ser Phe Val His Val Ala Thr Asp Gly Thr Glu Thr Lys Leu
195 200 205
cgc gac gac ctc aag gtc ctc gag ggc gag gtc gtc gac ggc acc gtc 972
Arg Asp Asp Leu Lys Val Leu Glu Gly Glu Val Val Asp Gly Thr Val
210 215 220
atg cgc gcc gcc gcc ctg cag gag ttc ctg gcc gag cag gtc aag cgc 1020
Met Arg Ala Ala Ala Leu Gln Glu Phe Leu Ala Glu Gln Val Lys Arg
225 230 235 240
gcc cag gac gag ggc atc ctg ttc tcc gtc cac ctc aag gcc acc atg 1068
Ala Gln Asp Glu Gly Ile Leu Phe Ser Val His Leu Lys Ala Thr Met
245 250 255
atg aag gtc tcc gac ccg atc atc ttc ggc tac gtc gtc cgc gcc ttc 1116
Met Lys Val Ser Asp Pro Ile Ile Phe Gly Tyr Val Val Arg Ala Phe
260 265 270
ttc gcc gac gtc tac gag aag tac ggc gag gag ctg ctg gcc gcc ggt 1164
Phe Ala Asp Val Tyr Glu Lys Tyr Gly Glu Glu Leu Leu Ala Ala Gly
275 280 285
ctc gac ggc gag aac ggc ctc ggc gcc atc ttc tcc gga ctc gag gaa 1212
Leu Asp Gly Glu Asn Gly Leu Gly Ala Ile Phe Ser Gly Leu Glu Glu
290 295 300
ctg gag aac ggc gag gag atc cgc cgt gcc ttc gac gag gcc ctc gag 1260
Leu Glu Asn Gly Glu Glu Ile Arg Arg Ala Phe Asp Glu Ala Leu Glu
305 310 315 320
aac ggc cct gac ctg gcg atg gtg aac tcc gac aag ggc atc acc aac 1308
Asn Gly Pro Asp Leu Ala Met Val Asn Ser Asp Lys Gly Ile Thr Asn
325 330 335
ctg cac gtc ccg tcc gac gtc atc gtc gac gcc tcc atg ccc gcc atg 1356
Leu His Val Pro Ser Asp Val Ile Val Asp Ala Ser Met Pro Ala Met
340 345 350
atc cgc acc tcc ggc cag atg tgg aac aag gac gac aag acc cag gac 1404
Ile Arg Thr Ser Gly Gln Met Trp Asn Lys Asp Asp Lys Thr Gln Asp
355 360 365
acc ctg gcc gtc atc ccg gac tcc tcc tac gcc ggc gtc ttc cag acc 1452
Thr Leu Ala Val Ile Pro Asp Ser Ser Tyr Ala Gly Val Phe Gln Thr
370 375 380
gtc atc gac gac tgc aag gcc aac ggc gcc ttc gac ccg acc acc atg 1500
Val Ile Asp Asp Cys Lys Ala Asn Gly Ala Phe Asp Pro Thr Thr Met
385 390 395 400
ggc acc gtc ccg aac gtc ggc ctc atg gcc cag aag gcc gag gag tac 1548
Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr
405 410 415
ggc tcc cac gac aag acc ttc cgc atc gcc gcg gac ggc aag gtc gag 1596
Gly Ser His Asp Lys Thr Phe Arg Ile Ala Ala Asp Gly Lys Val Glu
420 425 430
gtc cgc aac tcc gcc ggt gac gtc ctg ctc gag cac gag gtc tcc gag 1644
Val Arg Asn Ser Ala Gly Asp Val Leu Leu Glu His Glu Val Ser Glu
435 440 445
ggc gac atc tgg cgc gcc tgc cag acc aag gac gcc ccg atc cag gac 1692
Gly Asp Ile Trp Arg Ala Cys Gln Thr Lys Asp Ala Pro Ile Gln Asp
450 455 460
tgg gtc aag ctg gcc gtc gac cgc gcc cgc atc tcc ggc atg ccg gcc 1740
Trp Val Lys Leu Ala Val Asp Arg Ala Arg Ile Ser Gly Met Pro Ala
465 470 475 480
gtc ttc tgg ctg gac ccg gag cgc gcc cac gac cgc aac ctc acc acc 1788
Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp Arg Asn Leu Thr Thr
485 490 495
ctg gtg gag aag tac ctg gcc gac cac gac acc gag ggc ctg gac atc 1836
Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile
500 505 510
cgc atc ctc tcc ccg gtc gag gcg acc cag ctg tcc gtc gac cgc atc 1884
Arg Ile Leu Ser Pro Val Glu Ala Thr Gln Leu Ser Val Asp Arg Ile
515 520 525
cgc cgc ggc gag gac acc atc tcc gtg acc ggt aac gtc ctg cgt gac 1932
Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp
530 535 540
tac aac acc gac ctc ttc ccg atc ctc gag ctg ggc acc tcc gcc aag 1980
Tyr Asn Thr Asp Leu Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys
545 550 555 560
atg ctc tcc gtc gtg ccg ctg atg gcc ggt ggc ggc ctc ttc gag acc 2028
Met Leu Ser Val Val Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr
565 570 575
ggt gcc ggt ggc tcc gcc ccg aag cac gtc cag cag gtc gtc gag gag 2076
Gly Ala Gly Gly Ser Ala Pro Lys His Val Gln Gln Val Val Glu Glu
580 585 590
aac cac ctg cgc tgg gac tcc ctc ggt gag ttc ctc gcc ctg gcc gag 2124
Asn His Leu Arg Trp Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu
595 600 605
tcc ctg cgc cac gtc gcc cgc acc cag gac aac gcg aag gcc gcc gtc 2172
Ser Leu Arg His Val Ala Arg Thr Gln Asp Asn Ala Lys Ala Ala Val
610 615 620
ctg gcc gac gcc ctg gac aag gcc acc gag aag gtc ctg tcc gag ggc 2220
Leu Ala Asp Ala Leu Asp Lys Ala Thr Glu Lys Val Leu Ser Glu Gly
625 630 635 640
aag tcc ccg tcc cgc aag gtc ggc gag atc gac aac cgt ggc tcc cac 2268
Lys Ser Pro Ser Arg Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His
645 650 655
ttc tac ctc gcg tcc tac tgg gcc gag gcc ctg gcc gca cag tcc gac 2316
Phe Tyr Leu Ala Ser Tyr Trp Ala Glu Ala Leu Ala Ala Gln Ser Asp
660 665 670
gac gcc gag ctg gcc gcg acc ttc cag ccg gtc gcc gag gcc ctg gcc 2364
Asp Ala Glu Leu Ala Ala Thr Phe Gln Pro Val Ala Glu Ala Leu Ala
675 680 685
gcc gac gcc gcg acc atc gac cag gag ctc atc gac aac cag ggt tcc 2412
Ala Asp Ala Ala Thr Ile Asp Gln Glu Leu Ile Asp Asn Gln Gly Ser
690 695 700
gcc gtg gac ctc ggc ggc tac tac gcc ccg tcc gat gag aag acc tcc 2460
Ala Val Asp Leu Gly Gly Tyr Tyr Ala Pro Ser Asp Glu Lys Thr Ser
705 710 715 720
gac gtg atg cgt ccg tcc gcg aag ttc aac gag atc atc gac ggt ctg 2508
Asp Val Met Arg Pro Ser Ala Lys Phe Asn Glu Ile Ile Asp Gly Leu
725 730 735
aag aag taa cccccgcttt ccgacgccgc cctgccccgc caccggggca 2557
Lys Lys
gggcggcgtc gcctttgggc agggccccgg acgaaccact tggtctaccg tccctttatt 2617
tttattcatg cgccctgaac tgcggagaca gggtttggac agacagctcg gtttacgtcg 2677
aaaagcggtg cgccgtactc ttctcccatg acccgatacg acaactccgc agcagaccag 2737
tgggacttcg agacccgctc catccacgcc ggccagaacc cggaccccgc gaccggttcc 2797
cgtaaccagc cgatccacct 2817
<210> 132
<211> 738
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 132
Met Ala Lys Ile Ile Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala
1 5 10 15
Thr Tyr Ser Phe Lys Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly
20 25 30
Ile Asp Val Glu Thr Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala
35 40 45
Gln Phe Pro Asp Arg Leu Thr Glu Glu Gln Arg Val Ala Asp Ala Leu
50 55 60
Thr Glu Leu Gly Asp Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys
65 70 75 80
Leu Pro Asn Ile Ser Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys
85 90 95
Glu Leu Gln Asp Gln Gly Tyr Asp Leu Pro Asp Tyr Val Asp Ser Pro
100 105 110
Glu Thr Asp Glu Glu Lys Asp Ala Arg Lys Arg Tyr Asp Val Val Lys
115 120 125
Gly Ser Ala Val Asn Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg
130 135 140
Ala Pro Glu Ala Val Lys Asn Phe Val Arg Lys Asn Pro His Arg Met
145 150 155 160
Gly Glu Trp Ser Ala Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ser
165 170 175
Asp Asp Phe Arg His Asn Glu Gln Ser Val Ile Leu Pro Ala Glu Asp
180 185 190
Thr Leu Ser Phe Val His Val Ala Thr Asp Gly Thr Glu Thr Lys Leu
195 200 205
Arg Asp Asp Leu Lys Val Leu Glu Gly Glu Val Val Asp Gly Thr Val
210 215 220
Met Arg Ala Ala Ala Leu Gln Glu Phe Leu Ala Glu Gln Val Lys Arg
225 230 235 240
Ala Gln Asp Glu Gly Ile Leu Phe Ser Val His Leu Lys Ala Thr Met
245 250 255
Met Lys Val Ser Asp Pro Ile Ile Phe Gly Tyr Val Val Arg Ala Phe
260 265 270
Phe Ala Asp Val Tyr Glu Lys Tyr Gly Glu Glu Leu Leu Ala Ala Gly
275 280 285
Leu Asp Gly Glu Asn Gly Leu Gly Ala Ile Phe Ser Gly Leu Glu Glu
290 295 300
Leu Glu Asn Gly Glu Glu Ile Arg Arg Ala Phe Asp Glu Ala Leu Glu
305 310 315 320
Asn Gly Pro Asp Leu Ala Met Val Asn Ser Asp Lys Gly Ile Thr Asn
325 330 335
Leu His Val Pro Ser Asp Val Ile Val Asp Ala Ser Met Pro Ala Met
340 345 350
Ile Arg Thr Ser Gly Gln Met Trp Asn Lys Asp Asp Lys Thr Gln Asp
355 360 365
Thr Leu Ala Val Ile Pro Asp Ser Ser Tyr Ala Gly Val Phe Gln Thr
370 375 380
Val Ile Asp Asp Cys Lys Ala Asn Gly Ala Phe Asp Pro Thr Thr Met
385 390 395 400
Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr
405 410 415
Gly Ser His Asp Lys Thr Phe Arg Ile Ala Ala Asp Gly Lys Val Glu
420 425 430
Val Arg Asn Ser Ala Gly Asp Val Leu Leu Glu His Glu Val Ser Glu
435 440 445
Gly Asp Ile Trp Arg Ala Cys Gln Thr Lys Asp Ala Pro Ile Gln Asp
450 455 460
Trp Val Lys Leu Ala Val Asp Arg Ala Arg Ile Ser Gly Met Pro Ala
465 470 475 480
Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp Arg Asn Leu Thr Thr
485 490 495
Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile
500 505 510
Arg Ile Leu Ser Pro Val Glu Ala Thr Gln Leu Ser Val Asp Arg Ile
515 520 525
Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp
530 535 540
Tyr Asn Thr Asp Leu Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys
545 550 555 560
Met Leu Ser Val Val Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr
565 570 575
Gly Ala Gly Gly Ser Ala Pro Lys His Val Gln Gln Val Val Glu Glu
580 585 590
Asn His Leu Arg Trp Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu
595 600 605
Ser Leu Arg His Val Ala Arg Thr Gln Asp Asn Ala Lys Ala Ala Val
610 615 620
Leu Ala Asp Ala Leu Asp Lys Ala Thr Glu Lys Val Leu Ser Glu Gly
625 630 635 640
Lys Ser Pro Ser Arg Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His
645 650 655
Phe Tyr Leu Ala Ser Tyr Trp Ala Glu Ala Leu Ala Ala Gln Ser Asp
660 665 670
Asp Ala Glu Leu Ala Ala Thr Phe Gln Pro Val Ala Glu Ala Leu Ala
675 680 685
Ala Asp Ala Ala Thr Ile Asp Gln Glu Leu Ile Asp Asn Gln Gly Ser
690 695 700
Ala Val Asp Leu Gly Gly Tyr Tyr Ala Pro Ser Asp Glu Lys Thr Ser
705 710 715 720
Asp Val Met Arg Pro Ser Ala Lys Phe Asn Glu Ile Ile Asp Gly Leu
725 730 735
Lys Lys
<210> 133
<211> 3378
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(3078)
<223> ген аконитатгидразы
<400> 133
aggtcatcgg tgtcgatgcc ggcgggaatc atcgtggtgc ggcttctttc gcatggggcg 60
gcgtgcggac accgctattt ctatcctccg cgggtgtggc gggggtggcg acgcgccgga 120
cacacagcga aaagccacaa acaagacgct cgtactgtta gaattgcagc ggtcgttggt 180
ccgaatccct acacttgggg acgtaccacc gcgttctgcc ggtgcacgcc taccttgacc 240
ggggtagacg ggtgcgggta acgaacattc cagttcaatg aagaggaaat ggagctccct 300
gtg acc gaa agc aag aac tcc ttc aac gcc aag cgc acc ctc gag gtc 348
Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Arg Thr Leu Glu Val
1 5 10 15
ggc gag aag tcc tac gac tac ttc gcc ctc gac gcc gtg ccg ggc atg 396
Gly Glu Lys Ser Tyr Asp Tyr Phe Ala Leu Asp Ala Val Pro Gly Met
20 25 30
gag aag ctg ccg tac tcc ctg aag gtt ctc ggc gag aac ctt ctg cgt 444
Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg
35 40 45
acc gag gac ggc gca aac atc acc acc gag cac atc gag gca atc gcc 492
Thr Glu Asp Gly Ala Asn Ile Thr Thr Glu His Ile Glu Ala Ile Ala
50 55 60
aac tgg gat ccg aag gct gag ccc tcc atc gag atc cag ttc acc ccg 540
Asn Trp Asp Pro Lys Ala Glu Pro Ser Ile Glu Ile Gln Phe Thr Pro
65 70 75 80
gca cgt gtc ctc atg cag gac ttc acc ggt gtc gcc tgt gca gtc gac 588
Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Ala Cys Ala Val Asp
85 90 95
ctg gcc acc atg cgc gag gcc gtg aag acc ctg ggc ggc gac ccg gac 636
Leu Ala Thr Met Arg Glu Ala Val Lys Thr Leu Gly Gly Asp Pro Asp
100 105 110
gac gtc aac ccg ctg aac ccg gcc gag atg gtc atc gac cac tcc gtc 684
Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val
115 120 125
atc gtc gag gca ttc ggt acc tcc gcc gct ctg gag aag aac gtc gag 732
Ile Val Glu Ala Phe Gly Thr Ser Ala Ala Leu Glu Lys Asn Val Glu
130 135 140
atc gag tac cag cgc aac gag gag cgc tac cag ttc ctc cgc tgg ggt 780
Ile Glu Tyr Gln Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly
145 150 155 160
gcc gag aac ttc tcc aac ttc cgc gtc gtt ccc ccg gga acc ggc atc 828
Ala Glu Asn Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile
165 170 175
gtc cac cag gtc aac atc gag tac ctg tcc cgc gtt gtc ttc gac aac 876
Val His Gln Val Asn Ile Glu Tyr Leu Ser Arg Val Val Phe Asp Asn
180 185 190
aac ggc ctc gcc tac ccg gac acc tgc atc ggc acc gac tcc cac acc 924
Asn Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr
195 200 205
acc atg gag aac ggc ctg gga atc ctg ggc tgg ggc gtc ggc ggc atc 972
Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile
210 215 220
gag gcc gag gct gcc atg ctg ggc cag ccg gtc tcc atg ctc atc ccg 1020
Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro
225 230 235 240
cgc gtc gtc ggc ttc aag ctg acc ggt gag atc ccg gcc ggc gtc acc 1068
Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Ala Gly Val Thr
245 250 255
gcc acc gac gtg gtc ctc acc atc acc gag atg ctg cgt aac cac ggc 1116
Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asn His Gly
260 265 270
gtc gtc cag aag ttc gtc gag ttc tac ggc aac ggc gtc aag tcc gtc 1164
Val Val Gln Lys Phe Val Glu Phe Tyr Gly Asn Gly Val Lys Ser Val
275 280 285
ccg ctg gcg aac cgc gcc acc atc ggc aac atg tcc ccg gag ttc ggc 1212
Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly
290 295 300
tcc acc tgt gcc atg ttc ccg atc gac gag gag acc atc aag tac ctc 1260
Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Ile Lys Tyr Leu
305 310 315 320
gag ctc acc ggc cgc tcc gag gag gac atc gcc cgc gtc gag gca tac 1308
Glu Leu Thr Gly Arg Ser Glu Glu Asp Ile Ala Arg Val Glu Ala Tyr
325 330 335
gcc aag gct cag ggc atg tgg ctc tac gag gac gcc ccg gag gcc gag 1356
Ala Lys Ala Gln Gly Met Trp Leu Tyr Glu Asp Ala Pro Glu Ala Glu
340 345 350
tac tcc gag tac ctc gag ctg gac ctc tcc acc gtc gtc ccg tcc atc 1404
Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile
355 360 365
gcc ggc ccg aag cgc ccg cag gac cgc atc ctc ctc tcc gag gcc aag 1452
Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys
370 375 380
gca cag ttc cgc aag gac ctg ccg acc tac gcc tcc gac gag gtc gct 1500
Ala Gln Phe Arg Lys Asp Leu Pro Thr Tyr Ala Ser Asp Glu Val Ala
385 390 395 400
gag ccg gtc cgc gcc acc aag gcc gtc ggc gac gac tac aac cag tcc 1548
Glu Pro Val Arg Ala Thr Lys Ala Val Gly Asp Asp Tyr Asn Gln Ser
405 410 415
ttc ccg ggc aac ggc gag tcc gct gcc gac tcc gcc gag ggc cgc gcc 1596
Phe Pro Gly Asn Gly Glu Ser Ala Ala Asp Ser Ala Glu Gly Arg Ala
420 425 430
tcc aag ccg gtc atc gtc gag tcc ccg cag ggc ggc gag tac acc ctc 1644
Ser Lys Pro Val Ile Val Glu Ser Pro Gln Gly Gly Glu Tyr Thr Leu
435 440 445
gac cac ggc atg gtg gcc atc gcc tcc atc acc tcc tgc acc aac acc 1692
Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr
450 455 460
tcc aac ccg tcc gtg ctg gtc gca gcc ggc ctg ctg gcc cgc aag gcc 1740
Ser Asn Pro Ser Val Leu Val Ala Ala Gly Leu Leu Ala Arg Lys Ala
465 470 475 480
aac gag ctg ggc ctg aag tcc aag ccg tgg gtc aag acc atc tgc gcc 1788
Asn Glu Leu Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala
485 490 495
ccg ggc tcc cag gtc gtc gac ggt tac ttc aag cgc gcc gac ctc tgg 1836
Pro Gly Ser Gln Val Val Asp Gly Tyr Phe Lys Arg Ala Asp Leu Trp
500 505 510
aag gat ctg gag gcc ctg ggc ttc tac ctc tcc ggc ttc ggc tgc acc 1884
Lys Asp Leu Glu Ala Leu Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr
515 520 525
tcc tgc atc ggc aac tcc ggt ccg ctc ccg gct gag gtc tcc tcc gcc 1932
Ser Cys Ile Gly Asn Ser Gly Pro Leu Pro Ala Glu Val Ser Ser Ala
530 535 540
atc aac gag cac gac ctg gcc gcc acc gca gtc ctg tcc ggc aac cgt 1980
Ile Asn Glu His Asp Leu Ala Ala Thr Ala Val Leu Ser Gly Asn Arg
545 550 555 560
aac ttc gag ggt cgt atc tcc ccc gac gtc aag atg aac tac ctg gcc 2028
Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala
565 570 575
tcc ccg ccg ctg gtc atc gcc tac gcc atc gcc ggc acc atg gac ttc 2076
Ser Pro Pro Leu Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe
580 585 590
gac ttc gag acc gat gcc ctg ggt cag gac aag gac ggc aac gac gtc 2124
Asp Phe Glu Thr Asp Ala Leu Gly Gln Asp Lys Asp Gly Asn Asp Val
595 600 605
ttc ctg aag gac atc tgg ccc tcc acc gag gag atc gag gag acc atc 2172
Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Glu Thr Ile
610 615 620
gcg gcc tcc atc tcc cgc gag ctg tac gag gcc gac tac gct gac gtc 2220
Ala Ala Ser Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val
625 630 635 640
ttc aag ggc gac aag cag tgg cag gag ctc gac atc ccg acc ggc aag 2268
Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Ile Pro Thr Gly Lys
645 650 655
acc ttc gag tgg gac gag aac tcc tcc tac atc cgt aag gca ccg tac 2316
Thr Phe Glu Trp Asp Glu Asn Ser Ser Tyr Ile Arg Lys Ala Pro Tyr
660 665 670
ttc gac ggc atg ccg ctc gag ccg cag ccg gtc gag gac atc aag ggc 2364
Phe Asp Gly Met Pro Leu Glu Pro Gln Pro Val Glu Asp Ile Lys Gly
675 680 685
gcc cgc gtc ctc gcc aag ctc ggc gac tcc gtg acc acc gac cac atc 2412
Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile
690 695 700
tcc ccg gcc tcc tcc atc aag ccg ggc acc ccg gca gcc cag tac ctg 2460
Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu
705 710 715 720
gac tcc atg ggt gtc gag cgc aag gac tac aac tcc ctg ggc tcc cgt 2508
Asp Ser Met Gly Val Glu Arg Lys Asp Tyr Asn Ser Leu Gly Ser Arg
725 730 735
cgt ggt aac cac gag gtc atg gtc cgc ggt acc ttc gcg aac atc cgc 2556
Arg Gly Asn His Glu Val Met Val Arg Gly Thr Phe Ala Asn Ile Arg
740 745 750
ctg gcc aac cag ctg gtc gac gtc acc ggt ggc tac acc cgt gac ttc 2604
Leu Ala Asn Gln Leu Val Asp Val Thr Gly Gly Tyr Thr Arg Asp Phe
755 760 765
acc cag gag ggt ggc ccg cag gcc tac atc tac gac gcc gcc atg aac 2652
Thr Gln Glu Gly Gly Pro Gln Ala Tyr Ile Tyr Asp Ala Ala Met Asn
770 775 780
tac aag gag gcc ggc acc ccg ctg gtc gtc ctg ggt ggc aag gag tac 2700
Tyr Lys Glu Ala Gly Thr Pro Leu Val Val Leu Gly Gly Lys Glu Tyr
785 790 795 800
ggc acc ggt tcc tcc cgt gac tgg gcc gcg aag ggc acc atc ctg ctc 2748
Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Ile Leu Leu
805 810 815
ggc gtg aag gcc gtc atc acc gag tcc ttc gag cgt atc cac cgc tcg 2796
Gly Val Lys Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser
820 825 830
aac ctg atc ggt atg ggt gtc atc ccg ctg cag ttc ccg gag ggc gag 2844
Asn Leu Ile Gly Met Gly Val Ile Pro Leu Gln Phe Pro Glu Gly Glu
835 840 845
tcc cac gag tcc ctg ggc ctg gac ggc acc gag gtc ttc gac atc gag 2892
Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Val Phe Asp Ile Glu
850 855 860
ggc atc acc gcc ctg aac gag ggc gag acc ccg aag acc gtc aag gtc 2940
Gly Ile Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val
865 870 875 880
acc gcc acc aag gag gac ggc tcc acc gtg gag ttc gac gcg att gtc 2988
Thr Ala Thr Lys Glu Asp Gly Ser Thr Val Glu Phe Asp Ala Ile Val
885 890 895
cgc atc gac acc ccg ggt gag gcc gac tac tac cgc aac ggc ggc atc 3036
Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg Asn Gly Gly Ile
900 905 910
ctg cag tac gtc ctg cgt cag atg atc gct gct gcc aag taa 3078
Leu Gln Tyr Val Leu Arg Gln Met Ile Ala Ala Ala Lys
915 920 925
gcgagcatcc tcccgcagtg tgaactgcct ctgagagggg gctgcatccc cggctaaggc 3138
gtcacgccac cggggagagc agccccctct cccctttttc cctattcccc cgacaggagt 3198
cccctcatgc ccgtggtcag cgacaccgag ttgacgcgtc gtcagaccga gatcctcgaa 3258
ggcgcgcggc gttgcttcgc cgagtacggc tacgagggcg ccacggtgcg ccgcctcgag 3318
gaggccacgg gcaagtcgcg gggtgcgatc ttccaccact tcggtgacaa ggagaacctc 3378
<210> 134
<211> 925
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 134
Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Arg Thr Leu Glu Val
1 5 10 15
Gly Glu Lys Ser Tyr Asp Tyr Phe Ala Leu Asp Ala Val Pro Gly Met
20 25 30
Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg
35 40 45
Thr Glu Asp Gly Ala Asn Ile Thr Thr Glu His Ile Glu Ala Ile Ala
50 55 60
Asn Trp Asp Pro Lys Ala Glu Pro Ser Ile Glu Ile Gln Phe Thr Pro
65 70 75 80
Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Ala Cys Ala Val Asp
85 90 95
Leu Ala Thr Met Arg Glu Ala Val Lys Thr Leu Gly Gly Asp Pro Asp
100 105 110
Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val
115 120 125
Ile Val Glu Ala Phe Gly Thr Ser Ala Ala Leu Glu Lys Asn Val Glu
130 135 140
Ile Glu Tyr Gln Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly
145 150 155 160
Ala Glu Asn Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile
165 170 175
Val His Gln Val Asn Ile Glu Tyr Leu Ser Arg Val Val Phe Asp Asn
180 185 190
Asn Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr
195 200 205
Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile
210 215 220
Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro
225 230 235 240
Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Ala Gly Val Thr
245 250 255
Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asn His Gly
260 265 270
Val Val Gln Lys Phe Val Glu Phe Tyr Gly Asn Gly Val Lys Ser Val
275 280 285
Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly
290 295 300
Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Ile Lys Tyr Leu
305 310 315 320
Glu Leu Thr Gly Arg Ser Glu Glu Asp Ile Ala Arg Val Glu Ala Tyr
325 330 335
Ala Lys Ala Gln Gly Met Trp Leu Tyr Glu Asp Ala Pro Glu Ala Glu
340 345 350
Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile
355 360 365
Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys
370 375 380
Ala Gln Phe Arg Lys Asp Leu Pro Thr Tyr Ala Ser Asp Glu Val Ala
385 390 395 400
Glu Pro Val Arg Ala Thr Lys Ala Val Gly Asp Asp Tyr Asn Gln Ser
405 410 415
Phe Pro Gly Asn Gly Glu Ser Ala Ala Asp Ser Ala Glu Gly Arg Ala
420 425 430
Ser Lys Pro Val Ile Val Glu Ser Pro Gln Gly Gly Glu Tyr Thr Leu
435 440 445
Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr
450 455 460
Ser Asn Pro Ser Val Leu Val Ala Ala Gly Leu Leu Ala Arg Lys Ala
465 470 475 480
Asn Glu Leu Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala
485 490 495
Pro Gly Ser Gln Val Val Asp Gly Tyr Phe Lys Arg Ala Asp Leu Trp
500 505 510
Lys Asp Leu Glu Ala Leu Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr
515 520 525
Ser Cys Ile Gly Asn Ser Gly Pro Leu Pro Ala Glu Val Ser Ser Ala
530 535 540
Ile Asn Glu His Asp Leu Ala Ala Thr Ala Val Leu Ser Gly Asn Arg
545 550 555 560
Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala
565 570 575
Ser Pro Pro Leu Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe
580 585 590
Asp Phe Glu Thr Asp Ala Leu Gly Gln Asp Lys Asp Gly Asn Asp Val
595 600 605
Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Glu Thr Ile
610 615 620
Ala Ala Ser Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val
625 630 635 640
Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Ile Pro Thr Gly Lys
645 650 655
Thr Phe Glu Trp Asp Glu Asn Ser Ser Tyr Ile Arg Lys Ala Pro Tyr
660 665 670
Phe Asp Gly Met Pro Leu Glu Pro Gln Pro Val Glu Asp Ile Lys Gly
675 680 685
Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile
690 695 700
Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu
705 710 715 720
Asp Ser Met Gly Val Glu Arg Lys Asp Tyr Asn Ser Leu Gly Ser Arg
725 730 735
Arg Gly Asn His Glu Val Met Val Arg Gly Thr Phe Ala Asn Ile Arg
740 745 750
Leu Ala Asn Gln Leu Val Asp Val Thr Gly Gly Tyr Thr Arg Asp Phe
755 760 765
Thr Gln Glu Gly Gly Pro Gln Ala Tyr Ile Tyr Asp Ala Ala Met Asn
770 775 780
Tyr Lys Glu Ala Gly Thr Pro Leu Val Val Leu Gly Gly Lys Glu Tyr
785 790 795 800
Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Ile Leu Leu
805 810 815
Gly Val Lys Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser
820 825 830
Asn Leu Ile Gly Met Gly Val Ile Pro Leu Gln Phe Pro Glu Gly Glu
835 840 845
Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Val Phe Asp Ile Glu
850 855 860
Gly Ile Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val
865 870 875 880
Thr Ala Thr Lys Glu Asp Gly Ser Thr Val Glu Phe Asp Ala Ile Val
885 890 895
Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg Asn Gly Gly Ile
900 905 910
Leu Gln Tyr Val Leu Arg Gln Met Ile Ala Ala Ala Lys
915 920 925
<210> 135
<211> 1752
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1452)
<223> ген цитратсинтазы
<400> 135
agctgctctc cgcccagcag ctcgaggagc tcggcttcaa cgccgtcatc tacccggtga 60
ccaccctgcg catcgccatg ggacaggtcg aggaggctct cgcggagatc gccgccaccg 120
gcacccagac cgggtgggtc gaccgcatgc agcaccgctc ccgcctctac gaactgctcc 180
gctacaacga gtacaacgcc ttcgaccagg aggttttcac gtactccgcc gacacctacc 240
agcccacctt ccagtagccc accagcaact cctgacattc cttgaaaggg gatgagtacc 300
atg tcc gac aac acc aac atc gag gtc cgc aag ggt ctc tac ggc gtg 348
Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val
1 5 10 15
atc gcc gac tac acc gca gtc tcc aag gtc atg ccg gag acc aac tcc 396
Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser
20 25 30
ctg acc tac cgc gga tac gcc gtc cag gac ctc gtc gca gag tgc tcc 444
Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser
35 40 45
ttc gag gag gtc ttc tac ctc ctg tgg aac ggt gag ctg ccg aac gag 492
Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu
50 55 60
gag cag ctg gcg gag ttc aac agg cgc ggc cgt tcc tac cgc aag ctc 540
Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu
65 70 75 80
gac gcc ggc ctg atc gca ctc atc cac tcc ctg ccg ctc gac tgt cac 588
Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His
85 90 95
ccg atg gac gtc atg cgc acc gcc gtg tcc tac atg ggc acc aag gac 636
Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp
100 105 110
tcc gag cac ttc acc ccg gac gcc gac cac atc acc cac gtc ggc cac 684
Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His
115 120 125
aac ctc ctc gcc cag ctg ccg atg gtg ctg gcc atg gac atc cgc cgc 732
Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg
130 135 140
cgt cag ggc ctc gac atc gtc gcc ccg gac ccg gac aag tcc gtc gcc 780
Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala
145 150 155 160
cac aac ctg ctg tcg atg gtc ttc ggc aac ggc ccg gag tcc ccg gcg 828
His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala
165 170 175
tcc aac ccg gac gac gtg cgt gac ttc gag aag tcg ctc atc ctc tac 876
Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr
180 185 190
gcc gag cac tcc ttc aac gcc tcg acc ttc acc tcg cgt gtg atc acc 924
Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr
195 200 205
tcg acc cgc tcg gac gtc tac tcc gcg atc acc ggt gcc atc ggc gcc 972
Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala
210 215 220
ctc aag ggc ccg ctg cac ggc ggc gcc aac gag ttc gtc atg cac acc 1020
Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr
225 230 235 240
atg ctg gag atc gac gac ccg gag aag gcc gcc gac tgg gtc aac aac 1068
Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn
245 250 255
gcc ctg gac aac aag gac ctc atc atg ggc ttc ggt cac cgc gtg tac 1116
Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr
260 265 270
aag aag ggt gac tcg cgt gtc ccg tcc atg gag gcg tcc ttc cgt gac 1164
Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp
275 280 285
ctc gcc gag cgt cac gac ggc gcc aag tgg gtc gcc atg tac gag aac 1212
Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn
290 295 300
atg cgc gac gcc atg gac gcc cgc acc ggc atc aag ccg aac ctg gac 1260
Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp
305 310 315 320
ttc ccg gcc ggc ccg gcg tac cac ctg ctc ggc ttc ccg gtc gac ttc 1308
Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe
325 330 335
ttc acc ccg ctg ttc gtc gtc gcc cgc atc gcc ggc tgg acc gcc cac 1356
Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His
340 345 350
atc gtg gag cag tac aac gac aac tcc ctg att cgt ccg ctc tcc gcc 1404
Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala
355 360 365
tac aac ggc ccc gag cag cgc cag gtc acc ccg ctg gcc gcc cgt tag 1452
Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg
370 375 380
ccccacggcc ggcaccggac caccgcgacg cccacggcac tgcccacaca ttggggtggt 1512
gcgcgtgggt ttcgctgttc ggaacacctg tcggcccatt ccgcagggcg ggacagaaac 1572
ggcctttcgg gcgcccgtcc cgcggagtgc ggaaatggac cgcaccgggt ccaccacctg 1632
cagatacccc ctgtgactag gggatactcc cccgaagaca accctctttc ttgcgatact 1692
gtggtgtgcc agagagctca cccgctctca gacacacccc cgacatgacg ttcggaaagg 1752
<210> 136
<211> 383
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 136
Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val
1 5 10 15
Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser
20 25 30
Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser
35 40 45
Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu
50 55 60
Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu
65 70 75 80
Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His
85 90 95
Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp
100 105 110
Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His
115 120 125
Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg
130 135 140
Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala
145 150 155 160
His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala
165 170 175
Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr
180 185 190
Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr
195 200 205
Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala
210 215 220
Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr
225 230 235 240
Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn
245 250 255
Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr
260 265 270
Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp
275 280 285
Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn
290 295 300
Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp
305 310 315 320
Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe
325 330 335
Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His
340 345 350
Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala
355 360 365
Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg
370 375 380
<210> 137
<211> 3372
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(3072)
<223> ген pepc
<400> 137
cggttccgtc aagaccgact cggtcgccga gatcgtcggt cagcccgacg tcgacggtgg 60
tctcgtcggt ggcgcctcgc tcgacggcga ggagttcgcc aagctggcgg ccgccgcggc 120
gtccgtcgtc tagcatcacc tcatccaccg gccccgggca ctgaacattc agtgcccggg 180
gccggtgtcc tttctccggg gaatatggtg cgcaatcccc acgcacggcc gtcatatgcg 240
tcatgatggg gaggcgaacc gcatccccgc cttcttcgag tacgaacagg gagacactca 300
atg acc gtc cgc gat cat ctg cag gaa gac atc cgc tac ctc ggc cgt 348
Met Thr Val Arg Asp His Leu Gln Glu Asp Ile Arg Tyr Leu Gly Arg
1 5 10 15
atc ctc ggc cag gtc atc gcg gaa cag gag ggc gag gac gtg ttc aac 396
Ile Leu Gly Gln Val Ile Ala Glu Gln Glu Gly Glu Asp Val Phe Asn
20 25 30
ctc gtc gag cac gcc cgc cag cag gcc ttc gag gtg gcc aag ggc aac 444
Leu Val Glu His Ala Arg Gln Gln Ala Phe Glu Val Ala Lys Gly Asn
35 40 45
gcc tcc ctg gag gtg ctc gtc gac ctg ttc cgg aac atc gag ccg gag 492
Ala Ser Leu Glu Val Leu Val Asp Leu Phe Arg Asn Ile Glu Pro Glu
50 55 60
cgc gcg acc ccg gtg atc cgg gcg ttc agc cac ttc gcc ctg atg gcg 540
Arg Ala Thr Pro Val Ile Arg Ala Phe Ser His Phe Ala Leu Met Ala
65 70 75 80
aac ctc gcg gag gac atc cac gac gac ttc cac cgg gag cgc atc ctc 588
Asn Leu Ala Glu Asp Ile His Asp Asp Phe His Arg Glu Arg Ile Leu
85 90 95
gat gag ggc ggg gcc ccc gac tcc acc ctc aac gcc acg tgg gag aag 636
Asp Glu Gly Gly Ala Pro Asp Ser Thr Leu Asn Ala Thr Trp Glu Lys
100 105 110
ttc cgg gag gcc ggc gtc agt gcc gcg gac atc gag agg tcg ctg tct 684
Phe Arg Glu Ala Gly Val Ser Ala Ala Asp Ile Glu Arg Ser Leu Ser
115 120 125
gcc ggg ctc gtc gcc ccg gtg ctc acc gcc cac ccg acg gag acc cgc 732
Ala Gly Leu Val Ala Pro Val Leu Thr Ala His Pro Thr Glu Thr Arg
130 135 140
cgc cgc acc gtc ttc gac gcc cag aag cac atc acc gag ctc atg ctg 780
Arg Arg Thr Val Phe Asp Ala Gln Lys His Ile Thr Glu Leu Met Leu
145 150 155 160
cag cgg cac gcc gtc cag gac gcg gag ccg aac gcc cgc acc gag gac 828
Gln Arg His Ala Val Gln Asp Ala Glu Pro Asn Ala Arg Thr Glu Asp
165 170 175
cgt ctc gcg gag atc gag cgc aac atc cgc cgc cgc atg acc atc ctg 876
Arg Leu Ala Glu Ile Glu Arg Asn Ile Arg Arg Arg Met Thr Ile Leu
180 185 190
tgg cag acc gcg ctc atc cgg cag gcc cgc ccc cgc atc gag gac gag 924
Trp Gln Thr Ala Leu Ile Arg Gln Ala Arg Pro Arg Ile Glu Asp Glu
195 200 205
atc gag gtc ggc ctg cgc tac tac acg ctc tcc ctg ctc cgg gag atc 972
Ile Glu Val Gly Leu Arg Tyr Tyr Thr Leu Ser Leu Leu Arg Glu Ile
210 215 220
ccg gcg ctc aac cgg cac gtc gtc gac acg ctc acg gag cgt ttc ggc 1020
Pro Ala Leu Asn Arg His Val Val Asp Thr Leu Thr Glu Arg Phe Gly
225 230 235 240
gcc gac ctg cgc aac agc gcc ggt cag gcc atc gtc cgc ccg ggc tcc 1068
Ala Asp Leu Arg Asn Ser Ala Gly Gln Ala Ile Val Arg Pro Gly Ser
245 250 255
tgg atc ggc ggc gac cac gac ggc aac ccc ttc gtc acc gcc gac aca 1116
Trp Ile Gly Gly Asp His Asp Gly Asn Pro Phe Val Thr Ala Asp Thr
260 265 270
ctg gac tac gct tcc cga cgc gcc gcc cag acc gtc ctc aag tac tac 1164
Leu Asp Tyr Ala Ser Arg Arg Ala Ala Gln Thr Val Leu Lys Tyr Tyr
275 280 285
gtc acg cag ctc cac gcc ctc gag cac gag ctg agc ctc tcc gac cgc 1212
Val Thr Gln Leu His Ala Leu Glu His Glu Leu Ser Leu Ser Asp Arg
290 295 300
atg acc tcc gtc acc gtg gag ctc gtc gcc ctc gcc ggg cgg gga aag 1260
Met Thr Ser Val Thr Val Glu Leu Val Ala Leu Ala Gly Arg Gly Lys
305 310 315 320
aac gac gtc ccc tcg cgt gtc gac gag ccc tac cgc cgc gcc gtc cac 1308
Asn Asp Val Pro Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val His
325 330 335
ggc gtc cgg ggc cgc atc ctc gcc acg acc gcg gcg ctc atc ggg gag 1356
Gly Val Arg Gly Arg Ile Leu Ala Thr Thr Ala Ala Leu Ile Gly Glu
340 345 350
gac gcc gtc gag ggc gtc tgg cac cgc gag cat gag ccc tac ggc tcc 1404
Asp Ala Val Glu Gly Val Trp His Arg Glu His Glu Pro Tyr Gly Ser
355 360 365
ccg cag gag ttc gag gcg gac ctc agg gtc atc gac acc tcg ctg cgt 1452
Pro Gln Glu Phe Glu Ala Asp Leu Arg Val Ile Asp Thr Ser Leu Arg
370 375 380
gcc tcc cac gac gag atc atc gcc gac gac cgt ctc agc tcc atc cgg 1500
Ala Ser His Asp Glu Ile Ile Ala Asp Asp Arg Leu Ser Ser Ile Arg
385 390 395 400
gcg gcc gtc gcc tcc ttc ggg ttc cac ctg tac tcg atc gac ctg cga 1548
Ala Ala Val Ala Ser Phe Gly Phe His Leu Tyr Ser Ile Asp Leu Arg
405 410 415
cag aac tcc gag tcc ttc gag aac gtc ctc acc gag gtc ttc gcc acc 1596
Gln Asn Ser Glu Ser Phe Glu Asn Val Leu Thr Glu Val Phe Ala Thr
420 425 430
gcg cac gtc cac ccc aac tac gac acg ctg cgg gag gag gtg aag atc 1644
Ala His Val His Pro Asn Tyr Asp Thr Leu Arg Glu Glu Val Lys Ile
435 440 445
gag ctg ctc acc cgc gaa ctg cag acc ccg cgc ccc ctc gtc ccg cgc 1692
Glu Leu Leu Thr Arg Glu Leu Gln Thr Pro Arg Pro Leu Val Pro Arg
450 455 460
ggc tac cgg ggc ttc tcc gag ccg acc cag cgg gag ctc gac ctc atc 1740
Gly Tyr Arg Gly Phe Ser Glu Pro Thr Gln Arg Glu Leu Asp Leu Ile
465 470 475 480
tcc cag gcc gcg gac tcc gtc gcc cgc ttc ggc gag cag atg atc ccg 1788
Ser Gln Ala Ala Asp Ser Val Ala Arg Phe Gly Glu Gln Met Ile Pro
485 490 495
cac cag atc atc tcc atg gcg cag tcg gtc agc gac atc ctc gag ccg 1836
His Gln Ile Ile Ser Met Ala Gln Ser Val Ser Asp Ile Leu Glu Pro
500 505 510
atg gtg ctg ctc aag gag gtc ggc ctc atc cag gcc aac ggc cag ggc 1884
Met Val Leu Leu Lys Glu Val Gly Leu Ile Gln Ala Asn Gly Gln Gly
515 520 525
ccg acc ggc agc gtc gac atc atc ccg ctg ttc gag acg atc gac gac 1932
Pro Thr Gly Ser Val Asp Ile Ile Pro Leu Phe Glu Thr Ile Asp Asp
530 535 540
ctc cag gcc ggc gcc ggc atc ctc cgt gag ctg tgg gac ctg ccg atc 1980
Leu Gln Ala Gly Ala Gly Ile Leu Arg Glu Leu Trp Asp Leu Pro Ile
545 550 555 560
tac cgg gcg tac ctc gcc cag cgc ggc gac atc cag gag gtc atg ctc 2028
Tyr Arg Ala Tyr Leu Ala Gln Arg Gly Asp Ile Gln Glu Val Met Leu
565 570 575
ggc tac tcg gac tcc aac aag gac ggc ggc tac ttc gcc gcc aac tgg 2076
Gly Tyr Ser Asp Ser Asn Lys Asp Gly Gly Tyr Phe Ala Ala Asn Trp
580 585 590
gcg ctc tac gac gcc gag acc gac ctg gtg aag gtc ggc cgc gag tac 2124
Ala Leu Tyr Asp Ala Glu Thr Asp Leu Val Lys Val Gly Arg Glu Tyr
595 600 605
ggt gtg cgc ctg cga ctc ttc cac ggc cgc ggc ggc acc gtc ggc cgt 2172
Gly Val Arg Leu Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly Arg
610 615 620
ggt ggc ggc ccc tcc tac gac gcg ctc ctc gcc cag ccg cag ggg gcg 2220
Gly Gly Gly Pro Ser Tyr Asp Ala Leu Leu Ala Gln Pro Gln Gly Ala
625 630 635 640
gtc gac ggt tcc gtg cgc atc acc gag cag ggc gag atc atc tcg gcc 2268
Val Asp Gly Ser Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser Ala
645 650 655
aag tac ggc tcc ccg cgt acg gcc cgc cgc aac ctg gag gca ctg gtc 2316
Lys Tyr Gly Ser Pro Arg Thr Ala Arg Arg Asn Leu Glu Ala Leu Val
660 665 670
tcc gcg acg ctc gag gcc agc ctg ctc acc gtc gac gac ctc gcc gac 2364
Ser Ala Thr Leu Glu Ala Ser Leu Leu Thr Val Asp Asp Leu Ala Asp
675 680 685
cgg gcc cgc gcg acg cag ata atg agc gaa ctc gcg cag atc tcc cgc 2412
Arg Ala Arg Ala Thr Gln Ile Met Ser Glu Leu Ala Gln Ile Ser Arg
690 695 700
cgc aag tac tcc gag ctc gtc cac gag gac ccg ggg ttc atc ccg tac 2460
Arg Lys Tyr Ser Glu Leu Val His Glu Asp Pro Gly Phe Ile Pro Tyr
705 710 715 720
ttc acc cag tcg acc ccg ctc gac gag atc ggc tcc ctc aac atc ggc 2508
Phe Thr Gln Ser Thr Pro Leu Asp Glu Ile Gly Ser Leu Asn Ile Gly
725 730 735
tcc cgc ccg acc gcc cgc aag cag acc aag ggc gtc gac gat ctc cgc 2556
Ser Arg Pro Thr Ala Arg Lys Gln Thr Lys Gly Val Asp Asp Leu Arg
740 745 750
gcc atc ccg tgg gtg ctc gcc tgg tcc cag tcg cgt gtg ctg ctg ccg 2604
Ala Ile Pro Trp Val Leu Ala Trp Ser Gln Ser Arg Val Leu Leu Pro
755 760 765
ggc tgg ttc ggc gtc ggc acg gcc ctg gcc gag tgg atc ggg gag ggg 2652
Gly Trp Phe Gly Val Gly Thr Ala Leu Ala Glu Trp Ile Gly Glu Gly
770 775 780
gag gac agg gac gag cgc atc gcc gag ctg cgg gag ctc tac gag tcc 2700
Glu Asp Arg Asp Glu Arg Ile Ala Glu Leu Arg Glu Leu Tyr Glu Ser
785 790 795 800
tgg ccc ttc ttc acc tcg atc atg tcc aac atg gcg cag gtc atg tcg 2748
Trp Pro Phe Phe Thr Ser Ile Met Ser Asn Met Ala Gln Val Met Ser
805 810 815
aag gcg ggc atg gat ctc gcg gag ctc tac gcc cgg ctc atc gac gac 2796
Lys Ala Gly Met Asp Leu Ala Glu Leu Tyr Ala Arg Leu Ile Asp Asp
820 825 830
cgg gag gtc gcc gag cgc gtc cac ggt gtc atc acg gag gag ttc gag 2844
Arg Glu Val Ala Glu Arg Val His Gly Val Ile Thr Glu Glu Phe Glu
835 840 845
ctc acc cgc gag atg ttc agc acc gtc acc ggc agc gcc gag ctg ctc 2892
Leu Thr Arg Glu Met Phe Ser Thr Val Thr Gly Ser Ala Glu Leu Leu
850 855 860
gcc gac aac ccg gcg ctg gcc cgt tcg gtg cgc agg cgc ttc ccg tac 2940
Ala Asp Asn Pro Ala Leu Ala Arg Ser Val Arg Arg Arg Phe Pro Tyr
865 870 875 880
ctg ctg ccg ctc aac atc atc cag ctg gag ctg ctg cgc cgc cac cgt 2988
Leu Leu Pro Leu Asn Ile Ile Gln Leu Glu Leu Leu Arg Arg His Arg
885 890 895
gcc ggc gac tcc cga cgc gcc gtg tcc cgc ggc atc cag ctg acg atg 3036
Ala Gly Asp Ser Arg Arg Ala Val Ser Arg Gly Ile Gln Leu Thr Met
900 905 910
aac gga ctc gcc acc gca ctg cgc aac tcc ggg tag acctggtgta 3082
Asn Gly Leu Ala Thr Ala Leu Arg Asn Ser Gly
915 920
gactgagccg gagtttgtgc cgcccgattc ccgcaaagga ccttcatgac cctcgcactc 3142
cagatcatcc tcgtgctcac cgccgtgctc atgacggtgt tcgttctgct gcacaagggt 3202
aagggcggcg gcctgtccag cctcttcggt ggcggcatgc agtcgaacct ctcgggttcg 3262
acggtcgtcg agaagaacct cgaccgctac acgatcctca tggccgtcat ctggatcgcc 3322
tgcatcgtgg cgctgaacct catccaggct ttcgccggct aggttcaatg 3372
<210> 138
<211> 923
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 138
Met Thr Val Arg Asp His Leu Gln Glu Asp Ile Arg Tyr Leu Gly Arg
1 5 10 15
Ile Leu Gly Gln Val Ile Ala Glu Gln Glu Gly Glu Asp Val Phe Asn
20 25 30
Leu Val Glu His Ala Arg Gln Gln Ala Phe Glu Val Ala Lys Gly Asn
35 40 45
Ala Ser Leu Glu Val Leu Val Asp Leu Phe Arg Asn Ile Glu Pro Glu
50 55 60
Arg Ala Thr Pro Val Ile Arg Ala Phe Ser His Phe Ala Leu Met Ala
65 70 75 80
Asn Leu Ala Glu Asp Ile His Asp Asp Phe His Arg Glu Arg Ile Leu
85 90 95
Asp Glu Gly Gly Ala Pro Asp Ser Thr Leu Asn Ala Thr Trp Glu Lys
100 105 110
Phe Arg Glu Ala Gly Val Ser Ala Ala Asp Ile Glu Arg Ser Leu Ser
115 120 125
Ala Gly Leu Val Ala Pro Val Leu Thr Ala His Pro Thr Glu Thr Arg
130 135 140
Arg Arg Thr Val Phe Asp Ala Gln Lys His Ile Thr Glu Leu Met Leu
145 150 155 160
Gln Arg His Ala Val Gln Asp Ala Glu Pro Asn Ala Arg Thr Glu Asp
165 170 175
Arg Leu Ala Glu Ile Glu Arg Asn Ile Arg Arg Arg Met Thr Ile Leu
180 185 190
Trp Gln Thr Ala Leu Ile Arg Gln Ala Arg Pro Arg Ile Glu Asp Glu
195 200 205
Ile Glu Val Gly Leu Arg Tyr Tyr Thr Leu Ser Leu Leu Arg Glu Ile
210 215 220
Pro Ala Leu Asn Arg His Val Val Asp Thr Leu Thr Glu Arg Phe Gly
225 230 235 240
Ala Asp Leu Arg Asn Ser Ala Gly Gln Ala Ile Val Arg Pro Gly Ser
245 250 255
Trp Ile Gly Gly Asp His Asp Gly Asn Pro Phe Val Thr Ala Asp Thr
260 265 270
Leu Asp Tyr Ala Ser Arg Arg Ala Ala Gln Thr Val Leu Lys Tyr Tyr
275 280 285
Val Thr Gln Leu His Ala Leu Glu His Glu Leu Ser Leu Ser Asp Arg
290 295 300
Met Thr Ser Val Thr Val Glu Leu Val Ala Leu Ala Gly Arg Gly Lys
305 310 315 320
Asn Asp Val Pro Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val His
325 330 335
Gly Val Arg Gly Arg Ile Leu Ala Thr Thr Ala Ala Leu Ile Gly Glu
340 345 350
Asp Ala Val Glu Gly Val Trp His Arg Glu His Glu Pro Tyr Gly Ser
355 360 365
Pro Gln Glu Phe Glu Ala Asp Leu Arg Val Ile Asp Thr Ser Leu Arg
370 375 380
Ala Ser His Asp Glu Ile Ile Ala Asp Asp Arg Leu Ser Ser Ile Arg
385 390 395 400
Ala Ala Val Ala Ser Phe Gly Phe His Leu Tyr Ser Ile Asp Leu Arg
405 410 415
Gln Asn Ser Glu Ser Phe Glu Asn Val Leu Thr Glu Val Phe Ala Thr
420 425 430
Ala His Val His Pro Asn Tyr Asp Thr Leu Arg Glu Glu Val Lys Ile
435 440 445
Glu Leu Leu Thr Arg Glu Leu Gln Thr Pro Arg Pro Leu Val Pro Arg
450 455 460
Gly Tyr Arg Gly Phe Ser Glu Pro Thr Gln Arg Glu Leu Asp Leu Ile
465 470 475 480
Ser Gln Ala Ala Asp Ser Val Ala Arg Phe Gly Glu Gln Met Ile Pro
485 490 495
His Gln Ile Ile Ser Met Ala Gln Ser Val Ser Asp Ile Leu Glu Pro
500 505 510
Met Val Leu Leu Lys Glu Val Gly Leu Ile Gln Ala Asn Gly Gln Gly
515 520 525
Pro Thr Gly Ser Val Asp Ile Ile Pro Leu Phe Glu Thr Ile Asp Asp
530 535 540
Leu Gln Ala Gly Ala Gly Ile Leu Arg Glu Leu Trp Asp Leu Pro Ile
545 550 555 560
Tyr Arg Ala Tyr Leu Ala Gln Arg Gly Asp Ile Gln Glu Val Met Leu
565 570 575
Gly Tyr Ser Asp Ser Asn Lys Asp Gly Gly Tyr Phe Ala Ala Asn Trp
580 585 590
Ala Leu Tyr Asp Ala Glu Thr Asp Leu Val Lys Val Gly Arg Glu Tyr
595 600 605
Gly Val Arg Leu Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly Arg
610 615 620
Gly Gly Gly Pro Ser Tyr Asp Ala Leu Leu Ala Gln Pro Gln Gly Ala
625 630 635 640
Val Asp Gly Ser Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser Ala
645 650 655
Lys Tyr Gly Ser Pro Arg Thr Ala Arg Arg Asn Leu Glu Ala Leu Val
660 665 670
Ser Ala Thr Leu Glu Ala Ser Leu Leu Thr Val Asp Asp Leu Ala Asp
675 680 685
Arg Ala Arg Ala Thr Gln Ile Met Ser Glu Leu Ala Gln Ile Ser Arg
690 695 700
Arg Lys Tyr Ser Glu Leu Val His Glu Asp Pro Gly Phe Ile Pro Tyr
705 710 715 720
Phe Thr Gln Ser Thr Pro Leu Asp Glu Ile Gly Ser Leu Asn Ile Gly
725 730 735
Ser Arg Pro Thr Ala Arg Lys Gln Thr Lys Gly Val Asp Asp Leu Arg
740 745 750
Ala Ile Pro Trp Val Leu Ala Trp Ser Gln Ser Arg Val Leu Leu Pro
755 760 765
Gly Trp Phe Gly Val Gly Thr Ala Leu Ala Glu Trp Ile Gly Glu Gly
770 775 780
Glu Asp Arg Asp Glu Arg Ile Ala Glu Leu Arg Glu Leu Tyr Glu Ser
785 790 795 800
Trp Pro Phe Phe Thr Ser Ile Met Ser Asn Met Ala Gln Val Met Ser
805 810 815
Lys Ala Gly Met Asp Leu Ala Glu Leu Tyr Ala Arg Leu Ile Asp Asp
820 825 830
Arg Glu Val Ala Glu Arg Val His Gly Val Ile Thr Glu Glu Phe Glu
835 840 845
Leu Thr Arg Glu Met Phe Ser Thr Val Thr Gly Ser Ala Glu Leu Leu
850 855 860
Ala Asp Asn Pro Ala Leu Ala Arg Ser Val Arg Arg Arg Phe Pro Tyr
865 870 875 880
Leu Leu Pro Leu Asn Ile Ile Gln Leu Glu Leu Leu Arg Arg His Arg
885 890 895
Ala Gly Asp Ser Arg Arg Ala Val Ser Arg Gly Ile Gln Leu Thr Met
900 905 910
Asn Gly Leu Ala Thr Ala Leu Arg Asn Ser Gly
915 920
<210> 139
<211> 2346
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2046)
<223> ген пируватдегидрогеназы
<400> 139
cggtcgccga gcgtgcccgt gccgagcatg agcccggcca gcacgagggg gtaggcgttg 60
atgatccaca gcgcctgcac ctcggaggtg tggagctgtc tgcgcagctc ggggagcgcg 120
gtgaagagga tcgagttgtc caccccgacc atcagcagcc ccagggagat gatggcgagg 180
aaggcccacc gctgggccgg ggtggagtcg acgttgtcga ggtgcgtcac gcagcggaac 240
tgtaccgacc gttcgttaca gtttcaacgc ggggcatgaa tcctccgtac gctcgggacc 300
atg gca cgc acc tac gca cag cag ctc gtc gac gcc ctc gag agg cag 348
Met Ala Arg Thr Tyr Ala Gln Gln Leu Val Asp Ala Leu Glu Arg Gln
1 5 10 15
ggc gtg gaa cgg atc tac gga gtc gtc ggg gac agc ctc aac ccc atc 396
Gly Val Glu Arg Ile Tyr Gly Val Val Gly Asp Ser Leu Asn Pro Ile
20 25 30
gtc gac gcc gtc cgc acc tcc ccg atc gag tgg atc cac gtc cgc aac 444
Val Asp Ala Val Arg Thr Ser Pro Ile Glu Trp Ile His Val Arg Asn
35 40 45
gag gag gcc gcc gcc ttc gcc gcc ggc gcc gag tcc ctc ctc acc ggc 492
Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Leu Thr Gly
50 55 60
cgc ctc gcc gtg tgc gcg ggc tcc tgc ggc ccc ggc aac ctc cac ctc 540
Arg Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His Leu
65 70 75 80
atc cag ggt ctc tac gac gcc cac cgc aac ggc gcc cac gtc ctc gcc 588
Ile Gln Gly Leu Tyr Asp Ala His Arg Asn Gly Ala His Val Leu Ala
85 90 95
ctg gcc tcc cac atc ccc agc aac cag atc ggt acc tca tac ttc cag 636
Leu Ala Ser His Ile Pro Ser Asn Gln Ile Gly Thr Ser Tyr Phe Gln
100 105 110
gag acc cac ccc gaa cag ctc ttc gcg gaa tgt tcc ggt tac tgc gag 684
Glu Thr His Pro Glu Gln Leu Phe Ala Glu Cys Ser Gly Tyr Cys Glu
115 120 125
atg gtc aac tcc gcc gac cag ggc gcc cgc gtc ctg cac cac gcg atc 732
Met Val Asn Ser Ala Asp Gln Gly Ala Arg Val Leu His His Ala Ile
130 135 140
cag tcg acg atg gcc ggg cag ggc gtg tcc gtg ctg gtc atc ccg ggg 780
Gln Ser Thr Met Ala Gly Gln Gly Val Ser Val Leu Val Ile Pro Gly
145 150 155 160
gac atc gcc acg cag gac gcc ggc gac gac cgg ttc ctg cag tcc acc 828
Asp Ile Ala Thr Gln Asp Ala Gly Asp Asp Arg Phe Leu Gln Ser Thr
165 170 175
gtc gcc acc ggc cgc ccc gtg ctc cac ccc gac gcc cgc gag gcc gcc 876
Val Ala Thr Gly Arg Pro Val Leu His Pro Asp Ala Arg Glu Ala Ala
180 185 190
gcc ctg gtc gcg gcc atc aac gcc gcc gac acg gtg acc ctg ttc tgc 924
Ala Leu Val Ala Ala Ile Asn Ala Ala Asp Thr Val Thr Leu Phe Cys
195 200 205
ggc gca ggc gta cgc gac gcc cgc gag cag gtc ctc gcc ctg gcg gag 972
Gly Ala Gly Val Arg Asp Ala Arg Glu Gln Val Leu Ala Leu Ala Glu
210 215 220
aag atc aag gcc ccc atc ggc cac gcc ctc ggc ggc aag atg cac atc 1020
Lys Ile Lys Ala Pro Ile Gly His Ala Leu Gly Gly Lys Met His Ile
225 230 235 240
cag cac gac aac ccc ttc gac gtc ggc atg tcc ggg ctc ctc ggc tac 1068
Gln His Asp Asn Pro Phe Asp Val Gly Met Ser Gly Leu Leu Gly Tyr
245 250 255
ggc gcc tgc cac gac gca ctg cac gac gcc gac ctg ctc atc ctc ctg 1116
Gly Ala Cys His Asp Ala Leu His Asp Ala Asp Leu Leu Ile Leu Leu
260 265 270
ggc acc gac ttc ccc tac acc gag ttc ctg ccc acc ggg aac gtc gcg 1164
Gly Thr Asp Phe Pro Tyr Thr Glu Phe Leu Pro Thr Gly Asn Val Ala
275 280 285
cag gtc gac agc gac gcc gcg cac atc ggc cgc cgc acc acg gtg cac 1212
Gln Val Asp Ser Asp Ala Ala His Ile Gly Arg Arg Thr Thr Val His
290 295 300
cac ccg gtg gtc ggc gac gtc ggc gcc acc ctc gac acc atc ctc ccg 1260
His Pro Val Val Gly Asp Val Gly Ala Thr Leu Asp Thr Ile Leu Pro
305 310 315 320
cac ctg gag gag aag acc gac cgc agc ttc ctc gac ggg atg ctc cgc 1308
His Leu Glu Glu Lys Thr Asp Arg Ser Phe Leu Asp Gly Met Leu Arg
325 330 335
aag cac gcc cgg acg ctg gag aag gtc gtg gag gtg tac acg cgg gac 1356
Lys His Ala Arg Thr Leu Glu Lys Val Val Glu Val Tyr Thr Arg Asp
340 345 350
gtc ggg aag cac acc ccc atc cac ccg gag tac ctc gcc gcc gtc ctc 1404
Val Gly Lys His Thr Pro Ile His Pro Glu Tyr Leu Ala Ala Val Leu
355 360 365
gac gag ctc gcc gcc gac gac gcc gtg ttc acc gtc gac acc ggc atg 1452
Asp Glu Leu Ala Ala Asp Asp Ala Val Phe Thr Val Asp Thr Gly Met
370 375 380
tgc aac gtg tgg gcc gcg cgc tac ctc acc ccc aac gga cga cgc gcg 1500
Cys Asn Val Trp Ala Ala Arg Tyr Leu Thr Pro Asn Gly Arg Arg Ala
385 390 395 400
cag atc ggc tcc ctc cgc cac ggc acg atg gcc aac gcg ctg ccg cac 1548
Gln Ile Gly Ser Leu Arg His Gly Thr Met Ala Asn Ala Leu Pro His
405 410 415
gcc atc ggc gcg cag ttc gcc gac cgg aac cgt cag gtc atc agc ctc 1596
Ala Ile Gly Ala Gln Phe Ala Asp Arg Asn Arg Gln Val Ile Ser Leu
420 425 430
aac ggc gac ggc ggc ctc gcc atg ctc ctc ggc gaa ctg ctc acc gtg 1644
Asn Gly Asp Gly Gly Leu Ala Met Leu Leu Gly Glu Leu Leu Thr Val
435 440 445
gaa ctc cac gac ctg ccc gtg aag atg gtc gtg ttc aac aac tcc tcg 1692
Glu Leu His Asp Leu Pro Val Lys Met Val Val Phe Asn Asn Ser Ser
450 455 460
ctc ggc atg gtc aaa ctc gag atg ctc gtc gag ggc atg ccc cag cac 1740
Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Met Pro Gln His
465 470 475 480
ggc acc gac cac ggg cag gtc gac ttc gcg gcg atc gcg gcc gcc gcg 1788
Gly Thr Asp His Gly Gln Val Asp Phe Ala Ala Ile Ala Ala Ala Ala
485 490 495
ggc atc cac tcc gtg ctc atc gac gac ccc gcc acc ctc cgc gag cag 1836
Gly Ile His Ser Val Leu Ile Asp Asp Pro Ala Thr Leu Arg Glu Gln
500 505 510
ctc gcc gcg gcg ctc gcc cac ccc ggg ccc gtg ctc atc gac gtc cgc 1884
Leu Ala Ala Ala Leu Ala His Pro Gly Pro Val Leu Ile Asp Val Arg
515 520 525
acc gac ccc gac gcc ctc tcc ctc ccg ccg gag atc acc tgg gag atg 1932
Thr Asp Pro Asp Ala Leu Ser Leu Pro Pro Glu Ile Thr Trp Glu Met
530 535 540
ctc atc ggc ttc tcc cgg gcc gcg acc cgt acc gtc ctc ggc ggc ggc 1980
Leu Ile Gly Phe Ser Arg Ala Ala Thr Arg Thr Val Leu Gly Gly Gly
545 550 555 560
gtc ggc gag atg gtg cac ctc gcg cgc acg aac ctg cgc aac atc ggc 2028
Val Gly Glu Met Val His Leu Ala Arg Thr Asn Leu Arg Asn Ile Gly
565 570 575
gcg gcc ggg gcg gtc tag tctgggcgtc atgaccccga ccgtccggat 2076
Ala Ala Gly Ala Val
580
cgacctcgcc cgcctcgacg cgaacatcgc cgcgcaaccc gggcggctgc gaccgcacgt 2136
gaagacccac aagatcctgg agatcgcccg ccggcagctc gcggcgggtg cccacggcct 2196
cacggtcgcg acggtggggg aggcggaggt cttcgccgag gtctgcgacg acatcttcat 2256
cgcctacccc gtctgggccg acaagaggct gcgcgacctg gccgggaggg tgcggctgag 2316
cgtcggctgc gactcgctgg ccgccgccga 2346
<210> 140
<211> 581
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 140
Met Ala Arg Thr Tyr Ala Gln Gln Leu Val Asp Ala Leu Glu Arg Gln
1 5 10 15
Gly Val Glu Arg Ile Tyr Gly Val Val Gly Asp Ser Leu Asn Pro Ile
20 25 30
Val Asp Ala Val Arg Thr Ser Pro Ile Glu Trp Ile His Val Arg Asn
35 40 45
Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Leu Thr Gly
50 55 60
Arg Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His Leu
65 70 75 80
Ile Gln Gly Leu Tyr Asp Ala His Arg Asn Gly Ala His Val Leu Ala
85 90 95
Leu Ala Ser His Ile Pro Ser Asn Gln Ile Gly Thr Ser Tyr Phe Gln
100 105 110
Glu Thr His Pro Glu Gln Leu Phe Ala Glu Cys Ser Gly Tyr Cys Glu
115 120 125
Met Val Asn Ser Ala Asp Gln Gly Ala Arg Val Leu His His Ala Ile
130 135 140
Gln Ser Thr Met Ala Gly Gln Gly Val Ser Val Leu Val Ile Pro Gly
145 150 155 160
Asp Ile Ala Thr Gln Asp Ala Gly Asp Asp Arg Phe Leu Gln Ser Thr
165 170 175
Val Ala Thr Gly Arg Pro Val Leu His Pro Asp Ala Arg Glu Ala Ala
180 185 190
Ala Leu Val Ala Ala Ile Asn Ala Ala Asp Thr Val Thr Leu Phe Cys
195 200 205
Gly Ala Gly Val Arg Asp Ala Arg Glu Gln Val Leu Ala Leu Ala Glu
210 215 220
Lys Ile Lys Ala Pro Ile Gly His Ala Leu Gly Gly Lys Met His Ile
225 230 235 240
Gln His Asp Asn Pro Phe Asp Val Gly Met Ser Gly Leu Leu Gly Tyr
245 250 255
Gly Ala Cys His Asp Ala Leu His Asp Ala Asp Leu Leu Ile Leu Leu
260 265 270
Gly Thr Asp Phe Pro Tyr Thr Glu Phe Leu Pro Thr Gly Asn Val Ala
275 280 285
Gln Val Asp Ser Asp Ala Ala His Ile Gly Arg Arg Thr Thr Val His
290 295 300
His Pro Val Val Gly Asp Val Gly Ala Thr Leu Asp Thr Ile Leu Pro
305 310 315 320
His Leu Glu Glu Lys Thr Asp Arg Ser Phe Leu Asp Gly Met Leu Arg
325 330 335
Lys His Ala Arg Thr Leu Glu Lys Val Val Glu Val Tyr Thr Arg Asp
340 345 350
Val Gly Lys His Thr Pro Ile His Pro Glu Tyr Leu Ala Ala Val Leu
355 360 365
Asp Glu Leu Ala Ala Asp Asp Ala Val Phe Thr Val Asp Thr Gly Met
370 375 380
Cys Asn Val Trp Ala Ala Arg Tyr Leu Thr Pro Asn Gly Arg Arg Ala
385 390 395 400
Gln Ile Gly Ser Leu Arg His Gly Thr Met Ala Asn Ala Leu Pro His
405 410 415
Ala Ile Gly Ala Gln Phe Ala Asp Arg Asn Arg Gln Val Ile Ser Leu
420 425 430
Asn Gly Asp Gly Gly Leu Ala Met Leu Leu Gly Glu Leu Leu Thr Val
435 440 445
Glu Leu His Asp Leu Pro Val Lys Met Val Val Phe Asn Asn Ser Ser
450 455 460
Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Met Pro Gln His
465 470 475 480
Gly Thr Asp His Gly Gln Val Asp Phe Ala Ala Ile Ala Ala Ala Ala
485 490 495
Gly Ile His Ser Val Leu Ile Asp Asp Pro Ala Thr Leu Arg Glu Gln
500 505 510
Leu Ala Ala Ala Leu Ala His Pro Gly Pro Val Leu Ile Asp Val Arg
515 520 525
Thr Asp Pro Asp Ala Leu Ser Leu Pro Pro Glu Ile Thr Trp Glu Met
530 535 540
Leu Ile Gly Phe Ser Arg Ala Ala Thr Arg Thr Val Leu Gly Gly Gly
545 550 555 560
Val Gly Glu Met Val His Leu Ala Arg Thr Asn Leu Arg Asn Ile Gly
565 570 575
Ala Ala Gly Ala Val
580
<210> 141
<211> 2010
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1710)
<223> ген пируваткиназы 1
<400> 141
acgagctgct gtggaacctc ctcatcttcg ggctgctcat cctcatcgac cgccgcttcc 60
gcatcgcacc gggtggactg ttcgccctct acgtcgccgg ttacacgctg ggccgtttct 120
ggatcgagct catgcgcgac gattccgcca cgatgatcct cggccagcgg gtcaacacct 180
gggtctccgc cgtggtgttc ctcgtctcga tcgcggtatt cgtgtggttg cagcgacgtc 240
agcgggctgt tgtccgagaa acagagtagg ttggtagccg tgtctagacg aaccaagatt 300
gtg tgc acc ctc ggt cct gcc gtc gcc tcc aag gac gcg atc acg cgt 348
Val Cys Thr Leu Gly Pro Ala Val Ala Ser Lys Asp Ala Ile Thr Arg
1 5 10 15
ctg gtg gag gac ggc atg gac gtc gcc cgc ctg aac ttc tcc cac ggt 396
Leu Val Glu Asp Gly Met Asp Val Ala Arg Leu Asn Phe Ser His Gly
20 25 30
gat cac ccc gat cac gag cag aac tac aag tgg gtc cgg gaa gcc acc 444
Asp His Pro Asp His Glu Gln Asn Tyr Lys Trp Val Arg Glu Ala Thr
35 40 45
gat gag acc ggc cgt gcc gtc ggc atc ctc gct gac ctc cag ggc ccg 492
Asp Glu Thr Gly Arg Ala Val Gly Ile Leu Ala Asp Leu Gln Gly Pro
50 55 60
aag atc cgc ctc ggt cgc ttc atc gac ggt gcc acc gtc tgg gag acc 540
Lys Ile Arg Leu Gly Arg Phe Ile Asp Gly Ala Thr Val Trp Glu Thr
65 70 75 80
ggt gag gtc atc cgc atc acc gtg gac gat gtc gag ggc acg cac gac 588
Gly Glu Val Ile Arg Ile Thr Val Asp Asp Val Glu Gly Thr His Asp
85 90 95
cgc gtg tcc acc acc tac aag aac ctc gcg cag gac gcc aag ccc ggc 636
Arg Val Ser Thr Thr Tyr Lys Asn Leu Ala Gln Asp Ala Lys Pro Gly
100 105 110
gac cgt ctg ctc gtc gac gac ggc aag gtc ggc ctg acc tgc ctc gag 684
Asp Arg Leu Leu Val Asp Asp Gly Lys Val Gly Leu Thr Cys Leu Glu
115 120 125
gtc gac ggc aac gac gtc gtc tgc gag gtc acc gag ggc ggc ccc gtc 732
Val Asp Gly Asn Asp Val Val Cys Glu Val Thr Glu Gly Gly Pro Val
130 135 140
tcc aac aac aag ggc gtc tcc ctc ccg ggc atg gac atc tct gtg ccg 780
Ser Asn Asn Lys Gly Val Ser Leu Pro Gly Met Asp Ile Ser Val Pro
145 150 155 160
gcg ctg tcg gag aag gac atc gcc gac ctc gag ttc gcc ctc aag ctg 828
Ala Leu Ser Glu Lys Asp Ile Ala Asp Leu Glu Phe Ala Leu Lys Leu
165 170 175
ggc gtc gac ttc atc gcc ctg tcc ttc gtc cgc tcc ccg gcg gac gtc 876
Gly Val Asp Phe Ile Ala Leu Ser Phe Val Arg Ser Pro Ala Asp Val
180 185 190
gac ctc gtc cac gag gtc atg gac cgc gtc ggc cgc cac gtg ccg gtc 924
Asp Leu Val His Glu Val Met Asp Arg Val Gly Arg His Val Pro Val
195 200 205
atc gcg aag ctg gag aag ccg gag gcc gtc cac gcc ctc gag tcc atc 972
Ile Ala Lys Leu Glu Lys Pro Glu Ala Val His Ala Leu Glu Ser Ile
210 215 220
gtc ctc gcc ttc gac gcc atc atg gtc gcc cgc ggt gac ctc ggc gtc 1020
Val Leu Ala Phe Asp Ala Ile Met Val Ala Arg Gly Asp Leu Gly Val
225 230 235 240
gag gtg ccg ctg gag cag gtc ccg ctg gtg cag aag cgg gcc atc cag 1068
Glu Val Pro Leu Glu Gln Val Pro Leu Val Gln Lys Arg Ala Ile Gln
245 250 255
atc gcc cgc gag aac gcg aag ccg gtc att gtc gcc acg cag atg ctc 1116
Ile Ala Arg Glu Asn Ala Lys Pro Val Ile Val Ala Thr Gln Met Leu
260 265 270
gac tcc atg atc gag aac tcc acc ccg acc cgc gcc gag gcc tcc gac 1164
Asp Ser Met Ile Glu Asn Ser Thr Pro Thr Arg Ala Glu Ala Ser Asp
275 280 285
gtc gcc aac gcc gtc ctc gac ggc gcc gac gcg gtc atg ctc tcc ggt 1212
Val Ala Asn Ala Val Leu Asp Gly Ala Asp Ala Val Met Leu Ser Gly
290 295 300
gag acc tcc atc ggt gtg gac ccg cac aac gtc gtg cgc acc atg gcc 1260
Glu Thr Ser Ile Gly Val Asp Pro His Asn Val Val Arg Thr Met Ala
305 310 315 320
cgc atc gtc acc cag gcc gag acc gac ggc cac gtg ccg ccg ctg agc 1308
Arg Ile Val Thr Gln Ala Glu Thr Asp Gly His Val Pro Pro Leu Ser
325 330 335
cat gtc ccg cgc acc aag cgg ggc gtc atc tcc tac tcg gcc cgt gac 1356
His Val Pro Arg Thr Lys Arg Gly Val Ile Ser Tyr Ser Ala Arg Asp
340 345 350
atc gcc gag cgt ctc aac gcc cgc gcc ctg gtc tgc ttc acc acc tcc 1404
Ile Ala Glu Arg Leu Asn Ala Arg Ala Leu Val Cys Phe Thr Thr Ser
355 360 365
ggt gac acc gcc cgc cgt gtc gcc cgc ctg cac tcg cac ctg ccg ctg 1452
Gly Asp Thr Ala Arg Arg Val Ala Arg Leu His Ser His Leu Pro Leu
370 375 380
ctg gtg ttc acc ccg cac ccg gcg gtc cgt tcg cag ctg gcc ctc acc 1500
Leu Val Phe Thr Pro His Pro Ala Val Arg Ser Gln Leu Ala Leu Thr
385 390 395 400
tgg ggc gcc gag acc ttc ctc tgc gcc cag gcc ctg tcc acc gac gac 1548
Trp Gly Ala Glu Thr Phe Leu Cys Ala Gln Ala Leu Ser Thr Asp Asp
405 410 415
atg atg gcc gag ctg gac cgt tcc ctg ctc gcc atg gac gac tac cac 1596
Met Met Ala Glu Leu Asp Arg Ser Leu Leu Ala Met Asp Asp Tyr His
420 425 430
cgc gac gac atg atg gtc gtc gtc gcc ggc acc ccg ccc ggg gtc tcc 1644
Arg Asp Asp Met Met Val Val Val Ala Gly Thr Pro Pro Gly Val Ser
435 440 445
ggc aac acg aac atg atc cac gtc cac ctg ctg ggc gag gac acg agt 1692
Gly Asn Thr Asn Met Ile His Val His Leu Leu Gly Glu Asp Thr Ser
450 455 460
tcc ccg acc ggc agg tag ccggagaaca gaaaaaggag cggcggagag 1740
Ser Pro Thr Gly Arg
465
gatcatcctc tccgccgctc ctgtcgtgtg cggctacttc accggggtcg gctccagctt 1800
ccacacctca cgcgcgtagt cgttgatggt gcggtcggag gagaaacggc ccgaggcgca 1860
gatgttcgcc cagcacatac gcgcccagtg caggcggtcg gcgtagtcgg cggccatgcg 1920
gtcgcgggtc tcgcggtagt cggcgaagtc accgaggacg tagtagacgt ccggggtctc 1980
ccagccgccg ccgtcgagaa gcgaggaacg 2010
<210> 142
<211> 469
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 142
Val Cys Thr Leu Gly Pro Ala Val Ala Ser Lys Asp Ala Ile Thr Arg
1 5 10 15
Leu Val Glu Asp Gly Met Asp Val Ala Arg Leu Asn Phe Ser His Gly
20 25 30
Asp His Pro Asp His Glu Gln Asn Tyr Lys Trp Val Arg Glu Ala Thr
35 40 45
Asp Glu Thr Gly Arg Ala Val Gly Ile Leu Ala Asp Leu Gln Gly Pro
50 55 60
Lys Ile Arg Leu Gly Arg Phe Ile Asp Gly Ala Thr Val Trp Glu Thr
65 70 75 80
Gly Glu Val Ile Arg Ile Thr Val Asp Asp Val Glu Gly Thr His Asp
85 90 95
Arg Val Ser Thr Thr Tyr Lys Asn Leu Ala Gln Asp Ala Lys Pro Gly
100 105 110
Asp Arg Leu Leu Val Asp Asp Gly Lys Val Gly Leu Thr Cys Leu Glu
115 120 125
Val Asp Gly Asn Asp Val Val Cys Glu Val Thr Glu Gly Gly Pro Val
130 135 140
Ser Asn Asn Lys Gly Val Ser Leu Pro Gly Met Asp Ile Ser Val Pro
145 150 155 160
Ala Leu Ser Glu Lys Asp Ile Ala Asp Leu Glu Phe Ala Leu Lys Leu
165 170 175
Gly Val Asp Phe Ile Ala Leu Ser Phe Val Arg Ser Pro Ala Asp Val
180 185 190
Asp Leu Val His Glu Val Met Asp Arg Val Gly Arg His Val Pro Val
195 200 205
Ile Ala Lys Leu Glu Lys Pro Glu Ala Val His Ala Leu Glu Ser Ile
210 215 220
Val Leu Ala Phe Asp Ala Ile Met Val Ala Arg Gly Asp Leu Gly Val
225 230 235 240
Glu Val Pro Leu Glu Gln Val Pro Leu Val Gln Lys Arg Ala Ile Gln
245 250 255
Ile Ala Arg Glu Asn Ala Lys Pro Val Ile Val Ala Thr Gln Met Leu
260 265 270
Asp Ser Met Ile Glu Asn Ser Thr Pro Thr Arg Ala Glu Ala Ser Asp
275 280 285
Val Ala Asn Ala Val Leu Asp Gly Ala Asp Ala Val Met Leu Ser Gly
290 295 300
Glu Thr Ser Ile Gly Val Asp Pro His Asn Val Val Arg Thr Met Ala
305 310 315 320
Arg Ile Val Thr Gln Ala Glu Thr Asp Gly His Val Pro Pro Leu Ser
325 330 335
His Val Pro Arg Thr Lys Arg Gly Val Ile Ser Tyr Ser Ala Arg Asp
340 345 350
Ile Ala Glu Arg Leu Asn Ala Arg Ala Leu Val Cys Phe Thr Thr Ser
355 360 365
Gly Asp Thr Ala Arg Arg Val Ala Arg Leu His Ser His Leu Pro Leu
370 375 380
Leu Val Phe Thr Pro His Pro Ala Val Arg Ser Gln Leu Ala Leu Thr
385 390 395 400
Trp Gly Ala Glu Thr Phe Leu Cys Ala Gln Ala Leu Ser Thr Asp Asp
405 410 415
Met Met Ala Glu Leu Asp Arg Ser Leu Leu Ala Met Asp Asp Tyr His
420 425 430
Arg Asp Asp Met Met Val Val Val Ala Gly Thr Pro Pro Gly Val Ser
435 440 445
Gly Asn Thr Asn Met Ile His Val His Leu Leu Gly Glu Asp Thr Ser
450 455 460
Ser Pro Thr Gly Arg
465
<210> 143
<211> 2472
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(2172)
<223> ген пируваткиназы 2
<400> 143
atccagaacc aggacgtcgc tctgccggtg tccgcgtacc tcgacggcga gtacgggcag 60
aaggacctct acatcggcac cccggcgatc atcaaccgcg ccggaatcgc ccgcacggtc 120
gagctgaagc tcaacgagca cgaggcggag cgcttcgccc actcggctga cacgctgcgc 180
gcgatcaagg aggagttctt ccccgccgac tgacggcttc cccgagatgc cgaacctccc 240
gaacacaggc acaatatggg ggtatcaccc attttgtcgt gcgcgtacgg gaggacagcc 300
gtg gat gcc agg ctg cag gaa ctg atc acc gag gtc gac ggt ctc atc 348
Val Asp Ala Arg Leu Gln Glu Leu Ile Thr Glu Val Asp Gly Leu Ile
1 5 10 15
gac tgc ctc aag gag gtc gag gag cag gtg gcc ccc gcg atc ggg cgg 396
Asp Cys Leu Lys Glu Val Glu Glu Gln Val Ala Pro Ala Ile Gly Arg
20 25 30
gtc gcc gag aac cac cgc cac ggc gcg gtg aac ctc gtc cac tac gcc 444
Val Ala Glu Asn His Arg His Gly Ala Val Asn Leu Val His Tyr Ala
35 40 45
gag ctg cgg cag cac gac gtc cgc gcg gtc cag ggc ggc ctg gcc tcc 492
Glu Leu Arg Gln His Asp Val Arg Ala Val Gln Gly Gly Leu Ala Ser
50 55 60
atc ggc gcc acc cgc ctg tcg acg gcg gag ccg gcc gtc ctc gcc cgt 540
Ile Gly Ala Thr Arg Leu Ser Thr Ala Glu Pro Ala Val Leu Ala Arg
65 70 75 80
ctc cac gcg gcg cgg aac gtg ctc tcc gcc tac aac ggt gag cag ctc 588
Leu His Ala Ala Arg Asn Val Leu Ser Ala Tyr Asn Gly Glu Gln Leu
85 90 95
aag tac acc ggc agc gag gtc cgt gac gcc ttc gcc acc gcc gac gac 636
Lys Tyr Thr Gly Ser Glu Val Arg Asp Ala Phe Ala Thr Ala Asp Asp
100 105 110
atc ctc gag gac cac gcc ctg cag ctc ctc ggc tac tcc tcc gag gag 684
Ile Leu Glu Asp His Ala Leu Gln Leu Leu Gly Tyr Ser Ser Glu Glu
115 120 125
acg cac tcc cgc atc atg gtg acc ctg ccg acg gag gca ggc gag gac 732
Thr His Ser Arg Ile Met Val Thr Leu Pro Thr Glu Ala Gly Glu Asp
130 135 140
ctc gac ctg gtc cgc ggt ttc gtc gag gcc ggc atg gag ctg gcc cgc 780
Leu Asp Leu Val Arg Gly Phe Val Glu Ala Gly Met Glu Leu Ala Arg
145 150 155 160
atc aac tgc gcc cac gac ggc ccc gag gtg tgg agg cgc atg atc gac 828
Ile Asn Cys Ala His Asp Gly Pro Glu Val Trp Arg Arg Met Ile Asp
165 170 175
aac gtc cac acc gcc gcc cgc gag gcc ggc cgg gag atc agg atc gcc 876
Asn Val His Thr Ala Ala Arg Glu Ala Gly Arg Glu Ile Arg Ile Ala
180 185 190
atg gac ctg gcg ggc ccg aag gtc cgc acc ggg gag atc gag ccg ggc 924
Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Glu Pro Gly
195 200 205
ccg gag gtc ggc cgc gcc cgc gtc acc cgc gag gac acc ggc gtg gtg 972
Pro Glu Val Gly Arg Ala Arg Val Thr Arg Glu Asp Thr Gly Val Val
210 215 220
gtc acc ccg tcg aag atc tgg ctc acc ccc gcc gac ctg gcc gac gac 1020
Val Thr Pro Ser Lys Ile Trp Leu Thr Pro Ala Asp Leu Ala Asp Asp
225 230 235 240
gag gtc ccg ccg gtc ccc gcc gga ctg ccg ggc cgc ccg acg ctg ccg 1068
Glu Val Pro Pro Val Pro Ala Gly Leu Pro Gly Arg Pro Thr Leu Pro
245 250 255
ctg cgc gtc gag cgt gac tgg ctg gag gcc ctg gag atc ggc tcc gag 1116
Leu Arg Val Glu Arg Asp Trp Leu Glu Ala Leu Glu Ile Gly Ser Glu
260 265 270
atc acc ttc cac gac aac cgc gac gcg aag cgc cgg ttc acc gtc acc 1164
Ile Thr Phe His Asp Asn Arg Asp Ala Lys Arg Arg Phe Thr Val Thr
275 280 285
cgc atc gag ggg cag ggg gtg ctg ctg gag ggg cgt cgc aac gcc tac 1212
Arg Ile Glu Gly Gln Gly Val Leu Leu Glu Gly Arg Arg Asn Ala Tyr
290 295 300
gtc tcc aac tcc acc ctc ctc gag acc gac tgg acc aag acc cgc gtc 1260
Val Ser Asn Ser Thr Leu Leu Glu Thr Asp Trp Thr Lys Thr Arg Val
305 310 315 320
tcc ggc gtc ccg ccg acc gag cag cgc ctg cgt ctg agc atc gga gac 1308
Ser Gly Val Pro Pro Thr Glu Gln Arg Leu Arg Leu Ser Ile Gly Asp
325 330 335
acg ctc atc ctc acc gac gac cag acc ccc gcc gtc gtc gcc ccg gac 1356
Thr Leu Ile Leu Thr Asp Asp Gln Thr Pro Ala Val Val Ala Pro Asp
340 345 350
gtc acc ccg cgc atc ggc tgc acg ctg ccg gag gcc gtc gcc gcc atc 1404
Val Thr Pro Arg Ile Gly Cys Thr Leu Pro Glu Ala Val Ala Ala Ile
355 360 365
gag gtc ggg cag aag gtg ctt ttc gac gac ggc tcg atc gcc gcc cgc 1452
Glu Val Gly Gln Lys Val Leu Phe Asp Asp Gly Ser Ile Ala Ala Arg
370 375 380
gcc gtc gac aag cag gtc acg gag cag ggc cac cac gag gtg cgc ctc 1500
Ala Val Asp Lys Gln Val Thr Glu Gln Gly His His Glu Val Arg Leu
385 390 395 400
gag gtg acc tac gcc ggg ccg gag ggc acc aag ctc gcc gcg tac aag 1548
Glu Val Thr Tyr Ala Gly Pro Glu Gly Thr Lys Leu Ala Ala Tyr Lys
405 410 415
ggc atc aac ctg ccg gac acc gtc ctg ccg ctg ccg agc ctc acc gag 1596
Gly Ile Asn Leu Pro Asp Thr Val Leu Pro Leu Pro Ser Leu Thr Glu
420 425 430
gag gac atc gag gcg ttc cgc ttc gtg gcc gag cac ggt gac atc gcg 1644
Glu Asp Ile Glu Ala Phe Arg Phe Val Ala Glu His Gly Asp Ile Ala
435 440 445
aac gtc tcc ttc att cgc gac gcc gac gac gtc aac ttc gtg ctc gac 1692
Asn Val Ser Phe Ile Arg Asp Ala Asp Asp Val Asn Phe Val Leu Asp
450 455 460
acc ctc gag cag atc gcg cag gag tcc gac gac ccg gag cgc gtc cgc 1740
Thr Leu Glu Gln Ile Ala Gln Glu Ser Asp Asp Pro Glu Arg Val Arg
465 470 475 480
aac ctc ggc atc gtg ctc aag atc gag acg atc ccc gcc tac gag ggc 1788
Asn Leu Gly Ile Val Leu Lys Ile Glu Thr Ile Pro Ala Tyr Glu Gly
485 490 495
ctg tgc agc gtc ctg ctc gcc ggc atg cgc cac ccc aac ctc gga gtc 1836
Leu Cys Ser Val Leu Leu Ala Gly Met Arg His Pro Asn Leu Gly Val
500 505 510
atg atc gcc cgc ggc gac ctg gcc gtc gag ctc ggc ttc gag cgc atg 1884
Met Ile Ala Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Glu Arg Met
515 520 525
gcc gag gtc ccg cgc ctc atc gcc cag atg gcg gag gcc gcc cac gtg 1932
Ala Glu Val Pro Arg Leu Ile Ala Gln Met Ala Glu Ala Ala His Val
530 535 540
ccc gtc atc atg gcc acc cag gtg ctc gag aac ctg gcc aag tcc ggc 1980
Pro Val Ile Met Ala Thr Gln Val Leu Glu Asn Leu Ala Lys Ser Gly
545 550 555 560
ctg ccg acc cgc gcc gag atc acc gac gcc gcg tac gcc ctg cgc tcc 2028
Leu Pro Thr Arg Ala Glu Ile Thr Asp Ala Ala Tyr Ala Leu Arg Ser
565 570 575
gag gcc gtg atg ctc aac aag ggc ccg cac atc acc gac gcg atc cgc 2076
Glu Ala Val Met Leu Asn Lys Gly Pro His Ile Thr Asp Ala Ile Arg
580 585 590
atc ctc cac acc ctg tcg acc aag ctc ggc cgt tcc cag cgc aag aac 2124
Ile Leu His Thr Leu Ser Thr Lys Leu Gly Arg Ser Gln Arg Lys Asn
595 600 605
cgc cag atg ctc cgc cag atc tcg agc tgg atg ccc ggg aat gag tag 2172
Arg Gln Met Leu Arg Gln Ile Ser Ser Trp Met Pro Gly Asn Glu
610 615 620
atgaccgccg cacccaggat catcgcccac cgcggttact ccgcgaagta ccccgagttg 2232
acgcccgtcg ccttcgagcg ggcgctggaa ctgcccgtcc acggcatcga gtgcgatgta 2292
cgcctcagcc tcgacgggga ggtggtggtg ttgcatgacc cgctcatcga ccgcgtcgcg 2352
gacggctccg ggcgggtctc ccgcatgacg gtggacgccc tgcgtgacca caactacggc 2412
accgccgagc tgccgcagga catgctcacc ctcccggagc tgctggagat ggtggagccg 2472
<210> 144
<211> 623
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 144
Val Asp Ala Arg Leu Gln Glu Leu Ile Thr Glu Val Asp Gly Leu Ile
1 5 10 15
Asp Cys Leu Lys Glu Val Glu Glu Gln Val Ala Pro Ala Ile Gly Arg
20 25 30
Val Ala Glu Asn His Arg His Gly Ala Val Asn Leu Val His Tyr Ala
35 40 45
Glu Leu Arg Gln His Asp Val Arg Ala Val Gln Gly Gly Leu Ala Ser
50 55 60
Ile Gly Ala Thr Arg Leu Ser Thr Ala Glu Pro Ala Val Leu Ala Arg
65 70 75 80
Leu His Ala Ala Arg Asn Val Leu Ser Ala Tyr Asn Gly Glu Gln Leu
85 90 95
Lys Tyr Thr Gly Ser Glu Val Arg Asp Ala Phe Ala Thr Ala Asp Asp
100 105 110
Ile Leu Glu Asp His Ala Leu Gln Leu Leu Gly Tyr Ser Ser Glu Glu
115 120 125
Thr His Ser Arg Ile Met Val Thr Leu Pro Thr Glu Ala Gly Glu Asp
130 135 140
Leu Asp Leu Val Arg Gly Phe Val Glu Ala Gly Met Glu Leu Ala Arg
145 150 155 160
Ile Asn Cys Ala His Asp Gly Pro Glu Val Trp Arg Arg Met Ile Asp
165 170 175
Asn Val His Thr Ala Ala Arg Glu Ala Gly Arg Glu Ile Arg Ile Ala
180 185 190
Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Glu Pro Gly
195 200 205
Pro Glu Val Gly Arg Ala Arg Val Thr Arg Glu Asp Thr Gly Val Val
210 215 220
Val Thr Pro Ser Lys Ile Trp Leu Thr Pro Ala Asp Leu Ala Asp Asp
225 230 235 240
Glu Val Pro Pro Val Pro Ala Gly Leu Pro Gly Arg Pro Thr Leu Pro
245 250 255
Leu Arg Val Glu Arg Asp Trp Leu Glu Ala Leu Glu Ile Gly Ser Glu
260 265 270
Ile Thr Phe His Asp Asn Arg Asp Ala Lys Arg Arg Phe Thr Val Thr
275 280 285
Arg Ile Glu Gly Gln Gly Val Leu Leu Glu Gly Arg Arg Asn Ala Tyr
290 295 300
Val Ser Asn Ser Thr Leu Leu Glu Thr Asp Trp Thr Lys Thr Arg Val
305 310 315 320
Ser Gly Val Pro Pro Thr Glu Gln Arg Leu Arg Leu Ser Ile Gly Asp
325 330 335
Thr Leu Ile Leu Thr Asp Asp Gln Thr Pro Ala Val Val Ala Pro Asp
340 345 350
Val Thr Pro Arg Ile Gly Cys Thr Leu Pro Glu Ala Val Ala Ala Ile
355 360 365
Glu Val Gly Gln Lys Val Leu Phe Asp Asp Gly Ser Ile Ala Ala Arg
370 375 380
Ala Val Asp Lys Gln Val Thr Glu Gln Gly His His Glu Val Arg Leu
385 390 395 400
Glu Val Thr Tyr Ala Gly Pro Glu Gly Thr Lys Leu Ala Ala Tyr Lys
405 410 415
Gly Ile Asn Leu Pro Asp Thr Val Leu Pro Leu Pro Ser Leu Thr Glu
420 425 430
Glu Asp Ile Glu Ala Phe Arg Phe Val Ala Glu His Gly Asp Ile Ala
435 440 445
Asn Val Ser Phe Ile Arg Asp Ala Asp Asp Val Asn Phe Val Leu Asp
450 455 460
Thr Leu Glu Gln Ile Ala Gln Glu Ser Asp Asp Pro Glu Arg Val Arg
465 470 475 480
Asn Leu Gly Ile Val Leu Lys Ile Glu Thr Ile Pro Ala Tyr Glu Gly
485 490 495
Leu Cys Ser Val Leu Leu Ala Gly Met Arg His Pro Asn Leu Gly Val
500 505 510
Met Ile Ala Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Glu Arg Met
515 520 525
Ala Glu Val Pro Arg Leu Ile Ala Gln Met Ala Glu Ala Ala His Val
530 535 540
Pro Val Ile Met Ala Thr Gln Val Leu Glu Asn Leu Ala Lys Ser Gly
545 550 555 560
Leu Pro Thr Arg Ala Glu Ile Thr Asp Ala Ala Tyr Ala Leu Arg Ser
565 570 575
Glu Ala Val Met Leu Asn Lys Gly Pro His Ile Thr Asp Ala Ile Arg
580 585 590
Ile Leu His Thr Leu Ser Thr Lys Leu Gly Arg Ser Gln Arg Lys Asn
595 600 605
Arg Gln Met Leu Arg Gln Ile Ser Ser Trp Met Pro Gly Asn Glu
610 615 620
<210> 145
<211> 1878
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1578)
<223> ген енолазы
<400> 145
ccctgggggc ggcgcacctg ctgtgtacca acggtgggga cctcagcacc ccggagggct 60
ggtcccgggc gatctacgcc tacaaccgtt ccgaggccta cctcatcgac gtgcgtgacg 120
cggccgcgtc ctatgccctg aaccagccgg cccggtgacg gccacccctg gttgccccct 180
gcaatcgtcg ggttgtagcc cctggtggcg gtttttctgg aagaattgtc cccggacgcg 240
tgtcgagagc gcgcgacgat gacgaactcc catctcatat ggctatagga ggccccaaaa 300
gtg gct gac atc att cac gtt ttc gca cga gag atc atg gat tcc cgt 348
Val Ala Asp Ile Ile His Val Phe Ala Arg Glu Ile Met Asp Ser Arg
1 5 10 15
ggc aac ccg acc gtc gag gcc ggc gtt gtg ctg gat gac gga acc ctc 396
Gly Asn Pro Thr Val Glu Ala Gly Val Val Leu Asp Asp Gly Thr Leu
20 25 30
gcc acc gcc ggt gtc ccc tcc ggc gcc tcc acc ggt gtc cac gag gca 444
Ala Thr Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala
35 40 45
cac gag ctg cgt gac ggc ggc gac cgc tac ctg ggc aag ggt gtc ctg 492
His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu
50 55 60
aag gcc gtc gag aac gtc aac gag gag atc gcc gac gag ctg gcc ggc 540
Lys Ala Val Glu Asn Val Asn Glu Glu Ile Ala Asp Glu Leu Ala Gly
65 70 75 80
ttc gag gcc gat gac cag cgc gcc atc gac cag gcc atg atc cag ctg 588
Phe Glu Ala Asp Asp Gln Arg Ala Ile Asp Gln Ala Met Ile Gln Leu
85 90 95
gac ggc acc gac aac aag gcc cgc ctg ggt gcc aac gcc atc ctg ggt 636
Asp Gly Thr Asp Asn Lys Ala Arg Leu Gly Ala Asn Ala Ile Leu Gly
100 105 110
gtc tcc atg gct gtg gcc cgc gcc gcc gcc gag tcc gcc ggc ctg ccg 684
Val Ser Met Ala Val Ala Arg Ala Ala Ala Glu Ser Ala Gly Leu Pro
115 120 125
ctc tac cgt tac gtc ggt ggc ccg aac gca cac gtg ctg ccg gtc ccg 732
Leu Tyr Arg Tyr Val Gly Gly Pro Asn Ala His Val Leu Pro Val Pro
130 135 140
atg atg aac atc ctc aac ggt ggc gcc cac gcc gac tcc ggc gtc gac 780
Met Met Asn Ile Leu Asn Gly Gly Ala His Ala Asp Ser Gly Val Asp
145 150 155 160
gtc cag gag ttc atg atc gcc ccg atc ggt gcc gag acc ttc tcc gag 828
Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu
165 170 175
gct ctg cag gtc ggc gcc gag gtg tac cac acc ctg aag tcc gtc atc 876
Ala Leu Gln Val Gly Ala Glu Val Tyr His Thr Leu Lys Ser Val Ile
180 185 190
cag gag aag ggc ctg tcc acc ggc ctg ggc gac gag ggc ggt ttc gcc 924
Gln Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala
195 200 205
ccg tcc gtc gag tcc acc cgt gcg gcc ctg gac ctc atc gtc gag gcc 972
Pro Ser Val Glu Ser Thr Arg Ala Ala Leu Asp Leu Ile Val Glu Ala
210 215 220
gtc gag aag gcc ggc tac aag ctg ggc gac gat gtc gcc ctg gcc ctg 1020
Val Glu Lys Ala Gly Tyr Lys Leu Gly Asp Asp Val Ala Leu Ala Leu
225 230 235 240
gac gtc gcc tcc tcc gag ttc ttc aag gac ggc aag tac cac ttc gag 1068
Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Lys Tyr His Phe Glu
245 250 255
ggc aag gag ctc tcc gcc gag gag atg tcc aag gtc tac gag gag ctc 1116
Gly Lys Glu Leu Ser Ala Glu Glu Met Ser Lys Val Tyr Glu Glu Leu
260 265 270
atc aac gag tac ccg atc gtc tcc atc gag gat ccg ctg cag gag gac 1164
Ile Asn Glu Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp
275 280 285
gac tgg gac ggc tac gtc gcc ctg acc gcc gcc atc ggt gac aag gtc 1212
Asp Trp Asp Gly Tyr Val Ala Leu Thr Ala Ala Ile Gly Asp Lys Val
290 295 300
cag ctg gtc ggc gac gac ttc ttc gtc acc aac ccg gcc cgc ctg aag 1260
Gln Leu Val Gly Asp Asp Phe Phe Val Thr Asn Pro Ala Arg Leu Lys
305 310 315 320
gag ggc atc gcc aag aag gcc gcc aac tcc atc ctg gtg aag gtc aac 1308
Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn
325 330 335
cag atc ggt acc ctc acc gag acc ttc gac gcc gtc gag atg gcc cac 1356
Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Glu Met Ala His
340 345 350
cgt gcg ggc tac acc tcg atg atg tcc cac cgt tcc ggt gag acc gag 1404
Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu
355 360 365
gac acc acc atc gcc gac ctg gct gtc gca ctg aac tgc ggc cag atc 1452
Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile
370 375 380
aag acc ggt gcc ccg gcc cgc tcc gag cgc gtc gcc aag tac aac cag 1500
Lys Thr Gly Ala Pro Ala Arg Ser Glu Arg Val Ala Lys Tyr Asn Gln
385 390 395 400
ctg ctg cgc atc gag cag gag ctc ggc gac gcc gcc gtc tac gcc ggc 1548
Leu Leu Arg Ile Glu Gln Glu Leu Gly Asp Ala Ala Val Tyr Ala Gly
405 410 415
cgc tcc gcc ttc ccg cgc ttc cag aag taa ctgacagcgc tttccgacgc 1598
Arg Ser Ala Phe Pro Arg Phe Gln Lys
420 425
ccccgtgcac cccggtgcac gggggcgtcc ccgtatccgt gggggaggac accatggcat 1658
tcttcggcag acgtagggca cggccccggc tggagcccga cttcgtgcac acctggaccg 1718
gtgagcagct ggtccggatc gcggagggcg tcgacctggg caccctccgg gcggtgtacg 1778
ggcgtctcga cgccacctca cggatccact tcctcttcga cttcggtgcc ctcttctccg 1838
acgggtacct cttcgagctc tgggagcggc gtctcctgga 1878
<210> 146
<211> 425
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 146
Val Ala Asp Ile Ile His Val Phe Ala Arg Glu Ile Met Asp Ser Arg
1 5 10 15
Gly Asn Pro Thr Val Glu Ala Gly Val Val Leu Asp Asp Gly Thr Leu
20 25 30
Ala Thr Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala
35 40 45
His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu
50 55 60
Lys Ala Val Glu Asn Val Asn Glu Glu Ile Ala Asp Glu Leu Ala Gly
65 70 75 80
Phe Glu Ala Asp Asp Gln Arg Ala Ile Asp Gln Ala Met Ile Gln Leu
85 90 95
Asp Gly Thr Asp Asn Lys Ala Arg Leu Gly Ala Asn Ala Ile Leu Gly
100 105 110
Val Ser Met Ala Val Ala Arg Ala Ala Ala Glu Ser Ala Gly Leu Pro
115 120 125
Leu Tyr Arg Tyr Val Gly Gly Pro Asn Ala His Val Leu Pro Val Pro
130 135 140
Met Met Asn Ile Leu Asn Gly Gly Ala His Ala Asp Ser Gly Val Asp
145 150 155 160
Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu
165 170 175
Ala Leu Gln Val Gly Ala Glu Val Tyr His Thr Leu Lys Ser Val Ile
180 185 190
Gln Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala
195 200 205
Pro Ser Val Glu Ser Thr Arg Ala Ala Leu Asp Leu Ile Val Glu Ala
210 215 220
Val Glu Lys Ala Gly Tyr Lys Leu Gly Asp Asp Val Ala Leu Ala Leu
225 230 235 240
Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Lys Tyr His Phe Glu
245 250 255
Gly Lys Glu Leu Ser Ala Glu Glu Met Ser Lys Val Tyr Glu Glu Leu
260 265 270
Ile Asn Glu Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp
275 280 285
Asp Trp Asp Gly Tyr Val Ala Leu Thr Ala Ala Ile Gly Asp Lys Val
290 295 300
Gln Leu Val Gly Asp Asp Phe Phe Val Thr Asn Pro Ala Arg Leu Lys
305 310 315 320
Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn
325 330 335
Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Glu Met Ala His
340 345 350
Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu
355 360 365
Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile
370 375 380
Lys Thr Gly Ala Pro Ala Arg Ser Glu Arg Val Ala Lys Tyr Asn Gln
385 390 395 400
Leu Leu Arg Ile Glu Gln Glu Leu Gly Asp Ala Ala Val Tyr Ala Gly
405 410 415
Arg Ser Ala Phe Pro Arg Phe Gln Lys
420 425
<210> 147
<211> 1347
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1047)
<223> ген gpmA
<400> 147
tcgccgtctc cgagggggag acgggagtgc ttgtcgacgg ccacgacacc ggcgactggg 60
ccgacgccct ggagaacctc ctcgacgacg acgacacccg cctgcggatg gcgaccgacg 120
ccgtccagca cgccgcgaac ttctcgtggt cgaactccgc cgcccagctg gcggcggtgt 180
acgccgaggc cctgagcctg gaggtcgaca actgcccgca acgccgcgaa cgggccgaag 240
ggctctaaac ggggggtatt aatccgcacg ctaccggcgg aatcgtggca tattggagac 300
atg acc aac gga aag ctc att ctt ctt cgc cac ggg cag agc cag tgg 348
Met Thr Asn Gly Lys Leu Ile Leu Leu Arg His Gly Gln Ser Gln Trp
1 5 10 15
aac aaa tcg aac cag ttc acc ggc tgg gtg gac gtt gac ctg acc gag 396
Asn Lys Ser Asn Gln Phe Thr Gly Trp Val Asp Val Asp Leu Thr Glu
20 25 30
cag ggc gtc gcc gag gct gtc cgc ggc ggc gag atg ctc aag gag gct 444
Gln Gly Val Ala Glu Ala Val Arg Gly Gly Glu Met Leu Lys Glu Ala
35 40 45
ggt ctc ctg ccg gac atc gtc tac acc tcc ctg ctg cgt cgt gcc atc 492
Gly Leu Leu Pro Asp Ile Val Tyr Thr Ser Leu Leu Arg Arg Ala Ile
50 55 60
cgc acc gcg aac atc gcg ctc aac gcc gcc gac cgt cac tgg atc ccg 540
Arg Thr Ala Asn Ile Ala Leu Asn Ala Ala Asp Arg His Trp Ile Pro
65 70 75 80
gtc gtc cgc gac tgg cgt ctc aac gag cgc cac tac ggt gcg ctg cag 588
Val Val Arg Asp Trp Arg Leu Asn Glu Arg His Tyr Gly Ala Leu Gln
85 90 95
ggc ctg gac aag gcc gag acc aag gac aag tac ggc gac gag cag ttc 636
Gly Leu Asp Lys Ala Glu Thr Lys Asp Lys Tyr Gly Asp Glu Gln Phe
100 105 110
atg gcg tgg cgt cgt tcc tac gac acc ccg ccg ccg gag ctg gcc gac 684
Met Ala Trp Arg Arg Ser Tyr Asp Thr Pro Pro Pro Glu Leu Ala Asp
115 120 125
gac gcc gag tac tcc cag tcc gac gat gtg cgc tac gcc gcc ctg gag 732
Asp Ala Glu Tyr Ser Gln Ser Asp Asp Val Arg Tyr Ala Ala Leu Glu
130 135 140
aac gca ccg cgt acc gag tgc ctc ctg gac gtc gtc aag cgt ttc atc 780
Asn Ala Pro Arg Thr Glu Cys Leu Leu Asp Val Val Lys Arg Phe Ile
145 150 155 160
ccg tac tac cag gag gag atc gag ccg cgc gtc aag cgt ggc gag acc 828
Pro Tyr Tyr Gln Glu Glu Ile Glu Pro Arg Val Lys Arg Gly Glu Thr
165 170 175
gtc ctc gtc gcc gcg cac ggc aac tcc ctg cgc gcg ctg gtc aag cac 876
Val Leu Val Ala Ala His Gly Asn Ser Leu Arg Ala Leu Val Lys His
180 185 190
ctg gac aac atc tcg gac gag gac atc gcg gcc ctc aac atc ccg acc 924
Leu Asp Asn Ile Ser Asp Glu Asp Ile Ala Ala Leu Asn Ile Pro Thr
195 200 205
ggc atc ccg ctg gtc tac gag ctg gat gct gac ggc aag gtc gtc aac 972
Gly Ile Pro Leu Val Tyr Glu Leu Asp Ala Asp Gly Lys Val Val Asn
210 215 220
ccg ggc ggc acc tac ctc gac ccc gag gcg gcc gcc gcc ggt gct gcc 1020
Pro Gly Gly Thr Tyr Leu Asp Pro Glu Ala Ala Ala Ala Gly Ala Ala
225 230 235 240
gct gtc gcg gca cag ggt ggc aag tag cctgtagggc gtgtccgccg 1067
Ala Val Ala Ala Gln Gly Gly Lys
245
ttctcgcgtt cctgttgggc gtcgccgtca ccggcgtcgc ctttccggcg ttgaactggg 1127
cacgtaagcg gatagtccgc caccgttcct cggccacgct cgaggacaac caggtcacca 1187
ccgtcggcca ggtcctgcac ctggccatcc agggttcccc cacgggcgtc accgtcgtcg 1247
accgcggcgg tgaggtgatc ctgtcgaacg cccgcgccca cgagatggcc ctcgtccacg 1307
accgcaccct caacgagcag gtgtggcagg tggcggggga 1347
<210> 148
<211> 248
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 148
Met Thr Asn Gly Lys Leu Ile Leu Leu Arg His Gly Gln Ser Gln Trp
1 5 10 15
Asn Lys Ser Asn Gln Phe Thr Gly Trp Val Asp Val Asp Leu Thr Glu
20 25 30
Gln Gly Val Ala Glu Ala Val Arg Gly Gly Glu Met Leu Lys Glu Ala
35 40 45
Gly Leu Leu Pro Asp Ile Val Tyr Thr Ser Leu Leu Arg Arg Ala Ile
50 55 60
Arg Thr Ala Asn Ile Ala Leu Asn Ala Ala Asp Arg His Trp Ile Pro
65 70 75 80
Val Val Arg Asp Trp Arg Leu Asn Glu Arg His Tyr Gly Ala Leu Gln
85 90 95
Gly Leu Asp Lys Ala Glu Thr Lys Asp Lys Tyr Gly Asp Glu Gln Phe
100 105 110
Met Ala Trp Arg Arg Ser Tyr Asp Thr Pro Pro Pro Glu Leu Ala Asp
115 120 125
Asp Ala Glu Tyr Ser Gln Ser Asp Asp Val Arg Tyr Ala Ala Leu Glu
130 135 140
Asn Ala Pro Arg Thr Glu Cys Leu Leu Asp Val Val Lys Arg Phe Ile
145 150 155 160
Pro Tyr Tyr Gln Glu Glu Ile Glu Pro Arg Val Lys Arg Gly Glu Thr
165 170 175
Val Leu Val Ala Ala His Gly Asn Ser Leu Arg Ala Leu Val Lys His
180 185 190
Leu Asp Asn Ile Ser Asp Glu Asp Ile Ala Ala Leu Asn Ile Pro Thr
195 200 205
Gly Ile Pro Leu Val Tyr Glu Leu Asp Ala Asp Gly Lys Val Val Asn
210 215 220
Pro Gly Gly Thr Tyr Leu Asp Pro Glu Ala Ala Ala Ala Gly Ala Ala
225 230 235 240
Ala Val Ala Ala Gln Gly Gly Lys
245
<210> 149
<211> 1818
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1518)
<223> ген pgk
<400> 149
cgatccgcac ggctccatct tcgacgccgg cctgaccaag gtcatcggcg accaggtcaa 60
ggtcgtctcc tggtacgaca acgagtgggg ctacacctgc cagctcctgc gcctgaccga 120
gctcgtcgcc tccaagctct aggaccgcga ccccgcactt ccccggcctc cggctgagac 180
cgggggagtg cgcccctgtg gcccggggtg tgcctcggca cacgtcgggc ctcagggcta 240
tccaccccct cagatcagcc gcccctttct catctgaaga caaaagtcaa ggagacagcc 300
atg acc gtg aag act ctg cag gac ctg ctc gac gag ggc gtc gac ggc 348
Met Thr Val Lys Thr Leu Gln Asp Leu Leu Asp Glu Gly Val Asp Gly
1 5 10 15
cgc cac atc ctc gtc cgc tcc gac ttc aac gtc ccg ctc aac gac gcc 396
Arg His Ile Leu Val Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Ala
20 25 30
ggt gag atc acc gac gcg ggc cgc atc acc gcc tcc ctg ccc acc ctc 444
Gly Glu Ile Thr Asp Ala Gly Arg Ile Thr Ala Ser Leu Pro Thr Leu
35 40 45
aag gcg ctc gtc gag ggt ggc gcg aag gtc atc gtc atg gcc cac ctg 492
Lys Ala Leu Val Glu Gly Gly Ala Lys Val Ile Val Met Ala His Leu
50 55 60
ggc cgc ccg aag ggc gag gtc aac ccg aag tac tcc ctg gcc ccg gtc 540
Gly Arg Pro Lys Gly Glu Val Asn Pro Lys Tyr Ser Leu Ala Pro Val
65 70 75 80
gcc gag gcc ctc tcc gag gca ctc ggc cag tac gtc gcc ctc gcc ggc 588
Ala Glu Ala Leu Ser Glu Ala Leu Gly Gln Tyr Val Ala Leu Ala Gly
85 90 95
gac gtc acc ggt gag gac gcc cac gag cgc gcc aac ggc ctg acc gag 636
Asp Val Thr Gly Glu Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu
100 105 110
ggc gac gtc ctg ctc ctc gag aac gtg cgt ttc gac gcc cgc gag acc 684
Gly Asp Val Leu Leu Leu Glu Asn Val Arg Phe Asp Ala Arg Glu Thr
115 120 125
tcc aag gac gag gcc gag cgc ggc gcc ttc gcc gac gag ctc gcc gcc 732
Ser Lys Asp Glu Ala Glu Arg Gly Ala Phe Ala Asp Glu Leu Ala Ala
130 135 140
ctg gcc gcc gac aac ggc gcc ttc gtc tcc gac ggt ttc ggt gtc gtc 780
Leu Ala Ala Asp Asn Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val
145 150 155 160
cac cgt gcg cag gcc tcc gtc tac gac gtc gcc aag cgt ctc ccg gcc 828
His Arg Ala Gln Ala Ser Val Tyr Asp Val Ala Lys Arg Leu Pro Ala
165 170 175
tac gcc ggc aac ctg gtg gag aag gag atc tcc gtc ctg gag aag acc 876
Tyr Ala Gly Asn Leu Val Glu Lys Glu Ile Ser Val Leu Glu Lys Thr
180 185 190
gcc acc aac ccg gag tcc ccg tac gtc gtc gtc ctc ggc ggc tcc aag 924
Ala Thr Asn Pro Glu Ser Pro Tyr Val Val Val Leu Gly Gly Ser Lys
195 200 205
gtc tcc gac aag ctg ggc gtc atc gag gct ctg gcc ggc aag gcc gac 972
Val Ser Asp Lys Leu Gly Val Ile Glu Ala Leu Ala Gly Lys Ala Asp
210 215 220
aag atc atc atc ggt ggc ggc atg tgc tac acc ttc ctc aac gcc cag 1020
Lys Ile Ile Ile Gly Gly Gly Met Cys Tyr Thr Phe Leu Asn Ala Gln
225 230 235 240
ggc tac aac acc cag aag tcc ctg ctg cag gag gag atg atc gac acc 1068
Gly Tyr Asn Thr Gln Lys Ser Leu Leu Gln Glu Glu Met Ile Asp Thr
245 250 255
tgc aag gac ctc ctc gag cgt ttc ggc gac aag atc gtc ctc ccg gtg 1116
Cys Lys Asp Leu Leu Glu Arg Phe Gly Asp Lys Ile Val Leu Pro Val
260 265 270
gac ctc gtg gcc gcc gcc gag ttc tcc gcc gac tcc gag cac aag gtc 1164
Asp Leu Val Ala Ala Ala Glu Phe Ser Ala Asp Ser Glu His Lys Val
275 280 285
gtc gcc ctc gac gag atc ccg gag ggc tgg ctg tcc ccg gac atc ggc 1212
Val Ala Leu Asp Glu Ile Pro Glu Gly Trp Leu Ser Pro Asp Ile Gly
290 295 300
ccg gag tcc gtc aag aag ttc gcc gac gtc ctc gcc acc tcc aag acc 1260
Pro Glu Ser Val Lys Lys Phe Ala Asp Val Leu Ala Thr Ser Lys Thr
305 310 315 320
gtc ttc tgg aac ggc ccg atg ggt gtc ttc gag atg gca ccg ttc tcc 1308
Val Phe Trp Asn Gly Pro Met Gly Val Phe Glu Met Ala Pro Phe Ser
325 330 335
aag ggc acc gag ggc gtc gcc aag gcc atc atc gac gcg acc gcg aac 1356
Lys Gly Thr Glu Gly Val Ala Lys Ala Ile Ile Asp Ala Thr Ala Asn
340 345 350
aac ggt tcc ttc tcc gtc gtc ggt ggc ggc gac tcc gca gcc tcc gtc 1404
Asn Gly Ser Phe Ser Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val
355 360 365
cgc gtg ctc ggc ctg gac gag gac ggc ttc tcc cac atc tcc acc ggc 1452
Arg Val Leu Gly Leu Asp Glu Asp Gly Phe Ser His Ile Ser Thr Gly
370 375 380
ggc ggc gcc tcc ctc gag ttc ctc gag ggc aag gag ctc ccg ggc gtt 1500
Gly Gly Ala Ser Leu Glu Phe Leu Glu Gly Lys Glu Leu Pro Gly Val
385 390 395 400
tcc gtc ctg gac cgc taa gcacttacca cccactctga aaggagtgcc 1548
Ser Val Leu Asp Arg
405
ccacccatgg cacgcacccc cctcatcgcc ggcaactgga agatgaacct ggaccaccac 1608
caggccatcg ccaccgtgca gaagctcgac ttcgccctgc cgaaggagta ctacgagaag 1668
gtcgacgtcg ccgtcaccgt cccgttcacc gacctccgtt ccgtgcagac gctcgtcgac 1728
ggggacaagc tcaaggtcac ctacggtgcc caggacgtct cccggcacga gtccggcgcg 1788
tacaccggtg aggtctccgc tcagatgctc 1818
<210> 150
<211> 405
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 150
Met Thr Val Lys Thr Leu Gln Asp Leu Leu Asp Glu Gly Val Asp Gly
1 5 10 15
Arg His Ile Leu Val Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Ala
20 25 30
Gly Glu Ile Thr Asp Ala Gly Arg Ile Thr Ala Ser Leu Pro Thr Leu
35 40 45
Lys Ala Leu Val Glu Gly Gly Ala Lys Val Ile Val Met Ala His Leu
50 55 60
Gly Arg Pro Lys Gly Glu Val Asn Pro Lys Tyr Ser Leu Ala Pro Val
65 70 75 80
Ala Glu Ala Leu Ser Glu Ala Leu Gly Gln Tyr Val Ala Leu Ala Gly
85 90 95
Asp Val Thr Gly Glu Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu
100 105 110
Gly Asp Val Leu Leu Leu Glu Asn Val Arg Phe Asp Ala Arg Glu Thr
115 120 125
Ser Lys Asp Glu Ala Glu Arg Gly Ala Phe Ala Asp Glu Leu Ala Ala
130 135 140
Leu Ala Ala Asp Asn Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val
145 150 155 160
His Arg Ala Gln Ala Ser Val Tyr Asp Val Ala Lys Arg Leu Pro Ala
165 170 175
Tyr Ala Gly Asn Leu Val Glu Lys Glu Ile Ser Val Leu Glu Lys Thr
180 185 190
Ala Thr Asn Pro Glu Ser Pro Tyr Val Val Val Leu Gly Gly Ser Lys
195 200 205
Val Ser Asp Lys Leu Gly Val Ile Glu Ala Leu Ala Gly Lys Ala Asp
210 215 220
Lys Ile Ile Ile Gly Gly Gly Met Cys Tyr Thr Phe Leu Asn Ala Gln
225 230 235 240
Gly Tyr Asn Thr Gln Lys Ser Leu Leu Gln Glu Glu Met Ile Asp Thr
245 250 255
Cys Lys Asp Leu Leu Glu Arg Phe Gly Asp Lys Ile Val Leu Pro Val
260 265 270
Asp Leu Val Ala Ala Ala Glu Phe Ser Ala Asp Ser Glu His Lys Val
275 280 285
Val Ala Leu Asp Glu Ile Pro Glu Gly Trp Leu Ser Pro Asp Ile Gly
290 295 300
Pro Glu Ser Val Lys Lys Phe Ala Asp Val Leu Ala Thr Ser Lys Thr
305 310 315 320
Val Phe Trp Asn Gly Pro Met Gly Val Phe Glu Met Ala Pro Phe Ser
325 330 335
Lys Gly Thr Glu Gly Val Ala Lys Ala Ile Ile Asp Ala Thr Ala Asn
340 345 350
Asn Gly Ser Phe Ser Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val
355 360 365
Arg Val Leu Gly Leu Asp Glu Asp Gly Phe Ser His Ile Ser Thr Gly
370 375 380
Gly Gly Ala Ser Leu Glu Phe Leu Glu Gly Lys Glu Leu Pro Gly Val
385 390 395 400
Ser Val Leu Asp Arg
405
<210> 151
<211> 2058
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1758)
<223> ген глицеральдегид_3_фосфатдегидрогеназы 1
<400> 151
gcggcgggcg ggcgcagcgt ggcgagccgg gccttcagcg gccgcgtcgc ccgcggactg 60
gaaacggact tcacccggaa caacccggac atcccgccga tctaccccta cctcgtgtcg 120
ctgcaggccg cggaacggcg ggaacgcccg cgggaactgg cctactgcct cgtcggggtg 180
ggcgtcgaga agctcggggg tggccccgcg gcggggattc tggctcgact cgcgcctgaa 240
caggcaattt cacgctaaag tcgtggggac gtttcccttt actttcggag gtaaaaagca 300
gtg acc gcc cac gcg cag cct gag cac aac aac tgg aac gag aag atc 348
Val Thr Ala His Ala Gln Pro Glu His Asn Asn Trp Asn Glu Lys Ile
1 5 10 15
gcc ctc gct cag gag atg ctg ccg ctg atc ggc cag ctc cac cgc aac 396
Ala Leu Ala Gln Glu Met Leu Pro Leu Ile Gly Gln Leu His Arg Asn
20 25 30
aac gat gtc gtc gtc tcc atc ttc gga cga ctg ctt gtc ggc gcg acg 444
Asn Asp Val Val Val Ser Ile Phe Gly Arg Leu Leu Val Gly Ala Thr
35 40 45
gac atc gac atc atc aag gcg cac cgt tac gcg cgc cgc gtc gcc gac 492
Asp Ile Asp Ile Ile Lys Ala His Arg Tyr Ala Arg Arg Val Ala Asp
50 55 60
cgt gag atg aag ctc gat cag acc ctc ccg gtc ctg cgc gag ctc gtc 540
Arg Glu Met Lys Leu Asp Gln Thr Leu Pro Val Leu Arg Glu Leu Val
65 70 75 80
gac atg gac ctc ggc acc gcc tcc atc gac ctg ggc cgc ctg gcc acc 588
Asp Met Asp Leu Gly Thr Ala Ser Ile Asp Leu Gly Arg Leu Ala Thr
85 90 95
ggc ttc gag aag tcc ggc ggc acc gac ctg cgt gcc tac ctg gac gag 636
Gly Phe Glu Lys Ser Gly Gly Thr Asp Leu Arg Ala Tyr Leu Asp Glu
100 105 110
cag ctc gcc gac atc gtc ggc aag cgc tcc gag acc gag ccg cgc gac 684
Gln Leu Ala Asp Ile Val Gly Lys Arg Ser Glu Thr Glu Pro Arg Asp
115 120 125
gtc gtc ctc tac ggc ttc ggc cgc atc ggc cgc ctc ctg gcc cgc atc 732
Val Val Leu Tyr Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile
130 135 140
ctc atc tcc cgt gag gcc acc tac ggc ggc gtc cgc ctc cgc gcg gtg 780
Leu Ile Ser Arg Glu Ala Thr Tyr Gly Gly Val Arg Leu Arg Ala Val
145 150 155 160
gtg gta cgc aag aac tcc gag gac gac atc gtc aag cgt gcc tcc ctc 828
Val Val Arg Lys Asn Ser Glu Asp Asp Ile Val Lys Arg Ala Ser Leu
165 170 175
ctg cgc cgt gac tcc gtg cac ggc gcc ttc gac ggc acg atc acc gtc 876
Leu Arg Arg Asp Ser Val His Gly Ala Phe Asp Gly Thr Ile Thr Val
180 185 190
gac aag gag aac aac gtc atc tgg gcc aac ggc acc aag atc cag atc 924
Asp Lys Glu Asn Asn Val Ile Trp Ala Asn Gly Thr Lys Ile Gln Ile
195 200 205
atc tac tcc aac tcc ccg gcc gag gtc gac tac acc gcc tac ggc atc 972
Ile Tyr Ser Asn Ser Pro Ala Glu Val Asp Tyr Thr Ala Tyr Gly Ile
210 215 220
aat gac gcc atc gtg gtg gac aac acc ggc atc tgg cgc gac cgc gag 1020
Asn Asp Ala Ile Val Val Asp Asn Thr Gly Ile Trp Arg Asp Arg Glu
225 230 235 240
gga ctg tcc cag cac ctg cag tcc aag ggc gtc tcc cgc gtc gtc ctc 1068
Gly Leu Ser Gln His Leu Gln Ser Lys Gly Val Ser Arg Val Val Leu
245 250 255
acc gcc ccg ggc aag ggc gac ctg ccg aac atc gtc tac ggc atc aac 1116
Thr Ala Pro Gly Lys Gly Asp Leu Pro Asn Ile Val Tyr Gly Ile Asn
260 265 270
gac gac atg atc acc gac gac gag cag atc atc acc gcc gcc tcc tgc 1164
Asp Asp Met Ile Thr Asp Asp Glu Gln Ile Ile Thr Ala Ala Ser Cys
275 280 285
acc acc aac ggc atc acc ccg gtc ctg aag gtc atc aac gac cgc tac 1212
Thr Thr Asn Gly Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr
290 295 300
ggc gtc gag cac ggt cac gtc gag acc gtc cac tcc ttc acc aac gac 1260
Gly Val Glu His Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp
305 310 315 320
cag aac ctc atc gac aac ttc cac aag ggc acc cgc cgt ggc cgt gcc 1308
Gln Asn Leu Ile Asp Asn Phe His Lys Gly Thr Arg Arg Gly Arg Ala
325 330 335
gcc tcc ctc aac atg gtc ctc acc gag acc ggc gcc gcc aag gcc gtc 1356
Ala Ser Leu Asn Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val
340 345 350
tcc aag gcc ctg ccg gag ttc gag ggc aag ctc acc ggc aac gcc atc 1404
Ser Lys Ala Leu Pro Glu Phe Glu Gly Lys Leu Thr Gly Asn Ala Ile
355 360 365
cgc gtc ccc acc ccg aac gtc tcc atg gcg gtg ctc aac ctc acc ctg 1452
Arg Val Pro Thr Pro Asn Val Ser Met Ala Val Leu Asn Leu Thr Leu
370 375 380
gag aag gag gtc gac cgc gag gag gtc aac aac ttc ctg cgc aag gtc 1500
Glu Lys Glu Val Asp Arg Glu Glu Val Asn Asn Phe Leu Arg Lys Val
385 390 395 400
tcc ctg cac tcc gac ctg cgc cag cag atc tcc tac atc aac tcc ccg 1548
Ser Leu His Ser Asp Leu Arg Gln Gln Ile Ser Tyr Ile Asn Ser Pro
405 410 415
gag gtc gtg tcc acc gac ttc gtc ggc agc acc cac gcc ggc atc gtc 1596
Glu Val Val Ser Thr Asp Phe Val Gly Ser Thr His Ala Gly Ile Val
420 425 430
gac ggc ctg gcc acc atc gcc tcc ggc aag cac ctc gtg ctc tac gtc 1644
Asp Gly Leu Ala Thr Ile Ala Ser Gly Lys His Leu Val Leu Tyr Val
435 440 445
tgg tac gac aac gag ttc ggc tac tcc aac cag gtc atc cgc atc gtc 1692
Trp Tyr Asp Asn Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val
450 455 460
gag gag gtc gcc ggc gcc cgc ccg gag atc tac ccg cgt cgc ctg gag 1740
Glu Glu Val Ala Gly Ala Arg Pro Glu Ile Tyr Pro Arg Arg Leu Glu
465 470 475 480
gcc tct gag ctg aag tag cttttccagg atcagcttca agctgccgca 1788
Ala Ser Glu Leu Lys
485
gcgacaggcc cggttctcca cgtggagaac cgggcctgtc gcattcagga ggggcggcag 1848
cggcacaggc agatgaagaa tcgtcgtcgg tgtgtccgct cactgcgcct ggtgttaggc 1908
ccgtgctcta cacccgcccg ctgccggtta cggcaatcat ggccaggagc aggtgctgac 1968
cggtctgaca ggcgcctacg ctcacccagt caactgctga ctgggacatt gacttgagga 2028
tgcgtgcttc ccggcgtatg tggtcccttg 2058
<210> 152
<211> 485
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 152
Val Thr Ala His Ala Gln Pro Glu His Asn Asn Trp Asn Glu Lys Ile
1 5 10 15
Ala Leu Ala Gln Glu Met Leu Pro Leu Ile Gly Gln Leu His Arg Asn
20 25 30
Asn Asp Val Val Val Ser Ile Phe Gly Arg Leu Leu Val Gly Ala Thr
35 40 45
Asp Ile Asp Ile Ile Lys Ala His Arg Tyr Ala Arg Arg Val Ala Asp
50 55 60
Arg Glu Met Lys Leu Asp Gln Thr Leu Pro Val Leu Arg Glu Leu Val
65 70 75 80
Asp Met Asp Leu Gly Thr Ala Ser Ile Asp Leu Gly Arg Leu Ala Thr
85 90 95
Gly Phe Glu Lys Ser Gly Gly Thr Asp Leu Arg Ala Tyr Leu Asp Glu
100 105 110
Gln Leu Ala Asp Ile Val Gly Lys Arg Ser Glu Thr Glu Pro Arg Asp
115 120 125
Val Val Leu Tyr Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile
130 135 140
Leu Ile Ser Arg Glu Ala Thr Tyr Gly Gly Val Arg Leu Arg Ala Val
145 150 155 160
Val Val Arg Lys Asn Ser Glu Asp Asp Ile Val Lys Arg Ala Ser Leu
165 170 175
Leu Arg Arg Asp Ser Val His Gly Ala Phe Asp Gly Thr Ile Thr Val
180 185 190
Asp Lys Glu Asn Asn Val Ile Trp Ala Asn Gly Thr Lys Ile Gln Ile
195 200 205
Ile Tyr Ser Asn Ser Pro Ala Glu Val Asp Tyr Thr Ala Tyr Gly Ile
210 215 220
Asn Asp Ala Ile Val Val Asp Asn Thr Gly Ile Trp Arg Asp Arg Glu
225 230 235 240
Gly Leu Ser Gln His Leu Gln Ser Lys Gly Val Ser Arg Val Val Leu
245 250 255
Thr Ala Pro Gly Lys Gly Asp Leu Pro Asn Ile Val Tyr Gly Ile Asn
260 265 270
Asp Asp Met Ile Thr Asp Asp Glu Gln Ile Ile Thr Ala Ala Ser Cys
275 280 285
Thr Thr Asn Gly Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr
290 295 300
Gly Val Glu His Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp
305 310 315 320
Gln Asn Leu Ile Asp Asn Phe His Lys Gly Thr Arg Arg Gly Arg Ala
325 330 335
Ala Ser Leu Asn Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val
340 345 350
Ser Lys Ala Leu Pro Glu Phe Glu Gly Lys Leu Thr Gly Asn Ala Ile
355 360 365
Arg Val Pro Thr Pro Asn Val Ser Met Ala Val Leu Asn Leu Thr Leu
370 375 380
Glu Lys Glu Val Asp Arg Glu Glu Val Asn Asn Phe Leu Arg Lys Val
385 390 395 400
Ser Leu His Ser Asp Leu Arg Gln Gln Ile Ser Tyr Ile Asn Ser Pro
405 410 415
Glu Val Val Ser Thr Asp Phe Val Gly Ser Thr His Ala Gly Ile Val
420 425 430
Asp Gly Leu Ala Thr Ile Ala Ser Gly Lys His Leu Val Leu Tyr Val
435 440 445
Trp Tyr Asp Asn Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val
450 455 460
Glu Glu Val Ala Gly Ala Arg Pro Glu Ile Tyr Pro Arg Arg Leu Glu
465 470 475 480
Ala Ser Glu Leu Lys
485
<210> 153
<211> 1605
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1305)
<223> ген глицеральдегид_3_фосфатдегидрогеназы 2
<400> 153
gctccattcg ggggtgaatc gggcatgtgt gaccgatatt attcgccggt gtccggagga 60
ccgcccggtg cccggccgcc tgcgaccccc cgttgacaac gactttcggg aagtggggcg 120
gaccggaatg tgagggcaaa cataatcgga aatccgcgga tgtctgaaaa tgtctgattc 180
ttcgctgcac agcggggtaa gtgccgctaa actgtcgctt gatgggccgt gaacgccagt 240
cactacgacg gacagtgcgg tcacatccgg acacgtgtac tcaaacccga ggagaaaatt 300
gtg acc atc cgc gtt ggc atc aac ggc ttc ggc cgt atc ggc cgt aac 348
Val Thr Ile Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn
1 5 10 15
ttc tac cgc gca gtc ctg gag cgc agc gag gcc atc gag atc gtc gcc 396
Phe Tyr Arg Ala Val Leu Glu Arg Ser Glu Ala Ile Glu Ile Val Ala
20 25 30
gtc aac gac ctc acc gac aac aag acg ctg tcg acc ctc ctg aag tac 444
Val Asn Asp Leu Thr Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Tyr
35 40 45
gac tcc atc ctc ggc aag ctg ccg ggc gag gtc tcg ttc gac gac gac 492
Asp Ser Ile Leu Gly Lys Leu Pro Gly Glu Val Ser Phe Asp Asp Asp
50 55 60
tcc atc acc tac gac ggc aag acc atc gcc gtc tcc gct gag cgc gac 540
Ser Ile Thr Tyr Asp Gly Lys Thr Ile Ala Val Ser Ala Glu Arg Asp
65 70 75 80
ccg aag aac ctg gac tgg gcc gcc cac aac gtg gac atc gtc atc gag 588
Pro Lys Asn Leu Asp Trp Ala Ala His Asn Val Asp Ile Val Ile Glu
85 90 95
tcc acc ggc ttc ttc acc gac gcc gag gca gcc aag gcc cac atc gag 636
Ser Thr Gly Phe Phe Thr Asp Ala Glu Ala Ala Lys Ala His Ile Glu
100 105 110
gcc ggc gcc aag aag gtc atc atc tcc gcc ccg gcc tcc aac gag gac 684
Ala Gly Ala Lys Lys Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp
115 120 125
gcg acc ttc gtc tac ggc gtc aac cac cag gac tac gac ccg gag aac 732
Ala Thr Phe Val Tyr Gly Val Asn His Gln Asp Tyr Asp Pro Glu Asn
130 135 140
cac aac gtc atc tcc gcc gca tcc tgc acc acc aac tgc ctg gcc ccg 780
His Asn Val Ile Ser Ala Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro
145 150 155 160
atg gcc aag gtc ctc aac gac aag ctc ggc atc gag cgc ggc ctg atg 828
Met Ala Lys Val Leu Asn Asp Lys Leu Gly Ile Glu Arg Gly Leu Met
165 170 175
acc acc atc cac gcc tac acc ggt gac cag cgt ctg cac gac gcc ccg 876
Thr Thr Ile His Ala Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro
180 185 190
cac aag gac ctg cgc cgc gcc cgc gcc gcc gcc gtc aac atc gtg ccc 924
His Lys Asp Leu Arg Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro
195 200 205
acc tcc acc ggt gcc gcc aag gcc gtc tcc ctg gtc ctg ccg gag ctg 972
Thr Ser Thr Gly Ala Ala Lys Ala Val Ser Leu Val Leu Pro Glu Leu
210 215 220
aag ggc aag ctg gac ggc tac gca ctg cgc gtc ccg acc atc acc ggt 1020
Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val Pro Thr Ile Thr Gly
225 230 235 240
tcc gcc acc gac ctc acc ttc acc gcc tcc cgc gag acc tct gtc gag 1068
Ser Ala Thr Asp Leu Thr Phe Thr Ala Ser Arg Glu Thr Ser Val Glu
245 250 255
gag gtc aac gcc ttc atc aag gag gcc gcg cag ggc gag ttc ggt gag 1116
Glu Val Asn Ala Phe Ile Lys Glu Ala Ala Gln Gly Glu Phe Gly Glu
260 265 270
acc ctg gcc tac acc gag gat ccg atc gtc tcc acc gac atc gtc acc 1164
Thr Leu Ala Tyr Thr Glu Asp Pro Ile Val Ser Thr Asp Ile Val Thr
275 280 285
gat ccg cac ggc tcc atc ttc gac gcc ggc ctg acc aag gtc atc ggc 1212
Asp Pro His Gly Ser Ile Phe Asp Ala Gly Leu Thr Lys Val Ile Gly
290 295 300
gac cag gtc aag gtc gtc tcc tgg tac gac aac gag tgg ggc tac acc 1260
Asp Gln Val Lys Val Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr
305 310 315 320
tgc cag ctc ctg cgc ctg acc gag ctc gtc gcc tcc aag ctc tag 1305
Cys Gln Leu Leu Arg Leu Thr Glu Leu Val Ala Ser Lys Leu
325 330
gaccgcgacc ccgcacttcc ccggcctccg gctgagaccg ggggagtgcg cccctgtggc 1365
ccggggtgtg cctcggcaca cgtcgggcct cagggctatc caccccctca gatcagccgc 1425
ccctttctca tctgaagaca aaagtcaagg agacagccat gaccgtgaag actctgcagg 1485
acctgctcga cgagggcgtc gacggccgcc acatcctcgt ccgctccgac ttcaacgtcc 1545
cgctcaacga cgccggtgag atcaccgacg cgggccgcat caccgcctcc ctgcccaccc 1605
<210> 154
<211> 334
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 154
Val Thr Ile Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn
1 5 10 15
Phe Tyr Arg Ala Val Leu Glu Arg Ser Glu Ala Ile Glu Ile Val Ala
20 25 30
Val Asn Asp Leu Thr Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Tyr
35 40 45
Asp Ser Ile Leu Gly Lys Leu Pro Gly Glu Val Ser Phe Asp Asp Asp
50 55 60
Ser Ile Thr Tyr Asp Gly Lys Thr Ile Ala Val Ser Ala Glu Arg Asp
65 70 75 80
Pro Lys Asn Leu Asp Trp Ala Ala His Asn Val Asp Ile Val Ile Glu
85 90 95
Ser Thr Gly Phe Phe Thr Asp Ala Glu Ala Ala Lys Ala His Ile Glu
100 105 110
Ala Gly Ala Lys Lys Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp
115 120 125
Ala Thr Phe Val Tyr Gly Val Asn His Gln Asp Tyr Asp Pro Glu Asn
130 135 140
His Asn Val Ile Ser Ala Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro
145 150 155 160
Met Ala Lys Val Leu Asn Asp Lys Leu Gly Ile Glu Arg Gly Leu Met
165 170 175
Thr Thr Ile His Ala Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro
180 185 190
His Lys Asp Leu Arg Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro
195 200 205
Thr Ser Thr Gly Ala Ala Lys Ala Val Ser Leu Val Leu Pro Glu Leu
210 215 220
Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val Pro Thr Ile Thr Gly
225 230 235 240
Ser Ala Thr Asp Leu Thr Phe Thr Ala Ser Arg Glu Thr Ser Val Glu
245 250 255
Glu Val Asn Ala Phe Ile Lys Glu Ala Ala Gln Gly Glu Phe Gly Glu
260 265 270
Thr Leu Ala Tyr Thr Glu Asp Pro Ile Val Ser Thr Asp Ile Val Thr
275 280 285
Asp Pro His Gly Ser Ile Phe Asp Ala Gly Leu Thr Lys Val Ile Gly
290 295 300
Asp Gln Val Lys Val Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr
305 310 315 320
Cys Gln Leu Leu Arg Leu Thr Glu Leu Val Ala Ser Lys Leu
325 330
<210> 155
<211> 1380
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1080)
<223> ген tpiA
<400> 155
aagaccgtct tctggaacgg cccgatgggt gtcttcgaga tggcaccgtt ctccaagggc 60
accgagggcg tcgccaaggc catcatcgac gcgaccgcga acaacggttc cttctccgtc 120
gtcggtggcg gcgactccgc agcctccgtc cgcgtgctcg gcctggacga ggacggcttc 180
tcccacatct ccaccggcgg cggcgcctcc ctcgagttcc tcgagggcaa ggagctcccg 240
ggcgtttccg tcctggaccg ctaagcactt accacccact ctgaaaggag tgccccaccc 300
atg gca cgc acc ccc ctc atc gcc ggc aac tgg aag atg aac ctg gac 348
Met Ala Arg Thr Pro Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp
1 5 10 15
cac cac cag gcc atc gcc acc gtg cag aag ctc gac ttc gcc ctg ccg 396
His His Gln Ala Ile Ala Thr Val Gln Lys Leu Asp Phe Ala Leu Pro
20 25 30
aag gag tac tac gag aag gtc gac gtc gcc gtc acc gtc ccg ttc acc 444
Lys Glu Tyr Tyr Glu Lys Val Asp Val Ala Val Thr Val Pro Phe Thr
35 40 45
gac ctc cgt tcc gtg cag acg ctc gtc gac ggg gac aag ctc aag gtc 492
Asp Leu Arg Ser Val Gln Thr Leu Val Asp Gly Asp Lys Leu Lys Val
50 55 60
acc tac ggt gcc cag gac gtc tcc cgg cac gag tcc ggc gcg tac acc 540
Thr Tyr Gly Ala Gln Asp Val Ser Arg His Glu Ser Gly Ala Tyr Thr
65 70 75 80
ggt gag gtc tcc gct cag atg ctc gcc aag ctc ggc tgc acc tgg gtc 588
Gly Glu Val Ser Ala Gln Met Leu Ala Lys Leu Gly Cys Thr Trp Val
85 90 95
gtc gtc ggc cac tcc gag cgc cgc gag tac cac aac gag tcc gac gag 636
Val Val Gly His Ser Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu
100 105 110
ctg gtc gcc gcg aag gcc gct gcc gcc ctc cgc cac ggc atg agc ccg 684
Leu Val Ala Ala Lys Ala Ala Ala Ala Leu Arg His Gly Met Ser Pro
115 120 125
atc gtg tgc gtc ggc gag ccg ctg gag atc cgc gag gcc ggc acc cac 732
Ile Val Cys Val Gly Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His
130 135 140
gtc gac tac gtc gtc gag cag acc cgt gcc tcc ctg gcc ggc ctg tcc 780
Val Asp Tyr Val Val Glu Gln Thr Arg Ala Ser Leu Ala Gly Leu Ser
145 150 155 160
gcc gag gac ctg tcc aac acc gtc atc gcc tac gag ccc gtg tgg gcc 828
Ala Glu Asp Leu Ser Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala
165 170 175
atc ggc acc ggc aag gtc gcc tcc gcg gcc gat gcc cag gag gtc tgt 876
Ile Gly Thr Gly Lys Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys
180 185 190
gcg gcg atc cgc ggt ctc gtc cgc gag ctg gcc ggt gac gag gtc gcc 924
Ala Ala Ile Arg Gly Leu Val Arg Glu Leu Ala Gly Asp Glu Val Ala
195 200 205
gac ggc atc cgc atc ctc tac ggc ggt tcc gtc aag acc gac tcg gtc 972
Asp Gly Ile Arg Ile Leu Tyr Gly Gly Ser Val Lys Thr Asp Ser Val
210 215 220
gcc gag atc gtc ggt cag ccc gac gtc gac ggt ggt ctc gtc ggt ggc 1020
Ala Glu Ile Val Gly Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly
225 230 235 240
gcc tcg ctc gac ggc gag gag ttc gcc aag ctg gcg gcc gcc gcg gcg 1068
Ala Ser Leu Asp Gly Glu Glu Phe Ala Lys Leu Ala Ala Ala Ala Ala
245 250 255
tcc gtc gtc tag catcacctca tccaccggcc ccgggcactg aacattcagt 1120
Ser Val Val
gcccggggcc ggtgtccttt ctccggggaa tatggtgcgc aatccccacg cacggccgtc 1180
atatgcgtca tgatggggag gcgaaccgca tccccgcctt cttcgagtac gaacagggag 1240
acactcaatg accgtccgcg atcatctgca ggaagacatc cgctacctcg gccgtatcct 1300
cggccaggtc atcgcggaac aggagggcga ggacgtgttc aacctcgtcg agcacgcccg 1360
ccagcaggcc ttcgaggtgg 1380
<210> 156
<211> 259
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 156
Met Ala Arg Thr Pro Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp
1 5 10 15
His His Gln Ala Ile Ala Thr Val Gln Lys Leu Asp Phe Ala Leu Pro
20 25 30
Lys Glu Tyr Tyr Glu Lys Val Asp Val Ala Val Thr Val Pro Phe Thr
35 40 45
Asp Leu Arg Ser Val Gln Thr Leu Val Asp Gly Asp Lys Leu Lys Val
50 55 60
Thr Tyr Gly Ala Gln Asp Val Ser Arg His Glu Ser Gly Ala Tyr Thr
65 70 75 80
Gly Glu Val Ser Ala Gln Met Leu Ala Lys Leu Gly Cys Thr Trp Val
85 90 95
Val Val Gly His Ser Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu
100 105 110
Leu Val Ala Ala Lys Ala Ala Ala Ala Leu Arg His Gly Met Ser Pro
115 120 125
Ile Val Cys Val Gly Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His
130 135 140
Val Asp Tyr Val Val Glu Gln Thr Arg Ala Ser Leu Ala Gly Leu Ser
145 150 155 160
Ala Glu Asp Leu Ser Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala
165 170 175
Ile Gly Thr Gly Lys Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys
180 185 190
Ala Ala Ile Arg Gly Leu Val Arg Glu Leu Ala Gly Asp Glu Val Ala
195 200 205
Asp Gly Ile Arg Ile Leu Tyr Gly Gly Ser Val Lys Thr Asp Ser Val
210 215 220
Ala Glu Ile Val Gly Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly
225 230 235 240
Ala Ser Leu Asp Gly Glu Glu Phe Ala Lys Leu Ala Ala Ala Ala Ala
245 250 255
Ser Val Val
<210> 157
<211> 1632
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1332)
<223> ген фруктозобисфосфатальдолазы
<400> 157
ccgactggac cgccgacgcc cgcctgccgc acaacttcgg cctggcgccg tcgtcactgc 60
cggaggccct cggcgtcctg cgtatcgacg aacgcgatct ctccgtccag ctctccggct 120
ggatgctgct ggaggccgcc gcccgggtgt ccacctacgc ggagacccag cggtcctgat 180
ccccgcgcgc ggggcggggg gggaggtgac gcgggttttc cgccctctgt tgcgagcgcg 240
ggaggccaaa gggaggcata ctggtatacg gacctgtttg ctaacccgga ggatcaccct 300
atg cct atc gcg act ccc gaa atc tac aac gag atg ctc gac agg gcc 348
Met Pro Ile Ala Thr Pro Glu Ile Tyr Asn Glu Met Leu Asp Arg Ala
1 5 10 15
aag gag ggc ggc tac gcc ttc ccg gcc atc aac tgc acc tcc tcg gag 396
Lys Glu Gly Gly Tyr Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu
20 25 30
acc atc aac gcc gct ctc cgc ggc ttc gcc gag gcc gag tcc gac ggc 444
Thr Ile Asn Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly
35 40 45
atc atc cag ttc tcc acc ggt ggc gcc gaa ttc ggc tcc ggt ctg tcc 492
Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ser
50 55 60
gtg aag aac aag gtc gcc ggc gcc gtc gcc ctg gcc tcc ttc gcg cat 540
Val Lys Asn Lys Val Ala Gly Ala Val Ala Leu Ala Ser Phe Ala His
65 70 75 80
gag gcc gcc aag cac tac ggc gtc aac gtc gcc ctg cac acc gac cac 588
Glu Ala Ala Lys His Tyr Gly Val Asn Val Ala Leu His Thr Asp His
85 90 95
tgc cag aag gag gtg ctc gac gag ttc gtg cgc ccg ctg ctg gcc atc 636
Cys Gln Lys Glu Val Leu Asp Glu Phe Val Arg Pro Leu Leu Ala Ile
100 105 110
tcc cag gag cgc gtc gac gcc ggt gag ctc ccg ctc ttc cag tcc cac 684
Ser Gln Glu Arg Val Asp Ala Gly Glu Leu Pro Leu Phe Gln Ser His
115 120 125
atg tgg gac ggt tcc gct gtc ccg atc gac gag aac ctg gag atc gcg 732
Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala
130 135 140
aag gag ctg ctg gag aag tcc cgc aag gcg aac att atc ctc gag atc 780
Lys Glu Leu Leu Glu Lys Ser Arg Lys Ala Asn Ile Ile Leu Glu Ile
145 150 155 160
gag atc ggt gtc gtc ggc ggc gag gag gac ggt gtc gag gcc aag gcc 828
Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala
165 170 175
ggc gcc aac ctc tac acc acc gag gag gac ttc gag aag acc atc gac 876
Gly Ala Asn Leu Tyr Thr Thr Glu Glu Asp Phe Glu Lys Thr Ile Asp
180 185 190
gcc atc ggc acc ggt gag aac ggc cgc tac ctg ctg gcc gcg acc ttc 924
Ala Ile Gly Thr Gly Glu Asn Gly Arg Tyr Leu Leu Ala Ala Thr Phe
195 200 205
ggc aac gtc cac ggc gtg tac aag ccg ggc aac gtg aag ctg cgt ccg 972
Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro
210 215 220
gag gtc ctg ctc gag ggc cag aag gtg gcc cgc cgc aag ctg ggt ctg 1020
Glu Val Leu Leu Glu Gly Gln Lys Val Ala Arg Arg Lys Leu Gly Leu
225 230 235 240
ggc gac gac gcc ctg ccg ttc gac ttc gtc ttc cac ggt ggc tcc ggc 1068
Gly Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly
245 250 255
tcc gag aag gag aag atc gag gag gcg ctg cgt tac ggc acc atc aag 1116
Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Arg Tyr Gly Thr Ile Lys
260 265 270
atg aac gtc gac acg gac acc cag tac gcg ttc acc cgt ccg atc gtc 1164
Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val
275 280 285
acg cac atg ttc cag aac tac gac ggc gtc ctc aag atc gac ggc gag 1212
Thr His Met Phe Gln Asn Tyr Asp Gly Val Leu Lys Ile Asp Gly Glu
290 295 300
gtg ggc aac aag aag gcc tac gac ccg cgt tcc tac ctg aag aag gcc 1260
Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Leu Lys Lys Ala
305 310 315 320
gag cag tcg atg gct gag cgc gtc atc gag gcc tgc cag gac ctg cac 1308
Glu Gln Ser Met Ala Glu Arg Val Ile Glu Ala Cys Gln Asp Leu His
325 330 335
tcc gtg ggc aag acc gtc aag tag tcttctccac ccgaggaaag ccccgccctg 1362
Ser Val Gly Lys Thr Val Lys
340
tgcggggctt tctgcgctta gtatcggtgc atggccaaac tgagaatcgg caccctggaa 1422
cgtctccgcc agctcgaggg ctcgttggag tcccgggcgc tgcgcgtgcg gaagcgcttc 1482
ttccggatcg tgcaggtcgc ggtggccgcg ggggtgtcgt tctgggtggc gcagtccctc 1542
atcgggcatc cggtcccgtt cttcgcgccg atcacggtga tcatcatcct ggggctcacc 1602
ggcggcgacc gcatccggcg ggccctggag 1632
<210> 158
<211> 343
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 158
Met Pro Ile Ala Thr Pro Glu Ile Tyr Asn Glu Met Leu Asp Arg Ala
1 5 10 15
Lys Glu Gly Gly Tyr Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu
20 25 30
Thr Ile Asn Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly
35 40 45
Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ser
50 55 60
Val Lys Asn Lys Val Ala Gly Ala Val Ala Leu Ala Ser Phe Ala His
65 70 75 80
Glu Ala Ala Lys His Tyr Gly Val Asn Val Ala Leu His Thr Asp His
85 90 95
Cys Gln Lys Glu Val Leu Asp Glu Phe Val Arg Pro Leu Leu Ala Ile
100 105 110
Ser Gln Glu Arg Val Asp Ala Gly Glu Leu Pro Leu Phe Gln Ser His
115 120 125
Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala
130 135 140
Lys Glu Leu Leu Glu Lys Ser Arg Lys Ala Asn Ile Ile Leu Glu Ile
145 150 155 160
Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala
165 170 175
Gly Ala Asn Leu Tyr Thr Thr Glu Glu Asp Phe Glu Lys Thr Ile Asp
180 185 190
Ala Ile Gly Thr Gly Glu Asn Gly Arg Tyr Leu Leu Ala Ala Thr Phe
195 200 205
Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro
210 215 220
Glu Val Leu Leu Glu Gly Gln Lys Val Ala Arg Arg Lys Leu Gly Leu
225 230 235 240
Gly Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly
245 250 255
Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Arg Tyr Gly Thr Ile Lys
260 265 270
Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val
275 280 285
Thr His Met Phe Gln Asn Tyr Asp Gly Val Leu Lys Ile Asp Gly Glu
290 295 300
Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Leu Lys Lys Ala
305 310 315 320
Glu Gln Ser Met Ala Glu Arg Val Ile Glu Ala Cys Gln Asp Leu His
325 330 335
Ser Val Gly Lys Thr Val Lys
340
<210> 159
<211> 1566
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1266)
<223> ген 1-фосфофруктокиназы
<400> 159
agaacaccac aaaatccact agttagcaac gatccagaca ggcaaccctt gacaaacgga 60
cacaatcgga caatgatgat gttgtaacac cacattaaat gtgacttaat tcatcatttt 120
cataacgtcc cgcccgggac agccccggag cgccccgatc cccgggtcgc gccccatagg 180
attgatccag ctcaggacct gttcggtcac ccgaccggac accgctccgg gcacgcgtcc 240
cccgccaccc agcggacacg ccgcaacgac acgtttttcc gaacggacag ggagcccgcc 300
gtg att ctc acc ctc acc ccc aac ccc agc atc gac gcg acc atg caa 348
Val Ile Leu Thr Leu Thr Pro Asn Pro Ser Ile Asp Ala Thr Met Gln
1 5 10 15
ctg ggg acg cgg ctg cgc cgc ggg ctc gtc cac cgt ccc tcc tcc atc 396
Leu Gly Thr Arg Leu Arg Arg Gly Leu Val His Arg Pro Ser Ser Ile
20 25 30
tcc cag gtg gcc ggc gga aaa ggc gtc aac gtc acg cac gcc ctg acc 444
Ser Gln Val Ala Gly Gly Lys Gly Val Asn Val Thr His Ala Leu Thr
35 40 45
ctc gcg gac aaa ctc agc ctc gcc ctc ttc ccg gcg gac ggc aac gac 492
Leu Ala Asp Lys Leu Ser Leu Ala Leu Phe Pro Ala Asp Gly Asn Asp
50 55 60
ccc ttc gtc gcc ctg gcc cgc cag tcc ggg atc ccc ttc cac gcc atc 540
Pro Phe Val Ala Leu Ala Arg Gln Ser Gly Ile Pro Phe His Ala Ile
65 70 75 80
ccg gtg tcc gag ggc gtc cgc atc aac acc acc ctc act gaa ccg gac 588
Pro Val Ser Glu Gly Val Arg Ile Asn Thr Thr Leu Thr Glu Pro Asp
85 90 95
ggg acc acc acc aag atc aac ggc ctc ggc gcg cag atc gag ccg ccc 636
Gly Thr Thr Thr Lys Ile Asn Gly Leu Gly Ala Gln Ile Glu Pro Pro
100 105 110
acc cgc cgc atc atc gag gag acc gtc gtc cgc ctc gcc gcg aac gcc 684
Thr Arg Arg Ile Ile Glu Glu Thr Val Val Arg Leu Ala Ala Asn Ala
115 120 125
tcc tgg ctg gtc atg gcc ggt tcc ctg ccg ccc ggg gtc ccc acc ggc 732
Ser Trp Leu Val Met Ala Gly Ser Leu Pro Pro Gly Val Pro Thr Gly
130 135 140
tgg tac acg gaa ctc atc acc acc gtc cgc gcg acg cac ccg gac ctg 780
Trp Tyr Thr Glu Leu Ile Thr Thr Val Arg Ala Thr His Pro Asp Leu
145 150 155 160
ccg gtg gcc gtc gac acg tcc gac gcc gcg atg atc gcc ctc ggc gag 828
Pro Val Ala Val Asp Thr Ser Asp Ala Ala Met Ile Ala Leu Gly Glu
165 170 175
ggc ttc gag gtc gcc gcc ccg acc ctc atc aag ccc aac ggc atg gag 876
Gly Phe Glu Val Ala Ala Pro Thr Leu Ile Lys Pro Asn Gly Met Glu
180 185 190
ctc ggg cag ctc gtc gac cgt gac ggc gag cac ctc gag gcg gcc gcc 924
Leu Gly Gln Leu Val Asp Arg Asp Gly Glu His Leu Glu Ala Ala Ala
195 200 205
gcc cgc ggc gac ttc tcc ccg gtc atc gac gcg gcc cgc gtc gtc gtc 972
Ala Arg Gly Asp Phe Ser Pro Val Ile Asp Ala Ala Arg Val Val Val
210 215 220
gac cgc ggc gtc aac gag gtc ctg gtg acg ctc ggc gtc gcc gga gcc 1020
Asp Arg Gly Val Asn Glu Val Leu Val Thr Leu Gly Val Ala Gly Ala
225 230 235 240
gcc ctc gtc acc gcg cag ggc gtc tgg ctc gcc acc cct ccc ccg gtc 1068
Ala Leu Val Thr Ala Gln Gly Val Trp Leu Ala Thr Pro Pro Pro Val
245 250 255
gac gtc atg tcc acc gtc ggc gcg ggc gac agc tcg ctc gcc ggg tac 1116
Asp Val Met Ser Thr Val Gly Ala Gly Asp Ser Ser Leu Ala Gly Tyr
260 265 270
ctc atg gcc cgc gcg gag ggc ggc agt ccc gcc gac tgc ctg gcc cgc 1164
Leu Met Ala Arg Ala Glu Gly Gly Ser Pro Ala Asp Cys Leu Ala Arg
275 280 285
gcc gtc gcc tac ggc acc gcc gcc acc ggc ctg ccc ggc acc acc att 1212
Ala Val Ala Tyr Gly Thr Ala Ala Thr Gly Leu Pro Gly Thr Thr Ile
290 295 300
cct tcc ccc gca caa ctg aat ctc gag gaa acc cat gtc cgt cat atc 1260
Pro Ser Pro Ala Gln Leu Asn Leu Glu Glu Thr His Val Arg His Ile
305 310 315 320
tcc tga cctggtacac ctggacgccg acctcggcgg cacgaccacc gaggtgatca 1316
Ser
ccgccctggc gggcgtcgtc cacgccgccg gccgcgccag ctccccgcaa ctgctcgccg 1376
acgccgccct cgcccgtgag gcccagaacc ccaccggtgt gcccggccgc gtcgccatcc 1436
cccactgccg ttccgccgcc gtcgaggtcc cgaccctggc cttcgcccga ctgtcgcgcc 1496
ccgtcgactt cggcggtccc gacggcgacg ccgagctcgt cttcctcatc gccgcccccg 1556
agggcggcgg 1566
<210> 160
<211> 321
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 160
Val Ile Leu Thr Leu Thr Pro Asn Pro Ser Ile Asp Ala Thr Met Gln
1 5 10 15
Leu Gly Thr Arg Leu Arg Arg Gly Leu Val His Arg Pro Ser Ser Ile
20 25 30
Ser Gln Val Ala Gly Gly Lys Gly Val Asn Val Thr His Ala Leu Thr
35 40 45
Leu Ala Asp Lys Leu Ser Leu Ala Leu Phe Pro Ala Asp Gly Asn Asp
50 55 60
Pro Phe Val Ala Leu Ala Arg Gln Ser Gly Ile Pro Phe His Ala Ile
65 70 75 80
Pro Val Ser Glu Gly Val Arg Ile Asn Thr Thr Leu Thr Glu Pro Asp
85 90 95
Gly Thr Thr Thr Lys Ile Asn Gly Leu Gly Ala Gln Ile Glu Pro Pro
100 105 110
Thr Arg Arg Ile Ile Glu Glu Thr Val Val Arg Leu Ala Ala Asn Ala
115 120 125
Ser Trp Leu Val Met Ala Gly Ser Leu Pro Pro Gly Val Pro Thr Gly
130 135 140
Trp Tyr Thr Glu Leu Ile Thr Thr Val Arg Ala Thr His Pro Asp Leu
145 150 155 160
Pro Val Ala Val Asp Thr Ser Asp Ala Ala Met Ile Ala Leu Gly Glu
165 170 175
Gly Phe Glu Val Ala Ala Pro Thr Leu Ile Lys Pro Asn Gly Met Glu
180 185 190
Leu Gly Gln Leu Val Asp Arg Asp Gly Glu His Leu Glu Ala Ala Ala
195 200 205
Ala Arg Gly Asp Phe Ser Pro Val Ile Asp Ala Ala Arg Val Val Val
210 215 220
Asp Arg Gly Val Asn Glu Val Leu Val Thr Leu Gly Val Ala Gly Ala
225 230 235 240
Ala Leu Val Thr Ala Gln Gly Val Trp Leu Ala Thr Pro Pro Pro Val
245 250 255
Asp Val Met Ser Thr Val Gly Ala Gly Asp Ser Ser Leu Ala Gly Tyr
260 265 270
Leu Met Ala Arg Ala Glu Gly Gly Ser Pro Ala Asp Cys Leu Ala Arg
275 280 285
Ala Val Ala Tyr Gly Thr Ala Ala Thr Gly Leu Pro Gly Thr Thr Ile
290 295 300
Pro Ser Pro Ala Gln Leu Asn Leu Glu Glu Thr His Val Arg His Ile
305 310 315 320
Ser
<210> 161
<211> 1632
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1332)
<223> ген 6-фосфофруктокиназы
<400> 161
tggtgtgggc gcccaactcg gcgacggcga tgcggaccgt cgacatcgcc tacatgggtg 60
cggcggccgg cgtgtacaac acggggcgcc agaccggtgc ggtcctcggc gcggccgggg 120
tgggggcggc gatgcaggtc ggtgcggcca cggccgggtt cgtgccgggt ctcgctctgt 180
cgctgctgct ccccgtggcc gtcctcggtc tggggctggt ggcggtcgcc ttcttcgaga 240
ccccgccaca cgccgaacca cagggtgccg aacagcgggc cggggactaa agtggtctcc 300
atg cga ctt gcc acc ctc acc tcc ggc ggc gac tgc ccc ggc ctc aac 348
Met Arg Leu Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro Gly Leu Asn
1 5 10 15
gcc gtc atc cgc ggc atc gtc cgt acc gcc agc tcc gag ttc ggt tcc 396
Ala Val Ile Arg Gly Ile Val Arg Thr Ala Ser Ser Glu Phe Gly Ser
20 25 30
acc gtc gtg gga tac cag gac ggc tgg cag ggc ctg ctg gag gac cgc 444
Thr Val Val Gly Tyr Gln Asp Gly Trp Gln Gly Leu Leu Glu Asp Arg
35 40 45
cgc gtc gac ctc tac gac gac gag cac atc gac cgc atc ctc ctg cgc 492
Arg Val Asp Leu Tyr Asp Asp Glu His Ile Asp Arg Ile Leu Leu Arg
50 55 60
ggt ggc acg atc ctg ggc acc ggt cgt ctg cac ccg gac aag ttc aag 540
Gly Gly Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp Lys Phe Lys
65 70 75 80
gcg ggg ctc gac cag atc aag cag aac ctg gat gac gcg ggc atc gac 588
Ala Gly Leu Asp Gln Ile Lys Gln Asn Leu Asp Asp Ala Gly Ile Asp
85 90 95
gcc ctc atc ccg atc ggc ggc gag ggc acc ctc aag ggc gcg aag tgg 636
Ala Leu Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly Ala Lys Trp
100 105 110
ctg tcc gac aac ggc atc ccg gtc gtc ggc gtg ccg aag acc atc gac 684
Leu Ser Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys Thr Ile Asp
115 120 125
aac gac gtc tac ggc acc gac tac acc ttc ggc ttc gac acc gcc gtc 732
Asn Asp Val Tyr Gly Thr Asp Tyr Thr Phe Gly Phe Asp Thr Ala Val
130 135 140
gcc gtg gcc acc gac gcc gtc gac cgt ctg cac acc acc gcc gag tcc 780
Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr Ala Glu Ser
145 150 155 160
cac aac cgc atc ctc atc gtc gag gtc atg ggc cgt cac gtc ggc tgg 828
His Asn Arg Ile Leu Ile Val Glu Val Met Gly Arg His Val Gly Trp
165 170 175
atc gcg ctg cac gcg ggc atg gcc ggc ggc gcg cac tac acc gtg atc 876
Ile Ala Leu His Ala Gly Met Ala Gly Gly Ala His Tyr Thr Val Ile
180 185 190
ccg gag gtg ccc ttc gac atc tcc gag atc tgc aag aag atg gag cgc 924
Pro Glu Val Pro Phe Asp Ile Ser Glu Ile Cys Lys Lys Met Glu Arg
195 200 205
cgc ttc cag atg ggc gag aag tac ggc atc atc gtc gtc gcc gag ggc 972
Arg Phe Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val Ala Glu Gly
210 215 220
gcc gcg ccc aag gag ggc acg atg gag ctc aag tcc ggt ggc gtc gac 1020
Ala Ala Pro Lys Glu Gly Thr Met Glu Leu Lys Ser Gly Gly Val Asp
225 230 235 240
cag ttc ggc cac gag atc ttc acc ggc atc ggc cag cag atc gcc gac 1068
Gln Phe Gly His Glu Ile Phe Thr Gly Ile Gly Gln Gln Ile Ala Asp
245 250 255
gag atc cac gcc cgc ctc ggc cat gac gtc cgc acc tcc gtc ctg ggc 1116
Glu Ile His Ala Arg Leu Gly His Asp Val Arg Thr Ser Val Leu Gly
260 265 270
cac atc cag cgc ggt ggc acc ccg acc gcc ttc gac cgc gtc ctg gcc 1164
His Ile Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg Val Leu Ala
275 280 285
acc cgt tac ggc gtc cgc gcg acc cgc tcc gcc cac cgc ggc gag ttc 1212
Thr Arg Tyr Gly Val Arg Ala Thr Arg Ser Ala His Arg Gly Glu Phe
290 295 300
ggc acc tgc gtg gcg ctc aag ggt gag cgg atc gag aac atc acg ctg 1260
Gly Thr Cys Val Ala Leu Lys Gly Glu Arg Ile Glu Asn Ile Thr Leu
305 310 315 320
gag aag gcc gtg ggc acc ctc aag acc gtg ccg atg gac cgc tgg ctc 1308
Glu Lys Ala Val Gly Thr Leu Lys Thr Val Pro Met Asp Arg Trp Leu
325 330 335
acc gcc cag gcc atg ttc ggc tag gtgcgcgacc tccatctgga cacctgccgg 1362
Thr Ala Gln Ala Met Phe Gly
340
ctgctcgtcg tcggcatcaa cccgggtctg gtgtcggagc gcgtcgacgc gcccttcgcc 1422
cgcccgggca accgtttctg gccggcgctc cacgccgccg gcatcacgga gacgcttgtc 1482
gacgcccgcg acggcctcac cgccgcccac cacgcggagc tgctgcggcg gggcctcggc 1542
ttcacgaacg tcgtgccgcg ggccacggcg gtggcctccg agctgacccg cgaggagtac 1602
ctggcgggtg gggcggtgct ggcggagaag 1632
<210> 162
<211> 343
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 162
Met Arg Leu Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro Gly Leu Asn
1 5 10 15
Ala Val Ile Arg Gly Ile Val Arg Thr Ala Ser Ser Glu Phe Gly Ser
20 25 30
Thr Val Val Gly Tyr Gln Asp Gly Trp Gln Gly Leu Leu Glu Asp Arg
35 40 45
Arg Val Asp Leu Tyr Asp Asp Glu His Ile Asp Arg Ile Leu Leu Arg
50 55 60
Gly Gly Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp Lys Phe Lys
65 70 75 80
Ala Gly Leu Asp Gln Ile Lys Gln Asn Leu Asp Asp Ala Gly Ile Asp
85 90 95
Ala Leu Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly Ala Lys Trp
100 105 110
Leu Ser Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys Thr Ile Asp
115 120 125
Asn Asp Val Tyr Gly Thr Asp Tyr Thr Phe Gly Phe Asp Thr Ala Val
130 135 140
Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr Ala Glu Ser
145 150 155 160
His Asn Arg Ile Leu Ile Val Glu Val Met Gly Arg His Val Gly Trp
165 170 175
Ile Ala Leu His Ala Gly Met Ala Gly Gly Ala His Tyr Thr Val Ile
180 185 190
Pro Glu Val Pro Phe Asp Ile Ser Glu Ile Cys Lys Lys Met Glu Arg
195 200 205
Arg Phe Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val Ala Glu Gly
210 215 220
Ala Ala Pro Lys Glu Gly Thr Met Glu Leu Lys Ser Gly Gly Val Asp
225 230 235 240
Gln Phe Gly His Glu Ile Phe Thr Gly Ile Gly Gln Gln Ile Ala Asp
245 250 255
Glu Ile His Ala Arg Leu Gly His Asp Val Arg Thr Ser Val Leu Gly
260 265 270
His Ile Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg Val Leu Ala
275 280 285
Thr Arg Tyr Gly Val Arg Ala Thr Arg Ser Ala His Arg Gly Glu Phe
290 295 300
Gly Thr Cys Val Ala Leu Lys Gly Glu Arg Ile Glu Asn Ile Thr Leu
305 310 315 320
Glu Lys Ala Val Gly Thr Leu Lys Thr Val Pro Met Asp Arg Trp Leu
325 330 335
Thr Ala Gln Ala Met Phe Gly
340
<210> 163
<211> 1932
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1632)
<223> ген lysA
<400> 163
cggacctctc cctgctcacc cacgagcggg agggcgacct catccgcacc ctcggcgagt 60
tcccgaacgt ggtcaaggcc gcggcggaac tgcgtgagcc gcaccgtatc gcccgttatg 120
ccgaggaact ggccggcgtg ttccaccgct tctacgacaa ctgccagatc ctgccgaagg 180
cggacgaaga ggccgccccg atccacaccg cccgcctggc gctggctgcg gccacccgcc 240
agaccctggc caacgcgctc ggcctggtcg gtgtcaccgc cccggagagg atgtaagcgc 300
atg acc tcg gat ttc aac gat ctg ccg gcc cac gtc tgg ccg cgc aac 348
Met Thr Ser Asp Phe Asn Asp Leu Pro Ala His Val Trp Pro Arg Asn
1 5 10 15
gcc gcg cgc cag gag gac ggc gtg gtc acc gtc gcg ggt gtc ccg ctg 396
Ala Ala Arg Gln Glu Asp Gly Val Val Thr Val Ala Gly Val Pro Leu
20 25 30
ccg gag atc gcc gag gag ttc ggt acc ccg gtc ttc gtc gtc gac gag 444
Pro Glu Ile Ala Glu Glu Phe Gly Thr Pro Val Phe Val Val Asp Glu
35 40 45
gac gac ttc cgt tcc cgc tgc cag gac atg gcc cgc gcc ttc ggc ggc 492
Asp Asp Phe Arg Ser Arg Cys Gln Asp Met Ala Arg Ala Phe Gly Gly
50 55 60
ccg gag cac gtc cac tac gcc tcc aag gcg ttc ctc acc cgc acc gtc 540
Pro Glu His Val His Tyr Ala Ser Lys Ala Phe Leu Thr Arg Thr Val
65 70 75 80
gcc cgc tgg gtc gac gag gag ggg ctg tcg ctc gac gtc gcc tcg ctc 588
Ala Arg Trp Val Asp Glu Glu Gly Leu Ser Leu Asp Val Ala Ser Leu
85 90 95
aac gag ctg cgg atc gcg ctg gcc gcc gag ttc ccc gcc gcc cgc atc 636
Asn Glu Leu Arg Ile Ala Leu Ala Ala Glu Phe Pro Ala Ala Arg Ile
100 105 110
acc gcg cac ggc aac aac aag ggc ccg tcc ttc ctg cgg gcg tgc gtc 684
Thr Ala His Gly Asn Asn Lys Gly Pro Ser Phe Leu Arg Ala Cys Val
115 120 125
acc gag ggc gtc ggc cac gtc gtc ctc gac tcc gag cag gag ctc gag 732
Thr Glu Gly Val Gly His Val Val Leu Asp Ser Glu Gln Glu Leu Glu
130 135 140
ctt ctc gac gcc atc gcc gcc ggc gcc ggc gtc atc cag aag gtg atg 780
Leu Leu Asp Ala Ile Ala Ala Gly Ala Gly Val Ile Gln Lys Val Met
145 150 155 160
atc cgc gtc aag ccg ggc atc gag gcg cac acc cac gag gcc atc gcc 828
Ile Arg Val Lys Pro Gly Ile Glu Ala His Thr His Glu Ala Ile Ala
165 170 175
acg tcc cac gag gac cag aag ttc ggc ttc tcc ctg gcc tcc ggc tcg 876
Thr Ser His Glu Asp Gln Lys Phe Gly Phe Ser Leu Ala Ser Gly Ser
180 185 190
gcg ttc cgc gcc gcg gag gcc gcc gtc cgc gcc gag aac ctc gag ctc 924
Ala Phe Arg Ala Ala Glu Ala Ala Val Arg Ala Glu Asn Leu Glu Leu
195 200 205
gtg ggc ctg cac tgc cac gtc ggc tcc cag gtc ttc gac gcc cgg ggc 972
Val Gly Leu His Cys His Val Gly Ser Gln Val Phe Asp Ala Arg Gly
210 215 220
ttc acg atg gcc gcc gac cgc gtg ctg ggc ctc tac tcg cag atc cac 1020
Phe Thr Met Ala Ala Asp Arg Val Leu Gly Leu Tyr Ser Gln Ile His
225 230 235 240
cgc gaa ctc ggc gtg cag ctg ccc gag ctc gat ctc ggc ggc ggc tac 1068
Arg Glu Leu Gly Val Gln Leu Pro Glu Leu Asp Leu Gly Gly Gly Tyr
245 250 255
ggc atc gcc tac acc gag gac gag cag ccc ctc aac gtc gac gag gtc 1116
Gly Ile Ala Tyr Thr Glu Asp Glu Gln Pro Leu Asn Val Asp Glu Val
260 265 270
gcc cac gac ctg ctc acc gcc gtg gcc aag acc gcc gcg gag ctg ggc 1164
Ala His Asp Leu Leu Thr Ala Val Ala Lys Thr Ala Ala Glu Leu Gly
275 280 285
atc gac gcc ccc gcc gtc ctc gtc gag ccg ggc cgc gcc atc gcc ggc 1212
Ile Asp Ala Pro Ala Val Leu Val Glu Pro Gly Arg Ala Ile Ala Gly
290 295 300
ccc ggc acg gtg acg gtc tac gag gtc ggc acc gtc aag gac gtc acc 1260
Pro Gly Thr Val Thr Val Tyr Glu Val Gly Thr Val Lys Asp Val Thr
305 310 315 320
gtc tcc gag gac gag aag cga cgc tac ctc tcc gtc gac ggc ggc atg 1308
Val Ser Glu Asp Glu Lys Arg Arg Tyr Leu Ser Val Asp Gly Gly Met
325 330 335
tcc gac aac atc cgc ccg gcc ctc tac gac gcg ctt tac gac gcc cgc 1356
Ser Asp Asn Ile Arg Pro Ala Leu Tyr Asp Ala Leu Tyr Asp Ala Arg
340 345 350
gtg gtc aac cgt ttc acc gag ggc gag gag atc tcc tcc cgc ctc gtc 1404
Val Val Asn Arg Phe Thr Glu Gly Glu Glu Ile Ser Ser Arg Leu Val
355 360 365
ggt tcc cac tgc gag tcc ggc gac att ctc gtc cgc gac gcg cgc tac 1452
Gly Ser His Cys Glu Ser Gly Asp Ile Leu Val Arg Asp Ala Arg Tyr
370 375 380
ccc gac gac atc gtg gtc ggg gac ctc atg gcc gtc gcc gcg acc ggc 1500
Pro Asp Asp Ile Val Val Gly Asp Leu Met Ala Val Ala Ala Thr Gly
385 390 395 400
gcc tac gcc tac gcc atg tcc tcg cgc tac aac gcc ttc ggc cgt ccg 1548
Ala Tyr Ala Tyr Ala Met Ser Ser Arg Tyr Asn Ala Phe Gly Arg Pro
405 410 415
ccg gtc gtc gcg gtg cgt gcc ggc aag acg aag ctc atg ctg cgc cgc 1596
Pro Val Val Ala Val Arg Ala Gly Lys Thr Lys Leu Met Leu Arg Arg
420 425 430
gag acc gtc gag gac att ctc gcg ctg gag acg tag aaccgacgcc 1642
Glu Thr Val Glu Asp Ile Leu Ala Leu Glu Thr
435 440
cgggcagggc gtcagtggaa gaggcccttg aggctgccgg cgtcctccag cagcgccacg 1702
acgagcgagg tgtggatgga accgagcggc acgcccctgg cgggcgtcgg cgcggtcgac 1762
gggtccgggg tggaggaacg ggtcggcgtc gtggacgact cttcgcggtc gacggtgccg 1822
gaggctcccg tggcgcacat gcgcagctcc gcccggtagg cgtccgcctc cgcctgggct 1882
cccggggtgc gctcccaggc ccgcagccag gcctcgtcgg cggccacgga 1932
<210> 164
<211> 443
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 164
Met Thr Ser Asp Phe Asn Asp Leu Pro Ala His Val Trp Pro Arg Asn
1 5 10 15
Ala Ala Arg Gln Glu Asp Gly Val Val Thr Val Ala Gly Val Pro Leu
20 25 30
Pro Glu Ile Ala Glu Glu Phe Gly Thr Pro Val Phe Val Val Asp Glu
35 40 45
Asp Asp Phe Arg Ser Arg Cys Gln Asp Met Ala Arg Ala Phe Gly Gly
50 55 60
Pro Glu His Val His Tyr Ala Ser Lys Ala Phe Leu Thr Arg Thr Val
65 70 75 80
Ala Arg Trp Val Asp Glu Glu Gly Leu Ser Leu Asp Val Ala Ser Leu
85 90 95
Asn Glu Leu Arg Ile Ala Leu Ala Ala Glu Phe Pro Ala Ala Arg Ile
100 105 110
Thr Ala His Gly Asn Asn Lys Gly Pro Ser Phe Leu Arg Ala Cys Val
115 120 125
Thr Glu Gly Val Gly His Val Val Leu Asp Ser Glu Gln Glu Leu Glu
130 135 140
Leu Leu Asp Ala Ile Ala Ala Gly Ala Gly Val Ile Gln Lys Val Met
145 150 155 160
Ile Arg Val Lys Pro Gly Ile Glu Ala His Thr His Glu Ala Ile Ala
165 170 175
Thr Ser His Glu Asp Gln Lys Phe Gly Phe Ser Leu Ala Ser Gly Ser
180 185 190
Ala Phe Arg Ala Ala Glu Ala Ala Val Arg Ala Glu Asn Leu Glu Leu
195 200 205
Val Gly Leu His Cys His Val Gly Ser Gln Val Phe Asp Ala Arg Gly
210 215 220
Phe Thr Met Ala Ala Asp Arg Val Leu Gly Leu Tyr Ser Gln Ile His
225 230 235 240
Arg Glu Leu Gly Val Gln Leu Pro Glu Leu Asp Leu Gly Gly Gly Tyr
245 250 255
Gly Ile Ala Tyr Thr Glu Asp Glu Gln Pro Leu Asn Val Asp Glu Val
260 265 270
Ala His Asp Leu Leu Thr Ala Val Ala Lys Thr Ala Ala Glu Leu Gly
275 280 285
Ile Asp Ala Pro Ala Val Leu Val Glu Pro Gly Arg Ala Ile Ala Gly
290 295 300
Pro Gly Thr Val Thr Val Tyr Glu Val Gly Thr Val Lys Asp Val Thr
305 310 315 320
Val Ser Glu Asp Glu Lys Arg Arg Tyr Leu Ser Val Asp Gly Gly Met
325 330 335
Ser Asp Asn Ile Arg Pro Ala Leu Tyr Asp Ala Leu Tyr Asp Ala Arg
340 345 350
Val Val Asn Arg Phe Thr Glu Gly Glu Glu Ile Ser Ser Arg Leu Val
355 360 365
Gly Ser His Cys Glu Ser Gly Asp Ile Leu Val Arg Asp Ala Arg Tyr
370 375 380
Pro Asp Asp Ile Val Val Gly Asp Leu Met Ala Val Ala Ala Thr Gly
385 390 395 400
Ala Tyr Ala Tyr Ala Met Ser Ser Arg Tyr Asn Ala Phe Gly Arg Pro
405 410 415
Pro Val Val Ala Val Arg Ala Gly Lys Thr Lys Leu Met Leu Arg Arg
420 425 430
Glu Thr Val Glu Asp Ile Leu Ala Leu Glu Thr
435 440
<210> 165
<211> 1770
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1470)
<223> ген aat
<400> 165
cgcctacgcg ctcaccgtcg tcctggccgc cgagaactac ccggccgccc cggtcaccgg 60
tggggtcatc accggtgacg tcgtgaatga cccgtccaag gtcctccacg ccggtaccgc 120
gcgcaacgag gccggcgagc tggtctccgc cggtggccgc gtgctcaaca tcgtgggcgt 180
cggcaagacc ctcgaggagg cgcgtgacga cgcctacgcc accatcgagc agatccagct 240
cgagggttcc ttctaccgcg gcgacatcgc actgccggcc gtggagggac ggatctccct 300
gtg acc ggt cgg cag ctc gtc agc gac cgc ggc cag gtc cag ccc ttc 348
Val Thr Gly Arg Gln Leu Val Ser Asp Arg Gly Gln Val Gln Pro Phe
1 5 10 15
cgc gtc atg cag atg ctc gac atc gtg cac cga cgc gcc cgc gag ggg 396
Arg Val Met Gln Met Leu Asp Ile Val His Arg Arg Ala Arg Glu Gly
20 25 30
aag cac acc atc atg ctg tgc gcc ggg cag ccc tcc acg ggt gcc ccg 444
Lys His Thr Ile Met Leu Cys Ala Gly Gln Pro Ser Thr Gly Ala Pro
35 40 45
gcc ccg gtc ctg acc gcg gcg gag gag tcc ctc cac gcc tcg ccc ctc 492
Ala Pro Val Leu Thr Ala Ala Glu Glu Ser Leu His Ala Ser Pro Leu
50 55 60
ggg tac acc gag gtg gtc ggt gac cgc gac ctc cgg gag atc gtc gcg 540
Gly Tyr Thr Glu Val Val Gly Asp Arg Asp Leu Arg Glu Ile Val Ala
65 70 75 80
gcc tgg cac tcc gac acc tac ggc gtg cac acc agc gcg gac aac gtg 588
Ala Trp His Ser Asp Thr Tyr Gly Val His Thr Ser Ala Asp Asn Val
85 90 95
gtg atc acc acc ggc tcc tcc ggt ggc ttc gtc gcg tcc ttc ctg gcc 636
Val Ile Thr Thr Gly Ser Ser Gly Gly Phe Val Ala Ser Phe Leu Ala
100 105 110
gtg ctc gac cac ggc gac gcc gtc gcc ctg tcc tgc ccc ggt tac ccg 684
Val Leu Asp His Gly Asp Ala Val Ala Leu Ser Cys Pro Gly Tyr Pro
115 120 125
gcg tac cgc aac atc ctg gag tcc ctc ggg gcg cgg gtg gtc aac ctc 732
Ala Tyr Arg Asn Ile Leu Glu Ser Leu Gly Ala Arg Val Val Asn Leu
130 135 140
gtg tgc gac gag tcg acc cgc ttc cag ccg acg gcc gcg atg ctc gag 780
Val Cys Asp Glu Ser Thr Arg Phe Gln Pro Thr Ala Ala Met Leu Glu
145 150 155 160
gca ctg ccg gag gct gac agg ccg cgg gcg gtg atc gtc acc tcc ccg 828
Ala Leu Pro Glu Ala Asp Arg Pro Arg Ala Val Ile Val Thr Ser Pro
165 170 175
ggc aac ccc tcc ggc acg acc atc gac ccc gcg gag ctc gag cgc atc 876
Gly Asn Pro Ser Gly Thr Thr Ile Asp Pro Ala Glu Leu Glu Arg Ile
180 185 190
gcc cgc tgg tgc gag gac aac gac gcc gtg ctc atc tcc gac gag gac 924
Ala Arg Trp Cys Glu Asp Asn Asp Ala Val Leu Ile Ser Asp Glu Asp
195 200 205
tac cac ggc atg tcc ttc ggc cgc ccc ctg gcc acc gca cgc cag ttc 972
Tyr His Gly Met Ser Phe Gly Arg Pro Leu Ala Thr Ala Arg Gln Phe
210 215 220
tcc gac cgg gcg atc gtc gtg ggc acg ctg tcg aag tac tac tcc atg 1020
Ser Asp Arg Ala Ile Val Val Gly Thr Leu Ser Lys Tyr Tyr Ser Met
225 230 235 240
acc ggc tgg cgc gtc ggc tgg ctc atc ctc ccc gac gag ctg gtc acc 1068
Thr Gly Trp Arg Val Gly Trp Leu Ile Leu Pro Asp Glu Leu Val Thr
245 250 255
ccc gtg gag aac ctg cag gcc tcg ctg gcg ctc tgc ccg ccg gcg atc 1116
Pro Val Glu Asn Leu Gln Ala Ser Leu Ala Leu Cys Pro Pro Ala Ile
260 265 270
tcg cag gtc gcc ggg cgt gcc gcc ttc acc ccg gag gcg cgc gcc gaa 1164
Ser Gln Val Ala Gly Arg Ala Ala Phe Thr Pro Glu Ala Arg Ala Glu
275 280 285
ctc gac tcc cac gtc gag cgc tac cgc gcc gcc cgc gcg gtg ctg ctc 1212
Leu Asp Ser His Val Glu Arg Tyr Arg Ala Ala Arg Ala Val Leu Leu
290 295 300
gag gag ctg ccg gcg gcc ggt ttc acc acc atg gcg gaa ccc gac ggc 1260
Glu Glu Leu Pro Ala Ala Gly Phe Thr Thr Met Ala Glu Pro Asp Gly
305 310 315 320
ggg ctc tac ctg tgg ctc gac gtc tcc cac gtc acc gac gac tcc gag 1308
Gly Leu Tyr Leu Trp Leu Asp Val Ser His Val Thr Asp Asp Ser Glu
325 330 335
gcc tgg tgc cac gag ctc gtc gag gag atc ggc gtg gcc ttc gcg ccg 1356
Ala Trp Cys His Glu Leu Val Glu Glu Ile Gly Val Ala Phe Ala Pro
340 345 350
ggc gtc gac ttc gac ccg atc aac ggt cac aag tgg gtc cgc ctc acg 1404
Gly Val Asp Phe Asp Pro Ile Asn Gly His Lys Trp Val Arg Leu Thr
355 360 365
ctg tgc gcc ccg gag gag gag acc cgc gag gcg atc cgg cgc ctc ggc 1452
Leu Cys Ala Pro Glu Glu Glu Thr Arg Glu Ala Ile Arg Arg Leu Gly
370 375 380
gag tac gtg cgt cgc tga ccgcgcggcg acctgcggcg cgggggaaac 1500
Glu Tyr Val Arg Arg
385
ggcccggatc cggggactcc cgggccaccc ccgccgctgg tcatgcgata atcgggggcg 1560
tggctgagaa gaagaagatc gcgaacgtcc tgtccaaccg ctacgcctcc gcggagctga 1620
gcaacctgtg gagcccggag tacaagatca tcctggagcg tcagctctgg atcgccgtga 1680
tgaaggcgca gaaggatctg ggtgtcgaca tcccggctga ggccatcgac gcctacgagg 1740
ccgtcatcga ccgggtcgac ctcgagtcga 1770
<210> 166
<211> 389
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 166
Val Thr Gly Arg Gln Leu Val Ser Asp Arg Gly Gln Val Gln Pro Phe
1 5 10 15
Arg Val Met Gln Met Leu Asp Ile Val His Arg Arg Ala Arg Glu Gly
20 25 30
Lys His Thr Ile Met Leu Cys Ala Gly Gln Pro Ser Thr Gly Ala Pro
35 40 45
Ala Pro Val Leu Thr Ala Ala Glu Glu Ser Leu His Ala Ser Pro Leu
50 55 60
Gly Tyr Thr Glu Val Val Gly Asp Arg Asp Leu Arg Glu Ile Val Ala
65 70 75 80
Ala Trp His Ser Asp Thr Tyr Gly Val His Thr Ser Ala Asp Asn Val
85 90 95
Val Ile Thr Thr Gly Ser Ser Gly Gly Phe Val Ala Ser Phe Leu Ala
100 105 110
Val Leu Asp His Gly Asp Ala Val Ala Leu Ser Cys Pro Gly Tyr Pro
115 120 125
Ala Tyr Arg Asn Ile Leu Glu Ser Leu Gly Ala Arg Val Val Asn Leu
130 135 140
Val Cys Asp Glu Ser Thr Arg Phe Gln Pro Thr Ala Ala Met Leu Glu
145 150 155 160
Ala Leu Pro Glu Ala Asp Arg Pro Arg Ala Val Ile Val Thr Ser Pro
165 170 175
Gly Asn Pro Ser Gly Thr Thr Ile Asp Pro Ala Glu Leu Glu Arg Ile
180 185 190
Ala Arg Trp Cys Glu Asp Asn Asp Ala Val Leu Ile Ser Asp Glu Asp
195 200 205
Tyr His Gly Met Ser Phe Gly Arg Pro Leu Ala Thr Ala Arg Gln Phe
210 215 220
Ser Asp Arg Ala Ile Val Val Gly Thr Leu Ser Lys Tyr Tyr Ser Met
225 230 235 240
Thr Gly Trp Arg Val Gly Trp Leu Ile Leu Pro Asp Glu Leu Val Thr
245 250 255
Pro Val Glu Asn Leu Gln Ala Ser Leu Ala Leu Cys Pro Pro Ala Ile
260 265 270
Ser Gln Val Ala Gly Arg Ala Ala Phe Thr Pro Glu Ala Arg Ala Glu
275 280 285
Leu Asp Ser His Val Glu Arg Tyr Arg Ala Ala Arg Ala Val Leu Leu
290 295 300
Glu Glu Leu Pro Ala Ala Gly Phe Thr Thr Met Ala Glu Pro Asp Gly
305 310 315 320
Gly Leu Tyr Leu Trp Leu Asp Val Ser His Val Thr Asp Asp Ser Glu
325 330 335
Ala Trp Cys His Glu Leu Val Glu Glu Ile Gly Val Ala Phe Ala Pro
340 345 350
Gly Val Asp Phe Asp Pro Ile Asn Gly His Lys Trp Val Arg Leu Thr
355 360 365
Leu Cys Ala Pro Glu Glu Glu Thr Arg Glu Ala Ile Arg Arg Leu Gly
370 375 380
Glu Tyr Val Arg Arg
385
<210> 167
<211> 1281
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(981)
<223> ген lysE
<400> 167
gaaggcgatc acctggatgc cgtagcccac cgccgccggg tcgaggcgcg tgtggaagcc 60
ggtgatcacg cccccgttct ccagcgcccg cagccgccgc aggcaggtgc ttgacgacgc 120
ccccgtatga tgcgccagtt ctgcggcggt gatgcgtgca ttgttctgga tcagtgacag 180
aatcatgcgg tcaatgtcgt ccatacgccg gattctgtca cacgtgcagt cccggcgtcc 240
cccggtgaat gaaaagttca ggagtatcgg ccgcggcgcc gggcgggcac gataccgggc 300
atg gac atc acg gac ctc ctg agc ttc tgg gcg gtc agc ctc ctg ctg 348
Met Asp Ile Thr Asp Leu Leu Ser Phe Trp Ala Val Ser Leu Leu Leu
1 5 10 15
atc gtc acc ccg ggc ccc gac tgg gcg ttc gtg ctc ggg cac gct ttc 396
Ile Val Thr Pro Gly Pro Asp Trp Ala Phe Val Leu Gly His Ala Phe
20 25 30
cga cgc cgc ccc ctc gcc ccg ccc ctt ctc ggc atc ggc ctg ggc tac 444
Arg Arg Arg Pro Leu Ala Pro Pro Leu Leu Gly Ile Gly Leu Gly Tyr
35 40 45
ctc ggc ctg acc acc gtg gtg gcc ggc ggc ctc ggg gcg ctc gtg gcc 492
Leu Gly Leu Thr Thr Val Val Ala Gly Gly Leu Gly Ala Leu Val Ala
50 55 60
cgt cac ccc gcg ctg ctc acg ctc gtc acc gtc gcc ggt gtc ctg gtg 540
Arg His Pro Ala Leu Leu Thr Leu Val Thr Val Ala Gly Val Leu Val
65 70 75 80
ctc ctg cgt ctc ggc tgg cag atg atg cgc gcg gcg ctg gcc ggg ccc 588
Leu Leu Arg Leu Gly Trp Gln Met Met Arg Ala Ala Leu Ala Gly Pro
85 90 95
gtc acg gtg gag gtg gac acc gcg gac ccg gct gtc ccg gag ggg ccg 636
Val Thr Val Glu Val Asp Thr Ala Asp Pro Ala Val Pro Glu Gly Pro
100 105 110
gcc ggc ggg gga gtg gcg acg ctg acg cgc ccg cag gtg ggc gtc gag 684
Ala Gly Gly Gly Val Ala Thr Leu Thr Arg Pro Gln Val Gly Val Glu
115 120 125
aag cac gcg gtg gtc acc ggg gcc gcg gtg agt ggc ctc aac ccc aag 732
Lys His Ala Val Val Thr Gly Ala Ala Val Ser Gly Leu Asn Pro Lys
130 135 140
ggg ctc atg ctg ttc atc gcg ctg ctg ccc cag ttc gtg ggc gcg gag 780
Gly Leu Met Leu Phe Ile Ala Leu Leu Pro Gln Phe Val Gly Ala Glu
145 150 155 160
gcg acg tgg ccg gtg tgg acg cag atg ttc gtc ctc gga ctg gtg ttc 828
Ala Thr Trp Pro Val Trp Thr Gln Met Phe Val Leu Gly Leu Val Phe
165 170 175
atc gcc tcg gcg acc gtg ttc tac gcg ctg ctc ggc gtt ttc gcc ggg 876
Ile Ala Ser Ala Thr Val Phe Tyr Ala Leu Leu Gly Val Phe Ala Gly
180 185 190
cgg gtg ctg gcg ggc tcg gag cgg gcc tcc cgg atc ctc acc ggc gtg 924
Arg Val Leu Ala Gly Ser Glu Arg Ala Ser Arg Ile Leu Thr Gly Val
195 200 205
gcc ggc ggg gcg atg atg tgt gtc gcg gca ggg atg ctc gcg cag cac 972
Ala Gly Gly Ala Met Met Cys Val Ala Ala Gly Met Leu Ala Gln His
210 215 220
ctc ctc tga tgggggtagt tgagtagggg cattcatcgg gtgcgagttc 1021
Leu Leu
225
cgcgcctccc gatgaatgcc cctgctcgga taccctccta ctccacgtgc gccggcgtcg 1081
acggcgacac cagcggcatc ggcgcgtact cggcacgcag ctcgcgcagc ttccgggcgt 1141
gctcggagag gcggacgtcc tcggtggtac gggggacgaa ctggcgggcg ggcagggcgt 1201
tgccgtcggc gtccgtcgcc aggtaggtga cggtggcgtg gatggcggtc tgcaggttct 1261
cgcggccccc gcgcgggttg 1281
<210> 168
<211> 226
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 168
Met Asp Ile Thr Asp Leu Leu Ser Phe Trp Ala Val Ser Leu Leu Leu
1 5 10 15
Ile Val Thr Pro Gly Pro Asp Trp Ala Phe Val Leu Gly His Ala Phe
20 25 30
Arg Arg Arg Pro Leu Ala Pro Pro Leu Leu Gly Ile Gly Leu Gly Tyr
35 40 45
Leu Gly Leu Thr Thr Val Val Ala Gly Gly Leu Gly Ala Leu Val Ala
50 55 60
Arg His Pro Ala Leu Leu Thr Leu Val Thr Val Ala Gly Val Leu Val
65 70 75 80
Leu Leu Arg Leu Gly Trp Gln Met Met Arg Ala Ala Leu Ala Gly Pro
85 90 95
Val Thr Val Glu Val Asp Thr Ala Asp Pro Ala Val Pro Glu Gly Pro
100 105 110
Ala Gly Gly Gly Val Ala Thr Leu Thr Arg Pro Gln Val Gly Val Glu
115 120 125
Lys His Ala Val Val Thr Gly Ala Ala Val Ser Gly Leu Asn Pro Lys
130 135 140
Gly Leu Met Leu Phe Ile Ala Leu Leu Pro Gln Phe Val Gly Ala Glu
145 150 155 160
Ala Thr Trp Pro Val Trp Thr Gln Met Phe Val Leu Gly Leu Val Phe
165 170 175
Ile Ala Ser Ala Thr Val Phe Tyr Ala Leu Leu Gly Val Phe Ala Gly
180 185 190
Arg Val Leu Ala Gly Ser Glu Arg Ala Ser Arg Ile Leu Thr Gly Val
195 200 205
Ala Gly Gly Ala Met Met Cys Val Ala Ala Gly Met Leu Ala Gln His
210 215 220
Leu Leu
225
<210> 169
<211> 1347
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1047)
<223> ген dapB
<400> 169
ccggtgccga aggagtcgag ccaggtgcgc agggagttgc ggttgactcc gagatcggag 60
gcgatcgcgt tgatcgttgc tccgggagtc gactcgtaca acgccactgc gtcacgcttg 120
aactgctcgg tgtaggtctt gcgtggcatg gtggaaaggt acctcacttc cccagcgaaa 180
tgctggtttc agcgtgtcca ccatcaaggg gtcaggtccg ctggtgatgt tgatgcgcat 240
gcaacgattc tatgcgccta gactggggca ctgaatcttt caggaactca aggaggaacc 300
atg gcg atc aag gtg gga gtg ctc ggc gcg aag ggc cgt gtc ggc tcg 348
Met Ala Ile Lys Val Gly Val Leu Gly Ala Lys Gly Arg Val Gly Ser
1 5 10 15
gcg gtg tgc gag ggc gtc aac ggg gcg gag ggc ctc gag ctc gtc gcg 396
Ala Val Cys Glu Gly Val Asn Gly Ala Glu Gly Leu Glu Leu Val Ala
20 25 30
cag gtc gat cag gac gat gac ctg cag ctg ctt ctc gac gcc ggg gcg 444
Gln Val Asp Gln Asp Asp Asp Leu Gln Leu Leu Leu Asp Ala Gly Ala
35 40 45
gag gtc gtc gtc gac ttc acc act ccg ggt tcc gtg atg ggc aac ctg 492
Glu Val Val Val Asp Phe Thr Thr Pro Gly Ser Val Met Gly Asn Leu
50 55 60
gag ttc tgc atc gcc aac ggc atc cac gcg gtg gtc ggc acc acc ggc 540
Glu Phe Cys Ile Ala Asn Gly Ile His Ala Val Val Gly Thr Thr Gly
65 70 75 80
ttc gac gag gag cgc ctc gcg cag gtc cgt gag tgg tgt gcc gcc gag 588
Phe Asp Glu Glu Arg Leu Ala Gln Val Arg Glu Trp Cys Ala Ala Glu
85 90 95
ggg gcg ggc aac gtc ctc atc gcc ccg aac ttc gcg atc tct gca gtg 636
Gly Ala Gly Asn Val Leu Ile Ala Pro Asn Phe Ala Ile Ser Ala Val
100 105 110
ctg gcc atg gag ttc gcg cgg cag gcc gcc cgc ttc ttc gac tcc gcc 684
Leu Ala Met Glu Phe Ala Arg Gln Ala Ala Arg Phe Phe Asp Ser Ala
115 120 125
gag gtc gtc gag tac cac cac ccc tac aag ctg gac gcc ccc tcc ggc 732
Glu Val Val Glu Tyr His His Pro Tyr Lys Leu Asp Ala Pro Ser Gly
130 135 140
acc gcg atc cac acc gcg cag ggc atc gcc gcc gcc cgc cgg gag gcc 780
Thr Ala Ile His Thr Ala Gln Gly Ile Ala Ala Ala Arg Arg Glu Ala
145 150 155 160
ggc ctc ggc ccc atc ccg gat gcc acc gag cag acc ctc gag ggt gcc 828
Gly Leu Gly Pro Ile Pro Asp Ala Thr Glu Gln Thr Leu Glu Gly Ala
165 170 175
cgc ggc gcc gac gtc gac ggc gtg cgt gtc cac ggc gtg cgc atg acc 876
Arg Gly Ala Asp Val Asp Gly Val Arg Val His Gly Val Arg Met Thr
180 185 190
ggc atg gtc gcc cac gag gac atc atc ttc ggt gcc cag ggc cag tcc 924
Gly Met Val Ala His Glu Asp Ile Ile Phe Gly Ala Gln Gly Gln Ser
195 200 205
ctg acc atc cgc cag gac tcc tac gac cgc acc tcc ttc gtg ccg ggc 972
Leu Thr Ile Arg Gln Asp Ser Tyr Asp Arg Thr Ser Phe Val Pro Gly
210 215 220
gtg ctc ctc ggt gtc cgt gag atc gcg aac cac gac ggc ctg acc gtg 1020
Val Leu Leu Gly Val Arg Glu Ile Ala Asn His Asp Gly Leu Thr Val
225 230 235 240
ggg ctc gac agc tac ctg gga ctc tag atggccaagc ccgtcgacct 1067
Gly Leu Asp Ser Tyr Leu Gly Leu
245
ggacgtccag ctcatcgcgg ccacgcagtt caccccgccc gccggggtcg actgggaggc 1127
cgacgacacc gccaccggcg ccgaggcgct cgtcgagttc gcgggccgcg cctgctacga 1187
gaccttcgac aaacccaacc cgcgcaccgc ggccaacgac gcctacctcc gccacatcat 1247
ggaggtcgga cacaccgcac tgctcgagca cgccaccgcg acgctctaca tccgcggcat 1307
ctcccgctcc gccacccacg agctggtccg ccaccgcaac 1347
<210> 170
<211> 248
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 170
Met Ala Ile Lys Val Gly Val Leu Gly Ala Lys Gly Arg Val Gly Ser
1 5 10 15
Ala Val Cys Glu Gly Val Asn Gly Ala Glu Gly Leu Glu Leu Val Ala
20 25 30
Gln Val Asp Gln Asp Asp Asp Leu Gln Leu Leu Leu Asp Ala Gly Ala
35 40 45
Glu Val Val Val Asp Phe Thr Thr Pro Gly Ser Val Met Gly Asn Leu
50 55 60
Glu Phe Cys Ile Ala Asn Gly Ile His Ala Val Val Gly Thr Thr Gly
65 70 75 80
Phe Asp Glu Glu Arg Leu Ala Gln Val Arg Glu Trp Cys Ala Ala Glu
85 90 95
Gly Ala Gly Asn Val Leu Ile Ala Pro Asn Phe Ala Ile Ser Ala Val
100 105 110
Leu Ala Met Glu Phe Ala Arg Gln Ala Ala Arg Phe Phe Asp Ser Ala
115 120 125
Glu Val Val Glu Tyr His His Pro Tyr Lys Leu Asp Ala Pro Ser Gly
130 135 140
Thr Ala Ile His Thr Ala Gln Gly Ile Ala Ala Ala Arg Arg Glu Ala
145 150 155 160
Gly Leu Gly Pro Ile Pro Asp Ala Thr Glu Gln Thr Leu Glu Gly Ala
165 170 175
Arg Gly Ala Asp Val Asp Gly Val Arg Val His Gly Val Arg Met Thr
180 185 190
Gly Met Val Ala His Glu Asp Ile Ile Phe Gly Ala Gln Gly Gln Ser
195 200 205
Leu Thr Ile Arg Gln Asp Ser Tyr Asp Arg Thr Ser Phe Val Pro Gly
210 215 220
Val Leu Leu Gly Val Arg Glu Ile Ala Asn His Asp Gly Leu Thr Val
225 230 235 240
Gly Leu Asp Ser Tyr Leu Gly Leu
245
<210> 171
<211> 2145
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1845)
<223> ген глюкозо-6-фосфат-дегидрогеназы
<400> 171
cgaccctcta cgtcaccgag ctggccggcc cggagaccgt caacaccatg ccggagccga 60
ccatcgacgc cgtgctcgag cagggtggcc tccacggcga caccctgagc ggcaccgcgg 120
acgacgcgca ggccgtcttc gagcagctgg tgcaggtcgg catcgacatg gaggacgtct 180
tcgcggtgct ggagcgcgag ggcgtcgaga agttcgtgtc ctcctggtcc gagctgctcg 240
actccatgga gtcccgactg cgctagcagt cccctccccg acaggaaagg acctgagcgc 300
atg ccc ctc ccc gtg acc ggc acc gac gcc tgg atc aac ccg ctg cgg 348
Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg
1 5 10 15
aac acc gtc gac aag cgg ctg ccc cgc atc gcc ggc ccc tcc ggc atg 396
Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met
20 25 30
gtg atc ttc ggc gtg acc ggt gac ctg gcc cgc aag aag ctg ctc ccg 444
Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro
35 40 45
gcc atc tac gac ctg gcc aac cgt ggc ctc ctg ccg gcc ggt ttc acc 492
Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr
50 55 60
ctc atc ggt ttc ggc cgt cgt ccc tgg agc aaa ccc gag ttc gag gag 540
Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu
65 70 75 80
tac gtg cgg gag gcc gtc gag agc ggc gcc cgc acc gag ttc cgc gaa 588
Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu
85 90 95
cac gtg tgg gag cgg ctg gcc gag ggc atg cac ttc gtc gag ggc aac 636
His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn
100 105 110
ttc gac gac gag ggc ttc gac cgc ctc gcc gcc acc ctc gcc gag atg 684
Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met
115 120 125
gac acc tcc cgc ggc acg gcg ggc aac tgg gcc ttc tac ctg tcg gtc 732
Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val
130 135 140
ccg ccg gac ttc ttc acc gcc gtc tgc cac cag ctg gag cgc tcc ggc 780
Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly
145 150 155 160
ctg gcg gag gcc ggt gag gag tcg tgg cgt cgc gtg atc atc gag aag 828
Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys
165 170 175
ccc ttc ggc cac gac cag gac acg gcc cgg gag ctc aac cgg gtg gtc 876
Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val
180 185 190
aac gcc gtc ttc ccg gag gag tcg gtg ttc cgc atc gac cac tac ctg 924
Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu
195 200 205
ggc aag gag acg gtg cag aac atc ctg gcg ctg cgc ttc gcc aac cag 972
Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln
210 215 220
ctc ttc gac ccg atc tgg aac gcg cac tac gtc gac cac gtg cag atc 1020
Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile
225 230 235 240
acc atg gcc gag gac atc ggt ctc ggt ggt cgg gcc ggc tac tac gac 1068
Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp
245 250 255
ggc atc ggc gcc gcc cgc gac gtc atg cag aac cac ctc ctc cag ctc 1116
Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu
260 265 270
ctg gcg ctg gtg gcc atg gag gag ccc atc gcc ttc gac ccg gct gag 1164
Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu
275 280 285
ctg cag gcg gag aag gtc aag gtc ctg cgc gcg acc aac ccc gtc ttc 1212
Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe
290 295 300
ccg ctg aac aag acc acc gcc cgt ggc cag tac gcg gcg ggc tgg cag 1260
Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln
305 310 315 320
ggc tcg cac aag gtg cgt ggc ctg cgt gag gag gag ggt ttc gac ccg 1308
Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro
325 330 335
gac tcc acc acg gag acc tac gcc gcc tgc acc ctg gag atc aac tcc 1356
Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser
340 345 350
cgc cgc tgg gcg ggc gtg ccc ttc tac ctg cgg acc ggc aag cgc ctg 1404
Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu
355 360 365
ggg cgc cgc gtc acg gag atc gcc ctc gtg ttc aag gag gcg ccg cac 1452
Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His
370 375 380
cag ccc ttc ctc gag ggg cag acg tcc tcg ctg ggg cag aac gcg atc 1500
Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile
385 390 395 400
gtc atc cgc gtc cag ccc gac gag ggc gtg ctc atg cgc ttc ggc tcc 1548
Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser
405 410 415
aag gtg ccc ggc tcc gcg atg gag gtc cgt gac gtc aac atg gac ttc 1596
Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe
420 425 430
tcc tac gcg gag gcc ttc acc gag gcg tcc ccg gag gcc tat gag cgg 1644
Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg
435 440 445
ctc atc ctc gat gcg ctt ctc gac gag tcc tcg ctc ttc ccc acc aac 1692
Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn
450 455 460
gag gag gtc gag ctc tcc tgg gcg atc ctg gac ccg atc ctc gag ttc 1740
Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe
465 470 475 480
tgg gct gag aag ggc cag ccc gag gag tac gag tcc ggc acc tgg ggg 1788
Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly
485 490 495
ccc gcc tcc gcc gac cgc atg ctg gaa cgg acc ggc cgc gcc tgg cgc 1836
Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg
500 505 510
cgc ccc tga cctggaaggg acccgacttg aagatcgaac tgccggacac 1885
Arg Pro
cgacacccgg aacatcgccg ccagcctgct cgaggcccgg gagcacaact cccagaccac 1945
cgggcgggtg ctcaccctca tcgtcgtcgc cgcgctcgac gacgacgtcg accacatcat 2005
ctccgtcacc cgcgacgcct cccacgagca ccccgcccgt gtgctggtgc tgctcaccgg 2065
ccgggaggac gctgactcgc gtctcgacgc cgaggtgcgc gtcggcggtc acgccggcgc 2125
ctcggagatg gtcatcatgc 2145
<210> 172
<211> 514
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 172
Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg
1 5 10 15
Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met
20 25 30
Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro
35 40 45
Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr
50 55 60
Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu
65 70 75 80
Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu
85 90 95
His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn
100 105 110
Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met
115 120 125
Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val
130 135 140
Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly
145 150 155 160
Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys
165 170 175
Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val
180 185 190
Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu
195 200 205
Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln
210 215 220
Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile
225 230 235 240
Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp
245 250 255
Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu
260 265 270
Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu
275 280 285
Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe
290 295 300
Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln
305 310 315 320
Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro
325 330 335
Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser
340 345 350
Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu
355 360 365
Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His
370 375 380
Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile
385 390 395 400
Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser
405 410 415
Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe
420 425 430
Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg
435 440 445
Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn
450 455 460
Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe
465 470 475 480
Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly
485 490 495
Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg
500 505 510
Arg Pro
<210> 173
<211> 2055
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1755)
<223> ген gnd
<400> 173
aaggctgccc acggactcgg ccagcgcgca gaacacgccc ccgttgacca ggccgacggg 60
ctgccggtgc tggtccccca cctcgaggcg ggcgcggacg cggccgtcgg cgatctgcgt 120
gtagacgatc ccgaggtggg cgtcgaggcc gacgttgcgg tcgttgagct gcgcgagctg 180
cgcggcgtcg agggccttgc ctgtcccggc gaggcggaag aggtcggtga aactgtaggt 240
ctcggtcatg agtgccgact ctactgaccc ttcgaaaatg gtgtgtctaa actctgtgcc 300
atg acc tcc cct gag aat ctc gcc cag atc ggc gtc gtc gga ctc gcc 348
Met Thr Ser Pro Glu Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala
1 5 10 15
gtc atg ggt tcc aac ctg gcc cgc aac ttc gcc cgc aac ggc cac acc 396
Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly His Thr
20 25 30
gtc gcc gtg tac aac cgt tcc ccc gag aag acc cgc gac ctc atc gcc 444
Val Ala Val Tyr Asn Arg Ser Pro Glu Lys Thr Arg Asp Leu Ile Ala
35 40 45
gag cac ggt gac gag ggc acc ttc ctc gcc tcc gag acc gtc gag gac 492
Glu His Gly Asp Glu Gly Thr Phe Leu Ala Ser Glu Thr Val Glu Asp
50 55 60
ttc gtc gcc tcc ctc gag cgc ccg cgt aag gcg atc atc atg gtc cag 540
Phe Val Ala Ser Leu Glu Arg Pro Arg Lys Ala Ile Ile Met Val Gln
65 70 75 80
gcc ggc gcg gcc acc gac gcg gtg atc aac cag ctg gcc gac gcc atg 588
Ala Gly Ala Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met
85 90 95
gag gac ggc gac atc atc atc gac ggc ggc aac gcc ctc tac agc gac 636
Glu Asp Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Ser Asp
100 105 110
acc atc cgc cgg gag cgg gag atg gcc gag cgc aac cgt cac ttc gtc 684
Thr Ile Arg Arg Glu Arg Glu Met Ala Glu Arg Asn Arg His Phe Val
115 120 125
ggc gcc ggc atc tcc ggc ggc gag gag ggg gcc ctc aac ggc ccg tcg 732
Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser
130 135 140
atc atg ccg ggt ggc ccg aag gag tcc tgg gag acc ctc ggc ccg ctg 780
Ile Met Pro Gly Gly Pro Lys Glu Ser Trp Glu Thr Leu Gly Pro Leu
145 150 155 160
ctg gag tcc gtc gct gcc gtc gtc gac ggc acc ccg tgt gtc acg cac 828
Leu Glu Ser Val Ala Ala Val Val Asp Gly Thr Pro Cys Val Thr His
165 170 175
atc ggc ccg gac ggt gcg ggc cac ttc gtc aag atg gtc cac aac ggc 876
Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly
180 185 190
atc gag tac gcc gac atg cag gtc atc ggc gag gcg tac cag ctg ctg 924
Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr Gln Leu Leu
195 200 205
cgc tac ggc gcg ggc atg agc ccg gcc gag atc tcc gag gtc ttc cgc 972
Arg Tyr Gly Ala Gly Met Ser Pro Ala Glu Ile Ser Glu Val Phe Arg
210 215 220
gag tgg aac tcc ggc gac ctc gac tcc tac ctc atc gag atc acc gcc 1020
Glu Trp Asn Ser Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala
225 230 235 240
gag gtc ctc gcg cag gtc gac gcc gag acg ggg aag ccg ctc atc gac 1068
Glu Val Leu Ala Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp
245 250 255
gtc atc gtc gac gcc gcc ggc cag aag ggc acc ggc cgc tgg acc gtc 1116
Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val
260 265 270
aag gcc gcc ctc gac ctg ggc atc ccg gtc acc ggc atc ggc gag gcc 1164
Lys Ala Ala Leu Asp Leu Gly Ile Pro Val Thr Gly Ile Gly Glu Ala
275 280 285
gtc ttc gcc cgc gcc ctg tcc tcc tcc ctc gag cag cgt gcc gcc gcg 1212
Val Phe Ala Arg Ala Leu Ser Ser Ser Leu Glu Gln Arg Ala Ala Ala
290 295 300
cag ggc gtg ctg ccc gcc ggt gag ctc gcc acc ttc gag tcc ctg ggt 1260
Gln Gly Val Leu Pro Ala Gly Glu Leu Ala Thr Phe Glu Ser Leu Gly
305 310 315 320
ctg gac cgc gac gcc ttc atc gag gac gtc cgc cgc gcc ctc tac gcc 1308
Leu Asp Arg Asp Ala Phe Ile Glu Asp Val Arg Arg Ala Leu Tyr Ala
325 330 335
tcc aag ctg gtc gcc tac gcg cag ggc ttc gac gag atc cgg gcc ggc 1356
Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Arg Ala Gly
340 345 350
tcc gcc gag cac gcc tgg gac gtc gac ccc cgc gac ctg gcc acc atc 1404
Ser Ala Glu His Ala Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile
355 360 365
tgg cgg ggc ggc tgc atc atc cgc gcc aag ttc ctc aac cgc atc gtc 1452
Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val
370 375 380
gag gcc tac gac gcc gac ccg cag ctg ccg tcc ctg ctt ctc gac gcc 1500
Glu Ala Tyr Asp Ala Asp Pro Gln Leu Pro Ser Leu Leu Leu Asp Ala
385 390 395 400
tac ttc ctc gac gag ctg cgc gac ctc atc gac tcc tgg cgt cgc gtc 1548
Tyr Phe Leu Asp Glu Leu Arg Asp Leu Ile Asp Ser Trp Arg Arg Val
405 410 415
atc gtc gcc gcc acc cag ctg ggc ctg ccg gtg ccg gtg ttc gcg tcc 1596
Ile Val Ala Ala Thr Gln Leu Gly Leu Pro Val Pro Val Phe Ala Ser
420 425 430
tcc ctg tcc tac tac gac tcg ctg cgc gcc gag cgt ctg ccg gcc gcc 1644
Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala
435 440 445
gtg atc cag ggc cag cgt gac tac ttc ggt gcg cac acc tac ggg cgc 1692
Val Ile Gln Gly Gln Arg Asp Tyr Phe Gly Ala His Thr Tyr Gly Arg
450 455 460
gtc gac aag ccg ggt gcc ttc cac acc ctg tgg tcc ggg gac cgc agc 1740
Val Asp Lys Pro Gly Ala Phe His Thr Leu Trp Ser Gly Asp Arg Ser
465 470 475 480
gag atc gag gtg taa tgcccgtgtg ggcgaggatc tcccgtttcc tgccggcggc 1795
Glu Ile Glu Val
ggccatcgtg gtggtgaccg ccagggcagt gcaggaggtc cttgcccacg ggcagtgggc 1855
ggtgtggcgg ctgtcgcggc gggagacccg cctgttcgtc gaggtattcc ggacggtgct 1915
gggcctgttc ctcgtcgtcg gcctcacccg ggtggtcgcc gcgcctccct cccccacgga 1975
actggccgga tgggcgctca ccttggggtt cctgtggatc ctcaccctca cggagacgga 2035
acccggcccc gaccccgagc 2055
<210> 174
<211> 484
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 174
Met Thr Ser Pro Glu Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala
1 5 10 15
Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly His Thr
20 25 30
Val Ala Val Tyr Asn Arg Ser Pro Glu Lys Thr Arg Asp Leu Ile Ala
35 40 45
Glu His Gly Asp Glu Gly Thr Phe Leu Ala Ser Glu Thr Val Glu Asp
50 55 60
Phe Val Ala Ser Leu Glu Arg Pro Arg Lys Ala Ile Ile Met Val Gln
65 70 75 80
Ala Gly Ala Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met
85 90 95
Glu Asp Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Ser Asp
100 105 110
Thr Ile Arg Arg Glu Arg Glu Met Ala Glu Arg Asn Arg His Phe Val
115 120 125
Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser
130 135 140
Ile Met Pro Gly Gly Pro Lys Glu Ser Trp Glu Thr Leu Gly Pro Leu
145 150 155 160
Leu Glu Ser Val Ala Ala Val Val Asp Gly Thr Pro Cys Val Thr His
165 170 175
Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly
180 185 190
Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr Gln Leu Leu
195 200 205
Arg Tyr Gly Ala Gly Met Ser Pro Ala Glu Ile Ser Glu Val Phe Arg
210 215 220
Glu Trp Asn Ser Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala
225 230 235 240
Glu Val Leu Ala Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp
245 250 255
Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val
260 265 270
Lys Ala Ala Leu Asp Leu Gly Ile Pro Val Thr Gly Ile Gly Glu Ala
275 280 285
Val Phe Ala Arg Ala Leu Ser Ser Ser Leu Glu Gln Arg Ala Ala Ala
290 295 300
Gln Gly Val Leu Pro Ala Gly Glu Leu Ala Thr Phe Glu Ser Leu Gly
305 310 315 320
Leu Asp Arg Asp Ala Phe Ile Glu Asp Val Arg Arg Ala Leu Tyr Ala
325 330 335
Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Arg Ala Gly
340 345 350
Ser Ala Glu His Ala Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile
355 360 365
Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val
370 375 380
Glu Ala Tyr Asp Ala Asp Pro Gln Leu Pro Ser Leu Leu Leu Asp Ala
385 390 395 400
Tyr Phe Leu Asp Glu Leu Arg Asp Leu Ile Asp Ser Trp Arg Arg Val
405 410 415
Ile Val Ala Ala Thr Gln Leu Gly Leu Pro Val Pro Val Phe Ala Ser
420 425 430
Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala
435 440 445
Val Ile Gln Gly Gln Arg Asp Tyr Phe Gly Ala His Thr Tyr Gly Arg
450 455 460
Val Asp Lys Pro Gly Ala Phe His Thr Leu Trp Ser Gly Asp Arg Ser
465 470 475 480
Glu Ile Glu Val
<210> 175
<211> 2103
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1803)
<223> ген mqo
<400> 175
cgagaatgtc ctcataccag tcggagaagg cgggaacgct catgatgacc acactagtgg 60
gggtacgggg gcagggggcg ggggagtgag gggcgtcgat aagttcgtgt tttccggacc 120
ttcgtcacac ccccgtgaac ctaaggcgta aggttgatgt ttaccggaca aggggagcgg 180
atctgtgccg aggacgtcag agtccgcacc cggaattgtc ctgcccaccg ggagtagtgc 240
cgacacccgg tgtgggccat gactggccag aaccttcgac gacgaatgag gtaatgcaag 300
gtg tcc acc gaa aac aag aac gct ccc aag gtc act gac gag gtc gat 348
Val Ser Thr Glu Asn Lys Asn Ala Pro Lys Val Thr Asp Glu Val Asp
1 5 10 15
gtc gcc ctc gtc ggc gcg ggt gtc atg agc gcc acc ctg ggt gcg atg 396
Val Ala Leu Val Gly Ala Gly Val Met Ser Ala Thr Leu Gly Ala Met
20 25 30
atc cgc gag ctc gag ccg agc tgg tcc cag atg atc ttc gag cgc ctg 444
Ile Arg Glu Leu Glu Pro Ser Trp Ser Gln Met Ile Phe Glu Arg Leu
35 40 45
gac ggc ccc gcc cag gag tcc tcc tcc ccg tgg aac aac gcc ggt acc 492
Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr
50 55 60
ggc cac tcc gca ctc tgc gag ctg aac tac acc ccc gag gtc aac ggc 540
Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Asn Gly
65 70 75 80
aag atc gag gtg gcc aag gcc atc ggc atc aac gag aag ttc cag gtc 588
Lys Ile Glu Val Ala Lys Ala Ile Gly Ile Asn Glu Lys Phe Gln Val
85 90 95
tcc cgc cag ttc tgg tcc cac cag atc gac cac ggc gtg ctg gac gac 636
Ser Arg Gln Phe Trp Ser His Gln Ile Asp His Gly Val Leu Asp Asp
100 105 110
ccc aag gag tgg atc aac ccc gtc gcc cac ctg gcc ttc gcc cag ggc 684
Pro Lys Glu Trp Ile Asn Pro Val Ala His Leu Ala Phe Ala Gln Gly
115 120 125
gac gac cag gtc gac tac ctc aag cgc cgc cac gag gcg ctg gcc gac 732
Asp Asp Gln Val Asp Tyr Leu Lys Arg Arg His Glu Ala Leu Ala Asp
130 135 140
aac atc ctc ttc ccg ggc atg gag tgg gtc gag gac gac gcc aag ttc 780
Asn Ile Leu Phe Pro Gly Met Glu Trp Val Glu Asp Asp Ala Lys Phe
145 150 155 160
gcc gag cgc ctc ccg gtc atg tcc gag ggc cgt gac ttc gcc gcc gag 828
Ala Glu Arg Leu Pro Val Met Ser Glu Gly Arg Asp Phe Ala Ala Glu
165 170 175
aag gtc gcc tac tcc ggc acc gac atc ggc acc gac gtc aac ttc ggt 876
Lys Val Ala Tyr Ser Gly Thr Asp Ile Gly Thr Asp Val Asn Phe Gly
180 185 190
gcc ctg acc gag cag ttc ctc act gcc gcc gag gcc tcc ggc acg gag 924
Ala Leu Thr Glu Gln Phe Leu Thr Ala Ala Glu Ala Ser Gly Thr Glu
195 200 205
atc cgc tac ggc cac gag gtc aag aac atc aag aag gac ggt ggc aag 972
Ile Arg Tyr Gly His Glu Val Lys Asn Ile Lys Lys Asp Gly Gly Lys
210 215 220
tgg aag atc acc gtc aag aac gtc cac acg ggt gac acc tcc gtg gtc 1020
Trp Lys Ile Thr Val Lys Asn Val His Thr Gly Asp Thr Ser Val Val
225 230 235 240
aag gcc aag ttc gtg ttc gtc ggc gcc ggc ggc tac gcc ctc gac ctg 1068
Lys Ala Lys Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu
245 250 255
ctg cgc aag gca ggc gtc ccg gag gtc agc ggt tac gcc ggc ttc ccg 1116
Leu Arg Lys Ala Gly Val Pro Glu Val Ser Gly Tyr Ala Gly Phe Pro
260 265 270
gtg tcc ggc ctg tgg ctg cgc tgc acc aac gag gag ctg atc aac cag 1164
Val Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Asn Gln
275 280 285
cac gac gcc aag gtc tac ggc aag gcc aag gtc ggt gcc ccg ccg atg 1212
His Asp Ala Lys Val Tyr Gly Lys Ala Lys Val Gly Ala Pro Pro Met
290 295 300
tcc gtc ccg cac ctt gac acc cgc gtc atc gac ggc aag aag ggc ctc 1260
Ser Val Pro His Leu Asp Thr Arg Val Ile Asp Gly Lys Lys Gly Leu
305 310 315 320
ctg ttc ggc ccc tac ggc ggc tgg agc ccg aag ttc ctc aag gag ggt 1308
Leu Phe Gly Pro Tyr Gly Gly Trp Ser Pro Lys Phe Leu Lys Glu Gly
325 330 335
tcg tac ctc gac ctg ttc aag tcg atc cgc ctg gac aac ctc ccg tcc 1356
Ser Tyr Leu Asp Leu Phe Lys Ser Ile Arg Leu Asp Asn Leu Pro Ser
340 345 350
tac ctc ggt gtc gcc gcc cag gag ttc gcc ctg acc aag tac ctc atc 1404
Tyr Leu Gly Val Ala Ala Gln Glu Phe Ala Leu Thr Lys Tyr Leu Ile
355 360 365
acc gag gtg gcg aag gac ttc gag aag cgc ctc gtc gac ctg cgt gac 1452
Thr Glu Val Ala Lys Asp Phe Glu Lys Arg Leu Val Asp Leu Arg Asp
370 375 380
tac gtc ccg tcc gcc aag gcc gag gac tgg gag acc gtg gtc gcc ggt 1500
Tyr Val Pro Ser Ala Lys Ala Glu Asp Trp Glu Thr Val Val Ala Gly
385 390 395 400
cag cgc gtc cag gtg atc aag ccg gcc cgc gcg ccg cgc ttc ggc tcc 1548
Gln Arg Val Gln Val Ile Lys Pro Ala Arg Ala Pro Arg Phe Gly Ser
405 410 415
ctc gag ttc ggc acc gcc ctg atc aac agc acc gac ggc tcc atc gcc 1596
Leu Glu Phe Gly Thr Ala Leu Ile Asn Ser Thr Asp Gly Ser Ile Ala
420 425 430
ggc ctg ctg ggt gcc tcc ccg ggt gcc tcc atc gcc ccg gcc gcc atg 1644
Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ala Ala Met
435 440 445
ctc gag ctc gtc gag cgc tgc ttc ggt gac cgc atg atc cag tgg ggc 1692
Leu Glu Leu Val Glu Arg Cys Phe Gly Asp Arg Met Ile Gln Trp Gly
450 455 460
ccg aag ctc aag gag atg atc ccg tcc tac ggc acc aag ctg ggc gac 1740
Pro Lys Leu Lys Glu Met Ile Pro Ser Tyr Gly Thr Lys Leu Gly Asp
465 470 475 480
gac gag aag atg ttc aac gag ctc tgg gac tac acc cag aag acc ctg 1788
Asp Glu Lys Met Phe Asn Glu Leu Trp Asp Tyr Thr Gln Lys Thr Leu
485 490 495
aag ctc gag aag tag ttccactgtg agggccgcga ccaccgggtc gcggccttcg 1843
Lys Leu Glu Lys
500
ccgattccgt aggctggtgg ccatgcaacg catcgtgccc accatctggt tcaaccggaa 1903
cgccgacgag gccgccgagt tctacgccga cgtcttcccc gacacgcttg tcgacgcccg 1963
cgtcccccac cccgaatccg gcgagaccct caccgtcgac gtgctcatcg acggctaccg 2023
cgtcaccctc ctcaacggtg ggccggagtt cggcccgacc cccgcgatca gcttcttcgt 2083
caacttcgac ccctcccgca 2103
<210> 176
<211> 500
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 176
Val Ser Thr Glu Asn Lys Asn Ala Pro Lys Val Thr Asp Glu Val Asp
1 5 10 15
Val Ala Leu Val Gly Ala Gly Val Met Ser Ala Thr Leu Gly Ala Met
20 25 30
Ile Arg Glu Leu Glu Pro Ser Trp Ser Gln Met Ile Phe Glu Arg Leu
35 40 45
Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr
50 55 60
Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Asn Gly
65 70 75 80
Lys Ile Glu Val Ala Lys Ala Ile Gly Ile Asn Glu Lys Phe Gln Val
85 90 95
Ser Arg Gln Phe Trp Ser His Gln Ile Asp His Gly Val Leu Asp Asp
100 105 110
Pro Lys Glu Trp Ile Asn Pro Val Ala His Leu Ala Phe Ala Gln Gly
115 120 125
Asp Asp Gln Val Asp Tyr Leu Lys Arg Arg His Glu Ala Leu Ala Asp
130 135 140
Asn Ile Leu Phe Pro Gly Met Glu Trp Val Glu Asp Asp Ala Lys Phe
145 150 155 160
Ala Glu Arg Leu Pro Val Met Ser Glu Gly Arg Asp Phe Ala Ala Glu
165 170 175
Lys Val Ala Tyr Ser Gly Thr Asp Ile Gly Thr Asp Val Asn Phe Gly
180 185 190
Ala Leu Thr Glu Gln Phe Leu Thr Ala Ala Glu Ala Ser Gly Thr Glu
195 200 205
Ile Arg Tyr Gly His Glu Val Lys Asn Ile Lys Lys Asp Gly Gly Lys
210 215 220
Trp Lys Ile Thr Val Lys Asn Val His Thr Gly Asp Thr Ser Val Val
225 230 235 240
Lys Ala Lys Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu
245 250 255
Leu Arg Lys Ala Gly Val Pro Glu Val Ser Gly Tyr Ala Gly Phe Pro
260 265 270
Val Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Asn Gln
275 280 285
His Asp Ala Lys Val Tyr Gly Lys Ala Lys Val Gly Ala Pro Pro Met
290 295 300
Ser Val Pro His Leu Asp Thr Arg Val Ile Asp Gly Lys Lys Gly Leu
305 310 315 320
Leu Phe Gly Pro Tyr Gly Gly Trp Ser Pro Lys Phe Leu Lys Glu Gly
325 330 335
Ser Tyr Leu Asp Leu Phe Lys Ser Ile Arg Leu Asp Asn Leu Pro Ser
340 345 350
Tyr Leu Gly Val Ala Ala Gln Glu Phe Ala Leu Thr Lys Tyr Leu Ile
355 360 365
Thr Glu Val Ala Lys Asp Phe Glu Lys Arg Leu Val Asp Leu Arg Asp
370 375 380
Tyr Val Pro Ser Ala Lys Ala Glu Asp Trp Glu Thr Val Val Ala Gly
385 390 395 400
Gln Arg Val Gln Val Ile Lys Pro Ala Arg Ala Pro Arg Phe Gly Ser
405 410 415
Leu Glu Phe Gly Thr Ala Leu Ile Asn Ser Thr Asp Gly Ser Ile Ala
420 425 430
Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ala Ala Met
435 440 445
Leu Glu Leu Val Glu Arg Cys Phe Gly Asp Arg Met Ile Gln Trp Gly
450 455 460
Pro Lys Leu Lys Glu Met Ile Pro Ser Tyr Gly Thr Lys Leu Gly Asp
465 470 475 480
Asp Glu Lys Met Phe Asn Glu Leu Trp Asp Tyr Thr Gln Lys Thr Leu
485 490 495
Lys Leu Glu Lys
500
<210> 177
<211> 3357
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(3057)
<223> ген aceE
<400> 177
gtgggagatg accctcgttg ccgccccttg tgggctggtt gtgacctcgg gggtgggagc 60
cggggtttgc aatctccgcc acacacctcg cggagaggcg ggacaggcct gcgacaaggg 120
gcgatgggac gtcgataagc ggaacgccgg acccgttcat cttcgcaagc tttacaggaa 180
tgcagaacta cgttggccaa tgaaaacacg tatcctggac tggacactcg ggcctgtccg 240
gaccaccttg ccggcgggcc cgttgctcca ccacgacacc cagaaacctg gaggcacatc 300
atg gca gat gac ctg aac gcg aac ggc cgc aag ggc aac tcc cac tac 348
Met Ala Asp Asp Leu Asn Ala Asn Gly Arg Lys Gly Asn Ser His Tyr
1 5 10 15
ccg gcg atc cgc gac ggc gtc gcg tcc tac ctc aaa gac acc gac ccg 396
Pro Ala Ile Arg Asp Gly Val Ala Ser Tyr Leu Lys Asp Thr Asp Pro
20 25 30
gag gag acc cag gag tgg atg gac tcc ctc gac ggt ctc ctc gcg gag 444
Glu Glu Thr Gln Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Ala Glu
35 40 45
aag gac ccg gcc cgc gcc cgc tac ctc atg ctc cgc atg ctg gag cgc 492
Lys Asp Pro Ala Arg Ala Arg Tyr Leu Met Leu Arg Met Leu Glu Arg
50 55 60
gcg gcc ctc aag cgt gtc ccg ctg ccg aac ctg acc tcc aca gac ttc 540
Ala Ala Leu Lys Arg Val Pro Leu Pro Asn Leu Thr Ser Thr Asp Phe
65 70 75 80
gtc aac acg atc ccc acc acc atg gag ccg gag ttc ccg ggt gac gag 588
Val Asn Thr Ile Pro Thr Thr Met Glu Pro Glu Phe Pro Gly Asp Glu
85 90 95
gag atg gag aag cgc tac cgc cgc tgg atg cgc tgg aac gcc gcc atc 636
Glu Met Glu Lys Arg Tyr Arg Arg Trp Met Arg Trp Asn Ala Ala Ile
100 105 110
atg gtg cac cgc gcc cag cgc ccc ggc atc ggc gtc ggc ggc cac atc 684
Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile
115 120 125
tcc acc tac gcc tcc gcc gcc ccg ctc tac gag gtc ggc ttc aat cac 732
Ser Thr Tyr Ala Ser Ala Ala Pro Leu Tyr Glu Val Gly Phe Asn His
130 135 140
ttc tgg cgc ggc aag gac cac ccg ggc ggc ggc gac cag atc ttc ttc 780
Phe Trp Arg Gly Lys Asp His Pro Gly Gly Gly Asp Gln Ile Phe Phe
145 150 155 160
cag ggc cac gcc tcc ccc ggc atc tac gcc cgc gcc ttc ctc gag ggg 828
Gln Gly His Ala Ser Pro Gly Ile Tyr Ala Arg Ala Phe Leu Glu Gly
165 170 175
cgc ctg agc gag gac gac ctc gac ggc ttc cgc cag gag gtc tcc cgc 876
Arg Leu Ser Glu Asp Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg
180 185 190
ggt gag ggc aac ggc atc ccg tcc tac ccg cac ccg cac ggc atg cct 924
Gly Glu Gly Asn Gly Ile Pro Ser Tyr Pro His Pro His Gly Met Pro
195 200 205
gat ttc tgg gag ttc ccg acc gtc tcc atg gga ctg ggc ccg atg gac 972
Asp Phe Trp Glu Phe Pro Thr Val Ser Met Gly Leu Gly Pro Met Asp
210 215 220
gcg atc tac cag gca cgt ttc aac cgt tac ctg cac aac cgc ggc atc 1020
Ala Ile Tyr Gln Ala Arg Phe Asn Arg Tyr Leu His Asn Arg Gly Ile
225 230 235 240
aag gac acc tcc gac cag cac gtg tgg gcc ttc ctc ggc gac ggc gag 1068
Lys Asp Thr Ser Asp Gln His Val Trp Ala Phe Leu Gly Asp Gly Glu
245 250 255
atg gac gag ccg gag tcc cgc ggc ctc atc cag cac gcc gcg ctg aac 1116
Met Asp Glu Pro Glu Ser Arg Gly Leu Ile Gln His Ala Ala Leu Asn
260 265 270
aac ctg gac aac ctc acc ttc gtg gtc aac tgc aac ctg cag cgt ctc 1164
Asn Leu Asp Asn Leu Thr Phe Val Val Asn Cys Asn Leu Gln Arg Leu
275 280 285
gac ggc ccg gtc cgc ggc aac acg cag atc atc cag gag ctc gag tcc 1212
Asp Gly Pro Val Arg Gly Asn Thr Gln Ile Ile Gln Glu Leu Glu Ser
290 295 300
ttc ttc cgg ggt gcc ggc tgg tcc gtc atc aag gtc atc tgg ggc cgc 1260
Phe Phe Arg Gly Ala Gly Trp Ser Val Ile Lys Val Ile Trp Gly Arg
305 310 315 320
gag tgg gac gcc ctg ctg gag aag gac gag tcc ggc gcg ctg gtc aac 1308
Glu Trp Asp Ala Leu Leu Glu Lys Asp Glu Ser Gly Ala Leu Val Asn
325 330 335
gtc atg aac acc acc ccg gac ggt gac tac cag acc ttc aag gcc aac 1356
Val Met Asn Thr Thr Pro Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn
340 345 350
gac ggc gcc tac gtc cgc gag cac ttc ttc ggc cgc gac gag cgc acc 1404
Asp Gly Ala Tyr Val Arg Glu His Phe Phe Gly Arg Asp Glu Arg Thr
355 360 365
gcc aaa ctc gtc gag gac atg acc gac gag gag atc tgg gcc ctg ccg 1452
Ala Lys Leu Val Glu Asp Met Thr Asp Glu Glu Ile Trp Ala Leu Pro
370 375 380
cgc ggt ggc cac gac tac cgc aag gtc cac gcc gcc tac aag cgg gcg 1500
Arg Gly Gly His Asp Tyr Arg Lys Val His Ala Ala Tyr Lys Arg Ala
385 390 395 400
ctg gag acc aag gac cgc ccg acc gtc atc ctc gcg ttc acc atc aag 1548
Leu Glu Thr Lys Asp Arg Pro Thr Val Ile Leu Ala Phe Thr Ile Lys
405 410 415
ggc tac ggc ctg ggc cac aac ttc gag ggc cgc aac gcg acg cac cag 1596
Gly Tyr Gly Leu Gly His Asn Phe Glu Gly Arg Asn Ala Thr His Gln
420 425 430
atg aag aag ctc gcc gcc gag gac ctg aag aac ttc cgc gac aag cag 1644
Met Lys Lys Leu Ala Ala Glu Asp Leu Lys Asn Phe Arg Asp Lys Gln
435 440 445
tcc atc ccg atc ccg gac gag gtc ctc gag gcc gac ccg tac ctg ccg 1692
Ser Ile Pro Ile Pro Asp Glu Val Leu Glu Ala Asp Pro Tyr Leu Pro
450 455 460
ccg tac tac cac ccg ggc gag gac gcc ccg gag atc cag tac atg aag 1740
Pro Tyr Tyr His Pro Gly Glu Asp Ala Pro Glu Ile Gln Tyr Met Lys
465 470 475 480
aag cgt cgt cag gag ctg ggc ggt ttc atc ccg gag cgt cgc cag acc 1788
Lys Arg Arg Gln Glu Leu Gly Gly Phe Ile Pro Glu Arg Arg Gln Thr
485 490 495
tac acc ccg ctg cag gtc ccg gac atc gag aag ctg cgc agc gtg cgc 1836
Tyr Thr Pro Leu Gln Val Pro Asp Ile Glu Lys Leu Arg Ser Val Arg
500 505 510
aag ggc tcc ggc aag cag gag gtc gcc tcc acc atg gcg ctc gtg cgt 1884
Lys Gly Ser Gly Lys Gln Glu Val Ala Ser Thr Met Ala Leu Val Arg
515 520 525
gcc ttc aag gag ctc atg cgt gac aag gag ctg ggc cgc cgc gtc gtg 1932
Ala Phe Lys Glu Leu Met Arg Asp Lys Glu Leu Gly Arg Arg Val Val
530 535 540
ccg atc atc ccg gac gag gcc cgc acc ttc ggc ctc gac tcc tgg ttc 1980
Pro Ile Ile Pro Asp Glu Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe
545 550 555 560
ccg acc ctg aag atc tac aac ccg cac ggc cag aac tac gtg ccg gtc 2028
Pro Thr Leu Lys Ile Tyr Asn Pro His Gly Gln Asn Tyr Val Pro Val
565 570 575
gac cat gac ctc atg ctg tcc tac cgg gag gcc acc gac ggc cag atc 2076
Asp His Asp Leu Met Leu Ser Tyr Arg Glu Ala Thr Asp Gly Gln Ile
580 585 590
ctc cac gag ggc atc aac gag gcc ggt tcc gtc gcg tcc ttc atg gcc 2124
Leu His Glu Gly Ile Asn Glu Ala Gly Ser Val Ala Ser Phe Met Ala
595 600 605
gcc ggc acc tcc tac gcc acc cac ggc gaa gcg atg atc ccg ctg tac 2172
Ala Gly Thr Ser Tyr Ala Thr His Gly Glu Ala Met Ile Pro Leu Tyr
610 615 620
atc ttc tac tcg atg ttc ggt ttc cag cgc acc ggc gac ggc atc tgg 2220
Ile Phe Tyr Ser Met Phe Gly Phe Gln Arg Thr Gly Asp Gly Ile Trp
625 630 635 640
gcc gcc gcc gac cag atg gcc cgt ggc ttc ctc atc ggc gcc acc gcc 2268
Ala Ala Ala Asp Gln Met Ala Arg Gly Phe Leu Ile Gly Ala Thr Ala
645 650 655
ggc cgc acc acc ctg acg ggt gag ggc ctg cag cac atg gac ggc cac 2316
Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu Gln His Met Asp Gly His
660 665 670
tcg cag atc ctc gcc tcc acg aac ccg ggc gtc gtg tcc tac gac ccg 2364
Ser Gln Ile Leu Ala Ser Thr Asn Pro Gly Val Val Ser Tyr Asp Pro
675 680 685
gcg ttc gcc tac gag atc gcg cac ctg ctc cat gag ggc atc gac cgc 2412
Ala Phe Ala Tyr Glu Ile Ala His Leu Leu His Glu Gly Ile Asp Arg
690 695 700
atg tac ggc ccg ggc cgc ggt gag aac gtc atc tac tac ctc acc gtg 2460
Met Tyr Gly Pro Gly Arg Gly Glu Asn Val Ile Tyr Tyr Leu Thr Val
705 710 715 720
tac aac gag ccg gtc ccg cag ccg gcc gag ccg gag aac ctc gac gtc 2508
Tyr Asn Glu Pro Val Pro Gln Pro Ala Glu Pro Glu Asn Leu Asp Val
725 730 735
gag ggc ctg cac aag ggc atg tac ctg ttc tcc cgc gcg gag gag ctc 2556
Glu Gly Leu His Lys Gly Met Tyr Leu Phe Ser Arg Ala Glu Glu Leu
740 745 750
gag gtc gag gac ggc cac gag gcc tcc atc ctg gcc tcc ggc atc ggc 2604
Glu Val Glu Asp Gly His Glu Ala Ser Ile Leu Ala Ser Gly Ile Gly
755 760 765
atg cag tgg gcg ctc aag gcc cag cag atg ctg gcc gag gag tac aac 2652
Met Gln Trp Ala Leu Lys Ala Gln Gln Met Leu Ala Glu Glu Tyr Asn
770 775 780
gtg cgt gcc aac gtc ttc tcc gtc acc tcc tgg gtc gag ctg gcc cgc 2700
Val Arg Ala Asn Val Phe Ser Val Thr Ser Trp Val Glu Leu Ala Arg
785 790 795 800
gag ggt gcg gcg aag aac aag gag cag ctg cgc aac ccg gcc gca gag 2748
Glu Gly Ala Ala Lys Asn Lys Glu Gln Leu Arg Asn Pro Ala Ala Glu
805 810 815
atc gag gag gcc ttc gcc acc agg cag ctg aag cag gtc gac ggc ggc 2796
Ile Glu Glu Ala Phe Ala Thr Arg Gln Leu Lys Gln Val Asp Gly Gly
820 825 830
acc ttc gtg gcc gtc tcc gac ttc gcg acc gac ctg cag gag cag atc 2844
Thr Phe Val Ala Val Ser Asp Phe Ala Thr Asp Leu Gln Glu Gln Ile
835 840 845
cgc gcc tac gtc ccg ggc gag tac atc gtc ctg ggc gcc gac ggc ttc 2892
Arg Ala Tyr Val Pro Gly Glu Tyr Ile Val Leu Gly Ala Asp Gly Phe
850 855 860
ggc ttc tcc gac acc cgt ccg gcc gcc cgc cgc ttc ttc aac atc gac 2940
Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp
865 870 875 880
gcc gag tcc atc gtc gtc gcg gtc ctc atg ggc ctg gcc cgc gag ggc 2988
Ala Glu Ser Ile Val Val Ala Val Leu Met Gly Leu Ala Arg Glu Gly
885 890 895
aag atc gac atc tct gtg gcc cag cag gcc gcc gag aag tac cag ctg 3036
Lys Ile Asp Ile Ser Val Ala Gln Gln Ala Ala Glu Lys Tyr Gln Leu
900 905 910
gac gat ccg acc gct gtg taa ctgatccccc ggtcaaggcc ctgctccggc 3087
Asp Asp Pro Thr Ala Val
915
agggccttgc tgctttcccg cttgtcgacg cccctctcac tccctgttga ggtaccgcgg 3147
ccaggaggtg tcgcgggtgg ccagcgagat ctccaccgcg gtgtcgagct ccgctgccag 3207
gggctcgggc agcttgtgcc ggagcgcgtc gccgaggcgc gcgatgacct ccaggcgcag 3267
caggttgcgg gtctccgtga tgtagatgcg cgggtaccgg gtggccagct cgacctcgga 3327
ggggtcggcg ggcccgccgt acatctcgac 3357
<210> 178
<211> 918
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 178
Met Ala Asp Asp Leu Asn Ala Asn Gly Arg Lys Gly Asn Ser His Tyr
1 5 10 15
Pro Ala Ile Arg Asp Gly Val Ala Ser Tyr Leu Lys Asp Thr Asp Pro
20 25 30
Glu Glu Thr Gln Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Ala Glu
35 40 45
Lys Asp Pro Ala Arg Ala Arg Tyr Leu Met Leu Arg Met Leu Glu Arg
50 55 60
Ala Ala Leu Lys Arg Val Pro Leu Pro Asn Leu Thr Ser Thr Asp Phe
65 70 75 80
Val Asn Thr Ile Pro Thr Thr Met Glu Pro Glu Phe Pro Gly Asp Glu
85 90 95
Glu Met Glu Lys Arg Tyr Arg Arg Trp Met Arg Trp Asn Ala Ala Ile
100 105 110
Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile
115 120 125
Ser Thr Tyr Ala Ser Ala Ala Pro Leu Tyr Glu Val Gly Phe Asn His
130 135 140
Phe Trp Arg Gly Lys Asp His Pro Gly Gly Gly Asp Gln Ile Phe Phe
145 150 155 160
Gln Gly His Ala Ser Pro Gly Ile Tyr Ala Arg Ala Phe Leu Glu Gly
165 170 175
Arg Leu Ser Glu Asp Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg
180 185 190
Gly Glu Gly Asn Gly Ile Pro Ser Tyr Pro His Pro His Gly Met Pro
195 200 205
Asp Phe Trp Glu Phe Pro Thr Val Ser Met Gly Leu Gly Pro Met Asp
210 215 220
Ala Ile Tyr Gln Ala Arg Phe Asn Arg Tyr Leu His Asn Arg Gly Ile
225 230 235 240
Lys Asp Thr Ser Asp Gln His Val Trp Ala Phe Leu Gly Asp Gly Glu
245 250 255
Met Asp Glu Pro Glu Ser Arg Gly Leu Ile Gln His Ala Ala Leu Asn
260 265 270
Asn Leu Asp Asn Leu Thr Phe Val Val Asn Cys Asn Leu Gln Arg Leu
275 280 285
Asp Gly Pro Val Arg Gly Asn Thr Gln Ile Ile Gln Glu Leu Glu Ser
290 295 300
Phe Phe Arg Gly Ala Gly Trp Ser Val Ile Lys Val Ile Trp Gly Arg
305 310 315 320
Glu Trp Asp Ala Leu Leu Glu Lys Asp Glu Ser Gly Ala Leu Val Asn
325 330 335
Val Met Asn Thr Thr Pro Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn
340 345 350
Asp Gly Ala Tyr Val Arg Glu His Phe Phe Gly Arg Asp Glu Arg Thr
355 360 365
Ala Lys Leu Val Glu Asp Met Thr Asp Glu Glu Ile Trp Ala Leu Pro
370 375 380
Arg Gly Gly His Asp Tyr Arg Lys Val His Ala Ala Tyr Lys Arg Ala
385 390 395 400
Leu Glu Thr Lys Asp Arg Pro Thr Val Ile Leu Ala Phe Thr Ile Lys
405 410 415
Gly Tyr Gly Leu Gly His Asn Phe Glu Gly Arg Asn Ala Thr His Gln
420 425 430
Met Lys Lys Leu Ala Ala Glu Asp Leu Lys Asn Phe Arg Asp Lys Gln
435 440 445
Ser Ile Pro Ile Pro Asp Glu Val Leu Glu Ala Asp Pro Tyr Leu Pro
450 455 460
Pro Tyr Tyr His Pro Gly Glu Asp Ala Pro Glu Ile Gln Tyr Met Lys
465 470 475 480
Lys Arg Arg Gln Glu Leu Gly Gly Phe Ile Pro Glu Arg Arg Gln Thr
485 490 495
Tyr Thr Pro Leu Gln Val Pro Asp Ile Glu Lys Leu Arg Ser Val Arg
500 505 510
Lys Gly Ser Gly Lys Gln Glu Val Ala Ser Thr Met Ala Leu Val Arg
515 520 525
Ala Phe Lys Glu Leu Met Arg Asp Lys Glu Leu Gly Arg Arg Val Val
530 535 540
Pro Ile Ile Pro Asp Glu Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe
545 550 555 560
Pro Thr Leu Lys Ile Tyr Asn Pro His Gly Gln Asn Tyr Val Pro Val
565 570 575
Asp His Asp Leu Met Leu Ser Tyr Arg Glu Ala Thr Asp Gly Gln Ile
580 585 590
Leu His Glu Gly Ile Asn Glu Ala Gly Ser Val Ala Ser Phe Met Ala
595 600 605
Ala Gly Thr Ser Tyr Ala Thr His Gly Glu Ala Met Ile Pro Leu Tyr
610 615 620
Ile Phe Tyr Ser Met Phe Gly Phe Gln Arg Thr Gly Asp Gly Ile Trp
625 630 635 640
Ala Ala Ala Asp Gln Met Ala Arg Gly Phe Leu Ile Gly Ala Thr Ala
645 650 655
Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu Gln His Met Asp Gly His
660 665 670
Ser Gln Ile Leu Ala Ser Thr Asn Pro Gly Val Val Ser Tyr Asp Pro
675 680 685
Ala Phe Ala Tyr Glu Ile Ala His Leu Leu His Glu Gly Ile Asp Arg
690 695 700
Met Tyr Gly Pro Gly Arg Gly Glu Asn Val Ile Tyr Tyr Leu Thr Val
705 710 715 720
Tyr Asn Glu Pro Val Pro Gln Pro Ala Glu Pro Glu Asn Leu Asp Val
725 730 735
Glu Gly Leu His Lys Gly Met Tyr Leu Phe Ser Arg Ala Glu Glu Leu
740 745 750
Glu Val Glu Asp Gly His Glu Ala Ser Ile Leu Ala Ser Gly Ile Gly
755 760 765
Met Gln Trp Ala Leu Lys Ala Gln Gln Met Leu Ala Glu Glu Tyr Asn
770 775 780
Val Arg Ala Asn Val Phe Ser Val Thr Ser Trp Val Glu Leu Ala Arg
785 790 795 800
Glu Gly Ala Ala Lys Asn Lys Glu Gln Leu Arg Asn Pro Ala Ala Glu
805 810 815
Ile Glu Glu Ala Phe Ala Thr Arg Gln Leu Lys Gln Val Asp Gly Gly
820 825 830
Thr Phe Val Ala Val Ser Asp Phe Ala Thr Asp Leu Gln Glu Gln Ile
835 840 845
Arg Ala Tyr Val Pro Gly Glu Tyr Ile Val Leu Gly Ala Asp Gly Phe
850 855 860
Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp
865 870 875 880
Ala Glu Ser Ile Val Val Ala Val Leu Met Gly Leu Ala Arg Glu Gly
885 890 895
Lys Ile Asp Ile Ser Val Ala Gln Gln Ala Ala Glu Lys Tyr Gln Leu
900 905 910
Asp Asp Pro Thr Ala Val
915
<210> 179
<211> 1752
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1452)
<223> ген gltA
<400> 179
agctgctctc cgcccagcag ctcgaggagc tcggcttcaa cgccgtcatc tacccggtga 60
ccaccctgcg catcgccatg ggacaggtcg aggaggctct cgcggagatc gccgccaccg 120
gcacccagac cgggtgggtc gaccgcatgc agcaccgctc ccgcctctac gaactgctcc 180
gctacaacga gtacaacgcc ttcgaccagg aggttttcac gtactccgcc gacacctacc 240
agcccacctt ccagtagccc accagcaact cctgacattc cttgaaaggg gatgagtacc 300
atg tcc gac aac acc aac atc gag gtc cgc aag ggt ctc tac ggc gtg 348
Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val
1 5 10 15
atc gcc gac tac acc gca gtc tcc aag gtc atg ccg gag acc aac tcc 396
Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser
20 25 30
ctg acc tac cgc gga tac gcc gtc cag gac ctc gtc gca gag tgc tcc 444
Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser
35 40 45
ttc gag gag gtc ttc tac ctc ctg tgg aac ggt gag ctg ccg aac gag 492
Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu
50 55 60
gag cag ctg gcg gag ttc aac agg cgc ggc cgt tcc tac cgc aag ctc 540
Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu
65 70 75 80
gac gcc ggc ctg atc gca ctc atc cac tcc ctg ccg ctc gac tgt cac 588
Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His
85 90 95
ccg atg gac gtc atg cgc acc gcc gtg tcc tac atg ggc acc aag gac 636
Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp
100 105 110
tcc gag cac ttc acc ccg gac gcc gac cac atc acc cac gtc ggc cac 684
Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His
115 120 125
aac ctc ctc gcc cag ctg ccg atg gtg ctg gcc atg gac atc cgc cgc 732
Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg
130 135 140
cgt cag ggc ctc gac atc gtc gcc ccg gac ccg gac aag tcc gtc gcc 780
Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala
145 150 155 160
cac aac ctg ctg tcg atg gtc ttc ggc aac ggc ccg gag tcc ccg gcg 828
His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala
165 170 175
tcc aac ccg gac gac gtg cgt gac ttc gag aag tcg ctc atc ctc tac 876
Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr
180 185 190
gcc gag cac tcc ttc aac gcc tcg acc ttc acc tcg cgt gtg atc acc 924
Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr
195 200 205
tcg acc cgc tcg gac gtc tac tcc gcg atc acc ggt gcc atc ggc gcc 972
Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala
210 215 220
ctc aag ggc ccg ctg cac ggc ggc gcc aac gag ttc gtc atg cac acc 1020
Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr
225 230 235 240
atg ctg gag atc gac gac ccg gag aag gcc gcc gac tgg gtc aac aac 1068
Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn
245 250 255
gcc ctg gac aac aag gac ctc atc atg ggc ttc ggt cac cgc gtg tac 1116
Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr
260 265 270
aag aag ggt gac tcg cgt gtc ccg tcc atg gag gcg tcc ttc cgt gac 1164
Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp
275 280 285
ctc gcc gag cgt cac gac ggc gcc aag tgg gtc gcc atg tac gag aac 1212
Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn
290 295 300
atg cgc gac gcc atg gac gcc cgc acc ggc atc aag ccg aac ctg gac 1260
Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp
305 310 315 320
ttc ccg gcc ggc ccg gcg tac cac ctg ctc ggc ttc ccg gtc gac ttc 1308
Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe
325 330 335
ttc acc ccg ctg ttc gtc gtc gcc cgc atc gcc ggc tgg acc gcc cac 1356
Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His
340 345 350
atc gtg gag cag tac aac gac aac tcc ctg att cgt ccg ctc tcc gcc 1404
Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala
355 360 365
tac aac ggc ccc gag cag cgc cag gtc acc ccg ctg gcc gcc cgt tag 1452
Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg
370 375 380
ccccacggcc ggcaccggac caccgcgacg cccacggcac tgcccacaca ttggggtggt 1512
gcgcgtgggt ttcgctgttc ggaacacctg tcggcccatt ccgcagggcg ggacagaaac 1572
ggcctttcgg gcgcccgtcc cgcggagtgc ggaaatggac cgcaccgggt ccaccacctg 1632
cagatacccc ctgtgactag gggatactcc cccgaagaca accctctttc ttgcgatact 1692
gtggtgtgcc agagagctca cccgctctca gacacacccc cgacatgacg ttcggaaagg 1752
<210> 180
<211> 383
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 180
Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val
1 5 10 15
Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser
20 25 30
Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser
35 40 45
Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu
50 55 60
Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu
65 70 75 80
Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His
85 90 95
Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp
100 105 110
Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His
115 120 125
Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg
130 135 140
Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala
145 150 155 160
His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala
165 170 175
Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr
180 185 190
Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr
195 200 205
Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala
210 215 220
Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr
225 230 235 240
Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn
245 250 255
Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr
260 265 270
Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp
275 280 285
Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn
290 295 300
Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp
305 310 315 320
Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe
325 330 335
Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His
340 345 350
Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala
355 360 365
Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg
370 375 380
<210> 181
<211> 1578
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1278)
<223> ген mdh
<400> 181
gaacatgcgg ggtcgtccct ccgggggcac gggtcggggt ggaagggtct ggaagggggc 60
ggtgccggcg ggtctgtgcg ggccgcaacc ggcgctgtga tccatcgtgc ctgatgtatc 120
ggtcggtggt ggggcggtgc ggctgagaag atgctggacc acccgatgta gcgacgttct 180
tccagcggaa acagccggat ctccggcgag gttacgccca tccgggggtg gcggctcgga 240
acggtctttg aggctagact cgcaggcgtt gatgcattag accgctcgaa aggatcccgc 300
atg acc cag aac ccc gtg aag atc gcc gtc acc ggt gcc gcc ggc aac 348
Met Thr Gln Asn Pro Val Lys Ile Ala Val Thr Gly Ala Ala Gly Asn
1 5 10 15
atc gcc tac tcc ctc ctg tgg cgc atc gcc gca ggt gac gtc tac ggc 396
Ile Ala Tyr Ser Leu Leu Trp Arg Ile Ala Ala Gly Asp Val Tyr Gly
20 25 30
cgc gac acc ccg atc gag ctc acc ctc ctc gag atc ccg gcc gcc gta 444
Arg Asp Thr Pro Ile Glu Leu Thr Leu Leu Glu Ile Pro Ala Ala Val
35 40 45
cgc ggc gcc gag ggc gtg gcc atg gag ctc aac gac tcc gcc ttc ccc 492
Arg Gly Ala Glu Gly Val Ala Met Glu Leu Asn Asp Ser Ala Phe Pro
50 55 60
ctc ctg cgc aac atc acc gtc acc gac agc gcc gac gtc gcc ttc gac 540
Leu Leu Arg Asn Ile Thr Val Thr Asp Ser Ala Asp Val Ala Phe Asp
65 70 75 80
ggc gcc aac gcc gcc ttc ctt gtc ggc gcg aag ccg cgc ggt gca ggc 588
Gly Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Ala Gly
85 90 95
gag gag cgc gcc gac ctg ctg aag aac aac ggc ggc atc ttc ggc ccg 636
Glu Glu Arg Ala Asp Leu Leu Lys Asn Asn Gly Gly Ile Phe Gly Pro
100 105 110
cag ggc agg gcc atc aac gac cgc gcc gcg gac gac gtc cgc gtg ctc 684
Gln Gly Arg Ala Ile Asn Asp Arg Ala Ala Asp Asp Val Arg Val Leu
115 120 125
gtc gtc ggc aac ccg gcc aac acc aac gcc ctg atc gcc cag cgc aac 732
Val Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Gln Arg Asn
130 135 140
gcc ccg gac gtc ccg gcc gag cgt ttc acc gcg ctg atg cgc ctg gac 780
Ala Pro Asp Val Pro Ala Glu Arg Phe Thr Ala Leu Met Arg Leu Asp
145 150 155 160
cac agc cgt gcg ctg tcc cag ctg gcc gag aag ctc ggc gtg ctc acc 828
His Ser Arg Ala Leu Ser Gln Leu Ala Glu Lys Leu Gly Val Leu Thr
165 170 175
acc gac ttc gag aac gtc gtc gtg tgg ggc aac cac tcc gcg acc cag 876
Thr Asp Phe Glu Asn Val Val Val Trp Gly Asn His Ser Ala Thr Gln
180 185 190
ttc ccg gac gtg acc ttc gcc acc gtc aag ggt gag ccg gtc atc gag 924
Phe Pro Asp Val Thr Phe Ala Thr Val Lys Gly Glu Pro Val Ile Glu
195 200 205
aag atc gac cat gac tgg tac gtc aac gag ttc atc ccg cgc gtc gcg 972
Lys Ile Asp His Asp Trp Tyr Val Asn Glu Phe Ile Pro Arg Val Ala
210 215 220
aag cgt ggc gcc gag atc atc gag gtc cgc ggc aag tcc tcc gcc gcc 1020
Lys Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala
225 230 235 240
tcc gcc gcc tcc gcc gcg gtg gac cac atg cgt gac tgg gtc cag ggc 1068
Ser Ala Ala Ser Ala Ala Val Asp His Met Arg Asp Trp Val Gln Gly
245 250 255
act gag cgg tgg gcc tcc gcc gcc ctg ccg tcc gac ggc tcc tac ggc 1116
Thr Glu Arg Trp Ala Ser Ala Ala Leu Pro Ser Asp Gly Ser Tyr Gly
260 265 270
atc gac gag ggt ctc gtc gcc ggc ctg ccg acc gtc tcc cgc aac ggc 1164
Ile Asp Glu Gly Leu Val Ala Gly Leu Pro Thr Val Ser Arg Asn Gly
275 280 285
tcc tgg gag atc gtc cag ggc ctc gag ctc aac gac ttc cag cgc gag 1212
Ser Trp Glu Ile Val Gln Gly Leu Glu Leu Asn Asp Phe Gln Arg Glu
290 295 300
atg atc gcc cgg aac gtc gcg gag ctc aag gcc gag cgt gac gca gtc 1260
Met Ile Ala Arg Asn Val Ala Glu Leu Lys Ala Glu Arg Asp Ala Val
305 310 315 320
gct gac ctg ctt cct tag cgcgtcaact cgcatccgcc gccaatcccg 1308
Ala Asp Leu Leu Pro
325
gtagtgttca ggaacatgcg cttcgggatt gatttcggaa ccacccgtac cgtcattgcg 1368
gcggtcgacc ggggaaacta cccggtcgtc aacgtcacgg acacggacgg cgactcacgg 1428
gagtacatcc cctcggtcgt cgcgctggat ggtgaacggt tggtcggtgg ctgggaggcg 1488
ctggcggtag gtggcccgac cctcacccgc tccttcaagc gtctgctgac ctcccgcacc 1548
gtcaccgcgg agacccccgt ccggctgggc 1578
<210> 182
<211> 325
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 182
Met Thr Gln Asn Pro Val Lys Ile Ala Val Thr Gly Ala Ala Gly Asn
1 5 10 15
Ile Ala Tyr Ser Leu Leu Trp Arg Ile Ala Ala Gly Asp Val Tyr Gly
20 25 30
Arg Asp Thr Pro Ile Glu Leu Thr Leu Leu Glu Ile Pro Ala Ala Val
35 40 45
Arg Gly Ala Glu Gly Val Ala Met Glu Leu Asn Asp Ser Ala Phe Pro
50 55 60
Leu Leu Arg Asn Ile Thr Val Thr Asp Ser Ala Asp Val Ala Phe Asp
65 70 75 80
Gly Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Ala Gly
85 90 95
Glu Glu Arg Ala Asp Leu Leu Lys Asn Asn Gly Gly Ile Phe Gly Pro
100 105 110
Gln Gly Arg Ala Ile Asn Asp Arg Ala Ala Asp Asp Val Arg Val Leu
115 120 125
Val Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Gln Arg Asn
130 135 140
Ala Pro Asp Val Pro Ala Glu Arg Phe Thr Ala Leu Met Arg Leu Asp
145 150 155 160
His Ser Arg Ala Leu Ser Gln Leu Ala Glu Lys Leu Gly Val Leu Thr
165 170 175
Thr Asp Phe Glu Asn Val Val Val Trp Gly Asn His Ser Ala Thr Gln
180 185 190
Phe Pro Asp Val Thr Phe Ala Thr Val Lys Gly Glu Pro Val Ile Glu
195 200 205
Lys Ile Asp His Asp Trp Tyr Val Asn Glu Phe Ile Pro Arg Val Ala
210 215 220
Lys Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala
225 230 235 240
Ser Ala Ala Ser Ala Ala Val Asp His Met Arg Asp Trp Val Gln Gly
245 250 255
Thr Glu Arg Trp Ala Ser Ala Ala Leu Pro Ser Asp Gly Ser Tyr Gly
260 265 270
Ile Asp Glu Gly Leu Val Ala Gly Leu Pro Thr Val Ser Arg Asn Gly
275 280 285
Ser Trp Glu Ile Val Gln Gly Leu Glu Leu Asn Asp Phe Gln Arg Glu
290 295 300
Met Ile Ala Arg Asn Val Ala Glu Leu Lys Ala Glu Arg Asp Ala Val
305 310 315 320
Ala Asp Leu Leu Pro
325
<210> 183
<211> 2100
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1800)
<223> ген murE
<400> 183
gccggcaaga cgggtacggc ccagaaggtc gacccgcgga cgggcgcgta ctcgaactcg 60
gactactgga tcaccttcgc cggcatcgcc ccggcggatg acccgcgctt cgtcgtcgcc 120
gtcatggtcg acgagccccg cgccggcgtc gaggcgggag gcggcggtgg ccagtcctcg 180
gcgccgttgt tccgtgacat cgcgtcctgg ctgctcaacc gggacaacat cccggcgtca 240
ccgcccatgg aggacatgct gatcctccac gcccactaac ccagcccacc acggaggagt 300
atg acc atg gag acc aca gtg acc aca ctg tcc cac ctc gcc cgt atc 348
Met Thr Met Glu Thr Thr Val Thr Thr Leu Ser His Leu Ala Arg Ile
1 5 10 15
gcc ggg gga gag ctg acc gga tcc gtg gcc ccg gtc gtc acg tcg gtg 396
Ala Gly Gly Glu Leu Thr Gly Ser Val Ala Pro Val Val Thr Ser Val
20 25 30
gag ctc gac tcc tcc cgc ctc gcg gac gag ggc gcg ctc ttc gcc gcc 444
Glu Leu Asp Ser Ser Arg Leu Ala Asp Glu Gly Ala Leu Phe Ala Ala
35 40 45
gtc ccc ggc acg cgc cgc cac ggt gcg gag ttc gcg cgc ggc acg ccg 492
Val Pro Gly Thr Arg Arg His Gly Ala Glu Phe Ala Arg Gly Thr Pro
50 55 60
gcc gtc gcg atc ctc acc gac gcc gcc gga aag gag ctt ctc gac gcc 540
Ala Val Ala Ile Leu Thr Asp Ala Ala Gly Lys Glu Leu Leu Asp Ala
65 70 75 80
gcc ggc gac acc cgc cct gtc atc ctc gtc gat gac gtc cgc gcg gtc 588
Ala Gly Asp Thr Arg Pro Val Ile Leu Val Asp Asp Val Arg Ala Val
85 90 95
ctc ggg gcc gtg gcc gcg gag atc cac ggc cac ccc tcg gag cgt ctc 636
Leu Gly Ala Val Ala Ala Glu Ile His Gly His Pro Ser Glu Arg Leu
100 105 110
acc ctg ctc ggc atc acc ggc acc tcc ggg aag acg acc acc agc tac 684
Thr Leu Leu Gly Ile Thr Gly Thr Ser Gly Lys Thr Thr Thr Ser Tyr
115 120 125
ctg ctc gag cag ggg ctc atg gcg gcc gac ctg aag gtc ggc ctc atc 732
Leu Leu Glu Gln Gly Leu Met Ala Ala Asp Leu Lys Val Gly Leu Ile
130 135 140
ggc acg acg ggc acg cgc atc aac ggc cgt ccc gtc ccc acc gac ctc 780
Gly Thr Thr Gly Thr Arg Ile Asn Gly Arg Pro Val Pro Thr Asp Leu
145 150 155 160
acg acc ccg gag gcc acg acc ctg cag ccc ctg ttc gtg cgg atg ctc 828
Thr Thr Pro Glu Ala Thr Thr Leu Gln Pro Leu Phe Val Arg Met Leu
165 170 175
gcg gag ggc gtc acg cac gtg gtc atg gag gtc tcc tcc cac gcc ctg 876
Ala Glu Gly Val Thr His Val Val Met Glu Val Ser Ser His Ala Leu
180 185 190
gag ctg ggc cgg gtg gcg ggc acg cgt ttc gac gtc gcc ggg ttc acc 924
Glu Leu Gly Arg Val Ala Gly Thr Arg Phe Asp Val Ala Gly Phe Thr
195 200 205
aac ctc tcc cag gac cac ctc gac ttc cac ccg acg atg gag gag tac 972
Asn Leu Ser Gln Asp His Leu Asp Phe His Pro Thr Met Glu Glu Tyr
210 215 220
ttc gcg gcc aag gcc cgc ttc ttc gac ccg gcg tcg ccg ctg gcc gcg 1020
Phe Ala Ala Lys Ala Arg Phe Phe Asp Pro Ala Ser Pro Leu Ala Ala
225 230 235 240
gag cgg tcc gtc gtc tgc gtc gac gac gag tgg ggg cgg cgg atg gcc 1068
Glu Arg Ser Val Val Cys Val Asp Asp Glu Trp Gly Arg Arg Met Ala
245 250 255
gac ctg gcc tcg gac ccg gtg acc gtc gcg acc cgc ggc gcg gag gcc 1116
Asp Leu Ala Ser Asp Pro Val Thr Val Ala Thr Arg Gly Ala Glu Ala
260 265 270
gac gtc acc gcc gtc cag agc tcg ctc acg ccc acc ggc gcc cag gtc 1164
Asp Val Thr Ala Val Gln Ser Ser Leu Thr Pro Thr Gly Ala Gln Val
275 280 285
atc cgg ctc acc ctg ccg gag ggg gcc gtc gag gcg gag ctg ccg ctg 1212
Ile Arg Leu Thr Leu Pro Glu Gly Ala Val Glu Ala Glu Leu Pro Leu
290 295 300
ccg ggt gac ttc aac atc gcc aac gcc gcc ctg gcc acc gcc atg gcg 1260
Pro Gly Asp Phe Asn Ile Ala Asn Ala Ala Leu Ala Thr Ala Met Ala
305 310 315 320
tgg gcc gtg ggc gtc gac ccg cag gcc ttc acc gcc ggt ttc ctc gac 1308
Trp Ala Val Gly Val Asp Pro Gln Ala Phe Thr Ala Gly Phe Leu Asp
325 330 335
gtc gcg gtc ccc ggg cgc atg gag cgt atc gac gag ggc cag gac ttc 1356
Val Ala Val Pro Gly Arg Met Glu Arg Ile Asp Glu Gly Gln Asp Phe
340 345 350
ctc gcc gtc gtc gac tac gcc cac aag ccg gcc gcc gtc gcc gcc gtc 1404
Leu Ala Val Val Asp Tyr Ala His Lys Pro Ala Ala Val Ala Ala Val
355 360 365
ctc gac acc ctc cgc ggg cag gtc cgc ggc cgc ctc ggc gtg gtc gtc 1452
Leu Asp Thr Leu Arg Gly Gln Val Arg Gly Arg Leu Gly Val Val Val
370 375 380
ggg gcc ggt ggt gac cgc gac gcc acc aag cgt ccc ctc atg ggg gcg 1500
Gly Ala Gly Gly Asp Arg Asp Ala Thr Lys Arg Pro Leu Met Gly Ala
385 390 395 400
gag gcc gcc cac cgc gcc gac ctg gtc atc atc acc gac gac aac ccg 1548
Glu Ala Ala His Arg Ala Asp Leu Val Ile Ile Thr Asp Asp Asn Pro
405 410 415
cgc agc gag gtg ccc gcc acc atc cgg gcg gcc gtg ctg cag ggg gcg 1596
Arg Ser Glu Val Pro Ala Thr Ile Arg Ala Ala Val Leu Gln Gly Ala
420 425 430
gtc gac gcc ggg acg gac gcg gag atc cgc gag atc ggg gac cgc gcc 1644
Val Asp Ala Gly Thr Asp Ala Glu Ile Arg Glu Ile Gly Asp Arg Ala
435 440 445
gcc gcc atc gac gcg ctg atc gag tgg gcc cgc ccg ggg gac ggc gtc 1692
Ala Ala Ile Asp Ala Leu Ile Glu Trp Ala Arg Pro Gly Asp Gly Val
450 455 460
gtc gtc gcc ggc aag ggc cac gag gtc ggc cag ctg gtc aac ggc gtc 1740
Val Val Ala Gly Lys Gly His Glu Val Gly Gln Leu Val Asn Gly Val
465 470 475 480
aac cac cat ttc gac gac cgg gaa gag gtc cgg cgc gca ctc aag gag 1788
Asn His His Phe Asp Asp Arg Glu Glu Val Arg Arg Ala Leu Lys Glu
485 490 495
aag aag cat tga tccccctcac tctcgcggag atcgcggaca tcaccggcgg 1840
Lys Lys His
tgaactctcg cacgtgaccg acccggagac ccgggtgacc gggtccgtcg agttcgactc 1900
ccgccgggtc accccgggcg ggctgttcct cgccctgccg ggggcgcgcg tcgacggaca 1960
cgacttcgcc gccaccgccg tcgagtccgg cgcggtcgcc gtcctggcgg cgcggccggt 2020
gggggtgccg gcggtcgtcg tgaagccgcg gggccgcagt gactccaacg ccgacatcta 2080
cgcccacgac gaggacggct 2100
<210> 184
<211> 499
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 184
Met Thr Met Glu Thr Thr Val Thr Thr Leu Ser His Leu Ala Arg Ile
1 5 10 15
Ala Gly Gly Glu Leu Thr Gly Ser Val Ala Pro Val Val Thr Ser Val
20 25 30
Glu Leu Asp Ser Ser Arg Leu Ala Asp Glu Gly Ala Leu Phe Ala Ala
35 40 45
Val Pro Gly Thr Arg Arg His Gly Ala Glu Phe Ala Arg Gly Thr Pro
50 55 60
Ala Val Ala Ile Leu Thr Asp Ala Ala Gly Lys Glu Leu Leu Asp Ala
65 70 75 80
Ala Gly Asp Thr Arg Pro Val Ile Leu Val Asp Asp Val Arg Ala Val
85 90 95
Leu Gly Ala Val Ala Ala Glu Ile His Gly His Pro Ser Glu Arg Leu
100 105 110
Thr Leu Leu Gly Ile Thr Gly Thr Ser Gly Lys Thr Thr Thr Ser Tyr
115 120 125
Leu Leu Glu Gln Gly Leu Met Ala Ala Asp Leu Lys Val Gly Leu Ile
130 135 140
Gly Thr Thr Gly Thr Arg Ile Asn Gly Arg Pro Val Pro Thr Asp Leu
145 150 155 160
Thr Thr Pro Glu Ala Thr Thr Leu Gln Pro Leu Phe Val Arg Met Leu
165 170 175
Ala Glu Gly Val Thr His Val Val Met Glu Val Ser Ser His Ala Leu
180 185 190
Glu Leu Gly Arg Val Ala Gly Thr Arg Phe Asp Val Ala Gly Phe Thr
195 200 205
Asn Leu Ser Gln Asp His Leu Asp Phe His Pro Thr Met Glu Glu Tyr
210 215 220
Phe Ala Ala Lys Ala Arg Phe Phe Asp Pro Ala Ser Pro Leu Ala Ala
225 230 235 240
Glu Arg Ser Val Val Cys Val Asp Asp Glu Trp Gly Arg Arg Met Ala
245 250 255
Asp Leu Ala Ser Asp Pro Val Thr Val Ala Thr Arg Gly Ala Glu Ala
260 265 270
Asp Val Thr Ala Val Gln Ser Ser Leu Thr Pro Thr Gly Ala Gln Val
275 280 285
Ile Arg Leu Thr Leu Pro Glu Gly Ala Val Glu Ala Glu Leu Pro Leu
290 295 300
Pro Gly Asp Phe Asn Ile Ala Asn Ala Ala Leu Ala Thr Ala Met Ala
305 310 315 320
Trp Ala Val Gly Val Asp Pro Gln Ala Phe Thr Ala Gly Phe Leu Asp
325 330 335
Val Ala Val Pro Gly Arg Met Glu Arg Ile Asp Glu Gly Gln Asp Phe
340 345 350
Leu Ala Val Val Asp Tyr Ala His Lys Pro Ala Ala Val Ala Ala Val
355 360 365
Leu Asp Thr Leu Arg Gly Gln Val Arg Gly Arg Leu Gly Val Val Val
370 375 380
Gly Ala Gly Gly Asp Arg Asp Ala Thr Lys Arg Pro Leu Met Gly Ala
385 390 395 400
Glu Ala Ala His Arg Ala Asp Leu Val Ile Ile Thr Asp Asp Asn Pro
405 410 415
Arg Ser Glu Val Pro Ala Thr Ile Arg Ala Ala Val Leu Gln Gly Ala
420 425 430
Val Asp Ala Gly Thr Asp Ala Glu Ile Arg Glu Ile Gly Asp Arg Ala
435 440 445
Ala Ala Ile Asp Ala Leu Ile Glu Trp Ala Arg Pro Gly Asp Gly Val
450 455 460
Val Val Ala Gly Lys Gly His Glu Val Gly Gln Leu Val Asn Gly Val
465 470 475 480
Asn His His Phe Asp Asp Arg Glu Glu Val Arg Arg Ala Leu Lys Glu
485 490 495
Lys Lys His
<210> 185
<211> 2145
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1845)
<223> ген zwf
<400> 185
cgaccctcta cgtcaccgag ctggccggcc cggagaccgt caacaccatg ccggagccga 60
ccatcgacgc cgtgctcgag cagggtggcc tccacggcga caccctgagc ggcaccgcgg 120
acgacgcgca ggccgtcttc gagcagctgg tgcaggtcgg catcgacatg gaggacgtct 180
tcgcggtgct ggagcgcgag ggcgtcgaga agttcgtgtc ctcctggtcc gagctgctcg 240
actccatgga gtcccgactg cgctagcagt cccctccccg acaggaaagg acctgagcgc 300
atg ccc ctc ccc gtg acc ggc acc gac gcc tgg atc aac ccg ctg cgg 348
Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg
1 5 10 15
aac acc gtc gac aag cgg ctg ccc cgc atc gcc ggc ccc tcc ggc atg 396
Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met
20 25 30
gtg atc ttc ggc gtg acc ggt gac ctg gcc cgc aag aag ctg ctc ccg 444
Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro
35 40 45
gcc atc tac gac ctg gcc aac cgt ggc ctc ctg ccg gcc ggt ttc acc 492
Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr
50 55 60
ctc atc ggt ttc ggc cgt cgt ccc tgg agc aaa ccc gag ttc gag gag 540
Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu
65 70 75 80
tac gtg cgg gag gcc gtc gag agc ggc gcc cgc acc gag ttc cgc gaa 588
Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu
85 90 95
cac gtg tgg gag cgg ctg gcc gag ggc atg cac ttc gtc gag ggc aac 636
His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn
100 105 110
ttc gac gac gag ggc ttc gac cgc ctc gcc gcc acc ctc gcc gag atg 684
Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met
115 120 125
gac acc tcc cgc ggc acg gcg ggc aac tgg gcc ttc tac ctg tcg gtc 732
Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val
130 135 140
ccg ccg gac ttc ttc acc gcc gtc tgc cac cag ctg gag cgc tcc ggc 780
Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly
145 150 155 160
ctg gcg gag gcc ggt gag gag tcg tgg cgt cgc gtg atc atc gag aag 828
Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys
165 170 175
ccc ttc ggc cac gac cag gac acg gcc cgg gag ctc aac cgg gtg gtc 876
Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val
180 185 190
aac gcc gtc ttc ccg gag gag tcg gtg ttc cgc atc gac cac tac ctg 924
Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu
195 200 205
ggc aag gag acg gtg cag aac atc ctg gcg ctg cgc ttc gcc aac cag 972
Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln
210 215 220
ctc ttc gac ccg atc tgg aac gcg cac tac gtc gac cac gtg cag atc 1020
Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile
225 230 235 240
acc atg gcc gag gac atc ggt ctc ggt ggt cgg gcc ggc tac tac gac 1068
Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp
245 250 255
ggc atc ggc gcc gcc cgc gac gtc atg cag aac cac ctc ctc cag ctc 1116
Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu
260 265 270
ctg gcg ctg gtg gcc atg gag gag ccc atc gcc ttc gac ccg gct gag 1164
Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu
275 280 285
ctg cag gcg gag aag gtc aag gtc ctg cgc gcg acc aac ccc gtc ttc 1212
Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe
290 295 300
ccg ctg aac aag acc acc gcc cgt ggc cag tac gcg gcg ggc tgg cag 1260
Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln
305 310 315 320
ggc tcg cac aag gtg cgt ggc ctg cgt gag gag gag ggt ttc gac ccg 1308
Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro
325 330 335
gac tcc acc acg gag acc tac gcc gcc tgc acc ctg gag atc aac tcc 1356
Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser
340 345 350
cgc cgc tgg gcg ggc gtg ccc ttc tac ctg cgg acc ggc aag cgc ctg 1404
Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu
355 360 365
ggg cgc cgc gtc acg gag atc gcc ctc gtg ttc aag gag gcg ccg cac 1452
Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His
370 375 380
cag ccc ttc ctc gag ggg cag acg tcc tcg ctg ggg cag aac gcg atc 1500
Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile
385 390 395 400
gtc atc cgc gtc cag ccc gac gag ggc gtg ctc atg cgc ttc ggc tcc 1548
Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser
405 410 415
aag gtg ccc ggc tcc gcg atg gag gtc cgt gac gtc aac atg gac ttc 1596
Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe
420 425 430
tcc tac gcg gag gcc ttc acc gag gcg tcc ccg gag gcc tat gag cgg 1644
Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg
435 440 445
ctc atc ctc gat gcg ctt ctc gac gag tcc tcg ctc ttc ccc acc aac 1692
Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn
450 455 460
gag gag gtc gag ctc tcc tgg gcg atc ctg gac ccg atc ctc gag ttc 1740
Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe
465 470 475 480
tgg gct gag aag ggc cag ccc gag gag tac gag tcc ggc acc tgg ggg 1788
Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly
485 490 495
ccc gcc tcc gcc gac cgc atg ctg gaa cgg acc ggc cgc gcc tgg cgc 1836
Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg
500 505 510
cgc ccc tga cctggaaggg acccgacttg aagatcgaac tgccggacac 1885
Arg Pro
cgacacccgg aacatcgccg ccagcctgct cgaggcccgg gagcacaact cccagaccac 1945
cgggcgggtg ctcaccctca tcgtcgtcgc cgcgctcgac gacgacgtcg accacatcat 2005
ctccgtcacc cgcgacgcct cccacgagca ccccgcccgt gtgctggtgc tgctcaccgg 2065
ccgggaggac gctgactcgc gtctcgacgc cgaggtgcgc gtcggcggtc acgccggcgc 2125
ctcggagatg gtcatcatgc 2145
<210> 186
<211> 514
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 186
Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg
1 5 10 15
Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met
20 25 30
Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro
35 40 45
Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr
50 55 60
Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu
65 70 75 80
Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu
85 90 95
His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn
100 105 110
Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met
115 120 125
Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val
130 135 140
Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly
145 150 155 160
Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys
165 170 175
Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val
180 185 190
Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu
195 200 205
Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln
210 215 220
Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile
225 230 235 240
Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp
245 250 255
Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu
260 265 270
Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu
275 280 285
Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe
290 295 300
Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln
305 310 315 320
Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro
325 330 335
Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser
340 345 350
Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu
355 360 365
Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His
370 375 380
Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile
385 390 395 400
Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser
405 410 415
Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe
420 425 430
Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg
435 440 445
Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn
450 455 460
Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe
465 470 475 480
Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly
485 490 495
Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg
500 505 510
Arg Pro
<210> 187
<211> 1551
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1251)
<223> ген opcA
<400> 187
cgcgatggag gtccgtgacg tcaacatgga cttctcctac gcggaggcct tcaccgaggc 60
gtccccggag gcctatgagc ggctcatcct cgatgcgctt ctcgacgagt cctcgctctt 120
ccccaccaac gaggaggtcg agctctcctg ggcgatcctg gacccgatcc tcgagttctg 180
ggctgagaag ggccagcccg aggagtacga gtccggcacc tgggggcccg cctccgccga 240
ccgcatgctg gaacggaccg gccgcgcctg gcgccgcccc tgacctggaa gggacccgac 300
ttg aag atc gaa ctg ccg gac acc gac acc cgg aac atc gcc gcc agc 348
Leu Lys Ile Glu Leu Pro Asp Thr Asp Thr Arg Asn Ile Ala Ala Ser
1 5 10 15
ctg ctc gag gcc cgg gag cac aac tcc cag acc acc ggg cgg gtg ctc 396
Leu Leu Glu Ala Arg Glu His Asn Ser Gln Thr Thr Gly Arg Val Leu
20 25 30
acc ctc atc gtc gtc gcc gcg ctc gac gac gac gtc gac cac atc atc 444
Thr Leu Ile Val Val Ala Ala Leu Asp Asp Asp Val Asp His Ile Ile
35 40 45
tcc gtc acc cgc gac gcc tcc cac gag cac ccc gcc cgt gtg ctg gtg 492
Ser Val Thr Arg Asp Ala Ser His Glu His Pro Ala Arg Val Leu Val
50 55 60
ctg ctc acc ggc cgg gag gac gct gac tcg cgt ctc gac gcc gag gtg 540
Leu Leu Thr Gly Arg Glu Asp Ala Asp Ser Arg Leu Asp Ala Glu Val
65 70 75 80
cgc gtc ggc ggt cac gcc ggc gcc tcg gag atg gtc atc atg cgc atc 588
Arg Val Gly Gly His Ala Gly Ala Ser Glu Met Val Ile Met Arg Ile
85 90 95
acg ggc gag atg gtc aac cac ctc gcc gcg gtg gtc acc ccc ctg ctg 636
Thr Gly Glu Met Val Asn His Leu Ala Ala Val Val Thr Pro Leu Leu
100 105 110
ctg ccg gac acc ccg gtc gtc gcc tgg tgg ccg acc acc gcg ccg gag 684
Leu Pro Asp Thr Pro Val Val Ala Trp Trp Pro Thr Thr Ala Pro Glu
115 120 125
aac ccc tcc ggg cat ccg ctg ggc cag ctg gcg cag cgc cgc atc acc 732
Asn Pro Ser Gly His Pro Leu Gly Gln Leu Ala Gln Arg Arg Ile Thr
130 135 140
aac gcg gac cac ccg gac agt gag tcg gtc ctc cgg ctc cgc cgt gcc 780
Asn Ala Asp His Pro Asp Ser Glu Ser Val Leu Arg Leu Arg Arg Ala
145 150 155 160
acc tac gcc ccc ggt gac tcc gac atg gtg tgg tcg aag atc acc gcc 828
Thr Tyr Ala Pro Gly Asp Ser Asp Met Val Trp Ser Lys Ile Thr Ala
165 170 175
tgg cgc ggt atc gtc gcc tcc tcc ctg gac cgt cac ccg cac gag gac 876
Trp Arg Gly Ile Val Ala Ser Ser Leu Asp Arg His Pro His Glu Asp
180 185 190
gtg gag gcc gcc gtc ctc acc ggc ccg gcg gat gag ccg cag atc gac 924
Val Glu Ala Ala Val Leu Thr Gly Pro Ala Asp Glu Pro Gln Ile Asp
195 200 205
atc gcc gcc ggc tgg ctc gcc gac cgc ctc ggt gtg ccg gtc cgc cgg 972
Ile Ala Ala Gly Trp Leu Ala Asp Arg Leu Gly Val Pro Val Arg Arg
210 215 220
gag gtg tgt gaa ccg ggt aac gac gcc cgg ctc ttc ccc atc cgt cgt 1020
Glu Val Cys Glu Pro Gly Asn Asp Ala Arg Leu Phe Pro Ile Arg Arg
225 230 235 240
ctc aag ttc gaa cgc gcc tcc ggc ccg gtc cgc gtc gag gtg ctc gac 1068
Leu Lys Phe Glu Arg Ala Ser Gly Pro Val Arg Val Glu Val Leu Asp
245 250 255
cac cgg acc gtg ctc gtc gcc ctg cca ggg cgc ccg gag tcg ctc gtc 1116
His Arg Thr Val Leu Val Ala Leu Pro Gly Arg Pro Glu Ser Leu Val
260 265 270
gcc atg aac gag cgt tcc gac gcc gac tgc ctc tcc gag gaa ctg cgt 1164
Ala Met Asn Glu Arg Ser Asp Ala Asp Cys Leu Ser Glu Glu Leu Arg
275 280 285
cac ctc gac ccc gat ctc gcc tac gcc aac gct ctg cgc gga ctc acc 1212
His Leu Asp Pro Asp Leu Ala Tyr Ala Asn Ala Leu Arg Gly Leu Thr
290 295 300
cgt gtg aaa gga atc aat ggt cac cct gcg tcg tcc tga caccctgtcc 1261
Arg Val Lys Gly Ile Asn Gly His Pro Ala Ser Ser
305 310 315
accctgctcg accaggccgc cacgtcgctc atcgacgtcg tcgctgccct ccagaccacc 1321
gggggcggtg tccacgccga cggtgtggcc cgcctggtgc tcaccggcgg cggcgccggc 1381
atcggcctgc tggagcgcgt ccgtgagctc gaggacgccg actcccgcat cgactggtcc 1441
cgcgtccacg tcttcttcgg cgacgagcgc aacgtgcccg tctcccacga ggactccaac 1501
gaggggcagg cgcgtaccgc cctgctcagc cacgtcgaca tcccggaggc 1551
<210> 188
<211> 316
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 188
Leu Lys Ile Glu Leu Pro Asp Thr Asp Thr Arg Asn Ile Ala Ala Ser
1 5 10 15
Leu Leu Glu Ala Arg Glu His Asn Ser Gln Thr Thr Gly Arg Val Leu
20 25 30
Thr Leu Ile Val Val Ala Ala Leu Asp Asp Asp Val Asp His Ile Ile
35 40 45
Ser Val Thr Arg Asp Ala Ser His Glu His Pro Ala Arg Val Leu Val
50 55 60
Leu Leu Thr Gly Arg Glu Asp Ala Asp Ser Arg Leu Asp Ala Glu Val
65 70 75 80
Arg Val Gly Gly His Ala Gly Ala Ser Glu Met Val Ile Met Arg Ile
85 90 95
Thr Gly Glu Met Val Asn His Leu Ala Ala Val Val Thr Pro Leu Leu
100 105 110
Leu Pro Asp Thr Pro Val Val Ala Trp Trp Pro Thr Thr Ala Pro Glu
115 120 125
Asn Pro Ser Gly His Pro Leu Gly Gln Leu Ala Gln Arg Arg Ile Thr
130 135 140
Asn Ala Asp His Pro Asp Ser Glu Ser Val Leu Arg Leu Arg Arg Ala
145 150 155 160
Thr Tyr Ala Pro Gly Asp Ser Asp Met Val Trp Ser Lys Ile Thr Ala
165 170 175
Trp Arg Gly Ile Val Ala Ser Ser Leu Asp Arg His Pro His Glu Asp
180 185 190
Val Glu Ala Ala Val Leu Thr Gly Pro Ala Asp Glu Pro Gln Ile Asp
195 200 205
Ile Ala Ala Gly Trp Leu Ala Asp Arg Leu Gly Val Pro Val Arg Arg
210 215 220
Glu Val Cys Glu Pro Gly Asn Asp Ala Arg Leu Phe Pro Ile Arg Arg
225 230 235 240
Leu Lys Phe Glu Arg Ala Ser Gly Pro Val Arg Val Glu Val Leu Asp
245 250 255
His Arg Thr Val Leu Val Ala Leu Pro Gly Arg Pro Glu Ser Leu Val
260 265 270
Ala Met Asn Glu Arg Ser Asp Ala Asp Cys Leu Ser Glu Glu Leu Arg
275 280 285
His Leu Asp Pro Asp Leu Ala Tyr Ala Asn Ala Leu Arg Gly Leu Thr
290 295 300
Arg Val Lys Gly Ile Asn Gly His Pro Ala Ser Ser
305 310 315
<210> 189
<211> 2283
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1983)
<223> ген hutU (уроканатгидратазы)
<400> 189
gctacctgtc cccggagatc gaggagtcgg tgcagctgct cacctccggg gcgctgctgg 60
aggcggtgga gggggtcgtc ggaaagctgg catgagcggg cgaatctcag atatgagact 120
aaaggggcct ccgggccctc gatcccgact gtgacctgtc ttaccgtcag aagtgtcacc 180
gacaccggcc acaccagccc ggtgctccac acccgacgat gacagccccg ggccacccgg 240
atcgcgggtg ccccggggcc ccggcctgat cggcccccac cgtagaagga gacccacccc 300
atg acc gag tcc ccg cgc acc atc cgt gcc ccg cac ggc acc gag ctc 348
Met Thr Glu Ser Pro Arg Thr Ile Arg Ala Pro His Gly Thr Glu Leu
1 5 10 15
agc gcc aag agc tgg caa acc gag gca ccc ctg cgc atg ctc atg aac 396
Ser Ala Lys Ser Trp Gln Thr Glu Ala Pro Leu Arg Met Leu Met Asn
20 25 30
aac ctc gac ccg gag gtc gcc gag cgc ccc gag gac ctc gtc gtc tac 444
Asn Leu Asp Pro Glu Val Ala Glu Arg Pro Glu Asp Leu Val Val Tyr
35 40 45
ggc ggc acc ggc cgc gcg gca cgc agc tgg gag gcc tac gac gcg atc 492
Gly Gly Thr Gly Arg Ala Ala Arg Ser Trp Glu Ala Tyr Asp Ala Ile
50 55 60
atc gag acg ctc agg gac ctc gag gag gac gag acc ctg ctc gtc cag 540
Ile Glu Thr Leu Arg Asp Leu Glu Glu Asp Glu Thr Leu Leu Val Gln
65 70 75 80
tcc ggc aag ccc gtc ggc gtc ttc cgc acc aac gtc tgg gca ccc cgc 588
Ser Gly Lys Pro Val Gly Val Phe Arg Thr Asn Val Trp Ala Pro Arg
85 90 95
gtg ctc atc gcc aac tcc aac ctc gtc ggc gac tgg gcc aac tgg ccc 636
Val Leu Ile Ala Asn Ser Asn Leu Val Gly Asp Trp Ala Asn Trp Pro
100 105 110
gag ttc cgc cgc ctc gag gcc gag ggc ctc atg atg tac ggc cag atg 684
Glu Phe Arg Arg Leu Glu Ala Glu Gly Leu Met Met Tyr Gly Gln Met
115 120 125
acc gcc ggt tcc tgg atc tac atc gcc acc cag ggc atc ctc cag ggc 732
Thr Ala Gly Ser Trp Ile Tyr Ile Ala Thr Gln Gly Ile Leu Gln Gly
130 135 140
acc tac gag acc ttc gcc gcc gtg gcc cgc aag cgt ttc ggc ggc acc 780
Thr Tyr Glu Thr Phe Ala Ala Val Ala Arg Lys Arg Phe Gly Gly Thr
145 150 155 160
ctc gcc ggc acc ctc acc ctc acc ggc ggc tgc ggc ggc atg ggc ggg 828
Leu Ala Gly Thr Leu Thr Leu Thr Gly Gly Cys Gly Gly Met Gly Gly
165 170 175
gcc cag ccg ctg tcc gtc acc ctc aac ggc ggt gtc tgc ctc atc gtc 876
Ala Gln Pro Leu Ser Val Thr Leu Asn Gly Gly Val Cys Leu Ile Val
180 185 190
gac gtc gac gag tcc cgg ctc agg cgt cgg cag gcc aag cgc tac ctc 924
Asp Val Asp Glu Ser Arg Leu Arg Arg Arg Gln Ala Lys Arg Tyr Leu
195 200 205
gat gag gtc gcc acc gat ctc gac gac gcc gtc gcc cgc gtc agc gcc 972
Asp Glu Val Ala Thr Asp Leu Asp Asp Ala Val Ala Arg Val Ser Ala
210 215 220
gcc aag gag gag aag cgt gcc ctg tcc gtc ggc cta gtg ggc aat gcc 1020
Ala Lys Glu Glu Lys Arg Ala Leu Ser Val Gly Leu Val Gly Asn Ala
225 230 235 240
gcc gag gtg ttc ccc gag ctg ctg cgc cgc cac cgc gcc ggg gag atc 1068
Ala Glu Val Phe Pro Glu Leu Leu Arg Arg His Arg Ala Gly Glu Ile
245 250 255
acc gtc gac gtg gtc acc gac cag acc tcc gcc cac gac ccg ctc agc 1116
Thr Val Asp Val Val Thr Asp Gln Thr Ser Ala His Asp Pro Leu Ser
260 265 270
tac ctg ccc acc gag atc acc gtc gag gac tgg cag cgc gag gcc gcc 1164
Tyr Leu Pro Thr Glu Ile Thr Val Glu Asp Trp Gln Arg Glu Ala Ala
275 280 285
gag gac ccc atc acc ttc acc aag aag gca cgc gag tcc atg gcc gtg 1212
Glu Asp Pro Ile Thr Phe Thr Lys Lys Ala Arg Glu Ser Met Ala Val
290 295 300
cag gtc cag gcc atg gtc gag ttc cag gac gag ggc gcc gag gtc ttc 1260
Gln Val Gln Ala Met Val Glu Phe Gln Asp Glu Gly Ala Glu Val Phe
305 310 315 320
gac tac ggc aac tcc atc cgc gat gag gcc cgc cac gcc ggc tac tcc 1308
Asp Tyr Gly Asn Ser Ile Arg Asp Glu Ala Arg His Ala Gly Tyr Ser
325 330 335
cgc gcc ttc gag ttc ccc ggc ttc gtc ccc gcc tac atc cgg ccc ctg 1356
Arg Ala Phe Glu Phe Pro Gly Phe Val Pro Ala Tyr Ile Arg Pro Leu
340 345 350
ttc tgc gag ggt ctc ggc ccc ttc cgc tgg gtg gcc ctt tcc ggc gac 1404
Phe Cys Glu Gly Leu Gly Pro Phe Arg Trp Val Ala Leu Ser Gly Asp
355 360 365
ccg gag gac atc cgc gtc acc gac gag gcc gtc aag gag ctg ttc ccc 1452
Pro Glu Asp Ile Arg Val Thr Asp Glu Ala Val Lys Glu Leu Phe Pro
370 375 380
gac aac gag cac ctc cac cgc tgg atc gac gcg gca ggg gag tac gtc 1500
Asp Asn Glu His Leu His Arg Trp Ile Asp Ala Ala Gly Glu Tyr Val
385 390 395 400
gag ttc gag ggc ctg ccg gcc cgc atc tgc tgg ctc ggc tac ggc gag 1548
Glu Phe Glu Gly Leu Pro Ala Arg Ile Cys Trp Leu Gly Tyr Gly Glu
405 410 415
cgg cac aag gcg gga ctg ctg ttc aac cgt ctc gtc gcc gag ggc aag 1596
Arg His Lys Ala Gly Leu Leu Phe Asn Arg Leu Val Ala Glu Gly Lys
420 425 430
gtg aag gcg ccc atc gtc atc ggc cgt gac cac ctc gac tcc ggt tcc 1644
Val Lys Ala Pro Ile Val Ile Gly Arg Asp His Leu Asp Ser Gly Ser
435 440 445
gtc gcc tcc ccc tac cgg gag acc gag tcc atg ctc gac ggc tcc gac 1692
Val Ala Ser Pro Tyr Arg Glu Thr Glu Ser Met Leu Asp Gly Ser Asp
450 455 460
gcc atc gcc gac tgg ccg ctg ctc aac gcc ctc acc gcc acc tcc tcc 1740
Ala Ile Ala Asp Trp Pro Leu Leu Asn Ala Leu Thr Ala Thr Ser Ser
465 470 475 480
ggc gcg acc tgg gtc tcc ctg cac cac gga ggc ggc gtg ggc atc gga 1788
Gly Ala Thr Trp Val Ser Leu His His Gly Gly Gly Val Gly Ile Gly
485 490 495
cgg tcc atc cac gcg ggc cag gtg tgc gtc gcc gac ggc acg gag ctg 1836
Arg Ser Ile His Ala Gly Gln Val Cys Val Ala Asp Gly Thr Glu Leu
500 505 510
gcc gcc gcg aag ctc acc gcc gtg ctc acc aac gac ccg ggc atg ggc 1884
Ala Ala Ala Lys Leu Thr Ala Val Leu Thr Asn Asp Pro Gly Met Gly
515 520 525
gtc atc cgc cac gtc gac gcc ggc tac gac cgt gcc cac gag gtc gcc 1932
Val Ile Arg His Val Asp Ala Gly Tyr Asp Arg Ala His Glu Val Ala
530 535 540
gtc gag cgc ggg gtc cgt gtc ccc atg ccg ttc gtc agc cgc gag agg 1980
Val Glu Arg Gly Val Arg Val Pro Met Pro Phe Val Ser Arg Glu Arg
545 550 555 560
tag ccatgagcac gctgttccac ggaatctccg agctgaggac cgtctccggg 2033
ctgggcacgc tcagcgacgc cgcgctcatc gtcgaccggg gccgcatcgc ctggatcggc 2093
ccgcgcgccg gggcccccgc cgccgacgcg gcggttgatc tgggcggccg ggccgtgcta 2153
cccggctggg tggactccca cacccacatg atcttcgcgg gggaccgcgc cgccgagttc 2213
gaggcccgca tgaacgggca ggactaccag gcgggcggca tcgcggtgac catgtccgcc 2273
acccgcgagg 2283
<210> 190
<211> 560
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 190
Met Thr Glu Ser Pro Arg Thr Ile Arg Ala Pro His Gly Thr Glu Leu
1 5 10 15
Ser Ala Lys Ser Trp Gln Thr Glu Ala Pro Leu Arg Met Leu Met Asn
20 25 30
Asn Leu Asp Pro Glu Val Ala Glu Arg Pro Glu Asp Leu Val Val Tyr
35 40 45
Gly Gly Thr Gly Arg Ala Ala Arg Ser Trp Glu Ala Tyr Asp Ala Ile
50 55 60
Ile Glu Thr Leu Arg Asp Leu Glu Glu Asp Glu Thr Leu Leu Val Gln
65 70 75 80
Ser Gly Lys Pro Val Gly Val Phe Arg Thr Asn Val Trp Ala Pro Arg
85 90 95
Val Leu Ile Ala Asn Ser Asn Leu Val Gly Asp Trp Ala Asn Trp Pro
100 105 110
Glu Phe Arg Arg Leu Glu Ala Glu Gly Leu Met Met Tyr Gly Gln Met
115 120 125
Thr Ala Gly Ser Trp Ile Tyr Ile Ala Thr Gln Gly Ile Leu Gln Gly
130 135 140
Thr Tyr Glu Thr Phe Ala Ala Val Ala Arg Lys Arg Phe Gly Gly Thr
145 150 155 160
Leu Ala Gly Thr Leu Thr Leu Thr Gly Gly Cys Gly Gly Met Gly Gly
165 170 175
Ala Gln Pro Leu Ser Val Thr Leu Asn Gly Gly Val Cys Leu Ile Val
180 185 190
Asp Val Asp Glu Ser Arg Leu Arg Arg Arg Gln Ala Lys Arg Tyr Leu
195 200 205
Asp Glu Val Ala Thr Asp Leu Asp Asp Ala Val Ala Arg Val Ser Ala
210 215 220
Ala Lys Glu Glu Lys Arg Ala Leu Ser Val Gly Leu Val Gly Asn Ala
225 230 235 240
Ala Glu Val Phe Pro Glu Leu Leu Arg Arg His Arg Ala Gly Glu Ile
245 250 255
Thr Val Asp Val Val Thr Asp Gln Thr Ser Ala His Asp Pro Leu Ser
260 265 270
Tyr Leu Pro Thr Glu Ile Thr Val Glu Asp Trp Gln Arg Glu Ala Ala
275 280 285
Glu Asp Pro Ile Thr Phe Thr Lys Lys Ala Arg Glu Ser Met Ala Val
290 295 300
Gln Val Gln Ala Met Val Glu Phe Gln Asp Glu Gly Ala Glu Val Phe
305 310 315 320
Asp Tyr Gly Asn Ser Ile Arg Asp Glu Ala Arg His Ala Gly Tyr Ser
325 330 335
Arg Ala Phe Glu Phe Pro Gly Phe Val Pro Ala Tyr Ile Arg Pro Leu
340 345 350
Phe Cys Glu Gly Leu Gly Pro Phe Arg Trp Val Ala Leu Ser Gly Asp
355 360 365
Pro Glu Asp Ile Arg Val Thr Asp Glu Ala Val Lys Glu Leu Phe Pro
370 375 380
Asp Asn Glu His Leu His Arg Trp Ile Asp Ala Ala Gly Glu Tyr Val
385 390 395 400
Glu Phe Glu Gly Leu Pro Ala Arg Ile Cys Trp Leu Gly Tyr Gly Glu
405 410 415
Arg His Lys Ala Gly Leu Leu Phe Asn Arg Leu Val Ala Glu Gly Lys
420 425 430
Val Lys Ala Pro Ile Val Ile Gly Arg Asp His Leu Asp Ser Gly Ser
435 440 445
Val Ala Ser Pro Tyr Arg Glu Thr Glu Ser Met Leu Asp Gly Ser Asp
450 455 460
Ala Ile Ala Asp Trp Pro Leu Leu Asn Ala Leu Thr Ala Thr Ser Ser
465 470 475 480
Gly Ala Thr Trp Val Ser Leu His His Gly Gly Gly Val Gly Ile Gly
485 490 495
Arg Ser Ile His Ala Gly Gln Val Cys Val Ala Asp Gly Thr Glu Leu
500 505 510
Ala Ala Ala Lys Leu Thr Ala Val Leu Thr Asn Asp Pro Gly Met Gly
515 520 525
Val Ile Arg His Val Asp Ala Gly Tyr Asp Arg Ala His Glu Val Ala
530 535 540
Val Glu Arg Gly Val Arg Val Pro Met Pro Phe Val Ser Arg Glu Arg
545 550 555 560
<210> 191
<211> 1809
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1509)
<223> ген hutI (имидазолонпропионазы)
<400> 191
ctccgacgcc atcgccgact ggccgctgct caacgccctc accgccacct cctccggcgc 60
gacctgggtc tccctgcacc acggaggcgg cgtgggcatc ggacggtcca tccacgcggg 120
ccaggtgtgc gtcgccgacg gcacggagct ggccgccgcg aagctcaccg ccgtgctcac 180
caacgacccg ggcatgggcg tcatccgcca cgtcgacgcc ggctacgacc gtgcccacga 240
ggtcgccgtc gagcgcgggg tccgtgtccc catgccgttc gtcagccgcg agaggtagcc 300
atg agc acg ctg ttc cac gga atc tcc gag ctg agg acc gtc tcc ggg 348
Met Ser Thr Leu Phe His Gly Ile Ser Glu Leu Arg Thr Val Ser Gly
1 5 10 15
ctg ggc acg ctc agc gac gcc gcg ctc atc gtc gac cgg ggc cgc atc 396
Leu Gly Thr Leu Ser Asp Ala Ala Leu Ile Val Asp Arg Gly Arg Ile
20 25 30
gcc tgg atc ggc ccg cgc gcc ggg gcc ccc gcc gcc gac gcg gcg gtt 444
Ala Trp Ile Gly Pro Arg Ala Gly Ala Pro Ala Ala Asp Ala Ala Val
35 40 45
gat ctg ggc ggc cgg gcc gtg cta ccc ggc tgg gtg gac tcc cac acc 492
Asp Leu Gly Gly Arg Ala Val Leu Pro Gly Trp Val Asp Ser His Thr
50 55 60
cac atg atc ttc gcg ggg gac cgc gcc gcc gag ttc gag gcc cgc atg 540
His Met Ile Phe Ala Gly Asp Arg Ala Ala Glu Phe Glu Ala Arg Met
65 70 75 80
aac ggg cag gac tac cag gcg ggc ggc atc gcg gtg acc atg tcc gcc 588
Asn Gly Gln Asp Tyr Gln Ala Gly Gly Ile Ala Val Thr Met Ser Ala
85 90 95
acc cgc gag gcc ggc gcc gtc cgc ctc gag cag ctg ctg ctg cgc cgc 636
Thr Arg Glu Ala Gly Ala Val Arg Leu Glu Gln Leu Leu Leu Arg Arg
100 105 110
ctg gcc gat gcg cgc gcc ggg ggc acg acc acg gtg gag acg aag acc 684
Leu Ala Asp Ala Arg Ala Gly Gly Thr Thr Thr Val Glu Thr Lys Thr
115 120 125
ggc tac ggg ctc gac gtg gac tcc gag gcc ctg gcg gcc gac atc gcc 732
Gly Tyr Gly Leu Asp Val Asp Ser Glu Ala Leu Ala Ala Asp Ile Ala
130 135 140
gcc cgg cac gtc gat gac gtc acc ttc ctc ggc gcg cat ctc gtg ccg 780
Ala Arg His Val Asp Asp Val Thr Phe Leu Gly Ala His Leu Val Pro
145 150 155 160
ccg ggg gag gac ccg gac gag tac ctc gct ctc gtg ctc ggc gac atg 828
Pro Gly Glu Asp Pro Asp Glu Tyr Leu Ala Leu Val Leu Gly Asp Met
165 170 175
ctc gac gcc gtc gcg gcc aac gtc agc tgg atc gac gtg ttc tgc gaa 876
Leu Asp Ala Val Ala Ala Asn Val Ser Trp Ile Asp Val Phe Cys Glu
180 185 190
cgc ggg gcc ttc gat gag gcg gcc gcc cgc cgc gtc ctc gag gcc ggg 924
Arg Gly Ala Phe Asp Glu Ala Ala Ala Arg Arg Val Leu Glu Ala Gly
195 200 205
cgg gcg cgg ggt ctg ggg ctg cgc gtg cac ggc aac cag ctc ggg cac 972
Arg Ala Arg Gly Leu Gly Leu Arg Val His Gly Asn Gln Leu Gly His
210 215 220
ggt ccc gga gtg cag ctg gcg gtg gag tac ggc gcc gcc agc gtg gac 1020
Gly Pro Gly Val Gln Leu Ala Val Glu Tyr Gly Ala Ala Ser Val Asp
225 230 235 240
cac gtc aac tac gtg agt gac ggt gac gtg gag gca ctc gcc tcc tcg 1068
His Val Asn Tyr Val Ser Asp Gly Asp Val Glu Ala Leu Ala Ser Ser
245 250 255
gag acg gtg gcc acg gtc ctc ccc gcc tgc gac ctg tcc acg agg cag 1116
Glu Thr Val Ala Thr Val Leu Pro Ala Cys Asp Leu Ser Thr Arg Gln
260 265 270
ccg ctg gcg ccg gcc cgg agg ctt ctc gac gcc ggg gtc cac ctc gcc 1164
Pro Leu Ala Pro Ala Arg Arg Leu Leu Asp Ala Gly Val His Leu Ala
275 280 285
ctc gcc tcc aac ctc aac ccg ggc acg tcg tac acc tcg tcg atg gcc 1212
Leu Ala Ser Asn Leu Asn Pro Gly Thr Ser Tyr Thr Ser Ser Met Ala
290 295 300
ttc tgc gtc acc acc gcg gtg ctg cag cag cac ctc acc ctc gac gag 1260
Phe Cys Val Thr Thr Ala Val Leu Gln Gln His Leu Thr Leu Asp Glu
305 310 315 320
gcc atc cac gcg gcc acc gcc ggt ggg gcg cgg gcg ctg cgg cgt cac 1308
Ala Ile His Ala Ala Thr Ala Gly Gly Ala Arg Ala Leu Arg Arg His
325 330 335
gac atc ggc ggc ggc gtc gac ccc cag ggc cga ccc gcc cgc ggc agc 1356
Asp Ile Gly Gly Gly Val Asp Pro Gln Gly Arg Pro Ala Arg Gly Ser
340 345 350
ctc gtg gtc ggg gcc gcg gca gac ctg cac gtg ctg gac acc ccc cac 1404
Leu Val Val Gly Ala Ala Ala Asp Leu His Val Leu Asp Thr Pro His
355 360 365
gcc atc gat ctg gcc tac cgg ccg ggg atg ccg ctc acg tgg cgg acc 1452
Ala Ile Asp Leu Ala Tyr Arg Pro Gly Met Pro Leu Thr Trp Arg Thr
370 375 380
tac atc cag ggg gtg cag gtc cac ccg gaa ccg cag gat cag tcc gac 1500
Tyr Ile Gln Gly Val Gln Val His Pro Glu Pro Gln Asp Gln Ser Asp
385 390 395 400
ggc cga tga cgcacgatct cctccacgat ccgggccgcg acccgcgcgg 1549
Gly Arg
tccggccgtc gacgtcgaga agcgggttga gttcgacgac atcgaccaga cgcagcttcc 1609
cgctgcgcgc cacggctctg acgagcgtca ggatccgtgc cagatcgacg cccaacgccg 1669
ccggcgcgct cacgccgggg gcgaccgccg cgggcaggac gtcaaggtcg atgctcaggt 1729
ggacgtccgg ggcgtcctcc aggagctttc cgacgagcgc ctcgcacccg gccgacgtca 1789
tctccgccag ctcgacgtcg 1809
<210> 192
<211> 402
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 192
Met Ser Thr Leu Phe His Gly Ile Ser Glu Leu Arg Thr Val Ser Gly
1 5 10 15
Leu Gly Thr Leu Ser Asp Ala Ala Leu Ile Val Asp Arg Gly Arg Ile
20 25 30
Ala Trp Ile Gly Pro Arg Ala Gly Ala Pro Ala Ala Asp Ala Ala Val
35 40 45
Asp Leu Gly Gly Arg Ala Val Leu Pro Gly Trp Val Asp Ser His Thr
50 55 60
His Met Ile Phe Ala Gly Asp Arg Ala Ala Glu Phe Glu Ala Arg Met
65 70 75 80
Asn Gly Gln Asp Tyr Gln Ala Gly Gly Ile Ala Val Thr Met Ser Ala
85 90 95
Thr Arg Glu Ala Gly Ala Val Arg Leu Glu Gln Leu Leu Leu Arg Arg
100 105 110
Leu Ala Asp Ala Arg Ala Gly Gly Thr Thr Thr Val Glu Thr Lys Thr
115 120 125
Gly Tyr Gly Leu Asp Val Asp Ser Glu Ala Leu Ala Ala Asp Ile Ala
130 135 140
Ala Arg His Val Asp Asp Val Thr Phe Leu Gly Ala His Leu Val Pro
145 150 155 160
Pro Gly Glu Asp Pro Asp Glu Tyr Leu Ala Leu Val Leu Gly Asp Met
165 170 175
Leu Asp Ala Val Ala Ala Asn Val Ser Trp Ile Asp Val Phe Cys Glu
180 185 190
Arg Gly Ala Phe Asp Glu Ala Ala Ala Arg Arg Val Leu Glu Ala Gly
195 200 205
Arg Ala Arg Gly Leu Gly Leu Arg Val His Gly Asn Gln Leu Gly His
210 215 220
Gly Pro Gly Val Gln Leu Ala Val Glu Tyr Gly Ala Ala Ser Val Asp
225 230 235 240
His Val Asn Tyr Val Ser Asp Gly Asp Val Glu Ala Leu Ala Ser Ser
245 250 255
Glu Thr Val Ala Thr Val Leu Pro Ala Cys Asp Leu Ser Thr Arg Gln
260 265 270
Pro Leu Ala Pro Ala Arg Arg Leu Leu Asp Ala Gly Val His Leu Ala
275 280 285
Leu Ala Ser Asn Leu Asn Pro Gly Thr Ser Tyr Thr Ser Ser Met Ala
290 295 300
Phe Cys Val Thr Thr Ala Val Leu Gln Gln His Leu Thr Leu Asp Glu
305 310 315 320
Ala Ile His Ala Ala Thr Ala Gly Gly Ala Arg Ala Leu Arg Arg His
325 330 335
Asp Ile Gly Gly Gly Val Asp Pro Gln Gly Arg Pro Ala Arg Gly Ser
340 345 350
Leu Val Val Gly Ala Ala Ala Asp Leu His Val Leu Asp Thr Pro His
355 360 365
Ala Ile Asp Leu Ala Tyr Arg Pro Gly Met Pro Leu Thr Trp Arg Thr
370 375 380
Tyr Ile Gln Gly Val Gln Val His Pro Glu Pro Gln Asp Gln Ser Asp
385 390 395 400
Gly Arg
<210> 193
<211> 2151
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1851)
<223> ген hutH (гистидинаммиаклиаза)
<400> 193
ccccggcgtc gtcggtaccg gcccggaatg ccatccgtgc agctcagagc cctgacgggc 60
atctcgtatg cgagatagat tccgcgcaca ccggaaagtc tcgtaagtga gacagccgtg 120
cccttcacgg gctgtcgccg gggcgtgacg gctgacacac tgggaggaac tgcgtccggc 180
tgtcgcgcag gtcgaaccgt ccacgagtca ccgccccgga ggacaggtac cacctgtgcc 240
tgccccgggg ccggtgcccc ggagaaaccg ggacccacca ggaagcaggg gaagccccac 300
atg cag acc atc acc tcc cac atc cgc gtc ggc gtc gga gcg ctc agc 348
Met Gln Thr Ile Thr Ser His Ile Arg Val Gly Val Gly Ala Leu Ser
1 5 10 15
atc gac gag gtc gtg gct gtc gcc cgg cac gac gcc cac gtc gag atc 396
Ile Asp Glu Val Val Ala Val Ala Arg His Asp Ala His Val Glu Ile
20 25 30
acc ccg gag gcg ctc gag gcc gtc gcc gcc acc cgt gcc cgc atc gag 444
Thr Pro Glu Ala Leu Glu Ala Val Ala Ala Thr Arg Ala Arg Ile Glu
35 40 45
gaa ctg gcc gac cac ccg acc ccg gtg tac ggc gtg tcc acc ggt ttc 492
Glu Leu Ala Asp His Pro Thr Pro Val Tyr Gly Val Ser Thr Gly Phe
50 55 60
ggc gcc ctg gcc cgc cgc tac atc ccc cag gag atg cgc ctc cag ctg 540
Gly Ala Leu Ala Arg Arg Tyr Ile Pro Gln Glu Met Arg Leu Gln Leu
65 70 75 80
cag cgc agc ctc gtc cgc tcc cat gcc gcc ggc tcc ggc ccc gag gtg 588
Gln Arg Ser Leu Val Arg Ser His Ala Ala Gly Ser Gly Pro Glu Val
85 90 95
gag cgc gag gtc gtc cgg gcg ctc atg ctg ctg cgc ctg tcc aca ctg 636
Glu Arg Glu Val Val Arg Ala Leu Met Leu Leu Arg Leu Ser Thr Leu
100 105 110
tgc acc ggc cgt acc ggc gtg cgg ccg gtc gtc gcg gag acc tac gcc 684
Cys Thr Gly Arg Thr Gly Val Arg Pro Val Val Ala Glu Thr Tyr Ala
115 120 125
gcg gtg ctc aac gcc ggc atc acc ccc gtc gtc cac gag tac ggc tcg 732
Ala Val Leu Asn Ala Gly Ile Thr Pro Val Val His Glu Tyr Gly Ser
130 135 140
ctc ggc tgc tcc ggc gac ctc tcc ccg ctg gcg cac tgc gcc ctg gcg 780
Leu Gly Cys Ser Gly Asp Leu Ser Pro Leu Ala His Cys Ala Leu Ala
145 150 155 160
ctg ctg ggg gag ggc gag gtc acc acc gct gac ggc cgc cgc ctg ccc 828
Leu Leu Gly Glu Gly Glu Val Thr Thr Ala Asp Gly Arg Arg Leu Pro
165 170 175
gcc gcc gtc gcc ctc gcc gag cac ggc atc gag ccg ctg gtc ctg cgg 876
Ala Ala Val Ala Leu Ala Glu His Gly Ile Glu Pro Leu Val Leu Arg
180 185 190
gag aag gag ggg ctg gca ctc atc aac ggc acc gac ggc atg ctg ggc 924
Glu Lys Glu Gly Leu Ala Leu Ile Asn Gly Thr Asp Gly Met Leu Gly
195 200 205
atg ctg tgc ctg gcc atc gct gac ctc gag tgc ctg gcc cgc agc gcc 972
Met Leu Cys Leu Ala Ile Ala Asp Leu Glu Cys Leu Ala Arg Ser Ala
210 215 220
gac atc gcc gcc gcg atg acc gtc gag gcc ctg cgg ggc acc ctc acc 1020
Asp Ile Ala Ala Ala Met Thr Val Glu Ala Leu Arg Gly Thr Leu Thr
225 230 235 240
gtc ttc gcc gcg gac ctg cag gag ctg cgc ccg cac ccg ggt cag gcg 1068
Val Phe Ala Ala Asp Leu Gln Glu Leu Arg Pro His Pro Gly Gln Ala
245 250 255
gcc tcc gcg gcc aac atc cgc gcc gtg gcc gag ggc tcc gcc ata ctg 1116
Ala Ser Ala Ala Asn Ile Arg Ala Val Ala Glu Gly Ser Ala Ile Leu
260 265 270
cag tcc gcc acc gag gaa ttc gcc ctg acc cac gtg cag gac gcc tac 1164
Gln Ser Ala Thr Glu Glu Phe Ala Leu Thr His Val Gln Asp Ala Tyr
275 280 285
tcg gtg cgg tgc gcc ccg cag gtc gcc ggt ggt ttc cgc gac acc ctg 1212
Ser Val Arg Cys Ala Pro Gln Val Ala Gly Gly Phe Arg Asp Thr Leu
290 295 300
gcc cac gcc cgc ctg gtc gcc gac cgg gag ctc gcc tcc gcc gtg gac 1260
Ala His Ala Arg Leu Val Ala Asp Arg Glu Leu Ala Ser Ala Val Asp
305 310 315 320
aac ccc gtg gtg gct ctc gac ggc cgc gtg acc tcc aac ggc aac ttc 1308
Asn Pro Val Val Ala Leu Asp Gly Arg Val Thr Ser Asn Gly Asn Phe
325 330 335
cac ggc gcg ccc gtc ggc tac gcg ctc gac ttc ctc gcc atc gtc acc 1356
His Gly Ala Pro Val Gly Tyr Ala Leu Asp Phe Leu Ala Ile Val Thr
340 345 350
gcc gac ctg gcg agc atc tcc gag cgc cgc acc gac cgg ctt ctc gac 1404
Ala Asp Leu Ala Ser Ile Ser Glu Arg Arg Thr Asp Arg Leu Leu Asp
355 360 365
gtc gcc cgc agc cgc gga ctc aac gcc ttc ctc gcc gac gat ccg ggc 1452
Val Ala Arg Ser Arg Gly Leu Asn Ala Phe Leu Ala Asp Asp Pro Gly
370 375 380
gtg gac tcc ggc cac atg atc gcc cag tac acg cag gcc ggg atc gtc 1500
Val Asp Ser Gly His Met Ile Ala Gln Tyr Thr Gln Ala Gly Ile Val
385 390 395 400
tcc gag ctc aag cgg ctg gcc gcc ccg gcc agc gtg gac tcc atc ccc 1548
Ser Glu Leu Lys Arg Leu Ala Ala Pro Ala Ser Val Asp Ser Ile Pro
405 410 415
tcc tcc gcc atg cag gag gac cac gtg tcc atg ggc tgg tcg gcc ggg 1596
Ser Ser Ala Met Gln Glu Asp His Val Ser Met Gly Trp Ser Ala Gly
420 425 430
cgc aag ctg cgc cgt tcc gtc gac ggg ctg ggg cgg gtg ctc ggc gtc 1644
Arg Lys Leu Arg Arg Ser Val Asp Gly Leu Gly Arg Val Leu Gly Val
435 440 445
gag gtg ctc acc gca gcc cgg gcc atc gac atg cgc gag cag gag ccc 1692
Glu Val Leu Thr Ala Ala Arg Ala Ile Asp Met Arg Glu Gln Glu Pro
450 455 460
gcg ccg ggg acc cgc gcc gca ctg gac gcc ctg cgc ggc cgt gtg gag 1740
Ala Pro Gly Thr Arg Ala Ala Leu Asp Ala Leu Arg Gly Arg Val Glu
465 470 475 480
ggg ccc ggc ccc gac cgc tac ctg tcc ccg gag atc gag gag tcg gtg 1788
Gly Pro Gly Pro Asp Arg Tyr Leu Ser Pro Glu Ile Glu Glu Ser Val
485 490 495
cag ctg ctc acc tcc ggg gcg ctg ctg gag gcg gtg gag ggg gtc gtc 1836
Gln Leu Leu Thr Ser Gly Ala Leu Leu Glu Ala Val Glu Gly Val Val
500 505 510
gga aag ctg gca tga gcgggcgaat ctcagatatg agactaaagg ggcctccggg 1891
Gly Lys Leu Ala
515
ccctcgatcc cgactgtgac ctgtcttacc gtcagaagtg tcaccgacac cggccacacc 1951
agcccggtgc tccacacccg acgatgacag ccccgggcca cccggatcgc gggtgccccg 2011
gggccccggc ctgatcggcc cccaccgtag aaggagaccc accccatgac cgagtccccg 2071
cgcaccatcc gtgccccgca cggcaccgag ctcagcgcca agagctggca aaccgaggca 2131
cccctgcgca tgctcatgaa 2151
<210> 194
<211> 516
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 194
Met Gln Thr Ile Thr Ser His Ile Arg Val Gly Val Gly Ala Leu Ser
1 5 10 15
Ile Asp Glu Val Val Ala Val Ala Arg His Asp Ala His Val Glu Ile
20 25 30
Thr Pro Glu Ala Leu Glu Ala Val Ala Ala Thr Arg Ala Arg Ile Glu
35 40 45
Glu Leu Ala Asp His Pro Thr Pro Val Tyr Gly Val Ser Thr Gly Phe
50 55 60
Gly Ala Leu Ala Arg Arg Tyr Ile Pro Gln Glu Met Arg Leu Gln Leu
65 70 75 80
Gln Arg Ser Leu Val Arg Ser His Ala Ala Gly Ser Gly Pro Glu Val
85 90 95
Glu Arg Glu Val Val Arg Ala Leu Met Leu Leu Arg Leu Ser Thr Leu
100 105 110
Cys Thr Gly Arg Thr Gly Val Arg Pro Val Val Ala Glu Thr Tyr Ala
115 120 125
Ala Val Leu Asn Ala Gly Ile Thr Pro Val Val His Glu Tyr Gly Ser
130 135 140
Leu Gly Cys Ser Gly Asp Leu Ser Pro Leu Ala His Cys Ala Leu Ala
145 150 155 160
Leu Leu Gly Glu Gly Glu Val Thr Thr Ala Asp Gly Arg Arg Leu Pro
165 170 175
Ala Ala Val Ala Leu Ala Glu His Gly Ile Glu Pro Leu Val Leu Arg
180 185 190
Glu Lys Glu Gly Leu Ala Leu Ile Asn Gly Thr Asp Gly Met Leu Gly
195 200 205
Met Leu Cys Leu Ala Ile Ala Asp Leu Glu Cys Leu Ala Arg Ser Ala
210 215 220
Asp Ile Ala Ala Ala Met Thr Val Glu Ala Leu Arg Gly Thr Leu Thr
225 230 235 240
Val Phe Ala Ala Asp Leu Gln Glu Leu Arg Pro His Pro Gly Gln Ala
245 250 255
Ala Ser Ala Ala Asn Ile Arg Ala Val Ala Glu Gly Ser Ala Ile Leu
260 265 270
Gln Ser Ala Thr Glu Glu Phe Ala Leu Thr His Val Gln Asp Ala Tyr
275 280 285
Ser Val Arg Cys Ala Pro Gln Val Ala Gly Gly Phe Arg Asp Thr Leu
290 295 300
Ala His Ala Arg Leu Val Ala Asp Arg Glu Leu Ala Ser Ala Val Asp
305 310 315 320
Asn Pro Val Val Ala Leu Asp Gly Arg Val Thr Ser Asn Gly Asn Phe
325 330 335
His Gly Ala Pro Val Gly Tyr Ala Leu Asp Phe Leu Ala Ile Val Thr
340 345 350
Ala Asp Leu Ala Ser Ile Ser Glu Arg Arg Thr Asp Arg Leu Leu Asp
355 360 365
Val Ala Arg Ser Arg Gly Leu Asn Ala Phe Leu Ala Asp Asp Pro Gly
370 375 380
Val Asp Ser Gly His Met Ile Ala Gln Tyr Thr Gln Ala Gly Ile Val
385 390 395 400
Ser Glu Leu Lys Arg Leu Ala Ala Pro Ala Ser Val Asp Ser Ile Pro
405 410 415
Ser Ser Ala Met Gln Glu Asp His Val Ser Met Gly Trp Ser Ala Gly
420 425 430
Arg Lys Leu Arg Arg Ser Val Asp Gly Leu Gly Arg Val Leu Gly Val
435 440 445
Glu Val Leu Thr Ala Ala Arg Ala Ile Asp Met Arg Glu Gln Glu Pro
450 455 460
Ala Pro Gly Thr Arg Ala Ala Leu Asp Ala Leu Arg Gly Arg Val Glu
465 470 475 480
Gly Pro Gly Pro Asp Arg Tyr Leu Ser Pro Glu Ile Glu Glu Ser Val
485 490 495
Gln Leu Leu Thr Ser Gly Ala Leu Leu Glu Ala Val Glu Gly Val Val
500 505 510
Gly Lys Leu Ala
515
<210> 195
<211> 1509
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1209)
<223> ген hutG (формидидоилглутамазы)
<400> 195
cgagtccgta ggtgcgggtc tcggggatgc gcacgacgta gccctccgac tccatgacgg 60
cgagcaggtg gtacgtcgac gaccggggga ggtcgagttc cgtcatgatg cgtgccgccg 120
agacgggcac gtcgatggat gagagcagcg agaggatgcg caacgtgtgt cgcgcggcgg 180
gcacctgtcc catgtcactc cttacctgtg tgtggtctgt gggtcggcgg tctcgtatgc 240
gggaccacgc tgcgtcgcag ccgggtgtgg cgggaggtcg gtggcgttat cctacggcgc 300
atg acc acc cat gaa tgg aca gga cgc gac gac ggg ccc ggg gcc ggg 348
Met Thr Thr His Glu Trp Thr Gly Arg Asp Asp Gly Pro Gly Ala Gly
1 5 10 15
cac gcc cgt tgg cac agc gtc atc acc acc acg acc gac cgg ccg ggg 396
His Ala Arg Trp His Ser Val Ile Thr Thr Thr Thr Asp Arg Pro Gly
20 25 30
gtc gcc ctc gtc ggg ttc gcc tcc gac gag ggg gtc cgc cgc aac ggt 444
Val Ala Leu Val Gly Phe Ala Ser Asp Glu Gly Val Arg Arg Asn Gly
35 40 45
ggc cgc ccc ggc gcc gcc gcc ggc ccc acg gag atc cgc cgt gcc ctg 492
Gly Arg Pro Gly Ala Ala Ala Gly Pro Thr Glu Ile Arg Arg Ala Leu
50 55 60
gcc tcc ctg gcc gtg cac acc gac gtc ccg ctt ctc gac gcc ggc acc 540
Ala Ser Leu Ala Val His Thr Asp Val Pro Leu Leu Asp Ala Gly Thr
65 70 75 80
gtc acc gtc tcc ggt acc gac ctg gag ggc ggg cac gac gca ctg tcc 588
Val Thr Val Ser Gly Thr Asp Leu Glu Gly Gly His Asp Ala Leu Ser
85 90 95
gcg cag gtg gag gag ctg acc cgc gcg ggg cac acc gtc atc gtg ctg 636
Ala Gln Val Glu Glu Leu Thr Arg Ala Gly His Thr Val Ile Val Leu
100 105 110
ggc ggt ggg cat gag acg gcg ttc ggg tcg cac cgc ggg gtg cgc cgg 684
Gly Gly Gly His Glu Thr Ala Phe Gly Ser His Arg Gly Val Arg Arg
115 120 125
gcg ctg ggg cac agt ccg gcg atc atc aac ctc gac gcc cac ttc gac 732
Ala Leu Gly His Ser Pro Ala Ile Ile Asn Leu Asp Ala His Phe Asp
130 135 140
ctg cgg gtc gcg ccg gcc ccg tcc tcg ggt acc ccg ttc cgg cag atc 780
Leu Arg Val Ala Pro Ala Pro Ser Ser Gly Thr Pro Phe Arg Gln Ile
145 150 155 160
gcg gac ctg gcc ggg gag gag ttc cgt tat gcg gtg ctg ggc atc tcg 828
Ala Asp Leu Ala Gly Glu Glu Phe Arg Tyr Ala Val Leu Gly Ile Ser
165 170 175
gtg ccc aac aac acc cgg gcg ctg ttc gac acc gcc cgg gaa ctg ggg 876
Val Pro Asn Asn Thr Arg Ala Leu Phe Asp Thr Ala Arg Glu Leu Gly
180 185 190
gtc cgg gtg acc acc gac gtc gag ctg gcg gag atg acg tcg gcc ggg 924
Val Arg Val Thr Thr Asp Val Glu Leu Ala Glu Met Thr Ser Ala Gly
195 200 205
tgc gag gcg ctc gtc gga aag ctc ctg gag gac gcc ccg gac gtc cac 972
Cys Glu Ala Leu Val Gly Lys Leu Leu Glu Asp Ala Pro Asp Val His
210 215 220
ctg agc atc gac ctt gac gtc ctg ccc gcg gcg gtc gcc ccc ggc gtg 1020
Leu Ser Ile Asp Leu Asp Val Leu Pro Ala Ala Val Ala Pro Gly Val
225 230 235 240
agc gcg ccg gcg gcg ttg ggc gtc gat ctg gca cgg atc ctg acg ctc 1068
Ser Ala Pro Ala Ala Leu Gly Val Asp Leu Ala Arg Ile Leu Thr Leu
245 250 255
gtc aga gcc gtg gcg cgc agc ggg aag ctg cgt ctg gtc gat gtc gtc 1116
Val Arg Ala Val Ala Arg Ser Gly Lys Leu Arg Leu Val Asp Val Val
260 265 270
gaa ctc aac ccg ctt ctc gac gtc gac ggc cgg acc gcg cgg gtc gcg 1164
Glu Leu Asn Pro Leu Leu Asp Val Asp Gly Arg Thr Ala Arg Val Ala
275 280 285
gcc cgg atc gtg gag gag atc gtg cgt cat cgg ccg tcg gac tga 1209
Ala Arg Ile Val Glu Glu Ile Val Arg His Arg Pro Ser Asp
290 295 300
tcctgcggtt ccgggtggac ctgcaccccc tggatgtagg tccgccacgt gagcggcatc 1269
cccggccggt aggccagatc gatggcgtgg ggggtgtcca gcacgtgcag gtctgccgcg 1329
gccccgacca cgaggctgcc gcgggcgggt cggccctggg ggtcgacgcc gccgccgatg 1389
tcgtgacgcc gcagcgcccg cgccccaccg gcggtggccg cgtggatggc ctcgtcgagg 1449
gtgaggtgct gctgcagcac cgcggtggtg acgcagaagg ccatcgacga ggtgtacgac 1509
<210> 196
<211> 302
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 196
Met Thr Thr His Glu Trp Thr Gly Arg Asp Asp Gly Pro Gly Ala Gly
1 5 10 15
His Ala Arg Trp His Ser Val Ile Thr Thr Thr Thr Asp Arg Pro Gly
20 25 30
Val Ala Leu Val Gly Phe Ala Ser Asp Glu Gly Val Arg Arg Asn Gly
35 40 45
Gly Arg Pro Gly Ala Ala Ala Gly Pro Thr Glu Ile Arg Arg Ala Leu
50 55 60
Ala Ser Leu Ala Val His Thr Asp Val Pro Leu Leu Asp Ala Gly Thr
65 70 75 80
Val Thr Val Ser Gly Thr Asp Leu Glu Gly Gly His Asp Ala Leu Ser
85 90 95
Ala Gln Val Glu Glu Leu Thr Arg Ala Gly His Thr Val Ile Val Leu
100 105 110
Gly Gly Gly His Glu Thr Ala Phe Gly Ser His Arg Gly Val Arg Arg
115 120 125
Ala Leu Gly His Ser Pro Ala Ile Ile Asn Leu Asp Ala His Phe Asp
130 135 140
Leu Arg Val Ala Pro Ala Pro Ser Ser Gly Thr Pro Phe Arg Gln Ile
145 150 155 160
Ala Asp Leu Ala Gly Glu Glu Phe Arg Tyr Ala Val Leu Gly Ile Ser
165 170 175
Val Pro Asn Asn Thr Arg Ala Leu Phe Asp Thr Ala Arg Glu Leu Gly
180 185 190
Val Arg Val Thr Thr Asp Val Glu Leu Ala Glu Met Thr Ser Ala Gly
195 200 205
Cys Glu Ala Leu Val Gly Lys Leu Leu Glu Asp Ala Pro Asp Val His
210 215 220
Leu Ser Ile Asp Leu Asp Val Leu Pro Ala Ala Val Ala Pro Gly Val
225 230 235 240
Ser Ala Pro Ala Ala Leu Gly Val Asp Leu Ala Arg Ile Leu Thr Leu
245 250 255
Val Arg Ala Val Ala Arg Ser Gly Lys Leu Arg Leu Val Asp Val Val
260 265 270
Glu Leu Asn Pro Leu Leu Asp Val Asp Gly Arg Thr Ala Arg Val Ala
275 280 285
Ala Arg Ile Val Glu Glu Ile Val Arg His Arg Pro Ser Asp
290 295 300
<210> 197
<211> 1767
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1467)
<223> ген sucC
<400> 197
gctggccggt ccggacttcc ggtacctcga tcacatcaag ccgatctacg accagcccat 60
cggtgaggcg acctggcgcg acatgtccac ctggttcacg tggcagagca ggggagaccg 120
tttcggctac ggccacctga cgtcggaact ctgcagcggt cggctcgggg agctggtgga 180
acgcttcctg gagctgtggg acgcgcccac cacggcgtag cgcctccgtc cggggcgtgt 240
ggccgcaggg tatcgccgat ccggcgtcgt gcgtgcggca acgtctatac actgcagggc 300
atg gac ctc tac gag tac cag gcc ctt gaa ctc ttc gcc gcc cac ggc 348
Met Asp Leu Tyr Glu Tyr Gln Ala Leu Glu Leu Phe Ala Ala His Gly
1 5 10 15
gtt ccg gtt ctg tcc gga acg gtg gct gag act ccg gcg gag gca cgc 396
Val Pro Val Leu Ser Gly Thr Val Ala Glu Thr Pro Ala Glu Ala Arg
20 25 30
gcg gcg gcg gag cgg ctg ggc ggg gag tgc gtc gtc aag gcg cag gtg 444
Ala Ala Ala Glu Arg Leu Gly Gly Glu Cys Val Val Lys Ala Gln Val
35 40 45
aag atc ggc ggc cgc ggc aag gcg ggc ggt gtc cgc gtc gcg gcg gcg 492
Lys Ile Gly Gly Arg Gly Lys Ala Gly Gly Val Arg Val Ala Ala Ala
50 55 60
ccc gag gcg gcc gag cag gcg gcg gag gcg atc ctg ggg atg gac atc 540
Pro Glu Ala Ala Glu Gln Ala Ala Glu Ala Ile Leu Gly Met Asp Ile
65 70 75 80
cgc ggg cac acg gtc gag cgg gtg ctc gtc gtg gag tgc gtg gag atc 588
Arg Gly His Thr Val Glu Arg Val Leu Val Val Glu Cys Val Glu Ile
85 90 95
gag gcg gag tac tac ttc tcg ctg ctc ctc gac cgc gcg cgg cgc tcc 636
Glu Ala Glu Tyr Tyr Phe Ser Leu Leu Leu Asp Arg Ala Arg Arg Ser
100 105 110
tac ctg ggg ttg ttc tcg gtg gag gga ggc gtg gac atc gag aag ctc 684
Tyr Leu Gly Leu Phe Ser Val Glu Gly Gly Val Asp Ile Glu Lys Leu
115 120 125
gcc gcg gag cgg ccg gag gcg ctg gcc cgc gtg gag gtc gac ccc ctc 732
Ala Ala Glu Arg Pro Glu Ala Leu Ala Arg Val Glu Val Asp Pro Leu
130 135 140
gag ggc ttc gac gcc cac gaa ctt gtc gac gcc ctc ccc gcc gac ctc 780
Glu Gly Phe Asp Ala His Glu Leu Val Asp Ala Leu Pro Ala Asp Leu
145 150 155 160
cgc gac aag gtc gtc ccc gtc atc cgg cgg ctc gcc gag gtc ttc cgc 828
Arg Asp Lys Val Val Pro Val Ile Arg Arg Leu Ala Glu Val Phe Arg
165 170 175
gag gag gac gcc acc ctc gtc gag gtg aac ccg ctc gtc ctc acg gtg 876
Glu Glu Asp Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Val
180 185 190
cag ggg gac atc atc gcc ctc gac gcg aag atc acc ctc gac gac aac 924
Gln Gly Asp Ile Ile Ala Leu Asp Ala Lys Ile Thr Leu Asp Asp Asn
195 200 205
gcc gcc ttc cgt cac gac aac cgc gcg gag ctc gcc gag gcc gcc gac 972
Ala Ala Phe Arg His Asp Asn Arg Ala Glu Leu Ala Glu Ala Ala Asp
210 215 220
cac ctc gac ccg atc gag cga cgc gcg agc gag cag ggg ctc aag tac 1020
His Leu Asp Pro Ile Glu Arg Arg Ala Ser Glu Gln Gly Leu Lys Tyr
225 230 235 240
gtc acg ctc gag ggt tcg gtg ggc atc atc ggc aac ggc gcc ggg ctg 1068
Val Thr Leu Glu Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu
245 250 255
gtc atg tcg acc ctc gac gtc gtg tcc ggg gcg ggg gag cgg cac ggc 1116
Val Met Ser Thr Leu Asp Val Val Ser Gly Ala Gly Glu Arg His Gly
260 265 270
ggg cag aag ccg gcg aac ttc ctc gac atc ggc ggt ggc gcc tgc ccg 1164
Gly Gln Lys Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Cys Pro
275 280 285
gag acg atg acc gcg gcc ctg gag atc gtc ctc tcg gac gag cag gtg 1212
Glu Thr Met Thr Ala Ala Leu Glu Ile Val Leu Ser Asp Glu Gln Val
290 295 300
cgc agc gtg ttc gtc aac gtc ttc gga ggc atc acg tcc tgc gac gcg 1260
Arg Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ser Cys Asp Ala
305 310 315 320
gtg gcc acc ggc atc gtg gag gcg ctc act cac ctc ggg gac acg gcc 1308
Val Ala Thr Gly Ile Val Glu Ala Leu Thr His Leu Gly Asp Thr Ala
325 330 335
cgc aag ccg atc gtc gtg cgt ctc gac ggc aac agc gtc gac gag gga 1356
Arg Lys Pro Ile Val Val Arg Leu Asp Gly Asn Ser Val Asp Glu Gly
340 345 350
cgc cgc atc ctc cgc gag tac gat cac ccc ctc gtc acc gtc gtc gac 1404
Arg Arg Ile Leu Arg Glu Tyr Asp His Pro Leu Val Thr Val Val Asp
355 360 365
gac atg gac gag gcc gcg gac cgg gtg gcg gag ctc gcc gcg gtg acc 1452
Asp Met Asp Glu Ala Ala Asp Arg Val Ala Glu Leu Ala Ala Val Thr
370 375 380
ccg agc gtc gtc tag cgaaaggcac cgacatggcg atcttcctca actccgaatc 1507
Pro Ser Val Val
385
ccgcatcatc gtccagggca tcaccgggcg tgaggggcgg gagcacaccg cccggatgct 1567
cgccgccggc gcgaacgtgg tcggcggcac caacccgaag aaggcgggcg agacggtgac 1627
ggtcggcggc cacgagctgc cggtgttcgg caccgtcgcc gacgcacgcg cggccaccga 1687
cgcgaacgtg tccgtggtgt tcgtgcctgc ccccttcgtc cgggaggcgg tctgcgaggc 1747
catcgacgcc gcgatcccgc 1767
<210> 198
<211> 388
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 198
Met Asp Leu Tyr Glu Tyr Gln Ala Leu Glu Leu Phe Ala Ala His Gly
1 5 10 15
Val Pro Val Leu Ser Gly Thr Val Ala Glu Thr Pro Ala Glu Ala Arg
20 25 30
Ala Ala Ala Glu Arg Leu Gly Gly Glu Cys Val Val Lys Ala Gln Val
35 40 45
Lys Ile Gly Gly Arg Gly Lys Ala Gly Gly Val Arg Val Ala Ala Ala
50 55 60
Pro Glu Ala Ala Glu Gln Ala Ala Glu Ala Ile Leu Gly Met Asp Ile
65 70 75 80
Arg Gly His Thr Val Glu Arg Val Leu Val Val Glu Cys Val Glu Ile
85 90 95
Glu Ala Glu Tyr Tyr Phe Ser Leu Leu Leu Asp Arg Ala Arg Arg Ser
100 105 110
Tyr Leu Gly Leu Phe Ser Val Glu Gly Gly Val Asp Ile Glu Lys Leu
115 120 125
Ala Ala Glu Arg Pro Glu Ala Leu Ala Arg Val Glu Val Asp Pro Leu
130 135 140
Glu Gly Phe Asp Ala His Glu Leu Val Asp Ala Leu Pro Ala Asp Leu
145 150 155 160
Arg Asp Lys Val Val Pro Val Ile Arg Arg Leu Ala Glu Val Phe Arg
165 170 175
Glu Glu Asp Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Val
180 185 190
Gln Gly Asp Ile Ile Ala Leu Asp Ala Lys Ile Thr Leu Asp Asp Asn
195 200 205
Ala Ala Phe Arg His Asp Asn Arg Ala Glu Leu Ala Glu Ala Ala Asp
210 215 220
His Leu Asp Pro Ile Glu Arg Arg Ala Ser Glu Gln Gly Leu Lys Tyr
225 230 235 240
Val Thr Leu Glu Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu
245 250 255
Val Met Ser Thr Leu Asp Val Val Ser Gly Ala Gly Glu Arg His Gly
260 265 270
Gly Gln Lys Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Cys Pro
275 280 285
Glu Thr Met Thr Ala Ala Leu Glu Ile Val Leu Ser Asp Glu Gln Val
290 295 300
Arg Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ser Cys Asp Ala
305 310 315 320
Val Ala Thr Gly Ile Val Glu Ala Leu Thr His Leu Gly Asp Thr Ala
325 330 335
Arg Lys Pro Ile Val Val Arg Leu Asp Gly Asn Ser Val Asp Glu Gly
340 345 350
Arg Arg Ile Leu Arg Glu Tyr Asp His Pro Leu Val Thr Val Val Asp
355 360 365
Asp Met Asp Glu Ala Ala Asp Arg Val Ala Glu Leu Ala Ala Val Thr
370 375 380
Pro Ser Val Val
385
<210> 199
<211> 1455
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1155)
<223> ген sucD
<400> 199
cctggagatc gtcctctcgg acgagcaggt gcgcagcgtg ttcgtcaacg tcttcggagg 60
catcacgtcc tgcgacgcgg tggccaccgg catcgtggag gcgctcactc acctcgggga 120
cacggcccgc aagccgatcg tcgtgcgtct cgacggcaac agcgtcgacg agggacgccg 180
catcctccgc gagtacgatc accccctcgt caccgtcgtc gacgacatgg acgaggccgc 240
ggaccgggtg gcggagctcg ccgcggtgac cccgagcgtc gtctagcgaa aggcaccgac 300
atg gcg atc ttc ctc aac tcc gaa tcc cgc atc atc gtc cag ggc atc 348
Met Ala Ile Phe Leu Asn Ser Glu Ser Arg Ile Ile Val Gln Gly Ile
1 5 10 15
acc ggg cgt gag ggg cgg gag cac acc gcc cgg atg ctc gcc gcc ggc 396
Thr Gly Arg Glu Gly Arg Glu His Thr Ala Arg Met Leu Ala Ala Gly
20 25 30
gcg aac gtg gtc ggc ggc acc aac ccg aag aag gcg ggc gag acg gtg 444
Ala Asn Val Val Gly Gly Thr Asn Pro Lys Lys Ala Gly Glu Thr Val
35 40 45
acg gtc ggc ggc cac gag ctg ccg gtg ttc ggc acc gtc gcc gac gca 492
Thr Val Gly Gly His Glu Leu Pro Val Phe Gly Thr Val Ala Asp Ala
50 55 60
cgc gcg gcc acc gac gcg aac gtg tcc gtg gtg ttc gtg cct gcc ccc 540
Arg Ala Ala Thr Asp Ala Asn Val Ser Val Val Phe Val Pro Ala Pro
65 70 75 80
ttc gtc cgg gag gcg gtc tgc gag gcc atc gac gcc gcg atc ccg ctc 588
Phe Val Arg Glu Ala Val Cys Glu Ala Ile Asp Ala Ala Ile Pro Leu
85 90 95
atc gtc gtc atc acc gag ggc gtg ccc gtc cgc gac acc gcc gag atg 636
Ile Val Val Ile Thr Glu Gly Val Pro Val Arg Asp Thr Ala Glu Met
100 105 110
ctg gcg cac gcg cgg acg gag gga ggc acg cgg atc atc ggc ccg aac 684
Leu Ala His Ala Arg Thr Glu Gly Gly Thr Arg Ile Ile Gly Pro Asn
115 120 125
tgc ccc ggc atc atc ccc gcg gat atc agc ggc ccc ggc ccc gtg gga 732
Cys Pro Gly Ile Ile Pro Ala Asp Ile Ser Gly Pro Gly Pro Val Gly
130 135 140
ctg atc tcg aag tcg ggc acg ctg acg tac cag atg atg cac gcg ctt 780
Leu Ile Ser Lys Ser Gly Thr Leu Thr Tyr Gln Met Met His Ala Leu
145 150 155 160
tcc gac gtc ggc atc tcc acc gcc ctc ggc atc ggc ggc gac ccc ctc 828
Ser Asp Val Gly Ile Ser Thr Ala Leu Gly Ile Gly Gly Asp Pro Leu
165 170 175
atc ggc acc gcc tac atc gac gcg ctc agc gcc ttc gag gag gac ccc 876
Ile Gly Thr Ala Tyr Ile Asp Ala Leu Ser Ala Phe Glu Glu Asp Pro
180 185 190
gac acc cgg gcc atc gtc atg atc ggc gag atc ggc ggc gac gcc gag 924
Asp Thr Arg Ala Ile Val Met Ile Gly Glu Ile Gly Gly Asp Ala Glu
195 200 205
gaa cgc gcc gcc gac tac atc cgg gag cac gtg acc aaa ccc gtc gtc 972
Glu Arg Ala Ala Asp Tyr Ile Arg Glu His Val Thr Lys Pro Val Val
210 215 220
ggc tac gtc gcc ggg ttc acc gcc ccg gag ggc aag acc atg ggg cac 1020
Gly Tyr Val Ala Gly Phe Thr Ala Pro Glu Gly Lys Thr Met Gly His
225 230 235 240
gca ggt gcc atc gtc acc ggt tcc gtc ggc acg gcg cag gcc aag aag 1068
Ala Gly Ala Ile Val Thr Gly Ser Val Gly Thr Ala Gln Ala Lys Lys
245 250 255
gag gcg ctc gag gcg gcc ggt gtc cgg gtg ggc acc acg ccg acg gag 1116
Glu Ala Leu Glu Ala Ala Gly Val Arg Val Gly Thr Thr Pro Thr Glu
260 265 270
acc gcg gag atc atg cgg ggg att gtc gag ggg atg tag gctgcccggg 1165
Thr Ala Glu Ile Met Arg Gly Ile Val Glu Gly Met
275 280
tcgcgtcggc ggcctatcat cggagcatgc tggagaccgg acgcacccac cccctggcag 1225
acatcattga cacggtcctt gccgacccga cctcgggctc cggctggtgc ctgtacacag 1285
gcccggggat gaccccgggg gagtacctcg tcgacgagta ccccgaggtc ggcgatgacg 1345
ataccgagac ctatccgccg gcggtgaggg aacgaggtct ggactatttc ctcagtggtc 1405
agatgtgcga ggacgtgatc ctcaatctgg accaccaggg atccccactt 1455
<210> 200
<211> 284
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 200
Met Ala Ile Phe Leu Asn Ser Glu Ser Arg Ile Ile Val Gln Gly Ile
1 5 10 15
Thr Gly Arg Glu Gly Arg Glu His Thr Ala Arg Met Leu Ala Ala Gly
20 25 30
Ala Asn Val Val Gly Gly Thr Asn Pro Lys Lys Ala Gly Glu Thr Val
35 40 45
Thr Val Gly Gly His Glu Leu Pro Val Phe Gly Thr Val Ala Asp Ala
50 55 60
Arg Ala Ala Thr Asp Ala Asn Val Ser Val Val Phe Val Pro Ala Pro
65 70 75 80
Phe Val Arg Glu Ala Val Cys Glu Ala Ile Asp Ala Ala Ile Pro Leu
85 90 95
Ile Val Val Ile Thr Glu Gly Val Pro Val Arg Asp Thr Ala Glu Met
100 105 110
Leu Ala His Ala Arg Thr Glu Gly Gly Thr Arg Ile Ile Gly Pro Asn
115 120 125
Cys Pro Gly Ile Ile Pro Ala Asp Ile Ser Gly Pro Gly Pro Val Gly
130 135 140
Leu Ile Ser Lys Ser Gly Thr Leu Thr Tyr Gln Met Met His Ala Leu
145 150 155 160
Ser Asp Val Gly Ile Ser Thr Ala Leu Gly Ile Gly Gly Asp Pro Leu
165 170 175
Ile Gly Thr Ala Tyr Ile Asp Ala Leu Ser Ala Phe Glu Glu Asp Pro
180 185 190
Asp Thr Arg Ala Ile Val Met Ile Gly Glu Ile Gly Gly Asp Ala Glu
195 200 205
Glu Arg Ala Ala Asp Tyr Ile Arg Glu His Val Thr Lys Pro Val Val
210 215 220
Gly Tyr Val Ala Gly Phe Thr Ala Pro Glu Gly Lys Thr Met Gly His
225 230 235 240
Ala Gly Ala Ile Val Thr Gly Ser Val Gly Thr Ala Gln Ala Lys Lys
245 250 255
Glu Ala Leu Glu Ala Ala Gly Val Arg Val Gly Thr Thr Pro Thr Glu
260 265 270
Thr Ala Glu Ile Met Arg Gly Ile Val Glu Gly Met
275 280
<210> 201
<211> 1569
<212> ДНК
<213> Corynebacterium humireducens
<220>
<221> CDS
<222> (301)..(1269)
<223> ген ddh
<400> 201
ggcgccaccc tagcgcctga gccggtttct gacacccgcg gatcctgcgg taccatctgc 60
aggcaggcgg gtacatgtgc ctgccggtgc tggagtggcg caatcggtag cgcaacggtc 120
ttgtaaaccg taggttgcga gttcaagtct cgtctccagc tctgctctcc acgaggtggg 180
gtccgggtga accggccccg cctcgttccc ttttgtggga ctcatttcta ttgactgacc 240
ggtgatgggc cgtagaatgg gcggcagcaa gcaccgtccc ccacctcaag gagtcagcac 300
atg gcc aac atc agg gtc gcg atc gtc ggt tac ggc aac ctc ggc aag 348
Met Ala Asn Ile Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Lys
1 5 10 15
tcc gtc gag aag ctc atc ggc cag cag ccg gac atg gag ctc gcg ggc 396
Ser Val Glu Lys Leu Ile Gly Gln Gln Pro Asp Met Glu Leu Ala Gly
20 25 30
gtg ttc tcg cgt cgc gac gca ctg gac acc ctc gac ccg gcc acc ccg 444
Val Phe Ser Arg Arg Asp Ala Leu Asp Thr Leu Asp Pro Ala Thr Pro
35 40 45
gtc ctg ccc gtc gcg gag ttc ggt gag cac acc gac aag ttc gac gtc 492
Val Leu Pro Val Ala Glu Phe Gly Glu His Thr Asp Lys Phe Asp Val
50 55 60
gcc gtc ctc tgc ctc ggc tcc gcc acc gac atc ccg gag cag gcc ccc 540
Ala Val Leu Cys Leu Gly Ser Ala Thr Asp Ile Pro Glu Gln Ala Pro
65 70 75 80
gag ttc atc cgc cac gcc aac acg gtc gac acc tac gac aac cac cgc 588
Glu Phe Ile Arg His Ala Asn Thr Val Asp Thr Tyr Asp Asn His Arg
85 90 95
gac atc ccg cgc cac cgc gcc gag atg gac agg gtg gcc agg gag aac 636
Asp Ile Pro Arg His Arg Ala Glu Met Asp Arg Val Ala Arg Glu Asn
100 105 110
ggc acc gtc gcc atc gtc tcc acc ggc tgg gac ccg ggc atg ttc tcc 684
Gly Thr Val Ala Ile Val Ser Thr Gly Trp Asp Pro Gly Met Phe Ser
115 120 125
ctc aac cgc acc atc ggc cag gcg ctc ctg ccg ggc ggc cag cag cag 732
Leu Asn Arg Thr Ile Gly Gln Ala Leu Leu Pro Gly Gly Gln Gln Gln
130 135 140
acc ttc tgg ggt ccg ggc ctc tcg cag ggc cac tct gac gcg gtg cgc 780
Thr Phe Trp Gly Pro Gly Leu Ser Gln Gly His Ser Asp Ala Val Arg
145 150 155 160
cgc gtc gcg ggc gtg aag aag ggc gtg cag tac acc atc ccc tcc ccg 828
Arg Val Ala Gly Val Lys Lys Gly Val Gln Tyr Thr Ile Pro Ser Pro
165 170 175
gag gcc ctc gag gcg gcc cgc aag ggt gag tcg ggt ctc agc ggc aag 876
Glu Ala Leu Glu Ala Ala Arg Lys Gly Glu Ser Gly Leu Ser Gly Lys
180 185 190
cag gcc cac ctg cgc cgc tgc ttc atc gtg gcg gac gag gcc gac cac 924
Gln Ala His Leu Arg Arg Cys Phe Ile Val Ala Asp Glu Ala Asp His
195 200 205
gag cgc atc gag aac gag atc cgc acc atg ccc gac tac ttc gtc ggc 972
Glu Arg Ile Glu Asn Glu Ile Arg Thr Met Pro Asp Tyr Phe Val Gly
210 215 220
tac gag gtg gag gtc aac ttc atc gac gag gcc acc ttc gac gcc gag 1020
Tyr Glu Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp Ala Glu
225 230 235 240
cac acc ggc atg ccg cac ggc ggg cac gtc atc acg gcg ggt gac acc 1068
His Thr Gly Met Pro His Gly Gly His Val Ile Thr Ala Gly Asp Thr
245 250 255
ggc ggc tac gtc cag tcc atc gag tac aac ctc gac ctg gac cgg aac 1116
Gly Gly Tyr Val Gln Ser Ile Glu Tyr Asn Leu Asp Leu Asp Arg Asn
260 265 270
ccg gac ttc acc ggt gcc gtc gcc ctg gca tac gcc cgt gcg gcc cac 1164
Pro Asp Phe Thr Gly Ala Val Ala Leu Ala Tyr Ala Arg Ala Ala His
275 280 285
cgt ctg cgt gag gcg ggc cag gcc ggc gcc tac acc gtc ctc gag gtc 1212
Arg Leu Arg Glu Ala Gly Gln Ala Gly Ala Tyr Thr Val Leu Glu Val
290 295 300
gcc ccg tac ctg atc tcc ccg acg ccg ctc gag gag ctc atc gcc cgc 1260
Ala Pro Tyr Leu Ile Ser Pro Thr Pro Leu Glu Glu Leu Ile Ala Arg
305 310 315 320
gac gtg tag ggtccaccac cgggatcagc aggagggcac cgaccagcag 1309
Asp Val
gaccaggccg atgccgagga tcccgaagcg gtcggtgccg cccagcagga cgaacatccc 1369
gaaggcgagc ggcgagagcc acgacacggc gcgtccggtg gtgacgtaca ggccgaagag 1429
ctggccctcc cgcccgtcgg gggcgacgcg ggagaggaac gagcgggccg ccgactgggc 1489
ggggccgacg aacaggcaca ggatgaggcc gaagatccag aacatctgcg ggccctccac 1549
gaagtagagg acgcccgcgg 1569
<210> 202
<211> 322
<212> БЕЛОК
<213> Corynebacterium humireducens
<400> 202
Met Ala Asn Ile Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Lys
1 5 10 15
Ser Val Glu Lys Leu Ile Gly Gln Gln Pro Asp Met Glu Leu Ala Gly
20 25 30
Val Phe Ser Arg Arg Asp Ala Leu Asp Thr Leu Asp Pro Ala Thr Pro
35 40 45
Val Leu Pro Val Ala Glu Phe Gly Glu His Thr Asp Lys Phe Asp Val
50 55 60
Ala Val Leu Cys Leu Gly Ser Ala Thr Asp Ile Pro Glu Gln Ala Pro
65 70 75 80
Glu Phe Ile Arg His Ala Asn Thr Val Asp Thr Tyr Asp Asn His Arg
85 90 95
Asp Ile Pro Arg His Arg Ala Glu Met Asp Arg Val Ala Arg Glu Asn
100 105 110
Gly Thr Val Ala Ile Val Ser Thr Gly Trp Asp Pro Gly Met Phe Ser
115 120 125
Leu Asn Arg Thr Ile Gly Gln Ala Leu Leu Pro Gly Gly Gln Gln Gln
130 135 140
Thr Phe Trp Gly Pro Gly Leu Ser Gln Gly His Ser Asp Ala Val Arg
145 150 155 160
Arg Val Ala Gly Val Lys Lys Gly Val Gln Tyr Thr Ile Pro Ser Pro
165 170 175
Glu Ala Leu Glu Ala Ala Arg Lys Gly Glu Ser Gly Leu Ser Gly Lys
180 185 190
Gln Ala His Leu Arg Arg Cys Phe Ile Val Ala Asp Glu Ala Asp His
195 200 205
Glu Arg Ile Glu Asn Glu Ile Arg Thr Met Pro Asp Tyr Phe Val Gly
210 215 220
Tyr Glu Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp Ala Glu
225 230 235 240
His Thr Gly Met Pro His Gly Gly His Val Ile Thr Ala Gly Asp Thr
245 250 255
Gly Gly Tyr Val Gln Ser Ile Glu Tyr Asn Leu Asp Leu Asp Arg Asn
260 265 270
Pro Asp Phe Thr Gly Ala Val Ala Leu Ala Tyr Ala Arg Ala Ala His
275 280 285
Arg Leu Arg Glu Ala Gly Gln Ala Gly Ala Tyr Thr Val Leu Glu Val
290 295 300
Ala Pro Tyr Leu Ile Ser Pro Thr Pro Leu Glu Glu Leu Ile Ala Arg
305 310 315 320
Asp Val
201400112A
1
<---

Claims (26)

1. Система расщепления глицина, содержащая ферменты GcvP, GcvT и GcvH, отличающаяся тем, что указанная система содержит по меньшей мере один из следующих полипептидов:
a) фермент GcvP с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvТ с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 42,
c) фермент GcvН с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 38.
2. Система расщепления глицина по п. 1, отличающаяся тем, что указанная система содержит два, предпочтительно все три из упомянутых выше полипептидов.
3. Система расщепления глицина по п. 2, отличающаяся тем, что указанная система содержит следующие полипептиды:
a) фермент GcvP с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvТ с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 42,
c) фермент GcvН с последовательностью, которая по меньшей мере на 95 или 98% идентична последовательности под SEQ ID NO: 38.
4. Система расщепления глицина по любому из предыдущих пунктов, отличающаяся тем, что указанная система содержит по меньшей мере один дополнительный полипептид, выбранный из группы, включающей:
a) фермент LipA с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 48,
b) фермент LipB с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 50,
c) фермент Lpd с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 52,
d) фермент LplA с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 94,
e) фермент GcvL с последовательностью, которая по меньшей мере на 90% идентична последовательности под SEQ ID NO: 96.
5. Полипептид, который представляет собой фермент системы расщепления глицина, выбранный из группы, включающей:
a) фермент GcvP с последовательностью, которая по меньшей мере на 90%, предпочтительно по меньшей мере на 92, 94, 96 или 98%, в особенности предпочтительно на 100% идентична последовательности под SEQ ID NO: 40,
b) фермент GcvT с последовательностью, которая по меньшей мере на 90%, предпочтительно по меньшей мере на 92, 94, 96 или 98%, в особенности предпочтительно на 100% идентична последовательности под SEQ ID NO: 42,
c) фермент GcvН с последовательностью, которая по меньшей мере на 90%, предпочтительно по меньшей мере на 92, 94, 96 или 98%, в особенности предпочтительно на 100% идентична последовательности под SEQ ID NO: 38.
6. Полинуклеотид, который кодирует фермент системы расщепления глицина, выбранный из группы, включающей:
a) полинуклеотид (gcvP), который кодирует фермент GcvP и имеет последовательность, которая по меньшей мере на 90 или 95%, предпочтительно по меньшей мере на 98 или 100% идентична последовательности в положениях 301-3162 под SEQ ID NO: 39, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-3162 под SEQ ID NO: 39;
b) полинуклеотид (gcvT), который кодирует фермент GcvT и имеет последовательность, которая по меньшей мере на 90 или 95%, предпочтительно по меньшей мере на 98 или 100% идентична последовательности в положениях 301-1404 под SEQ ID NO: 41, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-1404 под SEQ ID NO: 41;
c) полинуклеотид (gcvH), который кодирует фермент GcvН и имеет последовательность, которая по меньшей мере на 90 или 95%, предпочтительно по меньшей мере на 98 или 100% идентична последовательности в положениях 301-699 под SEQ ID NO: 37, и/или гибридизируется в жестких условиях с полинуклеотидом, последовательность которого является комплементарной последовательности в положениях 301-699 под SEQ ID NO: 37.
7. Экспрессионный вектор, отличающийся тем, что указанный вектор содержит по меньшей мере один полинуклеотид, предпочтительно по меньшей мере два или три полинуклеотида по п. 6.
8. Способ получения в избыточном количестве L-аминокислоты, отличающийся тем, что в указанном способе применяют систему расщепления глицина по любому из пп. 1-4, и/или по меньшей мере один полипептид по п. 5, и/или по меньшей мере один полинуклеотид по п. 6, и/или вектор по п. 7, где L-аминокислота выбрана из L-аланина, L-валина и L-аминокислот семейства глутамата.
9. Способ по п. 8, отличающийся тем, что в качестве побочного продукта образуются лишь незначительные количества глицина.
RU2016143585A 2014-04-30 2015-04-16 Способ получения l-аминокислот с помощью коринебактерий с применением системы расщепления глицина RU2713298C2 (ru)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
RU2792640C1 (ru) * 2021-04-07 2023-03-22 СиДжей ЧеилДжеданг Корпорейшн Новый вариант глицеральдегид-3-фосфатдегидрогеназы и способ получения l-валина с его применением

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016146633A1 (en) 2015-03-18 2016-09-22 Basf Se Recombinant microorganism for improved production of fine chemicals
CN108291243A (zh) 2015-11-27 2018-07-17 赢创德固赛有限公司 生产l-甲硫氨酸的方法
CN107227283B (zh) * 2017-05-26 2021-01-15 廊坊梅花生物技术开发有限公司 一种谷氨酸棒杆菌及其构建方法与应用
EP3456833A1 (en) * 2017-09-18 2019-03-20 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
CN109797126B (zh) * 2017-11-17 2021-07-06 中国科学院微生物研究所 生产l-丝氨酸的重组菌及其构建方法
KR101999454B1 (ko) * 2017-12-19 2019-07-11 씨제이제일제당 (주) L-아르기닌을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아르기닌 생산방법
RU2019128538A (ru) 2018-09-26 2021-03-11 Эвоник Оперейшенс ГмбХ Способ ферментативного получения l-лизина
KR102175112B1 (ko) * 2019-04-22 2020-11-06 씨제이제일제당 주식회사 L-쓰레오닌 생산능이 강화된 미생물 및 이를 이용한 쓰레오닌 생산방법
KR102204917B1 (ko) * 2019-04-22 2021-01-20 씨제이제일제당 주식회사 L-히스티딘 생산능이 강화된 미생물 및 이를 이용한 히스티딘 생산방법
US20210352902A1 (en) * 2020-05-15 2021-11-18 Psimos, Inc. Composition for growth stimulation and resistance to stress factors for plants of the cannabaceae family
CN112063572B (zh) * 2020-09-22 2022-07-19 浙江工业大学 一种高产o-乙酰-l-高丝氨酸的重组大肠杆菌及其应用
KR102281367B1 (ko) * 2021-01-26 2021-07-22 씨제이제일제당 (주) 신규한 5,10-메틸렌테트라하이드로폴레이트 리덕타제 변이체 및 이를 이용한 l-발린 생산 방법
KR102281370B1 (ko) * 2021-04-07 2021-07-22 씨제이제일제당 (주) 신규한 2-이소프로필말레이트합성효소 변이체 및 이를 이용한 l-발린 생산 방법
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WO2023166027A1 (en) * 2022-03-01 2023-09-07 Metabolic Explorer Microorganism and method for the improved production of leucine and/or isoleucine
KR102636674B1 (ko) * 2022-07-11 2024-02-15 대상 주식회사 L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
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KR20240108884A (ko) * 2022-12-30 2024-07-10 씨제이제일제당 (주) 신규한 bbd29_rs02010 변이체 및 이를 이용한 l-아르기닌 생산 방법
CN117512029B (zh) * 2024-01-03 2024-03-29 地奥集团成都药业股份有限公司 一种提升谷氨酰胺产量的培养基、方法及代谢组学分析方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008101857A2 (en) * 2007-02-19 2008-08-28 Evonik Degussa Gmbh Coryneform bacteria with formate-thf-synthetase and/or glycine cleavage activity
RU2010115225A (ru) * 2007-10-02 2011-11-10 Метаболик Эксплорер (Fr) Повышение выхода метионина

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1342308A (en) 1970-01-22 1974-01-03 Kyowa Hakko Kogyo Kk Production of threonine and lysine by fermentation
US5250434A (en) 1987-03-27 1993-10-05 Ajinomoto Co., Inc. Microorganisms for production of glutamic acid
DE3908201A1 (de) 1989-03-14 1990-09-27 Degussa Verfahren zur fermentativen herstellung von l-lysin
JP3023615B2 (ja) 1990-08-30 2000-03-21 協和醗酵工業株式会社 発酵法によるｌ―トリプトファンの製造法
DE4130867A1 (de) 1991-09-17 1993-03-18 Degussa Verfahren zur fermentativen herstellung von aminosaeuren
JPH05184366A (ja) 1992-01-14 1993-07-27 Mitsubishi Petrochem Co Ltd アスパルトキナーゼをコードする遺伝子ｄｎａ及びその利用
JP3473042B2 (ja) 1992-04-28 2003-12-02 味の素株式会社 変異型アスパルトキナーゼ遺伝子
JPH06261766A (ja) 1993-03-16 1994-09-20 Mitsubishi Petrochem Co Ltd フィードバックインヒビションの解除されたアスパルトキナーゼをコードする遺伝子ｄｎａ及びその利用
JPH0970291A (ja) 1995-06-30 1997-03-18 Ajinomoto Co Inc 人工トランスポゾンを用いた遺伝子増幅方法
DE19547361A1 (de) 1995-12-19 1997-06-26 Degussa Verfahren zur Herstellung von L-Aminosäuren durch Fermentation
JP4035855B2 (ja) 1996-06-05 2008-01-23 味の素株式会社 Ｌ−リジンの製造法
JP4075087B2 (ja) 1996-12-05 2008-04-16 味の素株式会社 Ｌ−リジンの製造法
JP4168463B2 (ja) 1996-12-05 2008-10-22 味の素株式会社 Ｌ−リジンの製造法
US5990350A (en) 1997-12-16 1999-11-23 Archer Midland Company Process for making granular L-lysine
KR100671785B1 (ko) 1999-04-19 2007-01-19 교와 핫꼬 고교 가부시끼가이샤 신규한 탈감작형 아스파르토키나아제
JPWO2002097086A1 (ja) * 2001-05-29 2005-04-07 協和醗酵工業株式会社 工業的生産に有用な微生物
AU2002355462A1 (en) 2001-08-06 2003-02-24 Degussa Ag Coryneform bacteria which produce chemical compounds ii
AU2002325923A1 (en) 2001-08-06 2003-05-19 Degussa Ag Production of l-lysine by genetically modified corynebacterium glutamicum strains
WO2005021772A1 (en) 2003-08-29 2005-03-10 Degussa Ag Process for the preparation of l-lysine
CN101578361A (zh) * 2005-06-17 2009-11-11 米克罗比亚精密工程股份有限公司 改进的氨基酸和代谢物生物合成

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008101857A2 (en) * 2007-02-19 2008-08-28 Evonik Degussa Gmbh Coryneform bacteria with formate-thf-synthetase and/or glycine cleavage activity
RU2010115225A (ru) * 2007-10-02 2011-11-10 Метаболик Эксплорер (Fr) Повышение выхода метионина

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KIKUCHI G. The glycine cleavage system: composition, reaction mechanism, and physiological significance. Molecular & Cellular Biochemistry, June 1973 Volume 1, number 2, pp. 169-187. *
WU C.-Y. ET AL. Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell. International Journal of Systematic and Evolutionary Microbiology (2011), 61, 882-887. *
WU C.-Y. ET AL. Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell. International Journal of Systematic and Evolutionary Microbiology (2011), 61, 882-887. KIKUCHI G. The glycine cleavage system: composition, reaction mechanism, and physiological significance. Molecular & Cellular Biochemistry, June 1973 Volume 1, number 2, pp. 169-187. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2792640C1 (ru) * 2021-04-07 2023-03-22 СиДжей ЧеилДжеданг Корпорейшн Новый вариант глицеральдегид-3-фосфатдегидрогеназы и способ получения l-валина с его применением

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